CmaCh12G013210 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G013210
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pre-mRNA cleavage factor Im 25 kDa subunit
Location	Cma_Chr12: 10105093 .. 10107350 (+)
RNA-Seq Expression	CmaCh12G013210
Synteny	CmaCh12G013210

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGCGGTATATTGCATTGGGTCTCGGCGTGCTGTCCGAAACAGAAAGCCCAAAAAAAAGACCTTGGGCCTCGGCGTGCTGTCCGAAACAGAAAGCCCAAAAAAAGCCCTTGGGCCTCGGCGTGCTGTCCGAAACAGAAAGCCCAAAAAAAGCCCATTTACTATTCCTCTTGCCGAGAAACCAAGTACCGTCATTCTGAAATTCTTTCTCTTCTTTCCTACTGGTCGTCTTGTCTGTCTCCTTTTAGCCTCATTAAGCACTGAGTGACGCACTCCACTTTTCGCTTCGTGTCCATTCTCTGCAACCAACACGCCATCTTCATCCACTTTCCTCCTTTTCTTCTGCTACTTCATCTCTCTTGATTTTCTACTGTTTTCCTCAATCGGCCATGGGAGACGACGGACGTATGCATGCTATGGAGGATGATGAATCTGCCGCTGCCGACCAAATCCGCGCCATCGATATTTACCCTCTCAGTAACTACTACTTCGGATCCAAAGAGCCTCTTATTTTCAAGGACAAGACCTTGGCTGATCGCGTTATCAGGATGAAATCCAAGTATCAAACCTCACTTTAATCCGTTGACTATACTGCGGCGAGGTTTCTGTTTACGTTTGTTGAGTTTGTCTAATATGTTTTCTCGGCTGTTTTTATTCAGTTATGCTGCTTATGGATTGAGGACTTCTGTCGAAGCAGTTTTGCTGGTTTGTTTTCACTTTATTCACGATAATTTTATTTGTTTCTGCTTTTATACGACTTCCATTTCTGCGACCTTGTGGTTCATACTGATTGATTTCTTCAAGCATATAGAAATTGTTACTCTCTCTGTTATTGCCGGTATTGAATTGCTTCACGCCTACTTTATCGGTGGAGTTCTGATACTGTTGGTTCGGAGTCGGTCTAGTCATATCGTATACCGCGATAAACACAGCCTCGCCTTATTTGACCTAGTCTCCATCCACTGTTGGATAGATCATAATTCCTGATTACTAGGAGTAATTAAAGCCACTTGCCCGTGTTGGCTGTTGTAAGACTAGTTCTTTATTTTGTCAAACGAACTTGATTTTTGCTTCTTATATTTGATCTCATTTCTTAAAGTCCATTTGGGGAAAAATCATGGCTAATTGGACGGCGCTGTTCATGAATTATTTTGGGATTTTTTATTTAATGATCATTGAATATGAGTTCAAGATGTTTTAATTTTCTGCTATATGATCTCTAAATGGACATTTCATCTTGCGATCCGAAACAGTGCTTAGCTACTGTATTGTAATTGCAAGCATCTGTTGTTCTTAAATTAGGTTGAGCTATTTAAGCATCCTCATGTGTTGCTGTTGCAAATACGAAATTCAATTTTCAGTCTTCCCGGTGGTCGCCTAAGGCCCAATGAATCTGGTAAATGTGTGCCATAAATCTCTTCTGCAGCTTTCCGTTGTCTAATAGTTACACTTATGGATATTTTATTATGGTGGATGATCACAAATTACAGATATTGATGGCTTGAAACGAAAGTTATCAAGGAAGCTTTCTGCCAATGGATCCCCTGATGCATCTGATTGGGAGGTATTATAATGCCCAATTGTATTTCCTTTTTCTTAGTTACTACCATGTGGCCTCTGATTATGAAAGATGGAATCTGAAGTTTTCTTCTAATGTTTAGGTGGGCGATTGCCTCGGTACGTGGTGGAGGCCTGACTTCGAAACCTTGCTCAATCCATATTTGTCCAACAATGTAAAAGGAGCCAAGGTAGTTATTTGGATACAAACTACTTTCTTTGAACTATGACTACTGGTGGGCGATATACCATTTACCTGTAACTCACTCACTACTTGTTGCAGGAGTGCACAAAACTTTTCCTGGTCAAATTGCCGGAGAGTCGAAAATTCATTGTGCCAAAGAACCTCAAGTTGATTGCAGTTCCTTTATGCCAACTTCACGAAAACCATAAGGTATCATCCTAATCAAGGCACTCTGTGGAAGTCCAATTTGTGACTACAAATCTTACCTTCCTTTAATTGAAAGAAAAGAAAAGGAAAAAAAAAAAAGTTCTATTTGGTTGTGGAAATTCTTAAAATAAATCTCTATGGTTGAAACGTGGTCCATAACCCAACATACATGTGAATATCTCAAACATCTTTGACACATACAACGCTTCCTTCTCATATTAATCTTCTCATTTTGCAGATGTACGGGCCAGTTATATCGGGTATTCCCCAGTTACTTTCCAAGTTTTCCTTCAACATCATCGAAACTTAA

mRNA sequence

ATGGGCGCACTGAGTGACGCACTCCACTTTTCGCTTCGTGTCCATTCTCTGCAACCAACACGCCATCTTCATCCACTTTCCTCCTTTTCTTCTGCTACTTCATCTCTCTTGATTTTCTACTGTTTTCCTCAATCGGCCATGGGAGACGACGGACGTATGCATGCTATGGAGGATGATGAATCTGCCGCTGCCGACCAAATCCGCGCCATCGATATTTACCCTCTCAGTAACTACTACTTCGGATCCAAAGAGCCTCTTATTTTCAAGGACAAGACCTTGGCTGATCGCGTTATCAGGATGAAATCCAATTATGCTGCTTATGGATTGAGGACTTCTGTCGAAGCAGTTTTGCTGGTTGAGCTATTTAAGCATCCTCATGTGTTGCTGTTGCAAATACGAAATTCAATTTTCAGTCTTCCCGGTGGTCGCCTAAGGCCCAATGAATCTGATATTGATGGCTTGAAACGAAAGTTATCAAGGAAGCTTTCTGCCAATGGATCCCCTGATGCATCTGATTGGGAGGTGGGCGATTGCCTCGGTACGTGGTGGAGGCCTGACTTCGAAACCTTGCTCAATCCATATTTGTCCAACAATGTAAAAGGAGCCAAGGAGTGCACAAAACTTTTCCTGGTCAAATTGCCGGAGAGTCGAAAATTCATTGTGCCAAAGAACCTCAAGTTGATTGCAGTTCCTTTATGCCAACTTCACGAAAACCATAAGATGTACGGGCCAGTTATATCGGGTATTCCCCAGTTACTTTCCAAGTTTTCCTTCAACATCATCGAAACTTAA

Coding sequence (CDS)

ATGGGCGCACTGAGTGACGCACTCCACTTTTCGCTTCGTGTCCATTCTCTGCAACCAACACGCCATCTTCATCCACTTTCCTCCTTTTCTTCTGCTACTTCATCTCTCTTGATTTTCTACTGTTTTCCTCAATCGGCCATGGGAGACGACGGACGTATGCATGCTATGGAGGATGATGAATCTGCCGCTGCCGACCAAATCCGCGCCATCGATATTTACCCTCTCAGTAACTACTACTTCGGATCCAAAGAGCCTCTTATTTTCAAGGACAAGACCTTGGCTGATCGCGTTATCAGGATGAAATCCAATTATGCTGCTTATGGATTGAGGACTTCTGTCGAAGCAGTTTTGCTGGTTGAGCTATTTAAGCATCCTCATGTGTTGCTGTTGCAAATACGAAATTCAATTTTCAGTCTTCCCGGTGGTCGCCTAAGGCCCAATGAATCTGATATTGATGGCTTGAAACGAAAGTTATCAAGGAAGCTTTCTGCCAATGGATCCCCTGATGCATCTGATTGGGAGGTGGGCGATTGCCTCGGTACGTGGTGGAGGCCTGACTTCGAAACCTTGCTCAATCCATATTTGTCCAACAATGTAAAAGGAGCCAAGGAGTGCACAAAACTTTTCCTGGTCAAATTGCCGGAGAGTCGAAAATTCATTGTGCCAAAGAACCTCAAGTTGATTGCAGTTCCTTTATGCCAACTTCACGAAAACCATAAGATGTACGGGCCAGTTATATCGGGTATTCCCCAGTTACTTTCCAAGTTTTCCTTCAACATCATCGAAACTTAA

Protein sequence

MGALSDALHFSLRVHSLQPTRHLHPLSSFSSATSSLLIFYCFPQSAMGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAAYGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANGSPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET

Homology

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BLAST of CmaCh12G013210 vs. ExPASy Swiss-Prot
Match: Q94AF0 (Pre-mRNA cleavage factor Im 25 kDa subunit 1 OS=Arabidopsis thaliana OX=3702 GN=CFIS1 PE=1 SV=1)

HSP 1 Score: 299.7 bits (766), Expect = 3.3e-80
Identity = 144/221 (65.16%), Postives = 178/221 (80.54%), Query Frame = 0


Query: 47  MGDDGR---MHAMEDDESAAADQIR--AIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMK 106
           MG++ R   M  + D+ +   D +    +D+YPLS+YYFGSKE L  KD+ ++DRVIR+K
Sbjct: 1   MGEEARALDMEEISDNTTRRNDVVHDLMVDLYPLSSYYFGSKEALRVKDEIISDRVIRLK 60

Query: 107 SNYAAYGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRK 166
           SNYAA+GLRT VEAVLLVELFKHPHVLLLQ RNSIF LPGGRLRP ESDI+GLKRKL+ K
Sbjct: 61  SNYAAHGLRTCVEAVLLVELFKHPHVLLLQYRNSIFKLPGGRLRPGESDIEGLKRKLASK 120

Query: 167 LSANGSPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIV 226
           LS N +   S +EVG+C+G WWRP+FETL+ P+L  N+K  KECTKLFLV+LP  ++F+V
Sbjct: 121 LSVNENVGVSGYEVGECIGMWWRPNFETLMYPFLPPNIKHPKECTKLFLVRLPVHQQFVV 180

Query: 227 PKNLKLIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIE 263
           PKN KL+AVPLCQLHEN K YGP++S IP+LLSKFSFN++E
Sbjct: 181 PKNFKLLAVPLCQLHENEKTYGPIMSQIPKLLSKFSFNMME 221

[Close]

BLAST of CmaCh12G013210 vs. ExPASy Swiss-Prot
Match: Q8GXS3 (Pre-mRNA cleavage factor Im 25 kDa subunit 2 OS=Arabidopsis thaliana OX=3702 GN=CFIS2 PE=1 SV=1)

HSP 1 Score: 237.3 bits (604), Expect = 2.0e-61
Identity = 108/196 (55.10%), Postives = 144/196 (73.47%), Query Frame = 0


Query: 68  RAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAAYGLRTSVEAVLLVELFKHPHV 127
           + ++ YPLSNY FG+KEP + KD ++ADR+ RMK NY   G+RTSVE +LLV+   HPH+
Sbjct: 5   QVVNTYPLSNYSFGTKEPKLEKDTSVADRLARMKINYMKEGMRTSVEGILLVQEHNHPHI 64

Query: 128 LLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANGSPDASDWEVGDCLGTWWRPDF 187
           LLLQI N+   LPGGRL+P E++ DGLKRKL+ KL  N +    DW VG+C+ TWWRP+F
Sbjct: 65  LLLQIGNTFCKLPGGRLKPGENEADGLKRKLTSKLGGNSAALVPDWTVGECVATWWRPNF 124

Query: 188 ETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENHKMYGPVIS 247
           ET++ PY   ++   KEC +L++V L E   F VPKNLKL+AVPL +L++N + YGPVIS
Sbjct: 125 ETMMYPYCPPHITKPKECKRLYIVHLSEKEYFAVPKNLKLLAVPLFELYDNVQRYGPVIS 184

Query: 248 GIPQLLSKFSFNIIET 264
            IPQ LS+F FN+I +
Sbjct: 185 TIPQQLSRFHFNMISS 200

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BLAST of CmaCh12G013210 vs. ExPASy Swiss-Prot
Match: Q7T3C6 (Cleavage and polyadenylation specificity factor subunit 5 OS=Danio rerio OX=7955 GN=nudt21 PE=2 SV=1)

HSP 1 Score: 194.9 bits (494), Expect = 1.1e-48
Identity = 92/191 (48.17%), Postives = 126/191 (65.97%), Query Frame = 0


Query: 68  RAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAAYGLRTSVEAVLLVELFKHPHV 127
           R I++YPL+NY FG+KEPL  KD ++A R  RM+  +   G+R +VE VL+V   + PHV
Sbjct: 36  RTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFEKIGMRRTVEGVLIVHEHRLPHV 95

Query: 128 LLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANGSPDASDWEVGDCLGTWWRPDF 187
           LLLQ+  + F LPGG L P E +++GLKR ++  L         DW + DC+G WWRP+F
Sbjct: 96  LLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGV-KQDWVIDDCIGNWWRPNF 155

Query: 188 ETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENHKMYGPVIS 247
           E    PY+  ++   KE  KLFLV+L E   F VPKN KL+A PL +L++N   YGP+IS
Sbjct: 156 EPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIIS 215

Query: 248 GIPQLLSKFSF 259
            +PQLLS+F+F
Sbjct: 216 SLPQLLSRFNF 225

[Close]

BLAST of CmaCh12G013210 vs. ExPASy Swiss-Prot
Match: Q3ZCA2 (Cleavage and polyadenylation specificity factor subunit 5 OS=Bos taurus OX=9913 GN=NUDT21 PE=2 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.5e-48
Identity = 92/191 (48.17%), Postives = 126/191 (65.97%), Query Frame = 0


Query: 68  RAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAAYGLRTSVEAVLLVELFKHPHV 127
           R I++YPL+NY FG+KEPL  KD ++A R  RM+  +   G+R +VE VL+V   + PHV
Sbjct: 35  RTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHV 94

Query: 128 LLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANGSPDASDWEVGDCLGTWWRPDF 187
           LLLQ+  + F LPGG L P E +++GLKR ++  L         DW + DC+G WWRP+F
Sbjct: 95  LLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGV-LQDWVIDDCIGNWWRPNF 154

Query: 188 ETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENHKMYGPVIS 247
           E    PY+  ++   KE  KLFLV+L E   F VPKN KL+A PL +L++N   YGP+IS
Sbjct: 155 EPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIIS 214

Query: 248 GIPQLLSKFSF 259
            +PQLLS+F+F
Sbjct: 215 SLPQLLSRFNF 224

[Close]

BLAST of CmaCh12G013210 vs. ExPASy Swiss-Prot
Match: O43809 (Cleavage and polyadenylation specificity factor subunit 5 OS=Homo sapiens OX=9606 GN=NUDT21 PE=1 SV=1)

HSP 1 Score: 194.5 bits (493), Expect = 1.5e-48
Identity = 92/191 (48.17%), Postives = 126/191 (65.97%), Query Frame = 0


Query: 68  RAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAAYGLRTSVEAVLLVELFKHPHV 127
           R I++YPL+NY FG+KEPL  KD ++A R  RM+  +   G+R +VE VL+V   + PHV
Sbjct: 35  RTINLYPLTNYTFGTKEPLYEKDSSVAARFQRMREEFDKIGMRRTVEGVLIVHEHRLPHV 94

Query: 128 LLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANGSPDASDWEVGDCLGTWWRPDF 187
           LLLQ+  + F LPGG L P E +++GLKR ++  L         DW + DC+G WWRP+F
Sbjct: 95  LLLQLGTTFFKLPGGELNPGEDEVEGLKRLMTEILGRQDGV-LQDWVIDDCIGNWWRPNF 154

Query: 188 ETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENHKMYGPVIS 247
           E    PY+  ++   KE  KLFLV+L E   F VPKN KL+A PL +L++N   YGP+IS
Sbjct: 155 EPPQYPYIPAHITKPKEHKKLFLVQLQEKALFAVPKNYKLVAAPLFELYDNAPGYGPIIS 214

Query: 248 GIPQLLSKFSF 259
            +PQLLS+F+F
Sbjct: 215 SLPQLLSRFNF 224

[Close]

BLAST of CmaCh12G013210 vs. ExPASy TrEMBL
Match: A0A6J1HNY2 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita maxima OX=3661 GN=LOC111465348 PE=3 SV=1)

HSP 1 Score: 444.9 bits (1143), Expect = 2.3e-121
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 47  MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 106
           MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 60

Query: 107 YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 166
           YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 167 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 226
           SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 227 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 264
           LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 217

[Close]

BLAST of CmaCh12G013210 vs. ExPASy TrEMBL
Match: A0A6J1FHN4 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita moschata OX=3662 GN=LOC111444047 PE=3 SV=1)

HSP 1 Score: 436.4 bits (1121), Expect = 8.4e-119
Identity = 212/217 (97.70%), Postives = 216/217 (99.54%), Query Frame = 0


Query: 47  MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 106
           MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 60

Query: 107 YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 166
           YGLRTSVEAVLLVELFKHPHVLLLQIRNSIF+LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFNLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 167 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 226
           +PDASDWEVGDCLGTWWRPDFETLL P+LSNNVKGAKECTKLFLVKLPE+RKFIVPKNLK
Sbjct: 121 APDASDWEVGDCLGTWWRPDFETLLYPHLSNNVKGAKECTKLFLVKLPENRKFIVPKNLK 180

Query: 227 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 264
           LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 217

[Close]

BLAST of CmaCh12G013210 vs. ExPASy TrEMBL
Match: A0A6J1DN47 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Momordica charantia OX=3673 GN=LOC111021937 PE=3 SV=1)

HSP 1 Score: 391.3 bits (1004), Expect = 3.1e-105
Identity = 188/216 (87.04%), Postives = 202/216 (93.52%), Query Frame = 0


Query: 47  MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 106
           MGD+G +HAMEDDE+A A+Q RA+ IYPL NYYFGSKEPL+FK++TLADR+ RMKSNYAA
Sbjct: 1   MGDNGHLHAMEDDEAATAEQSRAVRIYPLGNYYFGSKEPLLFKNETLADRLHRMKSNYAA 60

Query: 107 YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 166
            GLRT VEAVLLVELFKHPH+LLLQIRNSIF LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  NGLRTCVEAVLLVELFKHPHLLLLQIRNSIFKLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 167 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 226
           + DASDWEVG+CLG WWRPDFETLL PYLS+NVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 TSDASDWEVGECLGMWWRPDFETLLYPYLSSNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 227 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIE 263
           LIAVPLCQLHEN K YGPVISG+PQLLSKFSFNIIE
Sbjct: 181 LIAVPLCQLHENQKTYGPVISGVPQLLSKFSFNIIE 216

[Close]

BLAST of CmaCh12G013210 vs. ExPASy TrEMBL
Match: A0A0A0LIM0 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis sativus OX=3659 GN=Csa_2G000310 PE=3 SV=1)

HSP 1 Score: 384.4 bits (986), Expect = 3.8e-103
Identity = 179/217 (82.49%), Postives = 202/217 (93.09%), Query Frame = 0


Query: 47  MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 106
           MGDDGR+HA++DD++++A Q+  IDIYPLSNYYFGSKEPL+FKD+TL+DRV+RMKSNYAA
Sbjct: 1   MGDDGRLHAIDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRVLRMKSNYAA 60

Query: 107 YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 166
           +GLRT VEAV+LVELFKHPH+LL QIRNSIF LPGGR+RPNESDIDGL RKL++KLSANG
Sbjct: 61  HGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRIRPNESDIDGLTRKLTKKLSANG 120

Query: 167 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 226
           + DAS WEV +CLG WWRPDFETLL PYLS NVKGAKECTKLFLVKLPESRKF+VPKNLK
Sbjct: 121 ASDASHWEVSECLGMWWRPDFETLLFPYLSTNVKGAKECTKLFLVKLPESRKFVVPKNLK 180

Query: 227 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 264
           LIAVPLCQ+HENHK YGP+ISGIPQLLSKFSFNII T
Sbjct: 181 LIAVPLCQIHENHKTYGPIISGIPQLLSKFSFNIIGT 217

[Close]

BLAST of CmaCh12G013210 vs. ExPASy TrEMBL
Match: A0A1S3C9Z9 (Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis melo OX=3656 GN=LOC103498651 PE=3 SV=1)

HSP 1 Score: 380.9 bits (977), Expect = 4.2e-102
Identity = 180/219 (82.19%), Postives = 201/219 (91.78%), Query Frame = 0


Query: 47  MGDDGRMHAM--EDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNY 106
           MGDDGR+HA+  +DD++++A Q+  IDIYPLSNYYFGSKEPL+FKD+TL+DR++RMKSNY
Sbjct: 1   MGDDGRLHAIDDDDDDASSAHQLSYIDIYPLSNYYFGSKEPLLFKDETLSDRILRMKSNY 60

Query: 107 AAYGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSA 166
           AAYGLRT VEAV+LVELFKHPH+LL QIRNSIF LPGGRLRPNESDIDGL RKLS+KL A
Sbjct: 61  AAYGLRTCVEAVMLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDIDGLTRKLSKKLCA 120

Query: 167 NGSPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKN 226
           NG+ DASDWEV +CLG WWRPDFETLL PYLS NVKGAKEC KLFLVKLPES+KF+VPKN
Sbjct: 121 NGASDASDWEVNECLGMWWRPDFETLLFPYLSTNVKGAKECAKLFLVKLPESKKFVVPKN 180

Query: 227 LKLIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 264
           LKLIAVPLCQ+HENHK YGPVISGIPQLLSKFSFNII T
Sbjct: 181 LKLIAVPLCQIHENHKTYGPVISGIPQLLSKFSFNIIGT 219

[Close]

BLAST of CmaCh12G013210 vs. NCBI nr
Match: XP_022965443.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita maxima])

HSP 1 Score: 444.9 bits (1143), Expect = 4.9e-121
Identity = 217/217 (100.00%), Postives = 217/217 (100.00%), Query Frame = 0


Query: 47  MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 106
           MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 60

Query: 107 YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 166
           YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 167 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 226
           SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 227 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 264
           LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 217

[Close]

BLAST of CmaCh12G013210 vs. NCBI nr
Match: KAG6586493.1 (Pre-mRNA cleavage factor Im 25 kDa subunit 1, partial [Cucurbita argyrosperma subsp. sororia] >KAG7021351.1 Pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 439.1 bits (1128), Expect = 2.7e-119
Identity = 213/217 (98.16%), Postives = 216/217 (99.54%), Query Frame = 0


Query: 47  MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 106
           MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRV+RMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVVRMKSNYAA 60

Query: 107 YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 166
           YGLRTSVEAVLLVELFKHPHVLLLQIRNSIF+LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFNLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 167 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 226
           +PDASDWEVGDCLGTWWRPDFETLL PYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 APDASDWEVGDCLGTWWRPDFETLLYPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 227 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 264
           LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 217

[Close]

BLAST of CmaCh12G013210 vs. NCBI nr
Match: XP_022937740.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita moschata])

HSP 1 Score: 436.4 bits (1121), Expect = 1.7e-118
Identity = 212/217 (97.70%), Postives = 216/217 (99.54%), Query Frame = 0


Query: 47  MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 106
           MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 60

Query: 107 YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 166
           YGLRTSVEAVLLVELFKHPHVLLLQIRNSIF+LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFNLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 167 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 226
           +PDASDWEVGDCLGTWWRPDFETLL P+LSNNVKGAKECTKLFLVKLPE+RKFIVPKNLK
Sbjct: 121 APDASDWEVGDCLGTWWRPDFETLLYPHLSNNVKGAKECTKLFLVKLPENRKFIVPKNLK 180

Query: 227 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 264
           LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 217

[Close]

BLAST of CmaCh12G013210 vs. NCBI nr
Match: XP_023536755.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 435.6 bits (1119), Expect = 2.9e-118
Identity = 211/217 (97.24%), Postives = 215/217 (99.08%), Query Frame = 0


Query: 47  MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 106
           MGDDGRMHAMEDDESA ADQIRAIDIYPLSNYYFGSKEPL+FKDKTLADRVIRMKSNYAA
Sbjct: 1   MGDDGRMHAMEDDESATADQIRAIDIYPLSNYYFGSKEPLVFKDKTLADRVIRMKSNYAA 60

Query: 107 YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 166
           YGLRTSVEAVLLVELFKHPHVLLLQIRNSIF+LPGGRLRPNESDIDGLKRKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFNLPGGRLRPNESDIDGLKRKLSRKLSANG 120

Query: 167 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 226
           +PDASDWEVGDCLGTWWRPDFETLL P+LSNNVKGAKECTKLFLVKLPESRKFIVPKNLK
Sbjct: 121 APDASDWEVGDCLGTWWRPDFETLLYPHLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 180

Query: 227 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 264
           LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET
Sbjct: 181 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 217

[Close]

BLAST of CmaCh12G013210 vs. NCBI nr
Match: XP_038889512.1 (pre-mRNA cleavage factor Im 25 kDa subunit 1 [Benincasa hispida])

HSP 1 Score: 394.0 bits (1011), Expect = 9.8e-106
Identity = 187/217 (86.18%), Postives = 204/217 (94.01%), Query Frame = 0


Query: 47  MGDDGRMHAMEDDESAAADQIRAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAA 106
           MGDDGR+HAM+DDE++AADQ  +I IYPLSNYYF SKEPL+FKD+T ADR++RMKSNYAA
Sbjct: 1   MGDDGRLHAMDDDEASAADQRPSIHIYPLSNYYFESKEPLLFKDETFADRLLRMKSNYAA 60

Query: 107 YGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANG 166
           YGLRTSVEAVLLVELFKHPH+LL QIRNSIF LPGGRLRPNESD+DGL RKLSRKLSANG
Sbjct: 61  YGLRTSVEAVLLVELFKHPHLLLFQIRNSIFKLPGGRLRPNESDVDGLTRKLSRKLSANG 120

Query: 167 SPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLK 226
           + DASDWEVG+CLG WWRPDFETLL PYLS+ VKGAKECTKLFLVKLPESRKF+VPKNLK
Sbjct: 121 ASDASDWEVGECLGMWWRPDFETLLFPYLSSTVKGAKECTKLFLVKLPESRKFVVPKNLK 180

Query: 227 LIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIET 264
           LIAVPLCQ+HENHK YGPVISGIPQLLSKFSF+IIET
Sbjct: 181 LIAVPLCQIHENHKTYGPVISGIPQLLSKFSFDIIET 217

[Close]

BLAST of CmaCh12G013210 vs. TAIR 10
Match: AT4G29820.1 (homolog of CFIM-25 )

HSP 1 Score: 299.7 bits (766), Expect = 2.3e-81
Identity = 144/221 (65.16%), Postives = 178/221 (80.54%), Query Frame = 0


Query: 47  MGDDGR---MHAMEDDESAAADQIR--AIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMK 106
           MG++ R   M  + D+ +   D +    +D+YPLS+YYFGSKE L  KD+ ++DRVIR+K
Sbjct: 1   MGEEARALDMEEISDNTTRRNDVVHDLMVDLYPLSSYYFGSKEALRVKDEIISDRVIRLK 60

Query: 107 SNYAAYGLRTSVEAVLLVELFKHPHVLLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRK 166
           SNYAA+GLRT VEAVLLVELFKHPHVLLLQ RNSIF LPGGRLRP ESDI+GLKRKL+ K
Sbjct: 61  SNYAAHGLRTCVEAVLLVELFKHPHVLLLQYRNSIFKLPGGRLRPGESDIEGLKRKLASK 120

Query: 167 LSANGSPDASDWEVGDCLGTWWRPDFETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIV 226
           LS N +   S +EVG+C+G WWRP+FETL+ P+L  N+K  KECTKLFLV+LP  ++F+V
Sbjct: 121 LSVNENVGVSGYEVGECIGMWWRPNFETLMYPFLPPNIKHPKECTKLFLVRLPVHQQFVV 180

Query: 227 PKNLKLIAVPLCQLHENHKMYGPVISGIPQLLSKFSFNIIE 263
           PKN KL+AVPLCQLHEN K YGP++S IP+LLSKFSFN++E
Sbjct: 181 PKNFKLLAVPLCQLHENEKTYGPIMSQIPKLLSKFSFNMME 221

[Close]

BLAST of CmaCh12G013210 vs. TAIR 10
Match: AT4G25550.1 (Cleavage/polyadenylation specificity factor, 25kDa subunit )

HSP 1 Score: 237.3 bits (604), Expect = 1.4e-62
Identity = 108/196 (55.10%), Postives = 144/196 (73.47%), Query Frame = 0


Query: 68  RAIDIYPLSNYYFGSKEPLIFKDKTLADRVIRMKSNYAAYGLRTSVEAVLLVELFKHPHV 127
           + ++ YPLSNY FG+KEP + KD ++ADR+ RMK NY   G+RTSVE +LLV+   HPH+
Sbjct: 5   QVVNTYPLSNYSFGTKEPKLEKDTSVADRLARMKINYMKEGMRTSVEGILLVQEHNHPHI 64

Query: 128 LLLQIRNSIFSLPGGRLRPNESDIDGLKRKLSRKLSANGSPDASDWEVGDCLGTWWRPDF 187
           LLLQI N+   LPGGRL+P E++ DGLKRKL+ KL  N +    DW VG+C+ TWWRP+F
Sbjct: 65  LLLQIGNTFCKLPGGRLKPGENEADGLKRKLTSKLGGNSAALVPDWTVGECVATWWRPNF 124

Query: 188 ETLLNPYLSNNVKGAKECTKLFLVKLPESRKFIVPKNLKLIAVPLCQLHENHKMYGPVIS 247
           ET++ PY   ++   KEC +L++V L E   F VPKNLKL+AVPL +L++N + YGPVIS
Sbjct: 125 ETMMYPYCPPHITKPKECKRLYIVHLSEKEYFAVPKNLKLLAVPLFELYDNVQRYGPVIS 184

Query: 248 GIPQLLSKFSFNIIET 264
            IPQ LS+F FN+I +
Sbjct: 185 TIPQQLSRFHFNMISS 200

The following BLAST results are available for this feature:

BLAST of CmaCh12G013210 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q94AF0	3.3e-80	65.16	Pre-mRNA cleavage factor Im 25 kDa subunit 1 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q8GXS3	2.0e-61	55.10	Pre-mRNA cleavage factor Im 25 kDa subunit 2 OS=Arabidopsis thaliana OX=3702 GN=...	[more]
Q7T3C6	1.1e-48	48.17	Cleavage and polyadenylation specificity factor subunit 5 OS=Danio rerio OX=7955...	[more]
Q3ZCA2	1.5e-48	48.17	Cleavage and polyadenylation specificity factor subunit 5 OS=Bos taurus OX=9913 ...	[more]
O43809	1.5e-48	48.17	Cleavage and polyadenylation specificity factor subunit 5 OS=Homo sapiens OX=960...	[more]

BLAST of CmaCh12G013210 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HNY2	2.3e-121	100.00	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita maxima OX=3661 GN=LOC111...	[more]
A0A6J1FHN4	8.4e-119	97.70	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucurbita moschata OX=3662 GN=LOC1...	[more]
A0A6J1DN47	3.1e-105	87.04	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Momordica charantia OX=3673 GN=LOC...	[more]
A0A0A0LIM0	3.8e-103	82.49	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis sativus OX=3659 GN=Csa_2G0...	[more]
A0A1S3C9Z9	4.2e-102	82.19	Pre-mRNA cleavage factor Im 25 kDa subunit OS=Cucumis melo OX=3656 GN=LOC1034986...	[more]

BLAST of CmaCh12G013210 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022965443.1	4.9e-121	100.00	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita maxima]	[more]
KAG6586493.1	2.7e-119	98.16	Pre-mRNA cleavage factor Im 25 kDa subunit 1, partial [Cucurbita argyrosperma su...	[more]
XP_022937740.1	1.7e-118	97.70	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita moschata]	[more]
XP_023536755.1	2.9e-118	97.24	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Cucurbita pepo subsp. pepo]	[more]
XP_038889512.1	9.8e-106	86.18	pre-mRNA cleavage factor Im 25 kDa subunit 1 [Benincasa hispida]	[more]

BLAST of CmaCh12G013210 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G29820.1	2.3e-81	65.16	homolog of CFIM-25	[more]
AT4G25550.1	1.4e-62	55.10	Cleavage/polyadenylation specificity factor, 25kDa subunit	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PFAM	PF13869	NUDIX_2	coord: 69..256 e-value: 1.2E-70 score: 237.0
IPR016706	Cleavage/polyadenylation specificity factor subunit 5	PANTHER	PTHR13047	PRE-MRNA CLEAVAGE FACTOR IM, 25KD SUBUNIT	coord: 50..262
None	No IPR available	GENE3D	3.90.79.10	Nucleoside Triphosphate Pyrophosphohydrolase	coord: 68..260 e-value: 7.3E-75 score: 252.6
None	No IPR available	PANTHER	PTHR13047:SF1	PRE-MRNA CLEAVAGE FACTOR IM 25 KDA SUBUNIT 1	coord: 50..262
IPR000086	NUDIX hydrolase domain	PROSITE	PS51462	NUDIX	coord: 108..234 score: 9.028849
IPR015797	NUDIX hydrolase-like domain superfamily	SUPERFAMILY	55811	Nudix	coord: 95..158

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G013210.1	CmaCh12G013210.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0006378	mRNA polyadenylation
biological_process	GO:0006457	protein folding
cellular_component	GO:0005832	chaperonin-containing T-complex
cellular_component	GO:0005849	mRNA cleavage factor complex
molecular_function	GO:0005524	ATP binding
molecular_function	GO:0016887	ATP hydrolysis activity
molecular_function	GO:0003729	mRNA binding
molecular_function	GO:0051082	unfolded protein binding