CmaCh12G012850 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G012850
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionvacuolar protein sorting-associated protein 32 homolog 2
LocationCma_Chr12: 9988233 .. 9991945 (+)
RNA-Seq ExpressionCmaCh12G012850
SyntenyCmaCh12G012850
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AAATAGATTGCCGCGTGTGGTGGACAAACAGCGACGGGAAGAGAGGGAACTCCAAATCTTCGTCCTTCCTTTTCTGGAGCGCCTTGGAAGAAACCCTTTCTACTTCAAATTTCTTCACAAATTCTTTCATTTCATTCTCGTTGGTATAAATTTGTCTCTGATTATCATCTTTCCGTTTCAATCTCTTGCTGGTTTCTCGATTTCTTTCTGGTTGGCATTGATTTTTTTATTTTTTTTATTTTTTTTATTTTTAAAAATCGTGTCGAAGTTGATCGGCATGGCGATTTCTCTTCTTGAGGAATTCATTTTGTCTGTGATTTTGTTGTTGCAGATCGAGGGGCGATTCTTTTTTCTTAGCTTAAACAATGTTAAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGACAAGTTGAACGAGGTATGTTTTACTTCGATGTTTTATTGTTTTTGATATTCTTTGTATGGAAATGATCTTTTCTTTTTCTCACTTGTTCTGAACCTATTTCATCGATCTTGATGGATGCTTGACTTCTCGATCATTCTTCTTTCCTTGCCTCATCCAAATATTAGTGTCTATGACGACGTAGTAATTGGTCCTACTTGACGGAGTATTTGTGTTTATGACGTCGTATTAACTCTCCTTGATGGAGTACTAGTGTTTATGGCGTCGTAATAATTGGTTCTCCTTGACCGAGTATTAGTGTTTATAATGTCGTAGTTATTGGTTTCCAGTTGAAATTATCGTCTTATTTGGATTTGTTAACTCGTAATTTTACATTTGTCTTGCCACTGGTAACTGCCGTTCTCTAGGTGAAATGTCGCTTATGTAAGAAAGGAAGCTTGTGTACAACAATGCTGTGTTTTTATTCCATGCGGATATTTGGAATTGCGTCTAAAATATTGGTTGCGTAGATTTTTGTAGTCCGACTAAGCTTTTCATAATTGATACCGATATTTTTGTGCAATTTATTGTCCGGCGTTGTTTGATTTAATTGCTTCCTTTTCATCGTCTGGGATTATCTATTTAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTATTAAGAAGGCTGCTGCAGAGGTTGAAAAAGCCAAAGAATTCACAAGAGTGAAGAATAAACGAGGTATGTTATGTAATTGGGTGATCTCTTCTAATGGTCAAATGCTTCTTGGTATTAATTTTTATTCCTTTTTTGAGTATTTGTTCACATGTTAAGTGTGAATAAGATTTAATTAACACCCGGTTTCTCCACTCTGGAGTGTTAATTTGATGGTACTGCCATTCTTGTTTAATGTGATCTCATGCTTAAAAGTTTTGCAACTTTCAATATGCCCTGGAAATGCAAGTTGTGCTTATGTCTGTATATTGCAATGTGATAATCCGCAGAATTTATTACTGAATCCTCCTATCTACATTTTTTGTTCATTTCCTTATTAGGAAAATCTCGTGCTTGATGAATTATTCTACTCTACATACTCAATTGATGAAGTAGCCTCGTGCATTTTGTGGAGGATGATTTTTCTTGTGTTTTGTTGCCAGTCTTTATGGCTGATGGTTGCAATGTATCCCAATATTTTTTGTTGTAGTTTATGATGGCCAAACATTTGTAGCTTTGTTTATTTCAAATCTTCGTTTGAGCTTTCTTTCATTTTTGGATTTTTGTGATTTTCCTTGTTCTTAGCTTTATGTTTTGGATATCACTTTCAAATGGGTTTTAATTTTATATTTCATGTCTACTTTAGTCTGAGCTGCATTTTGGAGTTTATTCTCTCTATTCTGTCTGCCTTTTTGGGGTCTACAGTTTGTTCCTTTACATTCTTCTTGTTGATTTATTTATTTTTTTTAAAATCATTGTTATTTTTGCTGTTCCCTATCCTTCTATCTCTAATTTACCTCGTGTTCTTATCTGCCAACCCATTTGGATCGTTTTGCGTATTGGATATGAATATCAAATATTAGGATTAAATGTCTAGAATATGAAATAGTTCTGTTTATAATTTAAACATTTGTCCTTTCCAAGAATTGTTGTTTTGGAAATGATCTATTTTTGTTATATAGGTTTCCTTCCTGACAACTTAATTAGATTAATCAATTAGATAGATCTGAAAGAACAAATTAATAATATTAAATTGAAAGATTCGATTCATAGTCATTAGAATTTGGTTGCAGCGGCCATACAGTGTTTGAAGAGGAAAAGGCTTTATGAACAGCAAGTAGAGCAACTTGGAAATTTCCAATTGCGTATCCATGATCAGGTGAGCTGGAGTTGTTATTTACTGATTGATGAGACATTTATTTCTTCTACTTTCAGTTAACTGCATTGAACTAAACCTTTTACTTTATGAACAATTTTTTTACTGTTGCAGATGATTATGTTAGAAGGCGCAAAAGCTACAACGGAGACTGTCGACGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACGTATGCCAAATATTTATTTGCTTTGCTCTCTTTCTGCCTTTGTGGGTTATAATCTTTAGGATTTTCTTCTTGCTGGGTTATAGTCATCTTAGATATATAGTTCCAAGGTTTCTATAAAAATTTTAACCAACATTCTTTTTGTGTTTATAATTGATCTTTTAACACAACTATTAACATTACTATTATTTATGCACTTCGTTCATCGAGTTATTGATGTTATGCTAACCAATCTTGAGAAAGTTAGCTATTACCATTAAGCATTACTTGCAATGGCCACTGAAAAATGAAATAATTTGTGAAAGTTTGTTTCATGTTTTGTTTCTTTAACCGAGGTTGTGAAATTTGGAACCGTACTTATTCTTAAAAAACTTTCTTCTCCAGAAACATTGATGATGTGGACAAGACGATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAAGCGCTGTCAACTCCAATTGGTGCTGCAGCTGATTTCGATGAGGTACTTAATCTTTGGCTTGTTTCTTGGCCTTTCCCGGTATTTGCTTATGTCTCATAATTCATCTATTAACAATGGTGGTGTAATCAGGATGAATTGGAGGCAGAACTCGAGGAGCTTGAAAGTGCTGAGTTGGAAGAACAACTTCTCCAGCCAGCCTCAACGGCTCCTGCTGGACCTGTCTCAGTCCTGTCAGGCCGAGTTCCTGCTCGCCCTGTTCCACAGAAGAGAACTGCTGAAGAAGACGAGCTCGCAGCTCTGCAAGCTGAGATGGCCCTCTAACCAGGTTTGTTCCGTAGCCCAATCTATAAGCTGAAGAGCTCCGATTAACAGTAAATGTTTTGATTATTTATTGTTATCTTGTCTTTTGCAGTACTTAATAAACTCGATGAACTAAATTTGTAGTTGAACGTTGTTTTATCTTAAAAAATTACCTTTTATCCATTGTTTGAGCAGAAAACTGAATTTTAATTGGACATATGCAGGTGGTTCATATGAGTATCATCTTCTGGCAGATTGCATCCGAACCTTCTAAGCGTTTGATTGAAATTTTACACAAATTACCCTTCCCTGCGGCAAGACAATGTATATATAAAGGTGCAAAGTTACTGGGATCTTCATCTTAAAATCATCTAAAAATTTTATACGAATCTTCAATTTTAAATTATTGGGAAATGTAGGTAATATTTGAGTAATTATAATTTCTTTGACTTATAATAAAAATGCCATGTTTGTTTA

mRNA sequence

AAATAGATTGCCGCGTGTGGTGGACAAACAGCGACGGGAAGAGAGGGAACTCCAAATCTTCGTCCTTCCTTTTCTGGAGCGCCTTGGAAGAAACCCTTTCTACTTCAAATTTCTTCACAAATTCTTTCATTTCATTCTCGTTGGTATAAATTTGTCTCTGATTATCATCTTTCCGTTTCAATCTCTTGCTGGTTTCTCGATTTCTTTCTGATCGAGGGGCGATTCTTTTTTCTTAGCTTAAACAATGTTAAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGACAAGTTGAACGAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTATTAAGAAGGCTGCTGCAGAGGTTGAAAAAGCCAAAGAATTCACAAGAGTGAAGAATAAACGAGCGGCCATACAGTGTTTGAAGAGGAAAAGGCTTTATGAACAGCAAGTAGAGCAACTTGGAAATTTCCAATTGCGTATCCATGATCAGATGATTATGTTAGAAGGCGCAAAAGCTACAACGGAGACTGTCGACGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACAAACATTGATGATGTGGACAAGACGATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAAGCGCTGTCAACTCCAATTGGTGCTGCAGCTGATTTCGATGAGGATGAATTGGAGGCAGAACTCGAGGAGCTTGAAAGTGCTGAGTTGGAAGAACAACTTCTCCAGCCAGCCTCAACGGCTCCTGCTGGACCTGTCTCAGTCCTGTCAGGCCGAGTTCCTGCTCGCCCTGTTCCACAGAAGAGAACTGCTGAAGAAGACGAGCTCGCAGCTCTGCAAGCTGAGATGGCCCTCTAACCAGGTGGTTCATATGAGTATCATCTTCTGGCAGATTGCATCCGAACCTTCTAAGCGTTTGATTGAAATTTTACACAAATTACCCTTCCCTGCGGCAAGACAATGTATATATAAAGGTGCAAAGTTACTGGGATCTTCATCTTAAAATCATCTAAAAATTTTATACGAATCTTCAATTTTAAATTATTGGGAAATGTAGGTAATATTTGAGTAATTATAATTTCTTTGACTTATAATAAAAATGCCATGTTTGTTTA

Coding sequence (CDS)

ATGTTAAACAGGCTTTTTGGTAAACCTAAACAAGAAGCCAATGCTTTGGCTACGTTAGACAAGTTGAACGAGACACTTGAAATGCTGGAGAAGAAAGAGAACGTTCTTATTAAGAAGGCTGCTGCAGAGGTTGAAAAAGCCAAAGAATTCACAAGAGTGAAGAATAAACGAGCGGCCATACAGTGTTTGAAGAGGAAAAGGCTTTATGAACAGCAAGTAGAGCAACTTGGAAATTTCCAATTGCGTATCCATGATCAGATGATTATGTTAGAAGGCGCAAAAGCTACAACGGAGACTGTCGACGCTTTGAGAACAGGAGCATCTGCAATGAAGGCCATGCAGAAAGCAACAAACATTGATGATGTGGACAAGACGATGGATGAGATCAATGAGCAGACTGAGAATATGAAACAGATACAGGAAGCGCTGTCAACTCCAATTGGTGCTGCAGCTGATTTCGATGAGGATGAATTGGAGGCAGAACTCGAGGAGCTTGAAAGTGCTGAGTTGGAAGAACAACTTCTCCAGCCAGCCTCAACGGCTCCTGCTGGACCTGTCTCAGTCCTGTCAGGCCGAGTTCCTGCTCGCCCTGTTCCACAGAAGAGAACTGCTGAAGAAGACGAGCTCGCAGCTCTGCAAGCTGAGATGGCCCTCTAA

Protein sequence

MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTAPAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL
Homology
BLAST of CmaCh12G012850 vs. ExPASy Swiss-Prot
Match: Q9SZE4 (Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana OX=3702 GN=VPS32.2 PE=1 SV=1)

HSP 1 Score: 346.7 bits (888), Expect = 2.0e-94
Identity = 194/219 (88.58%), Postives = 205/219 (93.61%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M+NRLFGKPKQEANAL TLDKLNETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAGP-VSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           AP  P V V +GR PARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of CmaCh12G012850 vs. ExPASy Swiss-Prot
Match: O82197 (Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana OX=3702 GN=VPS32.1 PE=1 SV=1)

HSP 1 Score: 317.8 bits (813), Expect = 9.7e-86
Identity = 184/218 (84.40%), Postives = 191/218 (87.61%), Query Frame = 0

Query: 2   LNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQ 61
           +NRLFGKPKQE + L TLDKLNETLEMLEKKENVL+KKA  EVEKAKEF+R KNKRAAIQ
Sbjct: 3   MNRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQ 62

Query: 62  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 121
           CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD
Sbjct: 63  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 122

Query: 122 VDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTA 181
           VDKTMDEINEQTENMKQIQEALS P G A DFDEDELEAEL+ELE AELEEQLLQP    
Sbjct: 123 VDKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQPV--- 182

Query: 182 PAGPVSVLSGRVPARPVPQKR-TAEEDELAALQAEMAL 219
              P+ V  G  PAR   QK+ TAEEDELAALQAEMAL
Sbjct: 183 ---PIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of CmaCh12G012850 vs. ExPASy Swiss-Prot
Match: Q7ZVC4 (Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 120.9 bits (302), Expect = 1.7e-26
Identity = 86/208 (41.35%), Postives = 120/208 (57.69%), Query Frame = 0

Query: 7   GKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRK 66
           GK  + A+    + +L ET EML KK+  L KK   E+  AK+    KNKRAA+Q LKRK
Sbjct: 12  GKGGKSASPQEAIQRLRETEEMLTKKQEFLEKKIEQELVTAKK-NGTKNKRAALQALKRK 71

Query: 67  RLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTM 126
           + YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     +ID VD+ M
Sbjct: 72  KRYEKQLAQIDGTLSTIEFQREALENAHTNTEVIKNMGYAAKAMKAAHDNMDIDKVDELM 131

Query: 127 DEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTAPAGPV 186
            +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LL+     P    
Sbjct: 132 QDIIEQQELAQEISDAISKPVGFGEEFDEDELLAELEELEQEELDKNLLEIGDNVPL--P 191

Query: 187 SVLSGRVPARPVPQKRTAEEDELAALQA 215
           +V S  +P+RP  +K   +ED++  L+A
Sbjct: 192 NVPSTSLPSRPAKKKEEEDEDDMKDLEA 216

BLAST of CmaCh12G012850 vs. ExPASy Swiss-Prot
Match: Q6IQ73 (Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=1)

HSP 1 Score: 118.2 bits (295), Expect = 1.1e-25
Identity = 89/208 (42.79%), Postives = 117/208 (56.25%), Query Frame = 0

Query: 7   GKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRK 66
           GK  +       + KL ET EML KK++ L KK  AE+  AK+    KNKRAA+Q LKRK
Sbjct: 12  GKGGKGPTPQEAIQKLRETEEMLAKKQDFLEKKIDAELLIAKK-NGTKNKRAALQALKRK 71

Query: 67  RLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTM 126
           + YE+Q+ Q+      I  Q   LE A   TE +  +   A AMK   +  +ID VD  M
Sbjct: 72  KRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKTAHENMDIDKVDDLM 131

Query: 127 DEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTAPAGPV 186
            +I EQ E  ++I +A+S P+G   +FDEDEL AELEELE  EL++ LLQ      +GP 
Sbjct: 132 QDITEQQELAQEISDAISRPVGFGEEFDEDELMAELEELEQEELDKDLLQ-----ISGPE 191

Query: 187 SVLSGRVPARPVP------QKRTAEEDE 209
            V    VP+ P+P      QK+  EEDE
Sbjct: 192 DVPLPNVPSNPLPKKTAVAQKKRQEEDE 213

BLAST of CmaCh12G012850 vs. ExPASy Swiss-Prot
Match: Q5XGW6 (Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 SV=1)

HSP 1 Score: 117.1 bits (292), Expect = 2.5e-25
Identity = 83/216 (38.43%), Postives = 124/216 (57.41%), Query Frame = 0

Query: 1   MLNRLF---GKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKR 60
           ++ +LF   GK  +  +    + KL +T EML KK+  L KK   E+  AK+    KNKR
Sbjct: 3   LIGKLFGTGGKGAKGPSPQEAIQKLRDTEEMLAKKQEFLEKKIEQELVTAKKH-GTKNKR 62

Query: 61  AAIQCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKAT 120
           AA+Q LKRK+ YE+Q+ Q+      I  Q   LE A   TE +  +   A AMKA     
Sbjct: 63  AALQALKRKKRYEKQLAQIDGTLSTIEFQREALENANTNTEVLKNMGFAAKAMKAAHDNM 122

Query: 121 NIDDVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQP 180
           +I+ VD+ M +I +Q E  ++I +A+S P+G   DFDEDEL AELEELE  EL++ LL+ 
Sbjct: 123 DIEKVDELMQDIADQQELAQEISDAISKPVGFGEDFDEDELMAELEELEQEELDKNLLEV 182

Query: 181 ASTAPAGPVSVLSGRVPARPVPQKRTAEEDELAALQ 214
                    +V +  +PA+PV +K+  ++D++  L+
Sbjct: 183 QGPETVPLPNVPAASLPAKPVKKKQEEDDDDMRELE 217

BLAST of CmaCh12G012850 vs. ExPASy TrEMBL
Match: A0A6J1HQ32 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita maxima OX=3661 GN=LOC111465657 PE=4 SV=1)

HSP 1 Score: 393.3 bits (1009), Expect = 6.7e-106
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of CmaCh12G012850 vs. ExPASy TrEMBL
Match: A0A6J1FBK1 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata OX=3662 GN=LOC111444128 PE=4 SV=1)

HSP 1 Score: 390.6 bits (1002), Expect = 4.4e-105
Identity = 217/218 (99.54%), Postives = 217/218 (99.54%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           APAGPVSV SGRVPARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of CmaCh12G012850 vs. ExPASy TrEMBL
Match: A0A6J1G2S3 (vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita moschata OX=3662 GN=LOC111450291 PE=4 SV=1)

HSP 1 Score: 377.1 bits (967), Expect = 5.0e-101
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMD+INEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            PA PVS+ SGRVPARPVPQKRTAEEDEL ALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELTALQAEMAL 218

BLAST of CmaCh12G012850 vs. ExPASy TrEMBL
Match: A0A1S3CAQ7 (vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656 GN=LOC103498351 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.5e-100
Identity = 206/218 (94.50%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKAAAEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           APA PVSV +GRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of CmaCh12G012850 vs. ExPASy TrEMBL
Match: A0A5A7V4V7 (Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00800 PE=4 SV=1)

HSP 1 Score: 375.6 bits (963), Expect = 1.5e-100
Identity = 206/218 (94.50%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKAAAEVE+AKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKAAAEVERAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQ+EQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQIEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALS PIGAAADFDEDELEAELE+LESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSAPIGAAADFDEDELEAELEDLESAELEEQLLQPAST 180

Query: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           APA PVSV +GRVPARP PQKRTAEEDELAALQAEMAL
Sbjct: 181 APAAPVSVPAGRVPARPAPQKRTAEEDELAALQAEMAL 218

BLAST of CmaCh12G012850 vs. NCBI nr
Match: XP_022965914.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita maxima] >XP_022965915.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita maxima] >XP_022965916.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita maxima] >XP_022965917.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita maxima])

HSP 1 Score: 393.3 bits (1009), Expect = 1.4e-105
Identity = 218/218 (100.00%), Postives = 218/218 (100.00%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of CmaCh12G012850 vs. NCBI nr
Match: XP_022937864.1 (vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937865.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937866.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_022937867.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >XP_023536810.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >XP_023536811.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >XP_023536812.1 vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita pepo subsp. pepo] >KAG7021320.1 Vacuolar protein sorting-associated protein 32-like 2, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 390.6 bits (1002), Expect = 9.0e-105
Identity = 217/218 (99.54%), Postives = 217/218 (99.54%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI
Sbjct: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           APAGPVSV SGRVPARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 APAGPVSVPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of CmaCh12G012850 vs. NCBI nr
Match: KAG7030473.1 (Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 379.8 bits (974), Expect = 1.6e-101
Identity = 209/218 (95.87%), Postives = 214/218 (98.17%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            PA PVS+ SGRVPARPVPQKRTAEEDELAALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELAALQAEMAL 218

BLAST of CmaCh12G012850 vs. NCBI nr
Match: XP_023545615.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545616.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545619.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023545620.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547008.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547009.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547010.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo] >XP_023547011.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 378.3 bits (970), Expect = 4.6e-101
Identity = 209/218 (95.87%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            PA PVSV SGRVPARPVPQKR AEEDELAALQAEMAL
Sbjct: 181 TPAAPVSVPSGRVPARPVPQKRNAEEDELAALQAEMAL 218

BLAST of CmaCh12G012850 vs. NCBI nr
Match: XP_022946105.1 (vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946106.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946107.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata] >XP_022946108.1 vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschata])

HSP 1 Score: 377.1 bits (967), Expect = 1.0e-100
Identity = 207/218 (94.95%), Postives = 213/218 (97.71%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M NRLFGKPKQEANALATL+KL+ETLEMLEKKENVL+KKA+AEVEKAKEFTRVKNKRAAI
Sbjct: 1   MFNRLFGKPKQEANALATLEKLSETLEMLEKKENVLVKKASAEVEKAKEFTRVKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMD+INEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST
Sbjct: 121 DVDKTMDDINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180

Query: 181 APAGPVSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
            PA PVS+ SGRVPARPVPQKRTAEEDEL ALQAEMAL
Sbjct: 181 TPAAPVSIPSGRVPARPVPQKRTAEEDELTALQAEMAL 218

BLAST of CmaCh12G012850 vs. TAIR 10
Match: AT4G29160.1 (SNF7 family protein )

HSP 1 Score: 346.7 bits (888), Expect = 1.4e-95
Identity = 194/219 (88.58%), Postives = 205/219 (93.61%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M+NRLFGKPKQEANAL TLDKLNETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAGP-VSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           AP  P V V +GR PARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of CmaCh12G012850 vs. TAIR 10
Match: AT4G29160.3 (SNF7 family protein )

HSP 1 Score: 346.7 bits (888), Expect = 1.4e-95
Identity = 194/219 (88.58%), Postives = 205/219 (93.61%), Query Frame = 0

Query: 1   MLNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAI 60
           M+NRLFGKPKQEANAL TLDKLNETLEMLEKKE VL+KKA AEVEKAKE++R KNKRAAI
Sbjct: 1   MMNRLFGKPKQEANALQTLDKLNETLEMLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAI 60

Query: 61  QCLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNID 120
           QCLKRKRLYE QVEQLGNFQLRIHDQMIMLEGAKATTETVDALR+GASAMKAMQKATNID
Sbjct: 61  QCLKRKRLYEGQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRSGASAMKAMQKATNID 120

Query: 121 DVDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPAST 180
           DVDKTMDEINEQTENMKQIQEAL+TP+GAAADFDEDEL AEL+ELES ELE QLLQPA+T
Sbjct: 121 DVDKTMDEINEQTENMKQIQEALATPMGAAADFDEDELAAELDELESEELESQLLQPATT 180

Query: 181 APAGP-VSVLSGRVPARPVPQKRTAEEDELAALQAEMAL 219
           AP  P V V +GR PARPVPQKRTAEE+ELAALQAEMAL
Sbjct: 181 APPLPSVPVPAGRQPARPVPQKRTAEEEELAALQAEMAL 219

BLAST of CmaCh12G012850 vs. TAIR 10
Match: AT2G19830.1 (SNF7 family protein )

HSP 1 Score: 317.8 bits (813), Expect = 6.9e-87
Identity = 184/218 (84.40%), Postives = 191/218 (87.61%), Query Frame = 0

Query: 2   LNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQ 61
           +NRLFGKPKQE + L TLDKLNETLEMLEKKENVL+KKA  EVEKAKEF+R KNKRAAIQ
Sbjct: 3   MNRLFGKPKQETSTLQTLDKLNETLEMLEKKENVLLKKATGEVEKAKEFSRAKNKRAAIQ 62

Query: 62  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 121
           CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD
Sbjct: 63  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 122

Query: 122 VDKTMDEINEQTENMKQIQEALSTPIGAAADFDEDELEAELEELESAELEEQLLQPASTA 181
           VDKTMDEINEQTENMKQIQEALS P G A DFDEDELEAEL+ELE AELEEQLLQP    
Sbjct: 123 VDKTMDEINEQTENMKQIQEALSAPFG-ANDFDEDELEAELDELEGAELEEQLLQPV--- 182

Query: 182 PAGPVSVLSGRVPARPVPQKR-TAEEDELAALQAEMAL 219
              P+ V  G  PAR   QK+ TAEEDELAALQAEMAL
Sbjct: 183 ---PIHVPQGNKPARAPAQKQPTAEEDELAALQAEMAL 213

BLAST of CmaCh12G012850 vs. TAIR 10
Match: AT4G29160.2 (SNF7 family protein )

HSP 1 Score: 298.9 bits (764), Expect = 3.3e-81
Identity = 169/192 (88.02%), Postives = 179/192 (93.23%), Query Frame = 0

Query: 28  MLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQCLKRKRLYEQQVEQLGNFQLRIHDQM 87
           MLEKKE VL+KKA AEVEKAKE++R KNKRAAIQCLKRKRLYE QVEQLGNFQLRIHDQM
Sbjct: 1   MLEKKEKVLLKKAGAEVEKAKEYSRAKNKRAAIQCLKRKRLYEGQVEQLGNFQLRIHDQM 60

Query: 88  IMLEGAKATTETVDALRTGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALSTPI 147
           IMLEGAKATTETVDALR+GASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEAL+TP+
Sbjct: 61  IMLEGAKATTETVDALRSGASAMKAMQKATNIDDVDKTMDEINEQTENMKQIQEALATPM 120

Query: 148 GAAADFDEDELEAELEELESAELEEQLLQPASTAPAGP-VSVLSGRVPARPVPQKRTAEE 207
           GAAADFDEDEL AEL+ELES ELE QLLQPA+TAP  P V V +GR PARPVPQKRTAEE
Sbjct: 121 GAAADFDEDELAAELDELESEELESQLLQPATTAPPLPSVPVPAGRQPARPVPQKRTAEE 180

Query: 208 DELAALQAEMAL 219
           +ELAALQAEMAL
Sbjct: 181 EELAALQAEMAL 192

BLAST of CmaCh12G012850 vs. TAIR 10
Match: AT5G09260.1 (vacuolar protein sorting-associated protein 20.2 )

HSP 1 Score: 55.5 bits (132), Expect = 6.4e-08
Identity = 58/206 (28.16%), Postives = 96/206 (46.60%), Query Frame = 0

Query: 2   LNRLFGKPKQEANALATLDKLNETLEMLEKKENVLIKKAAAEVEKAKEFTRVKNKRAAIQ 61
           +  LF K  +       +  L      L + +  L K   AE + A++  R K K  A+ 
Sbjct: 1   MGNLFVKKPKITEVDRAILSLKTQRRKLGQYQQQLEKVIEAEKQAARDLIREKRKDRALL 60

Query: 62  CLKRKRLYEQQVEQLGNFQLRIHDQMIMLEGAKATTETVDALRTGASAMKAMQKATNIDD 121
            LK+KR  E+ ++Q+  + + +  Q+  +E         ++L+ G +A+KA+Q   N+DD
Sbjct: 61  ALKKKRTQEELLKQVDQWLINVEQQLADIELTSKQKAVFESLKQGNNAIKAIQSEVNLDD 120

Query: 122 VDKTMDEINEQTENMKQIQEALSTPIGAAADF-DEDELEAELEELESAELEEQLLQPAST 181
           V K MD+    T   K  Q+ LS  +G      DE+E+ AE + LES  + E + +  +T
Sbjct: 121 VQKLMDD----TAEAKAYQDELSAILGEKLSAEDEEEILAEFDNLESLLIVEDMPEVPTT 180

Query: 182 --APAGPVSVLSGRVPAR-PVPQKRT 204
              P  P  +    VP + PV    T
Sbjct: 181 ELMPEEPEKMDLPDVPTKAPVASNET 202

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SZE42.0e-9488.58Vacuolar protein sorting-associated protein 32 homolog 2 OS=Arabidopsis thaliana... [more]
O821979.7e-8684.40Vacuolar protein sorting-associated protein 32 homolog 1 OS=Arabidopsis thaliana... [more]
Q7ZVC41.7e-2641.35Charged multivesicular body protein 4b OS=Danio rerio OX=7955 GN=chmp4b PE=2 SV=... [more]
Q6IQ731.1e-2542.79Charged multivesicular body protein 4c OS=Danio rerio OX=7955 GN=chmp4c PE=2 SV=... [more]
Q5XGW62.5e-2538.43Charged multivesicular body protein 4b OS=Xenopus laevis OX=8355 GN=chmp4b PE=2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1HQ326.7e-106100.00vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita maxima OX=... [more]
A0A6J1FBK14.4e-10599.54vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucurbita moschata O... [more]
A0A6J1G2S35.0e-10194.95vacuolar protein sorting-associated protein 32 homolog 2-like OS=Cucurbita mosch... [more]
A0A1S3CAQ71.5e-10094.50vacuolar protein sorting-associated protein 32 homolog 2 OS=Cucumis melo OX=3656... [more]
A0A5A7V4V71.5e-10094.50Vacuolar protein sorting-associated protein 32-like protein 2 OS=Cucumis melo va... [more]
Match NameE-valueIdentityDescription
XP_022965914.11.4e-105100.00vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita maxima] >XP_... [more]
XP_022937864.19.0e-10599.54vacuolar protein sorting-associated protein 32 homolog 2 [Cucurbita moschata] >X... [more]
KAG7030473.11.6e-10195.87Vacuolar protein sorting-associated protein 32-like 2 [Cucurbita argyrosperma su... [more]
XP_023545615.14.6e-10195.87vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita pepo su... [more]
XP_022946105.11.0e-10094.95vacuolar protein sorting-associated protein 32 homolog 2-like [Cucurbita moschat... [more]
Match NameE-valueIdentityDescription
AT4G29160.11.4e-9588.58SNF7 family protein [more]
AT4G29160.31.4e-9588.58SNF7 family protein [more]
AT2G19830.16.9e-8784.40SNF7 family protein [more]
AT4G29160.23.3e-8188.02SNF7 family protein [more]
AT5G09260.16.4e-0828.16vacuolar protein sorting-associated protein 20.2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 12..39
NoneNo IPR availableCOILSCoilCoilcoord: 122..142
NoneNo IPR availableCOILSCoilCoilcoord: 151..175
NoneNo IPR availableGENE3D1.10.287.1060coord: 14..91
e-value: 2.4E-22
score: 80.8
NoneNo IPR availableGENE3D6.10.250.1710coord: 117..171
e-value: 3.0E-9
score: 38.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 173..218
NoneNo IPR availablePANTHERPTHR22761:SF71SNF7 FAMILY-RELATEDcoord: 1..218
NoneNo IPR availablePANTHERPTHR22761CHARGED MULTIVESICULAR BODY PROTEINcoord: 1..218
IPR005024Snf7 familyPFAMPF03357Snf7coord: 20..185
e-value: 1.4E-45
score: 155.0

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G012850.1CmaCh12G012850.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032511 late endosome to vacuole transport via multivesicular body sorting pathway
biological_process GO:0006900 vesicle budding from membrane
biological_process GO:0007034 vacuolar transport
cellular_component GO:0009898 cytoplasmic side of plasma membrane
cellular_component GO:0000815 ESCRT III complex
cellular_component GO:0005771 multivesicular body