CmaCh12G012640 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G012640
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionninja-family protein mc410-like
LocationCma_Chr12: 9849132 .. 9851676 (-)
RNA-Seq ExpressionCmaCh12G012640
SyntenyCmaCh12G012640
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
TCTTACAGTTGCAGATTGCCGAAAATTCCCACGGGTTCTTCGCTTTACAAGATGCCATAGCTCAAGCTTTAGGGTTCGGTTTCAGGTTTTCTTCTAGCGTGAATTTTATTCGGTATGTGCCTCTGTTAAATTTCTTCTATTATGTTTAAAGATTTCGAGTACTTTCGGAGTTCGTTTCGATTTAGTCGCGTTGGAGTTCTTGACTTGAACTAGGATCGTGTTTAGATTGAGTTGAATTCTGTTTGATTTTCTTGTTGTTTCTTTGGATTTTAAGATTTTAATAAACTTCATCTGTGTGGATACCATCGGGCCAATTTGGAGATGTAGGAATCGTTACAGACATAGCTATATTTTTAAGATAGGTGAGTCATCGGGCCAATCCTTGTATTATGCTCATGGTATACAACAAGAAGTTATGTTGTTTTCAATTTGGAGCTTGAGCTCAGACCTTAAATGGATATCTCCAAACATTTCAAACTTTTATCACGAGCACACCATTGGGGTTATTGGAATGTTTGTGATGATGCATTTGTGAGATCCCAAATCGGCTGGAGAGGGGAACGAAACGTTTCTTGTAAGGGTGTGGAACTTCTCCCTAATAGACGCGTTTTAAAACCATGAGGCTGACGGCAATACGTAACGGGCCAAAGCGGATAATAGCTGCTAGCAATGGGTTTGAGCTATTACAAATGATATTAGAGCTAGACATCGGCAGTGTACCAGCAAGGACTCTGGGTTCCCAAGTGGGTGGAGTGTGAGATCCCACATCGATTGGAGAGGGAAACGAAGCATTCCTTACAAGGGTGTGGAAACTTCTCCCTAACAAACATGTTTTGAAACTATTAGGCTGACGGCGATACGTAGTAGGTCAAAGCGAACCATATCTGCTAACGGTAGGCTTAGCATTAGGCTGAATCTCGTGAGTTAATTATTGCTGTTATATATTGCAGGTAAGGAATAAGTGTATGTCTTTTGCTAATGTCAATGAACTTATAGTTTGAAAGATGGAGAATGAGGATGGGCTTGAGCTTAGCTTGGGTCTGACCCTTGGTGGAGCTTGTGTCAAGTCTAGAAGTAAACTTACGAGCTCCTCCGGAGCAAACGCCGAGGAAAGCGATAGAGGCAATAAATTGGTCGATGATTTTAAGAATTTTCGACATGGAGATAATCCGAGGCAGGAATCAGACACGGGATCTTTTGAAAGTAATTCAATTCCATCTAAAGATAATTTCTTCAATGACCTGTTGAAGGTCGGAATGGATGGAGATTCTTTGATCGATTTGAAGAGGAAAGGAACCTGGATCGAAAGTAGTTATGCAGAAGCCGAGGATGAAAATCTGCCAGATATCAGCAATAAACGGAAGTTGTTGTTCGTGGAAATGAACGGTCAAAAGAAGCGCGAAAGAGAATCTCACGAAAAAGGAATGGCATCACACATTTCCTCTACTGAAGATGGTTCGACTGCCGAAAAAGGCGATGTCGTAGAGTCTAGACCCGAAGGTTCGACATCAAGGTTAGCATCAAAACATGATGATAGCTACCAACAATGTATATCTGTGGAATTTCTGTGGAATTTGCCTAAAGTTTCAAAGGATATCTGTGGAATTTCTGATTCAAGTAGGCAGAAAAGGTTTAACAGTTCATCAGTTGGAGTTCCAAACTCTATCCAAGTCATGAATATGCATAATGTTAACACTCATGGTGTGCCTAGCTCAATGGGTCACTTGCAACTCGGAATGCAGCATGTGCCACCTACTATAAACGGTGACCGATCGGGACCCGAGCATGTAAACCTTAGAAACTTGCCTCTTACAGCTGGTGTTTCACCGCTTCCGATTTCGGCAATGGACAAAGATAAGCCATGGGGTTTGGTTTCTCATCCTCAAATGATCCATCGCCCCTATGGCGGGGGCACCCCGTCTACTAGCTCAGGTAGACATGATTTCGTACCAAAAAAACTCGGTTTTGTTTGCATCTTTCAAGTTTTTAAGAAGAGAGCCTGATAGATAGTTACTTATGCTGCCTGTCTGCATATTTACGTGTAGTTGCCGAGCAAGTAATCGGACATTTTTCATTCGAGGGATTGCTCCACGGTGAGAGCGCAACGGAACGTACCAAAGACGATAGCAAACAGTCCGCCATGGAAGAGGGTTTGTCCTCTTCTCGAGCAGAACACATGAAAGTAAGCATTCCGAACGTTAATACGAAAGACATATTAGAGCGACCGAAAGCGGCTGGTGTGTCCCTTGATATCCCAGCTATAAAGCCAGGCATTGGTACAGATATAGAATTTGGGGGATGTGGTTCATACCCAAACCTCCCTTGGGTTTCCACCACTGGTGCTGGCCCTACTGGTAAGACTATCTCTGGTGTAACATATAGGTTTAATGCAAACCAGATCAAGATTGTTTGTGCTTGCCATGGTACTCACATGTCTCCTGAAGAGTTCATTCGACATGCGAACGGCGAGAATTTTAGTGCACAGAATGACAATGGTCCCGCAACGATTCCTAACAAAAACCCCACTGCCTCGGCACGAAGTTGA

mRNA sequence

TCTTACAGTTGCAGATTGCCGAAAATTCCCACGGGTTCTTCGCTTTACAAGATGCCATAGCTCAAGCTTTAGGGTTCGGTTTCAGGTTTTCTTCTAGCGTGAATTTTATTCGGTAAGGAATAAGTGTATGTCTTTTGCTAATGTCAATGAACTTATAGTTTGAAAGATGGAGAATGAGGATGGGCTTGAGCTTAGCTTGGGTCTGACCCTTGGTGGAGCTTGTGTCAAGTCTAGAAGTAAACTTACGAGCTCCTCCGGAGCAAACGCCGAGGAAAGCGATAGAGGCAATAAATTGGTCGATGATTTTAAGAATTTTCGACATGGAGATAATCCGAGGCAGGAATCAGACACGGGATCTTTTGAAAGTAATTCAATTCCATCTAAAGATAATTTCTTCAATGACCTGTTGAAGGTCGGAATGGATGGAGATTCTTTGATCGATTTGAAGAGGAAAGGAACCTGGATCGAAAGTAGTTATGCAGAAGCCGAGGATGAAAATCTGCCAGATATCAGCAATAAACGGAAGTTGTTGTTCGTGGAAATGAACGGTCAAAAGAAGCGCGAAAGAGAATCTCACGAAAAAGGAATGGCATCACACATTTCCTCTACTGAAGATGGTTCGACTGCCGAAAAAGGCGATGTCGTAGAGTCTAGACCCGAAGGTTCGACATCAAGGTTAGCATCAAAACATGATGATAGCTACCAACAATGTATATCTGTGGAATTTCTGTGGAATTTGCCTAAAGTTTCAAAGGATATCTGTGGAATTTCTGATTCAAGTAGGCAGAAAAGGTTTAACAGTTCATCAGTTGGAGTTCCAAACTCTATCCAAGTCATGAATATGCATAATGTTAACACTCATGGTGTGCCTAGCTCAATGGGTCACTTGCAACTCGGAATGCAGCATGTGCCACCTACTATAAACGGTGACCGATCGGGACCCGAGCATGTAAACCTTAGAAACTTGCCTCTTACAGCTGGTGTTTCACCGCTTCCGATTTCGGCAATGGACAAAGATAAGCCATGGGGTTTGGTTTCTCATCCTCAAATGATCCATCGCCCCTATGGCGGGGGCACCCCGTCTACTAGCTCAGTTGCCGAGCAAGTAATCGGACATTTTTCATTCGAGGGATTGCTCCACGGTGAGAGCGCAACGGAACGTACCAAAGACGATAGCAAACAGTCCGCCATGGAAGAGGGTTTGTCCTCTTCTCGAGCAGAACACATGAAAGTAAGCATTCCGAACGTTAATACGAAAGACATATTAGAGCGACCGAAAGCGGCTGGTGTGTCCCTTGATATCCCAGCTATAAAGCCAGGCATTGGTACAGATATAGAATTTGGGGGATGTGGTTCATACCCAAACCTCCCTTGGGTTTCCACCACTGGTGCTGGCCCTACTGGTAAGACTATCTCTGGTGTAACATATAGGTTTAATGCAAACCAGATCAAGATTGTTTGTGCTTGCCATGGTACTCACATGTCTCCTGAAGAGTTCATTCGACATGCGAACGGCGAGAATTTTAGTGCACAGAATGACAATGGTCCCGCAACGATTCCTAACAAAAACCCCACTGCCTCGGCACGAAGTTGA

Coding sequence (CDS)

ATGGAGAATGAGGATGGGCTTGAGCTTAGCTTGGGTCTGACCCTTGGTGGAGCTTGTGTCAAGTCTAGAAGTAAACTTACGAGCTCCTCCGGAGCAAACGCCGAGGAAAGCGATAGAGGCAATAAATTGGTCGATGATTTTAAGAATTTTCGACATGGAGATAATCCGAGGCAGGAATCAGACACGGGATCTTTTGAAAGTAATTCAATTCCATCTAAAGATAATTTCTTCAATGACCTGTTGAAGGTCGGAATGGATGGAGATTCTTTGATCGATTTGAAGAGGAAAGGAACCTGGATCGAAAGTAGTTATGCAGAAGCCGAGGATGAAAATCTGCCAGATATCAGCAATAAACGGAAGTTGTTGTTCGTGGAAATGAACGGTCAAAAGAAGCGCGAAAGAGAATCTCACGAAAAAGGAATGGCATCACACATTTCCTCTACTGAAGATGGTTCGACTGCCGAAAAAGGCGATGTCGTAGAGTCTAGACCCGAAGGTTCGACATCAAGGTTAGCATCAAAACATGATGATAGCTACCAACAATGTATATCTGTGGAATTTCTGTGGAATTTGCCTAAAGTTTCAAAGGATATCTGTGGAATTTCTGATTCAAGTAGGCAGAAAAGGTTTAACAGTTCATCAGTTGGAGTTCCAAACTCTATCCAAGTCATGAATATGCATAATGTTAACACTCATGGTGTGCCTAGCTCAATGGGTCACTTGCAACTCGGAATGCAGCATGTGCCACCTACTATAAACGGTGACCGATCGGGACCCGAGCATGTAAACCTTAGAAACTTGCCTCTTACAGCTGGTGTTTCACCGCTTCCGATTTCGGCAATGGACAAAGATAAGCCATGGGGTTTGGTTTCTCATCCTCAAATGATCCATCGCCCCTATGGCGGGGGCACCCCGTCTACTAGCTCAGTTGCCGAGCAAGTAATCGGACATTTTTCATTCGAGGGATTGCTCCACGGTGAGAGCGCAACGGAACGTACCAAAGACGATAGCAAACAGTCCGCCATGGAAGAGGGTTTGTCCTCTTCTCGAGCAGAACACATGAAAGTAAGCATTCCGAACGTTAATACGAAAGACATATTAGAGCGACCGAAAGCGGCTGGTGTGTCCCTTGATATCCCAGCTATAAAGCCAGGCATTGGTACAGATATAGAATTTGGGGGATGTGGTTCATACCCAAACCTCCCTTGGGTTTCCACCACTGGTGCTGGCCCTACTGGTAAGACTATCTCTGGTGTAACATATAGGTTTAATGCAAACCAGATCAAGATTGTTTGTGCTTGCCATGGTACTCACATGTCTCCTGAAGAGTTCATTCGACATGCGAACGGCGAGAATTTTAGTGCACAGAATGACAATGGTCCCGCAACGATTCCTAACAAAAACCCCACTGCCTCGGCACGAAGTTGA

Protein sequence

MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQESDTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRKLLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQQCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGHLQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPYGGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS
Homology
BLAST of CmaCh12G012640 vs. ExPASy Swiss-Prot
Match: Q53HY2 (Ninja-family protein mc410 OS=Nicotiana tabacum OX=4097 GN=MC410 PE=2 SV=1)

HSP 1 Score: 285.8 bits (730), Expect = 8.9e-76
Identity = 193/513 (37.62%), Postives = 277/513 (54.00%), Query Frame = 0

Query: 3   NEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQESDT 62
           +E+GL+LSLGL  GG     +SK  SSS +  EE DR  K+++DFKNF  G    Q+ D+
Sbjct: 2   DENGLDLSLGLPCGGGAASEKSKSGSSSDSKVEEVDRDGKVINDFKNFLDGGTSSQKHDS 61

Query: 63  GSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWI--ESSYAEAEDENLPDISNKRK 122
           G     S  +K    N L  + +D D+   L   G W+  +S   E E++   D+ +KRK
Sbjct: 62  GVGSQRSDSTKHE-GNLLSSINVDVDASKKLNSGGFWVQNDSRPVEVEEDRRADVGDKRK 121

Query: 123 LLFVEMNGQKKRERE-----SHEKGMASHIS-STEDGSTAEKGDVVESRPEGSTSRLASK 182
            LF E + QKK+ERE     +H+K   SHIS +T++GSTAE  DV +S   GSTSR   +
Sbjct: 122 NLFRESSQQKKQEREGHHADTHDKTRTSHISITTDEGSTAENEDVADSETVGSTSRQILQ 181

Query: 183 HDDSYQQCISVEFLWNLPKVSKDICGISDSS-----RQKRFNSSSV------GVP----- 242
           HD+S ++ +       L +V K++  +  SS      Q+RF  SS        +P     
Sbjct: 182 HDESSKRFVGSS---GLAEVHKELRSVPASSGVELIGQRRFTISSEKDVKFGNIPYTIPF 241

Query: 243 --NSIQVMNMH-----NVNTHGVPSSMGHLQLGMQHVPPTINGDRSGPEHVNLRNLPLTA 302
              SI +MN+      N NT    S+  +   G+  +  T   DR     V    LPL  
Sbjct: 242 QGQSINIMNLPYSMPLNSNTVSTTSTTSYPVPGVMQLMATTCVDRPPSHPVIPAYLPLMF 301

Query: 303 GVSPLPISAMDKDKPWGLVSHPQMIHRPYGGGT---------PSTSSVAEQVIGHFSFEG 362
           G S + +  +D D   G+ SH   +H  +G G          P+ S  A   I H S + 
Sbjct: 302 GYSSVQLPTLDNDNLHGVASHLLQLHPSHGRGPLGSDKQKDGPNISQAAASSIPHKSSDS 361

Query: 363 LLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAI 422
           + +   A E  K + +Q   EE  +S   E++K S  +   KD  ++P+A  V  +   I
Sbjct: 362 VQYDGRAMEHVKGNGRQHKAEETSNSRGEENVKGSNISFRAKDPPDQPRAEAVPSEFSTI 421

Query: 423 KPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPE 476
           +PG+  D++FGG GSYPNLPWVSTTG GP G+TISGVTYR+++ QI+IVCACHG+HMSP+
Sbjct: 422 RPGLAADLKFGGSGSYPNLPWVSTTGPGPNGRTISGVTYRYSSTQIRIVCACHGSHMSPD 481

BLAST of CmaCh12G012640 vs. ExPASy Swiss-Prot
Match: Q9SV55 (AFP homolog 2 OS=Arabidopsis thaliana OX=3702 GN=AFPH2 PE=1 SV=1)

HSP 1 Score: 233.4 bits (594), Expect = 5.3e-60
Identity = 177/483 (36.65%), Postives = 249/483 (51.55%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGAN-----AEESDRGNKLVDDFKNFRHGDN 60
           M++++GLELSLGL+ GG+  K++    +++G++     AE  DR  K++DDFKNF H  +
Sbjct: 1   MDDDNGLELSLGLSCGGSTGKAKGNNNNNAGSSSENYRAEGGDRSAKVIDDFKNFLHPTS 60

Query: 61  PR-QESDTGSFESNS-IPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLP 120
            R  E  +GS  S+S      NFFNDL K      +  +   K  W+       EDE+  
Sbjct: 61  QRPAEPSSGSQRSDSGQQPPQNFFNDLSKA---PTTEAEASTKPLWV-------EDESRK 120

Query: 121 DISNKRKLLFVEMNGQKKRERES-----HE-KGMASHIS-STEDGSTAEKGDVVESRPEG 180
           +  NKRK  F  MN  KK+E++S     HE K  ASH+S +T++GSTAE  DV ES   G
Sbjct: 121 EAGNKRKFGFPGMNDDKKKEKDSSHVDMHEKKTKASHVSTATDEGSTAENEDVAESEVGG 180

Query: 181 STSRLASKHDDSYQQCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNM 240
            +S   +K                + +   D   + + + Q+R N    G     +   M
Sbjct: 181 GSSSNHAK---------------EVVRPPTDTNIVDNLTGQRRSNHGGSGT----EEFTM 240

Query: 241 HNVNTHGVPSSMGHLQLGMQHVPPTINGDRSGPE---------HVNLRNLPLTAGVSPLP 300
            N+ ++ VP ++ H Q  +  +P ++    SG           + N  NLP+  G SP+ 
Sbjct: 241 RNM-SYTVPFTV-HPQNVVTSMPYSLPTKESGQHAAATSLLQPNANAGNLPIMFGYSPVQ 300

Query: 301 ISAMDKDKPWGLVSHPQMIHRPYGGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDS 360
           +  +DKD   G+V+  Q    P+ G  PS S+ A                      K + 
Sbjct: 301 LPMLDKDGSGGIVALSQ---SPFAGRVPSNSATA----------------------KGEG 360

Query: 361 KQSAMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGS 420
           KQ   EEG S   +E       N+NT            S D  AIKPG+  D++FGG G+
Sbjct: 361 KQPVAEEGSSEDASERPTGDNSNLNT----------AFSFDFSAIKPGMAADVKFGGSGA 417

Query: 421 YPNLPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQN 461
            PNLPWVSTTG+GP G+TISGVTYR+NANQIKIVCACHG+HMSPEEF+RHA+ E  S ++
Sbjct: 421 RPNLPWVSTTGSGPHGRTISGVTYRYNANQIKIVCACHGSHMSPEEFVRHASEEYVSPES 417

BLAST of CmaCh12G012640 vs. ExPASy Swiss-Prot
Match: Q6AT41 (Protein NINJA homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NINJA1 PE=1 SV=1)

HSP 1 Score: 162.5 bits (410), Expect = 1.1e-38
Identity = 162/502 (32.27%), Postives = 231/502 (46.02%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSK---LTSSSGANAEE-SDRGNKLVDD--FKNFRHGD 60
           M++E+GLELSLGL+LGG   KS+++   L   +    EE S +G     +  F ++   +
Sbjct: 1   MDDENGLELSLGLSLGGTSGKSKARDAPLEPKAEPQVEESSSKGVSQTPEAPFVHYYQTN 60

Query: 61  NPRQESDTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPD 120
              QE  +    S + P   NF+      G  G S + +           A+  +E  P 
Sbjct: 61  AENQEHSSKQRHSPAAPPFGNFW------GQPGSSSVPV-----------ADGSNEQKP- 120

Query: 121 ISNKRKLLFVEMNGQKKRERESHEKGMASHIS-----------STEDGSTAEKGDVVESR 180
           +S+KRKLL  E++ QKK    + +    S  S           ST+DGST E  DV ES 
Sbjct: 121 VSSKRKLLSEEISFQKKPNTAAEQPDAFSKSSDGGVKNAPISISTDDGSTGENEDVAESE 180

Query: 181 PEGSTSRLASKHDDSYQQCISVEFLWNLPKVSKDICGISDSSRQKRFNSSS-------VG 240
            EGS S L ++ +DS +  + V    +  + S D        RQ  F+ S         G
Sbjct: 181 AEGSNSWLVAQREDSAKGSV-VNRGSDRKRSSDDAAVGFQGKRQPSFSGSESSSGKLPQG 240

Query: 241 VPNSIQVMNMHNVNTHGVPSSMGHLQLGMQHVPPTI-NGDRSGPEHVNLRNLPLTAGVSP 300
            P S+Q  N+  V  + VPS +          PP+I N     P        P   G++ 
Sbjct: 241 NPLSLQASNVVAV-PYQVPSQVS--------APPSITNASNFTPVCTVQLRPPTNNGLAV 300

Query: 301 LPISAMDKDKPWGLVSHPQMIHRPYGGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKD 360
              S       +  V  P +        T S+ +         SF      E A     D
Sbjct: 301 TMGSTSQVAFGYPAVQLPTL-------ETSSSWAFGAPPQAMSSFTAKDKVERAGISQAD 360

Query: 361 DSKQSAMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDI--PAIKPGIGTDIEFG 420
           D K++  E G SSS                ++E  K +  +L +   AI+PGI  +++FG
Sbjct: 361 DGKKT-QEAGASSSA---------------LVEDDKKSDRALPLMGSAIRPGIAPNVKFG 420

Query: 421 GCGSYPNLPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGENF 476
           G GSYP+LPWVSTTG GP G+TISGVTY+F  N++KIVCACHGTHM+PEEF+RHA+ +  
Sbjct: 421 GSGSYPDLPWVSTTGTGPNGRTISGVTYKFGRNEVKIVCACHGTHMTPEEFMRHASADAP 451

BLAST of CmaCh12G012640 vs. ExPASy Swiss-Prot
Match: B6TCE8 (Ninja-family protein 8 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 152.5 bits (384), Expect = 1.2e-35
Identity = 150/504 (29.76%), Postives = 222/504 (44.05%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSK---LTSSSGANAEE-SDRGNKLVDDFKNFRHGDNP 60
           M++++GLELSLGL+LGG+  K++++   L   +    EE S +G     D  + +     
Sbjct: 1   MDDDNGLELSLGLSLGGSSGKAKARDAPLEPKAEPQVEESSSKGGLQTPDAPSGKFYQKS 60

Query: 61  RQESDTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLP--- 120
               +  S + +S P    F N   + G     ++D   +    +   +  +D  +P   
Sbjct: 61  ADNHEQNSKQRHS-PVAPQFGNFWGQPGNSSGPVVDGSVEPVSHQPQLSSYQDGRMPINS 120

Query: 121 --------DISNKRKLLFVEMNGQKKRERESHEKGMASHIS-----------STEDGSTA 180
                    +S   KL   EMN QKK +  S +    S  S           ST+DGST 
Sbjct: 121 GNNSEEQKPVSGNHKLPSEEMNFQKKHQTASDQPDAFSKSSDGGAKNAPISISTDDGSTG 180

Query: 181 EKGDVVESRPEGSTSRLASKHDDSYQQCISVEFLWNLPKVSKDICGIS-DSSRQKRF--N 240
           E  DV ES  EGS S L ++ +DS +   SV    +  K S D         RQ  F  +
Sbjct: 181 ENEDVAESEAEGSNSWLVAQREDSAKG--SVVNKGSDRKRSADAAADGFKGKRQPSFSGS 240

Query: 241 SSSVGVPNSIQVMNMHNVNTHGVPSSMGHLQLGMQHVPPTINGDRSGPEHVNLRNLPLTA 300
            SS G      +++M   N   +P  +     G    PP +    +      ++  P T 
Sbjct: 241 ESSSGKLTPGNLISMQASNVVALPYQVP----GQVSGPPVLTNAPNFHPVCTVQLRPPTN 300

Query: 301 GVSPLPISAMDKDKPWGLVSHPQMIHRPYGGGTPSTSSVAEQVIGHFSFEGLLHGESATE 360
           G   +          +G   +P +       G+        Q +  F+ +     +    
Sbjct: 301 GGLAVQTMGSASQVAFG---YPAVQLPTLETGSSWAFGAPPQALSSFTAKD--KADQTGT 360

Query: 361 RTKDDSKQSAMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIE 420
           +  DD K+       SS+ AE               E+    G+SL   AI+PGI  +++
Sbjct: 361 KQVDDGKKPQEAGASSSAHAED--------------EKKADRGLSLMGSAIRPGIAPNVK 420

Query: 421 FGGCGSYPNLPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGE 476
           FGG GSYP+LPWVST GAGP G+TISGVTY+F  N++KIVCACHGTHMSPEEF+RHA+ +
Sbjct: 421 FGGSGSYPDLPWVSTIGAGPNGRTISGVTYKFGRNEVKIVCACHGTHMSPEEFMRHASAD 478

BLAST of CmaCh12G012640 vs. ExPASy Swiss-Prot
Match: B4FXN6 (Ninja-family protein 7 OS=Zea mays OX=4577 PE=2 SV=1)

HSP 1 Score: 152.1 bits (383), Expect = 1.5e-35
Identity = 150/504 (29.76%), Postives = 221/504 (43.85%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSK---LTSSSGANAEE-SDRGNKLVDDFKNFRHGDNP 60
           M++++GLELSLGL+LGG+  K++++   L   +    EE S +G     D  + +     
Sbjct: 1   MDDDNGLELSLGLSLGGSSGKAKARDAPLEPKAEPQVEESSSKGGLQTPDAPSGKFYQKS 60

Query: 61  RQESDTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLP--- 120
               +  S + +S P    F N   + G     ++D   +    +   +  +D  +P   
Sbjct: 61  ADNHEQNSKQRHS-PVAPQFGNFWGQPGNSSGPVVDGSVEPVSHQPQLSSYQDGRMPINS 120

Query: 121 --------DISNKRKLLFVEMNGQKKRERESHEKGMASHIS-----------STEDGSTA 180
                    +S   KL   EMN QKK +  S +    S  S           ST+DGST 
Sbjct: 121 GNNSEEQKPVSGNHKLPSEEMNFQKKHQTASDQPDAFSKSSDGGAKNAPISISTDDGSTG 180

Query: 181 EKGDVVESRPEGSTSRLASKHDDSYQQCISVEFLWNLPKVSKDICGIS-DSSRQKRF--N 240
           E  DV ES  EGS S L ++ +DS +   SV       K S D         RQ  F  +
Sbjct: 181 ENEDVAESEAEGSNSWLVAQREDSAKG--SVVNKGTDRKRSADAAADGFKGKRQPSFSGS 240

Query: 241 SSSVGVPNSIQVMNMHNVNTHGVPSSMGHLQLGMQHVPPTINGDRSGPEHVNLRNLPLTA 300
            SS G      +++M   N   +P  +     G    PP +    +      ++  P T 
Sbjct: 241 ESSSGKLTPGNLISMQASNVVALPYQVP----GQVSGPPVLTNAPNFHPVCTVQLRPPTN 300

Query: 301 GVSPLPISAMDKDKPWGLVSHPQMIHRPYGGGTPSTSSVAEQVIGHFSFEGLLHGESATE 360
           G   +          +G   +P +       G+        Q +  F+ +     +    
Sbjct: 301 GGLAVQTMGSASQVAFG---YPAVQLPTLETGSSWAFGAPPQALSSFTAKD--KADQTGT 360

Query: 361 RTKDDSKQSAMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIE 420
           +  DD K+       SS+ AE               E+    G+SL   AI+PGI  +++
Sbjct: 361 KQVDDGKKPREAGASSSAHAED--------------EKKADRGLSLMGSAIRPGIAPNVK 420

Query: 421 FGGCGSYPNLPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGE 476
           FGG GSYP+LPWVST GAGP G+TISGVTY+F  N++KIVCACHGTHMSPEEF+RHA+ +
Sbjct: 421 FGGSGSYPDLPWVSTIGAGPNGRTISGVTYKFGRNEVKIVCACHGTHMSPEEFMRHASAD 478

BLAST of CmaCh12G012640 vs. ExPASy TrEMBL
Match: A0A6J1HPH6 (ninja-family protein mc410-like OS=Cucurbita maxima OX=3661 GN=LOC111465527 PE=3 SV=1)

HSP 1 Score: 947.6 bits (2448), Expect = 2.0e-272
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60
           MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES
Sbjct: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60

Query: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120
           DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK
Sbjct: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120

Query: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180
           LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ
Sbjct: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180

Query: 181 QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH 240
           QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH
Sbjct: 181 QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH 240

Query: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300
           LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY
Sbjct: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300

Query: 301 GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN 360
           GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN
Sbjct: 301 GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN 360

Query: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420
           VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT
Sbjct: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420

Query: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 476
           YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS
Sbjct: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 475

BLAST of CmaCh12G012640 vs. ExPASy TrEMBL
Match: A0A6J1FHT3 (ninja-family protein mc410-like OS=Cucurbita moschata OX=3662 GN=LOC111444266 PE=3 SV=1)

HSP 1 Score: 871.7 bits (2251), Expect = 1.4e-249
Identity = 446/475 (93.89%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60
           MENED LELSLGLTLGGA VK RSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES
Sbjct: 1   MENEDELELSLGLTLGGASVKLRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60

Query: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120
           DTGSFESNSIPSKDNFFNDL KVGMDGDS IDLKRKGTWIESSYAEAEDENLPDISNKRK
Sbjct: 61  DTGSFESNSIPSKDNFFNDLSKVGMDGDSSIDLKRKGTWIESSYAEAEDENLPDISNKRK 120

Query: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180
           LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ
Sbjct: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180

Query: 181 QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH 240
           QCI      +LPKV         S+ QKRFNSSSVGVPNSIQVMNMHNVNTHGVPS+MGH
Sbjct: 181 QCIGDS---SLPKV---------STMQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSTMGH 240

Query: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300
           LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRP+
Sbjct: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPF 300

Query: 301 GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN 360
           GGG PSTSS AEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRA+HMKVSI N
Sbjct: 301 GGGAPSTSSAAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAKHMKVSIAN 360

Query: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420
           VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT
Sbjct: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420

Query: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 476
           YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS
Sbjct: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 463

BLAST of CmaCh12G012640 vs. ExPASy TrEMBL
Match: A0A1S3C8B6 (ninja-family protein mc410 OS=Cucumis melo OX=3656 GN=LOC103497953 PE=3 SV=1)

HSP 1 Score: 707.2 bits (1824), Expect = 4.6e-200
Identity = 375/495 (75.76%), Postives = 409/495 (82.63%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60
           ME+EDGLELSLGL+LGGA VKS+ K+TSSSGANAEESDRGNKLVDDFK+F HGDN RQES
Sbjct: 1   MESEDGLELSLGLSLGGASVKSKGKITSSSGANAEESDRGNKLVDDFKSFLHGDNERQES 60

Query: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120
           DTGSF+SNSIPSKDNFFNDL KV +DGDS IDLKRKG WIES+YAEAEDENLP+I NKRK
Sbjct: 61  DTGSFQSNSIPSKDNFFNDLSKVVVDGDSSIDLKRKGAWIESNYAEAEDENLPEIGNKRK 120

Query: 121 LLFVEMNGQKKRERES-----HEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKH 180
           LL +EMN QKK+ERES     HEKG AS+ISSTEDGSTAEKGDVVESR EGSTSRLASKH
Sbjct: 121 LLLMEMNSQKKQERESHHVDLHEKGRASYISSTEDGSTAEKGDVVESRAEGSTSRLASKH 180

Query: 181 DDSYQQCISVEFLWNLPKVSKDICGISDS-----SRQKRFNSSSVGVPNSIQVMNMHNV- 240
           DDS +QCI      +LPK SKDICG SDS     SRQKRFNSSSVGV +++Q +NMHNV 
Sbjct: 181 DDSSKQCIGET---SLPKGSKDICGFSDSSGADLSRQKRFNSSSVGVSDAVQALNMHNVH 240

Query: 241 --------NTHGVPSSMGHLQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAM 300
                   N+ G PS+ GHLQLG+QHV PT+NGDR G EH+N RNLPL  G+SPL ISAM
Sbjct: 241 FPITVKDTNSLGAPSTSGHLQLGIQHVRPTVNGDRPGAEHINPRNLPLMPGISPLQISAM 300

Query: 301 DKDKPWGLVSHPQMIHRPYGG-GTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQS 360
           DKDK WGLVSHPQMIH PYGG G  S+SS   QVIG FS +GLL+G   TE TK D+KQ 
Sbjct: 301 DKDKAWGLVSHPQMIHHPYGGVGGASSSSAPGQVIGPFSSDGLLYGGRVTELTKGDNKQP 360

Query: 361 AMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPN 420
           AMEEG SSSRAEH K S  N+N KD+LER KAAGVSLD PAIKPGI  DIEFGGCGSYPN
Sbjct: 361 AMEEG-SSSRAEHAKGSSSNMNAKDVLERSKAAGVSLDFPAIKPGIAADIEFGGCGSYPN 420

Query: 421 LPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNG 476
           LPWVSTT  GPTGKTISGVTY++NANQIKIVCACHGTHMSPEEFIRHAN E+FS QNDN 
Sbjct: 421 LPWVSTTAPGPTGKTISGVTYKYNANQIKIVCACHGTHMSPEEFIRHANDESFSVQNDNA 480

BLAST of CmaCh12G012640 vs. ExPASy TrEMBL
Match: A0A5D3CFQ4 (Ninja-family protein mc410 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold332G00430 PE=3 SV=1)

HSP 1 Score: 704.9 bits (1818), Expect = 2.3e-199
Identity = 374/495 (75.56%), Postives = 408/495 (82.42%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60
           ME+EDGLELSLGL+LGGA VKS+ K+TSSSGANAEESDRGNKLVDDFK+F HGDN RQES
Sbjct: 1   MESEDGLELSLGLSLGGASVKSKGKITSSSGANAEESDRGNKLVDDFKSFLHGDNERQES 60

Query: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120
           DTGSF+SNSIPSKDNFFNDL KV +DGDS IDLKRKG WIES+YAEAEDENLP+I NKRK
Sbjct: 61  DTGSFQSNSIPSKDNFFNDLSKVVVDGDSSIDLKRKGAWIESNYAEAEDENLPEIGNKRK 120

Query: 121 LLFVEMNGQKKRERES-----HEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKH 180
           LL +EMN QKK+ERES     HEKG AS+ISSTEDGSTAEKGDVVESR EGSTSRLASKH
Sbjct: 121 LLLMEMNSQKKQERESHHVDLHEKGRASYISSTEDGSTAEKGDVVESRAEGSTSRLASKH 180

Query: 181 DDSYQQCISVEFLWNLPKVSKDICGISDS-----SRQKRFNSSSVGVPNSIQVMNMHNV- 240
           DDS +QCI      +LPK SKDICG SDS     SRQKRFNSSSVGV +++Q +NMHNV 
Sbjct: 181 DDSSKQCIGET---SLPKGSKDICGFSDSSGADLSRQKRFNSSSVGVSDAVQALNMHNVH 240

Query: 241 --------NTHGVPSSMGHLQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAM 300
                   N+ G PS+ GHLQLG+QHV PT+NGDR G EH+N RNLPL  G+SPL ISAM
Sbjct: 241 FPITVKDTNSLGAPSTSGHLQLGIQHVRPTVNGDRPGAEHINPRNLPLMPGISPLQISAM 300

Query: 301 DKDKPWGLVSHPQMIHRPYGG-GTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQS 360
           DKDK WGLVSHPQMIH PYGG G  S+SS   QVIG FS +GLL+G   TE TK D+KQ 
Sbjct: 301 DKDKAWGLVSHPQMIHHPYGGVGGASSSSAPGQVIGPFSSDGLLYGGRVTELTKGDNKQP 360

Query: 361 AMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPN 420
           AMEEG SSSRAEH K S  N+N KD+LER KAAGVSLD PAIKPGI  DIEFGGCG YPN
Sbjct: 361 AMEEG-SSSRAEHAKGSSSNMNAKDVLERSKAAGVSLDFPAIKPGIAADIEFGGCGLYPN 420

Query: 421 LPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNG 476
           LPWVSTT  GPTGKTISGVTY++NANQIKIVCACHGTHMSPEEFIRHAN E+FS QNDN 
Sbjct: 421 LPWVSTTAPGPTGKTISGVTYKYNANQIKIVCACHGTHMSPEEFIRHANDESFSVQNDNA 480

BLAST of CmaCh12G012640 vs. ExPASy TrEMBL
Match: A0A5A7V2Q1 (Ninja-family protein mc410 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold41G001020 PE=3 SV=1)

HSP 1 Score: 704.1 bits (1816), Expect = 3.9e-199
Identity = 373/495 (75.35%), Postives = 409/495 (82.63%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60
           ME+EDGLELSLGL+LGGA VKS+ K+TSSSGANAEESDRG+KLVDDFK+F HGDN RQES
Sbjct: 1   MESEDGLELSLGLSLGGASVKSKGKITSSSGANAEESDRGSKLVDDFKSFLHGDNERQES 60

Query: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120
           DTGSF+SNSIPSKDNFFNDL KV +DGDS IDLKRKG WIES++AEAEDENLP+I NKRK
Sbjct: 61  DTGSFQSNSIPSKDNFFNDLSKVVVDGDSSIDLKRKGAWIESNHAEAEDENLPEIGNKRK 120

Query: 121 LLFVEMNGQKKRERES-----HEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKH 180
           LL +EMN QKK+ERES     HEKG AS+ISSTEDGSTAEKGDVVESR EGSTSRLASKH
Sbjct: 121 LLLMEMNSQKKQERESHHVDLHEKGRASYISSTEDGSTAEKGDVVESRAEGSTSRLASKH 180

Query: 181 DDSYQQCISVEFLWNLPKVSKDICGISDS-----SRQKRFNSSSVGVPNSIQVMNMHNV- 240
           DDS +QCI      +LPK SKDICG SDS     SRQKRFNSSSVGV +++Q +NMHNV 
Sbjct: 181 DDSSKQCIGET---SLPKGSKDICGFSDSSGADLSRQKRFNSSSVGVSDAVQALNMHNVP 240

Query: 241 --------NTHGVPSSMGHLQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAM 300
                   N+ G PS+ GHLQLG+QHV PT+NGDR G EH+N RNLPL  G+SPL ISAM
Sbjct: 241 FPITVKDTNSLGAPSTSGHLQLGIQHVRPTVNGDRPGAEHINPRNLPLMPGISPLQISAM 300

Query: 301 DKDKPWGLVSHPQMIHRPYGGGT-PSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQS 360
           DKDK WGLVSHPQMIH PYGGG   S+SS   QVIG FS +GLL+G   TE TK D+KQ 
Sbjct: 301 DKDKAWGLVSHPQMIHHPYGGGVGASSSSAPGQVIGPFSSDGLLYGGRVTELTKGDNKQP 360

Query: 361 AMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPN 420
           AMEEG SSSR EH K S  N+N KD+LER KAAGVSLD PAIKPGI  DIEFGGCGSYPN
Sbjct: 361 AMEEG-SSSRVEHAKGSSSNMNAKDVLERSKAAGVSLDFPAIKPGIAADIEFGGCGSYPN 420

Query: 421 LPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNG 476
           LPWVSTT  GPTGKTISGVTY++NANQIKIVCACHGTHMSPEEFIRHAN E+FSAQNDN 
Sbjct: 421 LPWVSTTAPGPTGKTISGVTYKYNANQIKIVCACHGTHMSPEEFIRHANDESFSAQNDNA 480

BLAST of CmaCh12G012640 vs. NCBI nr
Match: XP_022965709.1 (ninja-family protein mc410-like [Cucurbita maxima] >XP_022965710.1 ninja-family protein mc410-like [Cucurbita maxima])

HSP 1 Score: 947.6 bits (2448), Expect = 4.2e-272
Identity = 475/475 (100.00%), Postives = 475/475 (100.00%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60
           MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES
Sbjct: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60

Query: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120
           DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK
Sbjct: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120

Query: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180
           LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ
Sbjct: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180

Query: 181 QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH 240
           QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH
Sbjct: 181 QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH 240

Query: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300
           LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY
Sbjct: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300

Query: 301 GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN 360
           GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN
Sbjct: 301 GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN 360

Query: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420
           VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT
Sbjct: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420

Query: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 476
           YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS
Sbjct: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 475

BLAST of CmaCh12G012640 vs. NCBI nr
Match: KAG7021297.1 (Ninja-family protein, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 877.1 bits (2265), Expect = 6.9e-251
Identity = 449/475 (94.53%), Postives = 452/475 (95.16%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60
           MENED LELSLGLTLGGA VKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES
Sbjct: 1   MENEDELELSLGLTLGGASVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60

Query: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120
           DTGSFESNSIPSKDNFFNDL KVGMDGDS IDLKRKGTWIESSYAEAEDENLPDISNKRK
Sbjct: 61  DTGSFESNSIPSKDNFFNDLSKVGMDGDSSIDLKRKGTWIESSYAEAEDENLPDISNKRK 120

Query: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180
           LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ
Sbjct: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180

Query: 181 QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH 240
           QCI      +LPKV         S+ QKRFNSSSVGVPNSIQVMNMHNVNTHGVPS+MGH
Sbjct: 181 QCIGDS---SLPKV---------STMQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSTMGH 240

Query: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300
           LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY
Sbjct: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300

Query: 301 GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN 360
           GGG PSTSS AEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSI N
Sbjct: 301 GGGAPSTSSAAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIAN 360

Query: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420
           VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT
Sbjct: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420

Query: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 476
           YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS
Sbjct: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 463

BLAST of CmaCh12G012640 vs. NCBI nr
Match: XP_022938067.1 (ninja-family protein mc410-like [Cucurbita moschata] >XP_022938068.1 ninja-family protein mc410-like [Cucurbita moschata])

HSP 1 Score: 871.7 bits (2251), Expect = 2.9e-249
Identity = 446/475 (93.89%), Postives = 451/475 (94.95%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60
           MENED LELSLGLTLGGA VK RSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES
Sbjct: 1   MENEDELELSLGLTLGGASVKLRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60

Query: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120
           DTGSFESNSIPSKDNFFNDL KVGMDGDS IDLKRKGTWIESSYAEAEDENLPDISNKRK
Sbjct: 61  DTGSFESNSIPSKDNFFNDLSKVGMDGDSSIDLKRKGTWIESSYAEAEDENLPDISNKRK 120

Query: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180
           LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ
Sbjct: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180

Query: 181 QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH 240
           QCI      +LPKV         S+ QKRFNSSSVGVPNSIQVMNMHNVNTHGVPS+MGH
Sbjct: 181 QCIGDS---SLPKV---------STMQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSTMGH 240

Query: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300
           LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRP+
Sbjct: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPF 300

Query: 301 GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN 360
           GGG PSTSS AEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRA+HMKVSI N
Sbjct: 301 GGGAPSTSSAAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAKHMKVSIAN 360

Query: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420
           VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT
Sbjct: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420

Query: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 476
           YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS
Sbjct: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 463

BLAST of CmaCh12G012640 vs. NCBI nr
Match: XP_023536660.1 (ninja-family protein mc410-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 869.8 bits (2246), Expect = 1.1e-248
Identity = 446/475 (93.89%), Postives = 449/475 (94.53%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60
           MENED LELSLGLTLG A VKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES
Sbjct: 1   MENEDELELSLGLTLGAASVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60

Query: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120
            TGSFESNSIPSKDNFFNDL KVGMDGDS IDLKRKGTWIESSYAEAEDENLPDISNKRK
Sbjct: 61  GTGSFESNSIPSKDNFFNDLSKVGMDGDSSIDLKRKGTWIESSYAEAEDENLPDISNKRK 120

Query: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180
           LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ
Sbjct: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180

Query: 181 QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH 240
           QCI      +LPKV         S+ QKRFNSSSVGVPNSIQVMNMHNVNTHGVPS+MGH
Sbjct: 181 QCIGDS---SLPKV---------STMQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSTMGH 240

Query: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300
           LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY
Sbjct: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300

Query: 301 GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN 360
           GGG PSTSS AEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSI N
Sbjct: 301 GGGAPSTSSAAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIAN 360

Query: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420
           VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT
Sbjct: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420

Query: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 476
           YRFNANQIKIVCACHG HMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS
Sbjct: 421 YRFNANQIKIVCACHGIHMSPEEFIRHANGENFSAQNDNGPATIPNKNPTASARS 463

BLAST of CmaCh12G012640 vs. NCBI nr
Match: KAG6586442.1 (Ninja-family protein 410, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 840.9 bits (2171), Expect = 5.5e-240
Identity = 430/459 (93.68%), Postives = 434/459 (94.55%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60
           MENED LELSLGLTLGGA VKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES
Sbjct: 1   MENEDELELSLGLTLGGASVKSRSKLTSSSGANAEESDRGNKLVDDFKNFRHGDNPRQES 60

Query: 61  DTGSFESNSIPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLPDISNKRK 120
           DTGSFESNSIPSKDNFFNDL KVGMDGDS IDLKRKGTWIESSYAEAEDENLPDISNKRK
Sbjct: 61  DTGSFESNSIPSKDNFFNDLSKVGMDGDSSIDLKRKGTWIESSYAEAEDENLPDISNKRK 120

Query: 121 LLFVEMNGQKKRERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180
           LLFVEMNGQKK ERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ
Sbjct: 121 LLFVEMNGQKKHERESHEKGMASHISSTEDGSTAEKGDVVESRPEGSTSRLASKHDDSYQ 180

Query: 181 QCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSSMGH 240
           QCI      +LPKV         S+ QKRFNSSSVGVPNSIQVMNMHNVNTHGVPS+MGH
Sbjct: 181 QCIGDS---SLPKV---------STMQKRFNSSSVGVPNSIQVMNMHNVNTHGVPSTMGH 240

Query: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300
           LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY
Sbjct: 241 LQLGMQHVPPTINGDRSGPEHVNLRNLPLTAGVSPLPISAMDKDKPWGLVSHPQMIHRPY 300

Query: 301 GGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSSRAEHMKVSIPN 360
           G G PSTSS AEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLS+SRAEHMKVSI N
Sbjct: 301 GRGAPSTSSAAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSTSRAEHMKVSIAN 360

Query: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420
           VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT
Sbjct: 361 VNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTGAGPTGKTISGVT 420

Query: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDN 460
           YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDN
Sbjct: 421 YRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQNDN 447

BLAST of CmaCh12G012640 vs. TAIR 10
Match: AT4G28910.2 (novel interactor of JAZ )

HSP 1 Score: 233.4 bits (594), Expect = 3.7e-61
Identity = 177/483 (36.65%), Postives = 249/483 (51.55%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGAN-----AEESDRGNKLVDDFKNFRHGDN 60
           M++++GLELSLGL+ GG+  K++    +++G++     AE  DR  K++DDFKNF H  +
Sbjct: 1   MDDDNGLELSLGLSCGGSTGKAKGNNNNNAGSSSENYRAEGGDRSAKVIDDFKNFLHPTS 60

Query: 61  PR-QESDTGSFESNS-IPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLP 120
            R  E  +GS  S+S      NFFNDL K      +  +   K  W+       EDE+  
Sbjct: 61  QRPAEPSSGSQRSDSGQQPPQNFFNDLSKA---PTTEAEASTKPLWV-------EDESRK 120

Query: 121 DISNKRKLLFVEMNGQKKRERES-----HE-KGMASHIS-STEDGSTAEKGDVVESRPEG 180
           +  NKRK  F  MN  KK+E++S     HE K  ASH+S +T++GSTAE  DV ES   G
Sbjct: 121 EAGNKRKFGFPGMNDDKKKEKDSSHVDMHEKKTKASHVSTATDEGSTAENEDVAESEVGG 180

Query: 181 STSRLASKHDDSYQQCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNM 240
            +S   +K                + +   D   + + + Q+R N    G     +   M
Sbjct: 181 GSSSNHAK---------------EVVRPPTDTNIVDNLTGQRRSNHGGSGT----EEFTM 240

Query: 241 HNVNTHGVPSSMGHLQLGMQHVPPTINGDRSGPE---------HVNLRNLPLTAGVSPLP 300
            N+ ++ VP ++ H Q  +  +P ++    SG           + N  NLP+  G SP+ 
Sbjct: 241 RNM-SYTVPFTV-HPQNVVTSMPYSLPTKESGQHAAATSLLQPNANAGNLPIMFGYSPVQ 300

Query: 301 ISAMDKDKPWGLVSHPQMIHRPYGGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDS 360
           +  +DKD   G+V+  Q    P+ G  PS S+ A                      K + 
Sbjct: 301 LPMLDKDGSGGIVALSQ---SPFAGRVPSNSATA----------------------KGEG 360

Query: 361 KQSAMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGS 420
           KQ   EEG S   +E       N+NT            S D  AIKPG+  D++FGG G+
Sbjct: 361 KQPVAEEGSSEDASERPTGDNSNLNT----------AFSFDFSAIKPGMAADVKFGGSGA 417

Query: 421 YPNLPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQN 461
            PNLPWVSTTG+GP G+TISGVTYR+NANQIKIVCACHG+HMSPEEF+RHA+ E  S ++
Sbjct: 421 RPNLPWVSTTGSGPHGRTISGVTYRYNANQIKIVCACHGSHMSPEEFVRHASEEYVSPES 417

BLAST of CmaCh12G012640 vs. TAIR 10
Match: AT4G28910.1 (novel interactor of JAZ )

HSP 1 Score: 233.4 bits (594), Expect = 3.7e-61
Identity = 177/483 (36.65%), Postives = 249/483 (51.55%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGAN-----AEESDRGNKLVDDFKNFRHGDN 60
           M++++GLELSLGL+ GG+  K++    +++G++     AE  DR  K++DDFKNF H  +
Sbjct: 1   MDDDNGLELSLGLSCGGSTGKAKGNNNNNAGSSSENYRAEGGDRSAKVIDDFKNFLHPTS 60

Query: 61  PR-QESDTGSFESNS-IPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLP 120
            R  E  +GS  S+S      NFFNDL K      +  +   K  W+       EDE+  
Sbjct: 61  QRPAEPSSGSQRSDSGQQPPQNFFNDLSKA---PTTEAEASTKPLWV-------EDESRK 120

Query: 121 DISNKRKLLFVEMNGQKKRERES-----HE-KGMASHIS-STEDGSTAEKGDVVESRPEG 180
           +  NKRK  F  MN  KK+E++S     HE K  ASH+S +T++GSTAE  DV ES   G
Sbjct: 121 EAGNKRKFGFPGMNDDKKKEKDSSHVDMHEKKTKASHVSTATDEGSTAENEDVAESEVGG 180

Query: 181 STSRLASKHDDSYQQCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNM 240
            +S   +K                + +   D   + + + Q+R N    G     +   M
Sbjct: 181 GSSSNHAK---------------EVVRPPTDTNIVDNLTGQRRSNHGGSGT----EEFTM 240

Query: 241 HNVNTHGVPSSMGHLQLGMQHVPPTINGDRSGPE---------HVNLRNLPLTAGVSPLP 300
            N+ ++ VP ++ H Q  +  +P ++    SG           + N  NLP+  G SP+ 
Sbjct: 241 RNM-SYTVPFTV-HPQNVVTSMPYSLPTKESGQHAAATSLLQPNANAGNLPIMFGYSPVQ 300

Query: 301 ISAMDKDKPWGLVSHPQMIHRPYGGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDS 360
           +  +DKD   G+V+  Q    P+ G  PS S+ A                      K + 
Sbjct: 301 LPMLDKDGSGGIVALSQ---SPFAGRVPSNSATA----------------------KGEG 360

Query: 361 KQSAMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGS 420
           KQ   EEG S   +E       N+NT            S D  AIKPG+  D++FGG G+
Sbjct: 361 KQPVAEEGSSEDASERPTGDNSNLNT----------AFSFDFSAIKPGMAADVKFGGSGA 417

Query: 421 YPNLPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQN 461
            PNLPWVSTTG+GP G+TISGVTYR+NANQIKIVCACHG+HMSPEEF+RHA+ E  S ++
Sbjct: 421 RPNLPWVSTTGSGPHGRTISGVTYRYNANQIKIVCACHGSHMSPEEFVRHASEEYVSPES 417

BLAST of CmaCh12G012640 vs. TAIR 10
Match: AT4G28910.3 (novel interactor of JAZ )

HSP 1 Score: 233.4 bits (594), Expect = 3.7e-61
Identity = 177/483 (36.65%), Postives = 249/483 (51.55%), Query Frame = 0

Query: 1   MENEDGLELSLGLTLGGACVKSRSKLTSSSGAN-----AEESDRGNKLVDDFKNFRHGDN 60
           M++++GLELSLGL+ GG+  K++    +++G++     AE  DR  K++DDFKNF H  +
Sbjct: 1   MDDDNGLELSLGLSCGGSTGKAKGNNNNNAGSSSENYRAEGGDRSAKVIDDFKNFLHPTS 60

Query: 61  PR-QESDTGSFESNS-IPSKDNFFNDLLKVGMDGDSLIDLKRKGTWIESSYAEAEDENLP 120
            R  E  +GS  S+S      NFFNDL K      +  +   K  W+       EDE+  
Sbjct: 61  QRPAEPSSGSQRSDSGQQPPQNFFNDLSKA---PTTEAEASTKPLWV-------EDESRK 120

Query: 121 DISNKRKLLFVEMNGQKKRERES-----HE-KGMASHIS-STEDGSTAEKGDVVESRPEG 180
           +  NKRK  F  MN  KK+E++S     HE K  ASH+S +T++GSTAE  DV ES   G
Sbjct: 121 EAGNKRKFGFPGMNDDKKKEKDSSHVDMHEKKTKASHVSTATDEGSTAENEDVAESEVGG 180

Query: 181 STSRLASKHDDSYQQCISVEFLWNLPKVSKDICGISDSSRQKRFNSSSVGVPNSIQVMNM 240
            +S   +K                + +   D   + + + Q+R N    G     +   M
Sbjct: 181 GSSSNHAK---------------EVVRPPTDTNIVDNLTGQRRSNHGGSGT----EEFTM 240

Query: 241 HNVNTHGVPSSMGHLQLGMQHVPPTINGDRSGPE---------HVNLRNLPLTAGVSPLP 300
            N+ ++ VP ++ H Q  +  +P ++    SG           + N  NLP+  G SP+ 
Sbjct: 241 RNM-SYTVPFTV-HPQNVVTSMPYSLPTKESGQHAAATSLLQPNANAGNLPIMFGYSPVQ 300

Query: 301 ISAMDKDKPWGLVSHPQMIHRPYGGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDS 360
           +  +DKD   G+V+  Q    P+ G  PS S+ A                      K + 
Sbjct: 301 LPMLDKDGSGGIVALSQ---SPFAGRVPSNSATA----------------------KGEG 360

Query: 361 KQSAMEEGLSSSRAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGS 420
           KQ   EEG S   +E       N+NT            S D  AIKPG+  D++FGG G+
Sbjct: 361 KQPVAEEGSSEDASERPTGDNSNLNT----------AFSFDFSAIKPGMAADVKFGGSGA 417

Query: 421 YPNLPWVSTTGAGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGENFSAQN 461
            PNLPWVSTTG+GP G+TISGVTYR+NANQIKIVCACHG+HMSPEEF+RHA+ E  S ++
Sbjct: 421 RPNLPWVSTTGSGPHGRTISGVTYRYNANQIKIVCACHGSHMSPEEFVRHASEEYVSPES 417

BLAST of CmaCh12G012640 vs. TAIR 10
Match: AT3G07250.1 (nuclear transport factor 2 (NTF2) family protein / RNA recognition motif (RRM)-containing protein )

HSP 1 Score: 70.5 bits (171), Expect = 4.2e-12
Identity = 51/166 (30.72%), Postives = 71/166 (42.77%), Query Frame = 0

Query: 290 VSHPQMIHRPYGGGTPSTSSVAEQVIGHFSFEGLLHGESATERTKDDSKQSAMEEGLSSS 349
           VS P    R  GG     +   +          +L G   T    D SK +  +E  +SS
Sbjct: 134 VSDPSSSSRQEGGSGDIGAQSGQTKQVRSPVNNILTGTEQTVHNTDGSKDAETQEWSNSS 193

Query: 350 -RAEHMKVSIPNVNTKDILERPKAAGVSLDIPAIKPGIGTDIEFGGCGSYPNLPWVSTTG 409
              E  K   P+ N+                     G G+ + F        +P V++TG
Sbjct: 194 VTKESGKPPKPHTNSN--------------------GNGSLLPFA------QMPCVTSTG 253

Query: 410 AGPTGKTISGVTYRFNANQIKIVCACHGTHMSPEEFIRHANGENFS 455
            GP GKT++G  YR++ ++I I+C CHGT  SP EFI HA G N S
Sbjct: 254 NGPEGKTVNGFLYRYSKSEISIICVCHGTSFSPAEFIVHAGGTNVS 273

BLAST of CmaCh12G012640 vs. TAIR 10
Match: AT3G29575.1 (ABI five binding protein 3 )

HSP 1 Score: 67.0 bits (162), Expect = 4.6e-11
Identity = 27/52 (51.92%), Postives = 39/52 (75.00%), Query Frame = 0

Query: 400 NLPWVSTTGAGPTGKTISGVTYRF-NANQIKIVCACHGTHMSPEEFIRHANG 451
           ++P+VSTTG GP GK I+G  YR+    +++IVC CHG+ +SP EF++HA G
Sbjct: 162 DMPFVSTTGDGPNGKKINGFLYRYRKGEEVRIVCVCHGSFLSPAEFVKHAGG 213

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q53HY28.9e-7637.62Ninja-family protein mc410 OS=Nicotiana tabacum OX=4097 GN=MC410 PE=2 SV=1[more]
Q9SV555.3e-6036.65AFP homolog 2 OS=Arabidopsis thaliana OX=3702 GN=AFPH2 PE=1 SV=1[more]
Q6AT411.1e-3832.27Protein NINJA homolog 1 OS=Oryza sativa subsp. japonica OX=39947 GN=NINJA1 PE=1 ... [more]
B6TCE81.2e-3529.76Ninja-family protein 8 OS=Zea mays OX=4577 PE=2 SV=1[more]
B4FXN61.5e-3529.76Ninja-family protein 7 OS=Zea mays OX=4577 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1HPH62.0e-272100.00ninja-family protein mc410-like OS=Cucurbita maxima OX=3661 GN=LOC111465527 PE=3... [more]
A0A6J1FHT31.4e-24993.89ninja-family protein mc410-like OS=Cucurbita moschata OX=3662 GN=LOC111444266 PE... [more]
A0A1S3C8B64.6e-20075.76ninja-family protein mc410 OS=Cucumis melo OX=3656 GN=LOC103497953 PE=3 SV=1[more]
A0A5D3CFQ42.3e-19975.56Ninja-family protein mc410 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaff... [more]
A0A5A7V2Q13.9e-19975.35Ninja-family protein mc410 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaff... [more]
Match NameE-valueIdentityDescription
XP_022965709.14.2e-272100.00ninja-family protein mc410-like [Cucurbita maxima] >XP_022965710.1 ninja-family ... [more]
KAG7021297.16.9e-25194.53Ninja-family protein, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
XP_022938067.12.9e-24993.89ninja-family protein mc410-like [Cucurbita moschata] >XP_022938068.1 ninja-famil... [more]
XP_023536660.11.1e-24893.89ninja-family protein mc410-like [Cucurbita pepo subsp. pepo][more]
KAG6586442.15.5e-24093.68Ninja-family protein 410, partial [Cucurbita argyrosperma subsp. sororia][more]
Match NameE-valueIdentityDescription
AT4G28910.23.7e-6136.65novel interactor of JAZ [more]
AT4G28910.13.7e-6136.65novel interactor of JAZ [more]
AT4G28910.33.7e-6136.65novel interactor of JAZ [more]
AT3G07250.14.2e-1230.72nuclear transport factor 2 (NTF2) family protein / RNA recognition motif (RRM)-c... [more]
AT3G29575.14.6e-1151.92ABI five binding protein 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR032308Jas TPL-binding domainPFAMPF16135TDBDcoord: 429..452
e-value: 3.4E-5
score: 23.8
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 451..475
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 38..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..144
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 23..70
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 327..341
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 326..354
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 128..175
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 154..172
NoneNo IPR availablePANTHERPTHR31413:SF12AFP HOMOLOG 2coord: 1..473
IPR031307Ninja familyPANTHERPTHR31413AFP HOMOLOG 2coord: 1..473

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G012640.1CmaCh12G012640.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0009867 jasmonic acid mediated signaling pathway
biological_process GO:0045892 negative regulation of transcription, DNA-templated
biological_process GO:0007165 signal transduction
cellular_component GO:0005634 nucleus