CmaCh12G012350 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G012350
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	tRNA (guanine(37)-N1)-methyltransferase
Location	Cma_Chr12: 9663436 .. 9669192 (-)
RNA-Seq Expression	CmaCh12G012350
Synteny	CmaCh12G012350

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

TGTAACCAAGTAGAGAACTGATTGCTTAAGCTTTAAACCCTAGTCCCGTCGCCGCCGTCTTAACTCAAGTCTCCGGCGACAGTTTGCGTTCAGAATCTTCCCCACTACCAGTGAAAATCTCCATTCTGATTTTGTTTGTTCCCAGCGAAAATCTCCGAGCTTATTCATCTATTTTCAGTTTTTTTTATTGCCGGAGGACGATAATTGTTCTATTCTGATCTGAAATTCTTATTGAGTTCGGTGTTTTTTTTTTTTGTTGTACTATCGAGTATTGAGGAAAATCGAACTTGTAAATCGACCAATGTTGGACGAAAGCAAATTCGATGTGCATTTGAAACTCTGGGCGCTAAGAATTCCCAGGGAACTCTGTAAATCCGCCATTAAAATACTGAACGGGTACGTCTTGTTTTTTGTTGCTTAGTTTCTTAATTGTACATTTCTTCGTTTCTTAGTTGAACTGTTCATCGTTCTCATCCGAGTTCGACAATCTCTATTTTCTTGAAAATATTGTTTCGATATTGCCTTTCATTTTGAAGGTAGATTGTTCTTCGGTCTCATTTCGTTTATTTTGTAAACTTTCTAGGTACTTAATTGATAGGCCCCGGATTAAACCTGTGACGGAAGATCCAACATGTGATAAGAATCGGTATGTTATATTATCGGAAAAAGTTCAATCTACAGGTAAAATTCTCTGTCATTGCACTTACTGACTTGGTTTTCTCTGTTCATTATGCTCTTTAATAAGACTTTCTGTGAGCCAAAGCTCTTTAAAATGGCATCCTACGTTTCTTCTTCCCTTTTTAGTCCTTTTTTTTATGGAAATTTCATCGACCATGTAATAGATGACACCTTATTCTTTGTTGTGATTCTTTGAATGGCTCAGAGTTGTCCGAAATTCCAGAAATGAAGCTTGAAGAGCTGAAAGGTTTATGCGAGATTGAACTAGTTCCTTATTCACTAACTCTTGGATATTCTTATTGGGGAACAGGTAGTTTCAATAATCTCATATTTTATTTTATGCATCGTGCAAGTTTGACTTGACGCATTGTGTGTAACGACCCTATTTTTCTTTATAAAATAGAGATGTCACTACATACATGACATGTCTAATTTGTGTGTAAAAGTTTGTGAAAATATTTCATAACGATGCCATGGACGTAAACGTTTATAATAAATTCATTAAAACAATATTGTTATGAACAACATAGCGGAAAATAGATAATATGAATTTGAATATAAAAACGAGACAATGCCCTGATCTAACCTGAGGGCTGCTAAAACCTACTACTCTAGGTTTGGTCCCATGACCTTTACTATAACGTTGCTTTCCGTATAGGGATGTCTTACCTTAACTTGAAAAATAAGAGTAGCACATGACTTGAGTATTTTAAGAAATATTCAGTAAGTAGTCTCACTATTGGGGTCGGAAAATGCAAACACAAGCAATCATGTTGGGACCTTTCATTGCGTAAAAACATCATGTCATGACCTTGGTCTCGACTTCCTGTCCGGGAAGTGTTGTATGTTGCTGCAGATGGGCTGATGAACCCATGTTGTCTTTGACGGTTTGATATTATGATGATTGCATATACCGTGTCTACTTTTCAGTGATAGTTTAAACTACTATATATGTGCATTTACTTGACATTTGATGTGTCCATTTTGTTTATGGCCGGCTAACTTACCGTGTGGCTCTTATGTAGATCATGTACTGAAGCAGATTCTGCCTCATGGAGTGGAAGTGCCATCATCTTTTGAAACAATAGTTAAGCACTCAAGCCATTGCCATATTTTGCCTTCCTTTTCCTTTCTACCTTACATTTGCTTGTGCTCTTTATAGCTTTTACGTGCATTCTTTTCTTCTCCTATATGGCTTTTAGGTCAGCATTATTCTGTGTTCTTTATTACAACTATTGACAGTGAGGTGATGTTGTTCTGTGATTTTACAGGTCACGTAGCTCACCTTAATATACACGACGAATTGCTCCCTTATAAGGAAGTCATTGCGAAGGTTATTTATGATGTACGTCATCAAGATCCATTTACTCATTTTTTCCAATGAATTTGAAACGTAAATTGTGAATAAATGATCATTATACTATATTTGTTACTTCATTTTCAATGCTTTTAAGTGTTTTTGTGTAATAGCTCAAGCCCATCGCTAACCGATGTTGTCCTCTTTGGGCTTCCCTTTCGGATTTCCCCTCAAGATTTTTTGAACGCGTCTCCTCGGGAGAGGTTTCTACACCTTTATAAAGAATATTTCGTTCTCCTTCCCAACTGATGTGGGATCTCACAATCCACCCCCTGGCCCAACGTTCTCATCGACACTCGTTCCCTTCTCCAATCGATGTGGGACTCCCCCAATCTACCTCCTTCGTGGCCTAACGTCCTTGCTGGCACACCACCTCATTCCGCCCCCCTTCGGGATTCAGCCTCCTTACTGGCACATTGGCCGATGTTTGGTTCTGATACCATTTATAATAGCCTAAGCCCACCACTAGTAGATATTGTTCTCTCTGGGCTTTCCCTTTTGGGTTTCCCCCCAAGGTTTTTAAAACATGTCTCCTAGAGAGAAGTTTCCACACCTTTATAAAGAATGTTTTGTTCTCCTAGCCAACCGATGTGGGATCTCATACTTTGTGTCTGCGTCCGTGTTTGAGGGCGAGGGCGTGCTAATGATTTAGCAAGAATCAGATTTCTTTATTGCTTTTAGTGCCATTGTTGGTCCCTTTCCCCCTCATTTTCCCTATAATAACTTTTCTATAACTAATTCTTTTGATTATTACGTGGGGTATGGAGGTTGAAACCTACCTCTCTAGTAGAGAGTTGAAAATTTTTAATCGTTGAACTATGCTCGGTTATTCGTTTGTAATTATTTCTTTTCAAAATCTAATTGCAGAAAAATTATCCGAGAATTAAAACAGTGGTAAATAAGGTGGGGTCGATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAGTTCTGAAGGGAGAGAATGATATGGAAACAGAAGTGAAACAATATGGAGCAACTTTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGGTTAGAGCATGAACACATAAGACTGGTTTCACTGTTCCAACCAGGAGAGATAATTTGTGACATGTTCGCCGGTATTGGTCCTTTTGCTATTCCAGCTGCCCAGAAAGGCTGTATAGTATATGCCAATGACTTGAACCCGGATAGTGTTCGATATCTGAAGATTAATGCAGAAATCAATAAAGTCGGTGATCGTGTTCATGTTCATAATTTGGACGCTAGGAAGTTCATTTCTCAATTGATGACCGTGCCAGACAATGAATGTAATTCTGAATCTGTTGCCTCGGTGTTAAAAGCTTGTGAGAAAGGTATCGGAGGGACCGCTTAGTATCATCATTGAAGGAAATTTTCATAATAGAGATTGTGTATCGTCGATCTTAATTTTCATTCTTGCTTCAGTTCAGGTGGAGGAAGTCCATGGTAGTGTTAAAACTAGCGATGAAAGTGTAGAAGGCTCAAGGAGTGCTGATGTATCTGTTGCTGCTCTTAAAAGATCCTCAGATTGCTGTGAGAAAGGTAAAAAATTCATCACTATATCTTGTGATTGAACAATGAAAGATCATCTCTTCTTATAATTTGCGGTGCAGATAATGGAATAGCTGATGATGCTTCCATCTGTGTAGCTGGTAAGAGAAAAGGAAACAGTAACAAGAGGTTGAAGGGGTCCGAAATATCTCATGCTAAGACCTGGGAGCATGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCCTTACAGTTTCTCGGTATGAACGTTTTTTTTCGGGTTTATTTCATTTACCTTGTCGTGGTTGTTATGCATATTCCTATACATTTTTTAATGTTGGAGGAAGAATTGAAACTTGGGAAATTATCAATGTTAGCTAGCCCACCTCTGCCGGTATGAGATTAGAGGGAAAATTATGGAAAGGGAAACAATAAGTCTTTGATTGTAACGTCCCAAACCCACCGCTAGTAGATATTATCCTGTTTAGGTTTTCCCTTTCAGGCTTCCCCTCAAGGTTTCTAAAATGTGTCTGGGATCTAAGAAGTTTCCACACCCTTATAGAGAATGCTTCGTTCCCCTCTCCAACCGATGTAGGATCTCACAATTCACCCTCCTTTAGGGCCCTGCATCCTTACTGACACTCGTTTCCTTCTCCAACCGATTTGGGATCTCACACGGGTCGTTCAGATGTGAAGGGAAATCAGAGATTTTTTTTTCTTTTCTTTCGAGCACAATGGTGGAGATGTTTGAGATATGTCATCTTTACCCACCTTTCAAATGAAGTTCTCTATTCAAGATTCTGTAAACACTCTCCCTTCGTGGTTGATACCGATTGTAGAGTTTCTTCAATCTCTCTTAGCTCTTGACTGAGGGAGAATTTCTCATCTTCTACGGAGATATATAGAAAGAGAGCATCAATTGAAGCTTACACGAATGGAGACCTTATATTTGTGAGATCCCATATCGGTTGGAGAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCTCTAAGAGACGCATTTTAAAACCTTGAGGGGAAATCTCGGAAGGGAAAGTTCAAAAAGAACAATATCTACTAGCGGTGGGCTTGAGCTGTTACAGATAGTATTAGAGCCAGATATCGAGCGATGTGCCAGCGAGGAAGCTGAACCCCGAAGGGGGTGGACAAGAGACCGTGTGTCAGCAAGAACGTTGGGTCCCGAAGGAGGATAGATTGTGAGATCCCACGTCAGTTGGAGAAGAGAATGAAATATTTTTTATAAGAGTGTTGAAATCTCTCCCTAGCAGCGTTTTATAACCTTGAGGGGAAACATAGAAAGAAAAGCTCAAAGCGAGAGATTGGCGGTTACAATATTAGTATGCTACCATTTTCTTACCAGAGGCATATTTTGGAATTTTCTTACCAGATGCATTTAGGGGCTCGATTCCGCTGAAGTACTGGAAGGGCTCCCTCCCTTGGATTCACTGTTATTGCTTCATGCGAGCAAATGAGAATCAAGAGTTCATCATTTCGGTATGTTCAGACTCCTATTTTGATATTCGTCTAGCAACATCATCTTTCTTCTCATGCTATCTTCATAACTGTTCATTCCAGGTGGCCGAGTCTGCCTTGAATGCTAAAATCCAAGACCCTATTTTTCATAGAGTCAGAGATGTTGCACCAAACAAGGTATTGCTCTCTATTTGTTCTTTAAACTTATTTTGTTTATGAAAAACCAAGGAACAAATTAGGGCATACAAGAACTAAAACAAAAGAGGCCCCAATCAAACTTCCATTGTTCTTAACTTCTTATATTTTTTGTTTTGATAGGCAATGTATTGTTTAAGCTTTAGGCTTCCACAACAATGCTTCAACGAAGAGTTAGCTAGCTGAATCGTATCCGTTGCAGTCGGTCGGGATGCTTACTAAGCCAAGTTTTCAGATTCATATGTGGTAAGTTTCTTAGTTCTGAGTTCGTAATTCATAGTCGTTAACAAGATATAGATGTACAAGCTAGGTATATGTTCGAGTAGACAAGTTTTTAGTCTCTATATTTCTAGCTCTGGTTCATATCGGTCACTGTCATTCAAAACGTTAATTTTTGTTATTGTATCCTCTAGCACTGGTTCATTTTGGTTTAGGTACCTTTAAATATTCATTTTGGTTTGGATCGGTGGAATCTCGTAAATAGAAATTTATGATTATGATTTAAA

mRNA sequence

TGTAACCAAGTAGAGAACTGATTGCTTAAGCTTTAAACCCTAGTCCCGTCGCCGCCGTCTTAACTCAAGTCTCCGGCGACAGTTTGCGTTCAGAATCTTCCCCACTACCAGTGAAAATCTCCATTCTGATTTTGTTTGTTCCCAGCGAAAATCTCCGAGCTTATTCATCTATTTTCAGTTTTTTTTATTGCCGGAGGACGATAATTGTTCTATTCTGATCTGAAATTCTTATTGAGTTCGGTGTTTTTTTTTTTTGTTGTACTATCGAGTATTGAGGAAAATCGAACTTGTAAATCGACCAATGTTGGACGAAAGCAAATTCGATGTGCATTTGAAACTCTGGGCGCTAAGAATTCCCAGGGAACTCTGTAAATCCGCCATTAAAATACTGAACGGGTACTTAATTGATAGGCCCCGGATTAAACCTGTGACGGAAGATCCAACATGTGATAAGAATCGGTATGTTATATTATCGGAAAAAGTTCAATCTACAGAGTTGTCCGAAATTCCAGAAATGAAGCTTGAAGAGCTGAAAGGTTTATGCGAGATTGAACTAGTTCCTTATTCACTAACTCTTGGATATTCTTATTGGGGAACAGATCATGTACTGAAGCAGATTCTGCCTCATGGAGTGGAAGTGCCATCATCTTTTGAAACAATACTTTTACGTGCATTCTTTTCTTCTCCTATATGGCTTTTAGGTCACGTAGCTCACCTTAATATACACGACGAATTGCTCCCTTATAAGGAAGTCATTGCGAAGGTTATTTATGATAAAAATTATCCGAGAATTAAAACAGTGGTAAATAAGGTGGGGTCGATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAGTTCTGAAGGGAGAGAATGATATGGAAACAGAAGTGAAACAATATGGAGCAACTTTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGGTTAGAGCATGAACACATAAGACTGGTTTCACTGTTCCAACCAGGAGAGATAATTTGTGACATGTTCGCCGGTATTGGTCCTTTTGCTATTCCAGCTGCCCAGAAAGGCTGTATAGTATATGCCAATGACTTGAACCCGGATAGTGTTCGATATCTGAAGATTAATGCAGAAATCAATAAAGTCGGTGATCGTGTTCATGTTCATAATTTGGACGCTAGGAAGTTCATTTCTCAATTGATGACCGTGCCAGACAATGAATGTAATTCTGAATCTGTTGCCTCGGTGTTAAAAGCTTGTGAGAAAGTTCAGGTGGAGGAAGTCCATGGTAGTGTTAAAACTAGCGATGAAAGTGTAGAAGGCTCAAGGAGTGCTGATGTATCTGTTGCTGCTCTTAAAAGATCCTCAGATTGCTGTGAGAAAGATAATGGAATAGCTGATGATGCTTCCATCTGTGTAGCTGGTAAGAGAAAAGGAAACAGTAACAAGAGGTTGAAGGGGTCCGAAATATCTCATGCTAAGACCTGGGAGCATGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCCTTACAGTTTCTCGATGCATTTAGGGGCTCGATTCCGCTGAAGTACTGGAAGGGCTCCCTCCCTTGGATTCACTGTTATTGCTTCATGCGAGCAAATGAGAATCAAGAGTTCATCATTTCGGTGGCCGAGTCTGCCTTGAATGCTAAAATCCAAGACCCTATTTTTCATAGAGTCAGAGATGTTGCACCAAACAAGGCAATGTATTGTTTAAGCTTTAGGCTTCCACAACAATGCTTCAACGAAGAGTTAGCTAGCTGAATCGTATCCGTTGCAGTCGGTCGGGATGCTTACTAAGCCAAGTTTTCAGATTCATATGTGGTAAGTTTCTTAGTTCTGAGTTCGTAATTCATAGTCGTTAACAAGATATAGATGTACAAGCTAGGTATATGTTCGAGTAGACAAGTTTTTAGTCTCTATATTTCTAGCTCTGGTTCATATCGGTCACTGTCATTCAAAACGTTAATTTTTGTTATTGTATCCTCTAGCACTGGTTCATTTTGGTTTAGGTACCTTTAAATATTCATTTTGGTTTGGATCGGTGGAATCTCGTAAATAGAAATTTATGATTATGATTTAAA

Coding sequence (CDS)

ATGTTGGACGAAAGCAAATTCGATGTGCATTTGAAACTCTGGGCGCTAAGAATTCCCAGGGAACTCTGTAAATCCGCCATTAAAATACTGAACGGGTACTTAATTGATAGGCCCCGGATTAAACCTGTGACGGAAGATCCAACATGTGATAAGAATCGGTATGTTATATTATCGGAAAAAGTTCAATCTACAGAGTTGTCCGAAATTCCAGAAATGAAGCTTGAAGAGCTGAAAGGTTTATGCGAGATTGAACTAGTTCCTTATTCACTAACTCTTGGATATTCTTATTGGGGAACAGATCATGTACTGAAGCAGATTCTGCCTCATGGAGTGGAAGTGCCATCATCTTTTGAAACAATACTTTTACGTGCATTCTTTTCTTCTCCTATATGGCTTTTAGGTCACGTAGCTCACCTTAATATACACGACGAATTGCTCCCTTATAAGGAAGTCATTGCGAAGGTTATTTATGATAAAAATTATCCGAGAATTAAAACAGTGGTAAATAAGGTGGGGTCGATAACAAATGAGTTTCGGGTTCCAAAGTTTGAAGTTCTGAAGGGAGAGAATGATATGGAAACAGAAGTGAAACAATATGGAGCAACTTTTAAGCTTGACTACAGTTTGGTCTACTGGAACTCAAGGTTAGAGCATGAACACATAAGACTGGTTTCACTGTTCCAACCAGGAGAGATAATTTGTGACATGTTCGCCGGTATTGGTCCTTTTGCTATTCCAGCTGCCCAGAAAGGCTGTATAGTATATGCCAATGACTTGAACCCGGATAGTGTTCGATATCTGAAGATTAATGCAGAAATCAATAAAGTCGGTGATCGTGTTCATGTTCATAATTTGGACGCTAGGAAGTTCATTTCTCAATTGATGACCGTGCCAGACAATGAATGTAATTCTGAATCTGTTGCCTCGGTGTTAAAAGCTTGTGAGAAAGTTCAGGTGGAGGAAGTCCATGGTAGTGTTAAAACTAGCGATGAAAGTGTAGAAGGCTCAAGGAGTGCTGATGTATCTGTTGCTGCTCTTAAAAGATCCTCAGATTGCTGTGAGAAAGATAATGGAATAGCTGATGATGCTTCCATCTGTGTAGCTGGTAAGAGAAAAGGAAACAGTAACAAGAGGTTGAAGGGGTCCGAAATATCTCATGCTAAGACCTGGGAGCATGTTGATCATGTGATTATGAACCTCCCTGCTTCTGCCTTACAGTTTCTCGATGCATTTAGGGGCTCGATTCCGCTGAAGTACTGGAAGGGCTCCCTCCCTTGGATTCACTGTTATTGCTTCATGCGAGCAAATGAGAATCAAGAGTTCATCATTTCGGTGGCCGAGTCTGCCTTGAATGCTAAAATCCAAGACCCTATTTTTCATAGAGTCAGAGATGTTGCACCAAACAAGGCAATGTATTGTTTAAGCTTTAGGCTTCCACAACAATGCTTCAACGAAGAGTTAGCTAGCTGA

Protein sequence

MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEKVQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETILLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVPKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIGPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDNECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIADDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQQCFNEELAS

Homology

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BLAST of CmaCh12G012350 vs. ExPASy Swiss-Prot
Match: F6HH45 (tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0016g04350 PE=3 SV=1)

HSP 1 Score: 686.8 bits (1771), Expect = 1.8e-196
Identity = 344/506 (67.98%), Postives = 408/506 (80.63%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           +LDESKFD+ LKLWALRIP +LCK A +ILNGYL+D+PR+KP+TEDPTC+ NRY+ILSE+
Sbjct: 2   LLDESKFDLQLKLWALRIPSQLCKVASRILNGYLLDKPRVKPITEDPTCETNRYIILSER 61

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           +++ +LS+IPE K +ELK LC+IE+VPYSLTLGYSYW  DHVLKQILP GVEVPSSFETI
Sbjct: 62  IKNPDLSDIPEDKRDELKVLCKIEVVPYSLTLGYSYWSADHVLKQILPDGVEVPSSFETI 121

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                     + L H+AHLNI  ELLPYK+VIAKVIYDKNYPRIKTVVNKVG+ITNEFRV
Sbjct: 122 ---------TFSLCHIAHLNITGELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNEFRV 181

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFE+L G++DM TEVKQY ATFKLDYSLVYWNSRLEHEH+RLVS F+PG+IICDMF+G+
Sbjct: 182 PKFEILVGKDDMVTEVKQYRATFKLDYSLVYWNSRLEHEHMRLVSQFRPGQIICDMFSGV 241

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGC+VYANDLNPDS+RYLKINA+INKV D +  +N+DARKFISQLM VP +
Sbjct: 242 GPFAIPAAQKGCLVYANDLNPDSIRYLKINAKINKVDDNIWAYNMDARKFISQLMEVPVH 301

Query: 301 ECNSESVASVLKACEK------VQVEE-----------VHGSVKTSDESVEGSRS-ADVS 360
           E   E    VLKA E+      VQ E            VH    ++ + +EGS + AD +
Sbjct: 302 EILPEPDVPVLKATEECSIQANVQTESQNGGLTVEAIGVHSDDSSNMDGIEGSCTVADDT 361

Query: 361 VAALKRSSDCCEKDNGIADDASICVAGKRKGNSNKRLKGSE-ISHAKTWEHVDHVIMNLP 420
           VAA+K+ S  CE++NG  D+A++ V G+RK   NKR++GSE  S+ K WEHVDHVIMNLP
Sbjct: 362 VAAVKKHSGSCEEENGTYDNATVSVVGRRKDRMNKRMRGSESSSNIKPWEHVDHVIMNLP 421

Query: 421 ASALQFLDAFRGSIPLKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHR 480
           ASA+QFLDAFRG I  KYWKGSLPWIHCYCF+RANE  E IIS AE+ALNA IQ+PI HR
Sbjct: 422 ASAIQFLDAFRGLIQRKYWKGSLPWIHCYCFIRANETNEMIISEAETALNASIQEPILHR 481

Query: 481 VRDVAPNKAMYCLSFRLPQQ-CFNEE 487
           VRDVAPNKAM+CLSFRL ++ CF E+
Sbjct: 482 VRDVAPNKAMFCLSFRLSEEACFKED 498

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BLAST of CmaCh12G012350 vs. ExPASy Swiss-Prot
Match: Q93YU6 (tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3g56120 PE=2 SV=1)

HSP 1 Score: 641.0 bits (1652), Expect = 1.1e-182
Identity = 318/487 (65.30%), Postives = 378/487 (77.62%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+LKLWALRIPRELCKSA +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           V++ +LSEIPE KL +LK L E+E+VP+S+TLGYSYW  DH+LKQILP G+++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GH+AHLN+HDELLP+K+VIAKVIYDKNYPRIKT+VNKVG+I+NEFRV
Sbjct: 121 -------------GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVL GEN METEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGI
Sbjct: 181 PKFEVLAGENGMETEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGC VYANDLNPDSVRYLKINA+ NKV D + VHN+DARKF S LM V   
Sbjct: 241 GPFAIPAAQKGCFVYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTC 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESV-EGSRSADVSVAALKRSSDCCEKDNGI 360
           E N +SVA   K   K       G   +S E + E + S +  + A K+ S   + +NG+
Sbjct: 301 EDNLQSVADNDKT--KEAAVSRGGETNSSGEEIRESNASINEPLGANKKPSGTTKTENGV 360

Query: 361 ADDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKY 420
             D         +G++NKRL+ + +  AK WEH+DHVIMNLPASALQFLD+F   I  KY
Sbjct: 361 GKDCK-----SIEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQKKY 420

Query: 421 WKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLP 480
           WKG LP IHCYCF+RA+E  EFII+ AE+AL   I+DP+FH+VRDVAPNKAM+CLSFRLP
Sbjct: 421 WKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSFRLP 467

Query: 481 QQCFNEE 487
           + C  +E
Sbjct: 481 EACLKQE 467

[Close]

BLAST of CmaCh12G012350 vs. ExPASy Swiss-Prot
Match: C5XX79 (tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g025390 PE=3 SV=1)

HSP 1 Score: 551.2 bits (1419), Expect = 1.2e-155
Identity = 285/495 (57.58%), Postives = 350/495 (70.71%), Query Frame = 0


Query: 2   LDESKFDVHLKLWALRIPRELCKSAIKIL-NGYLIDRPRIKPVTEDPTCDKNRYVILSEK 61
           LDESKF+  L+LWALRIPREL  +  ++L +GYL+D+PR+K V EDP  DKNR VILSEK
Sbjct: 4   LDESKFEQRLQLWALRIPRELSSAVTRLLRSGYLLDKPRVKTVVEDPEGDKNRLVILSEK 63

Query: 62  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 121
           +Q  +LS++P+ +L+ LK LC +++VPY+LTLGYSYW                       
Sbjct: 64  IQKPDLSDMPQQELDSLKQLCNVDVVPYTLTLGYSYWSA--------------------- 123

Query: 122 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 181
                        GHVAHLNI D+LLPYK VIAKVIYDKNYPRI+TV NKVG+ITNEFRV
Sbjct: 124 -------------GHVAHLNISDDLLPYKNVIAKVIYDKNYPRIQTVANKVGTITNEFRV 183

Query: 182 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 241
           PKFE+L G+NDM TEVKQYGATFKLDY LVYWNSRL+HEHIRLVSLF+ G++ICDMFAGI
Sbjct: 184 PKFEILAGKNDMVTEVKQYGATFKLDYGLVYWNSRLDHEHIRLVSLFKKGDVICDMFAGI 243

Query: 242 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 301
           GPFAIPAAQKGC+VYANDLNPDSV YL+ NA+INKV D +  +N+DAR F+  LMTVP  
Sbjct: 244 GPFAIPAAQKGCVVYANDLNPDSVHYLRTNAKINKVDDYIFAYNMDARVFMQNLMTVPGL 303

Query: 302 ECNSESVASVLKACEK--VQVEEVHGSVKTSDESVEGSR-----SADVSVAALKR--SSD 361
           E  S+   +  ++  K  V   E   S    ++  EGS+     S+  S  A KR  +S+
Sbjct: 304 ETGSDCQVAADESYPKEGVPANENSSSNGNHNDVREGSQNGANESSVASTTAKKRQQTSE 363

Query: 362 CCEKDNGIADDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAF 421
            CE D    D      A + K  +NKR++G     +K WEH DHV+MNLPASALQFLD F
Sbjct: 364 ECESDCQDGD------ASQTKRRNNKRVRGPGPPPSKPWEHFDHVLMNLPASALQFLDCF 423

Query: 422 RGSIPLKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAM 481
            G +  KYW GSLPWIHCYCF+R++E++E I+S   + LNAKI +PIFHRVRDVAPNKAM
Sbjct: 424 DGLVQKKYWTGSLPWIHCYCFIRSSESEESILS---NKLNAKIAEPIFHRVRDVAPNKAM 455

Query: 482 YCLSFRLPQQCFNEE 487
           +CLSF+LP +C  E+
Sbjct: 484 FCLSFKLPMECLRED 455

[Close]

BLAST of CmaCh12G012350 vs. ExPASy Swiss-Prot
Match: A9T6G5 (tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPADRAFT_141055 PE=3 SV=1)

HSP 1 Score: 425.2 bits (1092), Expect = 9.7e-118
Identity = 220/469 (46.91%), Postives = 302/469 (64.39%), Query Frame = 0


Query: 2   LDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEKV 61
           +D+S F V L   ALRIP++       +L+ YL+D PR+K + EDP     R ++ SE +
Sbjct: 11  IDKSAFVVRLPRVALRIPKQKSHRVASLLSKYLLDMPRVKHIVEDPQDPSMRLLLFSEAI 70

Query: 62  QSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETIL 121
              +L  + E K+  +K +  +++V + + L YSYW  +H+LK+ILP G EVPSSFETI 
Sbjct: 71  AQPDLEGVSEEKILAVKEIVPLDVVQHEVVLDYSYWPVEHILKEILPAGCEVPSSFETI- 130

Query: 122 LRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVP 181
                       GH+AHLN+ ++LL YK++IA+VI DKN P++KTVVNKVG+ITNEFRVP
Sbjct: 131 ------------GHIAHLNLREDLLTYKKIIAEVILDKN-PKLKTVVNKVGTITNEFRVP 190

Query: 182 KFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIG 241
           +FE+L GE  + TE+KQ+GATF+LDY +VYWNSRLE EH RL + F+PG++I DMFAGIG
Sbjct: 191 EFEILAGEPSLVTEIKQHGATFRLDYGMVYWNSRLEGEHKRLFAQFKPGQVIVDMFAGIG 250

Query: 242 PFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDNE 301
           PFAIPAAQ+GC VYANDLNP SV++LK+N++INKVG+ +   NLDAR+F+ +L+T  +  
Sbjct: 251 PFAIPAAQQGCAVYANDLNPTSVKFLKLNSDINKVGESIKAFNLDAREFMRKLVT-EEEG 310

Query: 302 CNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIAD 361
           C  E  +S L A   +    +  S + S    EGS      + + K+  +   K   +++
Sbjct: 311 CVGEIASSTLIAENSLNGTRIPSSKENSSSLTEGS-----DMPSSKKPEERKAKKAKVSE 370

Query: 362 DASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYWK 421
              +                 +IS  K WEH DHV+MNLPASAL+FLD   G +    WK
Sbjct: 371 VVKM-----------------KISDVKPWEHFDHVVMNLPASALEFLDVLNGLLCKGRWK 430

Query: 422 GSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKA 471
           G++P +HCYCFMR+NE    I+  AE+ L   I DP  + VRDVAPNKA
Sbjct: 431 GTMPRVHCYCFMRSNETNADIVKKAETYLGGSIADPDVYVVRDVAPNKA 442

[Close]

BLAST of CmaCh12G012350 vs. ExPASy Swiss-Prot
Match: F4NUJ6 (tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strain JAM81 / FGSC 10211) OX=684364 GN=TRM5 PE=3 SV=1)

HSP 1 Score: 286.6 bits (732), Expect = 5.4e-76
Identity = 171/484 (35.33%), Postives = 262/484 (54.13%), Query Frame = 0


Query: 2   LDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEKV 61
           L+ + F+    L AL+IP      A++ L  +++  PR++ + +DPT  K R ++L   +
Sbjct: 16  LNHALFESSHTLPALKIPAHASGIAMETLRSHILVAPRLRTIVDDPTDKKWRLLLLDPSM 75

Query: 62  QSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETIL 121
           +++E++++P   L+E     E +LV +++ L Y YW +D VL+ ILP  +E P +FET+ 
Sbjct: 76  EASEINDLPN-PLKEFALKHEAKLVKHTIELKYDYWTSDQVLRSILPDEMETPGAFETV- 135

Query: 122 LRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRVP 181
                       GH+AHLN+ D   P+K +I +VI DK+   IKTVVNK+ +I + FR  
Sbjct: 136 ------------GHIAHLNLRDRYQPFKHIIGQVILDKS-SHIKTVVNKLDNIDHTFRFF 195

Query: 182 KFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGIG 241
           + E+L G NDM  ++K+ G  F  D+S VYWNSRL+ EH R++ LF   ++ICD+FAG+G
Sbjct: 196 QMEILAGINDMNAKLKEGGCFFHFDFSKVYWNSRLQGEHDRIIKLFGQNDLICDVFAGVG 255

Query: 242 PFAIPAAQ-KGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 301
           PFA+PAA+ K C+V+ANDLNP S +YL  N ++NK+  R+   N+D R+FI Q +     
Sbjct: 256 PFALPAAKHKRCVVFANDLNPQSFKYLMENIKLNKLETRILPFNMDGRQFIKQSL----E 315

Query: 302 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 361
           + N+ ++        K+  ++   + K  +  VE    A                   + 
Sbjct: 316 DLNNPAI------WNKITKQKPTSNDKKRNRKVESPTVAP------------------LT 375

Query: 362 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRG---SIPL 421
           D  +I                S I H K      H +MNLPA+A++FLDAF G    +  
Sbjct: 376 DQPAI----------------SGIRHFK------HYVMNLPATAIEFLDAFHGLYSGMRD 434

Query: 422 KYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIF--HRVRDVAPNKAMYCLS 480
                 LP IHC+CF  A + +  +I   E  +   +   +   H VR VAPNK M C+S
Sbjct: 436 VIMDSDLPTIHCHCFSNAKDVKADVIERVERVIGMPLGSNLIMVHSVRTVAPNKDMLCIS 434

[Close]

BLAST of CmaCh12G012350 vs. ExPASy TrEMBL
Match: A0A6J1HME6 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111465526 PE=3 SV=1)

HSP 1 Score: 960.7 bits (2482), Expect = 2.4e-276
Identity = 476/489 (97.34%), Postives = 476/489 (97.34%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV
Sbjct: 121 -------------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI
Sbjct: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN
Sbjct: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360
           ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA
Sbjct: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360

Query: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420
           DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW
Sbjct: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420

Query: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ 480
           KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ
Sbjct: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ 476

Query: 481 QCFNEELAS 490
           QCFNEELAS
Sbjct: 481 QCFNEELAS 476

[Close]

BLAST of CmaCh12G012350 vs. ExPASy TrEMBL
Match: A0A6J1FG25 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443851 PE=3 SV=1)

HSP 1 Score: 949.9 bits (2454), Expect = 4.2e-273
Identity = 471/489 (96.32%), Postives = 473/489 (96.73%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV
Sbjct: 121 -------------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI
Sbjct: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN
Sbjct: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360
           ECNSESVASVLKACEKVQVEEV GSVKTSDESVEGSRSADVSVAALKRSS+CCEKDNGIA
Sbjct: 301 ECNSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKDNGIA 360

Query: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420
           DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASAL FLDAFRGSIP KYW
Sbjct: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALHFLDAFRGSIPRKYW 420

Query: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ 480
           KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ
Sbjct: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ 476

Query: 481 QCFNEELAS 490
           QCF+EELAS
Sbjct: 481 QCFDEELAS 476

[Close]

BLAST of CmaCh12G012350 vs. ExPASy TrEMBL
Match: A0A6J1HPS1 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111465526 PE=3 SV=1)

HSP 1 Score: 918.3 bits (2372), Expect = 1.3e-263
Identity = 456/469 (97.23%), Postives = 456/469 (97.23%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV
Sbjct: 121 -------------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI
Sbjct: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN
Sbjct: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360
           ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA
Sbjct: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360

Query: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420
           DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW
Sbjct: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420

Query: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 470
           KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK
Sbjct: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 456

[Close]

BLAST of CmaCh12G012350 vs. ExPASy TrEMBL
Match: A0A6J1FGN5 (tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111443851 PE=3 SV=1)

HSP 1 Score: 909.4 bits (2349), Expect = 6.3e-261
Identity = 452/469 (96.38%), Postives = 453/469 (96.59%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV
Sbjct: 121 -------------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI
Sbjct: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN
Sbjct: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360
           ECNSESVASVLKACEKVQVEEV GSVKTSDESVEGSRSADVSVAALKRSS+CCEKDNGIA
Sbjct: 301 ECNSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKDNGIA 360

Query: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420
           DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASAL FLDAFRGSIP KYW
Sbjct: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALHFLDAFRGSIPRKYW 420

Query: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 470
           KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK
Sbjct: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 456

[Close]

BLAST of CmaCh12G012350 vs. ExPASy TrEMBL
Match: A0A6J1DUW2 (tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111024249 PE=3 SV=1)

HSP 1 Score: 833.6 bits (2152), Expect = 4.4e-238
Identity = 411/498 (82.53%), Postives = 441/498 (88.55%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCK+AIKILNGYLIDR R+KPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKAAIKILNGYLIDRARVKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQ+ ELS+IPE KLEELKGLCEIELVPYSLTLGYSYWG DH+LKQILP  VEVPSSFETI
Sbjct: 61  VQAPELSQIPEAKLEELKGLCEIELVPYSLTLGYSYWGADHILKQILPPRVEVPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GH+AHLNIHDELLPYK+VIAKVIYDKNYPRIKTVVNKVG+ITN+FRV
Sbjct: 121 -------------GHIAHLNIHDELLPYKDVIAKVIYDKNYPRIKTVVNKVGTITNKFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFE+L GE+DM TEVKQYGATFKLDY LVYWNSRLEHEH+RLVSLFQPGEIICDMFAGI
Sbjct: 181 PKFEILMGEHDMVTEVKQYGATFKLDYGLVYWNSRLEHEHVRLVSLFQPGEIICDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQK CIVY NDLNPDSV+YLK+NAEINKV DRVHV+NLDARKFISQLM VPDN
Sbjct: 241 GPFAIPAAQKECIVYGNDLNPDSVQYLKVNAEINKVSDRVHVYNLDARKFISQLMIVPDN 300

Query: 301 ECNSESVASVLKACEK-------------VQVEEVHGSVKTSDESVEGSRSADVSVAALK 360
           E NSESV SVLKAC+K             VQVEEV  + K SDE+VE SRSAD+SV+ LK
Sbjct: 301 ESNSESVTSVLKACDKGIGGSNQESPSANVQVEEVQENSKISDETVEHSRSADISVSVLK 360

Query: 361 RSSDCCEKDNGIADDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQF 420
           RSS+ CEKD+GI DDASI VAG++K N+NKR+KGS I HA+TWEHVDHVIMNLPASALQF
Sbjct: 361 RSSESCEKDDGIVDDASISVAGRKKRNANKRMKGSNICHARTWEHVDHVIMNLPASALQF 420

Query: 421 LDAFRGSIPLKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAP 480
           LDAFRGSI  KYWKGSLPWIHCYCFMRANENQ+FIIS AESALN KIQDP+FHRVRDVAP
Sbjct: 421 LDAFRGSIRRKYWKGSLPWIHCYCFMRANENQDFIISEAESALNGKIQDPVFHRVRDVAP 480

Query: 481 NKAMYCLSFRLPQQCFNE 486
           NKAMYCLSFRLP+QCFNE
Sbjct: 481 NKAMYCLSFRLPEQCFNE 485

[Close]

BLAST of CmaCh12G012350 vs. NCBI nr
Match: XP_022965706.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita maxima] >XP_022965707.1 tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita maxima])

HSP 1 Score: 960.7 bits (2482), Expect = 4.9e-276
Identity = 476/489 (97.34%), Postives = 476/489 (97.34%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV
Sbjct: 121 -------------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI
Sbjct: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN
Sbjct: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360
           ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA
Sbjct: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360

Query: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420
           DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW
Sbjct: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420

Query: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ 480
           KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ
Sbjct: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ 476

Query: 481 QCFNEELAS 490
           QCFNEELAS
Sbjct: 481 QCFNEELAS 476

[Close]

BLAST of CmaCh12G012350 vs. NCBI nr
Match: XP_022937443.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita moschata] >XP_022937444.1 tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita moschata])

HSP 1 Score: 949.9 bits (2454), Expect = 8.6e-273
Identity = 471/489 (96.32%), Postives = 473/489 (96.73%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV
Sbjct: 121 -------------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI
Sbjct: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN
Sbjct: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360
           ECNSESVASVLKACEKVQVEEV GSVKTSDESVEGSRSADVSVAALKRSS+CCEKDNGIA
Sbjct: 301 ECNSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKDNGIA 360

Query: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420
           DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASAL FLDAFRGSIP KYW
Sbjct: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALHFLDAFRGSIPRKYW 420

Query: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ 480
           KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ
Sbjct: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ 476

Query: 481 QCFNEELAS 490
           QCF+EELAS
Sbjct: 481 QCFDEELAS 476

[Close]

BLAST of CmaCh12G012350 vs. NCBI nr
Match: XP_023537242.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 938.3 bits (2424), Expect = 2.6e-269
Identity = 465/489 (95.09%), Postives = 469/489 (95.91%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDES+FDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESEFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV
Sbjct: 121 -------------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI
Sbjct: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGCIVYANDLNPDS+RYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN
Sbjct: 241 GPFAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360
           ECNSESVASVLKACEKVQVEEV GSVKTSDESVEGSRSADVSVAALKRSS+CCEK  GIA
Sbjct: 301 ECNSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKGKGIA 360

Query: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420
           DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASAL FLDAFR SIP KYW
Sbjct: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALHFLDAFRRSIPRKYW 420

Query: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ 480
           KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ
Sbjct: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLPQ 476

Query: 481 QCFNEELAS 490
           QCF+EEL S
Sbjct: 481 QCFDEELTS 476

[Close]

BLAST of CmaCh12G012350 vs. NCBI nr
Match: XP_023537240.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023537241.1 tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 934.1 bits (2413), Expect = 4.9e-268
Identity = 467/502 (93.03%), Postives = 471/502 (93.82%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDES+FDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESEFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV
Sbjct: 121 -------------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI
Sbjct: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGCIVYANDLNPDS+RYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN
Sbjct: 241 GPFAIPAAQKGCIVYANDLNPDSIRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDN--- 360
           ECNSESVASVLKACEKVQVEEV GSVKTSDESVEGSRSADVSVAALKRSS+CCEKDN   
Sbjct: 301 ECNSESVASVLKACEKVQVEEVQGSVKTSDESVEGSRSADVSVAALKRSSECCEKDNGIA 360

Query: 361 ----------GIADDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQF 420
                     GIADDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASAL F
Sbjct: 361 DDASICVAGKGIADDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALHF 420

Query: 421 LDAFRGSIPLKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAP 480
           LDAFR SIP KYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAP
Sbjct: 421 LDAFRRSIPRKYWKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAP 480

Query: 481 NKAMYCLSFRLPQQCFNEELAS 490
           NKAMYCLSFRLPQQCF+EEL S
Sbjct: 481 NKAMYCLSFRLPQQCFDEELTS 489

[Close]

BLAST of CmaCh12G012350 vs. NCBI nr
Match: XP_022965708.1 (tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucurbita maxima])

HSP 1 Score: 918.3 bits (2372), Expect = 2.8e-263
Identity = 456/469 (97.23%), Postives = 456/469 (97.23%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK
Sbjct: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI
Sbjct: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV
Sbjct: 121 -------------GHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI
Sbjct: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN
Sbjct: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360
           ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA
Sbjct: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAALKRSSDCCEKDNGIA 360

Query: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420
           DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW
Sbjct: 361 DDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKYW 420

Query: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 470
           KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK
Sbjct: 421 KGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNK 456

[Close]

BLAST of CmaCh12G012350 vs. TAIR 10
Match: AT3G56120.1 (S-adenosyl-L-methionine-dependent methyltransferases superfamily protein )

HSP 1 Score: 641.0 bits (1652), Expect = 8.0e-184
Identity = 318/487 (65.30%), Postives = 378/487 (77.62%), Query Frame = 0


Query: 1   MLDESKFDVHLKLWALRIPRELCKSAIKILNGYLIDRPRIKPVTEDPTCDKNRYVILSEK 60
           M DESKFDV+LKLWALRIPRELCKSA +ILNGY+++ PRIKP+TEDPTC+K R VILSE 
Sbjct: 1   MFDESKFDVNLKLWALRIPRELCKSASRILNGYMLNMPRIKPITEDPTCEKTRLVILSES 60

Query: 61  VQSTELSEIPEMKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQILPHGVEVPSSFETI 120
           V++ +LSEIPE KL +LK L E+E+VP+S+TLGYSYW  DH+LKQILP G+++PSSFETI
Sbjct: 61  VKNADLSEIPEEKLNQLKKLSELEVVPHSVTLGYSYWSADHLLKQILPDGLDIPSSFETI 120

Query: 121 LLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKTVVNKVGSITNEFRV 180
                        GH+AHLN+HDELLP+K+VIAKVIYDKNYPRIKT+VNKVG+I+NEFRV
Sbjct: 121 -------------GHIAHLNLHDELLPFKDVIAKVIYDKNYPRIKTIVNKVGTISNEFRV 180

Query: 181 PKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICDMFAGI 240
           PKFEVL GEN METEVKQYGA FKLDY LVYWNSRLEHEH+RL SLF+PGE +CDMFAGI
Sbjct: 181 PKFEVLAGENGMETEVKQYGARFKLDYGLVYWNSRLEHEHMRLSSLFKPGETVCDMFAGI 240

Query: 241 GPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLDARKFISQLMTVPDN 300
           GPFAIPAAQKGC VYANDLNPDSVRYLKINA+ NKV D + VHN+DARKF S LM V   
Sbjct: 241 GPFAIPAAQKGCFVYANDLNPDSVRYLKINAKFNKVDDLICVHNMDARKFFSHLMAVSTC 300

Query: 301 ECNSESVASVLKACEKVQVEEVHGSVKTSDESV-EGSRSADVSVAALKRSSDCCEKDNGI 360
           E N +SVA   K   K       G   +S E + E + S +  + A K+ S   + +NG+
Sbjct: 301 EDNLQSVADNDKT--KEAAVSRGGETNSSGEEIRESNASINEPLGANKKPSGTTKTENGV 360

Query: 361 ADDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQFLDAFRGSIPLKY 420
             D         +G++NKRL+ + +  AK WEH+DHVIMNLPASALQFLD+F   I  KY
Sbjct: 361 GKDCK-----SIEGHANKRLRQTLLPIAKPWEHIDHVIMNLPASALQFLDSFSNVIQKKY 420

Query: 421 WKGSLPWIHCYCFMRANENQEFIISVAESALNAKIQDPIFHRVRDVAPNKAMYCLSFRLP 480
           WKG LP IHCYCF+RA+E  EFII+ AE+AL   I+DP+FH+VRDVAPNKAM+CLSFRLP
Sbjct: 421 WKGPLPLIHCYCFIRASETTEFIIAEAETALKFHIEDPVFHKVRDVAPNKAMFCLSFRLP 467

Query: 481 QQCFNEE 487
           + C  +E
Sbjct: 481 EACLKQE 467

[Close]

BLAST of CmaCh12G012350 vs. TAIR 10
Match: AT4G27340.1 (Met-10+ like family protein )

HSP 1 Score: 214.2 bits (544), Expect = 2.4e-55
Identity = 140/441 (31.75%), Postives = 209/441 (47.39%), Query Frame = 0


Query: 53  RYVILSEKVQSTELSEIPE------MKLEELKGLCEIELVPYSLTLGYSYWGTDHVLKQI 112
           R ++L EK    E+ ++PE       + +        ELV   LTL Y YW    +L+ +
Sbjct: 274 RLLLLDEKYSGEEVQDLPEAIKVLFAEAKMADASLSFELVKCRLTLFYDYWPMIEILEAV 333

Query: 113 LPHGVEVPSSFETILLRAFFSSPIWLLGHVAHLNIHDELLPYKEVIAKVIYDKNYPRIKT 172
           LP G+ VPS+FE             ++GH+AHLN+ DE LPYK +IAKV+ DKN P+I+T
Sbjct: 334 LPKGMIVPSAFE-------------MVGHIAHLNLRDEHLPYKRLIAKVVLDKNQPKIQT 393

Query: 173 VVNKVGSITNEFRVPKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSL 232
           VVNK+  I N+FR  + EVL G + + T V + G  F +D + VYWNS+L  E  RL+  
Sbjct: 394 VVNKIDPIHNDFRTMQLEVLAGNHSLVTLVVENGLRFHVDLARVYWNSKLGTERQRLLLG 453

Query: 233 FQPGEIICDMFAGIGPFAIPAAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHVHNLD 292
           F   +++CD+FAG+GP A+ AA+    VYANDLNP +V +++ N+ +NK+  R+ + N+D
Sbjct: 454 FDQNDVVCDVFAGVGPIALAAARIVKRVYANDLNPHAVEFMEQNSVVNKLEKRIEIFNMD 513

Query: 293 ARKFISQLMTVPDNECNSESVASVLKACEKVQVEEVHGSVKTSDESVEGSRSADVSVAAL 352
            R+FI  + +                                                  
Sbjct: 514 GRRFIKAMFS-------------------------------------------------- 573

Query: 353 KRSSDCCEKDNGIADDASICVAGKRKGNSNKRLKGSEISHAKTWEHVDHVIMNLPASALQ 412
                                    KG                 + V  V+MNLP  A +
Sbjct: 574 -----------------------SEKG-----------------QKVTQVVMNLPKDAAE 606

Query: 413 FLDAFRGSIPLKYWKG--SLPWIHCYCFMRANE-----NQEFIISVAESALNAKIQDPIF 472
            LDAFRG    ++     S P IH Y F +A++     ++   I+++E A++ K++    
Sbjct: 634 SLDAFRGVYNDRHRDEGLSFPTIHVYGFSKASDPEFDFHERIRIALSEVAVDVKMR---- 606

Query: 473 HRVRDVAPNKAMYCLSFRLPQ 481
            +VR VAP K M C SF LP+
Sbjct: 694 -KVRLVAPGKWMLCASFILPK 606

[Close]

BLAST of CmaCh12G012350 vs. TAIR 10
Match: AT4G04670.1 (Met-10+ like family protein / kelch repeat-containing protein )

HSP 1 Score: 59.3 bits (142), Expect = 9.9e-09
Identity = 47/169 (27.81%), Postives = 82/169 (48.52%), Query Frame = 0


Query: 176 NEFRVPKFEVLKGENDMETEVKQYGATFKLDYSLVYWNSRLEHEHIRLVSLFQPGEIICD 235
           N  R    E+L G+N      ++ G  +  D +   ++     E +R+ ++    E++ D
Sbjct: 785 NGTRDSTLEILVGDNGWVNH-RENGILYSFDATKCMFSWGNLSEKLRMGNMACENEVVVD 844

Query: 236 MFAGIGPFAIP--AAQKGCIVYANDLNPDSVRYLKINAEINKVGDRVHV----HNLDARK 295
           +FAGIG F +P     K  +VYA + NP ++  L+ N E N V +R  +    + + A K
Sbjct: 845 LFAGIGYFVLPFLVRAKAKLVYACEWNPHAIEALRRNVEANSVSERCIILEGDNRITAPK 904

Query: 296 FIS---QLMTVPDNECNSESVASVLKACEKVQVEEVHGSVKTSDESVEG 336
            ++    L  +P +E +  +    L+   +  +  VHG+VK SDES  G
Sbjct: 905 GVADRVNLGLIPSSEGSWVTAIQALR--PEGGILHVHGNVKDSDESSWG 950

The following BLAST results are available for this feature:

BLAST of CmaCh12G012350 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
F6HH45	1.8e-196	67.98	tRNA (guanine(37)-N1)-methyltransferase 2 OS=Vitis vinifera OX=29760 GN=VIT_11s0...	[more]
Q93YU6	1.1e-182	65.30	tRNA (guanine(37)-N1)-methyltransferase 1 OS=Arabidopsis thaliana OX=3702 GN=At3...	[more]
C5XX79	1.2e-155	57.58	tRNA (guanine(37)-N1)-methyltransferase OS=Sorghum bicolor OX=4558 GN=Sb04g02539...	[more]
A9T6G5	9.7e-118	46.91	tRNA (guanine(37)-N1)-methyltransferase OS=Physcomitrium patens OX=3218 GN=PHYPA...	[more]
F4NUJ6	5.4e-76	35.33	tRNA (guanine(37)-N1)-methyltransferase OS=Batrachochytrium dendrobatidis (strai...	[more]

BLAST of CmaCh12G012350 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HME6	2.4e-276	97.34	tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111465...	[more]
A0A6J1FG25	4.2e-273	96.32	tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1HPS1	1.3e-263	97.23	tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111465...	[more]
A0A6J1FGN5	6.3e-261	96.38	tRNA (guanine(37)-N1)-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1114...	[more]
A0A6J1DUW2	4.4e-238	82.53	tRNA (guanine(37)-N1)-methyltransferase OS=Momordica charantia OX=3673 GN=LOC111...	[more]

BLAST of CmaCh12G012350 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022965706.1	4.9e-276	97.34	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita maxima] >XP_0229...	[more]
XP_022937443.1	8.6e-273	96.32	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita moschata] >XP_02...	[more]
XP_023537242.1	2.6e-269	95.09	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucurbita pepo subsp. pepo...	[more]
XP_023537240.1	4.9e-268	93.03	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X1 [Cucurbita pepo subsp. pepo...	[more]
XP_022965708.1	2.8e-263	97.23	tRNA (guanine(37)-N1)-methyltransferase 2 isoform X2 [Cucurbita maxima]	[more]

BLAST of CmaCh12G012350 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G56120.1	8.0e-184	65.30	S-adenosyl-L-methionine-dependent methyltransferases superfamily protein	[more]
AT4G27340.1	2.4e-55	31.75	Met-10+ like family protein	[more]
AT4G04670.1	9.9e-09	27.81	Met-10+ like family protein / kelch repeat-containing protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.30.300.110		coord: 101..190 e-value: 1.9E-19 score: 71.5
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 192..478 e-value: 4.1E-58 score: 198.0
None	No IPR available	PANTHER	PTHR23245	TRNA METHYLTRANSFERASE	coord: 2..483
None	No IPR available	PANTHER	PTHR23245:SF36	TRNA (GUANINE(37)-N1)-METHYLTRANSFERASE	coord: 2..483
None	No IPR available	CDD	cd02440	AdoMet_MTases	coord: 233..290 e-value: 2.19524E-5 score: 41.6467
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PFAM	PF02475	Met_10	coord: 133..411 e-value: 1.3E-62 score: 211.1
IPR030382	SAM-dependent methyltransferase TRM5/TYW2-type	PROSITE	PS51684	SAM_MT_TRM5_TYW2	coord: 130..480 score: 101.244263
IPR025792	tRNA (guanine(37)-N(1))-methyltransferase, eukaryotic	HAMAP	MF_03152	TRM5	coord: 2..480 score: 25.365166
IPR029063	S-adenosyl-L-methionine-dependent methyltransferase	SUPERFAMILY	53335	S-adenosyl-L-methionine-dependent methyltransferases	coord: 126..480

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G012350.1	CmaCh12G012350.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
cellular_component	GO:0005759	mitochondrial matrix
cellular_component	GO:0005634	nucleus
molecular_function	GO:0052906	tRNA (guanine(37)-N(1))-methyltransferase activity
molecular_function	GO:0009019	tRNA (guanine-N1-)-methyltransferase activity