CmaCh12G011990 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G011990
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description(1->3)-beta-glucan endohydrolase
LocationCma_Chr12: 9400297 .. 9404923 (-)
RNA-Seq ExpressionCmaCh12G011990
SyntenyCmaCh12G011990
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACTTACGCGTTAAGACTTTAGAGAATGAAATTTCGACTAATATTTGTGTATCAAATAACTTCCAATCAAAACCCCTTATCCCTACTGACCCAGAAAACGACCTCCTAAAACCCTTTTCTCATCTCTGTTTCTCTCATCAAAAATCTTCTCTGCCCACTGAGTTTCCACTAGATTCTGAACCAATAGAGAAAAGGGTCTTCGTCCATTTGCGCTAATATTGTTGTTCGGCCCTCGTCTTCTTCTTCCTCGTCCTGTTCAGTGTAGAGATGAGAAATCTGCCCTTTTCGCTTGGGATTTAAGCCAGCTGATCGAATTCCTGTTCCCATATCTCTTGGTGGTTTGATTTTCATTGGCGATTGTTTCTTCTGTTCATGGGTAGGAAGCGCTCTTGGTTCTGTGCTCAATCGGTTCCTGGTTTCTGGGTCGTCTTCTCTGCCATTGGTACTGTAATATTCTCTCTCTGATTTTCGTTTTATGTTTAGTTAATCGATGATTCTGGGATTTTGAGTTGTGGGTTCTGTTTAGCTCTTGAATGATTTTCTTTTTCAGTTCTTCTGTTCTAGATCGATTGGTAGTATTGTATCGTTAAAGATTGCTTCCAGTTGACTTTTATGTTTCTAACTTGCAAGTGTTTTAGATTATTCTTCACTCTGAGACTGCATCAATTTGTTGTTTGATGGGCTATTTTGACTGATTTATAAGTGTATTTTCAATTGAAAAGGGTTATTCTGAGCTAGATTTGAATGCTTCTGTGAGATTTGTTATGTGTGTTAGGAATCACGACTCTCCATAATGGACGATATTGTCCACTTTGAGTATAAGCTCTCGTGGCTTTGCTTTGGGCTTCCCCAAAAGGCCTCGTACCAATGGAAATGTATTCCTTGTAAACTCATTATCATTCCCTAAATTGGCCAATGTGGGACTTCCTCCCAACGGTCCTCAACAATCCTCCCCTCGAACAAAGTACACTATAGAGACTCCCCTAAGGCCTATAGAACCCTTGAACAGTCTCCCCTTAATCAAGTCTCTGACTCCTTTTCTGGAGTCCTCTACCATGTTAGGAATCACAACTCTCTAGAATAGTATGATATTATCAACTTTGAGCATAAGCTCTCATGACTTTGCTTTGGGCTTCCCCAATAGGCCTCATACCAATGGAGATGTATTCCTTACTTATAAACCCATGATTATTTCCTAAATTAGTCAATGTGGGACTCCCTCCCAACAATCCTCAATAATTCTCCCACTCGAACAAAATACCCTATTGAGGGCCTATGGAGCCCTCGAACAACCTCTCCTTAATTGAGGCTCGACTCCTTCTCTAGAGTCCTCGAACAAAATACACCATTTGTTCAACACTTGAGTCAGTTTTGACTACACCTTCGAGGCTCACAACTTCTTTGTTCAACATTTGAGGATTTTATTGGCATGGCTAAGTTGAGGGCATGACTCTGATACCATGTTAGGAATCACGACTCTCCACAATGATATGATATTGTCCACTTTGAGCATAAGCTCTCATGACTTTGCTTTGGGCTTCCCCAAAAGGCCTCATATCAATGGAGATGTATTCCTTACTTTACCCATGATCATTCCTTAAATTAGCCAATGTGGGACTCCCTCCCAACAATCCTCAACAATCCTCTCCCTCGAACAAAGTTCACTATAAAGCCTCCCCTGAGGCCTATGGAGCCCTTAGACAGCCTCCCCTTAATCGAGGCTCGACTCTTTCTCTGGAGTACTCGAACAAAATACACCCTTTGTTTGACACTTGAGTCACTTTTGACTACACCTTTGAGGCTCACAGCTTCTTTGTTCAACATATGAGGATTTTGTTGACATGACTAAGTTAAGGGTATAACTCTGATACCATGTTAGGAATCACAACTCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAGCTTTCATAGCTTTGCTTTGGGCTTCCCATGATCATTCCGTAAATTAGCCAACGTGGCTCAACCATCCTCATATGTTCTCTCATTCTTGAAGTTTTTGTTGAGTCTTGATAGTAAATTGATGTATGCTTACAAGTTAGAGGTTTGAGATAGTTGTAATAGAATGTCTCGTTATATTTCTCCAGCCTCCTTGACAATGGCAGTACAAGCATTTACTGGAACATATGGAATAAATTACGGAAGGATTGCTGATAACATTCCTTCCCCTGATGAAGTTGTTTCGCTTCTCAAGGCAGCGAAAATAAAGAATGTTAGGATTTATGATGCTGATCACAAGGTCCTGAATGCGTTTAGTGGGACGGGGCTTGAATTGGTGGTCGGTCTCCCGAACGGGTTTTTGAAAGAAATGAGTTCGAGTGAGGAACGAGCGATGAACTGGGTGAAGGAAAATGTGCAGGCATTCCTTCCTCAGACTCACATTTGTGGGATTGCCATTGGAAATGAAGTTCTAGGAGGAGGTGACCTTGAGTTGTGGGGAGCACTCTTGGGTGCTACAAAGAATATCTATAAGGCAGTGAAGAAGCTTAACTTAGATGGTGTCATTCAGATCACGACCGCGCATTCTCAGGCGGTTTTTTCGAACTCGTTTCCACCTTCTTCGTGTATCTTTAGAGAAAATGTTGTTCAGTACATGAAGCCTTTGTTGGAGTTTTTTTCAGAGATCGATTCGCCTTTCTGTTTAAATGCATACCCTTTCCTAGCTTATATGAGTGATCCAGAGAATATTGATATCAACTATGCACTTTTCGAATCAACCGAGGGGATTTTTGATCCTAAAACCAATCTACGATATGACAACATGCTCGATGCTCAGATCGACGCTGCATATGCAGCATTGGCTGATGCTGGATATCATAAAATGGAAGTTATAGTTACCGAGACCGGTTGGGCTTCCCGTGGGGACGAAAATGAAGCTGCAGCAACAGCAAATAATGCTAAGATCTTTAATTACAACCTCAGAAAGAGGCTTGCTAAGAAGAAAGGAACTCCCTTCAGGCCAAAGAATGTATTGAAGGCCTATATTTTTGCAACTTTTAATGAGAATTTGAAACCAGGGCCAACTTCTGAGAGAAATTTTGGACTTTTCAAAGCTGATGGGACCATATCATATGACATTGGATTTCATGGGCTTCAATCTTCATCTTCAAGTTCATCTTATTTATCTCTCAAGGTATGTAATTCACGTGCTATTGTCTTCTAATCCCGTTAAGAATCACGACCTCCACAATGGTATGATATTATCCACTTTGAGCATAAGCTCTCGTGGTTTTGCTTTTGGCTTCCTGGTATTCCTTATAAACTCATGATCATTCCCTAAATTAGCCAATGTGGGATTCCCTCCCAACAATCCTCCCCTCGAACAAAGTACACCATAGAGCCTCCCCTGAGGCCTATGGAGCCCTCGAACAGTCTCCCCTTAATCGAGGCTCGACTCCTTCTCTAGAGTCCTTGAACAAAGTTACACCCTTTGTTCGACACTTGAGTCACTTTTGACTACATCTTTGAGGGTCCCAACTTCTTTGTTCAAAATTTGAGGATTCTATTAACATGGCTAAGTTAAGGGTGTGACTCTAATACCATTTTAAGAATCACGACCTCCACAATGGTATGATATTGTCCACTTTGAACGTAAGCTCTCGTGGCTTTGCTTTTGGATTCCCCGAAAGGCCTCATACCAATGGAGATATATTGCTTATTTATAAACTCAGGATCATTCCCTACATTAGCCAATGTGGGACTCCTTCCCAACAATCCTCCCTTCGAACAAAGTACACTATAGAGCCTCCCCTGAGGCATATGGAGCCCTCGAACAGCCTCCCCTTAATTGAGGCTCAACTCCTTCGCTAGAGTCCTCGAACAAAGGACACTCTTTGTTCGACACTTGAGTCACTTTTAACTACACCTTCGAGGGTCTCGACTTCTTTGTTCGACATCTGAGGATTCTATTGACATGGCTAAGTTAAGGGCTTGACTCTGATATCATGTTAAGAATCACGACCTCCACAATGGTATGATATTGTCCACTTTGAGCATAAACTCTTATGGCTTTGCTTTTAGCTTCCCCAAAATGCCTCATACCAATGGAGAGTATTCCTTACTTAAAACCCATGATTAATGTGGGACTCCCTCCCAACAATCCTCAACAAATCCGACTTTCTTTCTTGGTTTGTACTAAATAACCGTTCTTAACGATATCCAACACATTCTGCTTGCTTATGTGAACAGAATCACCGAGCTCGAGGACTTGCAGGAAGCCTTATTTTCTCATTAACAGTCTCGGTCATGACGCGGCTTCTATTGTTGAGATGAAAATAGAGTATCTTAAGTTAGGAAGGTGAAGGTAAAGGTGCAGCAGCGGGTGGAAAACATTGAATCTTGTGCTGGTATATGTCATGATACAGTTGCTGTTTATTCTTTTGCTATAAAATGTATCCCAATTTCCCTCCAGGTTGAGTTTGAGATGAAGCTATTGAATCATAAATGGAAGTGCCAAAATATGCTTATGAGTTCCTGATGTCCCAGTTTGAGTATATCTCTGATATAATCTTGGTTGAAACTGTCTTGGGTTGCTTTGTGTTTAGTGAGATTAACCAATTTGTAGCCTTGAGTAAGCAAGTTTATGTATCGAACACAAGCTCGACC

mRNA sequence

ACTTACGCGTTAAGACTTTAGAGAATGAAATTTCGACTAATATTTGTGTATCAAATAACTTCCAATCAAAACCCCTTATCCCTACTGACCCAGAAAACGACCTCCTAAAACCCTTTTCTCATCTCTGTTTCTCTCATCAAAAATCTTCTCTGCCCACTGAGTTTCCACTAGATTCTGAACCAATAGAGAAAAGGGTCTTCGTCCATTTGCGCTAATATTGTTGTTCGGCCCTCGTCTTCTTCTTCCTCGTCCTGTTCAGTGTAGAGATGAGAAATCTGCCCTTTTCGCTTGGGATTTAAGCCAGCTGATCGAATTCCTGTTCCCATATCTCTTGGTGGTTTGATTTTCATTGGCGATTGTTTCTTCTGTTCATGGGTAGGAAGCGCTCTTGGTTCTGTGCTCAATCGGTTCCTGGTTTCTGGGTCGTCTTCTCTGCCATTGCCTCCTTGACAATGGCAGTACAAGCATTTACTGGAACATATGGAATAAATTACGGAAGGATTGCTGATAACATTCCTTCCCCTGATGAAGTTGTTTCGCTTCTCAAGGCAGCGAAAATAAAGAATGTTAGGATTTATGATGCTGATCACAAGGTCCTGAATGCGTTTAGTGGGACGGGGCTTGAATTGGTGGTCGGTCTCCCGAACGGGTTTTTGAAAGAAATGAGTTCGAGTGAGGAACGAGCGATGAACTGGGTGAAGGAAAATGTGCAGGCATTCCTTCCTCAGACTCACATTTGTGGGATTGCCATTGGAAATGAAGTTCTAGGAGGAGGTGACCTTGAGTTGTGGGGAGCACTCTTGGGTGCTACAAAGAATATCTATAAGGCAGTGAAGAAGCTTAACTTAGATGGTGTCATTCAGATCACGACCGCGCATTCTCAGGCGGTTTTTTCGAACTCGTTTCCACCTTCTTCGTGTATCTTTAGAGAAAATGTTGTTCAGTACATGAAGCCTTTGTTGGAGTTTTTTTCAGAGATCGATTCGCCTTTCTGTTTAAATGCATACCCTTTCCTAGCTTATATGAGTGATCCAGAGAATATTGATATCAACTATGCACTTTTCGAATCAACCGAGGGGATTTTTGATCCTAAAACCAATCTACGATATGACAACATGCTCGATGCTCAGATCGACGCTGCATATGCAGCATTGGCTGATGCTGGATATCATAAAATGGAAGTTATAGTTACCGAGACCGGTTGGGCTTCCCGTGGGGACGAAAATGAAGCTGCAGCAACAGCAAATAATGCTAAGATCTTTAATTACAACCTCAGAAAGAGGCTTGCTAAGAAGAAAGGAACTCCCTTCAGGCCAAAGAATGTATTGAAGGCCTATATTTTTGCAACTTTTAATGAGAATTTGAAACCAGGGCCAACTTCTGAGAGAAATTTTGGACTTTTCAAAGCTGATGGGACCATATCATATGACATTGGATTTCATGGGCTTCAATCTTCATCTTCAAGTTCATCTTATTTATCTCTCAAGAATCACCGAGCTCGAGGACTTGCAGGAAGCCTTATTTTCTCATTAACAGTCTCGGTCATGACGCGGCTTCTATTGTTGAGATGAAAATAGAGTATCTTAAGTTAGGAAGGTGAAGGTAAAGGTGCAGCAGCGGGTGGAAAACATTGAATCTTGTGCTGGTATATGTCATGATACAGTTGCTGTTTATTCTTTTGCTATAAAATGTATCCCAATTTCCCTCCAGGTTGAGTTTGAGATGAAGCTATTGAATCATAAATGGAAGTGCCAAAATATGCTTATGAGTTCCTGATGTCCCAGTTTGAGTATATCTCTGATATAATCTTGGTTGAAACTGTCTTGGGTTGCTTTGTGTTTAGTGAGATTAACCAATTTGTAGCCTTGAGTAAGCAAGTTTATGTATCGAACACAAGCTCGACC

Coding sequence (CDS)

ATGGGTAGGAAGCGCTCTTGGTTCTGTGCTCAATCGGTTCCTGGTTTCTGGGTCGTCTTCTCTGCCATTGCCTCCTTGACAATGGCAGTACAAGCATTTACTGGAACATATGGAATAAATTACGGAAGGATTGCTGATAACATTCCTTCCCCTGATGAAGTTGTTTCGCTTCTCAAGGCAGCGAAAATAAAGAATGTTAGGATTTATGATGCTGATCACAAGGTCCTGAATGCGTTTAGTGGGACGGGGCTTGAATTGGTGGTCGGTCTCCCGAACGGGTTTTTGAAAGAAATGAGTTCGAGTGAGGAACGAGCGATGAACTGGGTGAAGGAAAATGTGCAGGCATTCCTTCCTCAGACTCACATTTGTGGGATTGCCATTGGAAATGAAGTTCTAGGAGGAGGTGACCTTGAGTTGTGGGGAGCACTCTTGGGTGCTACAAAGAATATCTATAAGGCAGTGAAGAAGCTTAACTTAGATGGTGTCATTCAGATCACGACCGCGCATTCTCAGGCGGTTTTTTCGAACTCGTTTCCACCTTCTTCGTGTATCTTTAGAGAAAATGTTGTTCAGTACATGAAGCCTTTGTTGGAGTTTTTTTCAGAGATCGATTCGCCTTTCTGTTTAAATGCATACCCTTTCCTAGCTTATATGAGTGATCCAGAGAATATTGATATCAACTATGCACTTTTCGAATCAACCGAGGGGATTTTTGATCCTAAAACCAATCTACGATATGACAACATGCTCGATGCTCAGATCGACGCTGCATATGCAGCATTGGCTGATGCTGGATATCATAAAATGGAAGTTATAGTTACCGAGACCGGTTGGGCTTCCCGTGGGGACGAAAATGAAGCTGCAGCAACAGCAAATAATGCTAAGATCTTTAATTACAACCTCAGAAAGAGGCTTGCTAAGAAGAAAGGAACTCCCTTCAGGCCAAAGAATGTATTGAAGGCCTATATTTTTGCAACTTTTAATGAGAATTTGAAACCAGGGCCAACTTCTGAGAGAAATTTTGGACTTTTCAAAGCTGATGGGACCATATCATATGACATTGGATTTCATGGGCTTCAATCTTCATCTTCAAGTTCATCTTATTTATCTCTCAAGAATCACCGAGCTCGAGGACTTGCAGGAAGCCTTATTTTCTCATTAACAGTCTCGGTCATGACGCGGCTTCTATTGTTGAGATGA

Protein sequence

MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQTHICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQSSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR
Homology
BLAST of CmaCh12G011990 vs. ExPASy Swiss-Prot
Match: Q8L868 (Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860 PE=2 SV=1)

HSP 1 Score: 328.2 bits (840), Expect = 1.3e-88
Identity = 160/337 (47.48%), Postives = 226/337 (67.06%), Query Frame = 0

Query: 23  IASLTMAVQAFTGT-YGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLNAFSG 82
           + SLT+ +   T T  G+NYG+I DN+PSP +V+ L+K+     V++YDA+ ++L AFS 
Sbjct: 14  LISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSN 73

Query: 83  TGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQTHICGIAIGNEVLGGGDLELWG 142
           TG+E ++GL N +L +M     +A+ W+K+NV  FLP T+I  I IGNE+L   D  L  
Sbjct: 74  TGIEFIIGLGNEYLSKM-KDPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTT 133

Query: 143 ALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFS 202
            LL A + ++ A+    L   I +TTAHS ++  +SFPPS+  F+ +++  + P+LEF  
Sbjct: 134 NLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHR 193

Query: 203 EIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQIDAAYAAL 262
           + DSPF +NAYPF AY  +P+ + +++ LF+  +GI DP T   YDNML AQIDA Y+AL
Sbjct: 194 KTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSAL 253

Query: 263 ADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYNLRKRL--AKKKGTPFRPKNVL 322
           A AG+  + V ++ETGW S+GD++E  AT  NAK +N NL K +   KK  TP +P N L
Sbjct: 254 AAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDL 313

Query: 323 KAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGF 357
             Y+FA FNENLKPGPTSERN+GLFK DGT +Y +GF
Sbjct: 314 SIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGF 349

BLAST of CmaCh12G011990 vs. ExPASy Swiss-Prot
Match: Q9ZQG9 (Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500 PE=2 SV=2)

HSP 1 Score: 319.3 bits (817), Expect = 6.1e-86
Identity = 147/321 (45.79%), Postives = 218/321 (67.91%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKE 97
           GINYG+IA+N+PSP  V  LL++  I  V++YDAD  VL +FS + ++ ++GL N +L+ 
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 98  MSSSEERAMNWVKENVQAFLPQTHICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKL 157
           MS+   +A +W+++ ++  + +T I  I +GNE+    D  L  +LL A K++Y A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 158 NLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAY 217
            L+  + +T+AHS  + S S+PPSS  F+E  +QY++PLL+F S+I+SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 218 MSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETG 277
              P+ + + Y LF+  +G+ DP TNL YDNML AQ+DA Y+A+   G+  +EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 278 WASRGDENEAAATANNAKIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           W S+GDENE  A+  NA ++N NL K + ++KGTP +    +  Y+FA FNENLKPGP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 338 ERNFGLFKADGTISYDIGFHG 359
           ERN+GLF  DG   Y++G  G
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQG 350

BLAST of CmaCh12G011990 vs. ExPASy Swiss-Prot
Match: Q9FHX5 (Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100 PE=2 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 2.7e-81
Identity = 156/351 (44.44%), Postives = 220/351 (62.68%), Query Frame = 0

Query: 19  VFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLNA 78
           +FS       ++     + GINYG++A+N+P P  V+ LLK+     V++YDAD + L A
Sbjct: 9   LFSLFCLALFSLPLIVSSIGINYGQVANNLPPPKNVIPLLKSVGATKVKLYDADPQALRA 68

Query: 79  FSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQTHICGIAIGNEVLGGGDLE 138
           F+G+G EL V L N +L +M S   +A  WVKENVQA+LP T I  I +GNEVL      
Sbjct: 69  FAGSGFELTVALGNEYLAQM-SDPIKAQGWVKENVQAYLPNTKIVAIVVGNEVLTSNQSA 128

Query: 139 LWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLE 198
           L  AL  A ++I+ A+    L+  I +TTAHS A+   S+PPS+  FR +++  + P+L+
Sbjct: 129 LTAALFPAMQSIHGALVDCGLNKQIFVTTAHSLAILDVSYPPSATSFRRDLLGSLTPILD 188

Query: 199 FFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQIDAAY 258
           F  +  SP  +NAYPF AY  +P+++ +++ LF+  +G  DP +N  YDNML AQ+DA Y
Sbjct: 189 FHVKTGSPILINAYPFFAYEENPKHVSLDFVLFQPNQGFTDPGSNFHYDNMLFAQVDAVY 248

Query: 259 AALADAG--YHKMEVIVTETGWASRGDENEAAATANNAKIFNYNLRK-RLAKKKGTPFRP 318
            AL   G  Y K+ ++V+ETGW S GD  E  AT +NA+ +N NL K  ++KK  TP RP
Sbjct: 249 HALDAVGISYKKVPIVVSETGWPSNGDPQEVGATCDNARKYNGNLIKMMMSKKMRTPIRP 308

Query: 319 KNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQSSSSSS 367
           +  L  ++FA FNEN+KPGPTSERN+GLF  DGT  Y +G     + SS S
Sbjct: 309 ECDLTIFVFALFNENMKPGPTSERNYGLFNPDGTPVYSLGIKTSSTHSSGS 358

BLAST of CmaCh12G011990 vs. ExPASy Swiss-Prot
Match: Q9M069 (Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 PE=1 SV=2)

HSP 1 Score: 300.4 bits (768), Expect = 2.9e-80
Identity = 147/330 (44.55%), Postives = 206/330 (62.42%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKE 97
           G+NYG++ADN+P P E V LL++  I+ VR+Y AD  ++ A +GTG+ +V+G  NG +  
Sbjct: 27  GVNYGQVADNLPPPSETVKLLQSTSIQKVRLYGADPAIIKALAGTGVGIVIGAANGDVPS 86

Query: 98  MSSSEERAMNWVKENVQAFLPQTHICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKL 157
           ++S    A  W+  NV  F P + I  I +GNE+L   D  L   LL A +N+ KA++ +
Sbjct: 87  LASDPNAATQWINSNVLPFYPASKIMLITVGNEILMSNDPNLVNQLLPAMQNVQKALEAV 146

Query: 158 NLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAY 217
           +L G I+++T +S  V  +S PPSS  F       +K +L+F S+  SPF +N YPF AY
Sbjct: 147 SLGGKIKVSTVNSMTVLGSSDPPSSGSFAAGYQTGLKGILQFLSDTGSPFAINPYPFFAY 206

Query: 218 MSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETG 277
            SDP    + + LFE   G  D KT ++Y NM DAQ+DA ++AL   G+ K+E++V ETG
Sbjct: 207 QSDPRPETLAFCLFEPNAGRVDSKTGIKYTNMFDAQVDAVHSALKSMGFEKVEIVVAETG 266

Query: 278 WASRGDENEAAATANNAKIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           WASRGD NE  A+ +NAK +N NL   L    GTP  P   +  YIFA ++ENLKPGP+S
Sbjct: 267 WASRGDANEVGASVDNAKAYNGNLIAHLRSMVGTPLMPGKPVDTYIFALYDENLKPGPSS 326

Query: 338 ERNFGLFKADGTISYDIGFHGLQSSSSSSS 368
           ER FGLFK D ++ YD+G     SSS + S
Sbjct: 327 ERAFGLFKTDLSMVYDVGLAKSSSSSQTPS 356

BLAST of CmaCh12G011990 vs. ExPASy Swiss-Prot
Match: Q8VYE5 (Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360 PE=2 SV=1)

HSP 1 Score: 286.2 bits (731), Expect = 5.7e-76
Identity = 148/343 (43.15%), Postives = 207/343 (60.35%), Query Frame = 0

Query: 16  FWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKV 75
           FW+  S +A L   +       GI YGR ADN+PSP+ V  L++   IK VRIYDA+  V
Sbjct: 9   FWIFVSILAFLNFGM---ASKIGICYGRNADNLPSPNRVSELIQHLNIKFVRIYDANIDV 68

Query: 76  LNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQTHICGIAIGNEVLGGG 135
           L AF+ TG+EL++G+PN  L   +  +     W+  N+  + P T I  I++G EV    
Sbjct: 69  LKAFANTGIELMIGVPNADLLAFAQFQSNVDTWLSNNILPYYPSTKITSISVGLEVTEAP 128

Query: 136 DLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMKP 195
           D    G +L A +NI+ A+KK  LD  I+I+++HS A+ S SFPPSS  F +    ++KP
Sbjct: 129 D-NATGLVLPAMRNIHTALKKSGLDKKIKISSSHSLAILSRSFPPSSASFSKKHSAFLKP 188

Query: 196 LLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQID 255
           +LEF  E +SPF ++ YP+ AY    E + + YALFES+  + DP T L Y NM DAQ+D
Sbjct: 189 MLEFLVENESPFMIDLYPYYAYRDSTEKVPLEYALFESSSQVVDPATGLLYSNMFDAQLD 248

Query: 256 AAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYNLRKRLAKKKGTPFRP 315
           A Y AL    +  ++V+VTE+GW S+G   E AAT  NA  +N NL + +    GTP +P
Sbjct: 249 AIYFALTAMSFKTVKVMVTESGWPSKGSPKETAATPENALAYNTNLIRHVIGDPGTPAKP 308

Query: 316 KNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHG 359
              +  Y+F+ FNEN KPG  SERN+G+F A+GT  Y + F G
Sbjct: 309 GEEIDVYLFSLFNENRKPGIESERNWGMFYANGTNVYALDFTG 347

BLAST of CmaCh12G011990 vs. ExPASy TrEMBL
Match: A0A6J1HML7 ((1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465570 PE=3 SV=1)

HSP 1 Score: 794.3 bits (2050), Expect = 2.4e-226
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300
           KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 400
           SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR
Sbjct: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 399

BLAST of CmaCh12G011990 vs. ExPASy TrEMBL
Match: A0A6J1FIR4 ((1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111444294 PE=3 SV=1)

HSP 1 Score: 786.9 bits (2031), Expect = 3.8e-224
Identity = 394/399 (98.75%), Postives = 397/399 (99.50%), Query Frame = 0

Query: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MGRKR WFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRFWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLNAFSGTGLELV+GLPNGFLKEMSSSEERAMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVIGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGV+QITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEI SPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300
           KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 400
           SSSSSSSYLSLKNHRARGLAGSLIFSLTVSV+TRLLLLR
Sbjct: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVVTRLLLLR 399

BLAST of CmaCh12G011990 vs. ExPASy TrEMBL
Match: A0A1S3C5G6 ((1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497110 PE=3 SV=1)

HSP 1 Score: 725.7 bits (1872), Expect = 1.0e-205
Identity = 362/399 (90.73%), Postives = 377/399 (94.49%), Query Frame = 0

Query: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK  WFC QS+PGFWVVFSAIASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSIPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AFSGTGLELVVGLPNGFL+EMS+SEE AMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGA KNIYKAVK LNLDGV+QITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEI SPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300
           KT L YDNMLDAQIDAAYAALADAGY KMEVIVTETGWASRGDENEAAATA+NA+IFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 400
           SSSS SSYLSLK+ RARGL+GS + SLT+S +T LLLLR
Sbjct: 361 SSSSDSSYLSLKDFRARGLSGSHMISLTISAVTLLLLLR 399

BLAST of CmaCh12G011990 vs. ExPASy TrEMBL
Match: A0A0A0LF78 ((1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G006850 PE=3 SV=1)

HSP 1 Score: 717.6 bits (1851), Expect = 2.8e-203
Identity = 359/399 (89.97%), Postives = 373/399 (93.48%), Query Frame = 0

Query: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK  WFC QS+ GFWVVFSAIASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSLSGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AFSGTGLELVVGLPNGFL+EMS+SEE AMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWG LLGA KNIYKAVK LNLDGV+QITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGTLLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEI SPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300
           KT L YDNMLDAQIDAAYAALADAGY KMEVIVTETGWASRGDENEAAATA+NA+IFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 400
            SSS SSYLSLK+ RARGL GS + SLT++ MT LLLLR
Sbjct: 361 YSSSDSSYLSLKDFRARGLLGSHMISLTITAMTLLLLLR 399

BLAST of CmaCh12G011990 vs. ExPASy TrEMBL
Match: A0A6J1CPQ3 ((1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111013168 PE=3 SV=1)

HSP 1 Score: 711.4 bits (1835), Expect = 2.0e-201
Identity = 354/392 (90.31%), Postives = 371/392 (94.64%), Query Frame = 0

Query: 8   FCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVR 67
           FC QS+P FWVVFSAIASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVR
Sbjct: 10  FCVQSLPSFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVR 69

Query: 68  IYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQTHICGIAI 127
           IYDADH+VL AFSGTGLELVVGLPNGFLKEMS+SE+RAMNWVKENVQAFLPQTHICGIAI
Sbjct: 70  IYDADHRVLKAFSGTGLELVVGLPNGFLKEMSASEDRAMNWVKENVQAFLPQTHICGIAI 129

Query: 128 GNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPPSSCIFRE 187
           GNEVLGGGDLELWGALLGA KNIYKAVKKLNLDG++QITTAHSQAVFSNSFPPSSCIFRE
Sbjct: 130 GNEVLGGGDLELWGALLGAAKNIYKAVKKLNLDGIVQITTAHSQAVFSNSFPPSSCIFRE 189

Query: 188 NVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTNLRYD 247
           NVVQYMKPLL+FFSEI SPFCLNAYPFLAYMSDPENIDINYALFEST+GI DPKT L YD
Sbjct: 190 NVVQYMKPLLDFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTQGISDPKTGLHYD 249

Query: 248 NMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYNLRKRLAK 307
           NMLDAQIDAAYAAL DAGY KMEVIVTETGWAS GDENEAAATANNA+IFNYNLRKRLAK
Sbjct: 250 NMLDAQIDAAYAALVDAGYQKMEVIVTETGWASHGDENEAAATANNARIFNYNLRKRLAK 309

Query: 308 KKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQSSSSSSS 367
           KKGTPFRPKNVLKAYIFATFNEN+KPGPTSERNFGLFKADGTISYDIGFHGL+SSS+ SS
Sbjct: 310 KKGTPFRPKNVLKAYIFATFNENMKPGPTSERNFGLFKADGTISYDIGFHGLKSSSADSS 369

Query: 368 YLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 400
           YLSL++ +ARG   + IFSLT+  MT LLLLR
Sbjct: 370 YLSLQDLQARGFLVNRIFSLTIPAMTLLLLLR 401

BLAST of CmaCh12G011990 vs. NCBI nr
Match: XP_022965781.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita maxima])

HSP 1 Score: 794.3 bits (2050), Expect = 4.9e-226
Identity = 399/399 (100.00%), Postives = 399/399 (100.00%), Query Frame = 0

Query: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300
           KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 400
           SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR
Sbjct: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 399

BLAST of CmaCh12G011990 vs. NCBI nr
Match: XP_022938105.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita moschata])

HSP 1 Score: 786.9 bits (2031), Expect = 7.9e-224
Identity = 394/399 (98.75%), Postives = 397/399 (99.50%), Query Frame = 0

Query: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MGRKR WFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRFWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLNAFSGTGLELV+GLPNGFLKEMSSSEERAMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVIGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGV+QITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEI SPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300
           KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 400
           SSSSSSSYLSLKNHRARGLAGSLIFSLTVSV+TRLLLLR
Sbjct: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVVTRLLLLR 399

BLAST of CmaCh12G011990 vs. NCBI nr
Match: KAG6586386.1 (Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 785.4 bits (2027), Expect = 2.3e-223
Identity = 393/399 (98.50%), Postives = 397/399 (99.50%), Query Frame = 0

Query: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MGRKR WFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRFWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLNAFSGTGLELV+GLPNGFLKEMSSSEERAMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVIGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGV+QITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYM+PLLEFFSEI SPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMEPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300
           KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 400
           SSSSSSSYLSLKNHRARGLAGSLIFSLTVSV+TRLLLLR
Sbjct: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVVTRLLLLR 399

BLAST of CmaCh12G011990 vs. NCBI nr
Match: XP_023537789.1 (glucan endo-1,3-beta-glucosidase 11-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 781.9 bits (2018), Expect = 2.5e-222
Identity = 391/399 (97.99%), Postives = 395/399 (99.00%), Query Frame = 0

Query: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVLNAFSGTGLELV+GLPNGFLKEMSSSEERAMNWVKENVQAFLPQT
Sbjct: 61  AKIKNVRIYDADHKVLNAFSGTGLELVIGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGV+QITTAHSQAVFSNSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVVQITTAHSQAVFSNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEFFSEI SPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFFSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240

Query: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300
           KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN
Sbjct: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360
           LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ
Sbjct: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360

Query: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 400
           SSSS SSYLSL+NHRARGLAGSLIFSLT SV+T LLLLR
Sbjct: 361 SSSSGSSYLSLENHRARGLAGSLIFSLTASVVTPLLLLR 399

BLAST of CmaCh12G011990 vs. NCBI nr
Match: XP_008457422.1 (PREDICTED: glucan endo-1,3-beta-glucosidase 11 [Cucumis melo])

HSP 1 Score: 725.7 bits (1872), Expect = 2.2e-205
Identity = 362/399 (90.73%), Postives = 377/399 (94.49%), Query Frame = 0

Query: 1   MGRKRSWFCAQSVPGFWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60
           M RK  WFC QS+PGFWVVFSAIASLTM VQAFTGTYGINYGRIADNIPSPDEVVSLLKA
Sbjct: 1   MARKCFWFCFQSIPGFWVVFSAIASLTMTVQAFTGTYGINYGRIADNIPSPDEVVSLLKA 60

Query: 61  AKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQT 120
           AKIKNVRIYDADHKVL AFSGTGLELVVGLPNGFL+EMS+SEE AMNWVKENVQAFLP+T
Sbjct: 61  AKIKNVRIYDADHKVLKAFSGTGLELVVGLPNGFLREMSASEEHAMNWVKENVQAFLPET 120

Query: 121 HICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPP 180
           HICGIAIGNEVLGGGDLELWGALLGA KNIYKAVK LNLDGV+QITTAHSQAVF NSFPP
Sbjct: 121 HICGIAIGNEVLGGGDLELWGALLGAAKNIYKAVKNLNLDGVVQITTAHSQAVFWNSFPP 180

Query: 181 SSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDP 240
           SSCIFRENVVQYMKPLLEF SEI SPFCLNAYPFLAYMSDPENIDINYALFEST+GIFDP
Sbjct: 181 SSCIFRENVVQYMKPLLEFLSEIGSPFCLNAYPFLAYMSDPENIDINYALFESTKGIFDP 240

Query: 241 KTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYN 300
           KT L YDNMLDAQIDAAYAALADAGY KMEVIVTETGWASRGDENEAAATA+NA+IFNYN
Sbjct: 241 KTGLHYDNMLDAQIDAAYAALADAGYQKMEVIVTETGWASRGDENEAAATASNARIFNYN 300

Query: 301 LRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQ 360
           LRKRLAKKKGTPFRPKN LKAYIFATFNENLKPGPTSERNFGLFKADG+ISYDIGFHGL+
Sbjct: 301 LRKRLAKKKGTPFRPKNALKAYIFATFNENLKPGPTSERNFGLFKADGSISYDIGFHGLK 360

Query: 361 SSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLLLR 400
           SSSS SSYLSLK+ RARGL+GS + SLT+S +T LLLLR
Sbjct: 361 SSSSDSSYLSLKDFRARGLSGSHMISLTISAVTLLLLLR 399

BLAST of CmaCh12G011990 vs. TAIR 10
Match: AT2G26600.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 530.0 bits (1364), Expect = 1.6e-150
Identity = 268/384 (69.79%), Postives = 312/384 (81.25%), Query Frame = 0

Query: 16  FWVVFSAIASLTMAVQ-AFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHK 75
           F+  F  + SL   V  AF GTYGINYGRIADNIPSP++VV LLK AKI+NVRIYD DH 
Sbjct: 12  FFFFFFNLTSLEYQVDGAFVGTYGINYGRIADNIPSPEKVVLLLKQAKIRNVRIYDVDHT 71

Query: 76  VLNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQTHICGIAIGNEVLGG 135
           VL AFSGTGL+LVVGLPNGFLKEMSS+ + A +WVKEN+Q+FLP+T I GIAIGNEVLGG
Sbjct: 72  VLEAFSGTGLDLVVGLPNGFLKEMSSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGG 131

Query: 136 GDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMK 195
           GD EL GALLGA KN+Y A+KK+NL+  +QITTAHSQAVFS+S+PPSSC+F+ENVVQ+MK
Sbjct: 132 GDSELAGALLGAAKNVYNALKKMNLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMK 191

Query: 196 PLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQI 255
           PLLEFF +I SPFCLNAYPFLAY  +P+ IDINYALF+ TEGI+DPKT+L YDNMLDAQI
Sbjct: 192 PLLEFFQQIGSPFCLNAYPFLAYTYNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQI 251

Query: 256 DAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYNLRKRLAKKKGTPFR 315
           DAAY AL DAG+ KMEV++TETGWAS+GD +E AAT  NA+ +NYNLRKRLAKKKGTP R
Sbjct: 252 DAAYMALQDAGFKKMEVMITETGWASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLR 311

Query: 316 PKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQSSSSSSSYLSLKNH 375
           PK VLKAYIFA FNEN KPG +SE +FGLFK DGTISYDIGF+ L+S S  S   S K+ 
Sbjct: 312 PKTVLKAYIFALFNENSKPGKSSETHFGLFKPDGTISYDIGFNNLKSDSPKSLISSSKSA 371

Query: 376 RARGLAGSLIFSLTVSVMTRLLLL 399
           R          +L +SV   LL++
Sbjct: 372 R-------YYVALVISVSAFLLMI 388

BLAST of CmaCh12G011990 vs. TAIR 10
Match: AT3G15800.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 468.4 bits (1204), Expect = 5.8e-132
Identity = 225/351 (64.10%), Postives = 281/351 (80.06%), Query Frame = 0

Query: 16  FWVVFSAIASLTMAVQAFTGTYGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKV 75
           F +VFS ++S T    AF GTYG+NYGRIADN+PSPD V +LLK+AKI+N RIYDADH V
Sbjct: 24  FLLVFSILSSQTAV--AFIGTYGVNYGRIADNLPSPDAVATLLKSAKIRNTRIYDADHSV 83

Query: 76  LNAFSGTGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFL-PQTHICGIAIGNEVLGG 135
           L AF GTG+E++VGL N FLK++S  E+RAMNW+KENV+ F+   T I GIA+GNE+LGG
Sbjct: 84  LTAFRGTGIEIIVGLGNEFLKDISVGEDRAMNWIKENVEPFIRGGTKISGIAVGNEILGG 143

Query: 136 GDLELWGALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMK 195
            D+ LW ALL A KN+Y A+++L L  V+++++ HS+AVF+NS+PPSSC FR++V  +MK
Sbjct: 144 TDIGLWEALLPAAKNVYSALRRLGLHNVVEVSSPHSEAVFANSYPPSSCTFRDDVAPFMK 203

Query: 196 PLLEFFSEIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQI 255
           PLL FF +I SPF +NAYPFLAY SDP  IDINYALFE  +GI DPKT L YDNM DA +
Sbjct: 204 PLLAFFWQIQSPFYINAYPFLAYKSDPITIDINYALFEHNKGILDPKTKLHYDNMFDAMV 263

Query: 256 DAAYAALADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYNLRKRLAKKKGTPFR 315
           DA+YAAL  AGY K+ VIV+ETGWAS+GD +E  A+  NA+ +N NLRKRL K+KGTP+R
Sbjct: 264 DASYAALEKAGYTKVPVIVSETGWASKGDADEPGASVKNARTYNRNLRKRLQKRKGTPYR 323

Query: 316 PKNVLKAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGFHGLQSSSSS 366
           P  V++AY+FA FNEN KPGPTSERNFGLFK DGTI+YDIG  GL+SSS++
Sbjct: 324 PDMVVRAYVFALFNENSKPGPTSERNFGLFKPDGTIAYDIGLTGLKSSSAT 372

BLAST of CmaCh12G011990 vs. TAIR 10
Match: AT2G26600.2 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 414.8 bits (1065), Expect = 7.6e-116
Identity = 207/301 (68.77%), Postives = 245/301 (81.40%), Query Frame = 0

Query: 98  MSSSEERAMNWVKENVQAFLPQTHICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKL 157
           MSS+ + A +WVKEN+Q+FLP+T I GIAIGNEVLGGGD EL GALLGA KN+Y A+KK+
Sbjct: 1   MSSNADHAFSWVKENIQSFLPKTRIRGIAIGNEVLGGGDSELAGALLGAAKNVYNALKKM 60

Query: 158 NLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAY 217
           NL+  +QITTAHSQAVFS+S+PPSSC+F+ENVVQ+MKPLLEFF +I SPFCLNAYPFLAY
Sbjct: 61  NLEDTVQITTAHSQAVFSDSYPPSSCVFKENVVQFMKPLLEFFQQIGSPFCLNAYPFLAY 120

Query: 218 MSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETG 277
             +P+ IDINYALF+ TEGI+DPKT+L YDNMLDAQIDAAY AL DAG+ KMEV++TETG
Sbjct: 121 TYNPKEIDINYALFKPTEGIYDPKTDLHYDNMLDAQIDAAYMALQDAGFKKMEVMITETG 180

Query: 278 WASRGDENEAAATANNAKIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           WAS+GD +E AAT  NA+ +NYNLRKRLAKKKGTP RPK VLKAYIFA FNEN KPG +S
Sbjct: 181 WASKGDSDEPAATPENARTYNYNLRKRLAKKKGTPLRPKTVLKAYIFALFNENSKPGKSS 240

Query: 338 ERNFGLFKADGTISYDIGFHGLQSSSSSSSYLSLKNHRARGLAGSLIFSLTVSVMTRLLL 397
           E +FGLFK DGTISYDIGF+ L+S S  S   S K+ R          +L +SV   LL+
Sbjct: 241 ETHFGLFKPDGTISYDIGFNNLKSDSPKSLISSSKSAR-------YYVALVISVSAFLLM 294

Query: 398 L 399
           +
Sbjct: 301 I 294

BLAST of CmaCh12G011990 vs. TAIR 10
Match: AT1G32860.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 328.2 bits (840), Expect = 9.4e-90
Identity = 160/337 (47.48%), Postives = 226/337 (67.06%), Query Frame = 0

Query: 23  IASLTMAVQAFTGT-YGINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLNAFSG 82
           + SLT+ +   T T  G+NYG+I DN+PSP +V+ L+K+     V++YDA+ ++L AFS 
Sbjct: 14  LISLTLIILPTTTTSIGVNYGQIGDNLPSPTDVIPLIKSIGATKVKLYDANPQILKAFSN 73

Query: 83  TGLELVVGLPNGFLKEMSSSEERAMNWVKENVQAFLPQTHICGIAIGNEVLGGGDLELWG 142
           TG+E ++GL N +L +M     +A+ W+K+NV  FLP T+I  I IGNE+L   D  L  
Sbjct: 74  TGIEFIIGLGNEYLSKM-KDPSKALTWIKQNVTPFLPATNITCITIGNEILALNDSSLTT 133

Query: 143 ALLGATKNIYKAVKKLNLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFS 202
            LL A + ++ A+    L   I +TTAHS ++  +SFPPS+  F+ +++  + P+LEF  
Sbjct: 134 NLLPAMQGVHSALITAGLSDQISVTTAHSLSILKSSFPPSAGEFQPDLLDSLTPILEFHR 193

Query: 203 EIDSPFCLNAYPFLAYMSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQIDAAYAAL 262
           + DSPF +NAYPF AY  +P+ + +++ LF+  +GI DP T   YDNML AQIDA Y+AL
Sbjct: 194 KTDSPFLINAYPFFAYKGNPKEVPLDFVLFQPNQGIVDPATGFHYDNMLFAQIDAVYSAL 253

Query: 263 ADAGYHKMEVIVTETGWASRGDENEAAATANNAKIFNYNLRKRL--AKKKGTPFRPKNVL 322
           A AG+  + V ++ETGW S+GD++E  AT  NAK +N NL K +   KK  TP +P N L
Sbjct: 254 AAAGFKSLRVEISETGWPSKGDDDEVGATPENAKRYNGNLIKMMMSGKKTKTPLKPNNDL 313

Query: 323 KAYIFATFNENLKPGPTSERNFGLFKADGTISYDIGF 357
             Y+FA FNENLKPGPTSERN+GLFK DGT +Y +GF
Sbjct: 314 SIYVFALFNENLKPGPTSERNYGLFKPDGTQAYSLGF 349

BLAST of CmaCh12G011990 vs. TAIR 10
Match: AT2G27500.1 (Glycosyl hydrolase superfamily protein )

HSP 1 Score: 319.3 bits (817), Expect = 4.3e-87
Identity = 147/321 (45.79%), Postives = 218/321 (67.91%), Query Frame = 0

Query: 38  GINYGRIADNIPSPDEVVSLLKAAKIKNVRIYDADHKVLNAFSGTGLELVVGLPNGFLKE 97
           GINYG+IA+N+PSP  V  LL++  I  V++YDAD  VL +FS + ++ ++GL N +L+ 
Sbjct: 30  GINYGQIANNLPSPARVAVLLRSLNITRVKLYDADPNVLFSFSNSQVDFMIGLGNEYLQN 89

Query: 98  MSSSEERAMNWVKENVQAFLPQTHICGIAIGNEVLGGGDLELWGALLGATKNIYKAVKKL 157
           MS+   +A +W+++ ++  + +T I  I +GNE+    D  L  +LL A K++Y A+  L
Sbjct: 90  MSTDPTKAQDWLQQRLEPHISKTRITSIVVGNEIFKTNDHVLIQSLLPAMKSVYAALTNL 149

Query: 158 NLDGVIQITTAHSQAVFSNSFPPSSCIFRENVVQYMKPLLEFFSEIDSPFCLNAYPFLAY 217
            L+  + +T+AHS  + S S+PPSS  F+E  +QY++PLL+F S+I+SPF +NAYPF AY
Sbjct: 150 GLEKQVTVTSAHSLDILSTSYPPSSGSFKEEFIQYLQPLLDFHSQIESPFLINAYPFFAY 209

Query: 218 MSDPENIDINYALFESTEGIFDPKTNLRYDNMLDAQIDAAYAALADAGYHKMEVIVTETG 277
              P+ + + Y LF+  +G+ DP TNL YDNML AQ+DA Y+A+   G+  +EV ++ETG
Sbjct: 210 KDSPKEVPLEYVLFQPNQGMVDPNTNLHYDNMLFAQVDALYSAIKTLGHTDIEVRISETG 269

Query: 278 WASRGDENEAAATANNAKIFNYNLRKRLAKKKGTPFRPKNVLKAYIFATFNENLKPGPTS 337
           W S+GDENE  A+  NA ++N NL K + ++KGTP +    +  Y+FA FNENLKPGP S
Sbjct: 270 WPSKGDENEIGASPENAALYNGNLLKLIQQRKGTPAKQSVPIDVYVFALFNENLKPGPVS 329

Query: 338 ERNFGLFKADGTISYDIGFHG 359
           ERN+GLF  DG   Y++G  G
Sbjct: 330 ERNYGLFYPDGKPVYNVGMQG 350

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L8681.3e-8847.48Glucan endo-1,3-beta-glucosidase 11 OS=Arabidopsis thaliana OX=3702 GN=At1g32860... [more]
Q9ZQG96.1e-8645.79Glucan endo-1,3-beta-glucosidase 14 OS=Arabidopsis thaliana OX=3702 GN=At2g27500... [more]
Q9FHX52.7e-8144.44Glucan endo-1,3-beta-glucosidase 10 OS=Arabidopsis thaliana OX=3702 GN=At5g42100... [more]
Q9M0692.9e-8044.55Glucan endo-1,3-beta-glucosidase 7 OS=Arabidopsis thaliana OX=3702 GN=At4g34480 ... [more]
Q8VYE55.7e-7643.15Glucan endo-1,3-beta-glucosidase 12 OS=Arabidopsis thaliana OX=3702 GN=At4g29360... [more]
Match NameE-valueIdentityDescription
A0A6J1HML72.4e-226100.00(1->3)-beta-glucan endohydrolase OS=Cucurbita maxima OX=3661 GN=LOC111465570 PE=... [more]
A0A6J1FIR43.8e-22498.75(1->3)-beta-glucan endohydrolase OS=Cucurbita moschata OX=3662 GN=LOC111444294 P... [more]
A0A1S3C5G61.0e-20590.73(1->3)-beta-glucan endohydrolase OS=Cucumis melo OX=3656 GN=LOC103497110 PE=3 SV... [more]
A0A0A0LF782.8e-20389.97(1->3)-beta-glucan endohydrolase OS=Cucumis sativus OX=3659 GN=Csa_2G006850 PE=3... [more]
A0A6J1CPQ32.0e-20190.31(1->3)-beta-glucan endohydrolase OS=Momordica charantia OX=3673 GN=LOC111013168 ... [more]
Match NameE-valueIdentityDescription
XP_022965781.14.9e-226100.00glucan endo-1,3-beta-glucosidase 11-like [Cucurbita maxima][more]
XP_022938105.17.9e-22498.75glucan endo-1,3-beta-glucosidase 11-like [Cucurbita moschata][more]
KAG6586386.12.3e-22398.50Glucan endo-1,3-beta-glucosidase 11, partial [Cucurbita argyrosperma subsp. soro... [more]
XP_023537789.12.5e-22297.99glucan endo-1,3-beta-glucosidase 11-like [Cucurbita pepo subsp. pepo][more]
XP_008457422.12.2e-20590.73PREDICTED: glucan endo-1,3-beta-glucosidase 11 [Cucumis melo][more]
Match NameE-valueIdentityDescription
AT2G26600.11.6e-15069.79Glycosyl hydrolase superfamily protein [more]
AT3G15800.15.8e-13264.10Glycosyl hydrolase superfamily protein [more]
AT2G26600.27.6e-11668.77Glycosyl hydrolase superfamily protein [more]
AT1G32860.19.4e-9047.48Glycosyl hydrolase superfamily protein [more]
AT2G27500.14.3e-8745.79Glycosyl hydrolase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.20.20.80Glycosidasescoord: 36..362
e-value: 2.6E-115
score: 386.8
NoneNo IPR availablePANTHERPTHR32227:SF129GLYCOSYL HYDROLASE SUPERFAMILY PROTEINcoord: 27..390
IPR000490Glycoside hydrolase family 17PFAMPF00332Glyco_hydro_17coord: 38..356
e-value: 5.9E-79
score: 265.9
IPR044965Glycoside hydrolase family 17, plantPANTHERPTHR32227GLUCAN ENDO-1,3-BETA-GLUCOSIDASE BG1-RELATED-RELATEDcoord: 27..390
IPR017853Glycoside hydrolase superfamilySUPERFAMILY51445(Trans)glycosidasescoord: 37..355

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G011990.1CmaCh12G011990.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0005975 carbohydrate metabolic process
cellular_component GO:0046658 anchored component of plasma membrane
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0042973 glucan endo-1,3-beta-D-glucosidase activity
molecular_function GO:0004553 hydrolase activity, hydrolyzing O-glycosyl compounds