CmaCh12G011870 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G011870
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionsynaptotagmin-5-like
LocationCma_Chr12: 9303787 .. 9306470 (+)
RNA-Seq ExpressionCmaCh12G011870
SyntenyCmaCh12G011870
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGAAGACCAGCAAAATAATTGTGAGTTTATCTTACTTCTCTTAACTTCGTTCTCTCTTTCGTCTTTTGGATATACTATAAATTTCTATTGTTTCTTTTGTAGAACAATGATTTGAATCCAGTTTGGAATGAGCACTTTGAGTTTGTTGTTGAAGATGAATTGACACAACATTTGGTCGTGAAAGTTTATGACGATGAAGGACTTCAGGCTTCTGAGCTTATACAATGTGCTCAGATACGATTAAGCGAGCTTCAACTGGGTAGGGTGAAGGATGTGTGGTTGAAGCTGGTTAAGGATCTGGAAGTTCATAGAGATAATAAAAACAGGGGGCAGGTAACAACACCTTTAATGAACATTTTGCTTATTGGTGTCAAATTCCATTTATATGATTTATATGTGTGAGACCCCACATCGGTTGGAAAGGAGAACGAAACATTCTTTATAAGGGTGTGGAAACCTCTCCCTAGCATATGCATTTTTAAAAACCTTGTGGGGAAGCCGAAAGGAAAAGGCTAAAGAGGACAATATCTACGAGCGGTGGGCTTGAGCTATTACAAGTGACATCAGAGCTAGACACCAAGAGATGTGCCAGCAAGGAGGCTAAGCCTAAGCCCCAAAGGGGGTGGACACGAGGCAGTGTGCCAGTAAGGATGCTGGGCCCCGAAGGGGGGTGGATTGGAGGTTCCACATCGATTGGAGAAGGGAACGAGTGCTAATGAGGATGCTAGGTCCCGAAGGGGGGTAGATTGTGAGATCCCACATCGGTTGGGGAGGAGAACAAAACATACTTTATAAGAGCGTGGAAACCTCTCCCTTGCAGACATGTTTTAAAAACCTTAAGGGAAAGCTTGAAAAGGAAAGCCCAAAGAGAACAATATCTGCTAGTGGTGGGCTTAGGCCGTTACAATATGTATGAACAGTGTCTGTTCGAGTCCATGCTCATCCTCATTATAGTAAATCTTTCTCGTACTATCCACGTTCAACATTCTTGATGTTCATACCTCTATACTAAATCTTTCTCGTACTATCCACGTTCAACATTCTTGATGTTCATACCTCTATACTAAATCTTTCTCGTACTATCCACGTTCAACATTTTTGATGTTCATACCTCCATTAAGATTTTCGACATATTGTCTTCCCAGATACATTCCTGAGTAGTTCATAGTGATCTAAAGCAATCCCATGATTCTGCTAAGAAGACTTCCCATATATGATAGTGCATTCCTTTTGTGGTTTTCTGTTAAATGTGTTCATTAGGTGCACTTGGAGCTTCTATACTGTCCTTTCGGTATGGAAAGAGGCTTTACAAATCCATTTGCCTCTGATTTTTCAATGACTTCCTTGGAGAGTGTACTCAAGAGTCAGCCAAATGGAACGGAAGCTACCGAAAACGGACAAGCTGTCACACAGAAGAGGAAAGGGGTTATTATTAGAGGAGTACTTTCCGTGACTGTGATATCTGCAGAAGACTTGCCTGCAACAGATCTTGTAGGAAAGTCTGACCCATATGTTGTATTCACCATGAAAAAATCAGAAATGAAGAACAAAACAAGGGTAAAACTCCGATACCCGAAATTATGATCTAAAACTATCCCTGTTTGCCCTTTCCTTCTCCCTCTGTGATCTTTAGTTTCATATTTCAGGTTGTGAATGAGAGCTTAAATCCCATATGGAATCAGACTTTTGACTTTGTTGTTGAAGATGGACTACATGACATGCTTATAGCTGAAGTTTGGGATCACGACACTTTTGGAAAGGTGAAGCACCGTTTTATTAATACTGTCCATCTTTTTTGTCTCATTCAAGCTAAAATATAGTTGTGACTGGCTTCACCGAACATATACAGAATCTTTTAGTATTAGACCCCACATTGGTTGGGAAGGAGAACGAAACATCCTTTATAAGGGTGTGAAAACCTTTTCCTAGCAGACACGTTTTAAAATCCTTGAGGGGAAGCTTGAAAGGGAAAGCTCAAAGAGGACAATATCTACTAGCGGTGGGCCCGGACCATTATATTTAGCATGTCTTAATCTTTTAACTTGTTTTAGCACCTCTACTTCATGTTGTTGTGATCTCAGTCTAACCATTGCAACAATCACTAAAGATTATACAACTCAGAACTTGTTGCAACTTCCCCTTCACTAACATTGATTTCTTGGATCAATTAGGATTATATGGGCAGATGCATTTTGACACTTACAAGAGTAATATTAGAAGGGGAATACAAGGAATCGTTTGAACTCGATGGAGCCAAGTCAGGGCGGTTAAATCTACACCTCAAATGGATGCCACAGCCTATCTACCGTGATTCCTAAGCAATTCCCAGAACTGTAAACCAGTAAGTTGAGGATGTACAATACCTACTTCTCTGAGCAGATCAGTTGAACAATGAGTCTCACGTCTCTATCTGTGAGAGAAACGCATGAAAATTGTATATAATTGTTCGTCAGTAGTGAAGAGAGGGAATTACAAGCAGATGAAAGTAATGCCCAATACTTTAAATTCCTTTTAACTTTCTATTTTTCTTCTTTCTGGGCATATTGGTATCTAGGCTATTTTGAAGCGACTAGAATCTGAAAGGAAGCCTTCTTGGCAGCAAACTCTGAATGTTTTTGTGTGTCTTCTATTTGCAACCTCAAGAACATTTCTTTTAGCTGATTAAATATGCCAAGTTTTGGAT

mRNA sequence

ATGAAGACCAGCAAAATAATTAACAATGATTTGAATCCAGTTTGGAATGAGCACTTTGAGTTTGTTGTTGAAGATGAATTGACACAACATTTGGTCGTGAAAGTTTATGACGATGAAGGACTTCAGGCTTCTGAGCTTATACAATGTGCTCAGATACGATTAAGCGAGCTTCAACTGGGTAGGGTGAAGGATGTGTGGTTGAAGCTGGTTAAGGATCTGGAAGTTCATAGAGATAATAAAAACAGGGGGCAGGTGCACTTGGAGCTTCTATACTGTCCTTTCGGTATGGAAAGAGGCTTTACAAATCCATTTGCCTCTGATTTTTCAATGACTTCCTTGGAGAGTGTACTCAAGAGTCAGCCAAATGGAACGGAAGCTACCGAAAACGGACAAGCTGTCACACAGAAGAGGAAAGGGGTTATTATTAGAGGAGTACTTTCCGTGACTGTGATATCTGCAGAAGACTTGCCTGCAACAGATCTTGTAGGAAAGTCTGACCCATATGTTGTATTCACCATGAAAAAATCAGAAATGAAGAACAAAACAAGGGTTGTGAATGAGAGCTTAAATCCCATATGGAATCAGACTTTTGACTTTGTTGTTGAAGATGGACTACATGACATGCTTATAGCTGAAGTTTGGGATCACGACACTTTTGGAAAGGATTATATGGGCAGATGCATTTTGACACTTACAAGAGTAATATTAGAAGGGGAATACAAGGAATCGTTTGAACTCGATGGAGCCAAGTCAGGGCGGTTAAATCTACACCTCAAATGGATGCCACAGCCTATCTACCGTGATTCCTAAGCAATTCCCAGAACTGTAAACCAGTAAGTTGAGGATGTACAATACCTACTTCTCTGAGCAGATCAGTTGAACAATGAGTCTCACGTCTCTATCTGTGAGAGAAACGCATGAAAATTGTATATAATTGTTCGTCAGTAGTGAAGAGAGGGAATTACAAGCAGATGAAAGTAATGCCCAATACTTTAAATTCCTTTTAACTTTCTATTTTTCTTCTTTCTGGGCATATTGGTATCTAGGCTATTTTGAAGCGACTAGAATCTGAAAGGAAGCCTTCTTGGCAGCAAACTCTGAATGTTTTTGTGTGTCTTCTATTTGCAACCTCAAGAACATTTCTTTTAGCTGATTAAATATGCCAAGTTTTGGAT

Coding sequence (CDS)

ATGAAGACCAGCAAAATAATTAACAATGATTTGAATCCAGTTTGGAATGAGCACTTTGAGTTTGTTGTTGAAGATGAATTGACACAACATTTGGTCGTGAAAGTTTATGACGATGAAGGACTTCAGGCTTCTGAGCTTATACAATGTGCTCAGATACGATTAAGCGAGCTTCAACTGGGTAGGGTGAAGGATGTGTGGTTGAAGCTGGTTAAGGATCTGGAAGTTCATAGAGATAATAAAAACAGGGGGCAGGTGCACTTGGAGCTTCTATACTGTCCTTTCGGTATGGAAAGAGGCTTTACAAATCCATTTGCCTCTGATTTTTCAATGACTTCCTTGGAGAGTGTACTCAAGAGTCAGCCAAATGGAACGGAAGCTACCGAAAACGGACAAGCTGTCACACAGAAGAGGAAAGGGGTTATTATTAGAGGAGTACTTTCCGTGACTGTGATATCTGCAGAAGACTTGCCTGCAACAGATCTTGTAGGAAAGTCTGACCCATATGTTGTATTCACCATGAAAAAATCAGAAATGAAGAACAAAACAAGGGTTGTGAATGAGAGCTTAAATCCCATATGGAATCAGACTTTTGACTTTGTTGTTGAAGATGGACTACATGACATGCTTATAGCTGAAGTTTGGGATCACGACACTTTTGGAAAGGATTATATGGGCAGATGCATTTTGACACTTACAAGAGTAATATTAGAAGGGGAATACAAGGAATCGTTTGAACTCGATGGAGCCAAGTCAGGGCGGTTAAATCTACACCTCAAATGGATGCCACAGCCTATCTACCGTGATTCCTAA

Protein sequence

MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLGRVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQPNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKNKTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEYKESFELDGAKSGRLNLHLKWMPQPIYRDS
Homology
BLAST of CmaCh12G011870 vs. ExPASy Swiss-Prot
Match: Q8L706 (Synaptotagmin-5 OS=Arabidopsis thaliana OX=3702 GN=SYT5 PE=2 SV=1)

HSP 1 Score: 399.8 bits (1026), Expect = 2.4e-110
Identity = 193/268 (72.01%), Postives = 225/268 (83.96%), Query Frame = 0

Query: 2   KTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLGR 61
           K SK INNDLNP+WNEHFEFVVED  TQHLVV++YDDEG+QASELI CAQIRL EL+ G+
Sbjct: 299 KRSKTINNDLNPIWNEHFEFVVEDASTQHLVVRIYDDEGVQASELIGCAQIRLCELEPGK 358

Query: 62  VKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQP 121
           VKDVWLKLVKDLE+ RD KNRG+VHLELLY P+G   G  NPF +  SMTSLE VLK+  
Sbjct: 359 VKDVWLKLVKDLEIQRDTKNRGEVHLELLYIPYGSGNGIVNPFVTS-SMTSLERVLKN-- 418

Query: 122 NGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKNK 181
              + T+   A ++KRK VI+RGVLSVTVISAE++P  DL+GK+DPYVV +MKKS  K+K
Sbjct: 419 ---DTTDEENASSRKRKDVIVRGVLSVTVISAEEIPIQDLMGKADPYVVLSMKKSGAKSK 478

Query: 182 TRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEYK 241
           TRVVN+SLNP+WNQTFDFVVEDGLHDML+ EVWDHDTFGKDY+GRCILTLTRVI+E EYK
Sbjct: 479 TRVVNDSLNPVWNQTFDFVVEDGLHDMLVLEVWDHDTFGKDYIGRCILTLTRVIMEEEYK 538

Query: 242 ESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           + + LD +K+G+L LHLKWM Q IYRDS
Sbjct: 539 DWYPLDESKTGKLQLHLKWMAQSIYRDS 560

BLAST of CmaCh12G011870 vs. ExPASy Swiss-Prot
Match: A0JJX5 (Synaptotagmin-4 OS=Arabidopsis thaliana OX=3702 GN=SYT4 PE=2 SV=1)

HSP 1 Score: 371.7 bits (953), Expect = 7.0e-102
Identity = 175/268 (65.30%), Postives = 217/268 (80.97%), Query Frame = 0

Query: 2   KTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLGR 61
           K +K I+N LNP+WNEHFEF+VED  TQHL V+V+DDEG+ +S+LI  AQ+ L+EL  G+
Sbjct: 301 KKTKTISNSLNPIWNEHFEFIVEDVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGK 360

Query: 62  VKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQP 121
           VKD+WLKLVKDLE+ RD KNRGQV LELLYCP G E G  NPF  D+S+T LE VLK + 
Sbjct: 361 VKDIWLKLVKDLEIQRDTKNRGQVQLELLYCPLGKEGGLKNPFNPDYSLTILEKVLKPES 420

Query: 122 NGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKNK 181
             ++AT+  + VT K+K VI+RGVLSVTV++AEDLPA D +GK+D +VV T+KKSE K+K
Sbjct: 421 EDSDATDMKKLVTSKKKDVIVRGVLSVTVVAAEDLPAVDFMGKADAFVVITLKKSETKSK 480

Query: 182 TRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEYK 241
           TRVV +SLNP+WNQTFDFVVED LHD+L  EVWDHD FGKD +GR I+TLTRV+LEGE++
Sbjct: 481 TRVVPDSLNPVWNQTFDFVVEDALHDLLTLEVWDHDKFGKDKIGRVIMTLTRVMLEGEFQ 540

Query: 242 ESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           E FELDGAKSG+L +HLKW P+   RD+
Sbjct: 541 EWFELDGAKSGKLCVHLKWTPRLKLRDA 568

BLAST of CmaCh12G011870 vs. ExPASy Swiss-Prot
Match: D4ABL6 (Multiple C2 and transmembrane domain-containing protein 1 OS=Rattus norvegicus OX=10116 GN=Mctp1 PE=1 SV=4)

HSP 1 Score: 105.1 bits (261), Expect = 1.2e-21
Identity = 81/247 (32.79%), Postives = 130/247 (52.63%), Query Frame = 0

Query: 4   SKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYD-DEGLQASELIQCAQIRLSELQLGRV 63
           SKII+ +LNPVW E    V+ D L + L +KV+D D GLQ  + +  A + L++L+L R 
Sbjct: 291 SKIIHKNLNPVWEEK-ACVLIDHLREPLYIKVFDYDFGLQ-DDFMGSAFLDLTQLELNRP 350

Query: 64  KDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQPN 123
            DV L L    + H  + + G + L ++  P   E         D +M   +S  +S   
Sbjct: 351 TDVTLTL---KDPHYPDHDLGIILLSVILTPKEGE-------PRDVTMLMRKSWKRSSKF 410

Query: 124 GTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKNKT 183
            T++    +   Q RK  + RG++S+T+I   DL A D  G SDPYV F +     K K+
Sbjct: 411 QTQSL---RLSDQHRKSHLWRGIVSITLIEGRDLKAMDSNGLSDPYVKFRL--GHQKYKS 470

Query: 184 RVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGK--DYMGRCILTLTRVILEGEY 243
           +++ ++LNP W + FDF + +    ++    WD D  GK  D++GRC + L+ +  E  +
Sbjct: 471 KIMPKTLNPQWREQFDFHLYEERGGVMDITAWDKDA-GKRDDFIGRCQVDLSSLSREQTH 519

Query: 244 KESFELD 248
           K   +L+
Sbjct: 531 KLELQLE 519

BLAST of CmaCh12G011870 vs. ExPASy Swiss-Prot
Match: E9PV86 (Multiple C2 and transmembrane domain-containing protein 1 OS=Mus musculus OX=10090 GN=Mctp1 PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 1.6e-21
Identity = 81/247 (32.79%), Postives = 129/247 (52.23%), Query Frame = 0

Query: 4   SKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYD-DEGLQASELIQCAQIRLSELQLGRV 63
           SKII+ +LNPVW E    V+ D L + L +KV+D D GLQ  + +  A + L++L+L R 
Sbjct: 296 SKIIHKNLNPVWEEK-ACVLIDHLREPLYIKVFDYDFGLQ-DDFMGSAFLDLTQLELNRS 355

Query: 64  KDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQPN 123
            DV L L    + H  + + G + L ++  P   E         D +M   +S  +S   
Sbjct: 356 TDVTLTL---KDPHYPDHDLGIILLSVILTPKEGEH-------RDVTMLMRKSWKRSSKF 415

Query: 124 GTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKNKT 183
            T++    +   Q RK  + RG++S+T+I   DL A D  G SDPYV F +     K K+
Sbjct: 416 QTQSL---RLSDQHRKSHLWRGIVSITLIEGRDLKAMDSNGLSDPYVKFRL--GHQKYKS 475

Query: 184 RVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGK--DYMGRCILTLTRVILEGEY 243
           +++ ++LNP W + FDF + +    ++    WD D  GK  D++GRC + L+ +  E  +
Sbjct: 476 KIMPKTLNPQWREQFDFHLYEERGGIMDITAWDKDA-GKRDDFIGRCQVDLSSLSREQTH 524

Query: 244 KESFELD 248
           K    L+
Sbjct: 536 KLELHLE 524

BLAST of CmaCh12G011870 vs. ExPASy Swiss-Prot
Match: Q3U7R1 (Extended synaptotagmin-1 OS=Mus musculus OX=10090 GN=Esyt1 PE=1 SV=2)

HSP 1 Score: 104.4 bits (259), Expect = 2.1e-21
Identity = 89/275 (32.36%), Postives = 131/275 (47.64%), Query Frame = 0

Query: 2   KTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDE---GLQASELIQCAQIRLSELQ 61
           + SK   +  +PVW E F F ++D  +Q L V+V DD     L A  L     +  SEL 
Sbjct: 502 RESKATYSTNSPVWEEAFRFFLQDPRSQELDVQVKDDSRALTLGALTLPLARLLTASELT 561

Query: 62  LGRVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLE--SV 121
           L    D W +L        +++   ++ + +LY  +   R  T P A D+   SLE  S 
Sbjct: 562 L----DQWFQLSSS---GPNSRLYMKLVMRILYLDYSEIRFPTVPGAQDWDRESLETGSS 621

Query: 122 LKSQPNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATD------LVGKSDPYVV 181
           + + P     T N    T+         VL + V+ A+DL A D      + GKSDPYV 
Sbjct: 622 VDAPPRPYHTTPNSHFGTE--------NVLRIHVLEAQDLIAKDRFLGGLVKGKSDPYV- 681

Query: 182 FTMKKSEMKNKTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILT 241
             +K +    +T VV E LNP WN+ F+ +V       L  EV+D D    D++GR  ++
Sbjct: 682 -KLKVAGKSFRTHVVREDLNPRWNEVFEVIVTSIPGQELEIEVFDKDLDKDDFLGRYKVS 741

Query: 242 LTRVILEGEYKESFELDGAKSGRLNLHLKWM-PQP 265
           LT V+  G   E   L+   SGRL+L L+ + P+P
Sbjct: 742 LTTVLNSGFLDEWLTLEDVPSGRLHLRLERLTPRP 759

BLAST of CmaCh12G011870 vs. ExPASy TrEMBL
Match: A0A6J1FIX7 (synaptotagmin-5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111444332 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 1.7e-146
Identity = 259/269 (96.28%), Postives = 261/269 (97.03%), Query Frame = 0

Query: 1   MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLG 60
           MKTSKIINNDLNP+WNEHFEFVVEDE TQHLVVKVYDDEGLQASELI CAQIRLSELQ G
Sbjct: 291 MKTSKIINNDLNPIWNEHFEFVVEDESTQHLVVKVYDDEGLQASELIGCAQIRLSELQPG 350

Query: 61  RVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQ 120
           +VKDVWLKLVKDLEVHRDNKNRGQVHLEL YCPFGME GFTNPFASDF MTSLESVLKSQ
Sbjct: 351 KVKDVWLKLVKDLEVHRDNKNRGQVHLELQYCPFGMESGFTNPFASDFPMTSLESVLKSQ 410

Query: 121 PNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 180
            NGTEATENGQAVT KRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN
Sbjct: 411 ANGTEATENGQAVTPKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 470

Query: 181 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 240
           KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY
Sbjct: 471 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 530

Query: 241 KESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           KESFELDGAKSGRLNLHLKWMPQPIYRDS
Sbjct: 531 KESFELDGAKSGRLNLHLKWMPQPIYRDS 559

BLAST of CmaCh12G011870 vs. ExPASy TrEMBL
Match: A0A6J1FDA0 (synaptotagmin-5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444332 PE=3 SV=1)

HSP 1 Score: 528.5 bits (1360), Expect = 1.7e-146
Identity = 259/269 (96.28%), Postives = 261/269 (97.03%), Query Frame = 0

Query: 1   MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLG 60
           MKTSKIINNDLNP+WNEHFEFVVEDE TQHLVVKVYDDEGLQASELI CAQIRLSELQ G
Sbjct: 299 MKTSKIINNDLNPIWNEHFEFVVEDESTQHLVVKVYDDEGLQASELIGCAQIRLSELQPG 358

Query: 61  RVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQ 120
           +VKDVWLKLVKDLEVHRDNKNRGQVHLEL YCPFGME GFTNPFASDF MTSLESVLKSQ
Sbjct: 359 KVKDVWLKLVKDLEVHRDNKNRGQVHLELQYCPFGMESGFTNPFASDFPMTSLESVLKSQ 418

Query: 121 PNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 180
            NGTEATENGQAVT KRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN
Sbjct: 419 ANGTEATENGQAVTPKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 478

Query: 181 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 240
           KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY
Sbjct: 479 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 538

Query: 241 KESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           KESFELDGAKSGRLNLHLKWMPQPIYRDS
Sbjct: 539 KESFELDGAKSGRLNLHLKWMPQPIYRDS 567

BLAST of CmaCh12G011870 vs. ExPASy TrEMBL
Match: A0A6J1KFU6 (synaptotagmin-5-like OS=Cucurbita maxima OX=3661 GN=LOC111493519 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.2e-141
Identity = 250/269 (92.94%), Postives = 258/269 (95.91%), Query Frame = 0

Query: 1   MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLG 60
           MKTSKIINNDLNPVWNEHFEFVVEDE TQ+LVVKVYDDEGLQASELI CAQ+RLSELQ G
Sbjct: 299 MKTSKIINNDLNPVWNEHFEFVVEDESTQNLVVKVYDDEGLQASELIGCAQMRLSELQPG 358

Query: 61  RVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQ 120
           +VKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGME GFTNPFA DFSMTSLESVLKS+
Sbjct: 359 KVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMENGFTNPFAPDFSMTSLESVLKSR 418

Query: 121 PNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 180
            NGTEATE+ QA TQKRK VIIRGVL VTVISAEDLPATD+VGKSDPYVV TMKKSEMKN
Sbjct: 419 TNGTEATESEQAATQKRKEVIIRGVLCVTVISAEDLPATDMVGKSDPYVVLTMKKSEMKN 478

Query: 181 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 240
           KTRVVNESLNP+WNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY
Sbjct: 479 KTRVVNESLNPVWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 538

Query: 241 KESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           KESFELDGAKSGRLNLHLKWMPQPIYRD+
Sbjct: 539 KESFELDGAKSGRLNLHLKWMPQPIYRDT 567

BLAST of CmaCh12G011870 vs. ExPASy TrEMBL
Match: A0A6J1G398 (synaptotagmin-5-like OS=Cucurbita moschata OX=3662 GN=LOC111450427 PE=3 SV=1)

HSP 1 Score: 512.3 bits (1318), Expect = 1.2e-141
Identity = 250/269 (92.94%), Postives = 258/269 (95.91%), Query Frame = 0

Query: 1   MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLG 60
           MKTSKIINNDLNPVWNEHFEFVVEDE TQ+LVVKVYDDEGLQASELI CAQ+RLSELQ G
Sbjct: 299 MKTSKIINNDLNPVWNEHFEFVVEDESTQNLVVKVYDDEGLQASELIGCAQMRLSELQPG 358

Query: 61  RVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQ 120
           +VKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGME GFTNPFA DFSMTSLESVLKS+
Sbjct: 359 KVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMENGFTNPFAPDFSMTSLESVLKSR 418

Query: 121 PNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 180
            NGTEATE+ QA TQKRK VIIRGVL VTVISAEDLPATD+VGKSDPYVV TMKKSEMKN
Sbjct: 419 TNGTEATESEQAATQKRKEVIIRGVLCVTVISAEDLPATDMVGKSDPYVVLTMKKSEMKN 478

Query: 181 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 240
           KTRVVNESLNP+WNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY
Sbjct: 479 KTRVVNESLNPVWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 538

Query: 241 KESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           KESFELDGAKSGRLNLHLKWMPQPIYRD+
Sbjct: 539 KESFELDGAKSGRLNLHLKWMPQPIYRDT 567

BLAST of CmaCh12G011870 vs. ExPASy TrEMBL
Match: A0A0A0LI51 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G008060 PE=3 SV=1)

HSP 1 Score: 509.6 bits (1311), Expect = 7.9e-141
Identity = 251/269 (93.31%), Postives = 257/269 (95.54%), Query Frame = 0

Query: 1   MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLG 60
           MKTSKIINNDLNPVWNEHFEFVVEDE TQHLVVKVYDDEGLQASELI CAQI+LSELQ G
Sbjct: 299 MKTSKIINNDLNPVWNEHFEFVVEDESTQHLVVKVYDDEGLQASELIGCAQIQLSELQPG 358

Query: 61  RVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQ 120
           +VKDVWLKLVKDLEV RDNKNRGQVHLELLYCPFGME GFTNPFASDF MTSLESVLK++
Sbjct: 359 KVKDVWLKLVKDLEVIRDNKNRGQVHLELLYCPFGMENGFTNPFASDFRMTSLESVLKNR 418

Query: 121 PNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 180
            NGTEATE+ QAVTQKRK VIIRGVLSVTVISAEDLPATDLVGKSDPYVV TMKKS MKN
Sbjct: 419 ANGTEATESEQAVTQKRKEVIIRGVLSVTVISAEDLPATDLVGKSDPYVVLTMKKSGMKN 478

Query: 181 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 240
           KTRVVNESLNPIWNQTFDFVVEDGLHDMLI EVWDHDTFGKDYMGRCILTLTRVILEGEY
Sbjct: 479 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIVEVWDHDTFGKDYMGRCILTLTRVILEGEY 538

Query: 241 KESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           KESFELDGAKSGRLNLHLKWMPQPIYRD+
Sbjct: 539 KESFELDGAKSGRLNLHLKWMPQPIYRDT 567

BLAST of CmaCh12G011870 vs. NCBI nr
Match: KAG6586373.1 (Synaptotagmin-5, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 530.8 bits (1366), Expect = 6.9e-147
Identity = 260/269 (96.65%), Postives = 262/269 (97.40%), Query Frame = 0

Query: 1   MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLG 60
           MKTSKIINNDLNP+WNEHFEFVVEDE TQHLVVKVYDDEGLQASELI CAQIRLSELQ G
Sbjct: 291 MKTSKIINNDLNPIWNEHFEFVVEDESTQHLVVKVYDDEGLQASELIGCAQIRLSELQPG 350

Query: 61  RVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQ 120
           +VKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGME GFTNPFASDF MTSLESVLKSQ
Sbjct: 351 KVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMESGFTNPFASDFPMTSLESVLKSQ 410

Query: 121 PNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 180
            NGTEATENGQAVT KRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN
Sbjct: 411 ANGTEATENGQAVTPKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 470

Query: 181 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 240
           KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY
Sbjct: 471 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 530

Query: 241 KESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           KESFELDGAKSGRLNLHLKWMPQPIYRDS
Sbjct: 531 KESFELDGAKSGRLNLHLKWMPQPIYRDS 559

BLAST of CmaCh12G011870 vs. NCBI nr
Match: XP_022938165.1 (synaptotagmin-5-like isoform X2 [Cucurbita moschata])

HSP 1 Score: 528.5 bits (1360), Expect = 3.4e-146
Identity = 259/269 (96.28%), Postives = 261/269 (97.03%), Query Frame = 0

Query: 1   MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLG 60
           MKTSKIINNDLNP+WNEHFEFVVEDE TQHLVVKVYDDEGLQASELI CAQIRLSELQ G
Sbjct: 291 MKTSKIINNDLNPIWNEHFEFVVEDESTQHLVVKVYDDEGLQASELIGCAQIRLSELQPG 350

Query: 61  RVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQ 120
           +VKDVWLKLVKDLEVHRDNKNRGQVHLEL YCPFGME GFTNPFASDF MTSLESVLKSQ
Sbjct: 351 KVKDVWLKLVKDLEVHRDNKNRGQVHLELQYCPFGMESGFTNPFASDFPMTSLESVLKSQ 410

Query: 121 PNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 180
            NGTEATENGQAVT KRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN
Sbjct: 411 ANGTEATENGQAVTPKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 470

Query: 181 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 240
           KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY
Sbjct: 471 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 530

Query: 241 KESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           KESFELDGAKSGRLNLHLKWMPQPIYRDS
Sbjct: 531 KESFELDGAKSGRLNLHLKWMPQPIYRDS 559

BLAST of CmaCh12G011870 vs. NCBI nr
Match: XP_022938164.1 (synaptotagmin-5-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 528.5 bits (1360), Expect = 3.4e-146
Identity = 259/269 (96.28%), Postives = 261/269 (97.03%), Query Frame = 0

Query: 1   MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLG 60
           MKTSKIINNDLNP+WNEHFEFVVEDE TQHLVVKVYDDEGLQASELI CAQIRLSELQ G
Sbjct: 299 MKTSKIINNDLNPIWNEHFEFVVEDESTQHLVVKVYDDEGLQASELIGCAQIRLSELQPG 358

Query: 61  RVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQ 120
           +VKDVWLKLVKDLEVHRDNKNRGQVHLEL YCPFGME GFTNPFASDF MTSLESVLKSQ
Sbjct: 359 KVKDVWLKLVKDLEVHRDNKNRGQVHLELQYCPFGMESGFTNPFASDFPMTSLESVLKSQ 418

Query: 121 PNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 180
            NGTEATENGQAVT KRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN
Sbjct: 419 ANGTEATENGQAVTPKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 478

Query: 181 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 240
           KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY
Sbjct: 479 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 538

Query: 241 KESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           KESFELDGAKSGRLNLHLKWMPQPIYRDS
Sbjct: 539 KESFELDGAKSGRLNLHLKWMPQPIYRDS 567

BLAST of CmaCh12G011870 vs. NCBI nr
Match: XP_023537352.1 (synaptotagmin-5-like isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 528.1 bits (1359), Expect = 4.5e-146
Identity = 259/269 (96.28%), Postives = 262/269 (97.40%), Query Frame = 0

Query: 1   MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLG 60
           MKTSKIINNDLNPVWNEHFEFVVEDE TQHLVVKVYDDEGLQASELI CAQIRLS+LQ G
Sbjct: 291 MKTSKIINNDLNPVWNEHFEFVVEDESTQHLVVKVYDDEGLQASELIGCAQIRLSKLQPG 350

Query: 61  RVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQ 120
           +VKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGME GFTNPFASDF MTSLESVLKS+
Sbjct: 351 KVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMESGFTNPFASDFPMTSLESVLKSR 410

Query: 121 PNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 180
            NGTEATENGQAVT KRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN
Sbjct: 411 ANGTEATENGQAVTPKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 470

Query: 181 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 240
           KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY
Sbjct: 471 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 530

Query: 241 KESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           KESFELDGAKSGRLNLHLKWMPQPIYRDS
Sbjct: 531 KESFELDGAKSGRLNLHLKWMPQPIYRDS 559

BLAST of CmaCh12G011870 vs. NCBI nr
Match: XP_023537351.1 (synaptotagmin-5-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 528.1 bits (1359), Expect = 4.5e-146
Identity = 259/269 (96.28%), Postives = 262/269 (97.40%), Query Frame = 0

Query: 1   MKTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLG 60
           MKTSKIINNDLNPVWNEHFEFVVEDE TQHLVVKVYDDEGLQASELI CAQIRLS+LQ G
Sbjct: 299 MKTSKIINNDLNPVWNEHFEFVVEDESTQHLVVKVYDDEGLQASELIGCAQIRLSKLQPG 358

Query: 61  RVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQ 120
           +VKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGME GFTNPFASDF MTSLESVLKS+
Sbjct: 359 KVKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMESGFTNPFASDFPMTSLESVLKSR 418

Query: 121 PNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 180
            NGTEATENGQAVT KRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN
Sbjct: 419 ANGTEATENGQAVTPKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKN 478

Query: 181 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 240
           KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY
Sbjct: 479 KTRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEY 538

Query: 241 KESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           KESFELDGAKSGRLNLHLKWMPQPIYRDS
Sbjct: 539 KESFELDGAKSGRLNLHLKWMPQPIYRDS 567

BLAST of CmaCh12G011870 vs. TAIR 10
Match: AT1G05500.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 399.8 bits (1026), Expect = 1.7e-111
Identity = 193/268 (72.01%), Postives = 225/268 (83.96%), Query Frame = 0

Query: 2   KTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLGR 61
           K SK INNDLNP+WNEHFEFVVED  TQHLVV++YDDEG+QASELI CAQIRL EL+ G+
Sbjct: 299 KRSKTINNDLNPIWNEHFEFVVEDASTQHLVVRIYDDEGVQASELIGCAQIRLCELEPGK 358

Query: 62  VKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQP 121
           VKDVWLKLVKDLE+ RD KNRG+VHLELLY P+G   G  NPF +  SMTSLE VLK+  
Sbjct: 359 VKDVWLKLVKDLEIQRDTKNRGEVHLELLYIPYGSGNGIVNPFVTS-SMTSLERVLKN-- 418

Query: 122 NGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKNK 181
              + T+   A ++KRK VI+RGVLSVTVISAE++P  DL+GK+DPYVV +MKKS  K+K
Sbjct: 419 ---DTTDEENASSRKRKDVIVRGVLSVTVISAEEIPIQDLMGKADPYVVLSMKKSGAKSK 478

Query: 182 TRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEYK 241
           TRVVN+SLNP+WNQTFDFVVEDGLHDML+ EVWDHDTFGKDY+GRCILTLTRVI+E EYK
Sbjct: 479 TRVVNDSLNPVWNQTFDFVVEDGLHDMLVLEVWDHDTFGKDYIGRCILTLTRVIMEEEYK 538

Query: 242 ESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           + + LD +K+G+L LHLKWM Q IYRDS
Sbjct: 539 DWYPLDESKTGKLQLHLKWMAQSIYRDS 560

BLAST of CmaCh12G011870 vs. TAIR 10
Match: AT5G11100.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 371.7 bits (953), Expect = 5.0e-103
Identity = 175/268 (65.30%), Postives = 217/268 (80.97%), Query Frame = 0

Query: 2   KTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLGR 61
           K +K I+N LNP+WNEHFEF+VED  TQHL V+V+DDEG+ +S+LI  AQ+ L+EL  G+
Sbjct: 301 KKTKTISNSLNPIWNEHFEFIVEDVSTQHLTVRVFDDEGVGSSQLIGAAQVPLNELVPGK 360

Query: 62  VKDVWLKLVKDLEVHRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKSQP 121
           VKD+WLKLVKDLE+ RD KNRGQV LELLYCP G E G  NPF  D+S+T LE VLK + 
Sbjct: 361 VKDIWLKLVKDLEIQRDTKNRGQVQLELLYCPLGKEGGLKNPFNPDYSLTILEKVLKPES 420

Query: 122 NGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEMKNK 181
             ++AT+  + VT K+K VI+RGVLSVTV++AEDLPA D +GK+D +VV T+KKSE K+K
Sbjct: 421 EDSDATDMKKLVTSKKKDVIVRGVLSVTVVAAEDLPAVDFMGKADAFVVITLKKSETKSK 480

Query: 182 TRVVNESLNPIWNQTFDFVVEDGLHDMLIAEVWDHDTFGKDYMGRCILTLTRVILEGEYK 241
           TRVV +SLNP+WNQTFDFVVED LHD+L  EVWDHD FGKD +GR I+TLTRV+LEGE++
Sbjct: 481 TRVVPDSLNPVWNQTFDFVVEDALHDLLTLEVWDHDKFGKDKIGRVIMTLTRVMLEGEFQ 540

Query: 242 ESFELDGAKSGRLNLHLKWMPQPIYRDS 270
           E FELDGAKSG+L +HLKW P+   RD+
Sbjct: 541 EWFELDGAKSGKLCVHLKWTPRLKLRDA 568

BLAST of CmaCh12G011870 vs. TAIR 10
Match: AT5G04220.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 92.0 bits (227), Expect = 7.6e-19
Identity = 68/267 (25.47%), Postives = 131/267 (49.06%), Query Frame = 0

Query: 2   KTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLGR 61
           K + I   +LNP WNEHF+ +V+D  +Q L ++V+D + +   + +    I L ++  G 
Sbjct: 76  KKTTIKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKINPGE 135

Query: 62  VKDVWLKLVKDLEV---HRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLK 121
            K+  L L+K+  V     D K RG++ ++L Y PF  E              S++   +
Sbjct: 136 RKEFNLDLIKNSNVVMDSGDKKKRGRLEVDLRYVPFREE--------------SIKR--R 195

Query: 122 SQPNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEM 181
            +    +++E+   ++Q        G+LSV V SA+D+        S+PY V   +    
Sbjct: 196 KESREEKSSEDDDFLSQ-------AGLLSVAVQSAKDVEGKK--KHSNPYAVVLFRGE-- 255

Query: 182 KNKTRVVNESLNPIWNQTFDFVVED-GLHDMLIAEVWDHDT----FGKDYMGRCILTLTR 241
           K KT+++ ++ +P WN+ F F +E+  + + +  EV    T      K+ +G   + L  
Sbjct: 256 KKKTKMLKKTRDPRWNEEFQFTLEEPPVKESIRVEVMSKGTGFHFRSKEELGHVDINLDD 315

Query: 242 VILEGEYKESFELDGAKSGRLNLHLKW 261
           V+  G   + + L  +++G +++ ++W
Sbjct: 316 VVDNGRINQKYHLINSRNGIIHIEIRW 315

BLAST of CmaCh12G011870 vs. TAIR 10
Match: AT5G04220.2 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 92.0 bits (227), Expect = 7.6e-19
Identity = 68/267 (25.47%), Postives = 131/267 (49.06%), Query Frame = 0

Query: 2   KTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLGR 61
           K + I   +LNP WNEHF+ +V+D  +Q L ++V+D + +   + +    I L ++  G 
Sbjct: 298 KKTTIKKRNLNPEWNEHFKLIVKDPNSQVLQLEVFDWDKVGGHDRLGMQMIPLQKINPGE 357

Query: 62  VKDVWLKLVKDLEV---HRDNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLK 121
            K+  L L+K+  V     D K RG++ ++L Y PF  E              S++   +
Sbjct: 358 RKEFNLDLIKNSNVVMDSGDKKKRGRLEVDLRYVPFREE--------------SIKR--R 417

Query: 122 SQPNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGKSDPYVVFTMKKSEM 181
            +    +++E+   ++Q        G+LSV V SA+D+        S+PY V   +    
Sbjct: 418 KESREEKSSEDDDFLSQ-------AGLLSVAVQSAKDVEGKK--KHSNPYAVVLFRGE-- 477

Query: 182 KNKTRVVNESLNPIWNQTFDFVVED-GLHDMLIAEVWDHDT----FGKDYMGRCILTLTR 241
           K KT+++ ++ +P WN+ F F +E+  + + +  EV    T      K+ +G   + L  
Sbjct: 478 KKKTKMLKKTRDPRWNEEFQFTLEEPPVKESIRVEVMSKGTGFHFRSKEELGHVDINLDD 537

Query: 242 VILEGEYKESFELDGAKSGRLNLHLKW 261
           V+  G   + + L  +++G +++ ++W
Sbjct: 538 VVDNGRINQKYHLINSRNGIIHIEIRW 537

BLAST of CmaCh12G011870 vs. TAIR 10
Match: AT1G20080.1 (Calcium-dependent lipid-binding (CaLB domain) family protein )

HSP 1 Score: 83.6 bits (205), Expect = 2.7e-16
Identity = 74/267 (27.72%), Postives = 124/267 (46.44%), Query Frame = 0

Query: 2   KTSKIINNDLNPVWNEHFEFVVEDELTQHLVVKVYDDEGLQASELIQCAQIRLSELQLGR 61
           K + + +++LNP WNE F+ VV++  +Q L + VYD E +   + I    I+L +L    
Sbjct: 297 KKTVVKHSNLNPEWNEEFDLVVKEPESQELQLIVYDWEQVGKHDKIGMNVIQLKDLTPEE 356

Query: 62  VKDVWLKLVKDLEVHR--DNKNRGQVHLELLYCPFGMERGFTNPFASDFSMTSLESVLKS 121
            K + L+L+K +E       K+RGQ+ +E+ Y           PF  D    +++     
Sbjct: 357 PKLMTLELLKSMEPKEPVSEKSRGQLVVEVEY----------KPFKDDDIPENID----- 416

Query: 122 QPNGTEATENGQAVTQKRKGVIIRGVLSVTVISAEDLPATDLVGK--SDPYVVFTMKKSE 181
            PN  E    G   T         G+L V V  AEDL      GK  ++P V    +  E
Sbjct: 417 DPNAVEKAPEGTPST--------GGLLVVIVHEAEDLE-----GKYHTNPSVRLLFRGEE 476

Query: 182 MKNKTRVVNESLNPIWNQTFDFVV-EDGLHDMLIAEVWDHDT---FGKDYMGRCILTLTR 241
              KT+ V ++  P W++ F F + E  ++D L  EV    +     K+ +G  ++ L  
Sbjct: 477 --RKTKRVKKNREPRWDEDFQFPLDEPPINDKLHVEVISSSSRLIHPKETLGYVVINLGD 533

Query: 242 VILEGEYKESFELDGAKSGRLNLHLKW 261
           V+      + + L  +K+GR+ + L+W
Sbjct: 537 VVSNRRINDKYHLIDSKNGRIQIELQW 533

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8L7062.4e-11072.01Synaptotagmin-5 OS=Arabidopsis thaliana OX=3702 GN=SYT5 PE=2 SV=1[more]
A0JJX57.0e-10265.30Synaptotagmin-4 OS=Arabidopsis thaliana OX=3702 GN=SYT4 PE=2 SV=1[more]
D4ABL61.2e-2132.79Multiple C2 and transmembrane domain-containing protein 1 OS=Rattus norvegicus O... [more]
E9PV861.6e-2132.79Multiple C2 and transmembrane domain-containing protein 1 OS=Mus musculus OX=100... [more]
Q3U7R12.1e-2132.36Extended synaptotagmin-1 OS=Mus musculus OX=10090 GN=Esyt1 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1FIX71.7e-14696.28synaptotagmin-5-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111444332 PE... [more]
A0A6J1FDA01.7e-14696.28synaptotagmin-5-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444332 PE... [more]
A0A6J1KFU61.2e-14192.94synaptotagmin-5-like OS=Cucurbita maxima OX=3661 GN=LOC111493519 PE=3 SV=1[more]
A0A6J1G3981.2e-14192.94synaptotagmin-5-like OS=Cucurbita moschata OX=3662 GN=LOC111450427 PE=3 SV=1[more]
A0A0A0LI517.9e-14193.31Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G008060 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
KAG6586373.16.9e-14796.65Synaptotagmin-5, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022938165.13.4e-14696.28synaptotagmin-5-like isoform X2 [Cucurbita moschata][more]
XP_022938164.13.4e-14696.28synaptotagmin-5-like isoform X1 [Cucurbita moschata][more]
XP_023537352.14.5e-14696.28synaptotagmin-5-like isoform X2 [Cucurbita pepo subsp. pepo][more]
XP_023537351.14.5e-14696.28synaptotagmin-5-like isoform X1 [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G05500.11.7e-11172.01Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT5G11100.15.0e-10365.30Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT5G04220.17.6e-1925.47Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT5G04220.27.6e-1925.47Calcium-dependent lipid-binding (CaLB domain) family protein [more]
AT1G20080.12.7e-1627.72Calcium-dependent lipid-binding (CaLB domain) family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000008C2 domainPRINTSPR00360C2DOMAINcoord: 187..200
score: 45.85
coord: 209..217
score: 49.22
coord: 161..173
score: 43.74
IPR000008C2 domainSMARTSM00239C2_3ccoord: 3..66
e-value: 0.018
score: 24.2
coord: 145..243
e-value: 4.5E-24
score: 96.0
IPR000008C2 domainPFAMPF00168C2coord: 3..69
e-value: 5.4E-13
score: 49.1
coord: 144..245
e-value: 6.5E-25
score: 87.4
IPR000008C2 domainPROSITEPS50004C2coord: 1..67
score: 13.986746
IPR000008C2 domainPROSITEPS50004C2coord: 127..244
score: 20.662346
IPR035892C2 domain superfamilyGENE3D2.60.40.150C2 domaincoord: 1..93
e-value: 2.9E-18
score: 68.1
coord: 128..262
e-value: 3.4E-35
score: 123.0
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 2..98
IPR035892C2 domain superfamilySUPERFAMILY49562C2 domain (Calcium/lipid-binding domain, CaLB)coord: 141..263
IPR045050Synaptotagmin, plantPANTHERPTHR10774EXTENDED SYNAPTOTAGMIN-RELATEDcoord: 1..269
NoneNo IPR availablePANTHERPTHR10774:SF193SYNAPTOTAGMIN-5-LIKEcoord: 1..269
NoneNo IPR availableCDDcd00030C2coord: 146..242
e-value: 2.26557E-28
score: 102.53
NoneNo IPR availableCDDcd00030C2coord: 2..69
e-value: 3.61341E-14
score: 64.7807

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G011870.1CmaCh12G011870.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006869 lipid transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016020 membrane
molecular_function GO:0008289 lipid binding
molecular_function GO:0046872 metal ion binding