CmaCh12G011620 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G011620
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionE3 ubiquitin-protein ligase ZNRF3-like isoform X1
LocationCma_Chr12: 9167513 .. 9170599 (-)
RNA-Seq ExpressionCmaCh12G011620
SyntenyCmaCh12G011620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TTCCTTGTTTTCTCTCACTACTTTCATGAGAGAGAGAGAGAGGAATGATTGAGAAAATCGTGTTGTTATAATTGTACGTTTTACGCTCACCTTCAATGGCGGGGACCCGTTTTTGTCCATTACCTTCATCACTTCCATGCTCCGTTGGCCTTCGTTCTTTATCCTTATTTTAATTCATTCTGCTCCAATTTCCCCATCTTCGATCGATCTTTCCAGTTCTTTTCATTTTGTTCTTCGTGTTCTTGATTTTTCTATTTCTGGATTTCTTGTTTGAGTTGCTGGAAATGAGAGATTTCGATTGGAATTGTTCTTTTACAGCGCATCTGGATTTTGGTTCATTTTGATTAAACTGAGAAACTCCATTGATTTTGAGTTGGAATTGTTCTGGATTTGGGGAGTTTTTCTCTGTTCTTTGTCGTTCTGGCTTTTCAGCAACTGCGTGGTTCTGATTCATTTGCTGAAACTGAGAAAATCAGTGAGTGTTCGGGGATTTTATAAGCTATGTTTTCTCTGTTCTTCTTCATCCGATGAACTCCATTGATTCTGAGTTAAAATCAGTGCGTGTTCGCGGATTTTACAAGTTGTCTTCTCTCTGTTCTTCTTCATCTGATGAACTCCATTGATTCTGAGTTGAAATTAGTGAGGGTTTGGGGATTTTATAAGTTATCTTCTCTCTGTTCTTCATCATGTGATGAACTCCATTGATTTCAAGTTGAAATCAGTGAGTATTTGGGGATTTTACAAGTTATCTTTTCTCTGTTCTTCTTCATGTGATGAACTCCATTGATTCCGAGTTGAAATCGGTGAATATTTGGGGATTTTACGAGTTATCTTTTCTCTGTTCTTCTTCATGTGATGAACTCCATTGATTCCGAGTTGAAATCAGTGAGTGTTTGGGGATTTTATAAGTTATCTTCTCTCTGTTCTTCATCATCTGATGAACTCCACTGATTCTGAGTGGAAATCGGTGAGTGTTCGAGGATTTTACAAGTTATCTTCTCTCTGTTTTTCTTCATCTGATGAACTCCATTGTTTTTCTGCAATTATGCTCTCATTAATCTGCTGAAACTTACAAGCTCTTCAAATTTTGAATCGAAATCTGCTCAAAATCGTCTTCGAATTTATTTCATCTAAATAAACTCGTTGATCAATGGCTGGAATGTTGCCTGGAGTTGAATGCGCGAGACGGCGGCGGTTCCACCAGAGCATAACGTCATCGGAATCGGCGTCCACGGCGGCGAAATTAGGATCCACAAGAAGGTCCTCCTTCTGTTTGTATACAAGCAACCGTGATTTTCACCTCACTTCATCTTCTTCACAGGTATCAAAACAAATCTAGAAATTCCATCCATATCCTTCGAATTTTCTGTTTGTTTCCCGTGAAAATCTTGATGAATGATTTCAATTGTATAAGCAACGGTGCTCTTCGAATCACGCTTACCAGAACGAGAAGCTTGGGCAAGCTGCAAGGGAAGCTAAGGAACGGTTGGATGAGAGGTTGAGAACGCACAGAAAACCTGAGAATTCAAGGTAATTTCTTCATGAACCACCTTATTTCTTCATAATTTTGTTTCATCTTTATACATATATATATAATAGGAATTAAAATATTATTTTAGTTGATTTTTGTACTATGAGGAGAAAGAGTGTGGAAACCTTTCACTAGCAAATGTGTTCAACTTCTCCTTACCCGATAGTTATATGCAGTAACCTATTGTTATTGTCTTTTGCTTACATTCCCATATAACACCAATTTCAATTTTTCAAATCTTACTTTCGAAATCTCTCTGCCTCTGTTTTCTAAAACCTTCTTCTTGAATCGAGGCCAAAGGCTCGAGCATACGTCACTTGACAGTAGGCGATCCAAGAACAAATAGACTTAACTCACTTGTTGCTGGAAAGTGAGTGTTACACAATCGCAAGTCTGCTATCATCAAACAACGGCTTACTGCATATAACTATCGGGTAGGGAGATATGACTAGTAAAACCTAAACAAGATAGTATGGGCTAGGTGCAGGGTTAGGGTTAGGTTGTTATATTTAATTTTAGTTTATATAGTTTAATTAGCATATAAATTGTTATTATTCTTATTTAAAAAATTATATTATTTTAAAAATATTTTAATCATATTTGAACTAAAATTAAATAAACTTGAAAGTAAAGAAAATAAAAACTATAAAAATCATTATCGATCATTGGAGCGGGTAGCAATTAGGTCCTTCAACTTTGTTAAGTGTTGAAACTATTATCGTTTACTGTTTTTTTTTGTCTTCTATATTGATTGACAATAAATTGGGGTTTTCGTGTTAACCCATAAGATAATTAATTAAATTAAAAGTTTAAGGATATAATTAAAACCCGAAACAAAGTTGAAGAAGTTGATTGCAATAAGTAACCAAGTTTAGGGATTAATTGGTCAAATGTTTTGTTTTAATTATTAAAAGAAGAAAAAAATAGATTTGAAAAGTTTGAATTTAGTATCTTGACAAATCGTCTCTAAACCATACCTGATAGCCACGTAAAAACCCAATTCACTTTCGTTCATTTTTACTCGACCCTATAATTTTATCCTGACAACTAACGAATTAGTTGCTTGAAAAATTTAATAACACAAGAAGACAACAAGGCTCTACTAAATTATCGCAATAGAGCCAATTCAAAACTTTTAAATCATGTTCAAACTACGAGAATAGCCAAATTTAAAATTAACGTTAAAAATCTATTGATTTTCTTTTTGAATTATAGGACGAAGAGTAGTAGTGAAGAAGTAAGGTTGAAGAAAGAGAATGTGAAGGGAAAGGAAGAAACAACAACCAAGAAGAGGTTCAGTTGGGGGAAGCTAATGAAATGGAAAGCCTCCGAACAAGAAGAGTGCACTGTTTGTCTCGAAAGGTTCAGAGCGGCCGAGCCACTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCCATGTGCTTGGTCCCTTGGCTCCAAGCTAATGCCCATTGCCCTTGTTGTAGGTACCCTATTCTCGCTCAATAACAAACGAGAAAGTATTCGAATAAATTAGATGTACGAGAAACCATTAAAAAATTAAAAGTGATTAAAC

mRNA sequence

TTCCTTGTTTTCTCTCACTACTTTCATGAGAGAGAGAGAGAGGAATGATTGAGAAAATCGTGTTGTTATAATTGTACGTTTTACGCTCACCTTCAATGGCGGGGACCCGTTTTTGTCCATTACCTTCATCACTTCCATGCTCCGTTGGCCTTCGTTCTTTATCCTTATTTTAATTCATTCTGCTCCAATTTCCCCATCTTCGATCGATCTTTCCAGTTCTTTTCATTTTGTTCTTCGTGTTCTTGATTTTTCTATTTCTGGATTTCTTGTTTGAGTTGCTGGAAATGAGAGATTTCGATTGGAATTGTTCTTTTACAGCGCATCTGGATTTTGGTTCATTTTGATTAAACTGAGAAACTCCATTGATTTTGAGTTGGAATTGTTCTGGATTTGGGGAGTTTTTCTCTGTTCTTTGTCGTTCTGGCTTTTCAGCAACTGCGTGGTTCTGATTCATTTGCTGAAACTGAGAAAATCAGTGAGTGTTCGGGGATTTTATAAGCTATGTTTTCTCTGTTCTTCTTCATCCGATGAACTCCATTGATTCTGAGTTAAAATCAGTGCGTGTTCGCGGATTTTACAAGTTGTCTTCTCTCTGTTCTTCTTCATCTGATGAACTCCATTGATTCTGAGTTGAAATTAGTGAGGGTTTGGGGATTTTATAAGTTATCTTCTCTCTGTTCTTCATCATGTGATGAACTCCATTGATTTCAAGTTGAAATCAGTGAGTATTTGGGGATTTTACAAGTTATCTTTTCTCTGTTCTTCTTCATGTGATGAACTCCATTGATTCCGAGTTGAAATCGGTGAATATTTGGGGATTTTACGAGTTATCTTTTCTCTGTTCTTCTTCATGTGATGAACTCCATTGATTCCGAGTTGAAATCAGTGAGTGTTTGGGGATTTTATAAGTTATCTTCTCTCTGTTCTTCATCATCTGATGAACTCCACTGATTCTGAGTGGAAATCGGTGAGTGTTCGAGGATTTTACAAGTTATCTTCTCTCTGTTTTTCTTCATCTGATGAACTCCATTGTTTTTCTGCAATTATGCTCTCATTAATCTGCTGAAACTTACAAGCTCTTCAAATTTTGAATCGAAATCTGCTCAAAATCGTCTTCGAATTTATTTCATCTAAATAAACTCGTTGATCAATGGCTGGAATGTTGCCTGGAGTTGAATGCGCGAGACGGCGGCGGTTCCACCAGAGCATAACGTCATCGGAATCGGCGTCCACGGCGGCGAAATTAGGATCCACAAGAAGGTCCTCCTTCTGTTTGTATACAAGCAACCGTGATTTTCACCTCACTTCATCTTCTTCACAGCAACGGTGCTCTTCGAATCACGCTTACCAGAACGAGAAGCTTGGGCAAGCTGCAAGGGAAGCTAAGGAACGGTTGGATGAGAGGTTGAGAACGCACAGAAAACCTGAGAATTCAAGGACGAAGAGTAGTAGTGAAGAAGTAAGGTTGAAGAAAGAGAATGTGAAGGGAAAGGAAGAAACAACAACCAAGAAGAGGTTCAGTTGGGGGAAGCTAATGAAATGGAAAGCCTCCGAACAAGAAGAGTGCACTGTTTGTCTCGAAAGGTTCAGAGCGGCCGAGCCACTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCCATGTGCTTGGTCCCTTGGCTCCAAGCTAATGCCCATTGCCCTTGTTGTAGGTACCCTATTCTCGCTCAATAACAAACGAGAAAGTATTCGAATAAATTAGATGTACGAGAAACCATTAAAAAATTAAAAGTGATTAAAC

Coding sequence (CDS)

ATGGCTGGAATGTTGCCTGGAGTTGAATGCGCGAGACGGCGGCGGTTCCACCAGAGCATAACGTCATCGGAATCGGCGTCCACGGCGGCGAAATTAGGATCCACAAGAAGGTCCTCCTTCTGTTTGTATACAAGCAACCGTGATTTTCACCTCACTTCATCTTCTTCACAGCAACGGTGCTCTTCGAATCACGCTTACCAGAACGAGAAGCTTGGGCAAGCTGCAAGGGAAGCTAAGGAACGGTTGGATGAGAGGTTGAGAACGCACAGAAAACCTGAGAATTCAAGGACGAAGAGTAGTAGTGAAGAAGTAAGGTTGAAGAAAGAGAATGTGAAGGGAAAGGAAGAAACAACAACCAAGAAGAGGTTCAGTTGGGGGAAGCTAATGAAATGGAAAGCCTCCGAACAAGAAGAGTGCACTGTTTGTCTCGAAAGGTTCAGAGCGGCCGAGCCACTGGTCCATCTTCCATGCGCCCACAAGTTCCACTCCATGTGCTTGGTCCCTTGGCTCCAAGCTAATGCCCATTGCCCTTGTTGTAGGTACCCTATTCTCGCTCAATAA

Protein sequence

MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRCSSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCRYPILAQ
Homology
[Close]
BLAST of CmaCh12G011620 vs. ExPASy Swiss-Prot
Match: Q852U6 (Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RHY1A PE=1 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 5.1e-11
Identity = 27/50 (54.00%), Postives = 33/50 (66.00%), Query Frame = 0 

Query: 134 SEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCRYPI 184
           SE  +C++CLE F   + L+ LPC H FHS CL PWL+A   CPCCR  I
Sbjct: 198 SEMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCRRAI 247

[Close]
BLAST of CmaCh12G011620 vs. ExPASy Swiss-Prot
Match: Q9C1X4 (Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=SPAP32A8.03c PE=4 SV=1)

HSP 1 Score: 63.5 bits (153), Expect = 2.8e-09
Identity = 22/49 (44.90%), Postives = 32/49 (65.31%), Query Frame = 0 

Query: 135 EQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCRYPI 184
           E+ ECT+C+E F+  + ++ LPC H FH  C+ PWL+ N  C  CR P+
Sbjct: 392 EEGECTICMEMFKINDDVIQLPCKHYFHENCIKPWLRVNGTCAICRAPV 440

[Close]
BLAST of CmaCh12G011620 vs. ExPASy Swiss-Prot
Match: Q08D68 (E3 ubiquitin-protein ligase ZNRF3 OS=Xenopus tropicalis OX=8364 GN=znrf3 PE=1 SV=1)

HSP 1 Score: 58.2 bits (139), Expect = 1.2e-07
Identity = 33/97 (34.02%), Postives = 51/97 (52.58%), Query Frame = 0 

Query: 90  RKPENSRTKSSSEEVRLKKENVKGKEETTTKKRFSWGKLMKWKASEQEECTVCLERFRAA 149
           R+ +NS  + + + +  K E  K K +    +  S G L    +S   +C +CLE++   
Sbjct: 218 RRSQNSMNRMAVQALE-KMETRKFKAKGKVPREGSCGGLDTLSSSSTSDCAICLEKYIDG 277

Query: 150 EPLVHLPCAHKFHSMCLVPWLQANAHCPCCRYPILAQ 187
           E L  +PC H+FH  C+ PWL  N  CP CR+ I+ Q
Sbjct: 278 EELRVIPCTHRFHKRCVDPWLLQNHTCPHCRHNIIEQ 313

[Close]
BLAST of CmaCh12G011620 vs. ExPASy Swiss-Prot
Match: Q4KLR8 (E3 ubiquitin-protein ligase ZNRF3 OS=Xenopus laevis OX=8355 GN=znrf3 PE=2 SV=1)

HSP 1 Score: 57.8 bits (138), Expect = 1.5e-07
Identity = 33/97 (34.02%), Postives = 51/97 (52.58%), Query Frame = 0 

Query: 90  RKPENSRTKSSSEEVRLKKENVKGKEETTTKKRFSWGKLMKWKASEQEECTVCLERFRAA 149
           R+ +NS  + + + +  K E  K K +    +  S G L    +S   +C +CLE++   
Sbjct: 154 RRSQNSMNRMAVQALE-KMETRKFKAKGKVSREGSCGGLDTLSSSSISDCAICLEKYIDG 213

Query: 150 EPLVHLPCAHKFHSMCLVPWLQANAHCPCCRYPILAQ 187
           E L  +PC H+FH  C+ PWL  N  CP CR+ I+ Q
Sbjct: 214 EELRVIPCTHRFHKRCVDPWLLQNHTCPHCRHNIIEQ 249

[Close]
BLAST of CmaCh12G011620 vs. ExPASy Swiss-Prot
Match: A5WWA0 (E3 ubiquitin-protein ligase znrf3 OS=Danio rerio OX=7955 GN=znrf3 PE=1 SV=2)

HSP 1 Score: 55.8 bits (133), Expect = 5.9e-07
Identity = 33/100 (33.00%), Postives = 52/100 (52.00%), Query Frame = 0 

Query: 91  KPENSRTKSSSEEVRL----KKENVKGKEETTTKKRFSWGKLMKWKASEQEECTVCLERF 150
           K +  R++SS   + +    K E  K K +   ++  S G      +S   +C +CLE++
Sbjct: 215 KLKQRRSQSSMNRMAIQALEKMETCKFKAKFKGQREASCGASDSVSSSSTSDCAICLEKY 274

Query: 151 RAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCRYPILAQ 187
              E L  +PCAH+FH  C+ PWL  +  CP CR+ I+ Q
Sbjct: 275 IDGEELRVIPCAHRFHKKCVDPWLLQHHTCPHCRHNIIDQ 314

[Close]
BLAST of CmaCh12G011620 vs. ExPASy TrEMBL
Match: A0A6J1HQI5 (probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111465688 PE=4 SV=1)

HSP 1 Score: 366.7 bits (940), Expect = 5.8e-98
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60
           MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60

Query: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120
           SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK
Sbjct: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120

Query: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180
           KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR
Sbjct: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180

Query: 181 YPILAQ 187
           YPILAQ
Sbjct: 181 YPILAQ 186

[Close]
BLAST of CmaCh12G011620 vs. ExPASy TrEMBL
Match: A0A6J1FHS8 (E3 ubiquitin-protein ligase ZNRF3-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111444265 PE=4 SV=1)

HSP 1 Score: 344.0 bits (881), Expect = 4.0e-91
Identity = 174/186 (93.55%), Postives = 178/186 (95.70%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60
           MAGMLPGVECARRRRFHQSITSS+SASTAAKLGSTRRSSFCLY SNRDFHLTSSSSQQRC
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYASNRDFHLTSSSSQQRC 60

Query: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120
           SSNHAYQ+EKLGQAAREAKERLDERLRTHRKPENSR KSSSEEV LKKE  KGKEETTTK
Sbjct: 61  SSNHAYQDEKLGQAAREAKERLDERLRTHRKPENSRKKSSSEEVGLKKEKAKGKEETTTK 120

Query: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180
           KRF WGKLMKWKASEQEECTVCLE+FRA EPLVHLPCAHKFHS CLVPWLQANA+CPCCR
Sbjct: 121 KRFGWGKLMKWKASEQEECTVCLEKFRATEPLVHLPCAHKFHSACLVPWLQANAYCPCCR 180

Query: 181 YPILAQ 187
           YPILAQ
Sbjct: 181 YPILAQ 186

[Close]
BLAST of CmaCh12G011620 vs. ExPASy TrEMBL
Match: A0A6J1HSD8 (probable E3 ubiquitin-protein ligase RHY1A isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111465688 PE=4 SV=1)

HSP 1 Score: 338.2 bits (866), Expect = 2.2e-89
Identity = 176/186 (94.62%), Postives = 176/186 (94.62%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60
           MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSS    
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSS---- 60

Query: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120
                 QNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK
Sbjct: 61  ------QNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120

Query: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180
           KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR
Sbjct: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 176

Query: 181 YPILAQ 187
           YPILAQ
Sbjct: 181 YPILAQ 176

[Close]
BLAST of CmaCh12G011620 vs. ExPASy TrEMBL
Match: A0A6J1FC43 (probable E3 ubiquitin-protein ligase RHY1A isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111444265 PE=4 SV=1)

HSP 1 Score: 315.5 bits (807), Expect = 1.5e-82
Identity = 164/186 (88.17%), Postives = 168/186 (90.32%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60
           MAGMLPGVECARRRRFHQSITSS+SASTAAKLGSTRRSSFCLY SNRDFHLTSSSS    
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYASNRDFHLTSSSS---- 60

Query: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120
                 Q+EKLGQAAREAKERLDERLRTHRKPENSR KSSSEEV LKKE  KGKEETTTK
Sbjct: 61  ------QDEKLGQAAREAKERLDERLRTHRKPENSRKKSSSEEVGLKKEKAKGKEETTTK 120

Query: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180
           KRF WGKLMKWKASEQEECTVCLE+FRA EPLVHLPCAHKFHS CLVPWLQANA+CPCCR
Sbjct: 121 KRFGWGKLMKWKASEQEECTVCLEKFRATEPLVHLPCAHKFHSACLVPWLQANAYCPCCR 176

Query: 181 YPILAQ 187
           YPILAQ
Sbjct: 181 YPILAQ 176

[Close]
BLAST of CmaCh12G011620 vs. ExPASy TrEMBL
Match: A0A6J1G4D6 (probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC111450678 PE=4 SV=1)

HSP 1 Score: 281.6 bits (719), Expect = 2.4e-72
Identity = 147/184 (79.89%), Postives = 155/184 (84.24%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSS--SQQ 60
           MAGMLPGVECARRRRFHQSITSS++ STAAKLGSTRR SFCLYTSNRDFH+TSSS  SQQ
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDTPSTAAKLGSTRRPSFCLYTSNRDFHITSSSSCSQQ 60

Query: 61  RCSSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETT 120
           R SS+ AYQNEKLG+ AREAKERLDERLRTHRKPEN R          KK     KEE+ 
Sbjct: 61  RSSSHQAYQNEKLGEIAREAKERLDERLRTHRKPENPR----------KKSGEGWKEESI 120

Query: 121 TKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPC 180
           TKKRF+WGKLMKWKASEQEEC VCLE FRA EPLVHLPCAHKFHS CLVPWLQ NAHCPC
Sbjct: 121 TKKRFTWGKLMKWKASEQEECAVCLEGFRAGEPLVHLPCAHKFHSACLVPWLQTNAHCPC 174

Query: 181 CRYP 183
           CR+P
Sbjct: 181 CRFP 174

[Close]
BLAST of CmaCh12G011620 vs. NCBI nr
Match: XP_022965966.1 (probable E3 ubiquitin-protein ligase RHY1A isoform X1 [Cucurbita maxima] >XP_022965967.1 probable E3 ubiquitin-protein ligase RHY1A isoform X1 [Cucurbita maxima] >XP_022965968.1 probable E3 ubiquitin-protein ligase RHY1A isoform X1 [Cucurbita maxima])

HSP 1 Score: 366.7 bits (940), Expect = 1.2e-97
Identity = 186/186 (100.00%), Postives = 186/186 (100.00%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60
           MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60

Query: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120
           SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK
Sbjct: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120

Query: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180
           KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR
Sbjct: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180

Query: 181 YPILAQ 187
           YPILAQ
Sbjct: 181 YPILAQ 186

[Close]
BLAST of CmaCh12G011620 vs. NCBI nr
Match: XP_023538036.1 (probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo])

HSP 1 Score: 352.8 bits (904), Expect = 1.8e-93
Identity = 179/186 (96.24%), Postives = 180/186 (96.77%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60
           MAGMLPGVECARRRRFHQSITSS+SASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60

Query: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120
           SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSR KSSSEEV LKKE  KGKEETTTK
Sbjct: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRKKSSSEEVGLKKEKAKGKEETTTK 120

Query: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180
           KRFSWGKLMKWKASEQEECTVCLERFRA EPLVHLPCAHKFHS CLVPWLQANAHCPCCR
Sbjct: 121 KRFSWGKLMKWKASEQEECTVCLERFRAVEPLVHLPCAHKFHSTCLVPWLQANAHCPCCR 180

Query: 181 YPILAQ 187
           YPILAQ
Sbjct: 181 YPILAQ 186

[Close]
BLAST of CmaCh12G011620 vs. NCBI nr
Match: XP_022938062.1 (E3 ubiquitin-protein ligase ZNRF3-like isoform X1 [Cucurbita moschata] >XP_022938063.1 E3 ubiquitin-protein ligase ZNRF3-like isoform X1 [Cucurbita moschata] >XP_022938064.1 E3 ubiquitin-protein ligase ZNRF3-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 344.0 bits (881), Expect = 8.2e-91
Identity = 174/186 (93.55%), Postives = 178/186 (95.70%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60
           MAGMLPGVECARRRRFHQSITSS+SASTAAKLGSTRRSSFCLY SNRDFHLTSSSSQQRC
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYASNRDFHLTSSSSQQRC 60

Query: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120
           SSNHAYQ+EKLGQAAREAKERLDERLRTHRKPENSR KSSSEEV LKKE  KGKEETTTK
Sbjct: 61  SSNHAYQDEKLGQAAREAKERLDERLRTHRKPENSRKKSSSEEVGLKKEKAKGKEETTTK 120

Query: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180
           KRF WGKLMKWKASEQEECTVCLE+FRA EPLVHLPCAHKFHS CLVPWLQANA+CPCCR
Sbjct: 121 KRFGWGKLMKWKASEQEECTVCLEKFRATEPLVHLPCAHKFHSACLVPWLQANAYCPCCR 180

Query: 181 YPILAQ 187
           YPILAQ
Sbjct: 181 YPILAQ 186

[Close]
BLAST of CmaCh12G011620 vs. NCBI nr
Match: XP_022965969.1 (probable E3 ubiquitin-protein ligase RHY1A isoform X2 [Cucurbita maxima])

HSP 1 Score: 338.2 bits (866), Expect = 4.5e-89
Identity = 176/186 (94.62%), Postives = 176/186 (94.62%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60
           MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSS    
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSS---- 60

Query: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120
                 QNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK
Sbjct: 61  ------QNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120

Query: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180
           KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR
Sbjct: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 176

Query: 181 YPILAQ 187
           YPILAQ
Sbjct: 181 YPILAQ 176

[Close]
BLAST of CmaCh12G011620 vs. NCBI nr
Match: KAG6586351.1 (putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 317.0 bits (811), Expect = 1.1e-82
Identity = 164/186 (88.17%), Postives = 168/186 (90.32%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFHQSITSSESASTAAKLGSTRRSSFCLYTSNRDFHLTSSSSQQRC 60
           MAGMLPGVECARRRRFHQSITSS+SASTAAKLGSTRRSSFCLY SNRDFHLTSSSS    
Sbjct: 1   MAGMLPGVECARRRRFHQSITSSDSASTAAKLGSTRRSSFCLYASNRDFHLTSSSS---- 60

Query: 61  SSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVKGKEETTTK 120
                 QNEKLGQAAREAKERLDERLRTHRKPENSR KSSSEEV LKKE  KGKEETTTK
Sbjct: 61  ------QNEKLGQAAREAKERLDERLRTHRKPENSRKKSSSEEVGLKKEKAKGKEETTTK 120

Query: 121 KRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCR 180
           KRF WGKLMKWKASEQEECTVCLE+FRAAEPLVHLPCAHKFHS CL+PWLQANA CPCCR
Sbjct: 121 KRFGWGKLMKWKASEQEECTVCLEKFRAAEPLVHLPCAHKFHSTCLIPWLQANADCPCCR 176

Query: 181 YPILAQ 187
           YP+LAQ
Sbjct: 181 YPVLAQ 176

[Close]
BLAST of CmaCh12G011620 vs. TAIR 10
Match: AT2G15580.1 (RING/U-box superfamily protein )

HSP 1 Score: 147.9 bits (372), Expect = 8.1e-36
Identity = 98/201 (48.76%), Postives = 123/201 (61.19%), Query Frame = 0 

Query: 1   MAGMLPGVECARRRRFH---QSITSSESASTAAKLGS--TRRSSFCLYTSNRDFHLTSSS 60
           MAGMLPGVECARRRRFH     I SS +AS AA  G   TRR SF LYT+N + H    S
Sbjct: 1   MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61  SQQRCSSNHAY---QNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENVK 120
             +R   N +Y    +EKL  AA+EAK+RL++RLR        RT+ + ++   K E  K
Sbjct: 61  FSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLRI-----PPRTRQNGKDKGNKLEQGK 120

Query: 121 GK-------EETTTKKRFSWGKLMKW---KASEQEECTVCLERFRAAEPLVHLPCAHKFH 180
           GK       E    KK  S G+LM+W   +  EQ++C +CL+RF+  E LVHLPCAHKFH
Sbjct: 121 GKPLGDLPTEVVGLKK--SRGRLMEWFKRRVREQQDCAICLDRFKKGETLVHLPCAHKFH 180

Query: 181 SMCLVPWLQANAHCPCCRYPI 184
           S+CL+PWL  N +CP CR  I
Sbjct: 181 SICLLPWLDTNVYCPYCRTDI 194

[Close]
BLAST of CmaCh12G011620 vs. TAIR 10
Match: AT2G15580.2 (RING/U-box superfamily protein )

HSP 1 Score: 75.5 bits (184), Expect = 5.1e-14
Identity = 52/95 (54.74%), Postives = 62/95 (65.26%), Query Frame = 0 

Query: 1  MAGMLPGVECARRRRFH---QSITSSESASTAAKLGS--TRRSSFCLYTSNRDFHLTSSS 60
          MAGMLPGVECARRRRFH     I SS +AS AA  G   TRR SF LYT+N + H    S
Sbjct: 1  MAGMLPGVECARRRRFHGGAPPIESSNTASVAAAAGHVWTRRPSFSLYTTNHESHQAHVS 60

Query: 61 SQQRCSSNHAY---QNEKLGQAAREAKERLDERLR 88
            +R   N +Y    +EKL  AA+EAK+RL++RLR
Sbjct: 61 FSERSVRNKSYGEDNDEKLDGAAKEAKQRLNKRLR 95

[Close]
BLAST of CmaCh12G011620 vs. TAIR 10
Match: AT1G49850.1 (RING/U-box superfamily protein )

HSP 1 Score: 69.3 bits (168), Expect = 3.7e-12
Identity = 27/50 (54.00%), Postives = 33/50 (66.00%), Query Frame = 0 

Query: 134 SEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQANAHCPCCRYPI 184
           SE  +C++CLE F   + L+ LPC H FHS CL PWL+A   CPCCR  I
Sbjct: 198 SEMRDCSICLESFTKGDMLISLPCTHSFHSSCLNPWLRACGDCPCCRRAI 247

[Close]
BLAST of CmaCh12G011620 vs. TAIR 10
Match: AT1G55530.1 (RING/U-box superfamily protein )

HSP 1 Score: 61.2 bits (147), Expect = 9.9e-10
Identity = 30/77 (38.96%), Postives = 42/77 (54.55%), Query Frame = 0 

Query: 109 ENVKGKEETTTKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVP 168
           EN   +  T   K+ +   L   K  E  +C+VCL+ F        +PC HKFHS CL+P
Sbjct: 193 ENDPNRYGTPPAKKEAVEALATVKIEETLQCSVCLDDFEIGTEAKLMPCTHKFHSDCLLP 252

Query: 169 WLQANAHCPCCRYPILA 186
           WL+ ++ CP CRY + A
Sbjct: 253 WLELHSSCPVCRYQLPA 269

[Close]
BLAST of CmaCh12G011620 vs. TAIR 10
Match: AT3G58720.1 (RING/U-box superfamily protein )

HSP 1 Score: 50.4 bits (119), Expect = 1.8e-06
Identity = 37/133 (27.82%), Postives = 54/133 (40.60%), Query Frame = 0 

Query: 52  TSSSSQQRCSSNHAYQNEKLGQAAREAKERLDERLRTHRKPENSRTKSSSEEVRLKKENV 111
           T   S  R SS    ++E L    +E    + +           R K+   E     +N+
Sbjct: 76  TRPESSSRSSSQTNEEDEILKHLTKETYNPVPKSTLLRSLSLYYRNKNPGSESSRNTQNL 135

Query: 112 KGKEETTTKKRFSWGKLMKWKASEQEECTVCLERFRAAEPLVHLPCAHKFHSMCLVPWLQ 171
            G+E                   + + C+VCLE F   E ++  PC H FH  C+VPWL+
Sbjct: 136 SGEE-------------------DDKRCSVCLEDFEPKETVMLTPCKHMFHEECIVPWLK 189

Query: 172 ANAHCPCCRYPIL 185
               CP CR+ IL
Sbjct: 196 TKGQCPVCRFVIL 189

The following BLAST results are available for this feature:
BLAST of CmaCh12G011620 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
Q852U65.1e-1154.00Probable E3 ubiquitin-protein ligase RHY1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
Q9C1X42.8e-0944.90Uncharacterized RING finger protein P32A8.03c OS=Schizosaccharomyces pombe (stra... [more]
Q08D681.2e-0734.02E3 ubiquitin-protein ligase ZNRF3 OS=Xenopus tropicalis OX=8364 GN=znrf3 PE=1 SV... [more]
Q4KLR81.5e-0734.02E3 ubiquitin-protein ligase ZNRF3 OS=Xenopus laevis OX=8355 GN=znrf3 PE=2 SV=1[more]
A5WWA05.9e-0733.00E3 ubiquitin-protein ligase znrf3 OS=Danio rerio OX=7955 GN=znrf3 PE=1 SV=2[more]
BLAST of CmaCh12G011620 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1HQI55.8e-98100.00probable E3 ubiquitin-protein ligase RHY1A isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1FHS84.0e-9193.55E3 ubiquitin-protein ligase ZNRF3-like isoform X1 OS=Cucurbita moschata OX=3662 ... [more]
A0A6J1HSD82.2e-8994.62probable E3 ubiquitin-protein ligase RHY1A isoform X2 OS=Cucurbita maxima OX=366... [more]
A0A6J1FC431.5e-8288.17probable E3 ubiquitin-protein ligase RHY1A isoform X2 OS=Cucurbita moschata OX=3... [more]
A0A6J1G4D62.4e-7279.89probable E3 ubiquitin-protein ligase RHY1A OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
BLAST of CmaCh12G011620 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022965966.11.2e-97100.00probable E3 ubiquitin-protein ligase RHY1A isoform X1 [Cucurbita maxima] >XP_022... [more]
XP_023538036.11.8e-9396.24probable E3 ubiquitin-protein ligase RHY1A [Cucurbita pepo subsp. pepo][more]
XP_022938062.18.2e-9193.55E3 ubiquitin-protein ligase ZNRF3-like isoform X1 [Cucurbita moschata] >XP_02293... [more]
XP_022965969.14.5e-8994.62probable E3 ubiquitin-protein ligase RHY1A isoform X2 [Cucurbita maxima][more]
KAG6586351.11.1e-8288.17putative E3 ubiquitin-protein ligase RHY1A, partial [Cucurbita argyrosperma subs... [more]
BLAST of CmaCh12G011620 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G15580.18.1e-3648.76RING/U-box superfamily protein [more]
AT2G15580.25.1e-1454.74RING/U-box superfamily protein [more]
AT1G49850.13.7e-1254.00RING/U-box superfamily protein [more]
AT1G55530.19.9e-1038.96RING/U-box superfamily protein [more]
AT3G58720.11.8e-0627.82RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 139..179
e-value: 1.3E-4
score: 31.3
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 138..180
e-value: 1.3E-10
score: 41.5
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 139..180
score: 12.474524
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 105..185
e-value: 1.4E-17
score: 64.6
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 69..117
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 73..117
NoneNo IPR availablePANTHERPTHR22765RING FINGER AND PROTEASE ASSOCIATED DOMAIN-CONTAININGcoord: 1..181
NoneNo IPR availablePANTHERPTHR22765:SF204RING/U-BOX SUPERFAMILY PROTEINcoord: 1..181
NoneNo IPR availableCDDcd16454RING-H2_PA-TM-RINGcoord: 138..180
e-value: 3.6809E-16
score: 66.9417
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 126..185

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G011620.1CmaCh12G011620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
molecular_function GO:0061630 ubiquitin protein ligase activity