CmaCh12G011320 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G011320
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionprotein WVD2-like 1
LocationCma_Chr12: 8920977 .. 8924282 (-)
RNA-Seq ExpressionCmaCh12G011320
SyntenyCmaCh12G011320
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GTTTTTTGTTTTCCGATCATTACGCTAACAAAAAGGAAAACCGCTAGCTTTGATTTGTAGAAAGGAAAGAAAGAAAAACCCAATTTTCAATTTATTTTCCCACTTTGTTTTATTTTCTTTTGTTTTCGTTTGGTTTTAGAGAGCGGGGAGGCGAGGTTAGAGGGGCGGGGAGCCAGGGCGATCTTGTCGGATCGGCAAGAGGAGCTGGTTTCTCAGGGTACCTTCTTCTTCTCCTCTGTTTTTCATGCCTTTTCTGCTTTCTTCCTTCCATTCCTCTGTTTTTCTTTTCATCTTCTTTGCTGTAATGGGTTGAATATGGCTCTTCTGCTGTTCTTAATGCTGTGTTTTGAGCTGTTTCTGCTGTTTTTTGGTTCTTGTTTGATGTCTTGTGGTGTTTTGAGGCTTTGTGGAAATGGGGTTTTCTTGTTTTTTGAATCCTTGCATGTTTGTTTGTGGGAGTTCTTGTTTGTGGAGTTGGGAATCTGGATTAACCCTTTTGATTTTATGGGTCAATTCAAATATATTTGAAGTGTTCTTGAGGATTGATTTCATTTTGATGTTGTTCTTTCCTTTATGATCTTGTTTTTCATGGTGTTTTGGGGCAAACCATGGATTTTCTGAGTTGGATCTTTCTAATATAGCTGACACATTTTAAAAACCGTGAGGGAAAGCCCAAAGAGGACAATATAGCGGTAGGCTTGGGCTGTTACAAATGGTATCACAATCAGGCATAGGGTGATGTGCTAGCGTAGGCTGAGCCCTAAAGGGGGTGGACACGAGGCGATGTGCAGCAAGGATGCTGGGTATGGAAGGGGGGGTGGATTGGAGAGGTCCACATTGATTGGAGAAGGGAACGAGTGCCAACGAGGACGCTGGACCCTGAAGGGGGGTGGATTGTGAAATCCCACATCGGTTGGGGAAGAGAACGAAAGACTCTTTATAAGGGTGTGGAAATCTCTGTTCTAAAAATCTTGAGGGAAAGCCCAAAAAGGACAATATATGCTAGCGGTGGGTTTGGGCTGTTACAAATGGTATCACAGCCAGACACATGGTAATGTGCCAAGGCTGAGCCTTGAAGAGGGTGGACATGAACCGGTGTGCTAGCAAGGACGCTGGGCTCCAAAATGGGTGGATTGGGAAGGTCCCACATCGATTGCAGAAGAGAACGAGTGCCAACGAGGATGCTGTACCCCGAAGGGGGTGGATTGTGAAATCCCACATCGGTTGGGGAAGAGGACGAAATACCCTTTATAAGGGTGTGGAAATCTCTTCCTAACAGACCCGTTTTAAAAACCTTGAGGGAAAGCCCAAAGAAGATAATATGTGCGCTGGCGGTGGACTTGGACTGATACCATGTTCATTTTTTTGTAGGGTCATGGGTAGGGAAATTGCTTGTGTTGTGGAGGATCCAAATGATCTTGAAATGGTTTTAAATGGTGTTTCTCATGACAAAGTTCATGATTCCTCCAAAACTTCAGAAGAAAGTATTGATTTGGAGGAATTCGAGGATAATGAATCGAGTGAGGAGAATTCGTTAGATGAAAATCGTCAAGAAACGCTGGACGGTGTTCTAGCCATCAAAACCACAATCATTGATGCTAGTGTGCCTATACCTGTCCCTTTGCTTGCTCCCGCATCCGAGCTTGAACCTAAACCTGAACCTGAGCCTGAGCCTGAACTTGTGCCCGTGCCCCTGCTTGTTCCTGAGGAGAAGAAGAAGGAGGAGCGAACTGTTGCACAGAAAATCAGTGACTTCAATAAGTCTATCTCGCCTGGGACTATAGCTGTTACTCCGAAAATCGTGCGGGTGAACCACAAGATTCAACAACCAACTGTTCAGGTACAGAGAAAAGGAGCAATGTACGCACAAACCATTGAGACCGAACGTGCTCCAACTCCAACAACCGTCGTCGATGTTTCGTCGAATGCCATCACATCAAACTCGAAGTCTCCCGGCCCAAAAGAGAATTCATTAGTAAGTACAACTTTTAGGAAGATCAATGGAGCTGCATTTTTTCCTATATATATCCTTAAATACAAGATTATCGACTCGAAAACAGCTTCCCGACTTTGATTTTTGAGTTCAAACAAGTATTATGTGCTGATTTCTGCGTGTGTGTTTTACTGTCTACAGCCAAACTCGCCGCGACCATCATGGAAATCTTCACAGAACGATCTTAAGAAAAATCACGAAGAACAGCCAAACTCGCCTCAAACATCATCGAAATCTTCACATATTTATCTTAAGAAACATCATGGAGAAGAAGAACATTGGTCTACCGCTGCCTCGTATCCTTTTTTTCGACAACGATCTCACAGAATCATGTTTTTGTTCGGTTTCATAAACTATAATTCATTCATATGATCAGATTTTGTTATCCTTAGCCGATTGACAGTACCTTCGCATCAATTCGACAGCTCAAATCTAAAGTTACTATCGGAACAGCTCCCACATTTCAATCAGCCAAACGTGCTGATAAAAGGAAGGAGGTACGTCGGTCGTATGATGGAGACATGTTCTTGCTGTTCTATCTGGGATTTTCTGTTTATATATCAAATGTTTTTTCTCTGTTCAGTACTACAACAAGTTGGAGGAGAAACACAAAGCTTTGGAGGCCGAGAGAATCGAATACGAAGCACGAATTAAGGTATTCCCTGATACCCATCTTGTCGTGTCGTGTCGTGTAATCACCAAACGATCGTCTTAGTTTCGTCTTCGATTTTAGGAAGAGCAACAAGCAGCAATCAAAGAGCTTAGGAAGGGATTAGTGATCAAAGCTAAACCAGTGCCTAGTTTTTATTATGAAGGACCACCACCTAAGGTTGAACTCAAAAAGGTTGGTCGTCGAAACTCTATACCGATATGATAACGATATGTTTTCAGTGTCGGTAAAATTAGTATGTTTCTTTGTTTCGCGATGATGGTAAGATTTTAACTAGGATCTTATGTCAGTTGCCATTAACTCGACCAAAGTCTCCAAATCTAACTCGAAGGAAAAGCTGCGGTGATGCGACGATGAGCATAGGCCTAGAGGAGAAGGGAAGAGGATGCGCACGAGCGCAGCGCCTTAGCTACGGTAGTCACAAATTTGAATCAGCTAACGGCATACCGTGCAGAAATAAAGCGCAAGCCAATGGGCATTGCTACAGTAATGAAAATTTTAAACATCGAAACCACGTGAAGCAGCATGATAAAGAGACGAGAAAAACGACTCTTGCAACGACCGATCCGCTCAAAAGTAATGTATGTATCCCGATTCACTCATGAACATCTACTTTTTATCTAATGTGAGCTGAAAAAAAAAA

mRNA sequence

GTTTTTTGTTTTCCGATCATTACGCTAACAAAAAGGAAAACCGCTAGCTTTGATTTGTAGAAAGGAAAGAAAGAAAAACCCAATTTTCAATTTATTTTCCCACTTTGTTTTATTTTCTTTTGTTTTCGTTTGGTTTTAGAGAGCGGGGAGGCGAGGTTAGAGGGGCGGGGAGCCAGGGCGATCTTGTCGGATCGGCAAGAGGAGCTGGTTTCTCAGGGGTCATGGGTAGGGAAATTGCTTGTGTTGTGGAGGATCCAAATGATCTTGAAATGGTTTTAAATGGTGTTTCTCATGACAAAGTTCATGATTCCTCCAAAACTTCAGAAGAAAGTATTGATTTGGAGGAATTCGAGGATAATGAATCGAGTGAGGAGAATTCGTTAGATGAAAATCGTCAAGAAACGCTGGACGGTGTTCTAGCCATCAAAACCACAATCATTGATGCTAGTGTGCCTATACCTGTCCCTTTGCTTGCTCCCGCATCCGAGCTTGAACCTAAACCTGAACCTGAGCCTGAGCCTGAACTTGTGCCCGTGCCCCTGCTTGTTCCTGAGGAGAAGAAGAAGGAGGAGCGAACTGTTGCACAGAAAATCAGTGACTTCAATAAGTCTATCTCGCCTGGGACTATAGCTGTTACTCCGAAAATCGTGCGGGTGAACCACAAGATTCAACAACCAACTGTTCAGGTACAGAGAAAAGGAGCAATGTACGCACAAACCATTGAGACCGAACGTGCTCCAACTCCAACAACCGTCGTCGATGTTTCGTCGAATGCCATCACATCAAACTCGAAGTCTCCCGGCCCAAAAGAGAATTCATTACCAAACTCGCCGCGACCATCATGGAAATCTTCACAGAACGATCTTAAGAAAAATCACGAAGAACAGCCAAACTCGCCTCAAACATCATCGAAATCTTCACATATTTATCTTAAGAAACATCATGGAGAAGAAGAACATTGGTCTACCGCTGCCTCTACCTTCGCATCAATTCGACAGCTCAAATCTAAAGTTACTATCGGAACAGCTCCCACATTTCAATCAGCCAAACGTGCTGATAAAAGGAAGGAGTACTACAACAAGTTGGAGGAGAAACACAAAGCTTTGGAGGCCGAGAGAATCGAATACGAAGCACGAATTAAGGAAGAGCAACAAGCAGCAATCAAAGAGCTTAGGAAGGGATTAGTGATCAAAGCTAAACCAGTGCCTAGTTTTTATTATGAAGGACCACCACCTAAGGTTGAACTCAAAAAGTTGCCATTAACTCGACCAAAGTCTCCAAATCTAACTCGAAGGAAAAGCTGCGGTGATGCGACGATGAGCATAGGCCTAGAGGAGAAGGGAAGAGGATGCGCACGAGCGCAGCGCCTTAGCTACGGTAGTCACAAATTTGAATCAGCTAACGGCATACCGTGCAGAAATAAAGCGCAAGCCAATGGGCATTGCTACAGTAATGAAAATTTTAAACATCGAAACCACGTGAAGCAGCATGATAAAGAGACGAGAAAAACGACTCTTGCAACGACCGATCCGCTCAAAAGTAATGTATGTATCCCGATTCACTCATGAACATCTACTTTTTATCTAATGTGAGCTGAAAAAAAAAA

Coding sequence (CDS)

ATGGGTAGGGAAATTGCTTGTGTTGTGGAGGATCCAAATGATCTTGAAATGGTTTTAAATGGTGTTTCTCATGACAAAGTTCATGATTCCTCCAAAACTTCAGAAGAAAGTATTGATTTGGAGGAATTCGAGGATAATGAATCGAGTGAGGAGAATTCGTTAGATGAAAATCGTCAAGAAACGCTGGACGGTGTTCTAGCCATCAAAACCACAATCATTGATGCTAGTGTGCCTATACCTGTCCCTTTGCTTGCTCCCGCATCCGAGCTTGAACCTAAACCTGAACCTGAGCCTGAGCCTGAACTTGTGCCCGTGCCCCTGCTTGTTCCTGAGGAGAAGAAGAAGGAGGAGCGAACTGTTGCACAGAAAATCAGTGACTTCAATAAGTCTATCTCGCCTGGGACTATAGCTGTTACTCCGAAAATCGTGCGGGTGAACCACAAGATTCAACAACCAACTGTTCAGGTACAGAGAAAAGGAGCAATGTACGCACAAACCATTGAGACCGAACGTGCTCCAACTCCAACAACCGTCGTCGATGTTTCGTCGAATGCCATCACATCAAACTCGAAGTCTCCCGGCCCAAAAGAGAATTCATTACCAAACTCGCCGCGACCATCATGGAAATCTTCACAGAACGATCTTAAGAAAAATCACGAAGAACAGCCAAACTCGCCTCAAACATCATCGAAATCTTCACATATTTATCTTAAGAAACATCATGGAGAAGAAGAACATTGGTCTACCGCTGCCTCTACCTTCGCATCAATTCGACAGCTCAAATCTAAAGTTACTATCGGAACAGCTCCCACATTTCAATCAGCCAAACGTGCTGATAAAAGGAAGGAGTACTACAACAAGTTGGAGGAGAAACACAAAGCTTTGGAGGCCGAGAGAATCGAATACGAAGCACGAATTAAGGAAGAGCAACAAGCAGCAATCAAAGAGCTTAGGAAGGGATTAGTGATCAAAGCTAAACCAGTGCCTAGTTTTTATTATGAAGGACCACCACCTAAGGTTGAACTCAAAAAGTTGCCATTAACTCGACCAAAGTCTCCAAATCTAACTCGAAGGAAAAGCTGCGGTGATGCGACGATGAGCATAGGCCTAGAGGAGAAGGGAAGAGGATGCGCACGAGCGCAGCGCCTTAGCTACGGTAGTCACAAATTTGAATCAGCTAACGGCATACCGTGCAGAAATAAAGCGCAAGCCAATGGGCATTGCTACAGTAATGAAAATTTTAAACATCGAAACCACGTGAAGCAGCATGATAAAGAGACGAGAAAAACGACTCTTGCAACGACCGATCCGCTCAAAAGTAATGTATGTATCCCGATTCACTCATGA

Protein sequence

MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQETLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVVDVSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKKHHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERIEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKSCGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKHRNHVKQHDKETRKTTLATTDPLKSNVCIPIHS
Homology
BLAST of CmaCh12G011320 vs. ExPASy Swiss-Prot
Match: Q9ASW8 (Protein WVD2-like 2 OS=Arabidopsis thaliana OX=3702 GN=WDL2 PE=2 SV=1)

HSP 1 Score: 162.9 bits (411), Expect = 8.2e-39
Identity = 106/202 (52.48%), Postives = 125/202 (61.88%), Query Frame = 0

Query: 198 NSLPNSPRPSWKSSQNDLKKNHEEQPNSP-QTSSKSSHIYLKKHHGEEEHWSTAASTFAS 257
           NSL N       +S N    +   Q NSP  T     H   K HH EE+ +S A+S+  S
Sbjct: 101 NSLGNG------ASHNSSSASRVSQLNSPLPTRRIPDH---KMHHDEEDSFSVASSSATS 160

Query: 258 IRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERIEYEARIKEEQQAAIKE 317
           IR  K K+TIG APTF S  R ++R+E+Y KLEEK KALEAE+ E E R+KEEQ+A  K+
Sbjct: 161 IRSFKPKITIGVAPTFSSTSRLERRREFYQKLEEKQKALEAEKRENEKRLKEEQEAVTKQ 220

Query: 318 LRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKSCGDATMSIGLEEKGRG 377
           LRK +  KA PVPSFY EGPPPK  LKK PLTRPKSPNL RRKSC D   +   E KG+ 
Sbjct: 221 LRKNMAYKANPVPSFYQEGPPPKQPLKKFPLTRPKSPNLNRRKSCSDTVNASYQEVKGKH 280

Query: 378 CARAQRLSYGSHKFE-SANGIP 398
           CAR  R S G  K E   N +P
Sbjct: 281 CAR-HRHSVGGCKDEVKTNSVP 292

BLAST of CmaCh12G011320 vs. ExPASy Swiss-Prot
Match: Q84ZT9 (Protein WAVE-DAMPENED 2 OS=Arabidopsis thaliana OX=3702 GN=WVD2 PE=2 SV=1)

HSP 1 Score: 153.3 bits (386), Expect = 6.5e-36
Identity = 96/164 (58.54%), Postives = 114/164 (69.51%), Query Frame = 0

Query: 210 SSQNDLKKNHEEQPNSPQTSSKSSHIYLKKHHGEEEHWSTAASTFASIRQLKSKVTIGTA 269
           + +N L +NH+      +  SK      KK+  EE+  S A    +S++  KSKVT GTA
Sbjct: 37  TEENSLSQNHKSSNVITENDSK------KKNLDEEDDCSVA----SSMKNAKSKVTHGTA 96

Query: 270 PTFQSAKRADKRKEYYNKLEEKHKALEAERIEYEARIKEEQQAAIKELRKGLVIKAKPVP 329
           P F+SA+RA+KRKEYY KLEEKH+ALEAERIE E R KEEQ+AAIK+LRK L  KA PVP
Sbjct: 97  PRFRSAQRAEKRKEYYQKLEEKHQALEAERIELEQRQKEEQEAAIKQLRKNLKFKANPVP 156

Query: 330 SFYYEGPPPKVELKKLPLTRPKSP--NLTRRKSCGDATMSIGLE 372
            FYY+ PP K ELKK PLTRPKSP  NL+RRKSC DA  S G E
Sbjct: 157 DFYYQRPPVKPELKKFPLTRPKSPKLNLSRRKSCSDAITSSGEE 190

BLAST of CmaCh12G011320 vs. ExPASy Swiss-Prot
Match: Q8GYX9 (Protein WVD2-like 1 OS=Arabidopsis thaliana OX=3702 GN=WDL1 PE=1 SV=1)

HSP 1 Score: 144.8 bits (364), Expect = 2.3e-33
Identity = 113/272 (41.54%), Postives = 152/272 (55.88%), Query Frame = 0

Query: 118 RTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTT 177
           R V + + D N  +S   + V PKI       ++   + + K     +++ +E AP    
Sbjct: 3   REVVEVLMDRNADVSSARVHVAPKIA-----AEETDEEFEVKECTEEKSL-SENAP---- 62

Query: 178 VVDVSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYL 237
             +V S       KSP  +  +         K S+ D       +P  P+          
Sbjct: 63  --NVGSAERVGAQKSPKTRNGNA--------KVSKQDAPLLAVRKPLQPEN--------- 122

Query: 238 KKHHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEA 297
           KKH  +E++ S A+S   S+R  KS +T G+APTF+SA+RA+KRKEYY KLEEK++ALEA
Sbjct: 123 KKHIDDEDNCSIASSVATSMRMGKSGLTYGSAPTFRSAQRAEKRKEYYQKLEEKNQALEA 182

Query: 298 ERIEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPN--L 357
           ER E E R K+EQ+AA+K+LRK L  KAKPVP+FYYE PP K ELKKLPLTRPKSP   L
Sbjct: 183 ERNELEQRQKDEQEAALKQLRKNLKFKAKPVPNFYYEAPPAKPELKKLPLTRPKSPKLIL 242

Query: 358 TRRKSCGDATMSIGLEEKGRGCARAQRLSYGS 388
           +RRKS  DA  S   EE  +  +   R S G+
Sbjct: 243 SRRKSFSDAVSSSSREEILKTVSNRNRHSTGT 245

BLAST of CmaCh12G011320 vs. ExPASy Swiss-Prot
Match: Q84WL6 (Protein WVD2-like 3 OS=Arabidopsis thaliana OX=3702 GN=WDL3 PE=1 SV=1)

HSP 1 Score: 117.5 bits (293), Expect = 4.0e-25
Identity = 65/121 (53.72%), Postives = 86/121 (71.07%), Query Frame = 0

Query: 238 KKHHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEA 297
           KK   EE+  S A+   +  +  KS+  +  AP+F+S +RA+KRKE+Y KLEEKH+A+EA
Sbjct: 162 KKLSDEEDSCSVASYATSGAKSAKSRTVVTAAPSFRSTERAEKRKEFYTKLEEKHQAMEA 221

Query: 298 ERIEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTR 357
           E+ + EAR KE  +AA+++LRK L  KA P+P FY+EGPPPKVELKK   TR KSP L R
Sbjct: 222 EKTQSEARNKEATEAALRQLRKSLRFKANPMPKFYHEGPPPKVELKKPLPTRAKSPKLGR 281

Query: 358 R 359
           R
Sbjct: 282 R 282

BLAST of CmaCh12G011320 vs. ExPASy Swiss-Prot
Match: Q9SJ62 (Protein WVD2-like 4 OS=Arabidopsis thaliana OX=3702 GN=WDL4 PE=1 SV=2)

HSP 1 Score: 104.4 bits (259), Expect = 3.5e-21
Identity = 66/123 (53.66%), Postives = 83/123 (67.48%), Query Frame = 0

Query: 243 EEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERIEY 302
           +E+  ST  ST ++ R  +S V   +  +F+  +RA+KRKE+Y KLEEK  A E E+   
Sbjct: 220 DEDTRSTTTST-STPRGRRSSVGSASGFSFRLEERAEKRKEFYMKLEEKIHAKEVEKTNL 279

Query: 303 EARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKSCG 362
           +A+ KE Q+  IK LRK L  KA P+PSFY E PPPKVELKK+P TRPKSP L RRKS  
Sbjct: 280 QAKSKESQEEEIKRLRKSLTFKAGPMPSFYKE-PPPKVELKKIPTTRPKSPKLGRRKSSS 339

Query: 363 DAT 366
           DAT
Sbjct: 340 DAT 340

BLAST of CmaCh12G011320 vs. ExPASy TrEMBL
Match: A0A6J1HMH4 (protein WVD2-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111465541 PE=3 SV=1)

HSP 1 Score: 847.4 bits (2188), Expect = 2.7e-242
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE 60
           MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE
Sbjct: 1   MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE 60

Query: 61  TLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERTV 120
           TLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERTV
Sbjct: 61  TLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERTV 120

Query: 121 AQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVVD 180
           AQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVVD
Sbjct: 121 AQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVVD 180

Query: 181 VSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKKH 240
           VSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKKH
Sbjct: 181 VSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKKH 240

Query: 241 HGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERI 300
           HGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERI
Sbjct: 241 HGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERI 300

Query: 301 EYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKS 360
           EYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKS
Sbjct: 301 EYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKS 360

Query: 361 CGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKHRNHV 420
           CGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKHRNHV
Sbjct: 361 CGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKHRNHV 420

Query: 421 KQHDKETRKTTLATTDPLKSNVCIPIHS 449
           KQHDKETRKTTLATTDPLKSNVCIPIHS
Sbjct: 421 KQHDKETRKTTLATTDPLKSNVCIPIHS 448

BLAST of CmaCh12G011320 vs. ExPASy TrEMBL
Match: A0A6J1FIF3 (protein WVD2-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111444228 PE=3 SV=1)

HSP 1 Score: 796.6 bits (2056), Expect = 5.4e-227
Identity = 426/452 (94.25%), Postives = 438/452 (96.90%), Query Frame = 0

Query: 1   MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE 60
           MGREIACVVE+PNDLEMVLNGVSHDKVH SSKTSEESIDLEEFEDNESSEENSLDENRQE
Sbjct: 1   MGREIACVVENPNDLEMVLNGVSHDKVHGSSKTSEESIDLEEFEDNESSEENSLDENRQE 60

Query: 61  TLDGVLAIKTTIIDASVPIPVPLLAPA----SELEPKPEPEPEPELVPVPLLVPEEKKKE 120
           TLDGVLAIKTTIIDASVPIPVPLLAPA     E EP+PEPEPEPELVPVP+LVPEEKKKE
Sbjct: 61  TLDGVLAIKTTIIDASVPIPVPLLAPAPEHEPEHEPEPEPEPEPELVPVPVLVPEEKKKE 120

Query: 121 ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPT 180
           ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQV RKGAMYAQTIETERAPTPT
Sbjct: 121 ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVLRKGAMYAQTIETERAPTPT 180

Query: 181 TVVDVSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIY 240
           TVVDVSSNAITSNSKSPGPK+NSLPNSPRPSWKSSQNDLKKNHEEQPNSPQ+SSKSSHIY
Sbjct: 181 TVVDVSSNAITSNSKSPGPKDNSLPNSPRPSWKSSQNDLKKNHEEQPNSPQSSSKSSHIY 240

Query: 241 LKKHHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALE 300
           LKKHHGEEEHWSTAASTF SIRQLKSKVTIGTAPTF+SAKRADKRKEYYNKLEEKHKALE
Sbjct: 241 LKKHHGEEEHWSTAASTFTSIRQLKSKVTIGTAPTFRSAKRADKRKEYYNKLEEKHKALE 300

Query: 301 AERIEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLT 360
           AERIEYEARIKEEQQAA+KELRKGLVIKA+PVPSFYYEGPPPKVELKKLPLTRPKSPNLT
Sbjct: 301 AERIEYEARIKEEQQAALKELRKGLVIKARPVPSFYYEGPPPKVELKKLPLTRPKSPNLT 360

Query: 361 RRKSCGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKH 420
           RRKSCGDATM+IGLEEKGRGCARAQRLSYGSHK+ESANGIPCRNKAQANGHCY+N     
Sbjct: 361 RRKSCGDATMNIGLEEKGRGCARAQRLSYGSHKYESANGIPCRNKAQANGHCYNN----- 420

Query: 421 RNHVKQHDKETRKTTLATTDPLKSNVCIPIHS 449
           RN+VKQHDKETRKTTLATTDPLKSNVCIPIHS
Sbjct: 421 RNYVKQHDKETRKTTLATTDPLKSNVCIPIHS 447

BLAST of CmaCh12G011320 vs. ExPASy TrEMBL
Match: A0A5D3D4H0 (Protein WVD2-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G001620 PE=3 SV=1)

HSP 1 Score: 501.1 bits (1289), Expect = 4.7e-138
Identity = 299/451 (66.30%), Postives = 340/451 (75.39%), Query Frame = 0

Query: 1   MGREIA-CVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQ 60
           MGREIA  VVE PN L +VLNGVSH+K H S K SEESIDLEEFE+ ES EENS  EN Q
Sbjct: 1   MGREIAGGVVEKPNGLVVVLNGVSHEKAHVSPKISEESIDLEEFEEKESGEENSFAENYQ 60

Query: 61  ETLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERT 120
           ET   V AIK++ +D SVP PV +  P S  EP P P P P   P P+LV EEKKKEERT
Sbjct: 61  ETEHDVAAIKSSNLDTSVPAPVTVPVPVS--EPVPAPPPPPAPAPAPVLVLEEKKKEERT 120

Query: 121 VAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVV 180
           VAQKISDFNKSISPGTIA+TPKI+RVNHKIQQP +Q   K    +QTIETER  T   VV
Sbjct: 121 VAQKISDFNKSISPGTIAITPKIMRVNHKIQQPPMQAPEKAVTCSQTIETERTSTTIPVV 180

Query: 181 DVSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKK 240
           + S N I  +   P P++NS PNSP+ S KSSQNDLKK+H                    
Sbjct: 181 EASPNTIELH--PPSPEKNSHPNSPQSSCKSSQNDLKKHH-------------------- 240

Query: 241 HHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAER 300
              EE+HWS A+ST  SIRQLKSKVTIGTAPTF+SA+RA KRKE+YNKLEEKHKA+EAER
Sbjct: 241 ---EEDHWSIASSTVQSIRQLKSKVTIGTAPTFRSAERAGKRKEFYNKLEEKHKAMEAER 300

Query: 301 IEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRK 360
           I+YEARIKEEQ+AAIK+LRKGLVIKA PVPSFYYEGPPPK ELKKLPLTRPKSPNLTRRK
Sbjct: 301 IQYEARIKEEQEAAIKQLRKGLVIKANPVPSFYYEGPPPKTELKKLPLTRPKSPNLTRRK 360

Query: 361 SCGDATMSIGLEEKGRGCARAQRLSYGSHKFE--SANGIPCRNKAQANGHCYSNENFKHR 420
           SCGD +M+  +EEKG+ C R  R S+ S K E  SANGI  ++KAQ NG C++NE+FKHR
Sbjct: 361 SCGD-SMNFSIEEKGKLCTRGHRHSFSSQKSEESSANGIARKSKAQVNGQCHNNESFKHR 420

Query: 421 NHVKQHDKETRKTTLATTDPLKSNVCIPIHS 449
           NHVK+ ++ET+KTTLATTDPL SNV IPI S
Sbjct: 421 NHVKR-ERETKKTTLATTDPLTSNVDIPIQS 422

BLAST of CmaCh12G011320 vs. ExPASy TrEMBL
Match: A0A1S3C3Z4 (protein WVD2-like 1 OS=Cucumis melo OX=3656 GN=LOC103496211 PE=3 SV=1)

HSP 1 Score: 500.4 bits (1287), Expect = 8.0e-138
Identity = 299/451 (66.30%), Postives = 340/451 (75.39%), Query Frame = 0

Query: 1   MGREIA-CVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQ 60
           MGREIA  VVE PN L +VLNGVSH+K H S K SEESIDLEEFE+ ES EENS  EN Q
Sbjct: 1   MGREIAGGVVEKPNGLVVVLNGVSHEKAHVSPKISEESIDLEEFEEKESGEENSFAENYQ 60

Query: 61  ETLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERT 120
           ET   V AIK++ +DASVP PV +  P SE    P P P P   P P+LV EEKKKEERT
Sbjct: 61  ETEHDVAAIKSSNLDASVPAPVTVPVPVSE----PVPAPPPAPAPAPVLVLEEKKKEERT 120

Query: 121 VAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVV 180
           VAQKISDFNKSISPGTIA+TPKI+RVNHKIQQP +Q   K    +QTIETER  T   VV
Sbjct: 121 VAQKISDFNKSISPGTIAITPKIMRVNHKIQQPPMQAPEKAVTCSQTIETERTSTTIPVV 180

Query: 181 DVSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKK 240
           + S N I  +   P P++NS PNSP+ S KSSQNDLKK+H                    
Sbjct: 181 EASPNTIELH--PPSPEKNSHPNSPQSSCKSSQNDLKKHH-------------------- 240

Query: 241 HHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAER 300
              EE+HWS A+ST  SIRQLKSKVTIGTAPTF+SA+RA KRKE+YNKLEEKHKA+EAER
Sbjct: 241 ---EEDHWSIASSTVQSIRQLKSKVTIGTAPTFRSAERAGKRKEFYNKLEEKHKAMEAER 300

Query: 301 IEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRK 360
           I+YEARIKEEQ+AAIK+LRKGLVIKA PVPSFYYEGPPPK ELKKLPLTRPKSPNLTRRK
Sbjct: 301 IQYEARIKEEQEAAIKQLRKGLVIKANPVPSFYYEGPPPKTELKKLPLTRPKSPNLTRRK 360

Query: 361 SCGDATMSIGLEEKGRGCARAQRLSYGSHKFE--SANGIPCRNKAQANGHCYSNENFKHR 420
           SCGD +M+  +EEKG+ C R  R S+ S K E  SANGI  ++KAQ NG C++NE+FKHR
Sbjct: 361 SCGD-SMNFSIEEKGKLCTRGHRHSFSSQKSEESSANGIARKSKAQVNGQCHNNESFKHR 420

Query: 421 NHVKQHDKETRKTTLATTDPLKSNVCIPIHS 449
           NHVK+ ++ET+KTTLATTDPL SNV IPI S
Sbjct: 421 NHVKR-ERETKKTTLATTDPLTSNVDIPIQS 420

BLAST of CmaCh12G011320 vs. ExPASy TrEMBL
Match: A0A0A0LIB5 (TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010250 PE=3 SV=1)

HSP 1 Score: 483.0 bits (1242), Expect = 1.3e-132
Identity = 291/450 (64.67%), Postives = 335/450 (74.44%), Query Frame = 0

Query: 1   MGREI-ACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQ 60
           MGREI   V+E PNDL +VLNGVSH+K H S K SEESIDLEE+E+ ESSEEN   EN Q
Sbjct: 34  MGREIVGDVLEKPNDLVVVLNGVSHEKAHVSPKISEESIDLEEYEEKESSEENLFPENYQ 93

Query: 61  ETLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERT 120
           ET   V AIK++ +DASVP PV +  PA      P P P P   P  +LV EEKKKEERT
Sbjct: 94  ETDHDVAAIKSSNLDASVPAPVTMPVPA------PPPPPAPAPTPAAVLVLEEKKKEERT 153

Query: 121 VAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVV 180
           VAQKISDFNKSISPGT+AVTPKI+RVNHKIQQP VQ   K  M +QTIETE  PT TTV 
Sbjct: 154 VAQKISDFNKSISPGTVAVTPKIMRVNHKIQQPPVQEPEKAVMCSQTIETE--PTSTTVP 213

Query: 181 DVSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKK 240
            V ++  T   + P P++NS PNSP+ S KSSQNDL K+H                    
Sbjct: 214 VVEASPNTIELQPPSPEKNSHPNSPQSSSKSSQNDLTKHH-------------------- 273

Query: 241 HHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAER 300
              EE+HWS A+ST  SIRQLKSKVTIG APTF+SA+RA KRKE+YNKLEEKHKA+EAER
Sbjct: 274 ---EEDHWSIASSTVQSIRQLKSKVTIGMAPTFRSAERAGKRKEFYNKLEEKHKAMEAER 333

Query: 301 IEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRK 360
           ++YEARIKEEQ+AAIK+LRKGLVIKA PVPSFYYEGPPPK ELKKLPLTRPKSPNLTRRK
Sbjct: 334 VQYEARIKEEQEAAIKQLRKGLVIKANPVPSFYYEGPPPKTELKKLPLTRPKSPNLTRRK 393

Query: 361 SCGDATMSIGLEEKGRGCARAQRLSYGSHKF-ESANGIPCRNKAQANGHCYSNENFKHRN 420
           SCGD +M+  +EEKG+ C R QR S+ S K  ES NG+  ++KAQ NG  ++NE+FKHRN
Sbjct: 394 SCGD-SMNFSIEEKGKLCTRGQRHSFSSQKSEESTNGVARKSKAQVNGQSHNNESFKHRN 450

Query: 421 HVKQHDKETRKTTLATTDPLKSNVCIPIHS 449
           HVK+ D+ET+KTT ATTDP  SNV IPI S
Sbjct: 454 HVKR-DRETKKTTFATTDPHTSNVDIPIQS 450

BLAST of CmaCh12G011320 vs. NCBI nr
Match: XP_022965736.1 (protein WVD2-like 1 [Cucurbita maxima])

HSP 1 Score: 847.4 bits (2188), Expect = 5.5e-242
Identity = 448/448 (100.00%), Postives = 448/448 (100.00%), Query Frame = 0

Query: 1   MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE 60
           MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE
Sbjct: 1   MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE 60

Query: 61  TLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERTV 120
           TLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERTV
Sbjct: 61  TLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERTV 120

Query: 121 AQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVVD 180
           AQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVVD
Sbjct: 121 AQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVVD 180

Query: 181 VSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKKH 240
           VSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKKH
Sbjct: 181 VSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKKH 240

Query: 241 HGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERI 300
           HGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERI
Sbjct: 241 HGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERI 300

Query: 301 EYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKS 360
           EYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKS
Sbjct: 301 EYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKS 360

Query: 361 CGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKHRNHV 420
           CGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKHRNHV
Sbjct: 361 CGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKHRNHV 420

Query: 421 KQHDKETRKTTLATTDPLKSNVCIPIHS 449
           KQHDKETRKTTLATTDPLKSNVCIPIHS
Sbjct: 421 KQHDKETRKTTLATTDPLKSNVCIPIHS 448

BLAST of CmaCh12G011320 vs. NCBI nr
Match: XP_022938005.1 (protein WVD2-like 1 [Cucurbita moschata])

HSP 1 Score: 796.6 bits (2056), Expect = 1.1e-226
Identity = 426/452 (94.25%), Postives = 438/452 (96.90%), Query Frame = 0

Query: 1   MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE 60
           MGREIACVVE+PNDLEMVLNGVSHDKVH SSKTSEESIDLEEFEDNESSEENSLDENRQE
Sbjct: 1   MGREIACVVENPNDLEMVLNGVSHDKVHGSSKTSEESIDLEEFEDNESSEENSLDENRQE 60

Query: 61  TLDGVLAIKTTIIDASVPIPVPLLAPA----SELEPKPEPEPEPELVPVPLLVPEEKKKE 120
           TLDGVLAIKTTIIDASVPIPVPLLAPA     E EP+PEPEPEPELVPVP+LVPEEKKKE
Sbjct: 61  TLDGVLAIKTTIIDASVPIPVPLLAPAPEHEPEHEPEPEPEPEPELVPVPVLVPEEKKKE 120

Query: 121 ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPT 180
           ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQV RKGAMYAQTIETERAPTPT
Sbjct: 121 ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVLRKGAMYAQTIETERAPTPT 180

Query: 181 TVVDVSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIY 240
           TVVDVSSNAITSNSKSPGPK+NSLPNSPRPSWKSSQNDLKKNHEEQPNSPQ+SSKSSHIY
Sbjct: 181 TVVDVSSNAITSNSKSPGPKDNSLPNSPRPSWKSSQNDLKKNHEEQPNSPQSSSKSSHIY 240

Query: 241 LKKHHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALE 300
           LKKHHGEEEHWSTAASTF SIRQLKSKVTIGTAPTF+SAKRADKRKEYYNKLEEKHKALE
Sbjct: 241 LKKHHGEEEHWSTAASTFTSIRQLKSKVTIGTAPTFRSAKRADKRKEYYNKLEEKHKALE 300

Query: 301 AERIEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLT 360
           AERIEYEARIKEEQQAA+KELRKGLVIKA+PVPSFYYEGPPPKVELKKLPLTRPKSPNLT
Sbjct: 301 AERIEYEARIKEEQQAALKELRKGLVIKARPVPSFYYEGPPPKVELKKLPLTRPKSPNLT 360

Query: 361 RRKSCGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKH 420
           RRKSCGDATM+IGLEEKGRGCARAQRLSYGSHK+ESANGIPCRNKAQANGHCY+N     
Sbjct: 361 RRKSCGDATMNIGLEEKGRGCARAQRLSYGSHKYESANGIPCRNKAQANGHCYNN----- 420

Query: 421 RNHVKQHDKETRKTTLATTDPLKSNVCIPIHS 449
           RN+VKQHDKETRKTTLATTDPLKSNVCIPIHS
Sbjct: 421 RNYVKQHDKETRKTTLATTDPLKSNVCIPIHS 447

BLAST of CmaCh12G011320 vs. NCBI nr
Match: XP_023538034.1 (protein WVD2-like 1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 795.8 bits (2054), Expect = 1.9e-226
Identity = 425/452 (94.03%), Postives = 437/452 (96.68%), Query Frame = 0

Query: 1   MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE 60
           MGREIACVVE+PNDLEMVLNGVSHDKVH SSKTSEESIDLEEFEDNESSEENSLDENRQ+
Sbjct: 1   MGREIACVVENPNDLEMVLNGVSHDKVHGSSKTSEESIDLEEFEDNESSEENSLDENRQD 60

Query: 61  TLDGVLAIKTTIIDASVPIPVPLLAPASELEPK----PEPEPEPELVPVPLLVPEEKKKE 120
           T DGVLAIKTTIIDASVPIPVPLLAPA ELEPK    PEPEPEPELVPVP+LVPEEKKKE
Sbjct: 61  TQDGVLAIKTTIIDASVPIPVPLLAPAPELEPKPEPEPEPEPEPELVPVPVLVPEEKKKE 120

Query: 121 ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPT 180
           ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQV RKGAMYAQTIETERAPTPT
Sbjct: 121 ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVPRKGAMYAQTIETERAPTPT 180

Query: 181 TVVDVSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIY 240
           TVVDVSSN ITSNSKSPGPK+NSLPNSPRPSWKSSQNDLKKNHEEQPNSPQ+SSKSSHIY
Sbjct: 181 TVVDVSSNVITSNSKSPGPKDNSLPNSPRPSWKSSQNDLKKNHEEQPNSPQSSSKSSHIY 240

Query: 241 LKKHHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALE 300
           LKKHHGEEEHWSTAASTF SIRQLKSKVTIGTAPTF+SAKRADKRKEYYNKLEEKHKALE
Sbjct: 241 LKKHHGEEEHWSTAASTFTSIRQLKSKVTIGTAPTFRSAKRADKRKEYYNKLEEKHKALE 300

Query: 301 AERIEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLT 360
           AERIEYEARIKEEQQAA+KELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLT
Sbjct: 301 AERIEYEARIKEEQQAALKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLT 360

Query: 361 RRKSCGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKH 420
           RRKSCGDATM+IGLEEKGRGCARAQRLSYGSHK+ESANGIPCRNKAQANGHCY+N     
Sbjct: 361 RRKSCGDATMNIGLEEKGRGCARAQRLSYGSHKYESANGIPCRNKAQANGHCYNN----- 420

Query: 421 RNHVKQHDKETRKTTLATTDPLKSNVCIPIHS 449
           RN+VKQHDKETRKTTLAT+DPLKSNVCIPIHS
Sbjct: 421 RNYVKQHDKETRKTTLATSDPLKSNVCIPIHS 447

BLAST of CmaCh12G011320 vs. NCBI nr
Match: KAG6586318.1 (Protein WVD2-like 2, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 792.7 bits (2046), Expect = 1.6e-225
Identity = 425/452 (94.03%), Postives = 436/452 (96.46%), Query Frame = 0

Query: 1   MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE 60
           MGREIACVVE+PNDLEMVLNGVSHDKVH SSKTSEESIDLEEFEDNE SEENSLDENRQE
Sbjct: 1   MGREIACVVENPNDLEMVLNGVSHDKVHGSSKTSEESIDLEEFEDNELSEENSLDENRQE 60

Query: 61  TLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPE----LVPVPLLVPEEKKKE 120
           TLDGVLAIKTTIIDASVPIPVPLLAPA E EP+PEPEPEPE     VPVP+LVPEEKKKE
Sbjct: 61  TLDGVLAIKTTIIDASVPIPVPLLAPAPEHEPEPEPEPEPEPELVPVPVPVLVPEEKKKE 120

Query: 121 ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPT 180
           ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQV RKGAMYAQTIETERAPTPT
Sbjct: 121 ERTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVLRKGAMYAQTIETERAPTPT 180

Query: 181 TVVDVSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIY 240
           TVVDVSSNAITSNSKSPGPK+NSLPNSPRPSWKSSQNDLKKNHEEQPNSPQ+SSKSSHIY
Sbjct: 181 TVVDVSSNAITSNSKSPGPKDNSLPNSPRPSWKSSQNDLKKNHEEQPNSPQSSSKSSHIY 240

Query: 241 LKKHHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALE 300
           LKKHHGEEEHWSTAASTF SIRQLKSKVTIGTAPTF+SAKRADKRKEYYNKLEEKHKALE
Sbjct: 241 LKKHHGEEEHWSTAASTFTSIRQLKSKVTIGTAPTFRSAKRADKRKEYYNKLEEKHKALE 300

Query: 301 AERIEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLT 360
           AERIEYEARIKEEQQAA+KELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLT
Sbjct: 301 AERIEYEARIKEEQQAALKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLT 360

Query: 361 RRKSCGDATMSIGLEEKGRGCARAQRLSYGSHKFESANGIPCRNKAQANGHCYSNENFKH 420
           RRKSCGDATM+IGLEEKGRGCARAQRLSYGSHK+ESANGIPCRNKAQANGHCY+N     
Sbjct: 361 RRKSCGDATMNIGLEEKGRGCARAQRLSYGSHKYESANGIPCRNKAQANGHCYNN----- 420

Query: 421 RNHVKQHDKETRKTTLATTDPLKSNVCIPIHS 449
           RN+VKQHDKETRKTTLATTDPLKSNVCIPIHS
Sbjct: 421 RNYVKQHDKETRKTTLATTDPLKSNVCIPIHS 447

BLAST of CmaCh12G011320 vs. NCBI nr
Match: XP_038890840.1 (protein WVD2-like 2 [Benincasa hispida] >XP_038890841.1 protein WVD2-like 2 [Benincasa hispida])

HSP 1 Score: 506.5 bits (1303), Expect = 2.3e-139
Identity = 301/452 (66.59%), Postives = 341/452 (75.44%), Query Frame = 0

Query: 1   MGREIACVVEDPNDLEMVLNGVSHDKVHDSSKTSEESIDLEEFEDNESSEENSLDENRQE 60
           MGREIA VVE PN L +VLNGVSHDKVH S K +EESID EEFE+ ESSEENS   N QE
Sbjct: 1   MGREIAGVVEKPNALVVVLNGVSHDKVHVSPKIAEESIDSEEFEEKESSEENSFAGNYQE 60

Query: 61  TLDGVLAIKTTIIDASVPIPVPLLAPASELEPKPEPEPEPELVPVPLLVPEEKKKEERTV 120
           T   V+AIK++ +DASVP PV +                   VP P++VPEEKKKEERTV
Sbjct: 61  TEHDVVAIKSSNLDASVPAPVTV------------------PVPAPVVVPEEKKKEERTV 120

Query: 121 AQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTTVVD 180
           AQKISDFNKSISPGTIA TPKI++VNHKIQQPTVQ   K A  +QTIETE  PT  T+V+
Sbjct: 121 AQKISDFNKSISPGTIAATPKIMKVNHKIQQPTVQAPEKAATCSQTIETEPTPTTITIVE 180

Query: 181 VSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYLKKH 240
            S N I    + P PK+NS PNSP+ S KSSQND KK+H                     
Sbjct: 181 ASPNTI--ELQPPSPKKNSRPNSPQSSGKSSQNDFKKHH--------------------- 240

Query: 241 HGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERI 300
             EE+HWS A+ST  SIRQLKSKVTIGTAPTF+SA+RADKRKE+YNKLEEKHKALEAERI
Sbjct: 241 --EEDHWSIASSTVQSIRQLKSKVTIGTAPTFRSAERADKRKEFYNKLEEKHKALEAERI 300

Query: 301 EYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKS 360
           +YEARIKEEQ+AAIK+LRKGLVIKA PVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKS
Sbjct: 301 QYEARIKEEQEAAIKQLRKGLVIKANPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKS 360

Query: 361 CGDATMSIGLEEKGRGCARAQRLSYGSHKF-ESANGIPCRNKAQANGHCYSNENFKHRNH 420
           C D +M++ +EEKG+ C RAQ  S+ S K  ESANGI C++KAQANGHC++NE+FKHRNH
Sbjct: 361 CSD-SMNLSVEEKGKVCTRAQHHSFSSQKADESANGIACKSKAQANGHCHNNESFKHRNH 407

Query: 421 VKQHDKETRKTTLATT---DPLKSNVCIPIHS 449
           VK+ +KETRK T ATT   DP KSNV IPI S
Sbjct: 421 VKR-NKETRKMTFATTDPHDPHKSNVDIPIRS 407

BLAST of CmaCh12G011320 vs. TAIR 10
Match: AT1G54460.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 162.9 bits (411), Expect = 5.8e-40
Identity = 106/202 (52.48%), Postives = 125/202 (61.88%), Query Frame = 0

Query: 198 NSLPNSPRPSWKSSQNDLKKNHEEQPNSP-QTSSKSSHIYLKKHHGEEEHWSTAASTFAS 257
           NSL N       +S N    +   Q NSP  T     H   K HH EE+ +S A+S+  S
Sbjct: 101 NSLGNG------ASHNSSSASRVSQLNSPLPTRRIPDH---KMHHDEEDSFSVASSSATS 160

Query: 258 IRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEAERIEYEARIKEEQQAAIKE 317
           IR  K K+TIG APTF S  R ++R+E+Y KLEEK KALEAE+ E E R+KEEQ+A  K+
Sbjct: 161 IRSFKPKITIGVAPTFSSTSRLERRREFYQKLEEKQKALEAEKRENEKRLKEEQEAVTKQ 220

Query: 318 LRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPNLTRRKSCGDATMSIGLEEKGRG 377
           LRK +  KA PVPSFY EGPPPK  LKK PLTRPKSPNL RRKSC D   +   E KG+ 
Sbjct: 221 LRKNMAYKANPVPSFYQEGPPPKQPLKKFPLTRPKSPNLNRRKSCSDTVNASYQEVKGKH 280

Query: 378 CARAQRLSYGSHKFE-SANGIP 398
           CAR  R S G  K E   N +P
Sbjct: 281 CAR-HRHSVGGCKDEVKTNSVP 292

BLAST of CmaCh12G011320 vs. TAIR 10
Match: AT5G28646.1 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 153.3 bits (386), Expect = 4.6e-37
Identity = 96/164 (58.54%), Postives = 114/164 (69.51%), Query Frame = 0

Query: 210 SSQNDLKKNHEEQPNSPQTSSKSSHIYLKKHHGEEEHWSTAASTFASIRQLKSKVTIGTA 269
           + +N L +NH+      +  SK      KK+  EE+  S A    +S++  KSKVT GTA
Sbjct: 37  TEENSLSQNHKSSNVITENDSK------KKNLDEEDDCSVA----SSMKNAKSKVTHGTA 96

Query: 270 PTFQSAKRADKRKEYYNKLEEKHKALEAERIEYEARIKEEQQAAIKELRKGLVIKAKPVP 329
           P F+SA+RA+KRKEYY KLEEKH+ALEAERIE E R KEEQ+AAIK+LRK L  KA PVP
Sbjct: 97  PRFRSAQRAEKRKEYYQKLEEKHQALEAERIELEQRQKEEQEAAIKQLRKNLKFKANPVP 156

Query: 330 SFYYEGPPPKVELKKLPLTRPKSP--NLTRRKSCGDATMSIGLE 372
            FYY+ PP K ELKK PLTRPKSP  NL+RRKSC DA  S G E
Sbjct: 157 DFYYQRPPVKPELKKFPLTRPKSPKLNLSRRKSCSDAITSSGEE 190

BLAST of CmaCh12G011320 vs. TAIR 10
Match: AT5G28646.2 (TPX2 (targeting protein for Xklp2) protein family )

HSP 1 Score: 153.3 bits (386), Expect = 4.6e-37
Identity = 96/164 (58.54%), Postives = 114/164 (69.51%), Query Frame = 0

Query: 210 SSQNDLKKNHEEQPNSPQTSSKSSHIYLKKHHGEEEHWSTAASTFASIRQLKSKVTIGTA 269
           + +N L +NH+      +  SK      KK+  EE+  S A    +S++  KSKVT GTA
Sbjct: 37  TEENSLSQNHKSSNVITENDSK------KKNLDEEDDCSVA----SSMKNAKSKVTHGTA 96

Query: 270 PTFQSAKRADKRKEYYNKLEEKHKALEAERIEYEARIKEEQQAAIKELRKGLVIKAKPVP 329
           P F+SA+RA+KRKEYY KLEEKH+ALEAERIE E R KEEQ+AAIK+LRK L  KA PVP
Sbjct: 97  PRFRSAQRAEKRKEYYQKLEEKHQALEAERIELEQRQKEEQEAAIKQLRKNLKFKANPVP 156

Query: 330 SFYYEGPPPKVELKKLPLTRPKSP--NLTRRKSCGDATMSIGLE 372
            FYY+ PP K ELKK PLTRPKSP  NL+RRKSC DA  S G E
Sbjct: 157 DFYYQRPPVKPELKKFPLTRPKSPKLNLSRRKSCSDAITSSGEE 190

BLAST of CmaCh12G011320 vs. TAIR 10
Match: AT3G04630.1 (WVD2-like 1 )

HSP 1 Score: 151.4 bits (381), Expect = 1.8e-36
Identity = 90/152 (59.21%), Postives = 111/152 (73.03%), Query Frame = 0

Query: 238 KKHHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEA 297
           KKH  +E++ S A+S   S+R  KS +T G+APTF+SA+RA+KRKEYY KLEEK++ALEA
Sbjct: 95  KKHIDDEDNCSIASSVATSMRMGKSGLTYGSAPTFRSAQRAEKRKEYYQKLEEKNQALEA 154

Query: 298 ERIEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPN--L 357
           ER E E R K+EQ+AA+K+LRK L  KAKPVP+FYYE PP K ELKKLPLTRPKSP   L
Sbjct: 155 ERNELEQRQKDEQEAALKQLRKNLKFKAKPVPNFYYEAPPAKPELKKLPLTRPKSPKLIL 214

Query: 358 TRRKSCGDATMSIGLEEKGRGCARAQRLSYGS 388
           +RRKS  DA  S   EE  +  +   R S G+
Sbjct: 215 SRRKSFSDAVSSSSREEILKTVSNRNRHSTGT 246

BLAST of CmaCh12G011320 vs. TAIR 10
Match: AT3G04630.2 (WVD2-like 1 )

HSP 1 Score: 144.8 bits (364), Expect = 1.6e-34
Identity = 113/272 (41.54%), Postives = 152/272 (55.88%), Query Frame = 0

Query: 118 RTVAQKISDFNKSISPGTIAVTPKIVRVNHKIQQPTVQVQRKGAMYAQTIETERAPTPTT 177
           R V + + D N  +S   + V PKI       ++   + + K     +++ +E AP    
Sbjct: 3   REVVEVLMDRNADVSSARVHVAPKIA-----AEETDEEFEVKECTEEKSL-SENAP---- 62

Query: 178 VVDVSSNAITSNSKSPGPKENSLPNSPRPSWKSSQNDLKKNHEEQPNSPQTSSKSSHIYL 237
             +V S       KSP  +  +         K S+ D       +P  P+          
Sbjct: 63  --NVGSAERVGAQKSPKTRNGNA--------KVSKQDAPLLAVRKPLQPEN--------- 122

Query: 238 KKHHGEEEHWSTAASTFASIRQLKSKVTIGTAPTFQSAKRADKRKEYYNKLEEKHKALEA 297
           KKH  +E++ S A+S   S+R  KS +T G+APTF+SA+RA+KRKEYY KLEEK++ALEA
Sbjct: 123 KKHIDDEDNCSIASSVATSMRMGKSGLTYGSAPTFRSAQRAEKRKEYYQKLEEKNQALEA 182

Query: 298 ERIEYEARIKEEQQAAIKELRKGLVIKAKPVPSFYYEGPPPKVELKKLPLTRPKSPN--L 357
           ER E E R K+EQ+AA+K+LRK L  KAKPVP+FYYE PP K ELKKLPLTRPKSP   L
Sbjct: 183 ERNELEQRQKDEQEAALKQLRKNLKFKAKPVPNFYYEAPPAKPELKKLPLTRPKSPKLIL 242

Query: 358 TRRKSCGDATMSIGLEEKGRGCARAQRLSYGS 388
           +RRKS  DA  S   EE  +  +   R S G+
Sbjct: 243 SRRKSFSDAVSSSSREEILKTVSNRNRHSTGT 245

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ASW88.2e-3952.48Protein WVD2-like 2 OS=Arabidopsis thaliana OX=3702 GN=WDL2 PE=2 SV=1[more]
Q84ZT96.5e-3658.54Protein WAVE-DAMPENED 2 OS=Arabidopsis thaliana OX=3702 GN=WVD2 PE=2 SV=1[more]
Q8GYX92.3e-3341.54Protein WVD2-like 1 OS=Arabidopsis thaliana OX=3702 GN=WDL1 PE=1 SV=1[more]
Q84WL64.0e-2553.72Protein WVD2-like 3 OS=Arabidopsis thaliana OX=3702 GN=WDL3 PE=1 SV=1[more]
Q9SJ623.5e-2153.66Protein WVD2-like 4 OS=Arabidopsis thaliana OX=3702 GN=WDL4 PE=1 SV=2[more]
Match NameE-valueIdentityDescription
A0A6J1HMH42.7e-242100.00protein WVD2-like 1 OS=Cucurbita maxima OX=3661 GN=LOC111465541 PE=3 SV=1[more]
A0A6J1FIF35.4e-22794.25protein WVD2-like 1 OS=Cucurbita moschata OX=3662 GN=LOC111444228 PE=3 SV=1[more]
A0A5D3D4H04.7e-13866.30Protein WVD2-like 1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold456G... [more]
A0A1S3C3Z48.0e-13866.30protein WVD2-like 1 OS=Cucumis melo OX=3656 GN=LOC103496211 PE=3 SV=1[more]
A0A0A0LIB51.3e-13264.67TPX2 domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G010250 PE=3 S... [more]
Match NameE-valueIdentityDescription
XP_022965736.15.5e-242100.00protein WVD2-like 1 [Cucurbita maxima][more]
XP_022938005.11.1e-22694.25protein WVD2-like 1 [Cucurbita moschata][more]
XP_023538034.11.9e-22694.03protein WVD2-like 1 [Cucurbita pepo subsp. pepo][more]
KAG6586318.11.6e-22594.03Protein WVD2-like 2, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_038890840.12.3e-13966.59protein WVD2-like 2 [Benincasa hispida] >XP_038890841.1 protein WVD2-like 2 [Ben... [more]
Match NameE-valueIdentityDescription
AT1G54460.15.8e-4052.48TPX2 (targeting protein for Xklp2) protein family [more]
AT5G28646.14.6e-3758.54TPX2 (targeting protein for Xklp2) protein family [more]
AT5G28646.24.6e-3758.54TPX2 (targeting protein for Xklp2) protein family [more]
AT3G04630.11.8e-3659.21WVD2-like 1 [more]
AT3G04630.21.6e-3441.54WVD2-like 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 40..60
NoneNo IPR availableCOILSCoilCoilcoord: 278..312
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 23..37
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 23..59
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 88..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 185..244
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 185..211
NoneNo IPR availablePANTHERPTHR46372:SF5TPX2 (TARGETING PROTEIN FOR XKLP2) FAMILY PROTEINcoord: 220..448
coord: 1..221
IPR027329TPX2, C-terminalPFAMPF06886TPX2coord: 271..354
e-value: 1.4E-22
score: 79.6
IPR044806Protein WAVE-DAMPENED 2-likePANTHERPTHR46372PROTEIN WVD2-LIKE 3coord: 220..448
coord: 1..221

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G011320.1CmaCh12G011320.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0000226 microtubule cytoskeleton organization
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005874 microtubule
molecular_function GO:0008017 microtubule binding