CmaCh12G010310 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G010310
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptiontranscription termination factor MTERF4, chloroplastic
LocationCma_Chr12: 8190989 .. 8192602 (+)
RNA-Seq ExpressionCmaCh12G010310
SyntenyCmaCh12G010310
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAGTTTTTCCAGGTGCAGGGTGCAAAACCTGGAGTTATGATGATCAGAGGCCATACTGGTGTGGTAAAACCTAATTTTTCCATCATGTTACTCGAGATGCCTACCCTTAAATGTCACAAATTCAAATTTGGTACGACATCAACTTTTCAAGCGTCTCCTAACCATGAGAAAAGGGGGAGTTTGATTACAAAGTTACAATACTCTCGTGGTGATAGGAAAACAGTATCCAAATCACTGCATTCTACTATTACATCAAAGCCTTACAGTTCTAAAGTGAGTAGAAAAGAGGGAAGTGCTTCATCGTTATATAGTCGCCCTAGCTTAGTGGAAATGAAGAATGTGAGACAGGAAAATCGTGCAAAGGTTTACGAGTTCTTGCGTGGAATTGGTATTGTTCCTGATGAACTTGATGGATTGGAGCTTCCAGTAACAGTTGATGTTATGAAGGAACGTGTCGATTTTCTTCTCAAACTAGGACTTGCAATTGAAGATATAAATAACTACCCCCTTGTTCTTGGCTGCAGTGTTAAGAAAAATATGGTACCAGTACTTGATTACCTTGGTAAATTGGGCGTTAGGAAATCTACTTTCACCGAGTTTTTAAGAAGGTATCCGCAAGTCCTCCATTCCAGCGTCGTTGTGGACCTTGGTCCAGTAGTCAAATATCTTCAGGGCATGGATATTAAACCAAGTGATATCCCGCGCGTTCTTGAGAGATATCCAGAAGTATTGGGGTTTAAGCTTGAGGGAACCATGAGCACTTCAGTGGCATACTTGGTAGGAATTGGAGTGGCAAGAAGAGAAATCGGAGGGGTTTTAACCAAGTACCCTGAAATTTTGGGAATGAGGGTTGCTCGGGTAATCAAGCCTTTTGTAGAATATCTTGAAAGTTTGGGAATTCCAAGATTAGCCGTGGCTAGGTTAATAGAGAAACGACCACACATTCTCGGGTTCGGATTGGAAAAAAAGGTCAAACCCAACATCCAATTGCTCTTGGAGTTTAATGTGAGAGAAGCATATCTTTCTTCTATAGTTGCACAGTATGCCGAGATCATAGGAATGGACCTTGAGACTAAGCTTCCAAGTCAAAGGAACTTACTAAAATCAATAATCGAATTGGATAACGATAACTTCGGCACTATAATTGAAAAGATGCCACAAGTTGTTAGCCTTAGCAGTTCGGCTGTGAACAGCCATGTCGATTTCTTAAAAAGTTGTGGGTTTTCCTTGCAGCAAGTAAAGAACATGGTTATAGGTTGTCCCCAGTTGCTTGCTTTGAATCTTGACATAATGAAACACAGCTTTCAGTTCTTCCAAAAGGAAATGGGAAGGCCTTTAGACGATCTGGTTGCTTTTCCTGCATTCTTTACTTATGGTCTGGAGTCCACAATAAAGCCTAGACATAGGAAGGTTGTACATAAGGGAATTACCTGTTCTCTTTCATGGCTCCTCAATTGTTCTGATGAAAAGTTCGCTGAAAGGATGACCTACGACACCATAGATATGGAGGAGATGGAAACTGCACCATCGTTTGATATGAGTTCTCTTACACAACCCCGGTATGATGAATCGGATTCTGACTATGAAGAGGACGGCGAGGAGGAGACTTAA

mRNA sequence

ATGCAGTTTTTCCAGGTGCAGGGTGCAAAACCTGGAGTTATGATGATCAGAGGCCATACTGGTGTGGTAAAACCTAATTTTTCCATCATGTTACTCGAGATGCCTACCCTTAAATGTCACAAATTCAAATTTGGTACGACATCAACTTTTCAAGCGTCTCCTAACCATGAGAAAAGGGGGAGTTTGATTACAAAGTTACAATACTCTCGTGGTGATAGGAAAACAGTATCCAAATCACTGCATTCTACTATTACATCAAAGCCTTACAGTTCTAAAGTGAGTAGAAAAGAGGGAAGTGCTTCATCGTTATATAGTCGCCCTAGCTTAGTGGAAATGAAGAATGTGAGACAGGAAAATCGTGCAAAGGTTTACGAGTTCTTGCGTGGAATTGGTATTGTTCCTGATGAACTTGATGGATTGGAGCTTCCAGTAACAGTTGATGTTATGAAGGAACGTGTCGATTTTCTTCTCAAACTAGGACTTGCAATTGAAGATATAAATAACTACCCCCTTGTTCTTGGCTGCAGTGTTAAGAAAAATATGGTACCAGTACTTGATTACCTTGGTAAATTGGGCGTTAGGAAATCTACTTTCACCGAGTTTTTAAGAAGGTATCCGCAAGTCCTCCATTCCAGCGTCGTTGTGGACCTTGGTCCAGTAGTCAAATATCTTCAGGGCATGGATATTAAACCAAGTGATATCCCGCGCGTTCTTGAGAGATATCCAGAAGTATTGGGGTTTAAGCTTGAGGGAACCATGAGCACTTCAGTGGCATACTTGGTAGGAATTGGAGTGGCAAGAAGAGAAATCGGAGGGGTTTTAACCAAGTACCCTGAAATTTTGGGAATGAGGGTTGCTCGGGTAATCAAGCCTTTTGTAGAATATCTTGAAAGTTTGGGAATTCCAAGATTAGCCGTGGCTAGGTTAATAGAGAAACGACCACACATTCTCGGGTTCGGATTGGAAAAAAAGGTCAAACCCAACATCCAATTGCTCTTGGAGTTTAATGTGAGAGAAGCATATCTTTCTTCTATAGTTGCACAGTATGCCGAGATCATAGGAATGGACCTTGAGACTAAGCTTCCAAGTCAAAGGAACTTACTAAAATCAATAATCGAATTGGATAACGATAACTTCGGCACTATAATTGAAAAGATGCCACAAGTTGTTAGCCTTAGCAGTTCGGCTGTGAACAGCCATGTCGATTTCTTAAAAAGTTGTGGGTTTTCCTTGCAGCAAGTAAAGAACATGGTTATAGGTTGTCCCCAGTTGCTTGCTTTGAATCTTGACATAATGAAACACAGCTTTCAGTTCTTCCAAAAGGAAATGGGAAGGCCTTTAGACGATCTGGTTGCTTTTCCTGCATTCTTTACTTATGGTCTGGAGTCCACAATAAAGCCTAGACATAGGAAGGTTGTACATAAGGGAATTACCTGTTCTCTTTCATGGCTCCTCAATTGTTCTGATGAAAAGTTCGCTGAAAGGATGACCTACGACACCATAGATATGGAGGAGATGGAAACTGCACCATCGTTTGATATGAGTTCTCTTACACAACCCCGGTATGATGAATCGGATTCTGACTATGAAGAGGACGGCGAGGAGGAGACTTAA

Coding sequence (CDS)

ATGCAGTTTTTCCAGGTGCAGGGTGCAAAACCTGGAGTTATGATGATCAGAGGCCATACTGGTGTGGTAAAACCTAATTTTTCCATCATGTTACTCGAGATGCCTACCCTTAAATGTCACAAATTCAAATTTGGTACGACATCAACTTTTCAAGCGTCTCCTAACCATGAGAAAAGGGGGAGTTTGATTACAAAGTTACAATACTCTCGTGGTGATAGGAAAACAGTATCCAAATCACTGCATTCTACTATTACATCAAAGCCTTACAGTTCTAAAGTGAGTAGAAAAGAGGGAAGTGCTTCATCGTTATATAGTCGCCCTAGCTTAGTGGAAATGAAGAATGTGAGACAGGAAAATCGTGCAAAGGTTTACGAGTTCTTGCGTGGAATTGGTATTGTTCCTGATGAACTTGATGGATTGGAGCTTCCAGTAACAGTTGATGTTATGAAGGAACGTGTCGATTTTCTTCTCAAACTAGGACTTGCAATTGAAGATATAAATAACTACCCCCTTGTTCTTGGCTGCAGTGTTAAGAAAAATATGGTACCAGTACTTGATTACCTTGGTAAATTGGGCGTTAGGAAATCTACTTTCACCGAGTTTTTAAGAAGGTATCCGCAAGTCCTCCATTCCAGCGTCGTTGTGGACCTTGGTCCAGTAGTCAAATATCTTCAGGGCATGGATATTAAACCAAGTGATATCCCGCGCGTTCTTGAGAGATATCCAGAAGTATTGGGGTTTAAGCTTGAGGGAACCATGAGCACTTCAGTGGCATACTTGGTAGGAATTGGAGTGGCAAGAAGAGAAATCGGAGGGGTTTTAACCAAGTACCCTGAAATTTTGGGAATGAGGGTTGCTCGGGTAATCAAGCCTTTTGTAGAATATCTTGAAAGTTTGGGAATTCCAAGATTAGCCGTGGCTAGGTTAATAGAGAAACGACCACACATTCTCGGGTTCGGATTGGAAAAAAAGGTCAAACCCAACATCCAATTGCTCTTGGAGTTTAATGTGAGAGAAGCATATCTTTCTTCTATAGTTGCACAGTATGCCGAGATCATAGGAATGGACCTTGAGACTAAGCTTCCAAGTCAAAGGAACTTACTAAAATCAATAATCGAATTGGATAACGATAACTTCGGCACTATAATTGAAAAGATGCCACAAGTTGTTAGCCTTAGCAGTTCGGCTGTGAACAGCCATGTCGATTTCTTAAAAAGTTGTGGGTTTTCCTTGCAGCAAGTAAAGAACATGGTTATAGGTTGTCCCCAGTTGCTTGCTTTGAATCTTGACATAATGAAACACAGCTTTCAGTTCTTCCAAAAGGAAATGGGAAGGCCTTTAGACGATCTGGTTGCTTTTCCTGCATTCTTTACTTATGGTCTGGAGTCCACAATAAAGCCTAGACATAGGAAGGTTGTACATAAGGGAATTACCTGTTCTCTTTCATGGCTCCTCAATTGTTCTGATGAAAAGTTCGCTGAAAGGATGACCTACGACACCATAGATATGGAGGAGATGGAAACTGCACCATCGTTTGATATGAGTTCTCTTACACAACCCCGGTATGATGAATCGGATTCTGACTATGAAGAGGACGGCGAGGAGGAGACTTAA

Protein sequence

MQFFQVQGAKPGVMMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDRKTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIELDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMKHSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET
Homology
BLAST of CmaCh12G010310 vs. ExPASy Swiss-Prot
Match: Q9ZT96 (Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF4 PE=1 SV=1)

HSP 1 Score: 684.9 bits (1766), Expect = 7.5e-196
Identity = 360/536 (67.16%), Postives = 425/536 (79.29%), Query Frame = 0

Query: 14  MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKR--GSLITKLQYSRG 73
           M IR   G  KP F ++  E P+    + +  + ST+    NH+KR    +   +Q +  
Sbjct: 1   MKIRFCNGFTKPGFLLVHFEPPSFFAVRSRSLSDSTYGNLCNHKKRPGTGIGLTVQCAIA 60

Query: 74  DRKTVSKSLHSTITSKPYSSKVSR---------KEGSASSLYSRPSLVEMKNVRQENRAK 133
           +R+  S+SL S    +   S  S          K   + SLYSRPSL++M   +  NRAK
Sbjct: 61  NRRFSSRSLDSPRRERSSRSSSSSGRDRDRDKDKGRDSKSLYSRPSLLDMNKEKAANRAK 120

Query: 134 VYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMV 193
           VYEFLRGIGIVPDELDGLELPVT DVMKERV+FL KLGL IEDINNYPLVLGCSVKKNMV
Sbjct: 121 VYEFLRGIGIVPDELDGLELPVTADVMKERVEFLHKLGLTIEDINNYPLVLGCSVKKNMV 180

Query: 194 PVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYP 253
           PVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVV+DL PVVKYLQG+DIKPSD+PRVLERYP
Sbjct: 181 PVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVIDLAPVVKYLQGLDIKPSDVPRVLERYP 240

Query: 254 EVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIP 313
           EVLGFKLEGTMSTSVAYLVGIGVARREIGG+LT+YPEILGMRVAR+IKP VEYLE LGIP
Sbjct: 241 EVLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPEILGMRVARIIKPLVEYLEVLGIP 300

Query: 314 RLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLP 373
           RLA ARLIEKRPHILGF L+  VKPN+Q+L +FNVRE  L SI+AQY EIIG+DL+ KL 
Sbjct: 301 RLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLPSIIAQYPEIIGIDLKPKLD 360

Query: 374 SQRNLLKSIIELDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCP 433
           +QR LL S I L+ ++ G++IE+MPQ VSLS S +  H+DFL  CGFS+ Q + MVIGCP
Sbjct: 361 TQRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLKHIDFLTKCGFSIDQTREMVIGCP 420

Query: 434 QLLALNLDIMKHSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLS 493
           Q+LALNL IMK SF++FQKEM RPL DLV FPAFFTYGLEST+KPRH+K++ KGI CSL+
Sbjct: 421 QVLALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLESTVKPRHKKIIKKGIKCSLA 480

Query: 494 WLLNCSDEKFAERMTYDTIDMEEMETAP-SFDMSSLTQP-RYDESDSDYEEDGEEE 537
           W+LNCSDEKF +RM+YDTID+EE+ET P SFDM++L QP R +ESDS+YEE+ +++
Sbjct: 481 WMLNCSDEKFEQRMSYDTIDIEEVETDPSSFDMNTLMQPEREEESDSEYEEEEDDD 536

BLAST of CmaCh12G010310 vs. ExPASy Swiss-Prot
Match: Q6AUK6 (Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. japonica OX=39947 GN=Os05g0404000 PE=2 SV=1)

HSP 1 Score: 570.9 bits (1470), Expect = 1.6e-161
Identity = 281/445 (63.15%), Postives = 356/445 (80.00%), Query Frame = 0

Query: 94  SRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIVPDELDGLELPVTVDVMKERV 153
           S +   +SSLY+RPSL++M+  R   RA V  FL  +G+ P EL GLELP TVDVM+ERV
Sbjct: 57  SPRPSPSSSLYARPSLLDMERGRAARRADVDAFLASLGVDPGELAGLELPATVDVMRERV 116

Query: 154 DFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSV 213
           +FL  L L+ ED+  YPL LGCSV+KNMVPVLDYLGKLGVR+    + LRRYPQVLH+SV
Sbjct: 117 EFLHSLDLSNEDLAAYPLALGCSVRKNMVPVLDYLGKLGVRQDALPDLLRRYPQVLHASV 176

Query: 214 VVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGV 273
           VVDL PVVKYLQGMD++P D+PRVLERYPE+LGFKLEGTMSTS+AYLVGIGVARR++G V
Sbjct: 177 VVDLAPVVKYLQGMDVRPHDVPRVLERYPELLGFKLEGTMSTSIAYLVGIGVARRQVGSV 236

Query: 274 LTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLL 333
           +T++PE+LGMRV ++IKPFVE+LE +G+ RLA+AR+IEK+P++LGFGLE KVKPNI+ LL
Sbjct: 237 ITRFPEVLGMRVGKIIKPFVEHLEGIGLQRLAIARIIEKKPYVLGFGLEDKVKPNIEALL 296

Query: 334 EFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIELDNDNFGTIIEKMPQVVSLS 393
           EF VR+  L+ IVAQY +I+G++L  KL +Q++L +S I + +++FG +IE+MPQ +SL 
Sbjct: 297 EFGVRKEALAFIVAQYPDILGIELRDKLATQQSLFESSILVSSEDFGRVIERMPQAISLG 356

Query: 394 SSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMKHSFQFFQKEMGRPLDDLVAF 453
            +AV  HV+FL SCGF L QV  MV+ CPQLLALN+DIMK SF++FQ EM R L++LV F
Sbjct: 357 RTAVLKHVNFLTSCGFLLSQVSKMVVACPQLLALNMDIMKMSFEYFQNEMERDLEELVEF 416

Query: 454 PAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPSFD 513
           PAFFTYGLEST++PRH  V  KG TCSL+WLLNCSD KF ERM YDTI +EEME   SFD
Sbjct: 417 PAFFTYGLESTVRPRHEMVAKKGFTCSLAWLLNCSDAKFDERMKYDTIGIEEMEVDNSFD 476

Query: 514 MSSLTQPRYDE-SDSDYEEDGEEET 538
            ++L++   DE  D D +ED + ++
Sbjct: 477 TNTLSERVEDEVEDEDLDEDSDYDS 501

BLAST of CmaCh12G010310 vs. ExPASy Swiss-Prot
Match: B6TGN4 (Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MTERF4 PE=2 SV=1)

HSP 1 Score: 557.0 bits (1434), Expect = 2.3e-157
Identity = 270/438 (61.64%), Postives = 352/438 (80.37%), Query Frame = 0

Query: 100 ASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLLKL 159
           +SSLY+RPSL++M+  R   RA V  FL  +G+ P EL GLELPVTVDVM+ER +FL  L
Sbjct: 54  SSSLYARPSLLDMERDRATRRADVDAFLTSLGVDPGELAGLELPVTVDVMRERAEFLGSL 113

Query: 160 GLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVVDLGP 219
           GL  ED+  YPL LGCSV+KNMVPVLDYLGKLGVR+    + LRRYPQVLH+SVVVDL P
Sbjct: 114 GLTQEDLAAYPLALGCSVRKNMVPVLDYLGKLGVRRDALPDLLRRYPQVLHASVVVDLAP 173

Query: 220 VVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPE 279
           VVKYLQGMD++P+D+PRVLERYPE+LGFKLEGTMSTSVAYLVGIGV RR++G V+T++PE
Sbjct: 174 VVKYLQGMDVRPTDVPRVLERYPELLGFKLEGTMSTSVAYLVGIGVGRRQVGSVITRFPE 233

Query: 280 ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVRE 339
           +LGMRV ++IKPFVE+LE +G+ RLA+AR+IEK+P++LGFGL++KVKPNI+ L++  VR+
Sbjct: 234 VLGMRVGKIIKPFVEHLEGIGLQRLAIARIIEKKPYVLGFGLQEKVKPNIEALVDIGVRK 293

Query: 340 AYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIELDNDNFGTIIEKMPQVVSLSSSAVNS 399
             L+SIV QY +++G++L  KL +Q++L +S I +  ++FG ++E+MPQ +SL  +AV  
Sbjct: 294 EALASIVMQYPDVLGLELRDKLVAQQSLFESSILVSREDFGRVLERMPQAISLGRAAVLK 353

Query: 400 HVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMKHSFQFFQKEMGRPLDDLVAFPAFFTY 459
           HV+FL +CGF L QV  MV+ CPQLLALN+DIM+ +F++F+ EM R L++LV FPAFFTY
Sbjct: 354 HVNFLTACGFMLSQVSKMVVACPQLLALNIDIMRMNFEYFKNEMERDLEELVEFPAFFTY 413

Query: 460 GLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPSFDM-SSLT 519
           G+EST++PRH  V  KG+TCSL+WLLNCSD KF ERM YDTI +EEME   S DM +S+ 
Sbjct: 414 GIESTVRPRHEMVSRKGLTCSLAWLLNCSDAKFDERMKYDTIGVEEMEREDSSDMNASVD 473

Query: 520 QPRYDE-SDSDYEEDGEE 536
           +   +E  DSDY +  +E
Sbjct: 474 EVESEEYEDSDYGDSDDE 491

BLAST of CmaCh12G010310 vs. ExPASy Swiss-Prot
Match: F4IHL3 (Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=MTERF2 PE=3 SV=1)

HSP 1 Score: 147.5 bits (371), Expect = 4.3e-34
Identity = 88/360 (24.44%), Postives = 174/360 (48.33%), Query Frame = 0

Query: 178 KKNMVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRV 237
           ++ +  +++YL   GVR+      + R P++L  S + ++   V +   M +  +D   +
Sbjct: 278 REELNEIVEYLESNGVRRDWMGYVVGRCPELLSFS-MEEVKSRVDFFLKMGMNQNDFGTM 337

Query: 238 LERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLE 297
           +  YP+++GF     M   + YL   G++  E+G +L   P ++G  +    KP V+Y  
Sbjct: 338 VYDYPKIIGFFSFQVMEKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFY 397

Query: 298 SLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDL 357
            LGIP+  + R++  +P +    LEK + P ++ L E  +    + +++ ++  ++   L
Sbjct: 398 YLGIPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEAIGNMLVKFPSLLTNSL 457

Query: 358 ETKLPSQRNLLKSIIELDNDNFGTIIEKMPQVVSLS-SSAVNSHVDFLKSCGFSLQQVKN 417
             K+      L +   +   + G +I   P ++  S  + +  ++ +  S G    Q+  
Sbjct: 458 YKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGE 517

Query: 418 MVIGCPQLLALNLDIMKHSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKG 477
           M+   P LL  N+D ++  +++ ++ M RPL DL+ FP FF+Y LE  I PRH  +V   
Sbjct: 518 MIADFPMLLRYNVDNLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVENR 577

Query: 478 ITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEE 537
           +   L ++L C+DE+F  R+       E  E     + S  +     + +  + ++ EEE
Sbjct: 578 VNFKLRYMLACTDEEFERRVRDKVERRERFEAGLDSEDSQPSDENISDQEIAFSDEAEEE 636

BLAST of CmaCh12G010310 vs. ExPASy Swiss-Prot
Match: Q9SZL6 (Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidopsis thaliana OX=3702 GN=MTERF6 PE=2 SV=1)

HSP 1 Score: 137.5 bits (345), Expect = 4.4e-31
Identity = 68/276 (24.64%), Postives = 145/276 (52.54%), Query Frame = 0

Query: 229 IKPSDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARV 288
           I+   +P ++ R P++L  +L+  +   V  L  +G   RE+   +TK+P IL   V   
Sbjct: 54  IQERKLPYIVSRCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEK 113

Query: 289 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNV-REAYLSSIVA 348
           + P + + ++LG+P   + ++I   P ++ + ++ K+   +  L    + ++  +  ++ 
Sbjct: 114 LCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLV 173

Query: 349 QYAEIIGMDLETKLPSQRNLLKSIIELDNDNFGTIIEKMPQVVSLS-SSAVNSHVDFLKS 408
           +   ++G  ++ +L      LKS + L  D   +++   PQ++    +  +  + D+LK 
Sbjct: 174 KNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKE 233

Query: 409 CGFSLQQVKNMVIGCPQLLALNL-DIMKHSFQFFQKEMGRPLDDLVAFPAFFTYGLESTI 468
           CGF   Q+  MV G PQ+L  ++ + ++   +F  + MGR +D++ ++P FF +GL+  +
Sbjct: 234 CGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKV 293

Query: 469 KPRHRKVVHKGITCSLSWLLNCSDEKFAERMTYDTI 502
           + R + V    I CSL  +L+C+ +KF E+  +  +
Sbjct: 294 ESRFKLVKKNNIDCSLREMLDCNTKKFHEKFGFSEV 329

BLAST of CmaCh12G010310 vs. ExPASy TrEMBL
Match: A0A6J1HPD6 (transcription termination factor MTERF4, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111465502 PE=3 SV=1)

HSP 1 Score: 1025.0 bits (2649), Expect = 1.1e-295
Identity = 524/524 (100.00%), Postives = 524/524 (100.00%), Query Frame = 0

Query: 14  MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR 73
           MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR
Sbjct: 1   MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR 60

Query: 74  KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 133
           KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV
Sbjct: 61  KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 120

Query: 134 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 193
           PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 180

Query: 194 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 253
           RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 240

Query: 254 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 313
           STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300

Query: 314 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 373
           PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE
Sbjct: 301 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 360

Query: 374 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 433
           LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK
Sbjct: 361 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 420

Query: 434 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 493
           HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA
Sbjct: 421 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 480

Query: 494 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 538
           ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET
Sbjct: 481 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 524

BLAST of CmaCh12G010310 vs. ExPASy TrEMBL
Match: A0A6J1FDE7 (transcription termination factor MTERF4, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111444359 PE=3 SV=1)

HSP 1 Score: 997.7 bits (2578), Expect = 1.9e-287
Identity = 510/524 (97.33%), Postives = 514/524 (98.09%), Query Frame = 0

Query: 14  MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR 73
           MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKF TTSTFQ SPNHEKR  LITKLQYSRGD 
Sbjct: 1   MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFDTTSTFQTSPNHEKRRGLITKLQYSRGDW 60

Query: 74  KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 133
           +TVSKSLHSTITSKPYSSKV RKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV
Sbjct: 61  RTVSKSLHSTITSKPYSSKVGRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 120

Query: 134 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 193
           PDELDGLELPVTVDVMKERVDFLLKLGL IEDINNYPLVLGCSVKKNMVPVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 180

Query: 194 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 253
           RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 240

Query: 254 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 313
           STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300

Query: 314 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 373
           PHILGFGLEK+VKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE
Sbjct: 301 PHILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 360

Query: 374 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 433
           LDNDNFGTIIEKMPQVVSLSSSAVNSHVD LKSCGFSLQQVKNMVIGCPQLLALNLDIMK
Sbjct: 361 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDCLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 420

Query: 434 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 493
           HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVV KG+TCSLSWLLNCSDEKFA
Sbjct: 421 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCSLSWLLNCSDEKFA 480

Query: 494 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 538
           ERMTYDTIDMEEMETAP+FDMSSLTQPRYDESDSDYEED EEET
Sbjct: 481 ERMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDSEEET 524

BLAST of CmaCh12G010310 vs. ExPASy TrEMBL
Match: A0A6J1DUY1 (transcription termination factor MTERF4, chloroplastic OS=Momordica charantia OX=3673 GN=LOC111023323 PE=3 SV=1)

HSP 1 Score: 914.8 bits (2363), Expect = 1.6e-262
Identity = 469/524 (89.50%), Postives = 491/524 (93.70%), Query Frame = 0

Query: 14  MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR 73
           MMIR H GVVKPNFSI++ +MPTLKC KF F TTS FQ   N ++R  LIT+LQYS GDR
Sbjct: 1   MMIRRHAGVVKPNFSIIVPKMPTLKCCKFTFTTTSIFQTPSNRDRRRGLITRLQYSDGDR 60

Query: 74  KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 133
           +TVSKSLHSTITSKP S++V RKEGSASSLYS PSLVEMKNVR ENRAKVYEFL GIGIV
Sbjct: 61  RTVSKSLHSTITSKPSSTRVGRKEGSASSLYSHPSLVEMKNVRLENRAKVYEFLCGIGIV 120

Query: 134 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 193
           PDELDGLELPVTVDVMKERVDFLLKLGL IEDINNYPLVLGCSVKKNM+PVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180

Query: 194 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 253
           RKSTFTEFLRRYPQVLH+SVVVDL PVVKYLQGMDIKP+DIP VLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLRRYPQVLHASVVVDLAPVVKYLQGMDIKPNDIPHVLERYPEVLGFKLEGTM 240

Query: 254 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 313
           STSVAYLVGIGVAR+EIGGVLTKYP+ILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVARKEIGGVLTKYPDILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300

Query: 314 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 373
           PHILGFGLEKKVKPNIQ+LL+FNVRE YLSSIVAQYAEIIGMDLETKL SQRNLLKSIIE
Sbjct: 301 PHILGFGLEKKVKPNIQMLLKFNVREEYLSSIVAQYAEIIGMDLETKLLSQRNLLKSIIE 360

Query: 374 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 433
           LD+DNFGTII KMPQ+VSLSSSAV +HVDFLKSCGFSL QVKNMVIGCPQLLALNLDIMK
Sbjct: 361 LDDDNFGTIIGKMPQIVSLSSSAVINHVDFLKSCGFSLVQVKNMVIGCPQLLALNLDIMK 420

Query: 434 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 493
           HSFQFFQ EMGRPLDDLV FPAFFTYGLESTIKPRHRKVV KGITCSLSWLLNCSDEKFA
Sbjct: 421 HSFQFFQMEMGRPLDDLVTFPAFFTYGLESTIKPRHRKVVQKGITCSLSWLLNCSDEKFA 480

Query: 494 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 538
           ERMTYDTIDMEEMETAP+FDM+SL QPR D+SDSDYEED EEET
Sbjct: 481 ERMTYDTIDMEEMETAPTFDMNSLIQPRNDDSDSDYEEDSEEET 524

BLAST of CmaCh12G010310 vs. ExPASy TrEMBL
Match: A0A0A0KRV7 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G466880 PE=3 SV=1)

HSP 1 Score: 899.8 bits (2324), Expect = 5.4e-258
Identity = 456/524 (87.02%), Postives = 483/524 (92.18%), Query Frame = 0

Query: 15  MIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDRK 74
           MIRGH GV KPNFS+ML EM TL+CH FKF  T TFQ   NHEKR  LIT+LQYS GDR+
Sbjct: 1   MIRGHAGVAKPNFSLMLFEMSTLECHNFKFTATRTFQMPYNHEKRKGLITQLQYSHGDRR 60

Query: 75  TVSKSLHSTITSKPYSSKVSRKEGS-ASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 134
           TV KSLHSTI+SK YSSKV RKEGS  SSLYSRPSLVEMKNVR ENR+KVYEFLRGIG+V
Sbjct: 61  TVFKSLHSTISSKSYSSKVDRKEGSIGSSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV 120

Query: 135 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 194
           PDELDGLELPVTVDVMKERVDFLLKLGL IEDINNYPL+LGCSVKKNM+PVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLILGCSVKKNMIPVLDYLGKLGV 180

Query: 195 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 254
           RKSTFTEFL+RYPQVLHSSVVVDL PV+KYLQGMDIKP+DIPRVLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLKRYPQVLHSSVVVDLAPVIKYLQGMDIKPNDIPRVLERYPEVLGFKLEGTM 240

Query: 255 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 314
           STSVAYLVGIGV RREIGGVLTKYPEILGMRVARVIKPFVEYLE LGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVTRREIGGVLTKYPEILGMRVARVIKPFVEYLEGLGIPRLAVARLIEKR 300

Query: 315 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 374
           PHILGFGLEKKVKPNIQLLLEF VREAYL SIVAQYAEIIG+DLE  L +QRNLLKS+IE
Sbjct: 301 PHILGFGLEKKVKPNIQLLLEFKVREAYLPSIVAQYAEIIGIDLEANLQTQRNLLKSLIE 360

Query: 375 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 434
           LDNDNFGTIIEKMPQVVSLS SAV +HVDFLK+CGFSL QVKNMVIGCPQLLALN+DIMK
Sbjct: 361 LDNDNFGTIIEKMPQVVSLSRSAVINHVDFLKTCGFSLLQVKNMVIGCPQLLALNIDIMK 420

Query: 435 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 494
           HSF+FFQ EMGRPL+DL  FPAFFTYGLESTIKPRHRKVV KGI+CSLSWLLNC+DEKF 
Sbjct: 421 HSFEFFQMEMGRPLEDLATFPAFFTYGLESTIKPRHRKVVQKGISCSLSWLLNCADEKFM 480

Query: 495 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 538
           ERM+YDTIDMEEMET P+FDM+SL QPR DESDS+YEED EEE+
Sbjct: 481 ERMSYDTIDMEEMETTPTFDMNSLIQPRNDESDSEYEEDSEEES 524

BLAST of CmaCh12G010310 vs. ExPASy TrEMBL
Match: A0A1S3CR33 (transcription termination factor MTERF4, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103503367 PE=3 SV=1)

HSP 1 Score: 897.9 bits (2319), Expect = 2.1e-257
Identity = 457/524 (87.21%), Postives = 482/524 (91.98%), Query Frame = 0

Query: 15  MIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDRK 74
           MIRGH GVVKPN S ML EM TL+CH FKF  T TF  S  HEKR  L T+LQYS GDR+
Sbjct: 1   MIRGHAGVVKPNVSFMLFEMSTLECHNFKFTATPTFLTSCIHEKRRCLTTQLQYSHGDRR 60

Query: 75  TVSKSLHSTITSKPYSSKVSRKEGSA-SSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 134
           TV KSLHSTI+SKPYSSKV RK GS+ SSLYSRPSLVEMKNVR ENR+KVYEFLRGIG+V
Sbjct: 61  TVFKSLHSTISSKPYSSKVGRKGGSSGSSLYSRPSLVEMKNVRLENRSKVYEFLRGIGVV 120

Query: 135 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 194
           PDELDGLELPVTVDVMKERVDFLLKLGL IEDINNYPLVLGCSVKKNM+PVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMIPVLDYLGKLGV 180

Query: 195 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 254
           RKSTFTEFL+RYPQVLHSSVVVDL PVVKYLQGMDIKP+DIPRVLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLKRYPQVLHSSVVVDLAPVVKYLQGMDIKPNDIPRVLERYPEVLGFKLEGTM 240

Query: 255 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 314
           STSVAYLVGIGVARREIGG+LTKYPEILGMRVARVIKPFVEYLE LGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVARREIGGILTKYPEILGMRVARVIKPFVEYLEGLGIPRLAVARLIEKR 300

Query: 315 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 374
           PHILGFGLEKKVKPNIQLLLEF VREAYL SIVAQYAEIIGMDLE  L +QRNLLKS+IE
Sbjct: 301 PHILGFGLEKKVKPNIQLLLEFKVREAYLPSIVAQYAEIIGMDLEANLQTQRNLLKSLIE 360

Query: 375 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 434
           LDNDNFGTIIEKMPQ+VSLS SAV +HVDFLK+CGFSL QVKNMVIGCPQLLALN+DIMK
Sbjct: 361 LDNDNFGTIIEKMPQIVSLSRSAVINHVDFLKTCGFSLLQVKNMVIGCPQLLALNIDIMK 420

Query: 435 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 494
           HSF+FFQ EMGRPL+DL  FPAFFTYGLESTIKPRHRKVV KGI+CSLSWLLNC+DEKF 
Sbjct: 421 HSFEFFQMEMGRPLEDLATFPAFFTYGLESTIKPRHRKVVQKGISCSLSWLLNCADEKFV 480

Query: 495 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 538
           ERM+YDTIDMEEMET P+FDM+SL QPR DESDS+YEED EEET
Sbjct: 481 ERMSYDTIDMEEMETTPTFDMNSLVQPRNDESDSEYEEDSEEET 524

BLAST of CmaCh12G010310 vs. NCBI nr
Match: XP_022965673.1 (transcription termination factor MTERF4, chloroplastic [Cucurbita maxima] >XP_022965674.1 transcription termination factor MTERF4, chloroplastic [Cucurbita maxima] >XP_022965675.1 transcription termination factor MTERF4, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1025.0 bits (2649), Expect = 2.3e-295
Identity = 524/524 (100.00%), Postives = 524/524 (100.00%), Query Frame = 0

Query: 14  MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR 73
           MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR
Sbjct: 1   MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR 60

Query: 74  KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 133
           KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV
Sbjct: 61  KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 120

Query: 134 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 193
           PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 180

Query: 194 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 253
           RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 240

Query: 254 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 313
           STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300

Query: 314 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 373
           PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE
Sbjct: 301 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 360

Query: 374 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 433
           LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK
Sbjct: 361 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 420

Query: 434 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 493
           HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA
Sbjct: 421 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 480

Query: 494 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 538
           ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET
Sbjct: 481 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 524

BLAST of CmaCh12G010310 vs. NCBI nr
Match: KAG7021053.1 (Transcription termination factor MTERF4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1020.8 bits (2638), Expect = 4.4e-294
Identity = 519/537 (96.65%), Postives = 528/537 (98.32%), Query Frame = 0

Query: 1   MQFFQVQGAKPGVMMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRG 60
           M+ + VQGAKPGVMMIRGHTGV+KPNFSIMLLEMPTLKCHKFKFGTTSTFQ SPNHEKR 
Sbjct: 1   MEVWMVQGAKPGVMMIRGHTGVLKPNFSIMLLEMPTLKCHKFKFGTTSTFQTSPNHEKRR 60

Query: 61  SLITKLQYSRGDRKTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENR 120
            LITKLQYSRGDR+TVSKSLHSTITSKPYSSKV RKEGSASSLYSRPSLVEMKNVRQENR
Sbjct: 61  GLITKLQYSRGDRRTVSKSLHSTITSKPYSSKVGRKEGSASSLYSRPSLVEMKNVRQENR 120

Query: 121 AKVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKN 180
           AKVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLLKLGL IEDINNYPLVLGCSVKKN
Sbjct: 121 AKVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKN 180

Query: 181 MVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLER 240
           MVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLER
Sbjct: 181 MVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLER 240

Query: 241 YPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLG 300
           YPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLG
Sbjct: 241 YPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLG 300

Query: 301 IPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETK 360
           IPRLAVARLIEKRPHILGFGLEK+VKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETK
Sbjct: 301 IPRLAVARLIEKRPHILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETK 360

Query: 361 LPSQRNLLKSIIELDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIG 420
           LPSQRNLLKSIIELDN+NFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIG
Sbjct: 361 LPSQRNLLKSIIELDNNNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIG 420

Query: 421 CPQLLALNLDIMKHSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCS 480
           CPQLLALNLDIMKHSFQFF+KEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVV KG+TCS
Sbjct: 421 CPQLLALNLDIMKHSFQFFRKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCS 480

Query: 481 LSWLLNCSDEKFAERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 538
           LSWLLNCSDEKFAERMTYDTIDMEEMETAP+FDMSSLTQPRYDESDSDYEED EEET
Sbjct: 481 LSWLLNCSDEKFAERMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDSEEET 537

BLAST of CmaCh12G010310 vs. NCBI nr
Match: KAG6586221.1 (Transcription termination factor MTERF4, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.6e-288
Identity = 510/524 (97.33%), Postives = 517/524 (98.66%), Query Frame = 0

Query: 14  MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR 73
           MMIRGHTGV+KPNFSIMLLEMPTLKCHKFKFGTTSTFQ SPNHEKR  LITKLQYSRGDR
Sbjct: 1   MMIRGHTGVLKPNFSIMLLEMPTLKCHKFKFGTTSTFQTSPNHEKRRGLITKLQYSRGDR 60

Query: 74  KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 133
           +TVSKSLHSTITSKPYSSKV RKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV
Sbjct: 61  RTVSKSLHSTITSKPYSSKVGRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 120

Query: 134 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 193
           PDELDGLELPVTVDVMKERVDFLLKLGL IEDINNYPLVLGCSVKKNMVPVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 180

Query: 194 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 253
           RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 240

Query: 254 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 313
           STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300

Query: 314 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 373
           PHILGFGLEK+VKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE
Sbjct: 301 PHILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 360

Query: 374 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 433
           LDN+NFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK
Sbjct: 361 LDNNNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 420

Query: 434 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 493
           HSFQFF+KEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVV KG+TCSLSWLLNCSDEKFA
Sbjct: 421 HSFQFFRKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGMTCSLSWLLNCSDEKFA 480

Query: 494 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 538
           ERMTYDTIDMEEMETAP+FDMSSLTQPRYDESDSDYEED EEET
Sbjct: 481 ERMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDSEEET 524

BLAST of CmaCh12G010310 vs. NCBI nr
Match: XP_023538106.1 (transcription termination factor MTERF4, chloroplastic [Cucurbita pepo subsp. pepo] >XP_023538107.1 transcription termination factor MTERF4, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1001.1 bits (2587), Expect = 3.6e-288
Identity = 512/524 (97.71%), Postives = 516/524 (98.47%), Query Frame = 0

Query: 14  MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR 73
           MMIRGHTGVVKPNFSIMLLEMPTLKC KFKF TTSTFQ SPNHEKR  LITKLQYSRGDR
Sbjct: 1   MMIRGHTGVVKPNFSIMLLEMPTLKCQKFKFITTSTFQTSPNHEKRRGLITKLQYSRGDR 60

Query: 74  KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 133
           +TVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV
Sbjct: 61  RTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 120

Query: 134 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 193
           PDELDGLELPVTVDVMKERVDFLLKLGL IEDINNYPLVLGCSVKKNMVPVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 180

Query: 194 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 253
           RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 240

Query: 254 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 313
           STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300

Query: 314 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 373
           PHILGFGLEK+VKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE
Sbjct: 301 PHILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 360

Query: 374 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 433
           LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK
Sbjct: 361 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 420

Query: 434 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 493
           HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVV KG+TCSLSWLLNCSDEKFA
Sbjct: 421 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCSLSWLLNCSDEKFA 480

Query: 494 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 538
           ERMTYDTIDMEEMETAP+FDMSSLTQPRYDESDSDYEED EEET
Sbjct: 481 ERMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDSEEET 524

BLAST of CmaCh12G010310 vs. NCBI nr
Match: XP_022938209.1 (transcription termination factor MTERF4, chloroplastic [Cucurbita moschata] >XP_022938210.1 transcription termination factor MTERF4, chloroplastic [Cucurbita moschata] >XP_022938211.1 transcription termination factor MTERF4, chloroplastic [Cucurbita moschata])

HSP 1 Score: 997.7 bits (2578), Expect = 4.0e-287
Identity = 510/524 (97.33%), Postives = 514/524 (98.09%), Query Frame = 0

Query: 14  MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKRGSLITKLQYSRGDR 73
           MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKF TTSTFQ SPNHEKR  LITKLQYSRGD 
Sbjct: 1   MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFDTTSTFQTSPNHEKRRGLITKLQYSRGDW 60

Query: 74  KTVSKSLHSTITSKPYSSKVSRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 133
           +TVSKSLHSTITSKPYSSKV RKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV
Sbjct: 61  RTVSKSLHSTITSKPYSSKVGRKEGSASSLYSRPSLVEMKNVRQENRAKVYEFLRGIGIV 120

Query: 134 PDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 193
           PDELDGLELPVTVDVMKERVDFLLKLGL IEDINNYPLVLGCSVKKNMVPVLDYLGKLGV
Sbjct: 121 PDELDGLELPVTVDVMKERVDFLLKLGLTIEDINNYPLVLGCSVKKNMVPVLDYLGKLGV 180

Query: 194 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 253
           RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM
Sbjct: 181 RKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYPEVLGFKLEGTM 240

Query: 254 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 313
           STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR
Sbjct: 241 STSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIPRLAVARLIEKR 300

Query: 314 PHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 373
           PHILGFGLEK+VKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE
Sbjct: 301 PHILGFGLEKRVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLPSQRNLLKSIIE 360

Query: 374 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 433
           LDNDNFGTIIEKMPQVVSLSSSAVNSHVD LKSCGFSLQQVKNMVIGCPQLLALNLDIMK
Sbjct: 361 LDNDNFGTIIEKMPQVVSLSSSAVNSHVDCLKSCGFSLQQVKNMVIGCPQLLALNLDIMK 420

Query: 434 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLSWLLNCSDEKFA 493
           HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVV KG+TCSLSWLLNCSDEKFA
Sbjct: 421 HSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVQKGVTCSLSWLLNCSDEKFA 480

Query: 494 ERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEET 538
           ERMTYDTIDMEEMETAP+FDMSSLTQPRYDESDSDYEED EEET
Sbjct: 481 ERMTYDTIDMEEMETAPTFDMSSLTQPRYDESDSDYEEDSEEET 524

BLAST of CmaCh12G010310 vs. TAIR 10
Match: AT4G02990.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 684.9 bits (1766), Expect = 5.3e-197
Identity = 360/536 (67.16%), Postives = 425/536 (79.29%), Query Frame = 0

Query: 14  MMIRGHTGVVKPNFSIMLLEMPTLKCHKFKFGTTSTFQASPNHEKR--GSLITKLQYSRG 73
           M IR   G  KP F ++  E P+    + +  + ST+    NH+KR    +   +Q +  
Sbjct: 1   MKIRFCNGFTKPGFLLVHFEPPSFFAVRSRSLSDSTYGNLCNHKKRPGTGIGLTVQCAIA 60

Query: 74  DRKTVSKSLHSTITSKPYSSKVSR---------KEGSASSLYSRPSLVEMKNVRQENRAK 133
           +R+  S+SL S    +   S  S          K   + SLYSRPSL++M   +  NRAK
Sbjct: 61  NRRFSSRSLDSPRRERSSRSSSSSGRDRDRDKDKGRDSKSLYSRPSLLDMNKEKAANRAK 120

Query: 134 VYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLLKLGLAIEDINNYPLVLGCSVKKNMV 193
           VYEFLRGIGIVPDELDGLELPVT DVMKERV+FL KLGL IEDINNYPLVLGCSVKKNMV
Sbjct: 121 VYEFLRGIGIVPDELDGLELPVTADVMKERVEFLHKLGLTIEDINNYPLVLGCSVKKNMV 180

Query: 194 PVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRVLERYP 253
           PVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVV+DL PVVKYLQG+DIKPSD+PRVLERYP
Sbjct: 181 PVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVIDLAPVVKYLQGLDIKPSDVPRVLERYP 240

Query: 254 EVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLESLGIP 313
           EVLGFKLEGTMSTSVAYLVGIGVARREIGG+LT+YPEILGMRVAR+IKP VEYLE LGIP
Sbjct: 241 EVLGFKLEGTMSTSVAYLVGIGVARREIGGILTRYPEILGMRVARIIKPLVEYLEVLGIP 300

Query: 314 RLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDLETKLP 373
           RLA ARLIEKRPHILGF L+  VKPN+Q+L +FNVRE  L SI+AQY EIIG+DL+ KL 
Sbjct: 301 RLAAARLIEKRPHILGFELDDTVKPNVQILQDFNVRETSLPSIIAQYPEIIGIDLKPKLD 360

Query: 374 SQRNLLKSIIELDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCGFSLQQVKNMVIGCP 433
           +QR LL S I L+ ++ G++IE+MPQ VSLS S +  H+DFL  CGFS+ Q + MVIGCP
Sbjct: 361 TQRKLLCSAIHLNPEDLGSLIERMPQFVSLSESPMLKHIDFLTKCGFSIDQTREMVIGCP 420

Query: 434 QLLALNLDIMKHSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKGITCSLS 493
           Q+LALNL IMK SF++FQKEM RPL DLV FPAFFTYGLEST+KPRH+K++ KGI CSL+
Sbjct: 421 QVLALNLGIMKLSFEYFQKEMKRPLQDLVDFPAFFTYGLESTVKPRHKKIIKKGIKCSLA 480

Query: 494 WLLNCSDEKFAERMTYDTIDMEEMETAP-SFDMSSLTQP-RYDESDSDYEEDGEEE 537
           W+LNCSDEKF +RM+YDTID+EE+ET P SFDM++L QP R +ESDS+YEE+ +++
Sbjct: 481 WMLNCSDEKFEQRMSYDTIDIEEVETDPSSFDMNTLMQPEREEESDSEYEEEEDDD 536

BLAST of CmaCh12G010310 vs. TAIR 10
Match: AT2G44020.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 440.3 bits (1131), Expect = 2.3e-123
Identity = 206/428 (48.13%), Postives = 313/428 (73.13%), Query Frame = 0

Query: 109 LVEMKNVRQENRAKVYEFLRGIGIVPDELDGLELPVTVDVMKERVDFLLKLGLAIEDINN 168
           +++ K  +  NR K+ ++L+G+GI+ DEL+ +ELP T++VM ERV+FL KLGL I+DIN 
Sbjct: 72  VIQGKKEKLVNRVKICDYLKGLGIITDELESIELPSTIEVMCERVEFLQKLGLTIDDINE 131

Query: 169 YPLVLGCSVKKNMVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMD 228
           YPL+LGCSV+KN++PVL YL K+G+ +S   EF++ YPQVLH+SVVV+L PVVK+L+G+D
Sbjct: 132 YPLMLGCSVRKNLIPVLAYLEKIGISRSKLGEFVKNYPQVLHASVVVELAPVVKFLRGLD 191

Query: 229 IKPSDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARV 288
           ++  D+  VL +YPE+LGFKLEGTMSTSVAYLV IGV+ R+IG ++T+YP +LGMRV  +
Sbjct: 192 VEKQDLGYVLMKYPELLGFKLEGTMSTSVAYLVSIGVSPRDIGPMVTQYPYLLGMRVGTM 251

Query: 289 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQ 348
           IKP V+YL S+G+P+  VAR++EKR +I+G+ LE+ VKPN+  L+ F V++  L  ++AQ
Sbjct: 252 IKPLVDYLISIGLPKKIVARMLEKRSYIVGYNLEETVKPNVDCLISFGVKKELLPLLIAQ 311

Query: 349 YAEIIGMDLETKLPSQRNLLKSIIELDNDNFGTIIEKMPQVVSLSSSAVNSHVDFLKSCG 408
           Y +I+G+ ++ K+ +Q+      +++D + F  ++EKMPQ+VSL  + +   ++FL    
Sbjct: 312 YPQILGLPVKAKMSTQQYFFSLKLKIDPEGFARVVEKMPQIVSLKQNVIMKPIEFLLGRA 371

Query: 409 FSLQQVKNMVIGCPQLLALNLDIMKHSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPR 468
           F ++ +  MV+ CPQ+L   +++MK+S+ F++ EMGRP+ +LV +P +FTY LES IKPR
Sbjct: 372 FQVEDIAKMVVRCPQILCSRVELMKNSYYFYKTEMGRPMKELVEYPEYFTYSLESRIKPR 431

Query: 469 HRKVVHKGITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSD 528
           ++K+  KGI  SL+W LNCSD++F ER+  + ID +     P+FDM    +    E  +D
Sbjct: 432 YQKLQSKGIRSSLNWFLNCSDQRFEERLQGNFIDPD--TEGPTFDMGGKLEMPGGEIVTD 491

Query: 529 YEEDGEEE 537
            EED  ++
Sbjct: 492 EEEDESDD 497

BLAST of CmaCh12G010310 vs. TAIR 10
Match: AT2G21710.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 147.5 bits (371), Expect = 3.0e-35
Identity = 88/360 (24.44%), Postives = 174/360 (48.33%), Query Frame = 0

Query: 178 KKNMVPVLDYLGKLGVRKSTFTEFLRRYPQVLHSSVVVDLGPVVKYLQGMDIKPSDIPRV 237
           ++ +  +++YL   GVR+      + R P++L  S + ++   V +   M +  +D   +
Sbjct: 278 REELNEIVEYLESNGVRRDWMGYVVGRCPELLSFS-MEEVKSRVDFFLKMGMNQNDFGTM 337

Query: 238 LERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARVIKPFVEYLE 297
           +  YP+++GF     M   + YL   G++  E+G +L   P ++G  +    KP V+Y  
Sbjct: 338 VYDYPKIIGFFSFQVMEKKINYLKEFGLSTEEVGRLLAYKPHLMGCSIEERWKPLVKYFY 397

Query: 298 SLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNVREAYLSSIVAQYAEIIGMDL 357
            LGIP+  + R++  +P +    LEK + P ++ L E  +    + +++ ++  ++   L
Sbjct: 398 YLGIPKEGMKRILVVKPILYCIDLEKTIAPKVRFLQEMGIPNEAIGNMLVKFPSLLTNSL 457

Query: 358 ETKLPSQRNLLKSIIELDNDNFGTIIEKMPQVVSLS-SSAVNSHVDFLKSCGFSLQQVKN 417
             K+      L +   +   + G +I   P ++  S  + +  ++ +  S G    Q+  
Sbjct: 458 YKKIRPVVIFLLTRAGVTQKDIGKVIAMDPALLGCSIGTKLEPNMRYYISLGIRFYQLGE 517

Query: 418 MVIGCPQLLALNLDIMKHSFQFFQKEMGRPLDDLVAFPAFFTYGLESTIKPRHRKVVHKG 477
           M+   P LL  N+D ++  +++ ++ M RPL DL+ FP FF+Y LE  I PRH  +V   
Sbjct: 518 MIADFPMLLRYNVDNLRPKYRYLRRTMIRPLQDLIEFPRFFSYSLERRIIPRHTIMVENR 577

Query: 478 ITCSLSWLLNCSDEKFAERMTYDTIDMEEMETAPSFDMSSLTQPRYDESDSDYEEDGEEE 537
           +   L ++L C+DE+F  R+       E  E     + S  +     + +  + ++ EEE
Sbjct: 578 VNFKLRYMLACTDEEFERRVRDKVERRERFEAGLDSEDSQPSDENISDQEIAFSDEAEEE 636

BLAST of CmaCh12G010310 vs. TAIR 10
Match: AT4G38160.1 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 137.5 bits (345), Expect = 3.2e-32
Identity = 68/276 (24.64%), Postives = 145/276 (52.54%), Query Frame = 0

Query: 229 IKPSDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARV 288
           I+   +P ++ R P++L  +L+  +   V  L  +G   RE+   +TK+P IL   V   
Sbjct: 54  IQERKLPYIVSRCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEK 113

Query: 289 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNV-REAYLSSIVA 348
           + P + + ++LG+P   + ++I   P ++ + ++ K+   +  L    + ++  +  ++ 
Sbjct: 114 LCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLV 173

Query: 349 QYAEIIGMDLETKLPSQRNLLKSIIELDNDNFGTIIEKMPQVVSLS-SSAVNSHVDFLKS 408
           +   ++G  ++ +L      LKS + L  D   +++   PQ++    +  +  + D+LK 
Sbjct: 174 KNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKE 233

Query: 409 CGFSLQQVKNMVIGCPQLLALNL-DIMKHSFQFFQKEMGRPLDDLVAFPAFFTYGLESTI 468
           CGF   Q+  MV G PQ+L  ++ + ++   +F  + MGR +D++ ++P FF +GL+  +
Sbjct: 234 CGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKV 293

Query: 469 KPRHRKVVHKGITCSLSWLLNCSDEKFAERMTYDTI 502
           + R + V    I CSL  +L+C+ +KF E+  +  +
Sbjct: 294 ESRFKLVKKNNIDCSLREMLDCNTKKFHEKFGFSEV 329

BLAST of CmaCh12G010310 vs. TAIR 10
Match: AT4G38160.2 (Mitochondrial transcription termination factor family protein )

HSP 1 Score: 137.5 bits (345), Expect = 3.2e-32
Identity = 68/276 (24.64%), Postives = 145/276 (52.54%), Query Frame = 0

Query: 229 IKPSDIPRVLERYPEVLGFKLEGTMSTSVAYLVGIGVARREIGGVLTKYPEILGMRVARV 288
           I+   +P ++ R P++L  +L+  +   V  L  +G   RE+   +TK+P IL   V   
Sbjct: 54  IQERKLPYIVSRCPKILTLRLDERLIPMVECLSSLGRNPREVASAITKFPPILSHSVEEK 113

Query: 289 IKPFVEYLESLGIPRLAVARLIEKRPHILGFGLEKKVKPNIQLLLEFNV-REAYLSSIVA 348
           + P + + ++LG+P   + ++I   P ++ + ++ K+   +  L    + ++  +  ++ 
Sbjct: 114 LCPLLAFFQALGVPETQLGKMILFNPRLISYSIDTKLTVIVSFLASLGLDQDGMIGKVLV 173

Query: 349 QYAEIIGMDLETKLPSQRNLLKSIIELDNDNFGTIIEKMPQVVSLS-SSAVNSHVDFLKS 408
           +   ++G  ++ +L      LKS + L  D   +++   PQ++    +  +  + D+LK 
Sbjct: 174 KNPFLMGYSVDKRLRPTTEFLKSSVGLSEDGIKSVVMNFPQLLCRDVNKILKPNYDYLKE 233

Query: 409 CGFSLQQVKNMVIGCPQLLALNL-DIMKHSFQFFQKEMGRPLDDLVAFPAFFTYGLESTI 468
           CGF   Q+  MV G PQ+L  ++ + ++   +F  + MGR +D++ ++P FF +GL+  +
Sbjct: 234 CGFGDSQIATMVTGYPQILIKSVKNSLQPRIRFLVQVMGRGMDEVASYPEFFHHGLKKKV 293

Query: 469 KPRHRKVVHKGITCSLSWLLNCSDEKFAERMTYDTI 502
           + R + V    I CSL  +L+C+ +KF E+  +  +
Sbjct: 294 ESRFKLVKKNNIDCSLREMLDCNTKKFHEKFGFSEV 329

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9ZT967.5e-19667.16Transcription termination factor MTERF4, chloroplastic OS=Arabidopsis thaliana O... [more]
Q6AUK61.6e-16163.15Transcription termination factor MTERF4, chloroplastic OS=Oryza sativa subsp. ja... [more]
B6TGN42.3e-15761.64Transcription termination factor MTERF4, chloroplastic OS=Zea mays OX=4577 GN=MT... [more]
F4IHL34.3e-3424.44Transcription termination factor MTERF2, chloroplastic OS=Arabidopsis thaliana O... [more]
Q9SZL64.4e-3124.64Transcription termination factor MTERF6, chloroplastic/mitochondrial OS=Arabidop... [more]
Match NameE-valueIdentityDescription
A0A6J1HPD61.1e-295100.00transcription termination factor MTERF4, chloroplastic OS=Cucurbita maxima OX=36... [more]
A0A6J1FDE71.9e-28797.33transcription termination factor MTERF4, chloroplastic OS=Cucurbita moschata OX=... [more]
A0A6J1DUY11.6e-26289.50transcription termination factor MTERF4, chloroplastic OS=Momordica charantia OX... [more]
A0A0A0KRV75.4e-25887.02Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_5G466880 PE=3 SV=1[more]
A0A1S3CR332.1e-25787.21transcription termination factor MTERF4, chloroplastic OS=Cucumis melo OX=3656 G... [more]
Match NameE-valueIdentityDescription
XP_022965673.12.3e-295100.00transcription termination factor MTERF4, chloroplastic [Cucurbita maxima] >XP_02... [more]
KAG7021053.14.4e-29496.65Transcription termination factor MTERF4, chloroplastic, partial [Cucurbita argyr... [more]
KAG6586221.13.6e-28897.33Transcription termination factor MTERF4, chloroplastic, partial [Cucurbita argyr... [more]
XP_023538106.13.6e-28897.71transcription termination factor MTERF4, chloroplastic [Cucurbita pepo subsp. pe... [more]
XP_022938209.14.0e-28797.33transcription termination factor MTERF4, chloroplastic [Cucurbita moschata] >XP_... [more]
Match NameE-valueIdentityDescription
AT4G02990.15.3e-19767.16Mitochondrial transcription termination factor family protein [more]
AT2G44020.12.3e-12348.13Mitochondrial transcription termination factor family protein [more]
AT2G21710.13.0e-3524.44Mitochondrial transcription termination factor family protein [more]
AT4G38160.13.2e-3224.64Mitochondrial transcription termination factor family protein [more]
AT4G38160.23.2e-3224.64Mitochondrial transcription termination factor family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003690Transcription termination factor, mitochondrial/chloroplasticSMARTSM00733mt_12coord: 163..194
e-value: 0.0061
score: 25.8
coord: 271..302
e-value: 1.1E-4
score: 31.6
coord: 199..230
e-value: 0.0014
score: 27.9
coord: 100..133
e-value: 1900.0
score: 1.7
coord: 235..266
e-value: 0.0053
score: 26.0
coord: 415..446
e-value: 0.057
score: 22.5
coord: 343..375
e-value: 700.0
score: 5.1
coord: 380..410
e-value: 0.016
score: 24.4
coord: 307..338
e-value: 3.6E-4
score: 29.9
coord: 447..478
e-value: 0.023
score: 23.9
IPR003690Transcription termination factor, mitochondrial/chloroplasticPFAMPF02536mTERFcoord: 185..493
e-value: 3.6E-105
score: 351.4
IPR003690Transcription termination factor, mitochondrial/chloroplasticPANTHERPTHR13068CGI-12 PROTEIN-RELATEDcoord: 47..526
IPR038538MTERF superfamily, mitochondrial/chloroplasticGENE3D1.25.70.10Transcription termination factor 3, mitochondrialcoord: 149..288
e-value: 1.7E-24
score: 88.4
coord: 289..503
e-value: 2.2E-50
score: 173.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 506..537
NoneNo IPR availablePANTHERPTHR13068:SF109TRANSCRIPTION TERMINATION FACTOR MTERF4, CHLOROPLASTICcoord: 47..526

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G010310.1CmaCh12G010310.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006353 DNA-templated transcription, termination
biological_process GO:0009793 embryo development ending in seed dormancy
biological_process GO:0042794 plastid rRNA transcription
biological_process GO:0006355 regulation of transcription, DNA-templated
biological_process GO:0008380 RNA splicing
cellular_component GO:0009536 plastid
molecular_function GO:0003690 double-stranded DNA binding
molecular_function GO:0003729 mRNA binding