CmaCh12G010280 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G010280
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	nicotianamine synthase
Location	Cma_Chr12: 8172889 .. 8173737 (-)
RNA-Seq Expression	CmaCh12G010280
Synteny	CmaCh12G010280

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTGTTGCGACGGCGAAGGGCTCCTTTTGGTCCAAAAGGTCTCAGAACTTTACCACAAAATCTCCACTCTCCAAAGCCTCCAACCCTCCAAACATGTCAACTCCCTCTTCTCCCAGCTCGTCCTCACCTGCACCCCTCCGCCGCCTCCTCCACCGTCTCCGCCGCTCATCGACATCTCCAAGCTCAGCTCGACGGTCCAAACCATGAGATCCCATCTCATCAAGCTCTGCGGCCAAGCCGAGGGCTTACTCGAGCACCATTACTCGACTATCTTAGCCTCCCACCAGAACCCTCTTGACCATCTCTCCCTTTTCCCTTACTACTCCAACTACCTCAAACTAACCCATCTCGAATTCTCCATTCTGACTCGTTACATATCCCCTGCCCTGCCGTCTAAAGTGGCATTCATCGGCTCAGGTGCGCTCCCATTCTCGTCCATCATCATGGCTTTGAAATACCTCCCCACGACCGAGTTTCACAATTTTGATGTTGACTCATCCGCTAATGCCAAGGCTACAATGCTCGTGGCAAGTGACGTGGACTTGTCGAGGCGGATGACATTCCACACTACCGATATCATGGACGTCACCTCGGATATGTTGAGAGAGTTCGAGGTGGTGTTTTTGGCAGCGTTGGTGGGCATGGAGAAAGAGGAGAAAGTTAAGGTAATCGAGCATTTACGAAAGAACATGACTAGTGGCTCGTTGTTGATGTTGAGAAGTGCGTATGGTGCACGGGCATTTCTTTATCCAGTGGTGGAAGCTTCTGATCTTCGAGGGTTTGAGATTCTTACGACGTTTCATCCTACTGATGATGTTGTCAATTCGATCGTGTTGGCTCGATTATAA

mRNA sequence

ATGTGTTGCGACGGCGAAGGGCTCCTTTTGGTCCAAAAGGTCTCAGAACTTTACCACAAAATCTCCACTCTCCAAAGCCTCCAACCCTCCAAACATGTCAACTCCCTCTTCTCCCAGCTCGTCCTCACCTGCACCCCTCCGCCGCCTCCTCCACCGTCTCCGCCGCTCATCGACATCTCCAAGCTCAGCTCGACGGTCCAAACCATGAGATCCCATCTCATCAAGCTCTGCGGCCAAGCCGAGGGCTTACTCGAGCACCATTACTCGACTATCTTAGCCTCCCACCAGAACCCTCTTGACCATCTCTCCCTTTTCCCTTACTACTCCAACTACCTCAAACTAACCCATCTCGAATTCTCCATTCTGACTCGTTACATATCCCCTGCCCTGCCGTCTAAAGTGGCATTCATCGGCTCAGGTGCGCTCCCATTCTCGTCCATCATCATGGCTTTGAAATACCTCCCCACGACCGAGTTTCACAATTTTGATGTTGACTCATCCGCTAATGCCAAGGCTACAATGCTCGTGGCAAGTGACGTGGACTTGTCGAGGCGGATGACATTCCACACTACCGATATCATGGACGTCACCTCGGATATGTTGAGAGAGTTCGAGGTGGTGTTTTTGGCAGCGTTGGTGGGCATGGAGAAAGAGGAGAAAGTTAAGGTAATCGAGCATTTACGAAAGAACATGACTAGTGGCTCGTTGTTGATGTTGAGAAGTGCGTATGGTGCACGGGCATTTCTTTATCCAGTGGTGGAAGCTTCTGATCTTCGAGGGTTTGAGATTCTTACGACGTTTCATCCTACTGATGATGTTGTCAATTCGATCGTGTTGGCTCGATTATAA

Coding sequence (CDS)

ATGTGTTGCGACGGCGAAGGGCTCCTTTTGGTCCAAAAGGTCTCAGAACTTTACCACAAAATCTCCACTCTCCAAAGCCTCCAACCCTCCAAACATGTCAACTCCCTCTTCTCCCAGCTCGTCCTCACCTGCACCCCTCCGCCGCCTCCTCCACCGTCTCCGCCGCTCATCGACATCTCCAAGCTCAGCTCGACGGTCCAAACCATGAGATCCCATCTCATCAAGCTCTGCGGCCAAGCCGAGGGCTTACTCGAGCACCATTACTCGACTATCTTAGCCTCCCACCAGAACCCTCTTGACCATCTCTCCCTTTTCCCTTACTACTCCAACTACCTCAAACTAACCCATCTCGAATTCTCCATTCTGACTCGTTACATATCCCCTGCCCTGCCGTCTAAAGTGGCATTCATCGGCTCAGGTGCGCTCCCATTCTCGTCCATCATCATGGCTTTGAAATACCTCCCCACGACCGAGTTTCACAATTTTGATGTTGACTCATCCGCTAATGCCAAGGCTACAATGCTCGTGGCAAGTGACGTGGACTTGTCGAGGCGGATGACATTCCACACTACCGATATCATGGACGTCACCTCGGATATGTTGAGAGAGTTCGAGGTGGTGTTTTTGGCAGCGTTGGTGGGCATGGAGAAAGAGGAGAAAGTTAAGGTAATCGAGCATTTACGAAAGAACATGACTAGTGGCTCGTTGTTGATGTTGAGAAGTGCGTATGGTGCACGGGCATTTCTTTATCCAGTGGTGGAAGCTTCTGATCTTCGAGGGTTTGAGATTCTTACGACGTTTCATCCTACTGATGATGTTGTCAATTCGATCGTGTTGGCTCGATTATAA

Protein sequence

MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDVDLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL

Homology

[Close]

BLAST of CmaCh12G010280 vs. ExPASy Swiss-Prot
Match: Q9XGI7 (Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1)

HSP 1 Score: 322.8 bits (826), Expect = 3.9e-87
Identity = 162/272 (59.56%), Postives = 217/272 (79.78%), Query Frame = 0


Query: 10  LVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTM 69
           +V+KV ELY +IS L++L PSK VN LF+ LV TC PP P       ID+SKL   +Q +
Sbjct: 9   VVEKVCELYEQISRLENLSPSKDVNVLFTDLVHTCMPPNP-------IDVSKLCQKIQEI 68

Query: 70  RSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFSILTRYISPA 129
           RSHLIKLCGQAEGLLE H+S IL+S++NPL HL +FPY+ NY+KL+ LE++ILT+  +  
Sbjct: 69  RSHLIKLCGQAEGLLESHFSKILSSYENPLQHLHIFPYFDNYIKLSLLEYNILTKN-TTN 128

Query: 130 LPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDVDLSRRMTFH 189
           +P K+AFIGSG LP +S+++A K+L TT FHN+D+D  AN  A+ LVA+D D+S RMTFH
Sbjct: 129 IPKKIAFIGSGPLPLTSLVLATKHLKTTCFHNYDIDVDANFMASALVAADPDMSSRMTFH 188

Query: 190 TTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAFL 249
           T D+MDVT   L++++VVFLAALVGM+KE+KVKV++HL K M+ G+ LMLRSA+GARAFL
Sbjct: 189 TADVMDVTC-ALKDYDVVFLAALVGMDKEDKVKVVDHLAKYMSPGATLMLRSAHGARAFL 248

Query: 250 YPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           YPV++  DLRGFE+L+ +HPTD+V+NS+++AR
Sbjct: 249 YPVLDPRDLRGFEVLSVYHPTDEVINSVIIAR 271

[Close]

BLAST of CmaCh12G010280 vs. ExPASy Swiss-Prot
Match: Q9C7X5 (Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 SV=1)

HSP 1 Score: 317.0 bits (811), Expect = 2.1e-85
Identity = 162/273 (59.34%), Postives = 215/273 (78.75%), Query Frame = 0


Query: 10  LVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTM 69
           LV K+ +LY KIS L++L+P + V++LF QLV TC PP P       ID++K+S  +Q M
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPN------IDVTKMSENIQEM 68

Query: 70  RSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILTRYISP 129
           RS+LIK+CG+AEG LEHH+S+IL S + NPL HL+LFPYY+NYLKL+ LEF +L + ++ 
Sbjct: 69  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 128

Query: 130 ALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDVDLSRRMTF 189
            +P  VAFIGSG LP +S+++A  +L  + FHNFD+D SAN  A  LV+SD DLS+RM F
Sbjct: 129 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 188

Query: 190 HTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAF 249
           HT DIMDVT + L+ F+VVFLAALVGM+K+EKVKV+EHL K+M+ G+LLMLRSA+G RAF
Sbjct: 189 HTVDIMDVT-ESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAF 248

Query: 250 LYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LYP+VE  DL GFE+L+ +HPTD+V+NSIV++R
Sbjct: 249 LYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of CmaCh12G010280 vs. ExPASy Swiss-Prot
Match: O80483 (Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1)

HSP 1 Score: 313.9 bits (803), Expect = 1.8e-84
Identity = 163/273 (59.71%), Postives = 215/273 (78.75%), Query Frame = 0


Query: 10  LVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTM 69
           LVQ + +LY KIS L+SL+PS+ VN LF QLV TC PP P       ID++K+   VQ +
Sbjct: 8   LVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPN------IDVTKMCDRVQEI 67

Query: 70  RSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILTRYISP 129
           R +LIK+CG AEG LE+H+S+IL S+Q NPL HL++FPYY+NYLKL  LEF +L + ++ 
Sbjct: 68  RLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNG 127

Query: 130 ALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDVDLSRRMTF 189
            +P  VAFIGSG LP +SI++A  +L  T FHNFD+D SAN+ A++LV+SD D+S+RM F
Sbjct: 128 FVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFF 187

Query: 190 HTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAF 249
           HT DIMDVT + L+ F+VVFLAALVGM KEEKVKVIEHL+K+M  G++LMLRSA+G RAF
Sbjct: 188 HTVDIMDVT-ESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAF 247

Query: 250 LYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LYP+VE  DL+GFE+L+ +HPTDDV+NS+V+++
Sbjct: 248 LYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of CmaCh12G010280 vs. ExPASy Swiss-Prot
Match: Q9FF79 (Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1)

HSP 1 Score: 308.1 bits (788), Expect = 1.0e-82
Identity = 160/284 (56.34%), Postives = 219/284 (77.11%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           M C     L+V+++ +LY +IS L+SL+PSK+V++LF QLV TC       P+   ID++
Sbjct: 1   MACQNN--LVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL------PTDTNIDVT 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILAS---HQNPLDHLSLFPYYSNYLKLTHL 120
            +   V+ MR++LIKLCG+AEG LE H+STIL S    QNPLDHL +FPYYSNYLKL  L
Sbjct: 61  NMCEEVKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKL 120

Query: 121 EFSILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVA 180
           EF +L+++ S  +P+K+AF+GSG +P +SI++A  +LP T FHNFD+DS AN  A+ LV+
Sbjct: 121 EFDLLSQH-SSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVS 180

Query: 181 SDVDLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLL 240
            D DLS+RM FHTTD+++ T + L +++VVFLAALVGM+KE KVK IEHL K+M  G++L
Sbjct: 181 RDPDLSKRMIFHTTDVLNAT-EGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVL 240

Query: 241 MLRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           MLRSA+  RAFLYP+V++SDL+GF++LT +HPTDDVVNS+V+AR
Sbjct: 241 MLRSAHALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of CmaCh12G010280 vs. ExPASy Swiss-Prot
Match: Q9FKT9 (Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 7.1e-81
Identity = 158/283 (55.83%), Postives = 220/283 (77.74%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           M C  E  L+V+++ +LY++IS L+SL+PSK+V++LF QLV TC       P+   ID++
Sbjct: 1   MAC--ENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL------PTDTNIDVT 60

Query: 61  KL-SSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLE 120
           ++    V+ MRSHLIKLCG+AEG LE H+S IL S + NPL+HL +FPYY+NYLKL  LE
Sbjct: 61  EIHDEKVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLE 120

Query: 121 FSILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVAS 180
           F +L+++ +  +P+KVAFIGSG +P +SI++A  +LP T FHNFD+DS AN  A+ LV+ 
Sbjct: 121 FDLLSQH-TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSR 180

Query: 181 DVDLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLM 240
           D DLS+RM FHTTD+++   + L +++VVFLAALVGM+KE KVK IEHL K+M  G+++M
Sbjct: 181 DSDLSKRMIFHTTDVLN-AKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVM 240

Query: 241 LRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LRSA+G RAFLYP+V++ DL+GFE+LT +HP+DDVVNS+V+AR
Sbjct: 241 LRSAHGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of CmaCh12G010280 vs. ExPASy TrEMBL
Match: A0A6J1HS54 (nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1)

HSP 1 Score: 547.0 bits (1408), Expect = 4.7e-152
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180
           ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of CmaCh12G010280 vs. ExPASy TrEMBL
Match: A0A6J1FHL8 (nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 SV=1)

HSP 1 Score: 536.2 bits (1380), Expect = 8.3e-149
Identity = 277/282 (98.23%), Postives = 279/282 (98.94%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180
           ILTRYIS +LPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAT LVASDV
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDV 180

Query: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLSRRM+FHTTDIMDVTSDML EFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of CmaCh12G010280 vs. ExPASy TrEMBL
Match: A0A1S3BXJ6 (nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1)

HSP 1 Score: 438.0 bits (1125), Expect = 3.1e-119
Identity = 229/282 (81.21%), Postives = 251/282 (89.01%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCC  + LLLVQK+SELYHKISTL+SL+PSKHVNSLFSQLVL    PP    SP  ID+S
Sbjct: 1   MCC--QSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICTPPMASSSPSSIDVS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            L S++QTMRS+LIKLCG+AE LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  SLCSSIQTMRSNLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180
           IL  +I+P  PSKVAF+GSGALPFSSIIMALKYLP TEFHNFD+D SANAKA+MLVA DV
Sbjct: 121 ILNHHITPP-PSKVAFVGSGALPFSSIIMALKYLPKTEFHNFDLDPSANAKASMLVAGDV 180

Query: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           +LSRRM FHTTDIM+V S+ LREFEVVFLAALVGME+EEK KVIEHLRKNM  G+LLMLR
Sbjct: 181 ELSRRMVFHTTDIMEVGSEELREFEVVFLAALVGMEREEKAKVIEHLRKNMRGGALLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEA DLRGFEILT FHPTD+V+NSIVLARL
Sbjct: 241 SAYGARAFLYPVVEACDLRGFEILTVFHPTDEVINSIVLARL 279

[Close]

BLAST of CmaCh12G010280 vs. ExPASy TrEMBL
Match: A0A0A0LJ09 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1)

HSP 1 Score: 427.2 bits (1097), Expect = 5.4e-116
Identity = 227/286 (79.37%), Postives = 249/286 (87.06%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLV-LTCTPPPPPPPSPPLIDI 60
           MCC  + LLLVQK+SELYHKISTL+SL+PSKHVNSLFSQLV L CT      PS   ID+
Sbjct: 1   MCC--QSLLLVQKISELYHKISTLESLKPSKHVNSLFSQLVLLICT------PSSSSIDV 60

Query: 61  SKLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEF 120
           S L ST+QTMRSHLIKLCG+AE LLEHHYSTIL+SHQNPLDHLSLFPYYSNYLKLTHLEF
Sbjct: 61  STLCSTIQTMRSHLIKLCGEAESLLEHHYSTILSSHQNPLDHLSLFPYYSNYLKLTHLEF 120

Query: 121 SILTRYI---SPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLV 180
           SIL  +    +   PSKVAF+GSGALPFSSIIMALKY P TEFHNFD+D SANAKA+MLV
Sbjct: 121 SILNHHAPHSTTTPPSKVAFVGSGALPFSSIIMALKYFPKTEFHNFDLDPSANAKASMLV 180

Query: 181 ASDVDLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSL 240
           A DV+LSRRM FHTTDIMDV S+ LREFEVVFLAALVGME+EEK KVIEHLRKNM+ G+L
Sbjct: 181 AGDVELSRRMVFHTTDIMDVGSEELREFEVVFLAALVGMEREEKGKVIEHLRKNMSGGAL 240

Query: 241 LMLRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           LMLRSAYGARAF+YPVVEA DLRGF+ILT FHPTD+V+NSIVLARL
Sbjct: 241 LMLRSAYGARAFVYPVVEACDLRGFDILTVFHPTDEVINSIVLARL 278

[Close]

BLAST of CmaCh12G010280 vs. ExPASy TrEMBL
Match: A0A6J1DTD8 (probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309 PE=3 SV=1)

HSP 1 Score: 419.9 bits (1078), Expect = 8.7e-114
Identity = 223/282 (79.08%), Postives = 245/282 (86.88%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCC  + +LLVQKVSELYHKISTLQSL+PSK VNSLFSQLVLTC    PPPPSPP ID+S
Sbjct: 1   MCC--QEVLLVQKVSELYHKISTLQSLKPSKDVNSLFSQLVLTCA---PPPPSPPPIDVS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KL S VQ MRSHLIKLCG+AE LLEHHYS+ILASH+NPL++LS+FPYYSNYLKL HLEFS
Sbjct: 61  KLGSAVQKMRSHLIKLCGEAEALLEHHYSSILASHENPLENLSIFPYYSNYLKLAHLEFS 120

Query: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180
           IL R+   A PSKVAF+GSGALPF+SIIMA KYLP TEFHNFDVD SANAKA  LVA D 
Sbjct: 121 ILERH---AQPSKVAFVGSGALPFTSIIMASKYLPATEFHNFDVDPSANAKAAALVAGDR 180

Query: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DL RRM FHT DIM+VT + LR F+VVFLAALVGME+EEKVKVIEHL KNM  G+LLMLR
Sbjct: 181 DLERRMVFHTADIMEVTGE-LRGFQVVFLAALVGMEREEKVKVIEHLGKNMGGGALLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEA DL+GFEILT FHPTD+V+NS+VLARL
Sbjct: 241 SAYGARAFLYPVVEARDLQGFEILTVFHPTDEVINSVVLARL 273

[Close]

BLAST of CmaCh12G010280 vs. NCBI nr
Match: XP_022965879.1 (nicotianamine synthase [Cucurbita maxima])

HSP 1 Score: 547.0 bits (1408), Expect = 9.7e-152
Identity = 282/282 (100.00%), Postives = 282/282 (100.00%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180
           ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV
Sbjct: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180

Query: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of CmaCh12G010280 vs. NCBI nr
Match: XP_022938003.1 (nicotianamine synthase-like [Cucurbita moschata])

HSP 1 Score: 536.2 bits (1380), Expect = 1.7e-148
Identity = 277/282 (98.23%), Postives = 279/282 (98.94%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180
           ILTRYIS +LPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAT LVASDV
Sbjct: 121 ILTRYISSSLPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATALVASDV 180

Query: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLSRRM+FHTTDIMDVTSDML EFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSRRMSFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of CmaCh12G010280 vs. NCBI nr
Match: KAG7021049.1 (Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 529.6 bits (1363), Expect = 1.6e-146
Identity = 274/282 (97.16%), Postives = 277/282 (98.23%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180
           ILTRYIS + PSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAT LVASDV
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180

Query: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLS+RM FHTTDIMDVTS+ML EFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSKRMLFHTTDIMDVTSNMLGEFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of CmaCh12G010280 vs. NCBI nr
Match: KAG6586217.1 (putative nicotianamine synthase 4, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 528.9 bits (1361), Expect = 2.7e-146
Identity = 273/282 (96.81%), Postives = 277/282 (98.23%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQ+LQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQTLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
           KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180
           ILTRYIS + PSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAT LVASDV
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATTLVASDV 180

Query: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLS+RM FHTTDIMDVTSDML EFEVVFLAALVGMEKEEKVKVIEHL+KNMTSGSLLMLR
Sbjct: 181 DLSKRMLFHTTDIMDVTSDMLGEFEVVFLAALVGMEKEEKVKVIEHLQKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of CmaCh12G010280 vs. NCBI nr
Match: XP_023538584.1 (nicotianamine synthase-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 527.7 bits (1358), Expect = 6.1e-146
Identity = 272/282 (96.45%), Postives = 276/282 (97.87%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLID+S
Sbjct: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDVS 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120
            LSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS
Sbjct: 61  NLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQNPLDHLSLFPYYSNYLKLTHLEFS 120

Query: 121 ILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDV 180
           ILTRYIS + PSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKA  LVASDV
Sbjct: 121 ILTRYISSSPPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKAMALVASDV 180

Query: 181 DLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240
           DLS+RM FHTTDIMDVTS+MLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR
Sbjct: 181 DLSKRMLFHTTDIMDVTSNMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLR 240

Query: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 283
           SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL
Sbjct: 241 SAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLARL 282

[Close]

BLAST of CmaCh12G010280 vs. TAIR 10
Match: AT1G56430.1 (nicotianamine synthase 4 )

HSP 1 Score: 317.0 bits (811), Expect = 1.5e-86
Identity = 162/273 (59.34%), Postives = 215/273 (78.75%), Query Frame = 0


Query: 10  LVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTM 69
           LV K+ +LY KIS L++L+P + V++LF QLV TC PP P       ID++K+S  +Q M
Sbjct: 9   LVNKICDLYEKISKLETLKPCEDVDTLFKQLVSTCIPPNPN------IDVTKMSENIQEM 68

Query: 70  RSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILTRYISP 129
           RS+LIK+CG+AEG LEHH+S+IL S + NPL HL+LFPYY+NYLKL+ LEF +L + ++ 
Sbjct: 69  RSNLIKICGEAEGYLEHHFSSILTSFEDNPLHHLNLFPYYNNYLKLSKLEFDLLEQNLNG 128

Query: 130 ALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDVDLSRRMTF 189
            +P  VAFIGSG LP +S+++A  +L  + FHNFD+D SAN  A  LV+SD DLS+RM F
Sbjct: 129 FVPRTVAFIGSGPLPLTSVVLASSHLKDSIFHNFDIDPSANMVAARLVSSDPDLSQRMFF 188

Query: 190 HTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAF 249
           HT DIMDVT + L+ F+VVFLAALVGM+K+EKVKV+EHL K+M+ G+LLMLRSA+G RAF
Sbjct: 189 HTVDIMDVT-ESLKGFDVVFLAALVGMDKKEKVKVVEHLEKHMSPGALLMLRSAHGPRAF 248

Query: 250 LYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LYP+VE  DL GFE+L+ +HPTD+V+NSIV++R
Sbjct: 249 LYPIVEPCDLEGFEVLSVYHPTDEVINSIVISR 274

[Close]

BLAST of CmaCh12G010280 vs. TAIR 10
Match: AT1G09240.1 (nicotianamine synthase 3 )

HSP 1 Score: 313.9 bits (803), Expect = 1.3e-85
Identity = 163/273 (59.71%), Postives = 215/273 (78.75%), Query Frame = 0


Query: 10  LVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDISKLSSTVQTM 69
           LVQ + +LY KIS L+SL+PS+ VN LF QLV TC PP P       ID++K+   VQ +
Sbjct: 8   LVQTICDLYEKISKLESLKPSEDVNILFKQLVSTCIPPNPN------IDVTKMCDRVQEI 67

Query: 70  RSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLEFSILTRYISP 129
           R +LIK+CG AEG LE+H+S+IL S+Q NPL HL++FPYY+NYLKL  LEF +L + ++ 
Sbjct: 68  RLNLIKICGLAEGHLENHFSSILTSYQDNPLHHLNIFPYYNNYLKLGKLEFDLLEQNLNG 127

Query: 130 ALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVASDVDLSRRMTF 189
            +P  VAFIGSG LP +SI++A  +L  T FHNFD+D SAN+ A++LV+SD D+S+RM F
Sbjct: 128 FVPKSVAFIGSGPLPLTSIVLASFHLKDTIFHNFDIDPSANSLASLLVSSDPDISQRMFF 187

Query: 190 HTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLMLRSAYGARAF 249
           HT DIMDVT + L+ F+VVFLAALVGM KEEKVKVIEHL+K+M  G++LMLRSA+G RAF
Sbjct: 188 HTVDIMDVT-ESLKSFDVVFLAALVGMNKEEKVKVIEHLQKHMAPGAVLMLRSAHGPRAF 247

Query: 250 LYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LYP+VE  DL+GFE+L+ +HPTDDV+NS+V+++
Sbjct: 248 LYPIVEPCDLQGFEVLSIYHPTDDVINSVVISK 273

[Close]

BLAST of CmaCh12G010280 vs. TAIR 10
Match: AT5G04950.1 (nicotianamine synthase 1 )

HSP 1 Score: 308.1 bits (788), Expect = 7.1e-84
Identity = 160/284 (56.34%), Postives = 219/284 (77.11%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           M C     L+V+++ +LY +IS L+SL+PSK+V++LF QLV TC       P+   ID++
Sbjct: 1   MACQNN--LVVKQIIDLYDQISKLKSLKPSKNVDTLFGQLVSTCL------PTDTNIDVT 60

Query: 61  KLSSTVQTMRSHLIKLCGQAEGLLEHHYSTILAS---HQNPLDHLSLFPYYSNYLKLTHL 120
            +   V+ MR++LIKLCG+AEG LE H+STIL S    QNPLDHL +FPYYSNYLKL  L
Sbjct: 61  NMCEEVKDMRANLIKLCGEAEGYLEQHFSTILGSLQEDQNPLDHLHIFPYYSNYLKLGKL 120

Query: 121 EFSILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVA 180
           EF +L+++ S  +P+K+AF+GSG +P +SI++A  +LP T FHNFD+DS AN  A+ LV+
Sbjct: 121 EFDLLSQH-SSHVPTKIAFVGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVS 180

Query: 181 SDVDLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLL 240
            D DLS+RM FHTTD+++ T + L +++VVFLAALVGM+KE KVK IEHL K+M  G++L
Sbjct: 181 RDPDLSKRMIFHTTDVLNAT-EGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVL 240

Query: 241 MLRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           MLRSA+  RAFLYP+V++SDL+GF++LT +HPTDDVVNS+V+AR
Sbjct: 241 MLRSAHALRAFLYPIVDSSDLKGFQLLTIYHPTDDVVNSVVIAR 274

[Close]

BLAST of CmaCh12G010280 vs. TAIR 10
Match: AT5G56080.1 (nicotianamine synthase 2 )

HSP 1 Score: 302.0 bits (772), Expect = 5.1e-82
Identity = 158/283 (55.83%), Postives = 220/283 (77.74%), Query Frame = 0


Query: 1   MCCDGEGLLLVQKVSELYHKISTLQSLQPSKHVNSLFSQLVLTCTPPPPPPPSPPLIDIS 60
           M C  E  L+V+++ +LY++IS L+SL+PSK+V++LF QLV TC       P+   ID++
Sbjct: 1   MAC--ENNLVVKQIMDLYNQISNLESLKPSKNVDTLFRQLVSTCL------PTDTNIDVT 60

Query: 61  KL-SSTVQTMRSHLIKLCGQAEGLLEHHYSTILASHQ-NPLDHLSLFPYYSNYLKLTHLE 120
           ++    V+ MRSHLIKLCG+AEG LE H+S IL S + NPL+HL +FPYY+NYLKL  LE
Sbjct: 61  EIHDEKVKDMRSHLIKLCGEAEGYLEQHFSAILGSFEDNPLNHLHIFPYYNNYLKLGKLE 120

Query: 121 FSILTRYISPALPSKVAFIGSGALPFSSIIMALKYLPTTEFHNFDVDSSANAKATMLVAS 180
           F +L+++ +  +P+KVAFIGSG +P +SI++A  +LP T FHNFD+DS AN  A+ LV+ 
Sbjct: 121 FDLLSQH-TTHVPTKVAFIGSGPMPLTSIVLAKFHLPNTTFHNFDIDSHANTLASNLVSR 180

Query: 181 DVDLSRRMTFHTTDIMDVTSDMLREFEVVFLAALVGMEKEEKVKVIEHLRKNMTSGSLLM 240
           D DLS+RM FHTTD+++   + L +++VVFLAALVGM+KE KVK IEHL K+M  G+++M
Sbjct: 181 DSDLSKRMIFHTTDVLN-AKEGLDQYDVVFLAALVGMDKESKVKAIEHLEKHMAPGAVVM 240

Query: 241 LRSAYGARAFLYPVVEASDLRGFEILTTFHPTDDVVNSIVLAR 282
           LRSA+G RAFLYP+V++ DL+GFE+LT +HP+DDVVNS+V+AR
Sbjct: 241 LRSAHGLRAFLYPIVDSCDLKGFEVLTIYHPSDDVVNSVVIAR 273

[Close]

BLAST of CmaCh12G010280 vs. TAIR 10
Match: AT4G26483.1 (nicotianamine synthases )

HSP 1 Score: 58.2 bits (139), Expect = 1.3e-08
Identity = 29/53 (54.72%), Postives = 39/53 (73.58%), Query Frame = 0


Query: 231 MTSGSLLMLRSAYGARAFLYPVVEASDLRGFEILTTFHP--TDDVVNSIVLAR 282
           M  G+LLMLRSA G R+FLY  V+  DL+GFE+L  +HP  +D  VNS+++AR
Sbjct: 1   MDPGALLMLRSARGLRSFLYVDVDPCDLKGFEVLEIYHPSMSDGFVNSVMVAR 53

The following BLAST results are available for this feature:

BLAST of CmaCh12G010280 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9XGI7	3.9e-87	59.56	Nicotianamine synthase OS=Solanum lycopersicum OX=4081 GN=CHLN PE=2 SV=1	[more]
Q9C7X5	2.1e-85	59.34	Probable nicotianamine synthase 4 OS=Arabidopsis thaliana OX=3702 GN=NAS4 PE=2 S...	[more]
O80483	1.8e-84	59.71	Nicotianamine synthase 3 OS=Arabidopsis thaliana OX=3702 GN=NAS3 PE=2 SV=1	[more]
Q9FF79	1.0e-82	56.34	Nicotianamine synthase 1 OS=Arabidopsis thaliana OX=3702 GN=NAS1 PE=2 SV=1	[more]
Q9FKT9	7.1e-81	55.83	Nicotianamine synthase 2 OS=Arabidopsis thaliana OX=3702 GN=NAS2 PE=2 SV=1	[more]

BLAST of CmaCh12G010280 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HS54	4.7e-152	100.00	nicotianamine synthase OS=Cucurbita maxima OX=3661 GN=LOC111465632 PE=3 SV=1	[more]
A0A6J1FHL8	8.3e-149	98.23	nicotianamine synthase-like OS=Cucurbita moschata OX=3662 GN=LOC111444226 PE=3 S...	[more]
A0A1S3BXJ6	3.1e-119	81.21	nicotianamine synthase 1-like OS=Cucumis melo OX=3656 GN=LOC103494189 PE=3 SV=1	[more]
A0A0A0LJ09	5.4e-116	79.37	Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G034520 PE=3 SV=1	[more]
A0A6J1DTD8	8.7e-114	79.08	probable nicotianamine synthase 4 OS=Momordica charantia OX=3673 GN=LOC111023309...	[more]

BLAST of CmaCh12G010280 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022965879.1	9.7e-152	100.00	nicotianamine synthase [Cucurbita maxima]	[more]
XP_022938003.1	1.7e-148	98.23	nicotianamine synthase-like [Cucurbita moschata]	[more]
KAG7021049.1	1.6e-146	97.16	Nicotianamine synthase, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]
KAG6586217.1	2.7e-146	96.81	putative nicotianamine synthase 4, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_023538584.1	6.1e-146	96.45	nicotianamine synthase-like [Cucurbita pepo subsp. pepo]	[more]

BLAST of CmaCh12G010280 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G56430.1	1.5e-86	59.34	nicotianamine synthase 4	[more]
AT1G09240.1	1.3e-85	59.71	nicotianamine synthase 3	[more]
AT5G04950.1	7.1e-84	56.34	nicotianamine synthase 1	[more]
AT5G56080.1	5.1e-82	55.83	nicotianamine synthase 2	[more]
AT4G26483.1	1.3e-08	54.72	nicotianamine synthases	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	GENE3D	3.40.50.150	Vaccinia Virus protein VP39	coord: 56..282 e-value: 1.0E-58 score: 200.5
None	No IPR available	PANTHER	PTHR32266:SF17	SYNTHASE, PUTATIVE-RELATED	coord: 3..281
IPR004298	Nicotianamine synthase	PFAM	PF03059	NAS	coord: 9..281 e-value: 7.5E-112 score: 373.2
IPR004298	Nicotianamine synthase	PANTHER	PTHR32266	NICOTIANAMINE SYNTHASE 3	coord: 3..281
IPR004298	Nicotianamine synthase	PROSITE	PS51142	NAS	coord: 2..282 score: 51.625706

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G010280.1	CmaCh12G010280.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030418	nicotianamine biosynthetic process
molecular_function	GO:0030410	nicotianamine synthase activity