CmaCh12G010150 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G010150
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	PMEI domain-containing protein
Location	Cma_Chr12: 8073663 .. 8074487 (-)
RNA-Seq Expression	CmaCh12G010150
Synteny	CmaCh12G010150

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGTTACTCTAAAATCTCACCTCCTTCATCCCTGCTATTCTTTTGGCAGCTTTATGCAATCCTCCATGAAAATCTGGTCACCATTTGCTCTACAACCTCGTACCCGTTGCACTGCCAGAAAATCCTCACCAACGATTCCCATACTATCTCGGTCGACCTCCCCGAACTCTCGCTTATATCCATTAATCTAACAATGAAGCAGGCCGATAGAAACCTGGATTCCTTCTCCAAACTAAGTAAGAATACGAGTGATCCCAAGCAGAAGAAGAGTTTTGACCGTTGTGTAGAGTATTACCACAAAATCAAAACAATGCTCAATCTTTTATTGTTCTTTAAGCACCAACCATGCTGAATTTGCAAAGCTAGAGGTCCCTAAGGATCCTTGCAAACTTTCACAAGTAGAGCTACCTTAACAGATGATTCCAACCAGAGCTGGTTAGAAAGATGAATAACTCTTAGATTAAACTTGGAATCTATGTTTGTCACTCTTAGATACTCTTAGATACGCTCACTCCAGAATTAGCTTGATCAAGATTAATCAAATGAACCAGTTTAACATCAAACCTAAAACAAGACACGGCACTACGAAAGACAAAAAGTCTTCAAATCACTCTCCAATGTCGAGTGATATTTATAGTCATTCCATTCTTTTCTTGATTTGGTACTTAAATATTTTTTGTCTTACTTCTTGTAATGGCTCCTTCGGGTACATTCGGGAACATACAAAAGAGATAAAGTTGTAAAAATTTGGGGATATATGGAAATGAAAAGCCAATCAAGGAAGTTTAGAAAAGGGTATTATAACATTTAAGGAAGGAATTGA

mRNA sequence

ATGGGTTACTCTAAAATCTCACCTCCTTCATCCCTGCTATTCTTTTGGCAGCTTTATGCAATCCTCCATGAAAATCTGGTCACCATTTGCTCTACAACCTCGTACCCGTTGCACTGCCAGAAAATCCTCACCAACGATTCCCATACTATCTCGGTCGACCTCCCCGAACTCTCGCTTATATCCATTAATCTAACAATGAAGCAGGCCGATAGAAACCTGGATTCCTTCTCCAAACTAAGTAAGAATACGAGTGATCCCAAGCAGAAGAAGAGTTTTGACCGAAGGAATTGA

Coding sequence (CDS)

ATGGGTTACTCTAAAATCTCACCTCCTTCATCCCTGCTATTCTTTTGGCAGCTTTATGCAATCCTCCATGAAAATCTGGTCACCATTTGCTCTACAACCTCGTACCCGTTGCACTGCCAGAAAATCCTCACCAACGATTCCCATACTATCTCGGTCGACCTCCCCGAACTCTCGCTTATATCCATTAATCTAACAATGAAGCAGGCCGATAGAAACCTGGATTCCTTCTCCAAACTAAGTAAGAATACGAGTGATCCCAAGCAGAAGAAGAGTTTTGACCGAAGGAATTGA

Protein sequence

MGYSKISPPSSLLFFWQLYAILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLISINLTMKQADRNLDSFSKLSKNTSDPKQKKSFDRRN

Homology

[Close]

BLAST of CmaCh12G010150 vs. ExPASy TrEMBL
Match: A0A5A7U3I1 (Pectinesterase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold385G001900 PE=4 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 4.4e-17
Identity = 51/75 (68.00%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 19  YAILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLISINLTMKQADRNLDSFSK 78
           + I HENLVTICS TS P  C+KIL NDS T+S DLP+LSLI  NL  KQAD+NLD+F K
Sbjct: 27  HGIPHENLVTICSKTSNPSLCEKILNNDSRTVSADLPKLSLICSNLAKKQADQNLDTFYK 86

Query: 79  LSKNTSDPKQKKSFD 94
           LSKN SDP+ KKSF+
Sbjct: 87  LSKNESDPEVKKSFE 101

[Close]

BLAST of CmaCh12G010150 vs. ExPASy TrEMBL
Match: A0A0A0LK13 (PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G034700 PE=4 SV=1)

HSP 1 Score: 96.7 bits (239), Expect = 5.7e-17
Identity = 50/75 (66.67%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 19  YAILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLISINLTMKQADRNLDSFSK 78
           + I HENLVTICS TS P  C+KIL NDS T+S +LP+LSLI  NL  KQAD+NLD+F K
Sbjct: 27  HGIPHENLVTICSKTSNPSLCEKILNNDSRTVSANLPKLSLICSNLAKKQADQNLDTFYK 86

Query: 79  LSKNTSDPKQKKSFD 94
           LSKN SDP++KKSF+
Sbjct: 87  LSKNESDPEEKKSFE 101

[Close]

BLAST of CmaCh12G010150 vs. ExPASy TrEMBL
Match: A0A061F340 (PMEI domain-containing protein OS=Theobroma cacao OX=3641 GN=TCM_026801 PE=4 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 9.8e-09
Identity = 42/93 (45.16%), Postives = 53/93 (56.99%), Query Frame = 0


Query: 1  MGYSKISPPSSLLFFWQLYAILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLI 60
          + +S I P SS         + +E LV ICS T  P+ C  IL  D  T S DLPELSL+
Sbjct: 15 VAFSVIHPSSS---------VPNETLVAICSKTYNPILCASILRGDPRTSSADLPELSLV 74

Query: 61 SINLTMKQADRNLDSFSKLSKNTSDPKQKKSFD 94
          SINLT++QA  N   F +   NT+DP  KK F+
Sbjct: 75 SINLTIEQAANNSQVFVEFHDNTTDPALKKGFN 98

[Close]

BLAST of CmaCh12G010150 vs. ExPASy TrEMBL
Match: A0A6A1UMD5 (Pectinesterase inhibitor OS=Morella rubra OX=262757 GN=CJ030_MR0G004165 PE=4 SV=1)

HSP 1 Score: 67.8 bits (164), Expect = 2.8e-08
Identity = 38/72 (52.78%), Postives = 48/72 (66.67%), Query Frame = 0


Query: 21 ILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLISINLTMKQADRNLDSFSKLS 80
          + H+ L TICS T     C++ L +DS T S DLP+LSLISI LTMKQAD N  +FS L 
Sbjct: 26 VSHDILTTICSKTQNHDACERSLESDSRTRSADLPKLSLISIELTMKQADHNHRTFSDLR 85

Query: 81 KNTSDPKQKKSF 93
           N +DP+ + SF
Sbjct: 86 DNATDPRLEDSF 97

[Close]

BLAST of CmaCh12G010150 vs. ExPASy TrEMBL
Match: A0A7N2M2Y0 (PMEI domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1)

HSP 1 Score: 63.9 bits (154), Expect = 4.1e-07
Identity = 36/74 (48.65%), Postives = 45/74 (60.81%), Query Frame = 0


Query: 19 YAILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLISINLTMKQADRNLDSFSK 78
          Y +  + L TICS T     C+ ILT+D  T S DLP LSLIS+ LTMKQA  N  SF++
Sbjct: 25 YGVNPDVLATICSQTQNQEICESILTSDPRTSSADLPTLSLISLELTMKQASDNNQSFTQ 84

Query: 79 LSKNTSDPKQKKSF 93
             NT++P  K  F
Sbjct: 85 FRDNTTNPSLKNDF 98

[Close]

BLAST of CmaCh12G010150 vs. NCBI nr
Match: KAA0048111.1 (pectinesterase inhibitor [Cucumis melo var. makuwa])

HSP 1 Score: 97.1 bits (240), Expect = 9.1e-17
Identity = 51/75 (68.00%), Postives = 59/75 (78.67%), Query Frame = 0


Query: 19  YAILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLISINLTMKQADRNLDSFSK 78
           + I HENLVTICS TS P  C+KIL NDS T+S DLP+LSLI  NL  KQAD+NLD+F K
Sbjct: 27  HGIPHENLVTICSKTSNPSLCEKILNNDSRTVSADLPKLSLICSNLAKKQADQNLDTFYK 86

Query: 79  LSKNTSDPKQKKSFD 94
           LSKN SDP+ KKSF+
Sbjct: 87  LSKNESDPEVKKSFE 101

[Close]

BLAST of CmaCh12G010150 vs. NCBI nr
Match: KAE8651630.1 (hypothetical protein Csa_021165 [Cucumis sativus])

HSP 1 Score: 96.7 bits (239), Expect = 1.2e-16
Identity = 50/75 (66.67%), Postives = 60/75 (80.00%), Query Frame = 0


Query: 19  YAILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLISINLTMKQADRNLDSFSK 78
           + I HENLVTICS TS P  C+KIL NDS T+S +LP+LSLI  NL  KQAD+NLD+F K
Sbjct: 516 HGIPHENLVTICSKTSNPSLCEKILNNDSRTVSANLPKLSLICSNLAKKQADQNLDTFYK 575

Query: 79  LSKNTSDPKQKKSFD 94
           LSKN SDP++KKSF+
Sbjct: 576 LSKNESDPEEKKSFE 590

[Close]

BLAST of CmaCh12G010150 vs. NCBI nr
Match: KAG6599172.1 (hypothetical protein SDJN03_08950, partial [Cucurbita argyrosperma subsp. sororia] >KAG7030110.1 hypothetical protein SDJN02_08457, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 93.2 bits (230), Expect = 1.3e-15
Identity = 51/74 (68.92%), Postives = 56/74 (75.68%), Query Frame = 0


Query: 19 YAILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLISINLTMKQADRNLDSFSK 78
          + ILH NL TIC  T  P  CQ+IL N   TIS DLP+LSLISINLT  QAD+NLDSFSK
Sbjct: 23 HGILHGNLATICYKTPDPFSCQRILGNVPGTISADLPKLSLISINLTKNQADQNLDSFSK 82

Query: 79 LSKNTSDPKQKKSF 93
          LSKN SDP+ KKSF
Sbjct: 83 LSKNESDPELKKSF 96

[Close]

BLAST of CmaCh12G010150 vs. NCBI nr
Match: XP_007031213.2 (PREDICTED: pectinesterase inhibitor [Theobroma cacao])

HSP 1 Score: 70.5 bits (171), Expect = 9.1e-09
Identity = 42/93 (45.16%), Postives = 54/93 (58.06%), Query Frame = 0


Query: 1  MGYSKISPPSSLLFFWQLYAILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLI 60
          + +S I P SS         + +E LV ICS T  P+ C+ IL  D  T S DLPELSL+
Sbjct: 15 VAFSVIHPSSS---------VPNETLVAICSKTYNPILCESILRGDPRTSSADLPELSLV 74

Query: 61 SINLTMKQADRNLDSFSKLSKNTSDPKQKKSFD 94
          SINLT++QA  N   F +   NT+DP  KK F+
Sbjct: 75 SINLTIEQAANNSQVFVEFHDNTTDPALKKGFN 98

[Close]

BLAST of CmaCh12G010150 vs. NCBI nr
Match: EOY11715.1 (Uncharacterized protein TCM_026801 [Theobroma cacao])

HSP 1 Score: 69.3 bits (168), Expect = 2.0e-08
Identity = 42/93 (45.16%), Postives = 53/93 (56.99%), Query Frame = 0


Query: 1  MGYSKISPPSSLLFFWQLYAILHENLVTICSTTSYPLHCQKILTNDSHTISVDLPELSLI 60
          + +S I P SS         + +E LV ICS T  P+ C  IL  D  T S DLPELSL+
Sbjct: 15 VAFSVIHPSSS---------VPNETLVAICSKTYNPILCASILRGDPRTSSADLPELSLV 74

Query: 61 SINLTMKQADRNLDSFSKLSKNTSDPKQKKSFD 94
          SINLT++QA  N   F +   NT+DP  KK F+
Sbjct: 75 SINLTIEQAANNSQVFVEFHDNTTDPALKKGFN 98

The following BLAST results are available for this feature:

BLAST of CmaCh12G010150 vs.


Match Name	E-value	Identity	Description

BLAST of CmaCh12G010150 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A5A7U3I1	4.4e-17	68.00	Pectinesterase inhibitor OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffol...	[more]
A0A0A0LK13	5.7e-17	66.67	PMEI domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G034700 PE=4 S...	[more]
A0A061F340	9.8e-09	45.16	PMEI domain-containing protein OS=Theobroma cacao OX=3641 GN=TCM_026801 PE=4 SV=...	[more]
A0A6A1UMD5	2.8e-08	52.78	Pectinesterase inhibitor OS=Morella rubra OX=262757 GN=CJ030_MR0G004165 PE=4 SV=...	[more]
A0A7N2M2Y0	4.1e-07	48.65	PMEI domain-containing protein OS=Quercus lobata OX=97700 PE=4 SV=1	[more]

BLAST of CmaCh12G010150 vs. NCBI nr


Match Name	E-value	Identity	Description
KAA0048111.1	9.1e-17	68.00	pectinesterase inhibitor [Cucumis melo var. makuwa]	[more]
KAE8651630.1	1.2e-16	66.67	hypothetical protein Csa_021165 [Cucumis sativus]	[more]
KAG6599172.1	1.3e-15	68.92	hypothetical protein SDJN03_08950, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_007031213.2	9.1e-09	45.16	PREDICTED: pectinesterase inhibitor [Theobroma cacao]	[more]
EOY11715.1	2.0e-08	45.16	Uncharacterized protein TCM_026801 [Theobroma cacao]	[more]

BLAST of CmaCh12G010150 vs. NCBI nr


Match Name	E-value	Identity	Description

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 27..87 e-value: 1.2E-6 score: 28.9
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 26..93 e-value: 5.7E-12 score: 43.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 24..94 e-value: 1.7E-11 score: 46.3
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 28..93
None	No IPR available	MOBIDB_LITE	mobidb-lite	disorder_prediction	coord: 76..96

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G010150.1	CmaCh12G010150.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity