CmaCh12G010120 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G010120
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	(R)-mandelonitrile lyase 1-like
Location	Cma_Chr12: 8062615 .. 8065887 (-)
RNA-Seq Expression	CmaCh12G010120
Synteny	CmaCh12G010120

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

AGCTTTTGAAAACCATAGAGCATTCTATGGCTACCATTCTTCCACTAATTCTTATCTCTATCTTCTTCTTACGAGTCCTCTCCTCCCATACTATCCCTAATCAAGGTAAGAATCCTCTTCGGCACTACCTGCAACAGTTCACCGCTATCAAATATGGTTTTCTTCAAGTTTTTTCTTTCAAATTTTTCCTTGAGATTTTTAGTTCACCCTTGTAAAGGATGTTGGGATATCATATTACATGTTTGTTAATTTTGATTGTTCTAAGCTTTTGTTTTGAAGATGTGAGCTACATGAAGTTAGTTCACGACGCGACCGACATACCCGAAAACGAAGAATATGATTACATAATCATAGGAGGAGGAACAGCTGGTTGCCCATTAGCTGCAACCTTGTCATCGAAATTCTCCGTCCTCCTTCTTGAAAGAGGTAGCGATCCCAACAAGTATCCCTCCGTGTTAAACGAGCAAGGTTTATTGAACGTGTTTGTCGAAGAAGATGATGGTGAAAACCCGTTCCAGCGCTTTGTCTCTGAGAATGGCGTGGAGATCATTAGAGGAAGGGTTCTTGGTGGTGGTAGCATGGTGAATGCTGGGTTCTACTCGAGGGGCCATCGGGAGTTCTTTGAAAGTGCAGGCGTTGATTGGGACATGGAGTTGGTGGAGAAGGGCTATGAATGGGTTGAGGAGACGGTGGTGTCTCGCCCAAGGTTGAGTGCTTGGCAAGGTGCTTTTAGAAGGGCTTTGTTGGAAGGTGGTGTTGGTCCTGATAATGGGTTTGATTTGAGGCATCTTGTGGGCACTAAAACTGGTGGTTCTATCTTTGATGAGGATGGAAATAGACATGGAGCTGTGGAGCTTCTTAATGGGGTTGAACCCGGAAATCTTAAAGTTGCTGTTCGTGCTACTGTTCAGAAAATCATTTTTTCGGGTGAGTAGAAATGTTTTATTAACTTGTTCGAAGGAAATTATAGTTAAGTAAAAAGTTTCCATTTAAACAAGGAATGGAGAACATGATTCTGTGTAACAGCTACGGGAGAGAGGAACGAAATATTTCTCATAAGGGTGTGGAAACCTCTCCGTAACAGACACGTTTTAAAACTGTTAGGCTGATAGCGTACGTAATGGGTCAAAATAGATAATATCTCATAGTGGTGGAATTGGACTATTATAGTGCACCTCTAGCAGATATTGTGTTACTTGAGCTTTCCCTTTTAGGTTTCACCTCGAGGTTTTAAAATGCCTAATAGACGTGTTTTAATACTGTGAGGCTGACAGTGATACGTAAAGGGTCAAAGCGGACAATATCTACTAGTGGTGAGATTGGACTATTATAGCCTACCGGTAGTAGATATTATTCTCTTTAAGCTTTCCCTTCCGGGCTTTCCCTCAAGGTTTTAAAACACGTATAATAGAGTTTTCCACACCCTTATAAAGAATAATTCTACTAGTGGCGGGATTGGCCTGATATAGTCCACCGCTTTCTCTTTCGAGTTTTCGCTCAAGGTTTCTACACCCTTATAAAGAATAATTCTGCTAGTGGCGAGATTGGGCTGTTACTTAGAGGGTTACACAATTCCCGTATTTATAGCTTATAGCTCATATCTCAACACACTTTCTTTCAAGGTTTTAAAACGGTATGAGAGTTTTCCACACCTTTGTAAATAATGATTCTGCTAGTGGTGGGATTGAGCTGTTATAACTCACTCTAACATATATTGTCCTCGCTTTCCCTTTAGGGCTTCCCATCAAGGTTTTAAAATGTGTATGCTAGGAAGAGGTTTTCACACCCTCGTAAACAATGCTTCGTTCTCCTCCCCAACTGTGGGGTCTCACAATCCACTTCATTCAGGGCATTCGTTTCCCTCTCGAATCGGTGTGGAAACCTCTCCCTAGAATACACATCTTAAAACCTTGAGAGGAAACTCGAGATGAAAAACTCAAAGAGGACAATATCTACTAGCTATCAACTTAGACTGTAACACTCCCTTTTAGCCAAACAGCGCACAAGTCCGGGACATGAAAACCATTCCTGTCTCCACTCCACGGGAAAGCTTTCCTCATCTCCGTCCCATGGACATTTCAATTGATCCGTCCTCATCACCCGATTCATAAATCTATACACTAACTTTTCATTATCATGAACTCATCACAGATCTATCAGCAATAGGAGTTTCATACTCCGATTCAGAAGGAAAGACACACACCGCATTCATCCGCAAGAAAGGGGAGATCATATTGAGCGCCGGAGCCATTGGAAGCCCTCAGCTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCCTCCCTCAAACTCCCCGTCGTCCTCCACCAACCCCACGTCGGCGAATTCATGTCCGACAACCCCCGTTTCAGCGCCGCCGTCGTGCTTCCATTCCCACTGGTAGCTTCATCGGCACAAGTCGTCGGAATATTAGACGGCAATATCCATTTGCAAACATTTGCAAGCCCTGCACCATTTTTTATTTTACCAACTTTCGGCCTTCTTCCTCCTCAATCCACTCTGATCAACCCGAGCTTAGCCCTTCTTATCGGAAAATTCTCGGACGTGCTTTCAAAAGGCTCACTTCACCTGAATTCCTCGACAGACGTTAAGAACAACCCAATTGTTCGATTCAATTACTATACGAATCCTAACGATCTTGCACAATGCGTTAGAGGGTTACGTAAAGTGGGGGATTTTCTTAAAACCCGAACTATGGAAGAGATTAAGACGCAAGATTTGGAGGGTAATAAAGGGTTTCAGTTTCTAGGGCCTCCATTGCCGGAAAACTTGTCGGATAATAGCTCCGTCCAAGAGTATTGTAAGGAAACGGTGACCACTTATTGGCATTACCACGGCGGATGTTTGGTCGGGAAAGTTGTCGATCGTAATTACAGAGTCATCGGAATCAAAAATTTGCGTGTCGTAGATGGCTCGACTTTCTCTGTCTCGCCGGGGACTAATCCGATGGCCACCCTCATGATGCTTGGCCGGTGAGTTATTAAATTTATAATATTACATATAATTTTGTTTTTCCTATTTTTAATAAAATAAATTATTCAATTCTCATTTTTTTTTCAATTTTATTTCTTCAGGTATGTTGGCCTTAAGATGCTGCAAGAAAGATCAAGTTAAAGAGCATTCATGTTTTTAATAATTTCGTGATGATAAATACAATATCGGTTTTAAGTAAATTTCCCACTATTTTGTAATCCTTTAACGCATTGGGCAAGATCGTTAAGAATCGAATAATAATTGAATCGAACAA

mRNA sequence

AGCTTTTGAAAACCATAGAGCATTCTATGGCTACCATTCTTCCACTAATTCTTATCTCTATCTTCTTCTTACGAGTCCTCTCCTCCCATACTATCCCTAATCAAGATGTGAGCTACATGAAGTTAGTTCACGACGCGACCGACATACCCGAAAACGAAGAATATGATTACATAATCATAGGAGGAGGAACAGCTGGTTGCCCATTAGCTGCAACCTTGTCATCGAAATTCTCCGTCCTCCTTCTTGAAAGAGGTAGCGATCCCAACAAGTATCCCTCCGTGTTAAACGAGCAAGGTTTATTGAACGTGTTTGTCGAAGAAGATGATGGTGAAAACCCGTTCCAGCGCTTTGTCTCTGAGAATGGCGTGGAGATCATTAGAGGAAGGGTTCTTGGTGGTGGTAGCATGGTGAATGCTGGGTTCTACTCGAGGGGCCATCGGGAGTTCTTTGAAAGTGCAGGCGTTGATTGGGACATGGAGTTGGTGGAGAAGGGCTATGAATGGGTTGAGGAGACGGTGGTGTCTCGCCCAAGGTTGAGTGCTTGGCAAGGTGCTTTTAGAAGGGCTTTGTTGGAAGGTGGTGTTGGTCCTGATAATGGGTTTGATTTGAGGCATCTTGTGGGCACTAAAACTGGTGGTTCTATCTTTGATGAGGATGGAAATAGACATGGAGCTGTGGAGCTTCTTAATGGGGTTGAACCCGGAAATCTTAAAGTTGCTGTTCGTGCTACTGTTCAGAAAATCATTTTTTCGGATCTATCAGCAATAGGAGTTTCATACTCCGATTCAGAAGGAAAGACACACACCGCATTCATCCGCAAGAAAGGGGAGATCATATTGAGCGCCGGAGCCATTGGAAGCCCTCAGCTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCCTCCCTCAAACTCCCCGTCGTCCTCCACCAACCCCACGTCGGCGAATTCATGTCCGACAACCCCCGTTTCAGCGCCGCCGTCGTGCTTCCATTCCCACTGGTAGCTTCATCGGCACAAGTCGTCGGAATATTAGACGGCAATATCCATTTGCAAACATTTGCAAGCCCTGCACCATTTTTTATTTTACCAACTTTCGGCCTTCTTCCTCCTCAATCCACTCTGATCAACCCGAGCTTAGCCCTTCTTATCGGAAAATTCTCGGACGTGCTTTCAAAAGGCTCACTTCACCTGAATTCCTCGACAGACGTTAAGAACAACCCAATTGTTCGATTCAATTACTATACGAATCCTAACGATCTTGCACAATGCGTTAGAGGGTTACGTAAAGTGGGGGATTTTCTTAAAACCCGAACTATGGAAGAGATTAAGACGCAAGATTTGGAGGGTAATAAAGGGTTTCAGTTTCTAGGGCCTCCATTGCCGGAAAACTTGTCGGATAATAGCTCCGTCCAAGAGTATTGTAAGGAAACGGTGACCACTTATTGGCATTACCACGGCGGATGTTTGGTCGGGAAAGTTGTCGATCGTAATTACAGAGTCATCGGAATCAAAAATTTGCGTGTCGTAGATGGCTCGACTTTCTCTGTCTCGCCGGGGACTAATCCGATGGCCACCCTCATGATGCTTGGCCGGTATGTTGGCCTTAAGATGCTGCAAGAAAGATCAAGTTAAAGAGCATTCATGTTTTTAATAATTTCGTGATGATAAATACAATATCGGTTTTAAGTAAATTTCCCACTATTTTGTAATCCTTTAACGCATTGGGCAAGATCGTTAAGAATCGAATAATAATTGAATCGAACAA

Coding sequence (CDS)

ATGGCTACCATTCTTCCACTAATTCTTATCTCTATCTTCTTCTTACGAGTCCTCTCCTCCCATACTATCCCTAATCAAGATGTGAGCTACATGAAGTTAGTTCACGACGCGACCGACATACCCGAAAACGAAGAATATGATTACATAATCATAGGAGGAGGAACAGCTGGTTGCCCATTAGCTGCAACCTTGTCATCGAAATTCTCCGTCCTCCTTCTTGAAAGAGGTAGCGATCCCAACAAGTATCCCTCCGTGTTAAACGAGCAAGGTTTATTGAACGTGTTTGTCGAAGAAGATGATGGTGAAAACCCGTTCCAGCGCTTTGTCTCTGAGAATGGCGTGGAGATCATTAGAGGAAGGGTTCTTGGTGGTGGTAGCATGGTGAATGCTGGGTTCTACTCGAGGGGCCATCGGGAGTTCTTTGAAAGTGCAGGCGTTGATTGGGACATGGAGTTGGTGGAGAAGGGCTATGAATGGGTTGAGGAGACGGTGGTGTCTCGCCCAAGGTTGAGTGCTTGGCAAGGTGCTTTTAGAAGGGCTTTGTTGGAAGGTGGTGTTGGTCCTGATAATGGGTTTGATTTGAGGCATCTTGTGGGCACTAAAACTGGTGGTTCTATCTTTGATGAGGATGGAAATAGACATGGAGCTGTGGAGCTTCTTAATGGGGTTGAACCCGGAAATCTTAAAGTTGCTGTTCGTGCTACTGTTCAGAAAATCATTTTTTCGGATCTATCAGCAATAGGAGTTTCATACTCCGATTCAGAAGGAAAGACACACACCGCATTCATCCGCAAGAAAGGGGAGATCATATTGAGCGCCGGAGCCATTGGAAGCCCTCAGCTTCTCCTTCTAAGTGGGGTTGGCCCAAAATCTCATCTTTCCTCCCTCAAACTCCCCGTCGTCCTCCACCAACCCCACGTCGGCGAATTCATGTCCGACAACCCCCGTTTCAGCGCCGCCGTCGTGCTTCCATTCCCACTGGTAGCTTCATCGGCACAAGTCGTCGGAATATTAGACGGCAATATCCATTTGCAAACATTTGCAAGCCCTGCACCATTTTTTATTTTACCAACTTTCGGCCTTCTTCCTCCTCAATCCACTCTGATCAACCCGAGCTTAGCCCTTCTTATCGGAAAATTCTCGGACGTGCTTTCAAAAGGCTCACTTCACCTGAATTCCTCGACAGACGTTAAGAACAACCCAATTGTTCGATTCAATTACTATACGAATCCTAACGATCTTGCACAATGCGTTAGAGGGTTACGTAAAGTGGGGGATTTTCTTAAAACCCGAACTATGGAAGAGATTAAGACGCAAGATTTGGAGGGTAATAAAGGGTTTCAGTTTCTAGGGCCTCCATTGCCGGAAAACTTGTCGGATAATAGCTCCGTCCAAGAGTATTGTAAGGAAACGGTGACCACTTATTGGCATTACCACGGCGGATGTTTGGTCGGGAAAGTTGTCGATCGTAATTACAGAGTCATCGGAATCAAAAATTTGCGTGTCGTAGATGGCTCGACTTTCTCTGTCTCGCCGGGGACTAATCCGATGGCCACCCTCATGATGCTTGGCCGGTATGTTGGCCTTAAGATGCTGCAAGAAAGATCAAGTTAA

Protein sequence

MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIFSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFGLLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRGLRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHGGCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS

Homology

[Close]

BLAST of CmaCh12G010120 vs. ExPASy Swiss-Prot
Match: P52706 ((R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1)

HSP 1 Score: 541.6 bits (1394), Expect = 1.0e-152
Identity = 273/538 (50.74%), Postives = 367/538 (68.22%), Query Frame = 0


Query: 4   ILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAAT 63
           +L L ++ + +  V S  T  N D SY++  +DATD+     YDY+I+GGGT+GCPLAAT
Sbjct: 12  VLHLFVLLLQYSEVHSLATTSNHDFSYLRFAYDATDLELEGSYDYVIVGGGTSGCPLAAT 71

Query: 64  LSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLG 123
           LS K+ VL+LERGS P  YP+VL   G +    +EDDG+ P +RFVSE+G++ +RGRVLG
Sbjct: 72  LSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLG 131

Query: 124 GGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLE 183
           G SM+NAG Y+R +   + ++GVDWDM+LV K YEWVE+T+V +P    WQ     A LE
Sbjct: 132 GTSMINAGVYARANTSIYSASGVDWDMDLVNKTYEWVEDTIVFKPNYQPWQSVTGTAFLE 191

Query: 184 GGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIFSD 243
            GV P++GF L H  GT+  GS FD  G RH A ELLN     NL+V V A+V+KIIFS+
Sbjct: 192 AGVDPNHGFSLDHEAGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVEKIIFSN 251

Query: 244 ---LSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 303
              L+A GV Y DS G  H AF+R KGE+I+SAG IG+PQLLLLSGVGP+S+LSSL +PV
Sbjct: 252 APGLTATGVIYRDSNGTPHRAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLSSLNIPV 311

Query: 304 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 363
           VL  P+VG+F+ DNPR    ++ P P+  +   V+GI   N   Q   S  P F  P F 
Sbjct: 312 VLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLP-FTTPPFS 371

Query: 364 LLPPQS-TLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVR 423
             P  S  L N + A    K +  LS GSL L SS++V+ +P V+FNYY+NP DL+ CV 
Sbjct: 372 FFPSTSYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNPTDLSHCVS 431

Query: 424 GLRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYH 483
           G++K+G+ L T  ++  K +DL G +GF  LG PLP++ +D+++ + +C+E+V +YWHYH
Sbjct: 432 GMKKIGELLSTDALKPYKVEDLPGIEGFNILGIPLPKDQTDDAAFETFCRESVASYWHYH 491

Query: 484 GGCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           GGCLVGKV+D ++RV GI  LRVVDGSTF  +P ++P    +MLGRYVG+K+LQERS+
Sbjct: 492 GGCLVGKVLDGDFRVTGIDALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQERSA 546

[Close]

BLAST of CmaCh12G010120 vs. ExPASy Swiss-Prot
Match: P52707 ((R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1)

HSP 1 Score: 537.0 bits (1382), Expect = 2.5e-151
Identity = 269/539 (49.91%), Postives = 367/539 (68.09%), Query Frame = 0


Query: 4   ILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAAT 63
           +L + ++ + +  V S     + D SY+  V+DATD      YDYII+GGGTAGCPLAAT
Sbjct: 12  VLHIFVLHLQYSEVQSLANTSSHDFSYLSFVYDATDPELEGSYDYIIVGGGTAGCPLAAT 71

Query: 64  LSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLG 123
           LS+ +SVL+LERGS P +YP++L   G +    +EDDG+ P +RFVSE+G++ +RGRVLG
Sbjct: 72  LSANYSVLVLERGSLPTEYPNLLISDGFVYNLQQEDDGKTPVERFVSEDGIDNVRGRVLG 131

Query: 124 GGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLE 183
           G SM+NAG Y R +  FF   G++WDM+LV + YEWVE+T+V  P    WQ     A LE
Sbjct: 132 GTSMINAGVYVRANTSFFNQTGIEWDMDLVNQTYEWVEDTIVFEPDSQTWQTVIGTAYLE 191

Query: 184 GGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIFSD 243
            G+ P+NGF + HL GT+  GS FD +G RH + ELLN  +P NL+VAV+A V+KIIFS 
Sbjct: 192 AGILPNNGFSVDHLAGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVQAAVEKIIFSS 251

Query: 244 ----LSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLP 303
               ++AIGV Y+DS G TH AF+R +GE+ILSAG IGSPQLLLLSGVGP+S+L+SL + 
Sbjct: 252 NTSGVTAIGVIYTDSNGTTHQAFVRGEGEVILSAGPIGSPQLLLLSGVGPESYLTSLNIS 311

Query: 304 VVLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTF 363
           VV   P+VG+++ DNPR    ++ P P+ AS+  V+GI          + P   F  P F
Sbjct: 312 VVASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSDFYQCSISSLP---FDTPPF 371

Query: 364 GLLPPQS-TLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCV 423
              P  S  L N + A ++ K    LS G++ LNSS+DV+  P V+FNYY+N  DL+ CV
Sbjct: 372 SFFPTTSYPLPNQTFAHIVNKVPGPLSHGTVTLNSSSDVRVGPNVKFNYYSNLTDLSHCV 431

Query: 424 RGLRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHY 483
            G++K+G+ L T  +E  K +DL G  GF  LG PLPEN +D+++ + +C+E+V +YWHY
Sbjct: 432 SGMKKLGEVLSTDALEPYKVEDLPGIDGFNILGIPLPENQTDDAAFETFCRESVASYWHY 491

Query: 484 HGGCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           HGGCLVGKV+D  +RV GI  LRVVDGSTF  +P ++P    +MLGRY+G+++LQERS+
Sbjct: 492 HGGCLVGKVLDDGFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGIQILQERSA 547

[Close]

BLAST of CmaCh12G010120 vs. ExPASy Swiss-Prot
Match: Q945K2 ((R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1)

HSP 1 Score: 535.4 bits (1378), Expect = 7.3e-151
Identity = 272/544 (50.00%), Postives = 370/544 (68.01%), Query Frame = 0


Query: 1   MATILPLILISIFFLRVLSSH---TIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAG 60
           M+ IL ++ I +  L+    H   T  + D SY+   +DATD+     YDY+I+GGGT+G
Sbjct: 6   MSAILLVLYIFVLHLQYSEVHSLATTSDHDFSYLSFAYDATDLELEGSYDYVIVGGGTSG 65

Query: 61  CPLAATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEII 120
           CPLAATLS K+ VL+LERGS P  YP+VL   G +    +EDDG+ P +RFVSE+G++ +
Sbjct: 66  CPLAATLSEKYKVLVLERGSLPTAYPNVLTADGFVYNLQQEDDGKTPVERFVSEDGIDNV 125

Query: 121 RGRVLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAF 180
           RGRVLGG S++NAG Y+R +   + ++GVDWDM+LV + YEWVE+T+V +P   +WQ   
Sbjct: 126 RGRVLGGTSIINAGVYARANTSIYSASGVDWDMDLVNQTYEWVEDTIVYKPNSQSWQSVT 185

Query: 181 RRALLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQ 240
           + A LE GV P++GF L H  GT+  GS FD  G RH A ELLN     NL+V V A+V+
Sbjct: 186 KTAFLEAGVHPNHGFSLDHEEGTRITGSTFDNKGTRHAADELLNKGNSNNLRVGVHASVE 245

Query: 241 KIIFSD---LSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLS 300
           KIIFS+   L+A GV Y DS G  H AF+R KGE+I+SAG IG+PQLLLLSGVGP+S+LS
Sbjct: 246 KIIFSNAPGLTATGVIYRDSNGTPHQAFVRSKGEVIVSAGTIGTPQLLLLSGVGPESYLS 305

Query: 301 SLKLPVVLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFF 360
           SL +PVVL  P+VG+F+ DNPR    ++ P P+  +   V+GI   N   Q   S  P F
Sbjct: 306 SLNIPVVLSHPYVGQFLHDNPRNFINILPPNPIEPTIVTVLGI--SNDFYQCSFSSLP-F 365

Query: 361 ILPTFGLLPPQS-TLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPND 420
             P FG  P  S  L N + A    K +  LS GSL L SS++V+ +P V+FNYY+N  D
Sbjct: 366 TTPPFGFFPSASYPLPNSTFAHFASKVAGPLSYGSLTLKSSSNVRVSPNVKFNYYSNLTD 425

Query: 421 LAQCVRGLRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVT 480
           L+ CV G++K+G+ L T  ++  K +DL G +GF  LG PLP++ +D+++ + +C+E+V 
Sbjct: 426 LSHCVSGMKKIGELLSTDALKPYKVEDLPGVEGFNILGIPLPKDQTDDAAFETFCRESVA 485

Query: 481 TYWHYHGGCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQ 538
           +YWHYHGGCLVGKV+D ++RV GI  LRVVDGSTF  +P ++P    +MLGRYVG+K+LQ
Sbjct: 486 SYWHYHGGCLVGKVLDGDFRVTGINALRVVDGSTFPYTPASHPQGFYLMLGRYVGIKILQ 545

[Close]

BLAST of CmaCh12G010120 vs. ExPASy Swiss-Prot
Match: Q9SSM2 ((R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 SV=1)

HSP 1 Score: 527.3 bits (1357), Expect = 2.0e-148
Identity = 262/520 (50.38%), Postives = 359/520 (69.04%), Query Frame = 0


Query: 25  NQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPS 84
           N+   +M+ + +ATD    + YDYII+GGGTAGCPLAATLS  F VLLLERG  P   P+
Sbjct: 33  NRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPN 92

Query: 85  VLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLGGGSMVNAGFYSRGHREFFESA 144
           V++  G L    + ++ ++P Q F+SE GV   RGRVLGG S +NAGFYSR  ++FFE++
Sbjct: 93  VMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENS 152

Query: 145 GVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLEGGVGPDNGFDLRHLVGTKTGG 204
           G+ WD+  V + YEWVE  +V RP+L  WQ A R ALLE GV P NGF L H VGTK GG
Sbjct: 153 GLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGG 212

Query: 205 SIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIF--------SDLSAIGVSYSDSEG 264
           S FD  G RH + +LL      N++VAV ATV++++         S++SAIGV Y D  G
Sbjct: 213 STFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLG 272

Query: 265 KTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGEFMSDNPR 324
           + H A IR +GE+ILSAGA+GSPQLL LSG+GP+S+LS+  +PV L QPHVG+F+ DNPR
Sbjct: 273 RFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPR 332

Query: 325 FSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFI-LPTFGLLPPQSTLINPSLAL 384
              ++V P P+  S  QVVG+ +    L+  ++  PF   L +  +  P S L  P +  
Sbjct: 333 NGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVP-VTT 392

Query: 385 LIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRGLRKVGDFLKTRTMEE 444
           ++ K    +S G L L +STDV+ NP+VRFNY+++P DL +CV G RK+G+ L++R M++
Sbjct: 393 IMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQD 452

Query: 445 IKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHGGCLVGKVVDRNYRVI 504
              ++  GN+ F+F+G PLP + S++  + ++C+ TV+T WHYHGG +VGKVVD + +VI
Sbjct: 453 FMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVI 512

Query: 505 GIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQER 536
           G+ +LR+VDGSTF++SPGTNP ATLMMLGRY+GLKML+ER
Sbjct: 513 GVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

[Close]

BLAST of CmaCh12G010120 vs. ExPASy Swiss-Prot
Match: O50048 ((R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.6e-148
Identity = 268/536 (50.00%), Postives = 361/536 (67.35%), Query Frame = 0


Query: 5   LPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAATL 64
           L L ++ + +  V S       D SY++ V+DA D      YDYII+GGGTAGCPLAATL
Sbjct: 14  LHLFVLHLQYSEVQSLANTSAHDFSYLEFVYDANDTELEGTYDYIIVGGGTAGCPLAATL 73

Query: 65  SSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLGG 124
           S+ +SVL+LERG+ P +YP++L   G +    +EDDG+ P +RFVS +G++ +RGRVLGG
Sbjct: 74  SANYSVLVLERGTLPTEYPNLLTSDGFIYNLQQEDDGQTPVERFVSGDGIDNVRGRVLGG 133

Query: 125 GSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLEG 184
            SM+NAG Y R +  FF   G++WDM+LV K Y+WVE+T+V +P    WQ     A LE 
Sbjct: 134 TSMINAGVYVRANTSFFNQTGIEWDMDLVNKTYDWVEDTIVFKPDFQFWQNLTGTAFLEV 193

Query: 185 GVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIFSD- 244
           G+ PDNGF L HL GT+  GS FD +G RH + ELLN  +P NL+VAV A V+KIIFS  
Sbjct: 194 GILPDNGFSLDHLEGTRLTGSTFDNNGTRHASDELLNKGDPNNLRVAVHAAVEKIIFSSD 253

Query: 245 ---LSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 304
              ++AIGV Y+DS G TH AF+R  GE+ILSAG IGSPQLLLLSGVG +S+L+SL + V
Sbjct: 254 SSGVTAIGVIYTDSNGTTHQAFVRGDGEVILSAGPIGSPQLLLLSGVGLESYLTSLNISV 313

Query: 305 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 364
           V   P+VG+++ DNPR    ++ P P+ AS+  V+GI       Q   S  PF   P FG
Sbjct: 314 VASHPYVGQYIYDNPRNFINILPPNPIEASTVTVLGITSD--FYQCSISSLPFSTAP-FG 373

Query: 365 LLP-PQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVR 424
             P P   L N + A ++ K    LS G++ L S++DV+  P V FNYY+N  DLA CV 
Sbjct: 374 FFPNPTYPLPNTTFAHIVNKVPGPLSHGTVLLQSTSDVRVAPNVTFNYYSNTTDLAHCVS 433

Query: 425 GLRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYH 484
           G++K+G+FL +  ++  K +DL G +GF  LG PLPEN +D+++ + +C+E V +YWHYH
Sbjct: 434 GMKKIGEFLSSDALKPYKVEDLPGIEGFDILGIPLPENQTDDAAFETFCREAVASYWHYH 493

Query: 485 GGCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQER 536
           GGCLVG+V+D ++RV GI  LRVVDGSTF  +P ++P    +MLGRY+G K+LQER
Sbjct: 494 GGCLVGEVLDDDFRVTGINALRVVDGSTFPSTPASHPQGFYLMLGRYMGTKILQER 546

[Close]

BLAST of CmaCh12G010120 vs. ExPASy TrEMBL
Match: A0A6J1HRM5 ((R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465498 PE=3 SV=1)

HSP 1 Score: 1073.5 bits (2775), Expect = 2.0e-310
Identity = 537/537 (100.00%), Postives = 537/537 (100.00%), Query Frame = 0


Query: 1   MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL 60
           MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL
Sbjct: 1   MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL 60

Query: 61  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR 120
           AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR
Sbjct: 61  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR 120

Query: 121 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA 180
           VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA
Sbjct: 121 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA 180

Query: 181 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 240
           LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII
Sbjct: 181 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 240

Query: 241 FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 300
           FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV
Sbjct: 241 FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 300

Query: 301 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 360
           VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG
Sbjct: 301 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 360

Query: 361 LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG 420
           LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG
Sbjct: 361 LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG 420

Query: 421 LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG 480
           LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG
Sbjct: 421 LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG 480

Query: 481 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS
Sbjct: 481 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 537

[Close]

BLAST of CmaCh12G010120 vs. ExPASy TrEMBL
Match: A0A6J1FC90 ((R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444087 PE=3 SV=1)

HSP 1 Score: 1032.3 bits (2668), Expect = 7.0e-298
Identity = 514/537 (95.72%), Postives = 529/537 (98.51%), Query Frame = 0


Query: 1   MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL 60
           MATILPLILISIF LRVLSS+TIPNQDVSYMKLVHDAT++PENE+YDYIIIGGGTAGCPL
Sbjct: 8   MATILPLILISIFLLRVLSSNTIPNQDVSYMKLVHDATELPENEDYDYIIIGGGTAGCPL 67

Query: 61  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR 120
           AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSE+GVEIIRGR
Sbjct: 68  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSEDGVEIIRGR 127

Query: 121 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA 180
           VLGGGSMVNAGFYSRGH EFFESAGVDWDMELVEKGYEWVE+TVVSRPRLSAWQ AFRRA
Sbjct: 128 VLGGGSMVNAGFYSRGHGEFFESAGVDWDMELVEKGYEWVEKTVVSRPRLSAWQSAFRRA 187

Query: 181 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 240
           LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII
Sbjct: 188 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 247

Query: 241 FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 300
           FSDLSAIGVSYSDS+GKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV
Sbjct: 248 FSDLSAIGVSYSDSKGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 307

Query: 301 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 360
           VLHQPHVGEFMSDNPRFSAA+VLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG
Sbjct: 308 VLHQPHVGEFMSDNPRFSAAIVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 367

Query: 361 LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG 420
           LLPPQSTLINPSLALLIGKF+DVLSKGSLHLNSSTDVKNNPIVRFNYY+NPNDLAQCVRG
Sbjct: 368 LLPPQSTLINPSLALLIGKFTDVLSKGSLHLNSSTDVKNNPIVRFNYYSNPNDLAQCVRG 427

Query: 421 LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG 480
           LRKVGD LKTR ME+IKT+DLEGNKGF FLGPPLPENLSD SSV+EYCKETVTTYWHYHG
Sbjct: 428 LRKVGDLLKTRMMEKIKTRDLEGNKGFLFLGPPLPENLSDISSVEEYCKETVTTYWHYHG 487

Query: 481 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           GCLVGKVVD NYRVIG++NLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS
Sbjct: 488 GCLVGKVVDGNYRVIGVENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 544

[Close]

BLAST of CmaCh12G010120 vs. ExPASy TrEMBL
Match: A0A6J1G4V5 ((R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111450816 PE=3 SV=1)

HSP 1 Score: 944.9 bits (2441), Expect = 1.5e-271
Identity = 464/537 (86.41%), Postives = 504/537 (93.85%), Query Frame = 0


Query: 1   MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL 60
           MA  LPLI IS F +RVLSSH I NQD SYMKLVHDATD+PENEEYDYIIIGGGTAGCPL
Sbjct: 28  MAITLPLIFISFFLVRVLSSHAISNQDESYMKLVHDATDLPENEEYDYIIIGGGTAGCPL 87

Query: 61  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR 120
           AATLSSK+S+L+LERGS+PNKYPSVL E+GLLNVF EEDDG+NPFQRF++ +GV++IRGR
Sbjct: 88  AATLSSKYSILVLERGSEPNKYPSVLKEEGLLNVFAEEDDGQNPFQRFITVDGVDVIRGR 147

Query: 121 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA 180
           VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVE+ YEWVE+TVVSRPRLS WQ AFR A
Sbjct: 148 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEEAYEWVEQTVVSRPRLSDWQSAFRSA 207

Query: 181 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 240
           LLEGGV P NGFDLRHLVGTK GGSIFD +GNRHGAVELLNGV+P NLKVAVRATV+KII
Sbjct: 208 LLEGGVRPYNGFDLRHLVGTKIGGSIFDVEGNRHGAVELLNGVQPENLKVAVRATVEKII 267

Query: 241 FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 300
           FSDLSAIGVSYSDS+G THTAFIRKKGEIILSAGAIGSPQLLLLSGVG KSHLSSLKLPV
Sbjct: 268 FSDLSAIGVSYSDSKGNTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGSKSHLSSLKLPV 327

Query: 301 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 360
           VL QP VGEFMSDNPRF+AA+VLPFPLVASS+QVVGILDGNI+L+T ASP+PFFI PTF 
Sbjct: 328 VLDQPDVGEFMSDNPRFTAAIVLPFPLVASSSQVVGILDGNIYLETLASPSPFFISPTFS 387

Query: 361 LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG 420
           LLPPQST+INPSLAL IGKFS+VLSKGSLHLNSST+V N+PIVRFNYY+NPNDL +CVRG
Sbjct: 388 LLPPQSTMINPSLALFIGKFSNVLSKGSLHLNSSTNVNNDPIVRFNYYSNPNDLVRCVRG 447

Query: 421 LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG 480
           LRKVGD LKTRTME+IKTQDL+GNK FQFLGPPLPENLSDNSSV+EYCKETVTTYWHYHG
Sbjct: 448 LRKVGDLLKTRTMEKIKTQDLKGNKRFQFLGPPLPENLSDNSSVEEYCKETVTTYWHYHG 507

Query: 481 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           GCLVGKVVD NYRVIG++NLRVVDGSTF+VSPGTNPMAT+MMLGRYVGL+MLQ+RSS
Sbjct: 508 GCLVGKVVDSNYRVIGVENLRVVDGSTFAVSPGTNPMATVMMLGRYVGLQMLQQRSS 564

[Close]

BLAST of CmaCh12G010120 vs. ExPASy TrEMBL
Match: A0A5D3CL00 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold231G00890 PE=3 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 4.2e-250
Identity = 433/534 (81.09%), Postives = 478/534 (89.51%), Query Frame = 0


Query: 4   ILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAAT 63
           IL   LISIF L V+SSH IPNQD SYMK VH+A+++ E+EEYDYIIIGGGTAGCPLAAT
Sbjct: 5   ILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAAT 64

Query: 64  LSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLG 123
           LSSKFSVLLLERG+DPNKYPSVLNEQGLLN FV EDDG+NPFQ F+SE+GVE +RGRVLG
Sbjct: 65  LSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLG 124

Query: 124 GGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLE 183
           GGSM+NAGFYSRGHREFFE+AGVDWDMELVEK YEWVEETVVS+P LS WQ AFR ALLE
Sbjct: 125 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLE 184

Query: 184 GGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIFSD 243
           GGVG DNGFDL+HLVGTKTGGSIFD  GNRHGAVELLN  EP N KVA +ATVQ+IIF+ 
Sbjct: 185 GGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTG 244

Query: 244 LSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLH 303
           LSA GVSYSDS+GK HTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKS L SLKLPVVL 
Sbjct: 245 LSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLD 304

Query: 304 QPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFGLLP 363
           QPHVGEFMSDNPRF+  +VLPF +VASSAQVVG LD NIHLQ FASP PFF  P+F LLP
Sbjct: 305 QPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLP 364

Query: 364 PQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRGLRK 423
           PQST I PSLA+ +GKFSDV S+GSL LNSS DVK +PIVRFNYY++P+DLA+CVRG+RK
Sbjct: 365 PQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRK 424

Query: 424 VGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHGGCL 483
           VGD L+T TME+IKTQDLEGNK FQFLG  LPENL ++S+V+EYC++TVTTYWHYHGGCL
Sbjct: 425 VGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCL 484

Query: 484 VGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           VGKVVD N++VIGI+NLRVVDGSTFSVSPGTNPMATLMMLGRYVGLK+LQ+RSS
Sbjct: 485 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQRSS 538

[Close]

BLAST of CmaCh12G010120 vs. ExPASy TrEMBL
Match: A0A1S3BSP4 ((R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=1)

HSP 1 Score: 873.6 bits (2256), Expect = 4.2e-250
Identity = 433/534 (81.09%), Postives = 478/534 (89.51%), Query Frame = 0


Query: 4   ILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAAT 63
           IL   LISIF L V+SSH IPNQD SYMK VH+A+++ E+EEYDYIIIGGGTAGCPLAAT
Sbjct: 5   ILLFTLISIFQLGVISSHPIPNQDTSYMKFVHEASELQESEEYDYIIIGGGTAGCPLAAT 64

Query: 64  LSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLG 123
           LSSKFSVLLLERG+DPNKYPSVLNEQGLLN FV EDDG+NPFQ F+SE+GVE +RGRVLG
Sbjct: 65  LSSKFSVLLLERGNDPNKYPSVLNEQGLLNAFVAEDDGQNPFQHFISEDGVENLRGRVLG 124

Query: 124 GGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLE 183
           GGSM+NAGFYSRGHREFFE+AGVDWDMELVEK YEWVEETVVS+P LS WQ AFR ALLE
Sbjct: 125 GGSMINAGFYSRGHREFFETAGVDWDMELVEKAYEWVEETVVSKPSLSPWQAAFRSALLE 184

Query: 184 GGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIFSD 243
           GGVG DNGFDL+HLVGTKTGGSIFD  GNRHGAVELLN  EP N KVA +ATVQ+IIF+ 
Sbjct: 185 GGVGHDNGFDLKHLVGTKTGGSIFDNKGNRHGAVELLNKGEPENFKVATQATVQRIIFTG 244

Query: 244 LSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLH 303
           LSA GVSYSDS+GK HTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKS L SLKLPVVL 
Sbjct: 245 LSASGVSYSDSKGKLHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSQLKSLKLPVVLD 304

Query: 304 QPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFGLLP 363
           QPHVGEFMSDNPRF+  +VLPF +VASSAQVVG LD NIHLQ FASP PFF  P+F LLP
Sbjct: 305 QPHVGEFMSDNPRFTPTIVLPFQVVASSAQVVGTLDNNIHLQAFASPLPFFAPPSFSLLP 364

Query: 364 PQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRGLRK 423
           PQST I PSLA+ +GKFSDV S+GSL LNSS DVK +PIVRFNYY++P+DLA+CVRG+RK
Sbjct: 365 PQSTSIVPSLAIFVGKFSDVYSEGSLRLNSSIDVKESPIVRFNYYSHPDDLARCVRGVRK 424

Query: 424 VGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHGGCL 483
           VGD L+T TME+IKTQDLEGNK FQFLG  LPENL ++S+V+EYC++TVTTYWHYHGGCL
Sbjct: 425 VGDLLRTPTMEKIKTQDLEGNKRFQFLGLSLPENLLNDSAVEEYCQKTVTTYWHYHGGCL 484

Query: 484 VGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           VGKVVD N++VIGI+NLRVVDGSTFSVSPGTNPMATLMMLGRYVGLK+LQ+RSS
Sbjct: 485 VGKVVDDNHKVIGIENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKVLQQRSS 538

[Close]

BLAST of CmaCh12G010120 vs. NCBI nr
Match: XP_022965669.1 ((R)-mandelonitrile lyase 1-like [Cucurbita maxima])

HSP 1 Score: 1073.5 bits (2775), Expect = 4.1e-310
Identity = 537/537 (100.00%), Postives = 537/537 (100.00%), Query Frame = 0


Query: 1   MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL 60
           MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL
Sbjct: 1   MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL 60

Query: 61  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR 120
           AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR
Sbjct: 61  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR 120

Query: 121 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA 180
           VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA
Sbjct: 121 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA 180

Query: 181 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 240
           LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII
Sbjct: 181 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 240

Query: 241 FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 300
           FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV
Sbjct: 241 FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 300

Query: 301 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 360
           VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG
Sbjct: 301 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 360

Query: 361 LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG 420
           LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG
Sbjct: 361 LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG 420

Query: 421 LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG 480
           LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG
Sbjct: 421 LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG 480

Query: 481 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS
Sbjct: 481 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 537

[Close]

BLAST of CmaCh12G010120 vs. NCBI nr
Match: XP_022937802.1 ((R)-mandelonitrile lyase 1-like [Cucurbita moschata])

HSP 1 Score: 1032.3 bits (2668), Expect = 1.5e-297
Identity = 514/537 (95.72%), Postives = 529/537 (98.51%), Query Frame = 0


Query: 1   MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL 60
           MATILPLILISIF LRVLSS+TIPNQDVSYMKLVHDAT++PENE+YDYIIIGGGTAGCPL
Sbjct: 8   MATILPLILISIFLLRVLSSNTIPNQDVSYMKLVHDATELPENEDYDYIIIGGGTAGCPL 67

Query: 61  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR 120
           AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSE+GVEIIRGR
Sbjct: 68  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSEDGVEIIRGR 127

Query: 121 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA 180
           VLGGGSMVNAGFYSRGH EFFESAGVDWDMELVEKGYEWVE+TVVSRPRLSAWQ AFRRA
Sbjct: 128 VLGGGSMVNAGFYSRGHGEFFESAGVDWDMELVEKGYEWVEKTVVSRPRLSAWQSAFRRA 187

Query: 181 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 240
           LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII
Sbjct: 188 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 247

Query: 241 FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 300
           FSDLSAIGVSYSDS+GKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV
Sbjct: 248 FSDLSAIGVSYSDSKGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 307

Query: 301 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 360
           VLHQPHVGEFMSDNPRFSAA+VLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG
Sbjct: 308 VLHQPHVGEFMSDNPRFSAAIVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 367

Query: 361 LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG 420
           LLPPQSTLINPSLALLIGKF+DVLSKGSLHLNSSTDVKNNPIVRFNYY+NPNDLAQCVRG
Sbjct: 368 LLPPQSTLINPSLALLIGKFTDVLSKGSLHLNSSTDVKNNPIVRFNYYSNPNDLAQCVRG 427

Query: 421 LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG 480
           LRKVGD LKTR ME+IKT+DLEGNKGF FLGPPLPENLSD SSV+EYCKETVTTYWHYHG
Sbjct: 428 LRKVGDLLKTRMMEKIKTRDLEGNKGFLFLGPPLPENLSDISSVEEYCKETVTTYWHYHG 487

Query: 481 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           GCLVGKVVD NYRVIG++NLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS
Sbjct: 488 GCLVGKVVDGNYRVIGVENLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 544

[Close]

BLAST of CmaCh12G010120 vs. NCBI nr
Match: XP_023546454.1 ((R)-mandelonitrile lyase 1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 955.3 bits (2468), Expect = 2.3e-274
Identity = 468/537 (87.15%), Postives = 509/537 (94.79%), Query Frame = 0


Query: 1   MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL 60
           MA  LPLI IS F +RVLSSH+IPN+D SYMKLVHDATD+PENEEYDYIIIGGGTAGCPL
Sbjct: 31  MAITLPLIFISFFLVRVLSSHSIPNRDESYMKLVHDATDLPENEEYDYIIIGGGTAGCPL 90

Query: 61  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR 120
           AATLSSK+SVL+LERGS+PNKYPSVL E+GL+NVF EEDDG+NPFQRF++E+GV++IRGR
Sbjct: 91  AATLSSKYSVLVLERGSEPNKYPSVLKEEGLMNVFAEEDDGQNPFQRFITEDGVDVIRGR 150

Query: 121 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA 180
           VLGGGSMVNAGFYSRGHREFFESAGVDWDME+VE+ YEWVE+TVVSRPRLS WQ AFR A
Sbjct: 151 VLGGGSMVNAGFYSRGHREFFESAGVDWDMEMVEEAYEWVEQTVVSRPRLSDWQSAFRSA 210

Query: 181 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 240
           LLEGGV P NGFDLRHLVGTK GGSIFDE+G+RHGAVELLNGVEP NLKVAVRATV+KII
Sbjct: 211 LLEGGVRPYNGFDLRHLVGTKIGGSIFDEEGSRHGAVELLNGVEPENLKVAVRATVEKII 270

Query: 241 FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 300
           FSDLSAIGVSYSDS+G THTAFIRKKGEIILSAGAIGSPQLLLLSGVG KSHLSSLKLPV
Sbjct: 271 FSDLSAIGVSYSDSKGNTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGSKSHLSSLKLPV 330

Query: 301 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 360
           VL QP VGEFMSDNPRF+AA+VLPFPLVASS+QVVGILDGNI+L+TFASP+PFFI PTF 
Sbjct: 331 VLDQPDVGEFMSDNPRFTAAIVLPFPLVASSSQVVGILDGNIYLETFASPSPFFISPTFS 390

Query: 361 LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG 420
           LLPPQST+INPSLAL IGKFS+VLSKGSLHLNSST+V N+PIVRFNYY+NPNDL QCVRG
Sbjct: 391 LLPPQSTMINPSLALFIGKFSNVLSKGSLHLNSSTNVNNDPIVRFNYYSNPNDLVQCVRG 450

Query: 421 LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG 480
           LRKVGD LKTRTME+IKTQDL+GNK FQFLGPPLPENLSDNSSV+EYCKETVTTYWHYHG
Sbjct: 451 LRKVGDLLKTRTMEKIKTQDLKGNKRFQFLGPPLPENLSDNSSVEEYCKETVTTYWHYHG 510

Query: 481 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           GCLVGKVVD NYRVIG++NLRVVDGSTFSVSPGTNPMAT+MMLGRYVGL+MLQ+RSS
Sbjct: 511 GCLVGKVVDSNYRVIGVQNLRVVDGSTFSVSPGTNPMATVMMLGRYVGLQMLQQRSS 567

[Close]

BLAST of CmaCh12G010120 vs. NCBI nr
Match: XP_022946877.1 ((R)-mandelonitrile lyase 1-like [Cucurbita moschata])

HSP 1 Score: 944.9 bits (2441), Expect = 3.0e-271
Identity = 464/537 (86.41%), Postives = 504/537 (93.85%), Query Frame = 0


Query: 1   MATILPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPL 60
           MA  LPLI IS F +RVLSSH I NQD SYMKLVHDATD+PENEEYDYIIIGGGTAGCPL
Sbjct: 28  MAITLPLIFISFFLVRVLSSHAISNQDESYMKLVHDATDLPENEEYDYIIIGGGTAGCPL 87

Query: 61  AATLSSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGR 120
           AATLSSK+S+L+LERGS+PNKYPSVL E+GLLNVF EEDDG+NPFQRF++ +GV++IRGR
Sbjct: 88  AATLSSKYSILVLERGSEPNKYPSVLKEEGLLNVFAEEDDGQNPFQRFITVDGVDVIRGR 147

Query: 121 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRA 180
           VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVE+ YEWVE+TVVSRPRLS WQ AFR A
Sbjct: 148 VLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEEAYEWVEQTVVSRPRLSDWQSAFRSA 207

Query: 181 LLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKII 240
           LLEGGV P NGFDLRHLVGTK GGSIFD +GNRHGAVELLNGV+P NLKVAVRATV+KII
Sbjct: 208 LLEGGVRPYNGFDLRHLVGTKIGGSIFDVEGNRHGAVELLNGVQPENLKVAVRATVEKII 267

Query: 241 FSDLSAIGVSYSDSEGKTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPV 300
           FSDLSAIGVSYSDS+G THTAFIRKKGEIILSAGAIGSPQLLLLSGVG KSHLSSLKLPV
Sbjct: 268 FSDLSAIGVSYSDSKGNTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGSKSHLSSLKLPV 327

Query: 301 VLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILPTFG 360
           VL QP VGEFMSDNPRF+AA+VLPFPLVASS+QVVGILDGNI+L+T ASP+PFFI PTF 
Sbjct: 328 VLDQPDVGEFMSDNPRFTAAIVLPFPLVASSSQVVGILDGNIYLETLASPSPFFISPTFS 387

Query: 361 LLPPQSTLINPSLALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRG 420
           LLPPQST+INPSLAL IGKFS+VLSKGSLHLNSST+V N+PIVRFNYY+NPNDL +CVRG
Sbjct: 388 LLPPQSTMINPSLALFIGKFSNVLSKGSLHLNSSTNVNNDPIVRFNYYSNPNDLVRCVRG 447

Query: 421 LRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHG 480
           LRKVGD LKTRTME+IKTQDL+GNK FQFLGPPLPENLSDNSSV+EYCKETVTTYWHYHG
Sbjct: 448 LRKVGDLLKTRTMEKIKTQDLKGNKRFQFLGPPLPENLSDNSSVEEYCKETVTTYWHYHG 507

Query: 481 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQERSS 538
           GCLVGKVVD NYRVIG++NLRVVDGSTF+VSPGTNPMAT+MMLGRYVGL+MLQ+RSS
Sbjct: 508 GCLVGKVVDSNYRVIGVENLRVVDGSTFAVSPGTNPMATVMMLGRYVGLQMLQQRSS 564

[Close]

BLAST of CmaCh12G010120 vs. NCBI nr
Match: KAG7030241.1 ((R)-mandelonitrile lyase 1, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 914.1 bits (2361), Expect = 5.8e-262
Identity = 445/507 (87.77%), Postives = 484/507 (95.46%), Query Frame = 0


Query: 31  MKLVHDATDIPENEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPSVLNEQG 90
           MKLVHDATD+PENEEYDYIIIGGGTAGCPLAATLSSK+SVL+LERGS+PNKYPSVL E+G
Sbjct: 1   MKLVHDATDLPENEEYDYIIIGGGTAGCPLAATLSSKYSVLVLERGSEPNKYPSVLKEEG 60

Query: 91  LLNVFVEEDDGENPFQRFVSENGVEIIRGRVLGGGSMVNAGFYSRGHREFFESAGVDWDM 150
           L+NVF EEDDG+NPFQRF++E+GV++IRGRVLGGGSMVNAGFYSRGHREFFESAGVDWDM
Sbjct: 61  LMNVFAEEDDGQNPFQRFMTEDGVDVIRGRVLGGGSMVNAGFYSRGHREFFESAGVDWDM 120

Query: 151 ELVEKGYEWVEETVVSRPRLSAWQGAFRRALLEGGVGPDNGFDLRHLVGTKTGGSIFDED 210
           ELVE+ YEWVE+TVVSRPRLS WQ AFR ALLEGGV P NGFDLRHLVGTK GGSIFDE+
Sbjct: 121 ELVEEAYEWVEQTVVSRPRLSDWQSAFRSALLEGGVRPYNGFDLRHLVGTKIGGSIFDEE 180

Query: 211 GNRHGAVELLNGVEPGNLKVAVRATVQKIIFSDLSAIGVSYSDSEGKTHTAFIRKKGEII 270
           G+RHGAVELLNGVEP NLKVAVRATV+KIIFSDLSAIGVSYSDS+G THTAFIRKKGEII
Sbjct: 181 GSRHGAVELLNGVEPENLKVAVRATVEKIIFSDLSAIGVSYSDSKGNTHTAFIRKKGEII 240

Query: 271 LSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGEFMSDNPRFSAAVVLPFPLVAS 330
           LSAGAIGSPQLLLLSGVG KSHLSSLKLPVVL QP VGEFMSDNPRF+AA+VLPFPLVAS
Sbjct: 241 LSAGAIGSPQLLLLSGVGSKSHLSSLKLPVVLDQPDVGEFMSDNPRFTAAIVLPFPLVAS 300

Query: 331 SAQVVGILDGNIHLQTFASPAPFFILPTFGLLPPQSTLINPSLALLIGKFSDVLSKGSLH 390
           S+QVVGILDGNI+L+T ASP+PFFI PTF LLPPQST+INPSLAL IGKFS+VLSKGSLH
Sbjct: 301 SSQVVGILDGNIYLETLASPSPFFISPTFSLLPPQSTMINPSLALFIGKFSNVLSKGSLH 360

Query: 391 LNSSTDVKNNPIVRFNYYTNPNDLAQCVRGLRKVGDFLKTRTMEEIKTQDLEGNKGFQFL 450
           LNSST+V N+PIVRFNYY+NPNDL +CVRGLRKVGD LKTRTME+IKTQDL+GNK FQFL
Sbjct: 361 LNSSTNVNNDPIVRFNYYSNPNDLVRCVRGLRKVGDLLKTRTMEKIKTQDLKGNKRFQFL 420

Query: 451 GPPLPENLSDNSSVQEYCKETVTTYWHYHGGCLVGKVVDRNYRVIGIKNLRVVDGSTFSV 510
           GPPLPENLSDNSSV+EYCKETVTTYWHYHGGCLVGKVVD NYRVIG++NLRVVDGSTF+V
Sbjct: 421 GPPLPENLSDNSSVEEYCKETVTTYWHYHGGCLVGKVVDSNYRVIGVENLRVVDGSTFAV 480

Query: 511 SPGTNPMATLMMLGRYVGLKMLQERSS 538
           SPGTNPMAT+MMLGRYVGL+MLQ+RSS
Sbjct: 481 SPGTNPMATVMMLGRYVGLQMLQQRSS 507

[Close]

BLAST of CmaCh12G010120 vs. TAIR 10
Match: AT1G73050.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 527.3 bits (1357), Expect = 1.4e-149
Identity = 262/520 (50.38%), Postives = 359/520 (69.04%), Query Frame = 0


Query: 25  NQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSDPNKYPS 84
           N+   +M+ + +ATD    + YDYII+GGGTAGCPLAATLS  F VLLLERG  P   P+
Sbjct: 33  NRPPGFMRFISNATDFASEDYYDYIIVGGGTAGCPLAATLSQSFRVLLLERGGVPYNRPN 92

Query: 85  VLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLGGGSMVNAGFYSRGHREFFESA 144
           V++  G L    + ++ ++P Q F+SE GV   RGRVLGG S +NAGFYSR  ++FFE++
Sbjct: 93  VMSHDGFLTTLTDVNNFDSPAQSFISEEGVPNARGRVLGGSSAINAGFYSRADKQFFENS 152

Query: 145 GVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLEGGVGPDNGFDLRHLVGTKTGG 204
           G+ WD+  V + YEWVE  +V RP+L  WQ A R ALLE GV P NGF L H VGTK GG
Sbjct: 153 GLVWDLSSVNQSYEWVERAIVFRPQLRTWQTAIRDALLEVGVHPFNGFTLEHKVGTKIGG 212

Query: 205 SIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIF--------SDLSAIGVSYSDSEG 264
           S FD  G RH + +LL      N++VAV ATV++++         S++SAIGV Y D  G
Sbjct: 213 STFDRTGRRHSSADLLRYARSSNIRVAVYATVERVLLASSPSVSGSNVSAIGVVYRDQLG 272

Query: 265 KTHTAFIRKKGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGEFMSDNPR 324
           + H A IR +GE+ILSAGA+GSPQLL LSG+GP+S+LS+  +PV L QPHVG+F+ DNPR
Sbjct: 273 RFHHALIRDRGEVILSAGALGSPQLLFLSGIGPRSYLSTWGIPVALDQPHVGDFVYDNPR 332

Query: 325 FSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFI-LPTFGLLPPQSTLINPSLAL 384
              ++V P P+  S  QVVG+ +    L+  ++  PF   L +  +  P S L  P +  
Sbjct: 333 NGISIVPPVPMENSLIQVVGVTEDGAFLEAASNVIPFASPLHSVFIRAPASPLYVP-VTT 392

Query: 385 LIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRGLRKVGDFLKTRTMEE 444
           ++ K    +S G L L +STDV+ NP+VRFNY+++P DL +CV G RK+G+ L++R M++
Sbjct: 393 IMEKILGPVSIGLLRL-ASTDVRINPVVRFNYFSDPQDLERCVNGTRKIGEILRSRAMQD 452

Query: 445 IKTQDLEGNKGFQFLGPPLPENLSDNSSVQEYCKETVTTYWHYHGGCLVGKVVDRNYRVI 504
              ++  GN+ F+F+G PLP + S++  + ++C+ TV+T WHYHGG +VGKVVD + +VI
Sbjct: 453 FMIREWFGNRRFRFVGAPLPVDQSNDLVMADFCRRTVSTIWHYHGGAVVGKVVDSDLKVI 512

Query: 505 GIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQER 536
           G+ +LR+VDGSTF++SPGTNP ATLMMLGRY+GLKML+ER
Sbjct: 513 GVNSLRLVDGSTFNISPGTNPQATLMMLGRYMGLKMLRER 550

[Close]

BLAST of CmaCh12G010120 vs. TAIR 10
Match: AT1G12570.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 405.6 bits (1041), Expect = 6.2e-113
Identity = 237/560 (42.32%), Postives = 321/560 (57.32%), Query Frame = 0


Query: 17  VLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERG 76
           + SS   PN   S+M+   DAT  P    YDYIIIGGGTAGCPLAATLS   SVLLLERG
Sbjct: 22  ICSSDKAPN--YSFMR---DATGSPTTSYYDYIIIGGGTAGCPLAATLSQNASVLLLERG 81

Query: 77  SDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLGGGSMVNAGFYSRG 136
             P   P++          + +    +P QRFVSE+GV   R RVLGGGS +NAGFY+R 
Sbjct: 82  DSPYNNPNI-TRLSAFGAALSDLSESSPSQRFVSEDGVINARARVLGGGSALNAGFYTRA 141

Query: 137 HREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLEGGVGPDNGFDLRH 196
             ++  + G  WD  L  + Y+WVE  V  +P +  WQ A R  LLE G+ P+NGF   H
Sbjct: 142 GTKYVRNMG--WDGALANESYQWVEAKVAFQPPMGRWQTAVRDGLLEAGIVPNNGFTYDH 201

Query: 197 LVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIFSDLS-----AIGVSY 256
           + GTK GG+IFD +GNRH A +LL   +P  + V + ATV +I+F         A GV Y
Sbjct: 202 INGTKFGGTIFDRNGNRHTAADLLEYADPKGITVLLHATVHRILFRTRGTTKPIANGVVY 261

Query: 257 SDSEGKTHTAFIRK--KGEIILSAGAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGE 316
            D  G+ H A++++    EIILSAG +GSPQLL+LSGVGP + L +  + VV+ QPHVG+
Sbjct: 262 RDRTGQAHRAYLKEGALSEIILSAGTLGSPQLLMLSGVGPSAQLQAQNITVVMDQPHVGQ 321

Query: 317 FMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTF----------ASPAPFFILPTF 376
            M DNP  +  V  P P+  S  +VVGI     +++             S         +
Sbjct: 322 GMYDNPMNAVFVPSPVPVEVSLIEVVGITGEGTYVEAAGGENFGGGGGGSSGSSSTRDYY 381

Query: 377 GLLPPQSTLINPSL------------ALLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNY 436
            +  P++TL+  +               L+ K    LS G L L  + + K+NPIV FNY
Sbjct: 382 AMFSPRATLLESNSMTKLSSAQPFQGGFLLEKVMGPLSTGHLEL-KTRNPKDNPIVTFNY 441

Query: 437 YTNPNDLAQCVRGLRKVGDFLKTRTMEEIKTQDLEGNKGFQF---LGPPLPENL------ 496
           + +P+DL +CVRG++ +   ++++     K  D+     F++   L    P NL      
Sbjct: 442 FQHPDDLKRCVRGIQTIERVVQSKAFSRYKYADV----SFEYLLNLTASTPVNLRPPRSG 501

Query: 497 ---SDNSSVQEYCKETVTTYWHYHGGCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTN 536
              S   S +E+C+ TVTT WHYHGGC+VG+VVD +Y+VIGI  LRV+D ST    PGTN
Sbjct: 502 PGASLPPSAEEFCQHTVTTIWHYHGGCVVGRVVDGDYKVIGIDRLRVIDMSTVGYCPGTN 561

[Close]

BLAST of CmaCh12G010120 vs. TAIR 10
Match: AT1G72970.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 401.7 bits (1031), Expect = 9.0e-112
Identity = 231/535 (43.18%), Postives = 311/535 (58.13%), Query Frame = 0


Query: 42  ENEEYDYIIIGGGTAGCPLAATLSSKFSVLLLERGSDP--NKYPSVLNEQGLLNVFVEED 101
           ++  YDYI+IGGGTAGCPLAATLS  FSVL+LERG  P  N   S L      ++ + + 
Sbjct: 59  QDSSYDYIVIGGGTAGCPLAATLSQNFSVLVLERGGVPFTNANVSFLRN---FHIGLADI 118

Query: 102 DGENPFQRFVSENGVEIIRGRVLGGGSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEW 161
              +  Q FVS +GV   R RVLGGGS +NAGFYSR    F + AG  WD +LV++ Y W
Sbjct: 119 SASSASQAFVSTDGVYNARARVLGGGSCINAGFYSRADAAFVKRAG--WDPKLVKESYPW 178

Query: 162 VEETVVSRPRLSAWQGAFRRALLEGGVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVEL 221
           VE  +V +P+L+ WQ A R +LLE GV P NGF   H+ GTK GG+IFD  G RH A EL
Sbjct: 179 VEREIVHQPKLTLWQKALRDSLLEVGVRPFNGFTYDHVSGTKIGGTIFDRFGRRHTAAEL 238

Query: 222 LNGVEPGNLKVAVRATVQKIIFSDLS----AIGVSYSDSEGKTHTAFI--RKKGEIILSA 281
           L    P  L+V + ATVQKI+F          GV + D +G  H A +  RK  E+ILS+
Sbjct: 239 LAYANPQKLRVLIYATVQKIVFDTSGTRPRVTGVIFKDEKGNQHQALLSNRKGSEVILSS 298

Query: 282 GAIGSPQLLLLSGVGPKSHLSSLKLPVVLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQ 341
           GAIGSPQ+L+LSG+GPK  L  LK+PVVL   HVG+ M+DNP  +  V    P+  S  Q
Sbjct: 299 GAIGSPQMLMLSGIGPKKELQRLKIPVVLENEHVGKGMADNPMNTILVPSKAPIEQSLIQ 358

Query: 342 VVGILDGNIHLQ--TFASPAPFFILPTFGLLPPQSTLINPSLA----------------- 401
            VGI    ++++  T    +P  I   +G++  ++ L +   A                 
Sbjct: 359 TVGITKMGVYVEASTGFGQSPESIHTHYGIMSNKNELFSTIPAKQRRPEATQAYITRNKY 418

Query: 402 ---------LLIGKFSDVLSKGSLHLNSSTDVKNNPIVRFNYYTNPNDLAQCVRGLRKVG 461
                     ++ K +  +S+G L L  +T+V +NP V FNY+ +P DL +CV  +R V 
Sbjct: 419 QLHEAFNGSFILEKLAYPISRGHLSL-VNTNVDDNPSVTFNYFKHPVDLQRCVEAIRLVS 478

Query: 462 DFLKTRTMEEIKTQDLEGNKGFQFLGPPL-----PENLSDNSSVQEYCKETVTTYWHYHG 521
             + +         D +       L         P+ L+D  S+ ++CK+TV T WHYHG
Sbjct: 479 KVVTSNRFLNYTQCDKQNVHKMLSLSVKANINLRPKQLNDTKSMAQFCKDTVVTIWHYHG 538

Query: 522 GCLVGKVVDRNYRVIGIKNLRVVDGSTFSVSPGTNPMATLMMLGRYVGLKMLQER 536
           GCLVGKVV  N +V+G+  LRV+DGSTF  SPGTNP AT+MM+GRY+G+K+L+ER
Sbjct: 539 GCLVGKVVSPNRKVLGVDRLRVIDGSTFDESPGTNPQATMMMMGRYMGVKILRER 587

[Close]

BLAST of CmaCh12G010120 vs. TAIR 10
Match: AT3G56060.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 389.0 bits (998), Expect = 6.0e-108
Identity = 237/565 (41.95%), Postives = 324/565 (57.35%), Query Frame = 0


Query: 8   ILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAATLSSK 67
           IL+++F        +  + +    + + DAT  P+   +DYIIIGGGTAGC LAATLS  
Sbjct: 12  ILVAVFIFH----DSCYSDEAGNYRFMKDATLAPKLSHFDYIIIGGGTAGCALAATLSQN 71

Query: 68  FSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLGGGSM 127
            +VL+LERG  P   P+  +     N  +      +  Q F+SE+GV   R RVLGGG++
Sbjct: 72  ATVLVLERGGSPYDDPAATDIGNFANTLLNITP-NSWSQLFISEDGVFNSRARVLGGGTV 131

Query: 128 VNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLEGGVG 187
           +NAGFYSR   +F   AG  W+ + VE  YEWVE+ VV  P ++ WQ AFR  LLE GV 
Sbjct: 132 INAGFYSRAEEDFVAEAG--WERDEVEAAYEWVEKKVVFEPPVNKWQSAFRDGLLEAGVT 191

Query: 188 PDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIFS----- 247
           P NGF   H+VGTK GG+IFD DG+RH A  LL    P  + V + A+V KI+F+     
Sbjct: 192 PYNGFTYEHIVGTKFGGTIFDRDGHRHTAANLLEYANPNMIVVYLHASVHKILFTIKGNQ 251

Query: 248 DLSAIGVSYSDSEGKTHTAFIRKK----GEIILSAGAIGSPQLLLLSGVGPKSHLSSLKL 307
              A GV + D+ G ++ A +  +     E+ILSAGAI SPQLL+LSGVGP +HL++ ++
Sbjct: 252 RPKAYGVIFLDANGVSYKAELATQDSTMSEVILSAGAIASPQLLMLSGVGPAAHLAAYRV 311

Query: 308 -PVVLHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGI------LDGNIHLQTFASPA 367
            PV++ QP VG+ M DNP     +  P P+  S  Q VGI      ++G   L    S  
Sbjct: 312 NPVIVDQPMVGQGMGDNPMNPVFIPSPEPVEVSLVQAVGITKFGSYIEGGSALSLSISLT 371

Query: 368 PFFILPTFGL-----LPPQS----------TL-INPSLALLIGKFSDVLSKGSLHLNSST 427
             F      L     LP QS          TL +     ++I K +  LS+G L L  +T
Sbjct: 372 RSFFDGVLNLLKKTKLPTQSISKFFKSLDLTLNVTTKAGVIIQKVNGPLSRGHLELR-NT 431

Query: 428 DVKNNPIVRFNYYTNPNDLAQCVRGLRKVGDFLKTRTMEEIKTQDLEGNKGFQFLGPPLP 487
           +  +NP V FNY+ +P DL +CV GL  +   + ++   + K   L   +G   L   LP
Sbjct: 432 NPDDNPSVTFNYFKDPEDLNKCVEGLSTIIKVIDSKGYSKYK-YPLASARGLLNLILALP 491

Query: 488 ENL-----SDNSSVQEYCKETVTTYWHYHGGCLVGKVVDRNYRVIGIKNLRVVDGSTFSV 536
            NL     +    +++YC +TV T +HYHGGC VGKVVD NY+V+G+  LR++DGSTF  
Sbjct: 492 TNLRPRHITSTFDLEQYCIDTVMTIYHYHGGCQVGKVVDNNYKVLGVDALRIIDGSTFLK 551

[Close]

BLAST of CmaCh12G010120 vs. TAIR 10
Match: AT5G51930.1 (Glucose-methanol-choline (GMC) oxidoreductase family protein )

HSP 1 Score: 388.7 bits (997), Expect = 7.8e-108
Identity = 233/556 (41.91%), Postives = 325/556 (58.45%), Query Frame = 0


Query: 5   LPLILISIFFLRVLSSHTIPNQDVSYMKLVHDATDIPENEEYDYIIIGGGTAGCPLAATL 64
           L L+ ISIFF  ++   T      ++MK   DAT  P+N  +DYIIIGGGTAGC LAATL
Sbjct: 41  LILVYISIFFFFLVKLET------TFMK---DATLAPKNASFDYIIIGGGTAGCALAATL 100

Query: 65  SSKFSVLLLERGSDPNKYPSVLNEQGLLNVFVEEDDGENPFQRFVSENGVEIIRGRVLGG 124
           S   SVL+LERG  P + P+  +    +N  +  +   +  Q F+SE+GV   R RVLGG
Sbjct: 101 SQNASVLVLERGGSPYENPTATDMGNSVNTLL-NNTPNSWSQLFISEDGVYNTRPRVLGG 160

Query: 125 GSMVNAGFYSRGHREFFESAGVDWDMELVEKGYEWVEETVVSRPRLSAWQGAFRRALLEG 184
           GS++N GFYSR   ++ E A  +W+ME VE  YEWVE+ +V  P++  WQ AF+  LLE 
Sbjct: 161 GSVINGGFYSRAGNDYVEEA--EWEMEEVEAAYEWVEKKLVFEPQVIEWQKAFKDGLLEA 220

Query: 185 GVGPDNGFDLRHLVGTKTGGSIFDEDGNRHGAVELLNGVEPGNLKVAVRATVQKIIFSDL 244
           G  PDNGF   H+ GTK GG+IFD  G+RH A  LL    P  + V + A+V K++F+  
Sbjct: 221 GESPDNGFTYDHIYGTKIGGTIFDRAGHRHTAANLLEYANPNRIVVYLHASVHKVLFT-T 280

Query: 245 SAIGVSYSDSEGKTHTAFIRKK--GEIILSAGAIGSPQLLLLSGVGPKSHLSSLKL-PVV 304
            A  V + D+ G  H A +  K   E+ILSAGA+GSPQLL+LSGVGP  HL +  + P+V
Sbjct: 281 EAYEVLFEDANGVFHKANLANKATNEVILSAGALGSPQLLMLSGVGPAVHLEAHGVNPLV 340

Query: 305 LHQPHVGEFMSDNPRFSAAVVLPFPLVASSAQVVGILDGNIHLQTFASPAPFFILP---- 364
           L QP VG+ M+DNP    A+  P P+  S  Q VGI   + +++  +  +  F +     
Sbjct: 341 LDQPMVGQGMADNPMNFVAIPSPQPVELSLIQAVGITKFDSYIEGLSGLSLSFDITRRFF 400

Query: 365 --TFGLLPPQS-----TLINPSLALLIGKFSDVL-----------------SKGSLHLNS 424
                LL   S      ++  S+A+L+  F   L                 SKG + L  
Sbjct: 401 DGVLNLLNETSHTTSRKILTQSIAVLLKSFDVKLEVRMNGGLIFQKVDGPASKGHMKLR- 460

Query: 425 STDVKNNPIVRFNYYTNPNDLAQCVRGLRKVGDFLKTRTMEEIKTQDLEGNKGFQ-FLGP 484
           +T+ ++NP V FNYY  P DL +CV+GL  +   + ++   + K   +   +     L  
Sbjct: 461 NTNPRDNPSVTFNYYQEPEDLNKCVKGLNTIIRMINSKAFSKYKYPGVTARELLNLMLAL 520

Query: 485 PL---PENLSDNSSVQEYCKETVTTYWHYHGGCLVGKVVDRNYRVIGIKNLRVVDGSTFS 526
           P+   P +++   +++++C +TVT+ WHYHGGC VGKVVD+NY+V+GI  LRV+DGSTF 
Sbjct: 521 PINLRPRHVTSAFNLKQFCIDTVTSVWHYHGGCQVGKVVDKNYKVLGIDGLRVIDGSTFL 580

The following BLAST results are available for this feature:

BLAST of CmaCh12G010120 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P52706	1.0e-152	50.74	(R)-mandelonitrile lyase 1 OS=Prunus serotina OX=23207 GN=MDL1 PE=1 SV=1	[more]
P52707	2.5e-151	49.91	(R)-mandelonitrile lyase 3 OS=Prunus serotina OX=23207 GN=MDL3 PE=2 SV=1	[more]
Q945K2	7.3e-151	50.00	(R)-mandelonitrile lyase 2 OS=Prunus dulcis OX=3755 GN=MDL2 PE=1 SV=1	[more]
Q9SSM2	2.0e-148	50.38	(R)-mandelonitrile lyase-like OS=Arabidopsis thaliana OX=3702 GN=At1g73050 PE=2 ...	[more]
O50048	2.6e-148	50.00	(R)-mandelonitrile lyase 2 OS=Prunus serotina OX=23207 GN=MDL2 PE=2 SV=1	[more]

BLAST of CmaCh12G010120 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HRM5	2.0e-310	100.00	(R)-mandelonitrile lyase 1-like OS=Cucurbita maxima OX=3661 GN=LOC111465498 PE=3...	[more]
A0A6J1FC90	7.0e-298	95.72	(R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111444087 PE...	[more]
A0A6J1G4V5	1.5e-271	86.41	(R)-mandelonitrile lyase 1-like OS=Cucurbita moschata OX=3662 GN=LOC111450816 PE...	[more]
A0A5D3CL00	4.2e-250	81.09	(R)-mandelonitrile lyase 1-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_...	[more]
A0A1S3BSP4	4.2e-250	81.09	(R)-mandelonitrile lyase 1-like OS=Cucumis melo OX=3656 GN=LOC103493073 PE=3 SV=...	[more]

BLAST of CmaCh12G010120 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022965669.1	4.1e-310	100.00	(R)-mandelonitrile lyase 1-like [Cucurbita maxima]	[more]
XP_022937802.1	1.5e-297	95.72	(R)-mandelonitrile lyase 1-like [Cucurbita moschata]	[more]
XP_023546454.1	2.3e-274	87.15	(R)-mandelonitrile lyase 1-like [Cucurbita pepo subsp. pepo]	[more]
XP_022946877.1	3.0e-271	86.41	(R)-mandelonitrile lyase 1-like [Cucurbita moschata]	[more]
KAG7030241.1	5.8e-262	87.77	(R)-mandelonitrile lyase 1, partial [Cucurbita argyrosperma subsp. argyrosperma]	[more]

BLAST of CmaCh12G010120 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G73050.1	1.4e-149	50.38	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G12570.1	6.2e-113	42.32	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT1G72970.1	9.0e-112	43.18	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT3G56060.1	6.0e-108	41.95	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]
AT5G51930.1	7.8e-108	41.91	Glucose-methanol-choline (GMC) oxidoreductase family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR036188	FAD/NAD(P)-binding domain superfamily	GENE3D	3.50.50.60		coord: 46..524 e-value: 4.2E-168 score: 562.3
IPR036188	FAD/NAD(P)-binding domain superfamily	SUPERFAMILY	51905	FAD/NAD(P)-binding domain	coord: 27..534
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PFAM	PF00732	GMC_oxred_N	coord: 46..314 e-value: 1.0E-32 score: 113.7
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00623	GMC_OXRED_1	coord: 119..142
IPR000172	Glucose-methanol-choline oxidoreductase, N-terminal	PROSITE	PS00624	GMC_OXRED_2	coord: 274..288
None	No IPR available	GENE3D	3.30.410.40		coord: 166..474 e-value: 4.2E-168 score: 562.3
None	No IPR available	PIRSR	PIRSR000137-2	PIRSR000137-2	coord: 384..535 e-value: 2.0E-39 score: 133.6
None	No IPR available	PANTHER	PTHR45968	OSJNBA0019K04.7 PROTEIN	coord: 7..536
None	No IPR available	PANTHER	PTHR45968:SF23	(R)-MANDELONITRILE LYASE 4-RELATED	coord: 7..536
None	No IPR available	SUPERFAMILY	54373	FAD-linked reductases, C-terminal domain	coord: 353..481
IPR012132	Glucose-methanol-choline oxidoreductase	PIRSF	PIRSF000137	Alcohol_oxidase	coord: 26..346 e-value: 8.7E-58 score: 194.4 coord: 343..537 e-value: 1.1E-30 score: 104.9
IPR007867	Glucose-methanol-choline oxidoreductase, C-terminal	PFAM	PF05199	GMC_oxred_C	coord: 385..525 e-value: 9.4E-29 score: 100.8

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G010120.1	CmaCh12G010120.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
molecular_function	GO:0050660	flavin adenine dinucleotide binding
molecular_function	GO:0016829	lyase activity
molecular_function	GO:0016614	oxidoreductase activity, acting on CH-OH group of donors