CmaCh12G009350 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh12G009350 vs. ExPASy Swiss-Prot
Match: Q9FKG6 (U-box domain-containing protein 52 OS=Arabidopsis thaliana OX=3702 GN=PUB52 PE=2 SV=1)

HSP 1 Score: 407.5 bits (1046), Expect = 3.5e-112
Identity = 288/819 (35.16%), Postives = 438/819 (53.48%), Query Frame = 0

Query: 12  SVAVAIDKDKFSQQALKWTIERLLSRGQV-LTLLHVKHKPSHSTTNVG---ACSDLDDAV 71
           SVAVAI+  K S+  + W +E+ +  G     LL+V+   S+  T +G   A S+L + V
Sbjct: 23  SVAVAINGKKKSKYVVFWALEKFIPEGFTDFKLLYVRPPVSYIPTPMGIAVAVSELREDV 82

Query: 72  --AHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDVLVLGA 131
             A+KQ++D  A++   P++    R  +    + L+  + + A+        +  LV+G 
Sbjct: 83  VSAYKQELDWSANEMLRPYKKMFERRKVQVEVLLLDSLEPAAAIAEEIAGTGVTKLVIGM 142

Query: 132 PSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPA-----KPISTS 191
             R G   R K D+ S+++   P FCTVY I +KGK++S+R + S A       +  STS
Sbjct: 143 SLR-GFFSR-KIDMSSLIATAVPRFCTVYVI-SKGKLASVRPSESDASGSIRFERSSSTS 202

Query: 192 HSQPQPASRPQLLHQLTNASKA----SVEHPTILRSQHSKHLETQDSLASNPPSRAIHED 251
            S   P   P+    L+  S+A    S   P +  S  S  +   D+ +S      +   
Sbjct: 203 GSTDSPRLPPEYQDFLSAVSEAQSRVSPFSPALKHSMGSNAVAQMDTSSSGTDQEEVSTG 262

Query: 252 FEIKSPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNG 311
             ++   +      + GK       + D SF +S     +  NS +W S  RD       
Sbjct: 263 RGMEIVHS-----GIEGK------KNKDESFSASFPMGTEAYNSMSWTSKWRD------- 322

Query: 312 SGSELETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQ 371
                E  S  S++   ++ M     +  N    YS   SH  S  S+   S  S +  Q
Sbjct: 323 HEDRREMRSSSSSNNHDLVNMDWGAVVPEN----YS-WVSHTASHMSDGLLSVHSITDNQ 382

Query: 372 EDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAAL 431
            ++  E+ +L+ ELK   +MY+ A  E + A +K +EL++ + EE  +L E +  E  A 
Sbjct: 383 VNLNFEIEKLRAELKHVQEMYAMAQTETVGASKKLTELNQRRFEESEKLVELKEKEEVAK 442

Query: 432 AIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYR 491
             A KEK + + A++ AE  +   + EA  R   E  A  +A E  K         + Y+
Sbjct: 443 DTASKEKQRYEEAMKEAEKVKELMMKEALHRREAEFKAERDAREKDKLQASLVSPGVQYQ 502

Query: 492 RYSIQEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEV 551
            Y+ +EI  AT+ F+ +LKIG G YG VY+  L HT  A+KVL     Q   QF +E+E+
Sbjct: 503 HYTWEEIAAATSDFAENLKIGIGAYGSVYKCNLHHTTGAVKVLHAGETQLSKQFDQELEI 562

Query: 552 LSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATG 611
           LS IRHP++VLLLGACPE GCLVYE+M NGSL+D L    + P +PW  RFRIA E+A+ 
Sbjct: 563 LSKIRHPHLVLLLGACPERGCLVYEYMDNGSLDDRLMLVNDTPPIPWFERFRIALEVASA 622

Query: 612 LLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGT 671
           L+FLH+++P PI+HRDLKPGNILLD N+VSK+ DVGL+ +V     +S T ++ T+  GT
Sbjct: 623 LVFLHKSKPRPIIHRDLKPGNILLDHNFVSKLGDVGLSTMVNQDDVSSRTIFKQTSPVGT 682

Query: 672 FFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAI-EKGKFAEMLDPD 731
             YIDPEYQ+TG++  KSDVYSLGV++LQ+ITAKP I + H VE AI +  +F  +LD  
Sbjct: 683 LCYIDPEYQRTGIISPKSDVYSLGVVILQLITAKPAIAITHMVEEAIGDDAEFMAILDKK 742

Query: 732 VKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESM--------GIQ 791
              WP+ +  + A L + C EMR+RDRPDL   ++P L RLR +A+++         G  
Sbjct: 743 AGSWPISDTRELAALGLCCTEMRRRDRPDLKDQIIPALERLRKVADKAQNLLSRTPSGPP 802

Query: 792 PYSIC-------DSPCMSQASFSQEQFSLHQGLDESESS 800
            + IC       + PC++   ++ ++ ++ + L + ++S
Sbjct: 803 SHFICPLLKGVMNEPCVAADGYTYDREAIEEWLRQKDTS 815

BLAST of CmaCh12G009350 vs. ExPASy Swiss-Prot
Match: Q9SW11 (U-box domain-containing protein 35 OS=Arabidopsis thaliana OX=3702 GN=PUB35 PE=2 SV=2)

HSP 1 Score: 393.7 bits (1010), Expect = 5.2e-108
Identity = 276/812 (33.99%), Postives = 435/812 (53.57%), Query Frame = 0

Query: 12  SVAVAIDKDKFSQQALKWTIERLLSRGQV-LTLLHVKHKPSHSTTNVGACSDL----DDA 71
           +V VA+     S+  + W IE+  + G V   LLH+    +   T +G    +    DD 
Sbjct: 21  TVVVALSGSSKSKYVVTWAIEKFATEGNVGFKLLHIHPMITSVPTPMGNAIPISEVRDDV 80

Query: 72  V-AHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDVLVLGA 131
           V A++Q++  Q+ +   P+     R  +    + +E  +++ A+     +++ID +V+G 
Sbjct: 81  VTAYRQEILWQSEEMLKPYTKLFVRRKVAVEVLVIESDNVAAAIAEEVTRDSIDRIVIGG 140

Query: 132 PSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTSHSQPQ 191
            SR+   +  K+DI S++S   P+FCTVY + +KGK+S +R + S   A  I    S+  
Sbjct: 141 SSRSFFSR--KADICSVISALMPNFCTVYVV-SKGKLSCVRPSDSDGNA-TIREDGSERT 200

Query: 192 PASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIKSPFNR 251
            +S        + +S  + +   ++ S H    ++Q    S P  R  H           
Sbjct: 201 NSS--------SGSSGPTSDSSDVMSSAH----DSQSRPLSLPVRRMQH----------F 260

Query: 252 PGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLS-NGSGSELETS 311
           P          +TS+  SD +   S      R  S    SST  + R +      E    
Sbjct: 261 PAIAGQASVPMETSSVGSDETRCMSLDAEEARDVSSINRSSTDTTSRWTPRRRDYEERKE 320

Query: 312 SPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVEAEMR 371
           +  S+S +   G  G R   S      ++  + + +S      S  S +  Q ++  E+ 
Sbjct: 321 AMSSSSSNREYGNFGTRFSWSGMGVDTTHSRASQQASNMSDALSEQSYTDNQVNLNFEVE 380

Query: 372 RLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAEKEKL 431
           +L+ EL+   +MY+ A  E  +A +K  EL++ ++EE  +LEE ++ E  A  +AEKEK 
Sbjct: 381 KLRAELRHVQEMYAVAQTETFDASRKLGELNQRRLEEAIKLEELKLKEYEARELAEKEKQ 440

Query: 432 KCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSIQEIE 491
             + A + AE  +  A  E  +R   E+ +  + +E +K     G   + Y+ ++ +EI 
Sbjct: 441 NFEKARRDAESMRERAEREIAQRREAERKSARDTKEKEKLEGTLGSPQLQYQHFAWEEIM 500

Query: 492 TATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPN 551
            AT+ FS  LKIG G YG VY+  L HT   +KVL+    Q   QFQ+E+E+LS IRHP+
Sbjct: 501 AATSSFSEELKIGMGAYGAVYKCNLHHTTAVVKVLQSAENQLSKQFQQELEILSKIRHPH 560

Query: 552 MVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTR 611
           +VLLLGACPE G LVYE+M NGSLED LF+    P LPW  RFRIA E+A  L+FLH+++
Sbjct: 561 LVLLLGACPEQGALVYEYMENGSLEDRLFQVNNSPPLPWFERFRIAWEVAAALVFLHKSK 620

Query: 612 PEPIVHRDLKPGNILLDRNYVSKISDVGLARLVP-PSVANSVTQYRMTATAGTFFYIDPE 671
           P+PI+HRDLKP NILLD N+VSK+ DVGL+ +V    ++   T Y+ T+  GT  YIDPE
Sbjct: 621 PKPIIHRDLKPANILLDHNFVSKVGDVGLSTMVQVDPLSTKFTIYKQTSPVGTLCYIDPE 680

Query: 672 YQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKG-KFAEMLDPDVKDWPVE 731
           YQ+TG +  KSD+YS G++LLQ++TAKP I L H VE A++   +F ++LD    +WP+E
Sbjct: 681 YQRTGRISSKSDIYSFGMILLQLLTAKPAIALTHFVESAMDSNDEFLKILDQKAGNWPIE 740

Query: 732 EALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEES------MGIQP--YSIC-- 791
           E  + A L++ C E+R +DRPDL   +LP L  L+ +AE++      +  QP  + IC  
Sbjct: 741 ETRELAALALCCTELRGKDRPDLKDQILPALENLKKVAEKARNSFSGVSTQPPTHFICPL 800

Query: 792 -----DSPCMSQASFSQEQFSLHQGLDESESS 800
                + PC++   ++ ++ ++ + L E  +S
Sbjct: 801 LKDVMNEPCVAADGYTYDRHAIEEWLKEHNTS 806

BLAST of CmaCh12G009350 vs. ExPASy Swiss-Prot
Match: Q9FKG5 (U-box domain-containing protein 51 OS=Arabidopsis thaliana OX=3702 GN=PUB51 PE=2 SV=2)

HSP 1 Score: 387.5 bits (994), Expect = 3.7e-106
Identity = 285/826 (34.50%), Postives = 429/826 (51.94%), Query Frame = 0

Query: 13  VAVAI-DKDKFSQQALKWTIERLLSRGQVL-TLLHVKHKPSHSTTNVGACSDLDDAVAHK 72
           VAVAI   +  ++  ++W ++   S+  V+  LLHV+ + S+S +      DL  +V +K
Sbjct: 8   VAVAIKGNNSKTKGVVRWALQEFASQEHVVFKLLHVQPRDSNSVSTTR--KDLTTSV-YK 67

Query: 73  QQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDVLVLGAPSRTG 132
           + VD +  +  LP R       +  + + LE  DI+ A+      + I  LV+GA S   
Sbjct: 68  KDVDRKTREMLLPSRDMFVHREVQLDIMVLESDDIADAISKAVQDHGISELVIGASSSII 127

Query: 133 IVKRFK-SDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTSHSQPQPASR 192
              + K S++ S ++   P FC+V+ I +KGK                            
Sbjct: 128 FSWKLKRSNLSSRIADATPRFCSVHVI-SKGK---------------------------- 187

Query: 193 PQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIKSPFNRPGSR 252
                 L N  K+ ++  T +    S+   + DS +    S + H+       F R   +
Sbjct: 188 ------LLNVRKSDMDTETSIADDRSESRFSSDSHSGTVSSTSSHQFSSTPLLFQR--IQ 247

Query: 253 ALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTR------DSYRLSNGSGSELET 312
           AL    Q   T        + G+Q+    N P  +   R      D  +L N  G    T
Sbjct: 248 ALTTVNQKVGT--------NIGKQN----NEPHHHHHNRAGSLDVDESKLLNQKGF-YRT 307

Query: 313 SSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVEAEM 372
           SS G       +G GG      ++ SS+ +    E SS+S      +S S   +D E E 
Sbjct: 308 SSSG-------IGYGG------SDISSWRSSQMEEASSSSTYSDPTSSSSQIHKDFELE- 367

Query: 373 RRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAEKEK 432
            +LK+EL+    MY+ A  E ++A +K  +L++ + EE  RL+   + E  A  + E E+
Sbjct: 368 -KLKIELRHIKGMYAVAQSEVIDASKKMQDLNQRRSEEATRLKNLTIREEEADEVVEMER 427

Query: 433 LKCKAALQAAEVAQRSAVHEARKRANVEKLAV---MEAEETKKALEGFGHGDIGYRRYSI 492
            + + A   AE+ +     E  +R   E  A     E +  + ALEG       Y ++  
Sbjct: 428 ERQEDAENEAELVRECIERETEERLEAEARAEEVRKEKQRLEDALEGGPLQRQQYMKFEW 487

Query: 493 QEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCI 552
           +EI  AT+ FS  LKIG GGYG VYR  L HT VA+KVL  D +    QF +E+E+LS I
Sbjct: 488 EEIVEATSSFSDELKIGVGGYGSVYRCNLHHTTVAVKVLHSDKSSLTKQFHQELEILSKI 547

Query: 553 RHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRK------PEDPVLPWQLRFRIAEEIA 612
           RHP+++LLLGACPE G LVYE+M NGSLE+ L ++      P+ P L W  RFRIA EIA
Sbjct: 548 RHPHLLLLLGACPERGSLVYEYMHNGSLEERLMKRRPNVDTPQPPPLRWFERFRIAWEIA 607

Query: 613 TGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATA 672
           + L FLH   P PIVHRDLKP NILLDRN VSKI DVGL+++V    +++ T +  T   
Sbjct: 608 SALYFLHTNEPRPIVHRDLKPANILLDRNNVSKIGDVGLSKMVNLDPSHASTVFNETGPV 667

Query: 673 GTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAI--EKGKFAEML 732
           GTFFYIDPEYQ+TG++  +SD+Y+ G++LLQ++TA+  +GLAH++E+A+  + GKF E+L
Sbjct: 668 GTFFYIDPEYQRTGVVTPESDIYAFGIILLQLVTARSAMGLAHSIEKALRDQTGKFTEIL 727

Query: 733 DPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPYSIC 792
           D    DWPV+EA +   + ++CAEMRKRDRPDLGK +LP L RL+ +A  +  +   ++ 
Sbjct: 728 DKTAGDWPVKEAKEMVMIGLRCAEMRKRDRPDLGKEILPVLERLKEVASIARNMFADNLI 765

Query: 793 D-------------------SPCMSQASFSQEQFSLHQGLDESESS 800
           D                   +PC++   ++ E+ ++ + L ++  S
Sbjct: 788 DHHHNAPTHFYCPITKDVMENPCVASDGYTYEKRAIKEWLQKNHKS 765

BLAST of CmaCh12G009350 vs. ExPASy Swiss-Prot
Match: Q8S8S7 (U-box domain-containing protein 34 OS=Arabidopsis thaliana OX=3702 GN=PUB34 PE=3 SV=1)

HSP 1 Score: 361.7 bits (927), Expect = 2.2e-98
Identity = 211/471 (44.80%), Postives = 286/471 (60.72%), Query Frame = 0

Query: 287 TRDSYRLSNGSGSELETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYS-HEMSSASES 346
           T  S + S+ +  E+     G  S  P L         +   S+  N  S H  S  S  
Sbjct: 243 TPQSSKASSATTPEIFRRRRG--SDIPQLNYSDFDKTCTKPQSNVENIVSEHRDSDRSPP 302

Query: 347 ETSWASQSHAQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRL 406
           ETS  S+   + ++E E+ RLK EL+ T+  Y  AC+E  + + K   L    + E  R+
Sbjct: 303 ETSRKSK---KVEIEEEVERLKNELQSTVFKYKQACEELFSTQNKVKMLSTEYLNESKRV 362

Query: 407 EEARMAEGAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKAL 466
             A   E      A  EK +   A++  E A+     E  +R   E  A+    E KK +
Sbjct: 363 NNAVEKEELQRNTAALEKERYMKAVKEVETAKALLAREFCQRQIAEVNALRTYLEKKKVI 422

Query: 467 EGFGHGDIGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQ 526
           +     D  YR+Y+I+EI TAT GFS    IGEGGYG VY+  LD T  A+KV+R D  +
Sbjct: 423 DQLLGTDHRYRKYTIEEIVTATEGFSPEKVIGEGGYGKVYQCSLDSTPAAVKVVRLDTPE 482

Query: 527 GRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQL 586
            + +F +EVEVLS +RHP++VLLLGACPENGCLVYE++ NGSLE+ +F +   P LPW +
Sbjct: 483 KKQEFLKEVEVLSQLRHPHVVLLLGACPENGCLVYEYLENGSLEEYIFHRKNKPPLPWFI 542

Query: 587 RFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSV 646
           RFR+  E+A GL FLH ++PEPIVHRDLKPGNILL+RNYVSKI+DVGLA+LV     ++V
Sbjct: 543 RFRVIFEVACGLAFLHSSKPEPIVHRDLKPGNILLNRNYVSKIADVGLAKLVTDVAPDNV 602

Query: 647 TQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEK 706
           T YR +  AGT  YIDPEY +TG +  KSD+Y+ G+++LQ++TA+ P G+   VE A++K
Sbjct: 603 TMYRNSVLAGTLHYIDPEYHRTGTIRPKSDLYAFGIIILQLLTARNPSGIVPAVENAVKK 662

Query: 707 GKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRL 757
           G   EMLD  V DWP+ E  + A++ ++CAE R RDRPDL   V+P L RL
Sbjct: 663 GTLTEMLDKSVTDWPLAETEELARIGLKCAEFRCRDRPDLKSEVIPVLKRL 708

BLAST of CmaCh12G009350 vs. ExPASy Swiss-Prot
Match: Q9LU47 (Putative U-box domain-containing protein 53 OS=Arabidopsis thaliana OX=3702 GN=PUB53 PE=3 SV=1)

HSP 1 Score: 331.3 bits (848), Expect = 3.2e-89
Identity = 250/835 (29.94%), Postives = 404/835 (48.38%), Query Frame = 0

Query: 12  SVAVAIDKDKFSQQALKWTIERLLS-RGQVLTLLHVKHK----PSHSTTNVGACSDLDD- 71
           +VA+AI     S+  +KW + +  S +     L+H+  K    P+ S   V    +L++ 
Sbjct: 33  TVALAISGSIKSKNVIKWALNKFGSDKNVTFKLIHIHPKITTLPTASGNIVSISEELEEV 92

Query: 72  AVAHKQQVDNQAHQFFL-PFRCFCTRSNIPCNEV--------------TLEEGDISKALI 131
           A A++Q+V  +  +  L PF+  C R  +  +E                LE   ++ A+ 
Sbjct: 93  AAAYRQKVMQETKETLLKPFKKMCERKKLKIDETRFESSLTKVAVELQVLESNSVAVAIT 152

Query: 132 NYANKNAIDVLVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATS 191
              N++ I  L++G  S+    + +  DI + +S    + CTVY + N G V  + + TS
Sbjct: 153 KEVNQHLISNLIIGRSSQAASSRNY--DITASISASVSNLCTVYVVSN-GGVHILAKDTS 212

Query: 192 PAPAKPISTSHSQPQPASRPQLLHQLTN--ASKASVEHPTILRSQHSKHLETQDSLASNP 251
                  S      + +S         +   S A   +P  L ++  ++L T        
Sbjct: 213 DTERNDTSIESGFERTSSSCSSGSGANSDVMSNALKSNPHTLSNKRMQNLPT-------- 272

Query: 252 PSRAIHEDFEIKSPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTR 311
             R +    E  S            +  +T    SD +  +S R S +   S +WN   R
Sbjct: 273 IVRGVSVPMETSST-----------ESDETKKRSSDAAEEASKRSSPETSRSVSWNPQFR 332

Query: 312 DSYRLSNGSGSELETSSPGSASPSPMLGMGGMRSIDSNNNSSYSN---PYSHEMSSASES 371
           D     +   S                          ++N  Y N   P  H  +     
Sbjct: 333 DFDERKDAMSS-------------------------MSSNFEYGNVVTPLGHYFTD---- 392

Query: 372 ETSWASQSHAQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRL 431
                      +D   E+ +L+ EL+   +MY+ A  E L+A +K +EL         + 
Sbjct: 393 ----------NQDTLNEISKLRAELRHAHEMYAVAQVETLDASRKLNEL---------KF 452

Query: 432 EEARMAEGAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKAL 491
           EE  + E     IA+KE  K +         +R    EA +R   E  A  EA+E +K  
Sbjct: 453 EELTLLEHETKGIAKKETEKFE--------QKRREEREAAQRREAEMKATHEAKEKEKLE 512

Query: 492 E-GFGHGDIGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAA 551
           E       + Y+ ++ +EI  AT+ FS  LKIG G YG VY+  L HT  A+KVL    +
Sbjct: 513 ESSLVAPKLQYQEFTWEEIINATSSFSEDLKIGMGAYGDVYKCNLHHTIAAVKVLHSAES 572

Query: 552 QGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQ 611
               QF +E+E+LS IRHP++VLLLGACP++G LVYE+M NGSLED LF+  +   +PW 
Sbjct: 573 SLSKQFDQELEILSKIRHPHLVLLLGACPDHGALVYEYMENGSLEDRLFQVNDSQPIPWF 632

Query: 612 LRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPS--VA 671
           +R RIA E+A+ L+FLH+++P PI+HRDLKP NILL+ N+VSK+ DVGL+ ++  +  ++
Sbjct: 633 VRLRIAWEVASALVFLHKSKPTPIIHRDLKPANILLNHNFVSKVGDVGLSTMIQAADPLS 692

Query: 672 NSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERA 731
              T Y+ T+  GT  YIDPEYQ+TG +  KSDVY+ G+++LQ++T +  + L + VE A
Sbjct: 693 TKFTMYKQTSPVGTLCYIDPEYQRTGRISPKSDVYAFGMIILQLLTGQQAMALTYTVETA 752

Query: 732 IEKG---KFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAM 791
           +E     +  ++LD    +WP+EE  + A L++QC E+R +DRPDL   +LP L  L+ +
Sbjct: 753 MENNNDDELIQILDEKAGNWPIEETRQLAALALQCTELRSKDRPDLEDQILPVLESLKKV 789

Query: 792 AEESMG------IQPYS---------ICDSPCMSQASFSQEQFSLHQGLDESESS 800
           A+++         QP S         +   PC++   ++ ++ ++ + ++   +S
Sbjct: 813 ADKARNSLSAAPSQPPSHFFCPLLKDVMKEPCIAADGYTYDRRAIEEWMENHRTS 789

BLAST of CmaCh12G009350 vs. ExPASy TrEMBL
Match: A0A6J1HM11 (U-box domain-containing protein 52-like OS=Cucurbita maxima OX=3661 GN=LOC111465786 PE=4 SV=1)

HSP 1 Score: 1529.2 bits (3958), Expect = 0.0e+00
Identity = 784/784 (100.00%), Postives = 784/784 (100.00%), Query Frame = 0

Query: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC 60
           MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC
Sbjct: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC 60

Query: 61  SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV 120
           SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV
Sbjct: 61  SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV 120

Query: 121 LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS 180
           LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS
Sbjct: 121 LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS 180

Query: 181 HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK 240
           HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK
Sbjct: 181 HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK 240

Query: 241 SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE 300
           SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE
Sbjct: 241 SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE 300

Query: 301 LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360
           LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE
Sbjct: 301 LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360

Query: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAE 420
           AEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAE
Sbjct: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAE 420

Query: 421 KEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSI 480
           KEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSI
Sbjct: 421 KEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSI 480

Query: 481 QEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCI 540
           QEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCI
Sbjct: 481 QEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCI 540

Query: 541 RHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFL 600
           RHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFL
Sbjct: 541 RHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFL 600

Query: 601 HQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYI 660
           HQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYI
Sbjct: 601 HQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYI 660

Query: 661 DPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWP 720
           DPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWP
Sbjct: 661 DPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWP 720

Query: 721 VEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPYSICDSPCMSQA 780
           VEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPYSICDSPCMSQA
Sbjct: 721 VEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPYSICDSPCMSQA 780

Query: 781 SFSQ 785
           SFSQ
Sbjct: 781 SFSQ 784

BLAST of CmaCh12G009350 vs. ExPASy TrEMBL
Match: A0A6J1FHP0 (U-box domain-containing protein 52-like OS=Cucurbita moschata OX=3662 GN=LOC111444053 PE=4 SV=1)

HSP 1 Score: 1513.4 bits (3917), Expect = 0.0e+00
Identity = 787/847 (92.92%), Postives = 798/847 (94.21%), Query Frame = 0

Query: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC 60
           MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVG+ 
Sbjct: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGSY 60

Query: 61  SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV 120
           SD DDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINY NKN IDV
Sbjct: 61  SDSDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYVNKNVIDV 120

Query: 121 LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS 180
           LVLGAPSRTG+VKRFKSDIPSIVSKGAPDFCTVY IGNKGKVSSMRQATSPAPAKPISTS
Sbjct: 121 LVLGAPSRTGLVKRFKSDIPSIVSKGAPDFCTVYIIGNKGKVSSMRQATSPAPAKPISTS 180

Query: 181 HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK 240
           H+QPQPA+RPQLLHQLTNAS+ SVEHPTILRSQ S+HLETQDSLASNPP RAIHEDFEIK
Sbjct: 181 HNQPQPATRPQLLHQLTNASEVSVEHPTILRSQPSRHLETQDSLASNPPFRAIHEDFEIK 240

Query: 241 SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE 300
           SPFNRPGSRA MGKGQDTSTADSDISF+SSGRQSLDRGNSPAWNS T DSYRLSNGS   
Sbjct: 241 SPFNRPGSRASMGKGQDTSTADSDISFVSSGRQSLDRGNSPAWNSGTGDSYRLSNGS--- 300

Query: 301 LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360
            ETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE
Sbjct: 301 -ETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360

Query: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQK-----------------------------A 420
           AEMRRLKLELKQTMDMYSTACKEALNAKQK                             A
Sbjct: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQKVSIISKISNLFSMIYNHDSTSNSNRLIWQA 420

Query: 421 SELHRWKMEEEHRLEEARMAEGAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVE 480
           SELHRWKMEEEHRLEEARMAE AALAIAE+EKLKCKAALQAAE+AQRSAV EARKRANVE
Sbjct: 421 SELHRWKMEEEHRLEEARMAEEAALAIAEQEKLKCKAALQAAELAQRSAVQEARKRANVE 480

Query: 481 KLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDH 540
           KLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSA+LKIGEGGYGPVYRGELDH
Sbjct: 481 KLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSAALKIGEGGYGPVYRGELDH 540

Query: 541 TQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDC 600
           TQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDC
Sbjct: 541 TQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDC 600

Query: 601 LFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDV 660
           LFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDV
Sbjct: 601 LFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDV 660

Query: 661 GLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKP 720
           GLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKP
Sbjct: 661 GLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKP 720

Query: 721 PIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLP 780
           PIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLP
Sbjct: 721 PIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLP 780

Query: 781 ELSRLRAMAEESMGIQPYSICDSPCMSQASFSQEQFSLHQGLDESESSYCHSGSSTSSYS 819
           ELSRLRAMAEESMG QP+SICDSPCMSQASFSQEQFSLHQGLDE ESSYC SGSSTSSYS
Sbjct: 781 ELSRLRAMAEESMGFQPFSICDSPCMSQASFSQEQFSLHQGLDECESSYCQSGSSTSSYS 840

BLAST of CmaCh12G009350 vs. ExPASy TrEMBL
Match: A0A6J1EUU5 (U-box domain-containing protein 35-like OS=Cucurbita moschata OX=3662 GN=LOC111436140 PE=4 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.9e-294
Identity = 578/826 (69.98%), Postives = 657/826 (79.54%), Query Frame = 0

Query: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPS---HSTTNV 60
           MARGGA KK+  + VAIDKDK SQQALKWTI+RLLSRGQV+TLLHVKHK S   ++TTN+
Sbjct: 1   MARGGAQKKE--MVVAIDKDKGSQQALKWTIDRLLSRGQVVTLLHVKHKSSSTANATTNL 60

Query: 61  GACSDLDDAVAHK-QQVDNQAH-QFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANK 120
            A  D  +   +K   +DN  + QFFLPFRC CT SNI CNEV LE  DI K LINY +K
Sbjct: 61  KADFDHGETALYKHHHIDNHINDQFFLPFRCICTSSNITCNEVMLEGSDIPKTLINYVSK 120

Query: 121 NAIDVLVLGAPSRTG--IVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAP 180
           N +D+LV+GAP+R+   I KRFKSD+PS VSKGAPDFCTVYTIG++GKV S+RQ T P P
Sbjct: 121 NVVDILVMGAPTRSAAQIYKRFKSDVPSSVSKGAPDFCTVYTIGHRGKV-SVRQPTVPVP 180

Query: 181 AKPISTSHSQPQPAS-RPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRA 240
           A+PI+   +Q   AS RPQLLHQ TN S+   E P  L S+ S+ LETQ S+       A
Sbjct: 181 ARPIAPPRNQSLRASPRPQLLHQRTNVSQLLAE-PNPLWSKSSRCLETQSSMR---VPHA 240

Query: 241 IHEDFEIKSPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSST-RDSY 300
           + +  + KSPF R GSRA + K  D ST DSD SF+S GR+S+DRG S A + +T RDSY
Sbjct: 241 LQQYLDFKSPFTRQGSRASVSKRVDPSTVDSDNSFVSCGRRSVDRGFSGALSYATCRDSY 300

Query: 301 RLSNGSGSELETSSPGSASPSPMLGM--GGMRSIDSNNNSSYSNPYSHEMSSASESET-S 360
           RLSN S SE  +  P   SPSP L +  G  +SI +NN SSYS   S   S + ES+T +
Sbjct: 301 RLSNVSESETSSPGPPPPSPSPTLEIESGMNKSIHNNNFSSYSQDLS---SCSLESDTFT 360

Query: 361 WASQSHAQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEA 420
             S SHAQ+DVEAEMRRLKLELKQTMDMYSTACKEALNA +KA+ELHRWK+EEE R EEA
Sbjct: 361 RPSHSHAQDDVEAEMRRLKLELKQTMDMYSTACKEALNANRKATELHRWKVEEEQRFEEA 420

Query: 421 RMAEGAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGF 480
           RMAE AALA+AE+EK+KC+AA++AAE AQ+ A  EARKR N E++A MEAEETKKAL GF
Sbjct: 421 RMAEEAALAMAEREKMKCQAAIKAAEAAQKIAELEARKRENAERMADMEAEETKKALSGF 480

Query: 481 GHGDIGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRS 540
           GHGDIGYR+Y+I+EIE ATN FS SLKIGEGGYGPV+RG+LDHT VAIKVLRPDAA GRS
Sbjct: 481 GHGDIGYRKYTIEEIEIATNHFSDSLKIGEGGYGPVFRGKLDHTPVAIKVLRPDAAHGRS 540

Query: 541 QFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFR 600
           QFQ+EVEVLSCIRHPNMVLLLGACPENGCLVYEFM NGSLEDCLFRK  +PVL WQLRFR
Sbjct: 541 QFQQEVEVLSCIRHPNMVLLLGACPENGCLVYEFMGNGSLEDCLFRKANNPVLSWQLRFR 600

Query: 601 IAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQY 660
           IA EIATGLLFLHQ RPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVP SVA+SVTQY
Sbjct: 601 IAAEIATGLLFLHQHRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPLSVADSVTQY 660

Query: 661 RMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKF 720
           RMTATAGTF YIDPEYQ TGLLGI+SD+YSLGVMLLQI+T +PPIGLAH VERAI+KGKF
Sbjct: 661 RMTATAGTFCYIDPEYQLTGLLGIQSDIYSLGVMLLQIVTGRPPIGLAHAVERAIQKGKF 720

Query: 721 AEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQP 780
           AEMLDP+VKDWP E+ALKFAKLS++C EMRKRDRPDLGKVVLPEL+RLRAMAEESMGI+P
Sbjct: 721 AEMLDPEVKDWPFEDALKFAKLSMKCTEMRKRDRPDLGKVVLPELNRLRAMAEESMGIRP 780

Query: 781 Y---SICDSPCMSQASFSQEQFSLHQGLDES--ESSYCHSGSSTSS 810
           +   S   SP  SQ S SQE   +HQ    +  ESS   S SSTSS
Sbjct: 781 FNGSSASSSPYTSQTSSSQEPMRIHQEQCGAWVESSSSQSNSSTSS 816

BLAST of CmaCh12G009350 vs. ExPASy TrEMBL
Match: A0A061GQU4 (Kinase protein with adenine nucleotide alpha hydrolases-like domain, putative isoform 1 OS=Theobroma cacao OX=3641 GN=TCM_039769 PE=4 SV=1)

HSP 1 Score: 845.5 bits (2183), Expect = 1.9e-241
Identity = 492/839 (58.64%), Postives = 599/839 (71.39%), Query Frame = 0

Query: 3   RGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVG---A 62
           R    K+DESVAVAIDKDK SQ ALKW ++ L+SRGQ +TLLHVK KPS   T  G    
Sbjct: 4   RNNGEKRDESVAVAIDKDKSSQYALKWVVDHLMSRGQTITLLHVKTKPSSIPTPTGNHVN 63

Query: 63  CSDLDD--AVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNA 122
            SD++D  A A+KQQ+DNQA + FLPFRCFC+R +I CNE+ LE+ DI+KALI++   ++
Sbjct: 64  ISDVNDDVAKAYKQQLDNQAKEVFLPFRCFCSRKDIKCNEIILEDTDIAKALIDFVLSSS 123

Query: 123 IDVLVLGAPSRTGIVKRFK-SDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKP 182
           I+ LVLGAPS++G V+RF+ SD+P+ VSKGAPD+CTVY IG KGK+SS+R A++P P +P
Sbjct: 124 IETLVLGAPSKSGFVRRFRTSDVPTNVSKGAPDYCTVYVIG-KGKISSVRSASAPPPTRP 183

Query: 183 I-STSHSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHE 242
           +   +HSQ QPA+    + + T+A  A+  +P   R    +H           P R + E
Sbjct: 184 LPPRAHSQTQPAN----VLEPTDAPSATAYNPR-YRGDRPQH-----------PPRNMPE 243

Query: 243 DFEIKSPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSN 302
           D EI+SPF R   +       + S  D DISF+SSGR S DR     +++    + RLS 
Sbjct: 244 DMEIRSPFTRGHIK------YEPSIPDCDISFVSSGRPSTDRMFPSLYDNLEYGTPRLS- 303

Query: 303 GSGSELETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQS-- 362
              S+ +  S GS+         G RS+D +     S+ Y    SS     TSW+SQ+  
Sbjct: 304 -VSSDYDNRSFGSS-------YSGNRSMDFS-----SSQYELSSSSLESGRTSWSSQNMI 363

Query: 363 ----------HAQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEH 422
                       Q+DVEAEMRRL+ ELKQTMDMYS ACKEAL AKQ+A ELH WK++EE 
Sbjct: 364 QDRKSCSTVQMPQDDVEAEMRRLRQELKQTMDMYSAACKEALTAKQRAKELHSWKLQEEQ 423

Query: 423 RLEEARMAEGAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKK 482
           ++EEAR++E AAL++AEKEK KCKAA++AA+ AQR A  EA+KR N EK A  EA+E K 
Sbjct: 424 KIEEARLSEEAALSLAEKEKAKCKAAIEAAQAAQRIAELEAQKRINAEKKASKEADERKM 483

Query: 483 ALEGFGHGDIGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDA 542
           A +  GH D+ YRRY+I+EIE AT  FS S KIGEGGYGPVY+  LDHT VAIKVLRPDA
Sbjct: 484 AKKSLGH-DLRYRRYTIEEIEAATEHFSPSRKIGEGGYGPVYKCNLDHTPVAIKVLRPDA 543

Query: 543 AQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPW 602
           AQG SQF +EVEVLSCIRHPNMVLLLGACPE GCLVYE+MANG+LED LFR+   PVLPW
Sbjct: 544 AQGESQFHQEVEVLSCIRHPNMVLLLGACPEYGCLVYEYMANGNLEDRLFRRNNTPVLPW 603

Query: 603 QLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVAN 662
           Q+RFRIA EIATGLLFLHQT+PEP+VHRDLKP NILLDRNYVSKISDVGLARLVPPSVAN
Sbjct: 604 QVRFRIAAEIATGLLFLHQTKPEPLVHRDLKPANILLDRNYVSKISDVGLARLVPPSVAN 663

Query: 663 SVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAI 722
           SVTQY MT+TAGTF YIDPEYQQTG+LG KSD+YSLG++LLQIITAKPP+GL H+VE AI
Sbjct: 664 SVTQYHMTSTAGTFCYIDPEYQQTGMLGTKSDIYSLGILLLQIITAKPPMGLTHHVEHAI 723

Query: 723 EKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEES 782
           E G F  +LDP V DWPVEEAL FAKL+++C+E+R++DRPDLG+VVLPE +RLRA+ EE+
Sbjct: 724 ENGSFTGILDPAVLDWPVEEALIFAKLALKCSELRRKDRPDLGQVVLPEFNRLRALGEEN 783

Query: 783 MG-IQP-YSICDSPCMSQASFSQEQ------FSLHQGLDESESSYCHSGSSTSSYSDRR 815
           M  I P  S   SP  SQAS S  Q       SL  G D S S      S+TSS+  RR
Sbjct: 784 MPFITPGGSAGPSPNHSQASTSSNQDFLSDPQSLQSGYDSSRSR-----SNTSSFIGRR 799

BLAST of CmaCh12G009350 vs. ExPASy TrEMBL
Match: A0A5A7TVP5 (U-box domain-containing protein 34-like OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold648G00530 PE=4 SV=1)

HSP 1 Score: 845.1 bits (2182), Expect = 2.4e-241
Identity = 485/716 (67.74%), Postives = 548/716 (76.54%), Query Frame = 0

Query: 142 IVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTSHSQPQ-PASRPQLLHQLTNAS 201
           +VSKGAPDFCTVY I NKGKV +++Q+T+PAPAKP+++  +Q Q P  RPQLLHQLTNAS
Sbjct: 1   MVSKGAPDFCTVYIIANKGKV-TVKQSTAPAPAKPVASPRTQIQPPPPRPQLLHQLTNAS 60

Query: 202 KASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK-------------------- 261
           + S       +S     LETQ S+    P  +  ED++IK                    
Sbjct: 61  EVSSSETN--KSCSQSGLETQISI-KKVPLPSFQEDWDIKYVSSLALSHIHMCLYCYNRW 120

Query: 262 ------SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTR---DSY 321
                 SPF RPGSRA M K  D    DSDISF+SSGR+S D   S AWNS+     DS+
Sbjct: 121 RYIVGRSPFTRPGSRASMSKVMDPLMVDSDISFVSSGRRSTDYCLSGAWNSNIATGGDSF 180

Query: 322 RLSNGSGSELETSSPGSASPSPM-LGMGGMRSIDSNNNSSY---SNPYSHEMS-SASESE 381
           RLSN   SE E  SPG  SP  + +G G  +S+DSN N +Y   ++ +  +MS  +S SE
Sbjct: 181 RLSN--ASEFEVFSPGPTSPLMLEMGEGTNKSVDSNTNDNYNDNTHSFPQDMSFCSSPSE 240

Query: 382 T-SWASQSHAQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRL 441
           T SW S S  Q+DVEAEMRRLKLELKQTMDMYSTACKEA+ AKQKA ELHRWK+EEE RL
Sbjct: 241 TFSWPSHSQTQDDVEAEMRRLKLELKQTMDMYSTACKEAITAKQKAMELHRWKVEEEQRL 300

Query: 442 EEARMAEGAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKAL 501
           E ARMAE AALA+AEKEK+KCKAA++AAE AQR+A  EA+KRA VEK+  MEA+E K+ L
Sbjct: 301 EAARMAEEAALAMAEKEKIKCKAAMEAAEAAQRNAALEAKKRARVEKMVDMEAQEMKRTL 360

Query: 502 --EGFGHGDIGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDA 561
              G+G  +IGYR+Y+IQEIETAT GFS SLKIGEGGYGPV+RGELDHT VAIKVLRPDA
Sbjct: 361 SFSGYGQAEIGYRKYTIQEIETATKGFSDSLKIGEGGYGPVFRGELDHTPVAIKVLRPDA 420

Query: 562 AQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPW 621
           A GRSQFQ+EVEVLSCIRHPNMVLLLGACPE GCLVYEFMANGSLEDCLFRK  DP+L W
Sbjct: 421 AHGRSQFQQEVEVLSCIRHPNMVLLLGACPEKGCLVYEFMANGSLEDCLFRKANDPILSW 480

Query: 622 QLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVAN 681
           QLRFRIA EIATGLLFLHQTRPEPIVHRDLKPGNILLD NYVSKISDVGLARLVPPSVA+
Sbjct: 481 QLRFRIAAEIATGLLFLHQTRPEPIVHRDLKPGNILLDSNYVSKISDVGLARLVPPSVAD 540

Query: 682 SVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAI 741
           SVTQYRMTATAGTFFYIDPEYQQTGLLGIKSD+YSLGVMLLQIITA+PPIGLAH VERAI
Sbjct: 541 SVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDIYSLGVMLLQIITARPPIGLAHAVERAI 600

Query: 742 EKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEES 801
           EKGKFAE+LDP+VKDWPVE+ALKFAKLSI+CAEMRKRDRPDLGKVVLPEL+RLR MAEES
Sbjct: 601 EKGKFAEILDPEVKDWPVEDALKFAKLSIKCAEMRKRDRPDLGKVVLPELNRLRTMAEES 660

Query: 802 MGIQPY---SICDSPCMSQASFSQEQFSLHQGLDESESSYCHSGSSTSSYSDRRSW 817
           MG+  +   S   SP  SQ S         Q     E+S   S SSTSSY+  + +
Sbjct: 661 MGLGSFCCSSTSSSPYASQTSQEMMNSVRQQAGAWVETSSSQSDSSTSSYTREKPY 710

BLAST of CmaCh12G009350 vs. NCBI nr
Match: XP_022966072.1 (U-box domain-containing protein 52-like [Cucurbita maxima])

HSP 1 Score: 1529.2 bits (3958), Expect = 0.0e+00
Identity = 784/784 (100.00%), Postives = 784/784 (100.00%), Query Frame = 0

Query: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC 60
           MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC
Sbjct: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC 60

Query: 61  SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV 120
           SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV
Sbjct: 61  SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV 120

Query: 121 LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS 180
           LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS
Sbjct: 121 LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS 180

Query: 181 HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK 240
           HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK
Sbjct: 181 HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK 240

Query: 241 SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE 300
           SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE
Sbjct: 241 SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE 300

Query: 301 LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360
           LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE
Sbjct: 301 LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360

Query: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAE 420
           AEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAE
Sbjct: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAE 420

Query: 421 KEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSI 480
           KEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSI
Sbjct: 421 KEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSI 480

Query: 481 QEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCI 540
           QEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCI
Sbjct: 481 QEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCI 540

Query: 541 RHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFL 600
           RHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFL
Sbjct: 541 RHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFL 600

Query: 601 HQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYI 660
           HQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYI
Sbjct: 601 HQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYI 660

Query: 661 DPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWP 720
           DPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWP
Sbjct: 661 DPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWP 720

Query: 721 VEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPYSICDSPCMSQA 780
           VEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPYSICDSPCMSQA
Sbjct: 721 VEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPYSICDSPCMSQA 780

Query: 781 SFSQ 785
           SFSQ
Sbjct: 781 SFSQ 784

BLAST of CmaCh12G009350 vs. NCBI nr
Match: XP_023537969.1 (U-box domain-containing protein 52-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1514.2 bits (3919), Expect = 0.0e+00
Identity = 786/846 (92.91%), Postives = 796/846 (94.09%), Query Frame = 0

Query: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC 60
           MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVG  
Sbjct: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGTY 60

Query: 61  SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV 120
           SD DDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINY NKN IDV
Sbjct: 61  SDSDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYVNKNVIDV 120

Query: 121 LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS 180
           LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS
Sbjct: 121 LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS 180

Query: 181 HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK 240
           H+QP PA+RPQLLHQLTNAS+ SVEHP ILRSQ S+HLETQDS+ASNPPSR IHEDFEIK
Sbjct: 181 HNQPHPATRPQLLHQLTNASEVSVEHPIILRSQPSRHLETQDSVASNPPSRTIHEDFEIK 240

Query: 241 SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE 300
           SPFNRPGSRA MGKGQDTS ADSDISF+SSGRQSLDRGN PAWNS T DSYRLSNGS   
Sbjct: 241 SPFNRPGSRASMGKGQDTSMADSDISFVSSGRQSLDRGNPPAWNSGTVDSYRLSNGS--- 300

Query: 301 LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360
            ETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE
Sbjct: 301 -ETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360

Query: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQK----------------------------AS 420
           AEMRRLKLELKQTMDMYSTACKEALNAKQK                            AS
Sbjct: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQKVSIISKITNLFSMIYNHDSTIEFQLIWQAS 420

Query: 421 ELHRWKMEEEHRLEEARMAEGAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVEK 480
           ELHRWKMEEEHRLEEA+MAEGAALAIAEKEK+KCKAALQAAEVAQRSAVHEARKRANVEK
Sbjct: 421 ELHRWKMEEEHRLEEAKMAEGAALAIAEKEKMKCKAALQAAEVAQRSAVHEARKRANVEK 480

Query: 481 LAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDHT 540
           LAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSA+LKIGEGGYGPVYRGELDHT
Sbjct: 481 LAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSAALKIGEGGYGPVYRGELDHT 540

Query: 541 QVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCL 600
           QVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCL
Sbjct: 541 QVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCL 600

Query: 601 FRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVG 660
           FRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVG
Sbjct: 601 FRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVG 660

Query: 661 LARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPP 720
           LARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPP
Sbjct: 661 LARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPP 720

Query: 721 IGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPE 780
           IGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPE
Sbjct: 721 IGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPE 780

Query: 781 LSRLRAMAEESMGIQPYSICDSPCMSQASFSQEQFSLHQGLDESESSYCHSGSSTSSYSD 819
           LSRLRAMAEESMG QPYSICDSPCMSQASFSQE+FSLH GLDESESSYC SGSSTSSYSD
Sbjct: 781 LSRLRAMAEESMGFQPYSICDSPCMSQASFSQERFSLHHGLDESESSYCQSGSSTSSYSD 840

BLAST of CmaCh12G009350 vs. NCBI nr
Match: XP_022937745.1 (U-box domain-containing protein 52-like [Cucurbita moschata])

HSP 1 Score: 1513.4 bits (3917), Expect = 0.0e+00
Identity = 787/847 (92.92%), Postives = 798/847 (94.21%), Query Frame = 0

Query: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC 60
           MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVG+ 
Sbjct: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGSY 60

Query: 61  SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV 120
           SD DDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINY NKN IDV
Sbjct: 61  SDSDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYVNKNVIDV 120

Query: 121 LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS 180
           LVLGAPSRTG+VKRFKSDIPSIVSKGAPDFCTVY IGNKGKVSSMRQATSPAPAKPISTS
Sbjct: 121 LVLGAPSRTGLVKRFKSDIPSIVSKGAPDFCTVYIIGNKGKVSSMRQATSPAPAKPISTS 180

Query: 181 HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK 240
           H+QPQPA+RPQLLHQLTNAS+ SVEHPTILRSQ S+HLETQDSLASNPP RAIHEDFEIK
Sbjct: 181 HNQPQPATRPQLLHQLTNASEVSVEHPTILRSQPSRHLETQDSLASNPPFRAIHEDFEIK 240

Query: 241 SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE 300
           SPFNRPGSRA MGKGQDTSTADSDISF+SSGRQSLDRGNSPAWNS T DSYRLSNGS   
Sbjct: 241 SPFNRPGSRASMGKGQDTSTADSDISFVSSGRQSLDRGNSPAWNSGTGDSYRLSNGS--- 300

Query: 301 LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360
            ETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE
Sbjct: 301 -ETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360

Query: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQK-----------------------------A 420
           AEMRRLKLELKQTMDMYSTACKEALNAKQK                             A
Sbjct: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQKVSIISKISNLFSMIYNHDSTSNSNRLIWQA 420

Query: 421 SELHRWKMEEEHRLEEARMAEGAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVE 480
           SELHRWKMEEEHRLEEARMAE AALAIAE+EKLKCKAALQAAE+AQRSAV EARKRANVE
Sbjct: 421 SELHRWKMEEEHRLEEARMAEEAALAIAEQEKLKCKAALQAAELAQRSAVQEARKRANVE 480

Query: 481 KLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDH 540
           KLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSA+LKIGEGGYGPVYRGELDH
Sbjct: 481 KLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSAALKIGEGGYGPVYRGELDH 540

Query: 541 TQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDC 600
           TQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDC
Sbjct: 541 TQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDC 600

Query: 601 LFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDV 660
           LFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDV
Sbjct: 601 LFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDV 660

Query: 661 GLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKP 720
           GLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKP
Sbjct: 661 GLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKP 720

Query: 721 PIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLP 780
           PIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLP
Sbjct: 721 PIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLP 780

Query: 781 ELSRLRAMAEESMGIQPYSICDSPCMSQASFSQEQFSLHQGLDESESSYCHSGSSTSSYS 819
           ELSRLRAMAEESMG QP+SICDSPCMSQASFSQEQFSLHQGLDE ESSYC SGSSTSSYS
Sbjct: 781 ELSRLRAMAEESMGFQPFSICDSPCMSQASFSQEQFSLHQGLDECESSYCQSGSSTSSYS 840

BLAST of CmaCh12G009350 vs. NCBI nr
Match: KAG6586127.1 (U-box domain-containing protein 52, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1461.0 bits (3781), Expect = 0.0e+00
Identity = 758/819 (92.55%), Postives = 768/819 (93.77%), Query Frame = 0

Query: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC 60
           MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVG+ 
Sbjct: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGSY 60

Query: 61  SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV 120
           SD DDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINY NKN IDV
Sbjct: 61  SDSDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYVNKNVIDV 120

Query: 121 LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS 180
           LVLGAPSRTG+VKRFKSDIPSIVSKGAPDFCTVY IGNKGKVSSMRQATSPAPAKPISTS
Sbjct: 121 LVLGAPSRTGLVKRFKSDIPSIVSKGAPDFCTVYIIGNKGKVSSMRQATSPAPAKPISTS 180

Query: 181 HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK 240
           H+QPQPA+RPQLLHQLTNAS+ SVEHP ILRSQ S+HLETQDS+ASNPPSRAIHEDFEIK
Sbjct: 181 HNQPQPATRPQLLHQLTNASEVSVEHPIILRSQPSRHLETQDSVASNPPSRAIHEDFEIK 240

Query: 241 SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE 300
           SPFNRPGSRA  GKGQDTSTADSDISF+SSGRQSLDRGNSPAWNS T D YRLSNGSGSE
Sbjct: 241 SPFNRPGSRASTGKGQDTSTADSDISFVSSGRQSLDRGNSPAWNSGTGDLYRLSNGSGSE 300

Query: 301 LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360
            ETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMS ASESETSWASQSHAQEDVE
Sbjct: 301 FETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSFASESETSWASQSHAQEDVE 360

Query: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQK-----------------------------A 420
           AEMRRLKLELKQTMDMYSTACKEALNAKQK                             A
Sbjct: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQKVSIISKISNLFSMIYNHDSTSNSNRLIWQA 420

Query: 421 SELHRWKMEEEHRLEEARMAEGAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVE 480
           SELHRWKMEEEHRLEEARMAE AALAIAE+EKLKCKAALQAAEVAQRSAV EARKRANVE
Sbjct: 421 SELHRWKMEEEHRLEEARMAEEAALAIAEQEKLKCKAALQAAEVAQRSAVQEARKRANVE 480

Query: 481 KLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDH 540
           KLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSA+LKIGEGGYGPVYRGELDH
Sbjct: 481 KLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATNGFSAALKIGEGGYGPVYRGELDH 540

Query: 541 TQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDC 600
           TQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDC
Sbjct: 541 TQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDC 600

Query: 601 LFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDV 660
           LFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDV
Sbjct: 601 LFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDV 660

Query: 661 GLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKP 720
           GLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKP
Sbjct: 661 GLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKP 720

Query: 721 PIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLP 780
           PIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLP
Sbjct: 721 PIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLP 780

Query: 781 ELSRLRAMAEESMGIQPYSICDSPCMSQASFSQEQFSLH 791
           ELSRLRAMAEESMG QPYSICDSPCMSQASFSQE    H
Sbjct: 781 ELSRLRAMAEESMGFQPYSICDSPCMSQASFSQEAARHH 819

BLAST of CmaCh12G009350 vs. NCBI nr
Match: KAG7020948.1 (U-box domain-containing protein 35, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1382.1 bits (3576), Expect = 0.0e+00
Identity = 721/784 (91.96%), Postives = 731/784 (93.24%), Query Frame = 0

Query: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGAC 60
           MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVG+ 
Sbjct: 1   MARGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGSN 60

Query: 61  SDLDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDV 120
           SD DDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINY NKN IDV
Sbjct: 61  SDSDDAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYVNKNVIDV 120

Query: 121 LVLGAPSRTGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTS 180
           LVLGAPSRTG+VKRFKSDIPSIVSKGAPDFCTVY IGNKGKVSSMRQATSPAPAKPISTS
Sbjct: 121 LVLGAPSRTGLVKRFKSDIPSIVSKGAPDFCTVYIIGNKGKVSSMRQATSPAPAKPISTS 180

Query: 181 HSQPQPASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIK 240
           H+QPQPA+RPQLLHQLTNAS+ SVEHP ILRSQ               PSR         
Sbjct: 181 HNQPQPATRPQLLHQLTNASEVSVEHPIILRSQ---------------PSRR-------- 240

Query: 241 SPFNRPGSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSE 300
                          QDTSTADSDISF+SSGRQSLDRGNSPAWNS T D YRLSNGSGSE
Sbjct: 241 ---------------QDTSTADSDISFVSSGRQSLDRGNSPAWNSGTGDLYRLSNGSGSE 300

Query: 301 LETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360
            ETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE
Sbjct: 301 FETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVE 360

Query: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAE 420
           AEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWK+EEEHRLEEARMAE AALAIAE
Sbjct: 361 AEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKIEEEHRLEEARMAEEAALAIAE 420

Query: 421 KEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSI 480
           +EKLKCKAALQAAEVAQRSAV EARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSI
Sbjct: 421 QEKLKCKAALQAAEVAQRSAVQEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSI 480

Query: 481 QEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCI 540
           QEIETATNGFSA+LKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCI
Sbjct: 481 QEIETATNGFSAALKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCI 540

Query: 541 RHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFL 600
           RHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPE+PVLPWQLRFRIAEEIATGLLFL
Sbjct: 541 RHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPENPVLPWQLRFRIAEEIATGLLFL 600

Query: 601 HQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYI 660
           HQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYI
Sbjct: 601 HQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYI 660

Query: 661 DPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWP 720
           DPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWP
Sbjct: 661 DPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWP 720

Query: 721 VEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPYSICDSPCMSQA 780
           VEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMG QPYSICDSPCMSQA
Sbjct: 721 VEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGFQPYSICDSPCMSQA 746

Query: 781 SFSQ 785
           SFSQ
Sbjct: 781 SFSQ 746

BLAST of CmaCh12G009350 vs. TAIR 10
Match: AT2G24370.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 770.4 bits (1988), Expect = 1.5e-222
Identity = 439/786 (55.85%), Postives = 551/786 (70.10%), Query Frame = 0

Query: 13  VAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPS----HSTTNVGACSDLDDAVA 72
           VAVAIDKDK SQ ALKW ++ LL RGQ + L+HVK +PS     ++ +  +     D+  
Sbjct: 20  VAVAIDKDKSSQHALKWAVDNLLQRGQSVILVHVKLRPSPLNNSASLHASSAKLSQDSSL 79

Query: 73  HKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDVLVLGAPSR 132
             +  +  + + FLPFRCFCTR +I C +V LEE D++KAL+ Y N+ AI+VLV+G+ S+
Sbjct: 80  VCRDPEGISKEIFLPFRCFCTRKDIQCQDVLLEESDVAKALVEYVNQAAIEVLVVGSSSK 139

Query: 133 TGIVKRFK-SDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTSHSQPQPA 192
            G ++  K +DIP  ++K APDFCTVY I +KGK+ +MR A+  AP      S  QP P+
Sbjct: 140 GGFLRFNKPTDIPGSITKNAPDFCTVYII-SKGKIQTMRSASRSAPMTAPLRSSVQP-PS 199

Query: 193 SRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFE-IKSPFNRP 252
            +P      T+A+    +  +   +Q     + Q     +   R+I +  E  +SPF R 
Sbjct: 200 LKPPQPMPSTSANSLRADRRSFESNQRRSVEDQQRRSMEDQQRRSIDDQSESFRSPFTRR 259

Query: 253 GSRALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAW--NSSTRDSYRLSNGSGSELETS 312
           G+    G   D +  +SDISF+SSGR S+DR     +  N  +R   RLSN S  +  +S
Sbjct: 260 GNGRSYG---DLTVPESDISFISSGRPSIDRIFPSLYDNNDPSRTPPRLSNFSDMDYSSS 319

Query: 313 SPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVEAEMR 372
              S++        G RS+D N+ + +    S   SSAS+S           +DVEAEMR
Sbjct: 320 LDQSSN-------YGRRSVDMNSPTDFE---SERFSSASQS----------IDDVEAEMR 379

Query: 373 RLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAEKEKL 432
           RLKLELKQTM+MYSTACKEAL AKQKA+EL RWK+EEE +LEEAR AE AALAIAEKEK 
Sbjct: 380 RLKLELKQTMEMYSTACKEALTAKQKATELQRWKLEEERKLEEARNAEEAALAIAEKEKA 439

Query: 433 KCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSIQEIE 492
           K KAA++AAE AQR A  EA+KR N E  A+ E+EE  KAL    + D+ YR+YSI++IE
Sbjct: 440 KSKAAMEAAEAAQRIAELEAKKRVNAEMKALKESEEKTKALTALANSDVRYRKYSIEDIE 499

Query: 493 TATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPN 552
            AT  F+   KIGEGGYGPVY+  LDHT VA+KVLRPDAAQGRSQFQ+EVEVLSCIRHPN
Sbjct: 500 LATEFFAEKYKIGEGGYGPVYKCYLDHTPVAVKVLRPDAAQGRSQFQQEVEVLSCIRHPN 559

Query: 553 MVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTR 612
           MVLLLGACPE GCLVYEFMANGSLED LFR    P L WQ+RFRIA EI TGLLFLHQ +
Sbjct: 560 MVLLLGACPECGCLVYEFMANGSLEDRLFRLGNSPPLSWQMRFRIAAEIGTGLLFLHQAK 619

Query: 613 PEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYIDPEY 672
           PEP+VHRDLKPGNILLDRN+VSKISDVGLARLVPP+VA++VTQYRMT+TAGTF YIDPEY
Sbjct: 620 PEPLVHRDLKPGNILLDRNFVSKISDVGLARLVPPTVADTVTQYRMTSTAGTFCYIDPEY 679

Query: 673 QQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEA 732
           QQTG+LG+KSD+YSLG+M LQ+ITAKPP+GL H VERA+EKG   ++LDP V DWP+E+ 
Sbjct: 680 QQTGMLGVKSDIYSLGIMFLQLITAKPPMGLTHYVERALEKGTLVDLLDPVVSDWPMEDT 739

Query: 733 LKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMG--IQPYSICDSPCMSQASF 789
            +FAKL+++CAE+R++DRPDL KV+LPEL+RLR +A+ES    +   S   SP  SQ+S 
Sbjct: 740 EEFAKLALKCAELRRKDRPDLAKVILPELNRLRTLADESSHSVVVGNSPLPSPTGSQSSL 780

BLAST of CmaCh12G009350 vs. TAIR 10
Match: AT4G31230.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 719.2 bits (1855), Expect = 3.9e-207
Identity = 417/775 (53.81%), Postives = 528/775 (68.13%), Query Frame = 0

Query: 3   RGGAHKKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGACSD 62
           R G    D  VAVAID+DK SQ ALKW ++ LL +GQ + L+HVK + S  +TN    S+
Sbjct: 8   RDGGFGVDGLVAVAIDRDKNSQGALKWAVDNLLQKGQTVVLVHVKPRASSLSTNPSINSN 67

Query: 63  LD-------DAVAHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANK 122
                    D+     + +    Q FLPFRC C+R +I C +V LEE D+++AL+ YAN+
Sbjct: 68  SSKTSQINGDSSLVCGEPEGSYKQLFLPFRCLCSRKDIQCKDVLLEESDVARALVEYANQ 127

Query: 123 NAIDVLVLGAPSRTGIVKRFKS-DIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPA 182
             I+VLV+G+ S+ G ++  K  DIP  ++K APDFCTVY I  KGK+S+ + A+  AP+
Sbjct: 128 VMIEVLVVGSSSKGGFLRFNKPIDIPGAITKTAPDFCTVYAI-TKGKLSTKKTASRAAPS 187

Query: 183 -KPISTSHSQPQPASRPQLLHQLTN--ASKASVEHPTILRSQHSKHLETQDSLASNPPSR 242
             P+     Q      P L    TN  A + S E      SQH + L+ Q          
Sbjct: 188 VSPLRIQLQQNGLKPHPPLPSATTNTRAERQSFE------SQHRRSLDDQSD-------- 247

Query: 243 AIHEDFEIKSPFNRPGSRALMGKGQ-DTSTADSDISFMSSGRQSLDRGNSPAWNSS--TR 302
                   + P+N+   R L G+   + S  DS+ISF SSGR S++R +   +++S   R
Sbjct: 248 ------SFRPPYNK---RGLTGRSYGELSIPDSEISFNSSGRPSIERNSPSLYDNSDPNR 307

Query: 303 DSYRLSNGSGSELETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETS 362
              RLSN   S+++  S  S +        G RS+D ++ +++        S+ S     
Sbjct: 308 TPPRLSN--FSDVDYCSFESMT-------FGRRSMDLSSPTAF--------STGSFENER 367

Query: 363 WASQSHAQEDVEAEMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEA 422
           ++S S   +DVEAEMRRLKLELKQTM+MYSTACKEAL AK KA+EL RWK+ EE + EEA
Sbjct: 368 FSSASQGGDDVEAEMRRLKLELKQTMEMYSTACKEALTAKHKATELQRWKLAEERKFEEA 427

Query: 423 RMAEGAALAIAEKEKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGF 482
           ++AE AALAIAEKEK K KAA++AAE AQR A  E+RKR + E  A+ E+E   KA+   
Sbjct: 428 KLAEEAALAIAEKEKAKSKAAMEAAEAAQRIADIESRKRVDAETKALKESEARTKAVNAL 487

Query: 483 GHGDIGYRRYSIQEIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRS 542
              D+ YR+YSI+EIE AT  F    KIGEG YGPVY+  LDHT VA+K LRPDAAQGRS
Sbjct: 488 AK-DVRYRKYSIEEIEDATEFFDDKYKIGEGSYGPVYKCYLDHTPVAVKALRPDAAQGRS 547

Query: 543 QFQREVEVLSCIRHPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFR 602
           QFQ+EVEVL  IRHPNMVLLLGACPE GCLVYEFMANGSLED LFR+ + P L WQ RFR
Sbjct: 548 QFQKEVEVLCSIRHPNMVLLLGACPECGCLVYEFMANGSLEDRLFRQGDSPALSWQTRFR 607

Query: 603 IAEEIATGLLFLHQTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQY 662
           IA EI T LLFLHQT+PEP+VHRDLKP NILLDRN+VSK++DVGLARLVPPSVAN+VTQY
Sbjct: 608 IAAEIGTVLLFLHQTKPEPLVHRDLKPANILLDRNFVSKLADVGLARLVPPSVANTVTQY 667

Query: 663 RMTATAGTFFYIDPEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKF 722
            MT+TAGTF YIDPEYQQTG+LG+KSD+YSLG+M LQ+IT KPP+GL H VERA+EKG  
Sbjct: 668 HMTSTAGTFCYIDPEYQQTGMLGVKSDIYSLGIMFLQLITGKPPMGLTHYVERALEKGNL 727

Query: 723 AEMLDPDVKDWPVEEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEES 764
            ++LDP V DWPVE+  +FAKL+++CAE+R++DRPDL KV+LPEL+RLR +AEES
Sbjct: 728 KDLLDPAVSDWPVEDTTEFAKLALKCAEIRRKDRPDLSKVILPELNRLRVLAEES 740

BLAST of CmaCh12G009350 vs. TAIR 10
Match: AT1G78940.2 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 701.8 bits (1810), Expect = 6.4e-202
Identity = 417/784 (53.19%), Postives = 533/784 (67.98%), Query Frame = 0

Query: 12  SVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGACSDLDDAV-AHK 71
           SVAVAIDKDK SQ ALKWTI+ L SRGQ ++L+HV  + SHS+      SDL++     K
Sbjct: 17  SVAVAIDKDKGSQHALKWTIDNLASRGQTISLIHVLCR-SHSS------SDLEEGTPQQK 76

Query: 72  QQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDVLVLGAPSRTG 131
           QQ++  A   F+ F C+C+R  I C ++ LE+ D  +A+  Y + +AI+ LV+G+ SR G
Sbjct: 77  QQMEKIAKDLFVSFHCYCSRKEINCRDILLEDADKVRAITEYVSSSAIENLVVGSASRNG 136

Query: 132 IVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTSHSQPQPASRP 191
            ++RFK+D+P+ VSK APDFC VY I +KGK++S+R A+ PAP +     +S  Q     
Sbjct: 137 FMRRFKTDLPTTVSKSAPDFCNVYVI-SKGKIASVRNASRPAPYQ-----NSMQQCEIDN 196

Query: 192 QLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIKSPFNRPGSRA 251
              H    A K   +HP    S  S+  ++ ++  S    R  + DF          S +
Sbjct: 197 HHPHTPDKAPKYH-DHPNSAGSTPSRPRKSVEADRSPLVKRKPYGDFY--------DSDS 256

Query: 252 LMGKGQDTSTADSDISFMSSGRQSLDRGN-SPAWNSSTRDSYRLSNGSGSELETSSPGSA 311
            +     +S  DSDISF+SSGR S++R + S  +  S R S R+S  S   + ++  G  
Sbjct: 257 DLSFISPSSHRDSDISFISSGRPSVERSSFSLDFPESARTS-RMSTSSEQSIGSNRLGIK 316

Query: 312 SPSP-MLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVEAEMRRLKL 371
              P  L      S +S   SSYS                    S + +DVEAEM+RL+L
Sbjct: 317 FSDPDFLNESSTFSEESGRTSSYS--------------------SQSLDDVEAEMKRLRL 376

Query: 372 ELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAEKEKLKCKA 431
           ELKQTMDMYSTACKEAL+A+Q+A+EL + + EEE RLEEA+ +E AA++I EKE+ K KA
Sbjct: 377 ELKQTMDMYSTACKEALSARQQATELQKLRTEEERRLEEAKSSEEAAMSIVEKERAKAKA 436

Query: 432 ALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETATN 491
           AL+AAE A+R A  E+++R   E        +T K  + F  G + YR+Y++ EIE AT+
Sbjct: 437 ALEAAEAAKRLAEVESKRRLTAEM-------KTMKESDSFSRGFVRYRKYTVDEIEEATS 496

Query: 492 GFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVLL 551
            F+ S K+GEGGYGPV+RG LDHT VA+KVLRPDAAQGRSQFQ+EVEVLSCIRHPNMVLL
Sbjct: 497 NFAESQKVGEGGYGPVFRGFLDHTSVAVKVLRPDAAQGRSQFQKEVEVLSCIRHPNMVLL 556

Query: 552 LGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEPI 611
           LGACPE G LVYE+MA GSLED LF +   P + WQLRFRIA EIATGLLFLHQT+PEPI
Sbjct: 557 LGACPEFGILVYEYMAKGSLEDRLFMRGNTPPITWQLRFRIAAEIATGLLFLHQTKPEPI 616

Query: 612 VHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQTG 671
           VHRDLKPGN+LLD NYVSKISDVGLARLV P+VA +VTQYR+T+ AGTF YIDPEYQQTG
Sbjct: 617 VHRDLKPGNVLLDYNYVSKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQTG 676

Query: 672 LLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKFA 731
           +LG+KSDVYSLG+MLLQI+TAK P+GLA+ VE+AIE+G   +MLDP V DWP+EEAL  A
Sbjct: 677 MLGVKSDVYSLGIMLLQILTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPDWPIEEALSLA 736

Query: 732 KLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGIQPY--SICDSPCMSQASFSQEQ 791
           KLS+QCAE+R++DRPDLGK +LPEL+RLR + EES+    Y  +   SP  SQA    EQ
Sbjct: 737 KLSLQCAELRRKDRPDLGKEILPELNRLREIGEESLESVFYAGNQGKSPNTSQAHNGFEQ 749

BLAST of CmaCh12G009350 vs. TAIR 10
Match: AT1G16760.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 698.7 bits (1802), Expect = 5.4e-201
Identity = 394/757 (52.05%), Postives = 510/757 (67.37%), Query Frame = 0

Query: 9   KDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGACSDLDDAVA 68
           ++ SVA+AIDKDK SQ A+KWT+E L +RGQ L L+HV  K   S        D+++ + 
Sbjct: 14  RNGSVAIAIDKDKSSQNAIKWTLENLATRGQTLALIHVLPKSQSSL-------DIEEGIT 73

Query: 69  HKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDVLVLGAPSR 128
           HKQQ++ Q    F+ F C+C+R  I C +V LE+ D  KA++ Y   +AI+ LVLGAPSR
Sbjct: 74  HKQQMEKQTKDLFVSFHCYCSRKEIHCLDVVLEDVDKVKAIVEYVTVSAIENLVLGAPSR 133

Query: 129 TGIVKRFKSDIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTSHSQPQPAS 188
              ++RFK+D+P+ VSK APDFC VY I +KGK+SS+R A+  AP  P   S        
Sbjct: 134 NSFMRRFKTDLPTSVSKAAPDFCNVYVI-SKGKISSLRNASRLAPYHPSVLSEVDDHETI 193

Query: 189 RPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIKSPFNRPGS 248
             +  H+  N        P + + + S   +        PP    H   ++   F+   S
Sbjct: 194 AIERKHKTANT-------PALPKGRRSIDSDVTRLGLPKPP----HGHMKLMGDFSDSES 253

Query: 249 R-ALMGKGQDTSTADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSELETSSPG 308
             + +   Q  S   SD+SF+SSGR S+DR +          +Y L   + +   ++S  
Sbjct: 254 EFSFISASQQES---SDLSFISSGRPSVDRSSF---------TYDLPESARTSRMSTSSE 313

Query: 309 SASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVEAEMRRLK 368
            +  S  LG+          + SY N  S     +  +  S++SQS    DVEA+M+RL+
Sbjct: 314 QSIGSHRLGI-------KFTDLSYLNGSSSVSDESGRTSCSFSSQS--LNDVEAQMKRLR 373

Query: 369 LELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAEKEKLKCK 428
           LELKQTMDMYS+AC+EAL A+ +A+EL + + EEE RLEE +M E  A++I E E+ K K
Sbjct: 374 LELKQTMDMYSSACREALTARNEATELQKLRTEEERRLEELKMTEETAMSIVENERAKAK 433

Query: 429 AALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSIQEIETAT 488
            AL+AAE A R A  EA++R + E   + E++   +      H  + YR+YS+QEIE  T
Sbjct: 434 TALEAAEAANRLAEVEAKRRVHAEMKVLKESDSFSR------HSIVRYRKYSVQEIEEGT 493

Query: 489 NGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCIRHPNMVL 548
             F+ S K+GEGGYGPV+RG LDHT VA+KVLRPDAAQGRSQF +EVEVLSCIRHPNMVL
Sbjct: 494 ANFAESRKVGEGGYGPVFRGHLDHTSVAVKVLRPDAAQGRSQFHKEVEVLSCIRHPNMVL 553

Query: 549 LLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFLHQTRPEP 608
           LLGACPE G LVYE+MA GSL+D LFR+   P + WQLRFRIA EIATGLLFLHQT+PEP
Sbjct: 554 LLGACPEYGILVYEYMARGSLDDRLFRRGNTPPISWQLRFRIAAEIATGLLFLHQTKPEP 613

Query: 609 IVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYIDPEYQQT 668
           IVHRDLKPGN+LLD NYVSKISDVGLARLV P+VA +VTQYR+T+ AGTF YIDPEYQQT
Sbjct: 614 IVHRDLKPGNVLLDHNYVSKISDVGLARLV-PAVAENVTQYRVTSAAGTFCYIDPEYQQT 673

Query: 669 GLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWPVEEALKF 728
           G+LG+KSDVYSLG+MLLQ++TAK P+GLA+ VE+AIE+G   +MLDP V DWP+EEAL  
Sbjct: 674 GMLGVKSDVYSLGIMLLQLLTAKQPMGLAYYVEQAIEEGTLKDMLDPAVPDWPLEEALSL 723

Query: 729 AKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESM 765
           AKLS+QCAE+R++DRPDLGK V+PELSRLR + EES+
Sbjct: 734 AKLSLQCAELRRKDRPDLGKEVMPELSRLREIGEESL 723

BLAST of CmaCh12G009350 vs. TAIR 10
Match: AT2G07020.1 (Protein kinase protein with adenine nucleotide alpha hydrolases-like domain )

HSP 1 Score: 690.3 bits (1780), Expect = 1.9e-198
Identity = 395/765 (51.63%), Postives = 505/765 (66.01%), Query Frame = 0

Query: 8   KKDESVAVAIDKDKFSQQALKWTIERLLSRGQVLTLLHVKHKPSHSTTNVGACSDLDDAV 67
           ++DESVA+AID+DK SQ ALKW ++ LL+ G+ LTL+HVK K + +          DD  
Sbjct: 9   RRDESVAIAIDRDKGSQAALKWAVDNLLTPGETLTLIHVKVKQTLANNGTQPNKSGDD-- 68

Query: 68  AHKQQVDNQAHQFFLPFRCFCTRSNIPCNEVTLEEGDISKALINYANKNAIDVLVLGAPS 127
                      + FLPFRCFCTR +I C EV LE  D ++ +I Y  +NAID+LVLGA S
Sbjct: 69  ---------VKELFLPFRCFCTRKDINCEEVVLENVDAAEGIIEYVQENAIDILVLGA-S 128

Query: 128 RTGIVKRFKS-DIPSIVSKGAPDFCTVYTIGNKGKVSSMRQATSPAPAKPISTSHSQPQP 187
           +  ++KR K+ D+ + V KGAP+FCTVY I +KGK+SS+R ATS  P          P  
Sbjct: 129 KITLLKRLKAVDVTNAVIKGAPNFCTVYAI-SKGKISSVRSATSSPP----------PLC 188

Query: 188 ASRPQLLHQLTNASKASVEHPTILRSQHSKHLETQDSLASNPPSRAIHEDFEIKSPFNRP 247
             RPQL  + +NA+  +       R Q                 ++I ++ EIKSP+ R 
Sbjct: 189 TIRPQLPARSSNANNNNFSPRAQRRLQ---------------SVQSIQDEIEIKSPYLR- 248

Query: 248 GSRALMGKGQDTST-----ADSDISFMSSGRQSLDRGNSPAWNSSTRDSYRLSNGSGSEL 307
                  K  D  T     +DSD+SF+SS R S+D      W    R +Y  S+ S  + 
Sbjct: 249 -------KEYDQGTYQALVSDSDLSFVSSDRPSMD------WFEDNRSNYATSSSSSEK- 308

Query: 308 ETSSPGSASPSPMLGMGGMRSIDSNNNSSYSNPYSHEMSSASESETSWASQSHAQEDVEA 367
                              +SID        + YS   +S+ ES    +   ++Q+DVE+
Sbjct: 309 -------------------QSID------LCSSYSAFSTSSQESGRLSSLSMYSQDDVES 368

Query: 368 EMRRLKLELKQTMDMYSTACKEALNAKQKASELHRWKMEEEHRLEEARMAEGAALAIAEK 427
           EMRRLKLELK TMDMY++ACKEA+ AK+   ELH+WK E +H+LEEA +A+ AA+AIAE 
Sbjct: 369 EMRRLKLELKYTMDMYNSACKEAIAAKKTTIELHKWKEERKHKLEEAILAKEAAMAIAEN 428

Query: 428 EKLKCKAALQAAEVAQRSAVHEARKRANVEKLAVMEAEETKKALEGFGHGDIGYRRYSIQ 487
           EK K +AA++A E A R A  EA+KR  +E  A+ E E   KA+    H +  YR+Y+I+
Sbjct: 429 EKAKSRAAMEALEAAHRMAETEAQKRKQIETAALREVEHENKAMHALPHSNRMYRKYTIE 488

Query: 488 EIETATNGFSASLKIGEGGYGPVYRGELDHTQVAIKVLRPDAAQGRSQFQREVEVLSCIR 547
           EIE  T  FS S KIGEG YG VY+G LD+T VAIKV+RPDA QGRSQFQ+EVEVL+CIR
Sbjct: 489 EIEQGTTKFSDSHKIGEGSYGTVYKGTLDYTPVAIKVVRPDATQGRSQFQQEVEVLTCIR 548

Query: 548 HPNMVLLLGACPENGCLVYEFMANGSLEDCLFRKPEDPVLPWQLRFRIAEEIATGLLFLH 607
           HPNMVLLLGAC E GCLVYE+M+NGSL+DCL R+   PVL WQLRFRIA EIAT L FLH
Sbjct: 549 HPNMVLLLGACAEYGCLVYEYMSNGSLDDCLLRRGNSPVLSWQLRFRIAAEIATSLNFLH 608

Query: 608 QTRPEPIVHRDLKPGNILLDRNYVSKISDVGLARLVPPSVANSVTQYRMTATAGTFFYID 667
           Q +PEP+VHRDLKP NILLD++ VSKISDVGLARLVPP++ +  T YRMT+TAGT  YID
Sbjct: 609 QLKPEPLVHRDLKPANILLDQHMVSKISDVGLARLVPPTIDDIATHYRMTSTAGTLCYID 668

Query: 668 PEYQQTGLLGIKSDVYSLGVMLLQIITAKPPIGLAHNVERAIEKGKFAEMLDPDVKDWPV 727
           PEYQQTG+LG KSD+YS G++LLQI+TAK P+GL + VE+AIE+G FA++LDP V DWP+
Sbjct: 669 PEYQQTGMLGTKSDIYSFGIVLLQILTAKTPMGLTNQVEKAIEEGNFAKILDPLVTDWPI 695

Query: 728 EEALKFAKLSIQCAEMRKRDRPDLGKVVLPELSRLRAMAEESMGI 767
           EEAL  AK+ +QCAE+R++DRPDLG VVLP L RL  +AEE+M I
Sbjct: 729 EEALILAKIGLQCAELRRKDRPDLGTVVLPGLKRLMNLAEENMSI 695