CmaCh12G008270 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G008270
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	WAT1-related protein
Location	Cma_Chr12: 6097076 .. 6100160 (+)
RNA-Seq Expression	CmaCh12G008270
Synteny	CmaCh12G008270

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

GAAAGAAGGCCTCTAAGGAAGAGAGAGGGGAGATGGGTGTGGTTGAAGAGTACTTGCCAGCAATGGCGATGTTGGGAATTCAAGCAATTTATGCCATTGTCACACTCATCTCTAGGGCTGCCCTGTTGGAGGGTATGAGTCCTAGAGTGTTCATCGTTTATAGGCAGGCCTTTGCCACTCTCTCTATTGCACCCATTGCTTATCTCTCCAGGTTAGATGTGCATTCTTCATTCTCAAACACTATCTTGGTTCTTTTCTGTTTTTTGTTTGTCTTATAATTTGGGGATGGCAGGTCGAAATCCATGAAGGTGTCTTTGGACCTCAAGAGCTTTTATCTCATCTTTTTTGCTGCACTTATCGGGTAAGATTTAATAGGGATGTTTATGGGTTCGGTTAGCGTGAGGGAAAAATCTTTTGGACCAACAAAAAAAAAAAAAATTCTAAATAGGTTAGGATGGTTAGTTGGGTTTGATTGGATTGTCAGGTTTTCTTGGAGGCGTATAGAGCCCTCCAACAAAGTACACTATAGAGCCTCCTCTAAGCCCTATAAAGTTGTCAAACAGTCTCCTTTAATCGAGGCTCGACCTCTTGAACAAAGTACACCCTTTGATTGACATGGTCAAGTTAAGGGCATGACTCTAATACCATCAAACAACCTCCCCTTAATCTAGACTCTACTACTAAAAAAAAATACAACCATTTGTTTGACACATGAGGATTCTATCATGGCCTTCTATAATTGTATGATATTGTCCTCTTTTAGCATAACTTATAACGACTTTGCTTTGAGTTTCTCCAAAAATACTCGGGAGATGTATTCCTTACCTATAAACTCATAATCATTCCCTAAATTAGTCAATGCGTGACTCCCTCCCAACAATTCTCAACACTACACACTACATTTTACATTAATAACTAAAATTTCGTAAAACTCAAATATCAAATATTTCTAGTTACAACATATCACTAATATTTGTTACAAATGTCAAATATTCTTAATTTTCATGATAATGGGTATGGATGGATTGTAATTTTATTTTTGGGTTGGCCAAGTTGATCCGACAAAGAAAATGTCAATCTAAGACAACCCGAATTTTTGATCTTTCATGAATTCAACTCAACTAAACCCAACCTAACTTTTACCGTTTAGATTTGATAGTCGGAGTTGATGAGATTCAAAAGTTATTTAAACATGCCTAAGCTTTTGGCTAATTTTGGGTGTTTTGGACAGTTCAACCATGAACCACAATTTTTTCTACGAGGGACTGTTCTTAGCCAGTTCATCATTAGCAACCGCGATGGAGAATCTGATTCCGGCAGTGACATTTATAATAGCAGCAATGGTCGGGTGAGTCAGTGGTAATATTATATTAAACCATTAAGTTTAGTAATAATTTATGTAACGACCCAGATGTACTTCTCTTTAGGCAAAACTTTAAAACGCATTTGTTAGGAGAAGGTTTTCGACATCACAATTTAACAATCGAAAATTCGCAGAATGGAGAGCGTGAAGCTGACAAACTTGAGGTGCATGGCTAAGATTGGAGGTACAGTGGTTTGTGTGGGTGGGGCTATGTTCATGGCCCTTCTAAGAGGTCCAAAGCTTCTGAATGCCACACAAGCTTTTGGTGTCAAATCTGCAATATTTGGTGTGGAAAGTGGAAGCGATGAGGCTTGGTTGTTGGGGTCACTCAGTCTGTTTGGGAGCTGTTGCTGTTGGTCAATTTGGTTGATATTGCAGGTGCCGGCAATGGCAAGCTATCCTGACAAGCTATCCTTATCGGCATGGACTTGTTTTTTTTCACTCATACAATCAGTGGGTTTCACATTATGGGTAGAGGGAGCCAATGTGGAAACGTGGAAGATTCATTCAAAGACTGAACTTGTTTGTTACCTGTTCTCAGTAAGTAATTTTTTACAATTACCCAATTTTAGAGGTCATTTTGAGATCTAATTGTGTTTGGTTCTTAGGGGATTTTTGGGTCAGGAGTTGCCTACTTTCTTCAAGCTTGGGGTATTTCCAAGAGGGGACCTGTCTTCTCTGCAGTGTTCAACCCTTTTTGCACCATTATTACCACTATTTTGGCAGCTATATTTCTTCATGAGGAGATTTATACCGGAAGGTAAATTTTATATATGCGTTAATTTAGATATATATTTTTTTTTTAATAATTTTGATTATCATGAAGCCACGTATTAGGGTTTGGTATTCTTTTCTCGACTCAAATGGGTTGAGAATTTTATCGATTTCATTCAAAAACGGGGTTTTTTTTTTTTTTGTTGGGTTAATATGACATCAGAGTAAAATAAGAGTATATAGATGAATAGAGACCACATTTAAATGAGAGTAATCAGGAGACTATGATGATAAAATGTGATATAATATACTGGTTGTGGAGGAAAACAAAACACCCTTTGTAAGGGTGTGGAAACCTTTCACTAGTATACGTGTTTTAAAGTTGTGAGGAAAAGCCTGAAAAAGAAAATCCAAAAAGAACAATATCTACTAGCGGTGTACTTGTGCTGTTATATAAAAAGCATGTGAGTGAAAACAATTCACTCTCAGAAGCAATTTGAGATAAGTAAGTAGCATGAATAAATGTTTGGGTTAATTTTTGGGATGAGAAACGAGAAACGAGAAACGAGAAACTCGTCGAAACCTAAAATCGTTACTCTGTTATTCGTAAGTGTTTTTTTTTTTTTTGAATAATTTTCAGCTTGTTGGGGGGCGTGATTGTAATTATTGGATTATATGTGGTTCTTTGGGGGAAAACAAATGATTATACGAAGGAAGAAGACAGAGAAAAGTGTAGAGTTGAAACACAAGAAGAGGATTGTGAATCAGCTTCAACTTCGATTGAGACAGATAGCTATAAAGTTGATGTGAGGGAACCCCTTTTGTCGTCTGGGTGTACACATCATATTGACAGCTAGGCAAACGGATCTACGACATATGCCACGTGGCATTTATGTATGCTCCACGTGTTCTCCAAGAAAAAAGGAAAAATATGTGTGAGATATATTTTTTTATTCTTGAACCTTTTTGTTTTCTTTTGTTTTAATCTTTACGAGTTTTAAGCTTAAT

mRNA sequence

GAAAGAAGGCCTCTAAGGAAGAGAGAGGGGAGATGGGTGTGGTTGAAGAGTACTTGCCAGCAATGGCGATGTTGGGAATTCAAGCAATTTATGCCATTGTCACACTCATCTCTAGGGCTGCCCTGTTGGAGGGTATGAGTCCTAGAGTGTTCATCGTTTATAGGCAGGCCTTTGCCACTCTCTCTATTGCACCCATTGCTTATCTCTCCAGGTCGAAATCCATGAAGGTGTCTTTGGACCTCAAGAGCTTTTATCTCATCTTTTTTGCTGCACTTATCGGTTCAACCATGAACCACAATTTTTTCTACGAGGGACTGTTCTTAGCCAGTTCATCATTAGCAACCGCGATGGAGAATCTGATTCCGGCAGTGACATTTATAATAGCAGCAATGGTCGGAATGGAGAGCGTGAAGCTGACAAACTTGAGGTGCATGGCTAAGATTGGAGGTACAGTGGTTTGTGTGGGTGGGGCTATGTTCATGGCCCTTCTAAGAGGTCCAAAGCTTCTGAATGCCACACAAGCTTTTGGTGTCAAATCTGCAATATTTGGTGTGGAAAGTGGAAGCGATGAGGCTTGGTTGTTGGGGTCACTCAGTCTGTTTGGGAGCTGTTGCTGTTGGTCAATTTGGTTGATATTGCAGGTGCCGGCAATGGCAAGCTATCCTGACAAGCTATCCTTATCGGCATGGACTTGTTTTTTTTCACTCATACAATCAGTGGGTTTCACATTATGGGTAGAGGGAGCCAATGTGGAAACGTGGAAGATTCATTCAAAGACTGAACTTGTTTGTTACCTGTTCTCAGGGATTTTTGGGTCAGGAGTTGCCTACTTTCTTCAAGCTTGGGGTATTTCCAAGAGGGGACCTGTCTTCTCTGCAGTGTTCAACCCTTTTTGCACCATTATTACCACTATTTTGGCAGCTATATTTCTTCATGAGGAGATTTATACCGGAAGCTTGTTGGGGGGCGTGATTGTAATTATTGGATTATATGTGGTTCTTTGGGGGAAAACAAATGATTATACGAAGGAAGAAGACAGAGAAAAGTGTAGAGTTGAAACACAAGAAGAGGATTGTGAATCAGCTTCAACTTCGATTGAGACAGATAGCTATAAAGTTGATGTGAGGGAACCCCTTTTGTCGTCTGGGTGTACACATCATATTGACAGCTAGGCAAACGGATCTACGACATATGCCACGTGGCATTTATGTATGCTCCACGTGTTCTCCAAGAAAAAAGGAAAAATATGTGTGAGATATATTTTTTTATTCTTGAACCTTTTTGTTTTCTTTTGTTTTAATCTTTACGAGTTTTAAGCTTAAT

Coding sequence (CDS)

ATGGGTGTGGTTGAAGAGTACTTGCCAGCAATGGCGATGTTGGGAATTCAAGCAATTTATGCCATTGTCACACTCATCTCTAGGGCTGCCCTGTTGGAGGGTATGAGTCCTAGAGTGTTCATCGTTTATAGGCAGGCCTTTGCCACTCTCTCTATTGCACCCATTGCTTATCTCTCCAGGTCGAAATCCATGAAGGTGTCTTTGGACCTCAAGAGCTTTTATCTCATCTTTTTTGCTGCACTTATCGGTTCAACCATGAACCACAATTTTTTCTACGAGGGACTGTTCTTAGCCAGTTCATCATTAGCAACCGCGATGGAGAATCTGATTCCGGCAGTGACATTTATAATAGCAGCAATGGTCGGAATGGAGAGCGTGAAGCTGACAAACTTGAGGTGCATGGCTAAGATTGGAGGTACAGTGGTTTGTGTGGGTGGGGCTATGTTCATGGCCCTTCTAAGAGGTCCAAAGCTTCTGAATGCCACACAAGCTTTTGGTGTCAAATCTGCAATATTTGGTGTGGAAAGTGGAAGCGATGAGGCTTGGTTGTTGGGGTCACTCAGTCTGTTTGGGAGCTGTTGCTGTTGGTCAATTTGGTTGATATTGCAGGTGCCGGCAATGGCAAGCTATCCTGACAAGCTATCCTTATCGGCATGGACTTGTTTTTTTTCACTCATACAATCAGTGGGTTTCACATTATGGGTAGAGGGAGCCAATGTGGAAACGTGGAAGATTCATTCAAAGACTGAACTTGTTTGTTACCTGTTCTCAGGGATTTTTGGGTCAGGAGTTGCCTACTTTCTTCAAGCTTGGGGTATTTCCAAGAGGGGACCTGTCTTCTCTGCAGTGTTCAACCCTTTTTGCACCATTATTACCACTATTTTGGCAGCTATATTTCTTCATGAGGAGATTTATACCGGAAGCTTGTTGGGGGGCGTGATTGTAATTATTGGATTATATGTGGTTCTTTGGGGGAAAACAAATGATTATACGAAGGAAGAAGACAGAGAAAAGTGTAGAGTTGAAACACAAGAAGAGGATTGTGAATCAGCTTCAACTTCGATTGAGACAGATAGCTATAAAGTTGATGTGAGGGAACCCCTTTTGTCGTCTGGGTGTACACATCATATTGACAGCTAG

Protein sequence

MGVVEEYLPAMAMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSRSKSMKVSLDLKSFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAMVGMESVKLTNLRCMAKIGGTVVCVGGAMFMALLRGPKLLNATQAFGVKSAIFGVESGSDEAWLLGSLSLFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWVEGANVETWKIHSKTELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTILAAIFLHEEIYTGSLLGGVIVIIGLYVVLWGKTNDYTKEEDREKCRVETQEEDCESASTSIETDSYKVDVREPLLSSGCTHHIDS

Homology

[Close]

BLAST of CmaCh12G008270 vs. ExPASy Swiss-Prot
Match: Q9M0B8 (WAT1-related protein At4g30420 OS=Arabidopsis thaliana OX=3702 GN=At4g30420 PE=3 SV=1)

HSP 1 Score: 339.3 bits (869), Expect = 5.4e-92
Identity = 190/366 (51.91%), Postives = 247/366 (67.49%), Query Frame = 0


Query: 11  MAMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSRSKSMKV--SL 70
           MAM  IQ  YA VTL +RA L+ G+SPRVFI+YRQAFAT+ I P  YLSR KS     SL
Sbjct: 1   MAMTMIQLCYAGVTLFARATLVHGLSPRVFILYRQAFATIFIFPFLYLSRRKSKIAISSL 60

Query: 71  DLKSFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAMVGMESVKL 130
           DLKSF LIF  +LIG T+N N + EGL+L SSS+ +A+ N+IPA+TF+I+ + G E + L
Sbjct: 61  DLKSFSLIFLVSLIGITINQNLYLEGLYLTSSSMGSAVGNIIPAITFLISFLAGYEKLNL 120

Query: 131 TNLRCMAKIGGTVVCVGGAMFMALLRGPKLLNATQAFGVKSAIFGVESGSDEAWLLGSLS 190
            ++R +AKI GT++CV GA+ M LLRGPK+LN+  A  +  ++ G        WL+G L 
Sbjct: 121 RDIRGLAKIAGTILCVAGAISMTLLRGPKILNSESALPIAKSVLG-HLKDQNTWLIGCLF 180

Query: 191 LFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWVEGANVETWKIHSK 250
           LF S  CWS WLILQVP  A YPD LSLSAW C F  IQ    T ++E  +   W +HS 
Sbjct: 181 LFSSTLCWSFWLILQVPISAYYPDNLSLSAWMCLFGTIQCAVVTFFLE-KDPNAWILHSY 240

Query: 251 TELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTILAAIFLHEEIYTGS 310
           +E    L++GI  S +++ +QAW I+KRGPVFSA+FNP CT+I TILAA+F HEEIYTGS
Sbjct: 241 SEFATCLYAGIGASALSFTVQAWAIAKRGPVFSALFNPLCTVIVTILAALFFHEEIYTGS 300

Query: 311 LLGGVIVIIGLYVVLWGKTNDYTKEEDR----EKCRVETQEEDCESASTSIETDSYKVDV 370
           L+GG+ VI+GLY VLWGK  D    +D+    +K  V+   ED  S++T+I       D+
Sbjct: 301 LIGGLGVILGLYTVLWGKAKDVMMNQDQRDNDQKSEVKIHIED--SSNTTI----CNKDL 358

[Close]

BLAST of CmaCh12G008270 vs. ExPASy Swiss-Prot
Match: Q9SUD5 (WAT1-related protein At4g28040 OS=Arabidopsis thaliana OX=3702 GN=At4g28040 PE=2 SV=1)

HSP 1 Score: 274.6 bits (701), Expect = 1.6e-72
Identity = 161/369 (43.63%), Postives = 232/369 (62.87%), Query Frame = 0


Query: 4   VEEYLPAMAMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSR-SK 63
           + +Y   +A++ +Q   A V L ++AA +EG++P VF+VYRQA ATL I PI+++S   K
Sbjct: 3   ISKYKAVLALVMLQFTSAGVALFTKAAFMEGLNPTVFVVYRQAIATLFICPISFISAWRK 62

Query: 64  SMKVSLDLKSFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAMVG 123
             K SL ++ F+ +   A+IG T+N N +++G+ L+SSS+A AM NLIPAVTFII+ +VG
Sbjct: 63  ENKPSLGVRGFWWVALTAVIGVTVNQNAYFKGIDLSSSSMACAMTNLIPAVTFIISIIVG 122

Query: 124 MESVKLTNLRCMAKIGGTVVCVGGAMFMALLRGPKLLNATQAFGVKSAIFGVESGSDEAW 183
            ES+K  +++ +AK+ GT VCVGGAM M  LRGPKLLNA            + +  + AW
Sbjct: 123 FESIKRRSMKSVAKVIGTGVCVGGAMAMTFLRGPKLLNA------------LLNQDNTAW 182

Query: 184 LLGSLSLFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWVEGANVET 243
           LLG   L  S   WS+WLILQVP  +  PD L  SA TCF + I S    L +   ++  
Sbjct: 183 LLGCFFLLISTFAWSLWLILQVPIASHCPDHLYTSACTCFIATIASFLVALALGNTHLPP 242

Query: 244 WKIHSKTELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTILAAIFLHE 303
           WK+ S  +L C ++SG F   +++FLQAW +S++GPVFSA+FNP   +I T   A++L E
Sbjct: 243 WKLDSFLKLSCCIYSG-FQLAISFFLQAWIVSQKGPVFSALFNPLSAVIVTFFGALYLKE 302

Query: 304 EIYTGSLLGGVIVIIGLYVVLWGKTNDYTKEEDREKCRVE-TQEEDCESASTSIETDSYK 363
           + Y GSLLG + +I+GLY+VLWGK+ DY +E    K   E       +  S  I   +++
Sbjct: 303 QTYLGSLLGALAIILGLYIVLWGKSEDYQEESTDLKLENEHNTSSQSDIVSIMIGDKAFR 358

Query: 364 -VDVREPLL 370
             ++ EPLL
Sbjct: 363 SSELLEPLL 358

[Close]

BLAST of CmaCh12G008270 vs. ExPASy Swiss-Prot
Match: Q94AP3 (Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1)

HSP 1 Score: 217.6 bits (553), Expect = 2.4e-55
Identity = 140/375 (37.33%), Postives = 213/375 (56.80%), Query Frame = 0


Query: 2   GVVEEYLPAMAMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSRS 61
           GV E+    +AML +Q  YA   ++SRAAL  G+S  VF VYR   A L + P AY    
Sbjct: 13  GVPEKLQLHIAMLTLQFGYAGFHVVSRAALNMGISKLVFPVYRNIIALLLLLPFAYFLEK 72

Query: 62  KSMKVSLDLKSFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAMV 121
           K  + ++ L      FF ALIG T N  F+  GL   S + A++M+N +PA+TF++AA++
Sbjct: 73  KE-RPAITLNFLIQFFFLALIGITANQGFYLLGLDNTSPTFASSMQNSVPAITFLMAALL 132

Query: 122 GMESVKLTNLRCMAKIGGTVVCVGGAMFMALLRGPKL------LNATQAFGVKSAIFGVE 181
            +E V++     ++KI GT +CV GA  + L +GP +      L+A       + +  + 
Sbjct: 133 RIEKVRINRRDGISKILGTALCVAGASVITLYKGPTIYTPASHLHAHLLTTNSAVLAPLG 192

Query: 182 SGSDEAWLLGSLSLFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWV 241
           + + + W LG + L G C  WS WL+ Q P + SYP +LS++++TCFF +IQ +    + 
Sbjct: 193 NAAPKNWTLGCIYLIGHCLSWSGWLVFQAPVLKSYPARLSVTSYTCFFGIIQFLIIAAFC 252

Query: 242 EGANVETWKIHSKTELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTIL 301
           E  + + W  HS  EL   L++GI  SG+A+ +Q W I + GPVF AV+ P  T++  I+
Sbjct: 253 E-RDSQAWVFHSGWELFTILYAGIVASGIAFAVQIWCIDRGGPVFVAVYQPVQTLVVAIM 312

Query: 302 AAIFLHEEIYTGSLLGGVIVIIGLYVVLWGKTNDYTKEEDREKCRVETQ-EEDCESASTS 361
           A+I L EE Y G ++G V++I GLY VL+GK+ +  K    EK  +++  E   E A  S
Sbjct: 313 ASIALGEEFYLGGIIGAVLIIAGLYFVLYGKSEE-RKFAALEKAAIQSSAEHGIERAPVS 372

Query: 362 IETDSYKVDVREPLL 370
              +S K  +  PLL
Sbjct: 373 --RNSIKSSITTPLL 382

[Close]

BLAST of CmaCh12G008270 vs. ExPASy Swiss-Prot
Match: Q6J163 (Auxin-induced protein 5NG4 OS=Pinus taeda OX=3352 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 1.0e-53
Identity = 138/371 (37.20%), Postives = 207/371 (55.80%), Query Frame = 0


Query: 12  AMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSRSKSMKVSLDLK 71
           AML +Q  YA   ++SRAAL  G+S  VF VYR   A + I P AY    K  + +L L 
Sbjct: 22  AMLALQFGYAGFHIVSRAALNMGVSKVVFPVYRNILALMLIGPCAYFLEKKE-RPALTLS 81

Query: 72  SFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAMVGMESVKLTNL 131
                F  AL G T         + L   + A+A++N +PA+TFI+AA + +E V ++  
Sbjct: 82  FLIQFFLLALCGITGQSRILSLRIVLHIPTFASAIQNSVPAITFIMAAALRLEKVHISRR 141

Query: 132 RCMAKIGGTVVCVGGAMFMALLRGPKLLNATQA-FGVKSAIFGVESGSD-----EAWLLG 191
             +AKI GTV CV GA  + L +GP + +  +    V ++ F    G+D     E W LG
Sbjct: 142 DGLAKIIGTVACVSGATIITLYKGPPITHIWRPNLEVTASYFKAFQGNDLSAKSENWTLG 201

Query: 192 SLSLFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWVEGANVETWKI 251
            + L G+C  WS W++LQ P +  YP +LS++++TCFF +IQ +    + E  ++E WKI
Sbjct: 202 CIYLLGNCLAWSGWIVLQAPVLKRYPARLSVTSFTCFFGVIQFLIIAAFFE-TDLEHWKI 261

Query: 252 HSKTELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTILAAIFLHEEIY 311
           HS  EL   L++G   SG+A+ +Q W I + GPVF AV+ P  TI   I+A+I L E+ Y
Sbjct: 262 HSGGELFTILYAGFVASGIAFSVQIWCIDRGGPVFVAVYQPVQTIAVAIMASIILGEQFY 321

Query: 312 TGSLLGGVIVIIGLYVVLWGKTNDYTKEEDREKCRV--ETQEEDCESASTSIETDSYKVD 371
            G + G +++IIGLY+VLWGK+ +      + K  +  E Q ++ + ++T I   S  + 
Sbjct: 322 LGGIFGAILIIIGLYLVLWGKSEEKRLGLLQAKSSMVPENQPDNMDQSATLIINSSNGI- 381

Query: 372 VREPLLSSGCT 375
             +P  SS  T
Sbjct: 382 --KPNTSSSLT 387

[Close]

BLAST of CmaCh12G008270 vs. ExPASy Swiss-Prot
Match: Q9LV20 (WAT1-related protein At3g18200 OS=Arabidopsis thaliana OX=3702 GN=At3g18200 PE=2 SV=1)

HSP 1 Score: 210.3 bits (534), Expect = 3.8e-53
Identity = 134/362 (37.02%), Postives = 206/362 (56.91%), Query Frame = 0


Query: 2   GVVEEYLP-AMAMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSR 61
           GVV E +   +A++ +Q  +A   ++SR AL  G+S  V+ VYR   A L I P AY   
Sbjct: 27  GVVSEKVKLVVALITLQFCFAGFHIVSRVALNIGVSKVVYPVYRNLLALLLIGPFAYFFE 86

Query: 62  SKSMKVSLDLKSFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAM 121
            K  +  L +      FF ALIG T N  F+  GL+ A+ + A+AM+N +PA+TFI+A  
Sbjct: 87  KKE-RPPLTISLLAQFFFLALIGITANQGFYLLGLYYATPTFASAMQNSVPAITFIMACA 146

Query: 122 VGMESVKLTNLRCMAKIGGTVVCVGGAMFMALLRGPKLLNATQAFGV-KSAIFGVESGSD 181
           + +E + L     +AK+ GT+V +GGA  + L RG  + +  Q   + K  + G  S + 
Sbjct: 147 LRLEHIDLVRKHGVAKVLGTLVSIGGATVITLYRGFPIFD--QGLNMQKEEVVG--SDNS 206

Query: 182 EAWLLGSLSLFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWVEGAN 241
            +  LG L L G C  W+ W++LQ P +  YP KL+L+++TCFF LIQ +   L+VE  +
Sbjct: 207 HSLTLGWLYLMGHCLSWAGWMVLQAPVLKQYPAKLTLTSFTCFFGLIQFLVIALFVE-TD 266

Query: 242 VETWKIHSKTELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTILAAIF 301
           +  W I S  EL   L++GI  SG+  +LQ W I K GPVF AVF P  T++   +A + 
Sbjct: 267 LNNWIIVSWEELFTILYAGIIASGLVVYLQTWCIYKSGPVFVAVFQPLQTLLVAAMAFLI 326

Query: 302 LHEEIYTGSLLGGVIVIIGLYVVLWGKTNDYTKEEDREKCRVETQEEDCESASTSIETDS 361
           L +++Y+G ++G V +++GLY+VLWGK        +  K  +E  ++D ES +  +    
Sbjct: 327 LGDQLYSGGIVGAVFIMLGLYLVLWGK-------NEERKLALEESQQDPESLTKHLLEAQ 375

[Close]

BLAST of CmaCh12G008270 vs. TAIR 10
Match: AT4G30420.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 339.3 bits (869), Expect = 3.9e-93
Identity = 190/366 (51.91%), Postives = 247/366 (67.49%), Query Frame = 0


Query: 11  MAMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSRSKSMKV--SL 70
           MAM  IQ  YA VTL +RA L+ G+SPRVFI+YRQAFAT+ I P  YLSR KS     SL
Sbjct: 1   MAMTMIQLCYAGVTLFARATLVHGLSPRVFILYRQAFATIFIFPFLYLSRRKSKIAISSL 60

Query: 71  DLKSFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAMVGMESVKL 130
           DLKSF LIF  +LIG T+N N + EGL+L SSS+ +A+ N+IPA+TF+I+ + G E + L
Sbjct: 61  DLKSFSLIFLVSLIGITINQNLYLEGLYLTSSSMGSAVGNIIPAITFLISFLAGYEKLNL 120

Query: 131 TNLRCMAKIGGTVVCVGGAMFMALLRGPKLLNATQAFGVKSAIFGVESGSDEAWLLGSLS 190
            ++R +AKI GT++CV GA+ M LLRGPK+LN+  A  +  ++ G        WL+G L 
Sbjct: 121 RDIRGLAKIAGTILCVAGAISMTLLRGPKILNSESALPIAKSVLG-HLKDQNTWLIGCLF 180

Query: 191 LFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWVEGANVETWKIHSK 250
           LF S  CWS WLILQVP  A YPD LSLSAW C F  IQ    T ++E  +   W +HS 
Sbjct: 181 LFSSTLCWSFWLILQVPISAYYPDNLSLSAWMCLFGTIQCAVVTFFLE-KDPNAWILHSY 240

Query: 251 TELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTILAAIFLHEEIYTGS 310
           +E    L++GI  S +++ +QAW I+KRGPVFSA+FNP CT+I TILAA+F HEEIYTGS
Sbjct: 241 SEFATCLYAGIGASALSFTVQAWAIAKRGPVFSALFNPLCTVIVTILAALFFHEEIYTGS 300

Query: 311 LLGGVIVIIGLYVVLWGKTNDYTKEEDR----EKCRVETQEEDCESASTSIETDSYKVDV 370
           L+GG+ VI+GLY VLWGK  D    +D+    +K  V+   ED  S++T+I       D+
Sbjct: 301 LIGGLGVILGLYTVLWGKAKDVMMNQDQRDNDQKSEVKIHIED--SSNTTI----CNKDL 358

[Close]

BLAST of CmaCh12G008270 vs. TAIR 10
Match: AT4G28040.1 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 274.6 bits (701), Expect = 1.2e-73
Identity = 161/369 (43.63%), Postives = 232/369 (62.87%), Query Frame = 0


Query: 4   VEEYLPAMAMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSR-SK 63
           + +Y   +A++ +Q   A V L ++AA +EG++P VF+VYRQA ATL I PI+++S   K
Sbjct: 3   ISKYKAVLALVMLQFTSAGVALFTKAAFMEGLNPTVFVVYRQAIATLFICPISFISAWRK 62

Query: 64  SMKVSLDLKSFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAMVG 123
             K SL ++ F+ +   A+IG T+N N +++G+ L+SSS+A AM NLIPAVTFII+ +VG
Sbjct: 63  ENKPSLGVRGFWWVALTAVIGVTVNQNAYFKGIDLSSSSMACAMTNLIPAVTFIISIIVG 122

Query: 124 MESVKLTNLRCMAKIGGTVVCVGGAMFMALLRGPKLLNATQAFGVKSAIFGVESGSDEAW 183
            ES+K  +++ +AK+ GT VCVGGAM M  LRGPKLLNA            + +  + AW
Sbjct: 123 FESIKRRSMKSVAKVIGTGVCVGGAMAMTFLRGPKLLNA------------LLNQDNTAW 182

Query: 184 LLGSLSLFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWVEGANVET 243
           LLG   L  S   WS+WLILQVP  +  PD L  SA TCF + I S    L +   ++  
Sbjct: 183 LLGCFFLLISTFAWSLWLILQVPIASHCPDHLYTSACTCFIATIASFLVALALGNTHLPP 242

Query: 244 WKIHSKTELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTILAAIFLHE 303
           WK+ S  +L C ++SG F   +++FLQAW +S++GPVFSA+FNP   +I T   A++L E
Sbjct: 243 WKLDSFLKLSCCIYSG-FQLAISFFLQAWIVSQKGPVFSALFNPLSAVIVTFFGALYLKE 302

Query: 304 EIYTGSLLGGVIVIIGLYVVLWGKTNDYTKEEDREKCRVE-TQEEDCESASTSIETDSYK 363
           + Y GSLLG + +I+GLY+VLWGK+ DY +E    K   E       +  S  I   +++
Sbjct: 303 QTYLGSLLGALAIILGLYIVLWGKSEDYQEESTDLKLENEHNTSSQSDIVSIMIGDKAFR 358

Query: 364 -VDVREPLL 370
             ++ EPLL
Sbjct: 363 SSELLEPLL 358

[Close]

BLAST of CmaCh12G008270 vs. TAIR 10
Match: AT4G28040.2 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 274.6 bits (701), Expect = 1.2e-73
Identity = 161/369 (43.63%), Postives = 232/369 (62.87%), Query Frame = 0


Query: 4   VEEYLPAMAMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSR-SK 63
           + +Y   +A++ +Q   A V L ++AA +EG++P VF+VYRQA ATL I PI+++S   K
Sbjct: 3   ISKYKAVLALVMLQFTSAGVALFTKAAFMEGLNPTVFVVYRQAIATLFICPISFISAWRK 62

Query: 64  SMKVSLDLKSFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAMVG 123
             K SL ++ F+ +   A+IG T+N N +++G+ L+SSS+A AM NLIPAVTFII+ +VG
Sbjct: 63  ENKPSLGVRGFWWVALTAVIGVTVNQNAYFKGIDLSSSSMACAMTNLIPAVTFIISIIVG 122

Query: 124 MESVKLTNLRCMAKIGGTVVCVGGAMFMALLRGPKLLNATQAFGVKSAIFGVESGSDEAW 183
            ES+K  +++ +AK+ GT VCVGGAM M  LRGPKLLNA            + +  + AW
Sbjct: 123 FESIKRRSMKSVAKVIGTGVCVGGAMAMTFLRGPKLLNA------------LLNQDNTAW 182

Query: 184 LLGSLSLFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWVEGANVET 243
           LLG   L  S   WS+WLILQVP  +  PD L  SA TCF + I S    L +   ++  
Sbjct: 183 LLGCFFLLISTFAWSLWLILQVPIASHCPDHLYTSACTCFIATIASFLVALALGNTHLPP 242

Query: 244 WKIHSKTELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTILAAIFLHE 303
           WK+ S  +L C ++SG F   +++FLQAW +S++GPVFSA+FNP   +I T   A++L E
Sbjct: 243 WKLDSFLKLSCCIYSG-FQLAISFFLQAWIVSQKGPVFSALFNPLSAVIVTFFGALYLKE 302

Query: 304 EIYTGSLLGGVIVIIGLYVVLWGKTNDYTKEEDREKCRVE-TQEEDCESASTSIETDSYK 363
           + Y GSLLG + +I+GLY+VLWGK+ DY +E    K   E       +  S  I   +++
Sbjct: 303 QTYLGSLLGALAIILGLYIVLWGKSEDYQEESTDLKLENEHNTSSQSDIVSIMIGDKAFR 358

Query: 364 -VDVREPLL 370
             ++ EPLL
Sbjct: 363 SSELLEPLL 358

[Close]

BLAST of CmaCh12G008270 vs. TAIR 10
Match: AT4G28040.3 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 274.6 bits (701), Expect = 1.2e-73
Identity = 161/369 (43.63%), Postives = 232/369 (62.87%), Query Frame = 0


Query: 4   VEEYLPAMAMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSR-SK 63
           + +Y   +A++ +Q   A V L ++AA +EG++P VF+VYRQA ATL I PI+++S   K
Sbjct: 3   ISKYKAVLALVMLQFTSAGVALFTKAAFMEGLNPTVFVVYRQAIATLFICPISFISAWRK 62

Query: 64  SMKVSLDLKSFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAMVG 123
             K SL ++ F+ +   A+IG T+N N +++G+ L+SSS+A AM NLIPAVTFII+ +VG
Sbjct: 63  ENKPSLGVRGFWWVALTAVIGVTVNQNAYFKGIDLSSSSMACAMTNLIPAVTFIISIIVG 122

Query: 124 MESVKLTNLRCMAKIGGTVVCVGGAMFMALLRGPKLLNATQAFGVKSAIFGVESGSDEAW 183
            ES+K  +++ +AK+ GT VCVGGAM M  LRGPKLLNA            + +  + AW
Sbjct: 123 FESIKRRSMKSVAKVIGTGVCVGGAMAMTFLRGPKLLNA------------LLNQDNTAW 182

Query: 184 LLGSLSLFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWVEGANVET 243
           LLG   L  S   WS+WLILQVP  +  PD L  SA TCF + I S    L +   ++  
Sbjct: 183 LLGCFFLLISTFAWSLWLILQVPIASHCPDHLYTSACTCFIATIASFLVALALGNTHLPP 242

Query: 244 WKIHSKTELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTILAAIFLHE 303
           WK+ S  +L C ++SG F   +++FLQAW +S++GPVFSA+FNP   +I T   A++L E
Sbjct: 243 WKLDSFLKLSCCIYSG-FQLAISFFLQAWIVSQKGPVFSALFNPLSAVIVTFFGALYLKE 302

Query: 304 EIYTGSLLGGVIVIIGLYVVLWGKTNDYTKEEDREKCRVE-TQEEDCESASTSIETDSYK 363
           + Y GSLLG + +I+GLY+VLWGK+ DY +E    K   E       +  S  I   +++
Sbjct: 303 QTYLGSLLGALAIILGLYIVLWGKSEDYQEESTDLKLENEHNTSSQSDIVSIMIGDKAFR 358

Query: 364 -VDVREPLL 370
             ++ EPLL
Sbjct: 363 SSELLEPLL 358

[Close]

BLAST of CmaCh12G008270 vs. TAIR 10
Match: AT4G28040.4 (nodulin MtN21 /EamA-like transporter family protein )

HSP 1 Score: 274.6 bits (701), Expect = 1.2e-73
Identity = 161/369 (43.63%), Postives = 232/369 (62.87%), Query Frame = 0


Query: 4   VEEYLPAMAMLGIQAIYAIVTLISRAALLEGMSPRVFIVYRQAFATLSIAPIAYLSR-SK 63
           + +Y   +A++ +Q   A V L ++AA +EG++P VF+VYRQA ATL I PI+++S   K
Sbjct: 3   ISKYKAVLALVMLQFTSAGVALFTKAAFMEGLNPTVFVVYRQAIATLFICPISFISAWRK 62

Query: 64  SMKVSLDLKSFYLIFFAALIGSTMNHNFFYEGLFLASSSLATAMENLIPAVTFIIAAMVG 123
             K SL ++ F+ +   A+IG T+N N +++G+ L+SSS+A AM NLIPAVTFII+ +VG
Sbjct: 63  ENKPSLGVRGFWWVALTAVIGVTVNQNAYFKGIDLSSSSMACAMTNLIPAVTFIISIIVG 122

Query: 124 MESVKLTNLRCMAKIGGTVVCVGGAMFMALLRGPKLLNATQAFGVKSAIFGVESGSDEAW 183
            ES+K  +++ +AK+ GT VCVGGAM M  LRGPKLLNA            + +  + AW
Sbjct: 123 FESIKRRSMKSVAKVIGTGVCVGGAMAMTFLRGPKLLNA------------LLNQDNTAW 182

Query: 184 LLGSLSLFGSCCCWSIWLILQVPAMASYPDKLSLSAWTCFFSLIQSVGFTLWVEGANVET 243
           LLG   L  S   WS+WLILQVP  +  PD L  SA TCF + I S    L +   ++  
Sbjct: 183 LLGCFFLLISTFAWSLWLILQVPIASHCPDHLYTSACTCFIATIASFLVALALGNTHLPP 242

Query: 244 WKIHSKTELVCYLFSGIFGSGVAYFLQAWGISKRGPVFSAVFNPFCTIITTILAAIFLHE 303
           WK+ S  +L C ++SG F   +++FLQAW +S++GPVFSA+FNP   +I T   A++L E
Sbjct: 243 WKLDSFLKLSCCIYSG-FQLAISFFLQAWIVSQKGPVFSALFNPLSAVIVTFFGALYLKE 302

Query: 304 EIYTGSLLGGVIVIIGLYVVLWGKTNDYTKEEDREKCRVE-TQEEDCESASTSIETDSYK 363
           + Y GSLLG + +I+GLY+VLWGK+ DY +E    K   E       +  S  I   +++
Sbjct: 303 QTYLGSLLGALAIILGLYIVLWGKSEDYQEESTDLKLENEHNTSSQSDIVSIMIGDKAFR 358

Query: 364 -VDVREPLL 370
             ++ EPLL
Sbjct: 363 SSELLEPLL 358

The following BLAST results are available for this feature:

BLAST of CmaCh12G008270 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9M0B8	5.4e-92	51.91	WAT1-related protein At4g30420 OS=Arabidopsis thaliana OX=3702 GN=At4g30420 PE=3...	[more]
Q9SUD5	1.6e-72	43.63	WAT1-related protein At4g28040 OS=Arabidopsis thaliana OX=3702 GN=At4g28040 PE=2...	[more]
Q94AP3	2.4e-55	37.33	Protein WALLS ARE THIN 1 OS=Arabidopsis thaliana OX=3702 GN=WAT1 PE=1 SV=1	[more]
Q6J163	1.0e-53	37.20	Auxin-induced protein 5NG4 OS=Pinus taeda OX=3352 PE=2 SV=1	[more]
Q9LV20	3.8e-53	37.02	WAT1-related protein At3g18200 OS=Arabidopsis thaliana OX=3702 GN=At3g18200 PE=2...	[more]

BLAST of CmaCh12G008270 vs. TAIR 10


Match Name	E-value	Identity	Description
AT4G30420.1	3.9e-93	51.91	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G28040.1	1.2e-73	43.63	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G28040.2	1.2e-73	43.63	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G28040.3	1.2e-73	43.63	nodulin MtN21 /EamA-like transporter family protein	[more]
AT4G28040.4	1.2e-73	43.63	nodulin MtN21 /EamA-like transporter family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000620	EamA domain	PFAM	PF00892	EamA	coord: 11..131 e-value: 3.0E-8 score: 33.9 coord: 184..323 e-value: 1.7E-16 score: 60.6
IPR030184	WAT1-related protein	PANTHER	PTHR31218	WAT1-RELATED PROTEIN	coord: 10..347
None	No IPR available	PANTHER	PTHR31218:SF244	WAT1-RELATED PROTEIN	coord: 10..347
None	No IPR available	SUPERFAMILY	103481	Multidrug resistance efflux transporter EmrE	coord: 220..326

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G008270.1	CmaCh12G008270.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0055085	transmembrane transport
cellular_component	GO:0016021	integral component of membrane
cellular_component	GO:0016020	membrane
molecular_function	GO:0022857	transmembrane transporter activity