CmaCh12G007470 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh12G007470 vs. ExPASy Swiss-Prot
Match: Q8L9J9 (Probable carbohydrate esterase At4g34215 OS=Arabidopsis thaliana OX=3702 GN=At4g34215 PE=1 SV=2)

HSP 1 Score: 180.3 bits (456), Expect = 3.1e-44
Identity = 98/271 (36.16%), Postives = 151/271 (55.72%), Query Frame = 0

Query: 8   PSLSGATSPTNIFILAGQSNMAGRGGVEKT-PKGELVWDGKVPSECQSDPSILRFNPERQ 67
           P +     P  IFIL+GQSNMAGRGGV K       VWD  +P EC  + SILR + + +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 68  WEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIK 127
           WE AHEPLH+ ID G  K  G+GPG+ FA+ +K +    + ++GLVPCA GGT I++W +
Sbjct: 73  WEEAHEPLHVDIDTG--KVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER 132

Query: 128 NPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIR 187
                 +  Y+  ++R + S K GG ++A+ WYQGESD      A+ Y +N+ + I ++R
Sbjct: 133 -----GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLR 192

Query: 188 NDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMNLTT 247
           +D+    LP+I V IA    ++          +  E  +  +L +++ +D+  LP+    
Sbjct: 193 HDLNLPSLPIIQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGLPLKS-- 252

Query: 248 FEGFSWDHGHFNTATEIALGKWLADTYLAHY 278
                 D+ H  T  ++ LG  LA  YL+++
Sbjct: 253 ------DNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of CmaCh12G007470 vs. ExPASy TrEMBL
Match: A0A6J1I774 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111471873 PE=4 SV=1)

HSP 1 Score: 585.5 bits (1508), Expect = 1.2e-163
Identity = 280/281 (99.64%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 1   MCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPKGELVWDGKVPSECQSDPSILR 60
           MCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTP GELVWDGKVPSECQSDPSILR
Sbjct: 10  MCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTGELVWDGKVPSECQSDPSILR 69

Query: 61  FNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTL 120
           FNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTL
Sbjct: 70  FNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTL 129

Query: 121 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 180
           IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK
Sbjct: 130 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 189

Query: 181 FITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL 240
           FITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL
Sbjct: 190 FITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL 249

Query: 241 PMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 282
           PMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL
Sbjct: 250 PMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

BLAST of CmaCh12G007470 vs. ExPASy TrEMBL
Match: A0A6J1IKT8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111474939 PE=4 SV=1)

HSP 1 Score: 538.1 bits (1385), Expect = 2.2e-149
Identity = 254/254 (100.00%), Postives = 254/254 (100.00%), Query Frame = 0

Query: 28  MAGRGGVEKTPKGELVWDGKVPSECQSDPSILRFNPERQWEIAHEPLHLGIDVGKTKTPG 87
           MAGRGGVEKTPKGELVWDGKVPSECQSDPSILRFNPERQWEIAHEPLHLGIDVGKTKTPG
Sbjct: 1   MAGRGGVEKTPKGELVWDGKVPSECQSDPSILRFNPERQWEIAHEPLHLGIDVGKTKTPG 60

Query: 88  IGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSE 147
           IGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSE
Sbjct: 61  IGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIKNPSNPSATFYQNFIERIKTSE 120

Query: 148 KEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPVIIVKIALYDFF 207
           KEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPVIIVKIALYDFF
Sbjct: 121 KEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIRNDIKPRFLPVIIVKIALYDFF 180

Query: 208 MKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMNLTTFEGFSWDHGHFNTATEIALGK 267
           MKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMNLTTFEGFSWDHGHFNTATEIALGK
Sbjct: 181 MKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMNLTTFEGFSWDHGHFNTATEIALGK 240

Query: 268 WLADTYLAHYGHLL 282
           WLADTYLAHYGHLL
Sbjct: 241 WLADTYLAHYGHLL 254

BLAST of CmaCh12G007470 vs. ExPASy TrEMBL
Match: A0A6J1GJF6 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454406 PE=4 SV=1)

HSP 1 Score: 535.8 bits (1379), Expect = 1.1e-148
Identity = 257/281 (91.46%), Postives = 265/281 (94.31%), Query Frame = 0

Query: 1   MCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPKGELVWDGKVPSECQSDPSILR 60
           +C IL +PSLSGATSPTNIFILAGQSNMAGRGGVEK   G+LVWDGKVP ECQ DPSILR
Sbjct: 10  LCMILFNPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQFDPSILR 69

Query: 61  FNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTL 120
            NPERQWEIAHEPLHLGID+  + TPGIGPGIPFAHQ KEKAGQKAGIVGLVPCARGGTL
Sbjct: 70  LNPERQWEIAHEPLHLGIDI--SHTPGIGPGIPFAHQFKEKAGQKAGIVGLVPCARGGTL 129

Query: 121 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 180
           IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK
Sbjct: 130 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 189

Query: 181 FITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL 240
           FITDIRNDIKPRFLPVIIVKI+LYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDS EL
Sbjct: 190 FITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSREL 249

Query: 241 PMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 282
           P+N TTFEGFSWDHGHFNT TEI LGKWLA+TYLAHYGHLL
Sbjct: 250 PVNFTTFEGFSWDHGHFNTETEIVLGKWLANTYLAHYGHLL 288

BLAST of CmaCh12G007470 vs. ExPASy TrEMBL
Match: A0A6J1GIT2 (probable carbohydrate esterase At4g34215 OS=Cucurbita moschata OX=3662 GN=LOC111454649 PE=4 SV=1)

HSP 1 Score: 532.3 bits (1370), Expect = 1.2e-147
Identity = 256/281 (91.10%), Postives = 264/281 (93.95%), Query Frame = 0

Query: 1   MCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPKGELVWDGKVPSECQSDPSILR 60
           +C IL SPSLSGATSPTNIFILAGQSNMAGRGGVEK   G+LVWDGKVP EC S+PSILR
Sbjct: 10  LCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECLSNPSILR 69

Query: 61  FNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTL 120
            NPERQWEIAHEPLHLGID+  + TPGIGPGIPFAHQ KEKAGQKAGIVGLVPCARGGTL
Sbjct: 70  LNPERQWEIAHEPLHLGIDI--SHTPGIGPGIPFAHQFKEKAGQKAGIVGLVPCARGGTL 129

Query: 121 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 180
           IEQW+KNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK
Sbjct: 130 IEQWMKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 189

Query: 181 FITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL 240
           FITDIRNDIKPRFLPVIIVKI+LYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDS EL
Sbjct: 190 FITDIRNDIKPRFLPVIIVKISLYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSREL 249

Query: 241 PMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 282
           P+N TTFEGFSWD GHFNT TEI LGKWLADTYLAHYGHLL
Sbjct: 250 PVNFTTFEGFSWDRGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of CmaCh12G007470 vs. ExPASy TrEMBL
Match: A0A6J1KIR8 (probable carbohydrate esterase At4g34215 OS=Cucurbita maxima OX=3661 GN=LOC111496116 PE=4 SV=1)

HSP 1 Score: 523.9 bits (1348), Expect = 4.3e-145
Identity = 252/281 (89.68%), Postives = 260/281 (92.53%), Query Frame = 0

Query: 1   MCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPKGELVWDGKVPSECQSDPSILR 60
           +C IL  PSLS ATSPTNIFILAGQSNMAGRGGVE   KG+L WDGKVP ECQSDPSILR
Sbjct: 10  LCMILFHPSLSWATSPTNIFILAGQSNMAGRGGVENNQKGKLEWDGKVPLECQSDPSILR 69

Query: 61  FNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTL 120
            NP RQWEIA EPLHLGID+G  KTPGIGPGIPFAHQ K KAGQKAGIVGLVPCARGGTL
Sbjct: 70  LNPARQWEIAQEPLHLGIDIG--KTPGIGPGIPFAHQFKAKAGQKAGIVGLVPCARGGTL 129

Query: 121 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 180
           IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAM+DTA RYKDNLKK
Sbjct: 130 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMSDTAHRYKDNLKK 189

Query: 181 FITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL 240
           FITDIRNDIKPRFLPVIIVKI++YDFFMKHDTH+LPAVRAAEDAVQKELPDIITIDSWEL
Sbjct: 190 FITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHDLPAVRAAEDAVQKELPDIITIDSWEL 249

Query: 241 PMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 282
           P+N TTFEGF  DHGHFNTATEIALGKWLADTYLAHY HLL
Sbjct: 250 PINFTTFEGFCLDHGHFNTATEIALGKWLADTYLAHYSHLL 288

BLAST of CmaCh12G007470 vs. NCBI nr
Match: XP_022973316.1 (probable carbohydrate esterase At4g34215 [Cucurbita maxima] >XP_022973317.1 probable carbohydrate esterase At4g34215 [Cucurbita maxima])

HSP 1 Score: 585.5 bits (1508), Expect = 2.5e-163
Identity = 280/281 (99.64%), Postives = 280/281 (99.64%), Query Frame = 0

Query: 1   MCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPKGELVWDGKVPSECQSDPSILR 60
           MCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTP GELVWDGKVPSECQSDPSILR
Sbjct: 10  MCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPTGELVWDGKVPSECQSDPSILR 69

Query: 61  FNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTL 120
           FNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTL
Sbjct: 70  FNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTL 129

Query: 121 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 180
           IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK
Sbjct: 130 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 189

Query: 181 FITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL 240
           FITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL
Sbjct: 190 FITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL 249

Query: 241 PMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 282
           PMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL
Sbjct: 250 PMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 290

BLAST of CmaCh12G007470 vs. NCBI nr
Match: XP_023536885.1 (probable carbohydrate esterase At4g34215 isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536886.1 probable carbohydrate esterase At4g34215 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 545.4 bits (1404), Expect = 2.8e-151
Identity = 260/281 (92.53%), Postives = 267/281 (95.02%), Query Frame = 0

Query: 1   MCTILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPKGELVWDGKVPSECQSDPSILR 60
           +C IL SPSLSGATSPTNIFILAGQSNMAGRGGVEK   G+LVWDGKVP ECQSDPSILR
Sbjct: 10  LCMILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKIQNGKLVWDGKVPLECQSDPSILR 69

Query: 61  FNPERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTL 120
            NPERQWEIAHEPLHLGID+  + TPGIGPGIPFAHQ KEKAGQKAG VGLVPCARGGTL
Sbjct: 70  LNPERQWEIAHEPLHLGIDI--SHTPGIGPGIPFAHQFKEKAGQKAGTVGLVPCARGGTL 129

Query: 121 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 180
           IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK
Sbjct: 130 IEQWIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKK 189

Query: 181 FITDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL 240
           FITDIRNDIKPRFLPVIIVKI++YDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL
Sbjct: 190 FITDIRNDIKPRFLPVIIVKISMYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWEL 249

Query: 241 PMNLTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 282
           P+NLTTFEGFSWDHGHFNT TEI LGKWLADTYLAHYGHLL
Sbjct: 250 PINLTTFEGFSWDHGHFNTETEIVLGKWLADTYLAHYGHLL 288

BLAST of CmaCh12G007470 vs. NCBI nr
Match: KAG6585850.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 542.3 bits (1396), Expect = 2.4e-150
Identity = 263/278 (94.60%), Postives = 266/278 (95.68%), Query Frame = 0

Query: 4   ILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPKGELVWDGKVPSECQSDPSILRFNP 63
           IL SPSLSGATSPTNIFILAGQSNMAGRGGVEKT  G+LVWDGKVP ECQSDPSILR NP
Sbjct: 2   ILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKTQDGKLVWDGKVPLECQSDPSILRLNP 61

Query: 64  ERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQ 123
           ERQWEIAHEPLHLGID+G   TPGIG GIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQ
Sbjct: 62  ERQWEIAHEPLHLGIDIG--HTPGIGSGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQ 121

Query: 124 WIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFIT 183
           WIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFIT
Sbjct: 122 WIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFIT 181

Query: 184 DIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMN 243
           DIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVR AEDAVQKELPDIITIDS ELP+N
Sbjct: 182 DIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVREAEDAVQKELPDIITIDSLELPIN 241

Query: 244 LTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 282
           LTTFEGFSWDHGH NTATEIALGKWLADTYLAHYGHLL
Sbjct: 242 LTTFEGFSWDHGHLNTATEIALGKWLADTYLAHYGHLL 277

BLAST of CmaCh12G007470 vs. NCBI nr
Match: KAG6585835.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 541.2 bits (1393), Expect = 5.3e-150
Identity = 261/278 (93.88%), Postives = 264/278 (94.96%), Query Frame = 0

Query: 4   ILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPKGELVWDGKVPSECQSDPSILRFNP 63
           IL SPSLSGATSPTNIFILAGQSNMAGRGGVEK   GELVWDGKVP ECQSDPSILR NP
Sbjct: 2   ILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKNQNGELVWDGKVPLECQSDPSILRLNP 61

Query: 64  ERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQ 123
           E QWEIAHEPLHLGID+G   TPGIG GIPFAHQLKEKAGQKAG VGLVPCARGGTLIEQ
Sbjct: 62  ECQWEIAHEPLHLGIDIG--HTPGIGLGIPFAHQLKEKAGQKAGTVGLVPCARGGTLIEQ 121

Query: 124 WIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFIT 183
           WIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFIT
Sbjct: 122 WIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFIT 181

Query: 184 DIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMN 243
           DIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVR AEDAVQKELPD+ITIDSWELP+N
Sbjct: 182 DIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVREAEDAVQKELPDVITIDSWELPIN 241

Query: 244 LTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 282
           LTTFEGFSWDHGH NTATEIALGKWLADTYLAHYGHLL
Sbjct: 242 LTTFEGFSWDHGHLNTATEIALGKWLADTYLAHYGHLL 277

BLAST of CmaCh12G007470 vs. NCBI nr
Match: KAG6585826.1 (putative carbohydrate esterase, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 541.2 bits (1393), Expect = 5.3e-150
Identity = 262/278 (94.24%), Postives = 266/278 (95.68%), Query Frame = 0

Query: 4   ILLSPSLSGATSPTNIFILAGQSNMAGRGGVEKTPKGELVWDGKVPSECQSDPSILRFNP 63
           IL SPSLSGATSPTNIFILAGQSNMAGRGGVEKT  G+LVWDGKVP ECQSDPSILR NP
Sbjct: 2   ILFSPSLSGATSPTNIFILAGQSNMAGRGGVEKTQNGKLVWDGKVPLECQSDPSILRLNP 61

Query: 64  ERQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQ 123
           ERQWEIAHEPLHLGID+G   TPGIG GIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQ
Sbjct: 62  ERQWEIAHEPLHLGIDIG--HTPGIGSGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQ 121

Query: 124 WIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFIT 183
           WIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYK+NLKKFIT
Sbjct: 122 WIKNPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKENLKKFIT 181

Query: 184 DIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMN 243
           DIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVR AEDAVQKELPDIITIDS ELP+N
Sbjct: 182 DIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVREAEDAVQKELPDIITIDSLELPIN 241

Query: 244 LTTFEGFSWDHGHFNTATEIALGKWLADTYLAHYGHLL 282
           LTTFEGFSWDHGH NTATEIALGKWLADTYLAHYGHLL
Sbjct: 242 LTTFEGFSWDHGHLNTATEIALGKWLADTYLAHYGHLL 277

BLAST of CmaCh12G007470 vs. TAIR 10
Match: AT3G53010.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 182.2 bits (461), Expect = 5.8e-46
Identity = 111/273 (40.66%), Postives = 155/273 (56.78%), Query Frame = 0

Query: 8   PSLSGATSPTN--IFILAGQSNMAGRGGV-EKTPKGELVWDGKVPSECQSDPSILRFNPE 67
           P L   T   N  IFILAGQSNMAGRGGV   T     VWDG +P EC+S+PSILR   +
Sbjct: 18  PHLQSQTITRNISIFILAGQSNMAGRGGVYNDTATNTTVWDGVIPPECRSNPSILRLTSK 77

Query: 68  RQWEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQW 127
            +W+ A EPLH+ ID+ KT   G+GPG+PFA+++  + GQ    VGLVPC+ GGT + QW
Sbjct: 78  LEWKEAKEPLHVDIDINKTN--GVGPGMPFANRVVNRFGQ----VGLVPCSIGGTKLSQW 137

Query: 128 IKNPSNPSATFYQNFIERIKTSEKE--GGVVRALFWYQGESDAAMNDTAQRYKDNLKKFI 187
            K         Y+  ++R K +     GG  RA+ WYQGESD      A  YK  L KF 
Sbjct: 138 QK-----GEFLYEETVKRAKAAMASGGGGSYRAVLWYQGESDTVDMVDASVYKKRLVKFF 197

Query: 188 TDIRNDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPM 247
           +D+RND++   LP+I V +A            L AVR A+  ++ +L ++  +D+  LP+
Sbjct: 198 SDLRNDLQHPNLPIIQVALA------TGAGPYLDAVRKAQ--LKTDLENVYCVDARGLPL 257

Query: 248 NLTTFEGFSWDHGHFNTATEIALGKWLADTYLA 276
                     D  H  T++++ LG  +A+++LA
Sbjct: 258 EP--------DGLHLTTSSQVQLGHMIAESFLA 263

BLAST of CmaCh12G007470 vs. TAIR 10
Match: AT4G34215.1 (Domain of unknown function (DUF303) )

HSP 1 Score: 180.3 bits (456), Expect = 2.2e-45
Identity = 98/271 (36.16%), Postives = 151/271 (55.72%), Query Frame = 0

Query: 8   PSLSGATSPTNIFILAGQSNMAGRGGVEKT-PKGELVWDGKVPSECQSDPSILRFNPERQ 67
           P +     P  IFIL+GQSNMAGRGGV K       VWD  +P EC  + SILR + + +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 68  WEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIK 127
           WE AHEPLH+ ID G  K  G+GPG+ FA+ +K +    + ++GLVPCA GGT I++W +
Sbjct: 73  WEEAHEPLHVDIDTG--KVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER 132

Query: 128 NPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIR 187
                 +  Y+  ++R + S K GG ++A+ WYQGESD      A+ Y +N+ + I ++R
Sbjct: 133 -----GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLR 192

Query: 188 NDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMNLTT 247
           +D+    LP+I V IA    ++          +  E  +  +L +++ +D+  LP+    
Sbjct: 193 HDLNLPSLPIIQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGLPLKS-- 252

Query: 248 FEGFSWDHGHFNTATEIALGKWLADTYLAHY 278
                 D+ H  T  ++ LG  LA  YL+++
Sbjct: 253 ------DNLHLTTEAQVQLGLSLAQAYLSNF 259

BLAST of CmaCh12G007470 vs. TAIR 10
Match: AT4G34215.2 (Domain of unknown function (DUF303) )

HSP 1 Score: 180.3 bits (456), Expect = 2.2e-45
Identity = 98/271 (36.16%), Postives = 151/271 (55.72%), Query Frame = 0

Query: 8   PSLSGATSPTNIFILAGQSNMAGRGGVEKT-PKGELVWDGKVPSECQSDPSILRFNPERQ 67
           P +     P  IFIL+GQSNMAGRGGV K       VWD  +P EC  + SILR + + +
Sbjct: 13  PEIQSPIPPNQIFILSGQSNMAGRGGVFKDHHNNRWVWDKILPPECAPNSSILRLSADLR 72

Query: 68  WEIAHEPLHLGIDVGKTKTPGIGPGIPFAHQLKEKAGQKAGIVGLVPCARGGTLIEQWIK 127
           WE AHEPLH+ ID G  K  G+GPG+ FA+ +K +    + ++GLVPCA GGT I++W +
Sbjct: 73  WEEAHEPLHVDIDTG--KVCGVGPGMAFANAVKNRLETDSAVIGLVPCASGGTAIKEWER 132

Query: 128 NPSNPSATFYQNFIERIKTSEKEGGVVRALFWYQGESDAAMNDTAQRYKDNLKKFITDIR 187
                 +  Y+  ++R + S K GG ++A+ WYQGESD      A+ Y +N+ + I ++R
Sbjct: 133 -----GSHLYERMVKRTEESRKCGGEIKAVLWYQGESDVLDIHDAESYGNNMDRLIKNLR 192

Query: 188 NDIKPRFLPVIIVKIALYDFFMKHDTHNLPAVRAAEDAVQKELPDIITIDSWELPMNLTT 247
           +D+    LP+I V IA    ++          +  E  +  +L +++ +D+  LP+    
Sbjct: 193 HDLNLPSLPIIQVAIASGGGYID---------KVREAQLGLKLSNVVCVDAKGLPLKS-- 252

Query: 248 FEGFSWDHGHFNTATEIALGKWLADTYLAHY 278
                 D+ H  T  ++ LG  LA  YL+++
Sbjct: 253 ------DNLHLTTEAQVQLGLSLAQAYLSNF 259