CmaCh12G007290 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G007290
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic
LocationCma_Chr12: 4342835 .. 4346650 (+)
RNA-Seq ExpressionCmaCh12G007290
SyntenyCmaCh12G007290
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATCCCACGTCCCCATAATTTTCCACTTTTCCCTATCCCCTTCTCCAATGGGTTACTGAAGCTTCAAATCCCCGAAAATGGCGACTCCGAATCCAACTTCCATGGCGTTATCTTCTTCCTCCTCCAATTTCCTTTCCCTTCCCCGCACTGCAACATGCATTTCATTCCCACTGAAGCGCCCGCTTCTCCATCCCAAGCCGCGCCTCTCTCTAAATTCCCTCGCCTCTCCGGATTTAGACCCCTCCTTGCCCTCAAACGTTCACACCTTCTGGGAGTGGCTTCGCCAGGAGGGTCTGGTCTCCTCCAAGACTCACGTGAAGCCAGCGATTTTCCCCGAAGGCCTCGGCCTGGCCACCACCAAAGACCTATCCAAGAACGAGGTCGTTTTGGAGGTTCCCAAGCGGTTCTGGATCAACCCCGACGCGGTTGCTAATTCTGAAATTGGCAATGTCTGCAGTGGATTGAAGCCGTGGATTTCTGTTGCTCTGTTTCTGATTAGAGAGAATTTCAAGGAGGATTCGCGTTGGCGACGGTACCTAGATATTCTCCCTCAGGAAACAGATTCCACTGTGTTTTGGTGCGTTTTTGGCTTCCTTCTTACTGTTTAATCTGTGCTCCTTGTTGTGATGTGTTTTCTTCATTGTGGAGTTGATGGAGTGCTTGTCTTGATGCTTTCAGTGCAGGTCTGAAGAGGAGCTTGCGGAGCTCCAAGGTTCGTGTGTCTTATATTTGCAATTTTGGTCTCTCTATTTCCCAACCCAAATTGGTTGTAGCTAGAGCACCGAAATTAATACAAAACTTGAATTTGTTTGGGAGGAGAATGAAACACCCTTTAGGAGGCTGAGTGCCGAAGGGAGTAGACATGAGGTGATGTGCCAGCAAGAACGCTGGGCCTCGAAGAAGGTGGATTTGGTGGGGTCCTACATCAATGGTAGAAAGGATGAGTGCCAGCAGGGCCTCAAAGGAGGGTGAATTATGAGATCCCACATCGGTTGAGGAGGAGAACGAAACATCCTTACGAAGGGTGTGGAAACCTCTTCCTACGTTTTAAAAACCTTGAGGGGGCTTGGAAGGGAAAGCCCAAAGAGGACCATATCTACTAGCGGTGGGCTTGGACAAGGGTTAACTCAAGGTAGTAATGTGTATGCAGTACTTGTGTGCTAAATTCTTATGAAAAAATTAGGATTAGGAAGGAGAAATGTGTTACTGGTCGTTAGATCAGTTTGGTGTTTTCATCATGAAGATCACTCCTAACTCTTTATAATTATCCGTATATAGTTGCTTACCACATTCTTGCTTCGCGAACATTTCAGGAACCCAACTTCTGAGTACGACTTTGAATGTAAAAGAATATGTTAAGAGCGAGTTTCATAAGGTTGAAGAGATAATACTCCGCCACAAAGATCTATTTCCTACTCCAATTACACTGGATGACTTCTTTTGGGCTTTTGGGATACTCAGATCAAGAGCATTTTCACGACTCAGAGGCCAAAACCTTGTTTTGATCCCTTTCGCGGACTTGGTTAGATCTGTACTCTTACTTGATTAAGTTATTATTGTCATTGAAATTTTATGCATACAATACAAGCAGCGCCGGCGCGAAGTTGCTTCGACCCGCATTAAACGCCGGGCCGCTTGTTGAAGATAAAAGTACATTGAACCCATATGAAGTTTTACCATGAGCAGATTGAATCCATTGGACAAAAATGGGTTCGGTTAAGATTTGTTTCTCCGGAGTACCAAAAGCAATCATGACATCATTATGAACATAAAGCCCCAAAGTATGGAACCCCAGAAAAAGGCTGGCCCAACTTAAATGGGATATGATAGCTTCTTTATGGTCTAACATTCTTGCCAATACATTATCTTCATTCTGTTCTGGATTGTACTCTCTAATAAAAAATATAGCTCCATGAGCCAAAGCTCTTGTCATAATGAATCCTGCAATGTATTGGTGATGGGTATGGTTTAAAGGTTGAGTCGATCCATAAATTTCATACTTAAATTGTTTTGAAAGTTTAGGATCACTGAGCATTAAAGTTTAGAGACCATAAGAAGATTTAAATCAACAAATTGTTACATCTAAACCTTGTATCATGGATTAGTTTTATCTTTCCTATCTTTTACAATAACATTTACACATAATGAATCATTGTACAAAAGAACTATTTTTTATTCTTGTCTTCAGACAAAATCTAACTCCCGTCAAACACATTTCATATTTGATCAGGTCAACCATAGTGCTGATGTAACCTCAGAAGAACATGCATGGGAGGTTAAGGGACCAGCAGGTCTCTTCTCATGGGATATCCTATTCTCTCTCCGGTCCCCAATTTCTGTGAAGGCTGGCGAGCAGGTGAAGAACTAATTTTTATATTCATGTACCTGGGTGACATAACTGATCACATAGTTGGAGTAGAATGTTCTTGTTCGCTTGTTGAACGTTTTATTCTTAAACTGGGATTTACTTGCAGCAGTGAATAAAACGTGTGTTAGGTGTAAGATATTTAGATTAAGGGGCATTTCATTTTGATCTTCTGATAAGTAAATTGATGGTTAATTTGCATTTTACTCTAAATTCTAAATATGGGATAGTGGAAGTTTTTAGGGATCAAGTAGAAAGTAAGATTAAAACATAAAGGACTTCAAAAGGATGTGAAATGAATTTCAGAAGGGCACGACGTGGACAGGAAGTGTCAATCTATCTTGGGTTAAAGCCATTTCTCGTAGGTACCGCAGCTTTCTAGTTTCCAAACATAAAGAACCAAAAATGTAATTTGGCCTATACTCTATGCCGGAAGTGTTAGAAATAGAGAGATTAATGATATTTTGAAAGTGCATTTACCTGGAAATTTTTACTGTATGTTCAACAGGTTTTTATCCAATATGACTTGAAAAAGAGCAATGCCGACCTAGCTCTTGACTATGGGTTCGTAGAGTCAAAACCTGACCGCAATGCATATACACTGACGTTAGAGATCCCAGAGTCGGACCCTTTTTATGATGACAAGCTGGATATTGCAGAGACTAATGGTCTTGGTGAGACTGCATATTTTGACATTGTTTTGGACCGTCCTTTTCCTGATGCAATGCTTCCATTCCTGCGGTTGCTAGCGCTTGGAGGCACCGATGCATTCCTCTTGGAATCCTTATTTAGAAACTCAATCTGGGGCCATCTTGAAATGCCTGTAAGTCGAGCAAATGAAGAGTTTGTGTGTCAAGTGGTCAGAAATGCTTGTGAGGTTGCTCTTTCTGGTTACCGCACCACTTTAGAAGAGGTAGACCGTCAAATCTTCATTCATCTATTATGCATTTATCATACATTCATGTTGAATATTTACTCATTGCAATACATGAACTCATTCCCGTTTGCCATATCCATGAATTATTTTAAGCATCTTCAAGTAATTTGTGTGCTCCGAGATGTTTATTATGATCCGAATCTTATAGAATCCGATACTTTACCCTGGCTTTCGATCTCGCATCTCCTAATGATTTCTTTAGAAATCAATTTTTTAGTCCTACCATGTTATAAGTTTTAATTTCTAAACTCCAGTACTTGGTCTCTATATAAACCTCATTGGACAAGGATGATGAATTGCAGGATGAGAAGCTAAAAGAAGAAAAGCTTGATTCAAGACTGAGGATTGCTGTTGGAATAAGAGAAGGGGAGAAAAGGGTATTGCAGCAAATAATCAAAATTTTCAAAGACAGGGAACTGGAATTAGATGAATTGGAATATTACCAAGAAAGGAGGCTAAAGGATCTTGGGCTATGTGGGGAGCAAGGTGAAATTATCTTTTGGGAGTCCAAATAG

mRNA sequence

ATCCCACGTCCCCATAATTTTCCACTTTTCCCTATCCCCTTCTCCAATGGGTTACTGAAGCTTCAAATCCCCGAAAATGGCGACTCCGAATCCAACTTCCATGGCGTTATCTTCTTCCTCCTCCAATTTCCTTTCCCTTCCCCGCACTGCAACATGCATTTCATTCCCACTGAAGCGCCCGCTTCTCCATCCCAAGCCGCGCCTCTCTCTAAATTCCCTCGCCTCTCCGGATTTAGACCCCTCCTTGCCCTCAAACGTTCACACCTTCTGGGAGTGGCTTCGCCAGGAGGGTCTGGTCTCCTCCAAGACTCACGTGAAGCCAGCGATTTTCCCCGAAGGCCTCGGCCTGGCCACCACCAAAGACCTATCCAAGAACGAGGTCGTTTTGGAGGTTCCCAAGCGGTTCTGGATCAACCCCGACGCGGTTGCTAATTCTGAAATTGGCAATGTCTGCAGTGGATTGAAGCCGTGGATTTCTGTTGCTCTGTTTCTGATTAGAGAGAATTTCAAGGAGGATTCGCGTTGGCGACGGTACCTAGATATTCTCCCTCAGGAAACAGATTCCACTGTGTTTTGGTCTGAAGAGGAGCTTGCGGAGCTCCAAGGAACCCAACTTCTGAGTACGACTTTGAATGTAAAAGAATATGTTAAGAGCGAGTTTCATAAGGTTGAAGAGATAATACTCCGCCACAAAGATCTATTTCCTACTCCAATTACACTGGATGACTTCTTTTGGGCTTTTGGGATACTCAGATCAAGAGCATTTTCACGACTCAGAGGCCAAAACCTTGTTTTGATCCCTTTCGCGGACTTGGTCAACCATAGTGCTGATGTAACCTCAGAAGAACATGCATGGGAGGTTAAGGGACCAGCAGGTCTCTTCTCATGGGATATCCTATTCTCTCTCCGGTCCCCAATTTCTGTGAAGGCTGGCGAGCAGGTTTTTATCCAATATGACTTGAAAAAGAGCAATGCCGACCTAGCTCTTGACTATGGGTTCGTAGAGTCAAAACCTGACCGCAATGCATATACACTGACGTTAGAGATCCCAGAGTCGGACCCTTTTTATGATGACAAGCTGGATATTGCAGAGACTAATGGTCTTGGTGAGACTGCATATTTTGACATTGTTTTGGACCGTCCTTTTCCTGATGCAATGCTTCCATTCCTGCGGTTGCTAGCGCTTGGAGGCACCGATGCATTCCTCTTGGAATCCTTATTTAGAAACTCAATCTGGGGCCATCTTGAAATGCCTGTAAGTCGAGCAAATGAAGAGTTTGTGTGTCAAGTGGTCAGAAATGCTTGTGAGGTTGCTCTTTCTGGTTACCGCACCACTTTAGAAGAGGATGAGAAGCTAAAAGAAGAAAAGCTTGATTCAAGACTGAGGATTGCTGTTGGAATAAGAGAAGGGGAGAAAAGGGTATTGCAGCAAATAATCAAAATTTTCAAAGACAGGGAACTGGAATTAGATGAATTGGAATATTACCAAGAAAGGAGGCTAAAGGATCTTGGGCTATGTGGGGAGCAAGGTGAAATTATCTTTTGGGAGTCCAAATAG

Coding sequence (CDS)

ATGGCGACTCCGAATCCAACTTCCATGGCGTTATCTTCTTCCTCCTCCAATTTCCTTTCCCTTCCCCGCACTGCAACATGCATTTCATTCCCACTGAAGCGCCCGCTTCTCCATCCCAAGCCGCGCCTCTCTCTAAATTCCCTCGCCTCTCCGGATTTAGACCCCTCCTTGCCCTCAAACGTTCACACCTTCTGGGAGTGGCTTCGCCAGGAGGGTCTGGTCTCCTCCAAGACTCACGTGAAGCCAGCGATTTTCCCCGAAGGCCTCGGCCTGGCCACCACCAAAGACCTATCCAAGAACGAGGTCGTTTTGGAGGTTCCCAAGCGGTTCTGGATCAACCCCGACGCGGTTGCTAATTCTGAAATTGGCAATGTCTGCAGTGGATTGAAGCCGTGGATTTCTGTTGCTCTGTTTCTGATTAGAGAGAATTTCAAGGAGGATTCGCGTTGGCGACGGTACCTAGATATTCTCCCTCAGGAAACAGATTCCACTGTGTTTTGGTCTGAAGAGGAGCTTGCGGAGCTCCAAGGAACCCAACTTCTGAGTACGACTTTGAATGTAAAAGAATATGTTAAGAGCGAGTTTCATAAGGTTGAAGAGATAATACTCCGCCACAAAGATCTATTTCCTACTCCAATTACACTGGATGACTTCTTTTGGGCTTTTGGGATACTCAGATCAAGAGCATTTTCACGACTCAGAGGCCAAAACCTTGTTTTGATCCCTTTCGCGGACTTGGTCAACCATAGTGCTGATGTAACCTCAGAAGAACATGCATGGGAGGTTAAGGGACCAGCAGGTCTCTTCTCATGGGATATCCTATTCTCTCTCCGGTCCCCAATTTCTGTGAAGGCTGGCGAGCAGGTTTTTATCCAATATGACTTGAAAAAGAGCAATGCCGACCTAGCTCTTGACTATGGGTTCGTAGAGTCAAAACCTGACCGCAATGCATATACACTGACGTTAGAGATCCCAGAGTCGGACCCTTTTTATGATGACAAGCTGGATATTGCAGAGACTAATGGTCTTGGTGAGACTGCATATTTTGACATTGTTTTGGACCGTCCTTTTCCTGATGCAATGCTTCCATTCCTGCGGTTGCTAGCGCTTGGAGGCACCGATGCATTCCTCTTGGAATCCTTATTTAGAAACTCAATCTGGGGCCATCTTGAAATGCCTGTAAGTCGAGCAAATGAAGAGTTTGTGTGTCAAGTGGTCAGAAATGCTTGTGAGGTTGCTCTTTCTGGTTACCGCACCACTTTAGAAGAGGATGAGAAGCTAAAAGAAGAAAAGCTTGATTCAAGACTGAGGATTGCTGTTGGAATAAGAGAAGGGGAGAAAAGGGTATTGCAGCAAATAATCAAAATTTTCAAAGACAGGGAACTGGAATTAGATGAATTGGAATATTACCAAGAAAGGAGGCTAAAGGATCTTGGGCTATGTGGGGAGCAAGGTGAAATTATCTTTTGGGAGTCCAAATAG

Protein sequence

MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSNVHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANSEIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQLLSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVLIPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNADLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDAMLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTTLEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGLCGEQGEIIFWESK
Homology
BLAST of CmaCh12G007290 vs. ExPASy Swiss-Prot
Match: P94026 (Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic OS=Nicotiana tabacum OX=4097 GN=RBCMT PE=2 SV=1)

HSP 1 Score: 692.6 bits (1786), Expect = 3.3e-198
Identity = 327/451 (72.51%), Postives = 394/451 (87.36%), Query Frame = 0

Query: 45  LNSLASPDLDPSLPSNVHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVL 104
           +NSL   +LDP +P  V TFW+WL +EG+V++KT VKP I PEGLGL   +D++K E VL
Sbjct: 41  INSLQLTELDPKIPQPVQTFWQWLCKEGVVTTKTPVKPGIVPEGLGLVAKRDIAKGETVL 100

Query: 105 EVPKRFWINPDAVANSEIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDST 164
           +VPKRFWINPDAVA SEIGNVCSGLKPWISVALFL+RE +++DS+W+ Y+D+LP+ TDST
Sbjct: 101 QVPKRFWINPDAVAESEIGNVCSGLKPWISVALFLLREKWRDDSKWKYYMDVLPKSTDST 160

Query: 165 VFWSEEELAELQGTQLLSTTLNVKEYVKSEFHKV-EEIILRHKDLFPTPITLDDFFWAFG 224
           ++WSEEEL+E+QGTQLLSTT++VK+YV++EF KV EE+ILR+K LFP PITLDDFFWAFG
Sbjct: 161 IYWSEEELSEIQGTQLLSTTMSVKDYVQNEFQKVEEEVILRNKQLFPFPITLDDFFWAFG 220

Query: 225 ILRSRAFSRLRGQNLVLIPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISV 284
           ILRSRAFSRLR QNL+L+PFADL NH+A VT+E+HA EV+GPAGLFSWD+LFSLRSP+ +
Sbjct: 221 ILRSRAFSRLRNQNLILVPFADLTNHNARVTTEDHAHEVRGPAGLFSWDLLFSLRSPLKL 280

Query: 285 KAGEQVFIQYDLKKSNADLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGL 344
           KAG+Q+FIQYDL KSNAD+ALDYGF+E    R+A+TLTLEI ESD FY DKLDIAETNG+
Sbjct: 281 KAGDQLFIQYDLNKSNADMALDYGFIEPSSARDAFTLTLEISESDEFYGDKLDIAETNGI 340

Query: 345 GETAYFDIVLDRPFPDAMLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVC 404
           GETAYFDI + +  P  M+P+LRL+ALGGTDAFLLES+FRNS+WGHL +PVSRANEE +C
Sbjct: 341 GETAYFDIKIGQSLPPTMIPYLRLVALGGTDAFLLESIFRNSVWGHLGLPVSRANEELIC 400

Query: 405 QVVRNACEVALSGYRTTLEEDEKLKEE-KLDSRLRIAVGIREGEKRVLQQIIKIFKDREL 464
           +VVR+AC+ ALSGY TT+EEDEKL EE  L +RL+IAVGIR GEKRVL+QI  IF++REL
Sbjct: 401 KVVRDACKSALSGYHTTIEEDEKLMEEGNLSTRLQIAVGIRLGEKRVLKQIDDIFREREL 460

Query: 465 ELDELEYYQERRLKDLGLCGEQGEIIFWESK 494
           ELDELEYY ERRLKDLGL GEQG+IIFWE K
Sbjct: 461 ELDELEYYGERRLKDLGLVGEQGDIIFWEPK 491

BLAST of CmaCh12G007290 vs. ExPASy Swiss-Prot
Match: Q43088 (Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic OS=Pisum sativum OX=3888 GN=RBCMT PE=1 SV=1)

HSP 1 Score: 662.5 bits (1708), Expect = 3.6e-189
Identity = 319/461 (69.20%), Postives = 385/461 (83.51%), Query Frame = 0

Query: 29  SFPLKRPLLHPKPRLSLNSLASPDLDPSLPSNVHTFWEWLRQEGLVSSKTHVKPAIFPEG 88
           SF  K P+LH K   S  S+AS   +PSL   V TFW+WL++EG++++KT VK ++  EG
Sbjct: 22  SFTPKAPILHLKRSFSAKSVASVGTEPSLSPAVQTFWKWLQEEGVITAKTPVKASVVTEG 81

Query: 89  LGLATTKDLSKNEVVLEVPKRFWINPDAVANSEIGNVCSGLKPWISVALFLIRENFKEDS 148
           LGL   KD+S+N+V+L+VPKR WINPDAVA SEIG VCS LKPW+SV LFLIRE  +EDS
Sbjct: 82  LGLVALKDISRNDVILQVPKRLWINPDAVAASEIGRVCSELKPWLSVILFLIRERSREDS 141

Query: 149 RWRRYLDILPQETDSTVFWSEEELAELQGTQLLSTTLNVKEYVKSEFHKVE-EIILRHKD 208
            W+ Y  ILPQETDST++WSEEEL ELQG+QLL TT++VKEYVK+E  K+E EIIL +K 
Sbjct: 142 VWKHYFGILPQETDSTIYWSEEELQELQGSQLLKTTVSVKEYVKNECLKLEQEIILPNKR 201

Query: 209 LFPTPITLDDFFWAFGILRSRAFSRLRGQNLVLIPFADLVNHSADVTSEEHAWEVKGPAG 268
           LFP P+TLDDFFWAFGILRSRAFSRLR +NLV++P ADL+NHSA VT+E+HA+EVKG AG
Sbjct: 202 LFPDPVTLDDFFWAFGILRSRAFSRLRNENLVVVPMADLINHSAGVTTEDHAYEVKGAAG 261

Query: 269 LFSWDILFSLRSPISVKAGEQVFIQYDLKKSNADLALDYGFVESKPDRNAYTLTLEIPES 328
           LFSWD LFSL+SP+SVKAGEQV+IQYDL KSNA+LALDYGF+E   +R+AYTLTLEI ES
Sbjct: 262 LFSWDYLFSLKSPLSVKAGEQVYIQYDLNKSNAELALDYGFIEPNENRHAYTLTLEISES 321

Query: 329 DPFYDDKLDIAETNGLGETAYFDIVLDRPFPDAMLPFLRLLALGGTDAFLLESLFRNSIW 388
           DPF+DDKLD+AE+NG  +TAYFDI  +R  P  +LP+LRL+ALGGTDAFLLESLFR++IW
Sbjct: 322 DPFFDDKLDVAESNGFAQTAYFDIFYNRTLPPGLLPYLRLVALGGTDAFLLESLFRDTIW 381

Query: 389 GHLEMPVSRANEEFVCQVVRNACEVALSGYRTTLEEDEKLKEEKLDSRLRIAVGIREGEK 448
           GHLE+ VSR NEE +C+ VR AC+ AL+GY TT+E+D +LKE  LDSRL IAVGIREGEK
Sbjct: 382 GHLELSVSRDNEELLCKAVREACKSALAGYHTTIEQDRELKEGNLDSRLAIAVGIREGEK 441

Query: 449 RVLQQIIKIFKDRELELDELEYYQERRLKDLGLCGEQGEII 489
            VLQQI  IF+ +ELELD+LEYYQERRLKDLGLCGE G+I+
Sbjct: 442 MVLQQIDGIFEQKELELDQLEYYQERRLKDLGLCGENGDIL 482

BLAST of CmaCh12G007290 vs. ExPASy Swiss-Prot
Match: Q9XI84 ([Fructose-bisphosphate aldolase]-lysine N-methyltransferase, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=LSMT-L PE=1 SV=1)

HSP 1 Score: 639.8 bits (1649), Expect = 2.5e-182
Identity = 321/480 (66.88%), Postives = 388/480 (80.83%), Query Frame = 0

Query: 16  SNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSNVHTFWEWLRQEGLVS 75
           S FL +P     I        L  +P+ SL    S      LP NV  FW+WLR +G+VS
Sbjct: 9   SGFLRIP----SIQKSQNPSFLFSRPKKSLVRPISAS-SSELPENVRNFWKWLRDQGVVS 68

Query: 76  SKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANSEIGNVCSGLKPWISV 135
            K+  +PA+ PEGLGL   +D+ +NEVVLE+PKR WINP+ V  S+IG +C GLKPW+SV
Sbjct: 69  GKSVAEPAVVPEGLGLVARRDIGRNEVVLEIPKRLWINPETVTASKIGPLCGGLKPWVSV 128

Query: 136 ALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQLLSTTLNVKEYVKSEF 195
           ALFLIRE ++E+S WR YLD+LPQ TDSTVFWSEEELAEL+GTQLLSTTL VKEYV++EF
Sbjct: 129 ALFLIREKYEEESSWRVYLDMLPQSTDSTVFWSEEELAELKGTQLLSTTLGVKEYVENEF 188

Query: 196 HKVE-EIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVLIPFADLVNHSADVT 255
            K+E EI+L +KDLF + ITLDDF WAFGIL+SRAFSRLRGQNLVLIP ADL+NH+  + 
Sbjct: 189 LKLEQEILLPNKDLFSSRITLDDFIWAFGILKSRAFSRLRGQNLVLIPLADLINHNPAIK 248

Query: 256 SEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNADLALDYGFVESKPD 315
           +E++A+E+KG AGLFS D+LFSL+SP+ VKAGEQV+IQYDL KSNA+LALDYGFVES P 
Sbjct: 249 TEDYAYEIKG-AGLFSRDLLFSLKSPVYVKAGEQVYIQYDLNKSNAELALDYGFVESNPK 308

Query: 316 RNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDAMLPFLRLLALGGTD 375
           RN+YTLT+EIPESDPF+ DKLDIAE+N +GET YFDIV  +  P  ML +LRL+ALGG D
Sbjct: 309 RNSYTLTIEIPESDPFFGDKLDIAESNKMGETGYFDIVDGQTLPAGMLQYLRLVALGGPD 368

Query: 376 AFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTTLEEDEKLKEE-KLD 435
           AFLLES+F N+IWGHLE+PVSR NEE +C+VVR+AC+ ALSG+ TT+EEDEKL ++ KL+
Sbjct: 369 AFLLESIFNNTIWGHLELPVSRTNEELICRVVRDACKSALSGFDTTIEEDEKLLDKGKLE 428

Query: 436 SRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGLCGEQGEIIFWESK 494
            RL +A+ IR GEKRVLQQI +IFKDRELELD LEYYQERRLKDLGL GEQG+IIFWE+K
Sbjct: 429 PRLEMALKIRIGEKRVLQQIDQIFKDRELELDILEYYQERRLKDLGLVGEQGDIIFWETK 482

BLAST of CmaCh12G007290 vs. ExPASy Swiss-Prot
Match: A9X1D0 (Actin-histidine N-methyltransferase OS=Papio anubis OX=9555 GN=SETD3 PE=3 SV=1)

HSP 1 Score: 132.1 bits (331), Expect = 1.7e-29
Identity = 103/393 (26.21%), Postives = 184/393 (46.82%), Query Frame = 0

Query: 87  EGLGLATTKDLSKNEVVLEVPKRFWINPDAVANSEIGNVCSGLKPW-----ISVALFLIR 146
           EG GL  T+D+   E+ L VP++  +  ++  NS +G + S  +       I++A  L+ 
Sbjct: 104 EGFGLRATRDIKAEELFLWVPRKLLMTVESAKNSVLGPLYSQDRILQAMGNIALAFHLLC 163

Query: 147 ENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQLLSTTLNVKEYVKSEFHKVEEI 206
           E    +S W+ Y+  LP E D+ +++ E+E+  LQ TQ +    +  +    ++    ++
Sbjct: 164 ERANPNSFWQPYIQTLPSEYDTPLYFEEDEVRYLQSTQAIHDVFSQYKNTARQYAYFYKV 223

Query: 207 ILRHKDLFPTPI----TLDDFFWAFGILRSRA-------FSRLRGQNLVLIPFADLVNHS 266
           I  H      P+    T +D+ WA   + +R         SR+    L LIP  D+ NH+
Sbjct: 224 IQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQIPTEDGSRV---TLALIPLWDMCNHT 283

Query: 267 ADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNADLALDYGFVE 326
             + +  +  E          D      +    +AGEQ++I Y   +SNA+  +  GF  
Sbjct: 284 NGLITTGYNLE----------DDRCECVALQDFRAGEQIYIFYG-TRSNAEFVIHSGFFF 343

Query: 327 SKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDI-VLDRPFPDAMLPFLRLLA 386
                +   + L + +SD  Y  K ++    G+  ++ F +   + P    +L FLR+  
Sbjct: 344 DNNSHDRVKIKLGVSKSDRLYAMKAEVLARAGIPTSSVFALHFTEPPISAQLLAFLRVFC 403

Query: 387 LGGTDAFLLESLFRNS------IWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTTLEE 446
           +  T+  L E L  +S        G+ E PVS  NE  +   + +   + L  Y+TT+EE
Sbjct: 404 M--TEEELKEHLLGDSAIDRIFTLGNSEFPVSWDNEVKLWTFLEDRASLLLKTYKTTIEE 463

Query: 447 DEK-LKEEKLDSRLRIAVGIREGEKRVLQQIIK 456
           D+  LK + L  R ++A+ +R GEK +L++ +K
Sbjct: 464 DKSVLKNQDLSVRAKMAIKLRLGEKEILEKAVK 480

BLAST of CmaCh12G007290 vs. ExPASy Swiss-Prot
Match: B0VX69 (Actin-histidine N-methyltransferase OS=Callithrix jacchus OX=9483 GN=SETD3 PE=3 SV=2)

HSP 1 Score: 131.7 bits (330), Expect = 2.2e-29
Identity = 104/393 (26.46%), Postives = 183/393 (46.56%), Query Frame = 0

Query: 87  EGLGLATTKDLSKNEVVLEVPKRFWINPDAVANSEIGNVCSGLKPW-----ISVALFLIR 146
           EG GL  T+D+   E+ L VP++  +  ++  NS +G + S  +       I++A  L+ 
Sbjct: 104 EGFGLRATRDIKAEELFLWVPRKLLMTVESAKNSVLGPLYSQDRILQAMGNIALAFHLLC 163

Query: 147 ENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQLLSTTLNVKEYVKSEFHKVEEI 206
           E    +S W+ Y+  LP E D+ +++ EEE+  LQ TQ +    +  +    ++    ++
Sbjct: 164 ERASPNSFWQPYIQTLPSEYDTPLYFEEEEVRYLQSTQAVHDVFSQYKNTARQYAYFYKV 223

Query: 207 ILRHKDLFPTPI----TLDDFFWAFGILRSRA-------FSRLRGQNLVLIPFADLVNHS 266
           I  H      P+    T +D+ WA   + +R         SR+    L LIP  D+ NH+
Sbjct: 224 IQTHPHANKLPLKDSFTYEDYRWAVSSVMTRQNQIPTEDGSRV---TLALIPLWDMCNHT 283

Query: 267 ADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNADLALDYGFVE 326
             + +  +  E          D      +    +AGEQ++I Y   +SNA+  +  GF  
Sbjct: 284 NGLITTGYNLE----------DDRCECVALQDFRAGEQIYIFYG-TRSNAEFVIHSGFFF 343

Query: 327 SKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDI-VLDRPFPDAMLPFLRLLA 386
                +   + L + +SD  Y  K ++    G+  ++ F +   + P    +L FLR+  
Sbjct: 344 DNNSHDRVKIKLGVSKSDRLYAMKAEVLARAGIPTSSVFALHFTEPPISAQLLAFLRVFC 403

Query: 387 LGGTDAFLLESLF------RNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTTLEE 446
           +  T+  L E L       R    G+ E PVS  NE  +   + +   + L  Y+TT+EE
Sbjct: 404 M--TEEELKEHLLGDNAIDRIFTLGNSEFPVSWDNEVKLWTFLEDRASLLLKTYKTTIEE 463

Query: 447 DEK-LKEEKLDSRLRIAVGIREGEKRVLQQIIK 456
           D+  LK + L  R ++A+ +R GEK +L++ +K
Sbjct: 464 DKSVLKNQDLSVRAKMAIKLRLGEKEILEKAVK 480

BLAST of CmaCh12G007290 vs. ExPASy TrEMBL
Match: A0A6J1I899 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111471861 PE=3 SV=1)

HSP 1 Score: 993.4 bits (2567), Expect = 3.3e-286
Identity = 493/493 (100.00%), Postives = 493/493 (100.00%), Query Frame = 0

Query: 1   MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60
           MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN
Sbjct: 1   MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60

Query: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120
           VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS
Sbjct: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120

Query: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180
           EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL
Sbjct: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180

Query: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240
           LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL
Sbjct: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240

Query: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300
           IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA
Sbjct: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300

Query: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360
           DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA
Sbjct: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360

Query: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420
           MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT
Sbjct: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420

Query: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480
           LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL
Sbjct: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480

Query: 481 CGEQGEIIFWESK 494
           CGEQGEIIFWESK
Sbjct: 481 CGEQGEIIFWESK 493

BLAST of CmaCh12G007290 vs. ExPASy TrEMBL
Match: A0A6J1GJ85 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111454704 PE=3 SV=1)

HSP 1 Score: 983.8 bits (2542), Expect = 2.6e-283
Identity = 489/493 (99.19%), Postives = 489/493 (99.19%), Query Frame = 0

Query: 1   MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60
           MATPNPTSM L SSSSNFLSLPRTATCISFPLKRPLLH KPRLSLNSLASPDLDPSLPSN
Sbjct: 1   MATPNPTSMPLPSSSSNFLSLPRTATCISFPLKRPLLHSKPRLSLNSLASPDLDPSLPSN 60

Query: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120
           VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS
Sbjct: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120

Query: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180
           EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL
Sbjct: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180

Query: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240
           LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITL DFFWAFGILRSRAFSRLRGQNLVL
Sbjct: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLHDFFWAFGILRSRAFSRLRGQNLVL 240

Query: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300
           IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA
Sbjct: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300

Query: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360
           DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA
Sbjct: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360

Query: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420
           MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT
Sbjct: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420

Query: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480
           LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL
Sbjct: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480

Query: 481 CGEQGEIIFWESK 494
           CGEQGEIIFWESK
Sbjct: 481 CGEQGEIIFWESK 493

BLAST of CmaCh12G007290 vs. ExPASy TrEMBL
Match: A0A0A0LK02 (SET domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G110250 PE=3 SV=1)

HSP 1 Score: 875.9 bits (2262), Expect = 7.7e-251
Identity = 436/496 (87.90%), Postives = 464/496 (93.55%), Query Frame = 0

Query: 1   MATP--NPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLP 60
           MATP   P  MAL+SSSS+FLSLPRT   +  P KRP     PRLS+ SLASPDLDPSLP
Sbjct: 13  MATPIQTPMGMALASSSSSFLSLPRTPPHLLLPSKRP-----PRLSVTSLASPDLDPSLP 72

Query: 61  SNVHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVA 120
           SNVHTFW+W+RQEG+VS KTHVKPAIFPEGLGLATTK+LSKNEVVLEVPKRFWINPDAVA
Sbjct: 73  SNVHTFWQWVRQEGMVSYKTHVKPAIFPEGLGLATTKNLSKNEVVLEVPKRFWINPDAVA 132

Query: 121 NSEIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGT 180
           +SEIGNVCSGLKPWISVALFLIREN K DSRWRRYLDILPQETDSTVFWSEEELAE+QGT
Sbjct: 133 DSEIGNVCSGLKPWISVALFLIRENLKGDSRWRRYLDILPQETDSTVFWSEEELAEIQGT 192

Query: 181 QLLSTTLNVKEYVKSEFHKV-EEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQN 240
           QLLSTTLNVKEYVKSEF KV EEI+LRHKDLFP+ ITLDDFFWAFGILRSRAFSRLRGQN
Sbjct: 193 QLLSTTLNVKEYVKSEFLKVEEEILLRHKDLFPSRITLDDFFWAFGILRSRAFSRLRGQN 252

Query: 241 LVLIPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKK 300
           LVLIPFADLVNHSA+VT+EEHAWEVKGPAGLFSWD+LFSLRSP+SVKAG+QVFIQYDLKK
Sbjct: 253 LVLIPFADLVNHSANVTTEEHAWEVKGPAGLFSWDVLFSLRSPLSVKAGDQVFIQYDLKK 312

Query: 301 SNADLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPF 360
           SNADLALDYGF+E K DRNAYTLTLEIPESD F+DDKLDIAETNGL +TAYFDI+L+RPF
Sbjct: 313 SNADLALDYGFIEQKSDRNAYTLTLEIPESDLFFDDKLDIAETNGLNQTAYFDIILERPF 372

Query: 361 PDAMLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGY 420
           P AMLPFLRLLALGGTDAFLLESLFRNS+WGHLEMPVSRANEE +CQVVRNACE ALSGY
Sbjct: 373 PPAMLPFLRLLALGGTDAFLLESLFRNSVWGHLEMPVSRANEELICQVVRNACEAALSGY 432

Query: 421 RTTLEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKD 480
            TT+EEDEKLKEE LDSRLRIAVGIREGEKRVLQQII+IFKDRELELD+LEYYQERRLKD
Sbjct: 433 HTTIEEDEKLKEENLDSRLRIAVGIREGEKRVLQQIIQIFKDRELELDQLEYYQERRLKD 492

Query: 481 LGLCGEQGEIIFWESK 494
           LGLCGEQGEIIFWE+K
Sbjct: 493 LGLCGEQGEIIFWETK 503

BLAST of CmaCh12G007290 vs. ExPASy TrEMBL
Match: A0A5A7SQN4 (Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold340G00060 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.3e-250
Identity = 439/496 (88.51%), Postives = 462/496 (93.15%), Query Frame = 0

Query: 1   MATP--NPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLP 60
           MATP   P  MAL+SSSS+FLSLPRT   +  P KRP     PRLS+ SLASPDLDPSLP
Sbjct: 1   MATPITTPMGMALASSSSSFLSLPRTPANLLLPSKRP-----PRLSVTSLASPDLDPSLP 60

Query: 61  SNVHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVA 120
           SNVHTFW+WLRQEG+VSSKTHVKPAIFPEGLGLATTK+LSKNEVVLEVPKRFWINPDAV 
Sbjct: 61  SNVHTFWQWLRQEGVVSSKTHVKPAIFPEGLGLATTKNLSKNEVVLEVPKRFWINPDAVN 120

Query: 121 NSEIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGT 180
           +S IGNVCSGLKPWISVALFLIREN   DSRWRRYLDILPQETDSTVFWSEEELAE+QGT
Sbjct: 121 DSVIGNVCSGLKPWISVALFLIRENLNGDSRWRRYLDILPQETDSTVFWSEEELAEIQGT 180

Query: 181 QLLSTTLNVKEYVKSEFHKV-EEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQN 240
           QLLSTTLNVKEYVKSEF KV EEI+LRHKDLFP+ ITLDDFFWAFGILRSRAFSRLRGQN
Sbjct: 181 QLLSTTLNVKEYVKSEFLKVEEEILLRHKDLFPSQITLDDFFWAFGILRSRAFSRLRGQN 240

Query: 241 LVLIPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKK 300
           LVLIPFADLVNHSA+VTSEEHAWEVKGPAGLFSWD+LFSLRSP+SVKAG+QVFIQYDLKK
Sbjct: 241 LVLIPFADLVNHSANVTSEEHAWEVKGPAGLFSWDVLFSLRSPLSVKAGDQVFIQYDLKK 300

Query: 301 SNADLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPF 360
           SNADLALDYGF+E K DRN YTLTLEIPESD F+ DKLDIAETNGLG+TAYFDIVL+RPF
Sbjct: 301 SNADLALDYGFIEQKSDRNVYTLTLEIPESDLFFADKLDIAETNGLGQTAYFDIVLERPF 360

Query: 361 PDAMLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGY 420
           P AMLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEE VCQVVRNACEVALSGY
Sbjct: 361 PPAMLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEELVCQVVRNACEVALSGY 420

Query: 421 RTTLEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKD 480
            TT+EEDEKLKEE LDSRLRIAVGIREGEKRVLQQII+IF+DRELELD+LEYYQERRLKD
Sbjct: 421 HTTIEEDEKLKEENLDSRLRIAVGIREGEKRVLQQIIQIFRDRELELDQLEYYQERRLKD 480

Query: 481 LGLCGEQGEIIFWESK 494
           LGLCGEQGEIIFWESK
Sbjct: 481 LGLCGEQGEIIFWESK 491

BLAST of CmaCh12G007290 vs. ExPASy TrEMBL
Match: A0A1S3B3Z9 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic OS=Cucumis melo OX=3656 GN=LOC103485765 PE=3 SV=1)

HSP 1 Score: 874.4 bits (2258), Expect = 2.3e-250
Identity = 439/496 (88.51%), Postives = 462/496 (93.15%), Query Frame = 0

Query: 1   MATP--NPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLP 60
           MATP   P  MAL+SSSS+FLSLPRT   +  P KRP     PRLS+ SLASPDLDPSLP
Sbjct: 1   MATPITTPMGMALASSSSSFLSLPRTPANLLLPSKRP-----PRLSVTSLASPDLDPSLP 60

Query: 61  SNVHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVA 120
           SNVHTFW+WLRQEG+VSSKTHVKPAIFPEGLGLATTK+LSKNEVVLEVPKRFWINPDAV 
Sbjct: 61  SNVHTFWQWLRQEGVVSSKTHVKPAIFPEGLGLATTKNLSKNEVVLEVPKRFWINPDAVN 120

Query: 121 NSEIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGT 180
           +S IGNVCSGLKPWISVALFLIREN   DSRWRRYLDILPQETDSTVFWSEEELAE+QGT
Sbjct: 121 DSVIGNVCSGLKPWISVALFLIRENLNGDSRWRRYLDILPQETDSTVFWSEEELAEIQGT 180

Query: 181 QLLSTTLNVKEYVKSEFHKV-EEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQN 240
           QLLSTTLNVKEYVKSEF KV EEI+LRHKDLFP+ ITLDDFFWAFGILRSRAFSRLRGQN
Sbjct: 181 QLLSTTLNVKEYVKSEFLKVEEEILLRHKDLFPSQITLDDFFWAFGILRSRAFSRLRGQN 240

Query: 241 LVLIPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKK 300
           LVLIPFADLVNHSA+VTSEEHAWEVKGPAGLFSWD+LFSLRSP+SVKAG+QVFIQYDLKK
Sbjct: 241 LVLIPFADLVNHSANVTSEEHAWEVKGPAGLFSWDVLFSLRSPLSVKAGDQVFIQYDLKK 300

Query: 301 SNADLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPF 360
           SNADLALDYGF+E K DRN YTLTLEIPESD F+ DKLDIAETNGLG+TAYFDIVL+RPF
Sbjct: 301 SNADLALDYGFIEQKSDRNVYTLTLEIPESDLFFADKLDIAETNGLGQTAYFDIVLERPF 360

Query: 361 PDAMLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGY 420
           P AMLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEE VCQVVRNACEVALSGY
Sbjct: 361 PPAMLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEELVCQVVRNACEVALSGY 420

Query: 421 RTTLEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKD 480
            TT+EEDEKLKEE LDSRLRIAVGIREGEKRVLQQII+IF+DRELELD+LEYYQERRLKD
Sbjct: 421 HTTIEEDEKLKEENLDSRLRIAVGIREGEKRVLQQIIQIFRDRELELDQLEYYQERRLKD 480

Query: 481 LGLCGEQGEIIFWESK 494
           LGLCGEQGEIIFWESK
Sbjct: 481 LGLCGEQGEIIFWESK 491

BLAST of CmaCh12G007290 vs. NCBI nr
Match: XP_022973301.1 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic [Cucurbita maxima])

HSP 1 Score: 993.4 bits (2567), Expect = 6.9e-286
Identity = 493/493 (100.00%), Postives = 493/493 (100.00%), Query Frame = 0

Query: 1   MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60
           MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN
Sbjct: 1   MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60

Query: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120
           VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS
Sbjct: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120

Query: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180
           EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL
Sbjct: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180

Query: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240
           LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL
Sbjct: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240

Query: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300
           IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA
Sbjct: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300

Query: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360
           DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA
Sbjct: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360

Query: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420
           MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT
Sbjct: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420

Query: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480
           LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL
Sbjct: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480

Query: 481 CGEQGEIIFWESK 494
           CGEQGEIIFWESK
Sbjct: 481 CGEQGEIIFWESK 493

BLAST of CmaCh12G007290 vs. NCBI nr
Match: KAG6585869.1 (Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 987.3 bits (2551), Expect = 4.9e-284
Identity = 490/493 (99.39%), Postives = 490/493 (99.39%), Query Frame = 0

Query: 1   MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60
           MATPNPT M L SSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN
Sbjct: 1   MATPNPTYMPLPSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60

Query: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120
           VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS
Sbjct: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120

Query: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180
           EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL
Sbjct: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180

Query: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240
           LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL
Sbjct: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240

Query: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300
           IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA
Sbjct: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300

Query: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360
           DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA
Sbjct: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360

Query: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420
           MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT
Sbjct: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420

Query: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480
           LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL
Sbjct: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480

Query: 481 CGEQGEIIFWESK 494
           CGEQGEIIFWESK
Sbjct: 481 CGEQGEIIFWESK 493

BLAST of CmaCh12G007290 vs. NCBI nr
Match: KAG7020773.1 (Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 986.1 bits (2548), Expect = 1.1e-283
Identity = 489/493 (99.19%), Postives = 489/493 (99.19%), Query Frame = 0

Query: 1   MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60
           MATPNPTSM L SSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN
Sbjct: 6   MATPNPTSMPLPSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 65

Query: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120
           VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGL TTKDLSKNEVVLEVPKRFWINPDAVANS
Sbjct: 66  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLVTTKDLSKNEVVLEVPKRFWINPDAVANS 125

Query: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180
           EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL
Sbjct: 126 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 185

Query: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240
           LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL
Sbjct: 186 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 245

Query: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300
           IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA
Sbjct: 246 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 305

Query: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360
           DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLD PFPDA
Sbjct: 306 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDHPFPDA 365

Query: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420
           MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT
Sbjct: 366 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 425

Query: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480
           LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL
Sbjct: 426 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 485

Query: 481 CGEQGEIIFWESK 494
           CGEQGEIIFWESK
Sbjct: 486 CGEQGEIIFWESK 498

BLAST of CmaCh12G007290 vs. NCBI nr
Match: XP_023538470.1 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 985.3 bits (2546), Expect = 1.9e-283
Identity = 488/493 (98.99%), Postives = 490/493 (99.39%), Query Frame = 0

Query: 1   MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60
           MATPNPTS+ L SSSSNFLSLPRTATCI FPLKRPLLHPKPRLSLNSLASPDLDPSLPSN
Sbjct: 1   MATPNPTSLPLPSSSSNFLSLPRTATCIPFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60

Query: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120
           VHTFWEWLRQEGL+SSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS
Sbjct: 61  VHTFWEWLRQEGLLSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120

Query: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180
           EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL
Sbjct: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180

Query: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240
           LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL
Sbjct: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240

Query: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300
           IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA
Sbjct: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300

Query: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360
           DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA
Sbjct: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360

Query: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420
           MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT
Sbjct: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420

Query: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480
           LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL
Sbjct: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480

Query: 481 CGEQGEIIFWESK 494
           CGEQGEIIFWESK
Sbjct: 481 CGEQGEIIFWESK 493

BLAST of CmaCh12G007290 vs. NCBI nr
Match: XP_022951988.1 (ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 983.8 bits (2542), Expect = 5.4e-283
Identity = 489/493 (99.19%), Postives = 489/493 (99.19%), Query Frame = 0

Query: 1   MATPNPTSMALSSSSSNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSN 60
           MATPNPTSM L SSSSNFLSLPRTATCISFPLKRPLLH KPRLSLNSLASPDLDPSLPSN
Sbjct: 1   MATPNPTSMPLPSSSSNFLSLPRTATCISFPLKRPLLHSKPRLSLNSLASPDLDPSLPSN 60

Query: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120
           VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS
Sbjct: 61  VHTFWEWLRQEGLVSSKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANS 120

Query: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180
           EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL
Sbjct: 121 EIGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQL 180

Query: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVL 240
           LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITL DFFWAFGILRSRAFSRLRGQNLVL
Sbjct: 181 LSTTLNVKEYVKSEFHKVEEIILRHKDLFPTPITLHDFFWAFGILRSRAFSRLRGQNLVL 240

Query: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300
           IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA
Sbjct: 241 IPFADLVNHSADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNA 300

Query: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360
           DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA
Sbjct: 301 DLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDA 360

Query: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420
           MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT
Sbjct: 361 MLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTT 420

Query: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480
           LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL
Sbjct: 421 LEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGL 480

Query: 481 CGEQGEIIFWESK 494
           CGEQGEIIFWESK
Sbjct: 481 CGEQGEIIFWESK 493

BLAST of CmaCh12G007290 vs. TAIR 10
Match: AT1G14030.1 (Rubisco methyltransferase family protein )

HSP 1 Score: 639.8 bits (1649), Expect = 1.8e-183
Identity = 321/480 (66.88%), Postives = 388/480 (80.83%), Query Frame = 0

Query: 16  SNFLSLPRTATCISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSNVHTFWEWLRQEGLVS 75
           S FL +P     I        L  +P+ SL    S      LP NV  FW+WLR +G+VS
Sbjct: 9   SGFLRIP----SIQKSQNPSFLFSRPKKSLVRPISAS-SSELPENVRNFWKWLRDQGVVS 68

Query: 76  SKTHVKPAIFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANSEIGNVCSGLKPWISV 135
            K+  +PA+ PEGLGL   +D+ +NEVVLE+PKR WINP+ V  S+IG +C GLKPW+SV
Sbjct: 69  GKSVAEPAVVPEGLGLVARRDIGRNEVVLEIPKRLWINPETVTASKIGPLCGGLKPWVSV 128

Query: 136 ALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQLLSTTLNVKEYVKSEF 195
           ALFLIRE ++E+S WR YLD+LPQ TDSTVFWSEEELAEL+GTQLLSTTL VKEYV++EF
Sbjct: 129 ALFLIREKYEEESSWRVYLDMLPQSTDSTVFWSEEELAELKGTQLLSTTLGVKEYVENEF 188

Query: 196 HKVE-EIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVLIPFADLVNHSADVT 255
            K+E EI+L +KDLF + ITLDDF WAFGIL+SRAFSRLRGQNLVLIP ADL+NH+  + 
Sbjct: 189 LKLEQEILLPNKDLFSSRITLDDFIWAFGILKSRAFSRLRGQNLVLIPLADLINHNPAIK 248

Query: 256 SEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNADLALDYGFVESKPD 315
           +E++A+E+KG AGLFS D+LFSL+SP+ VKAGEQV+IQYDL KSNA+LALDYGFVES P 
Sbjct: 249 TEDYAYEIKG-AGLFSRDLLFSLKSPVYVKAGEQVYIQYDLNKSNAELALDYGFVESNPK 308

Query: 316 RNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDIVLDRPFPDAMLPFLRLLALGGTD 375
           RN+YTLT+EIPESDPF+ DKLDIAE+N +GET YFDIV  +  P  ML +LRL+ALGG D
Sbjct: 309 RNSYTLTIEIPESDPFFGDKLDIAESNKMGETGYFDIVDGQTLPAGMLQYLRLVALGGPD 368

Query: 376 AFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNACEVALSGYRTTLEEDEKLKEE-KLD 435
           AFLLES+F N+IWGHLE+PVSR NEE +C+VVR+AC+ ALSG+ TT+EEDEKL ++ KL+
Sbjct: 369 AFLLESIFNNTIWGHLELPVSRTNEELICRVVRDACKSALSGFDTTIEEDEKLLDKGKLE 428

Query: 436 SRLRIAVGIREGEKRVLQQIIKIFKDRELELDELEYYQERRLKDLGLCGEQGEIIFWESK 494
            RL +A+ IR GEKRVLQQI +IFKDRELELD LEYYQERRLKDLGL GEQG+IIFWE+K
Sbjct: 429 PRLEMALKIRIGEKRVLQQIDQIFKDRELELDILEYYQERRLKDLGLVGEQGDIIFWETK 482

BLAST of CmaCh12G007290 vs. TAIR 10
Match: AT3G55080.1 (SET domain-containing protein )

HSP 1 Score: 104.0 bits (258), Expect = 3.5e-22
Identity = 87/327 (26.61%), Postives = 150/327 (45.87%), Query Frame = 0

Query: 28  ISFPLKRPLLHPKPRLSLNSLASPDLDPSLPSNVHTFWEWLRQEGLVSSKTHVKPAIFPE 87
           +S   KR  L  K  L L + AS D         + F  WL +         +       
Sbjct: 19  VSSLAKRFSLAGKLTLELQTQASLD---------NNFLPWLERIAGAKITNTLSIGKSTY 78

Query: 88  GLGLATTKDLSKNEVVLEVPKRFWINPD-------AVANSEIGNVCSGLKPWISVALFLI 147
           G  L  +K +   + +L+VP    I PD        + ++E+GN+  G+     +A  LI
Sbjct: 79  GRSLFASKVIYAGDCMLKVPFNAQITPDELPSDIRVLLSNEVGNI--GM-----LAAVLI 138

Query: 148 RE-NFKEDSRWRRYLDILPQ--ETDSTVFWSEEELAELQGTQLLSTTLNVKEYVKSEFHK 207
           RE    + SRW  Y+  LPQ  E  S++FW E+EL+ ++ + +   T+  K  ++ +F  
Sbjct: 139 REKKMGQKSRWVPYISRLPQPAEMHSSIFWGEDELSMIRCSAVHQETVKQKAQIEKDFSF 198

Query: 208 VEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVLIPFADLVNH---SADVT 267
           V +   +H  +      L+DF +A+ ++ SRA+     + + LIPFAD +NH   SA + 
Sbjct: 199 VAQAFKQHCPIVTERPDLEDFMYAYALVGSRAWE--NSKRISLIPFADFMNHDGLSASIV 258

Query: 268 SEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNADLALDYGFVESKPD 327
             +              + L  + +  +   G++VFI+Y  + SNA L LD+GF      
Sbjct: 259 LRDED------------NQLSEVTADRNYSPGDEVFIKYG-EFSNATLMLDFGFTFPYNI 314

Query: 328 RNAYTLTLEIPESDPFYDDKLDIAETN 342
            +   + +++P  DP  + KL + +T+
Sbjct: 319 HDEVQIQMDVPNDDPLRNMKLGLLQTH 314

BLAST of CmaCh12G007290 vs. TAIR 10
Match: AT3G55080.2 (SET domain-containing protein )

HSP 1 Score: 93.2 bits (230), Expect = 6.3e-19
Identity = 61/213 (28.64%), Postives = 110/213 (51.64%), Query Frame = 0

Query: 135 VALFLIRE-NFKEDSRWRRYLDILPQ--ETDSTVFWSEEELAELQGTQLLSTTLNVKEYV 194
           +A  LIRE    + SRW  Y+  LPQ  E  S++FW E+EL+ ++ + +   T+  K  +
Sbjct: 2   LAAVLIREKKMGQKSRWVPYISRLPQPAEMHSSIFWGEDELSMIRCSAVHQETVKQKAQI 61

Query: 195 KSEFHKVEEIILRHKDLFPTPITLDDFFWAFGILRSRAFSRLRGQNLVLIPFADLVNH-- 254
           + +F  V +   +H  +      L+DF +A+ ++ SRA+     + + LIPFAD +NH  
Sbjct: 62  EKDFSFVAQAFKQHCPIVTERPDLEDFMYAYALVGSRAWE--NSKRISLIPFADFMNHDG 121

Query: 255 -SADVTSEEHAWEVKGPAGLFSWDILFSLRSPISVKAGEQVFIQYDLKKSNADLALDYGF 314
            SA +   +   ++   + L        + +  +   G++VFI+Y  + SNA L LD+GF
Sbjct: 122 LSASIVLRDEDNQLSEFSTL-------QVTADRNYSPGDEVFIKYG-EFSNATLMLDFGF 181

Query: 315 VESKPDRNAYTLTLEIPESDPFYDDKLDIAETN 342
                  +   + +++P  DP  + KL + +T+
Sbjct: 182 TFPYNIHDEVQIQMDVPNDDPLRNMKLGLLQTH 204

BLAST of CmaCh12G007290 vs. TAIR 10
Match: AT2G18850.1 (SET domain-containing protein )

HSP 1 Score: 79.3 bits (194), Expect = 9.4e-15
Identity = 89/404 (22.03%), Postives = 171/404 (42.33%), Query Frame = 0

Query: 66  EWLRQEGLVSSKTHVKPA-IFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANSE--- 125
           EW +  G+   KT ++ A I   G G   ++DL   +V LE+P    I+ + V NS+   
Sbjct: 155 EWGQDNGV---KTKLQIAQIDGYGRGAIASEDLKFGDVALEIPVSSIISEEYVYNSDMYP 214

Query: 126 IGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQLL 185
           I     G+     + L+ +RE    DS+++ Y D L +   + + +  + + EL GT LL
Sbjct: 215 ILETFDGITSETMLLLWTMREKHNLDSKFKPYFDSLQENFCTGLSFGVDAIMELDGTLLL 274

Query: 186 STTLNVKEYVKSEFHKVEEIILRHKDLFPTPI-TLDDFFWAFGILRSRAF------SRLR 245
              +  KE ++  + ++  ++  H+++FP  + T + + WA  +  S +        +L+
Sbjct: 275 DEIMQAKELLRERYDELIPLLSNHREVFPPELYTWEHYLWACELYYSNSMQIKFPDGKLK 334

Query: 246 GQNLVLIPFADLVNHSADVTSEEHAWEVK-GPAGLFSWDILFSLRSPISVKAGEQVFIQY 305
                LIP A  +NHS       +   VK G   + +  + F +  P +   GEQ F+ Y
Sbjct: 335 ---TCLIPVAGFLNHSI------YPHIVKYGKVDIETSSLKFPVSRPCN--KGEQCFLSY 394

Query: 306 DLKKSNADLALDYGFVESKPDRNAYTLTLEIPESDPFYDDKLDIAETNGLGETAYFDI-- 365
               S++ L   YGF+  K D     + L+    D   D + + + T  +    +     
Sbjct: 395 G-NYSSSHLLTFYGFL-PKGDNPYDVIPLDFDVIDD-EDIETEFSWTTHMLRGTWLSSNH 454

Query: 366 -VLDRPFPDAMLPFLRLLALGGTDAFLLESLFRNSIWGHLEMPVSRANEEFVCQVVRNAC 425
            +     P  +L +LR        A  L       +W +LE+ +           V    
Sbjct: 455 NIFHYGLPTPLLNYLR-------KAHGLVHHSETDLWKNLEVEIG----------VLENL 514

Query: 426 EVALSGYRTTLEEDEKLKEEKLDSRLRIAVGIREGEKRVLQQII 455
           +         L + + +  E  D  +++A+  +E +++++  I+
Sbjct: 515 QSTFDDMMQNLGDADSIDRENADWDVKLAMEFKERQRKIVSSIL 524

BLAST of CmaCh12G007290 vs. TAIR 10
Match: AT2G18850.2 (SET domain-containing protein )

HSP 1 Score: 79.0 bits (193), Expect = 1.2e-14
Identity = 67/256 (26.17%), Postives = 120/256 (46.88%), Query Frame = 0

Query: 66  EWLRQEGLVSSKTHVKPA-IFPEGLGLATTKDLSKNEVVLEVPKRFWINPDAVANSE--- 125
           EW +  G+   KT ++ A I   G G   ++DL   +V LE+P    I+ + V NS+   
Sbjct: 155 EWGQDNGV---KTKLQIAQIDGYGRGAIASEDLKFGDVALEIPVSSIISEEYVYNSDMYP 214

Query: 126 IGNVCSGLKPWISVALFLIRENFKEDSRWRRYLDILPQETDSTVFWSEEELAELQGTQLL 185
           I     G+     + L+ +RE    DS+++ Y D L +   + + +  + + EL GT LL
Sbjct: 215 ILETFDGITSETMLLLWTMREKHNLDSKFKPYFDSLQENFCTGLSFGVDAIMELDGTLLL 274

Query: 186 STTLNVKEYVKSEFHKVEEIILRHKDLFPTPI-TLDDFFWAFGILRSRAF------SRLR 245
              +  KE ++  + ++  ++  H+++FP  + T + + WA  +  S +        +L+
Sbjct: 275 DEIMQAKELLRERYDELIPLLSNHREVFPPELYTWEHYLWACELYYSNSMQIKFPDGKLK 334

Query: 246 GQNLVLIPFADLVNHSADVTSEEHAWEVK-GPAGLFSWDILFSLRSPISVKAGEQVFIQY 305
                LIP A  +NHS       +   VK G   + +  + F +  P +   GEQ F+ Y
Sbjct: 335 ---TCLIPVAGFLNHSI------YPHIVKYGKVDIETSSLKFPVSRPCN--KGEQCFLSY 394

Query: 306 DLKKSNADLALDYGFV 310
               S++ L   YGF+
Sbjct: 395 G-NYSSSHLLTFYGFL 395

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P940263.3e-19872.51Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
Q430883.6e-18969.20Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
Q9XI842.5e-18266.88[Fructose-bisphosphate aldolase]-lysine N-methyltransferase, chloroplastic OS=Ar... [more]
A9X1D01.7e-2926.21Actin-histidine N-methyltransferase OS=Papio anubis OX=9555 GN=SETD3 PE=3 SV=1[more]
B0VX692.2e-2926.46Actin-histidine N-methyltransferase OS=Callithrix jacchus OX=9483 GN=SETD3 PE=3 ... [more]
Match NameE-valueIdentityDescription
A0A6J1I8993.3e-286100.00ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
A0A6J1GJ852.6e-28399.19ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
A0A0A0LK027.7e-25187.90SET domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G110250 PE=3 SV... [more]
A0A5A7SQN42.3e-25088.51Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
A0A1S3B3Z92.3e-25088.51ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
Match NameE-valueIdentityDescription
XP_022973301.16.9e-286100.00ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
KAG6585869.14.9e-28499.39Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
KAG7020773.11.1e-28399.19Ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
XP_023538470.11.9e-28398.99ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
XP_022951988.15.4e-28399.19ribulose-1,5 bisphosphate carboxylase/oxygenase large subunit N-methyltransferas... [more]
Match NameE-valueIdentityDescription
AT1G14030.11.8e-18366.88Rubisco methyltransferase family protein [more]
AT3G55080.13.5e-2226.61SET domain-containing protein [more]
AT3G55080.26.3e-1928.64SET domain-containing protein [more]
AT2G18850.19.4e-1522.03SET domain-containing protein [more]
AT2G18850.21.2e-1426.17SET domain-containing protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001214SET domainSMARTSM00317set_7coord: 76..300
e-value: 5.1E-7
score: 39.3
IPR001214SET domainPFAMPF00856SETcoord: 88..293
e-value: 9.0E-9
score: 36.0
IPR001214SET domainPROSITEPS50280SETcoord: 71..294
score: 11.267056
NoneNo IPR availableGENE3D3.90.1410.10set domain protein methyltransferase, domain 1coord: 57..309
e-value: 3.6E-76
score: 257.9
NoneNo IPR availablePIRSRPIRSR009328-1PIRSR009328-1coord: 55..490
e-value: 8.5E-187
score: 618.5
NoneNo IPR availablePANTHERPTHR13271UNCHARACTERIZED PUTATIVE METHYLTRANSFERASEcoord: 41..486
NoneNo IPR availablePANTHERPTHR13271:SF113[FRUCTOSE-BISPHOSPHATE ALDOLASE]-LYSINE N-METHYLTRANSFERASE, CHLOROPLASTICcoord: 41..486
NoneNo IPR availableSUPERFAMILY82199SET domaincoord: 60..314
IPR015353Rubisco LSMT, substrate-binding domainPFAMPF09273Rubis-subs-bindcoord: 327..450
e-value: 6.5E-28
score: 98.0
IPR011192Rubisco LSMT methyltransferase, plantPIRSFPIRSF009328RMT_SETcoord: 5..493
e-value: 3.6E-234
score: 775.5
IPR011192Rubisco LSMT methyltransferase, plantPROSITEPS51583SAM_MT127coord: 29..493
score: 94.934113
IPR036464Rubisco LSMT, substrate-binding domain superfamilyGENE3D3.90.1420.10coord: 310..474
e-value: 6.5E-66
score: 223.1
IPR036464Rubisco LSMT, substrate-binding domain superfamilySUPERFAMILY81822RuBisCo LSMT C-terminal, substrate-binding domaincoord: 317..490
IPR044431RBCMT, SET domainCDDcd19179SET_RBCMTcoord: 70..309
e-value: 2.32437E-85
score: 261.094

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G007290.1CmaCh12G007290.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0018026 peptidyl-lysine monomethylation
biological_process GO:0018022 peptidyl-lysine methylation
cellular_component GO:0009507 chloroplast
molecular_function GO:0005515 protein binding
molecular_function GO:0016279 protein-lysine N-methyltransferase activity
molecular_function GO:0030785 [ribulose-bisphosphate carboxylase]-lysine N-methyltransferase activity