CmaCh12G006580 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh12G006580 vs. ExPASy Swiss-Prot
Match: Q8LGN0 (Glutamate receptor 2.7 OS=Arabidopsis thaliana OX=3702 GN=GLR2.7 PE=2 SV=3)

HSP 1 Score: 816.6 bits (2108), Expect = 2.9e-235
Identity = 435/900 (48.33%), Postives = 592/900 (65.78%), Query Frame = 0

Query: 13  FIAGCLLLVATAGEAQ--NVSVGVVLDMESWVGKVGLSCIDMSLSEFYEANPHYNTRVVL 72
           F+ G +L+    G+ Q   + VGVVLD+ +   K+ L+ I++SLS+FY+ +  Y TR+ +
Sbjct: 20  FVCGFVLMEGCLGQNQTTEIKVGVVLDLHTSFSKLCLTSINISLSDFYKYHSDYTTRLAI 79

Query: 73  HRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVPILTFTASSPS 132
           H RDS  DVV A++AALDLIKN +V AI+GP TSMQ  F+I+LA  S VP +TF+A+ P 
Sbjct: 80  HIRDSMEDVVQASSAALDLIKNEQVSAIIGPRTSMQAEFMIRLADKSQVPTITFSATCPL 139

Query: 133 LASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILPYLIDALQAVN 192
           L S  SPYF R T  DS+QV AI+ +VK + WR VV IY D+EFG+GILP L DALQ V 
Sbjct: 140 LTSINSPYFVRATLDDSSQVKAIAAIVKSFGWRNVVAIYVDNEFGEGILPLLTDALQDVQ 199

Query: 193 ARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKANEIGMMREGYV 252
           A V+ R +I     +DQI +ELYKLMTM TRV +VHM P+L  R F KA EIGMM EGYV
Sbjct: 200 AFVVNRCLIPQEANDDQILKELYKLMTMQTRVFVVHMPPTLGFRFFQKAREIGMMEEGYV 259

Query: 253 WILTDATTNLLGSMS-SSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFVMENSVLNDP 312
           W+LTD   NLL S    S L++M+G LGVR+++PKS +L  F+++W++ F  +    ND 
Sbjct: 260 WLLTDGVMNLLKSNERGSSLENMQGVLGVRSHIPKSKKLKNFRLRWEKMFPKKG---NDE 319

Query: 313 HLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRS-ENLTDLQTLGVSRNGEKIGEALS 372
            ++IF L AYD+  ALA+A+EKT +K+  ++ P  S  N T+L TLGVSR G  + +ALS
Sbjct: 320 EMNIFALRAYDSITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALS 379

Query: 373 KTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETMT------- 432
             +F GL G +E++NGQL+S+ F+++N+  +    +G W P  G++++    T       
Sbjct: 380 NVRFNGLAGEFELINGQLESSVFDVINIIGSEERIIGLWRPSNGIVNAKSKNTTSVLGER 439

Query: 433 ---VIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVE------GYCKDV 492
              VIWPG +  VPKGW   T GK LR+G+PVKKG++EF+  K   +       GYC ++
Sbjct: 440 LGPVIWPGKSKDVPKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEI 499

Query: 493 FDAAIATLPYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVD 552
           F+A +  LPY V   YI F     S   +YDE++ QVY G YD  VGD+TIVANRS YVD
Sbjct: 500 FEAVLKKLPYSVIPKYIAFL----SPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVD 559

Query: 553 FTLPFTESGVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPD 612
           FTLP+TESGVSM+VP + N     W+FL+P +LDLW+T+ CFFVFIGF+VWILEHR+N D
Sbjct: 560 FTLPYTESGVSMMVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTD 619

Query: 613 FRGPPTHQISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLT 672
           FRGPP HQI TS W++F TM FA RE ++SNLARFVV++W FVV VL QSYTA+LTS  T
Sbjct: 620 FRGPPHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFT 679

Query: 673 VQELQPSITNINELLTKQSWVGYLEGSFVWGLLNSVGI--KNLKSYKCPEELDKLLKLGG 732
           V+ LQP++TN  +L+     +GY  G+FV  LL S G     LK +    E D+L     
Sbjct: 680 VKLLQPTVTNWKDLIKFNKNIGYQRGTFVRELLKSQGFDESQLKPFGSAVECDELF---- 739

Query: 733 SNGGIDAAFDETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVT 792
           SNG I A+FDE  Y+K+ LS+  +KY   +P++KT GFGF F   SPL  D+SRA+LNVT
Sbjct: 740 SNGTITASFDEVAYIKVILSQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNVT 799

Query: 793 ESRKMNQLQMKWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLR 852
           +  +M  ++ KWF    N       ++S+ L+L SFWGLFLI G AS ++L I+   FL 
Sbjct: 800 QGEEMQHIENKWFKKPNNCPDLNTSLSSNHLSLSSFWGLFLIAGIASFLALLIFVANFLY 859

Query: 853 KEQHTLHHTANEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPV 891
           + +HTL     + S +S R K++ L++ +D++D+ S+ F K N    V + I     +P+
Sbjct: 860 EHKHTLF----DDSENSFRGKLKFLVRNFDEKDIKSHMF-KENAVHNVSSPITQGSSSPL 901

BLAST of CmaCh12G006580 vs. ExPASy Swiss-Prot
Match: O81078 (Glutamate receptor 2.9 OS=Arabidopsis thaliana OX=3702 GN=GLR2.9 PE=2 SV=1)

HSP 1 Score: 811.6 bits (2095), Expect = 9.3e-234
Identity = 427/878 (48.63%), Postives = 595/878 (67.77%), Query Frame = 0

Query: 12  WFIAGCLLLVATAGEAQ--NVSVGVVLDMESWVGKVGLSCIDMSLSEFYEANPHYNTRVV 71
           +F+ G LL+    G+ Q   + VGVVLD+ +   K+ L+ I M++S+FY  +P+Y TR+ 
Sbjct: 10  YFVCGFLLMGVGLGQNQTSEIKVGVVLDLNTTFSKICLTSIKMAVSDFYADHPNYLTRLT 69

Query: 72  LHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVPILTFTASSP 131
           LH RDS  D V A+AAALDLIK  +V AI+GP  SMQ +F+IKLA+ + VP +TF+A+SP
Sbjct: 70  LHVRDSMEDTVQASAAALDLIKTEQVSAIIGPINSMQADFMIKLANKTQVPTITFSATSP 129

Query: 132 SLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILPYLIDALQAV 191
            L S +SPYF R T  DS+QV AI+ + KF+ WR+VV IY D+EFG+G +P+L DALQ V
Sbjct: 130 LLTSIKSPYFVRATIDDSSQVRAIASIFKFFRWRRVVAIYVDNEFGEGFMPFLFDALQDV 189

Query: 192 NARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKANEIGMMREGY 251
             +   RSVI P   +D+I++EL KLM    RV +VHM  SLA+R+F  A +IGMM EGY
Sbjct: 190 EVK---RSVIPPEAIDDEIQKELRKLMERQARVFVVHMESSLALRVFQIARDIGMMEEGY 249

Query: 252 VWILTDATTNLLGSMSSS-VLKSMEGALGVRTYVPKSMELDRFKIKWKREFVMENSVLND 311
           VW++T+  T+++  +++   L ++EG LGVR++VPKS EL  F+++WKR F  EN  + D
Sbjct: 250 VWLMTNGMTHMMRHINNGRSLNTIEGVLGVRSHVPKSKELGDFRLRWKRTFEKENPSMRD 309

Query: 312 PHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNR-SENLTDLQTLGVSRNGEKIGEAL 371
             L++F LWAYD+  ALA A+EK   K+  +++ +  S+N TDL  +GVS  G  + +A 
Sbjct: 310 -DLNVFALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSLQKAF 369

Query: 372 SKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLL----SSSETM-TV 431
           S+ +F GL G +++++GQLQS  FEI+N   N    +GFW P  GL+    S+ +T+  V
Sbjct: 370 SEVRFNGLAGEFKLIDGQLQSPKFEIINFVGNEERIIGFWTPRDGLMDATSSNKKTLGPV 429

Query: 432 IWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEV------EGYCKDVFDAA 491
           IWPG +  VPKGW+    GK+LR+GVP+KKG+ +F+KV    +       GY  ++F+AA
Sbjct: 430 IWPGKSKIVPKGWEI--PGKKLRVGVPMKKGFFDFVKVTINPITNKKTPTGYAIEIFEAA 489

Query: 492 IATLPYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLP 551
           +  LPY V  +Y+ F   N     +Y+ L+ QVY   +D  VGD+TI ANRS Y DFTLP
Sbjct: 490 LKELPYLVIPEYVSFESPN-----NYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLP 549

Query: 552 FTESGVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGP 611
           FTESGVSM+VP + N     W+FL+P +L+LW+T+ CFFVFIGFVVW+ EHR+N DFRGP
Sbjct: 550 FTESGVSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGP 609

Query: 612 PTHQISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQEL 671
           P +QI TSLW+SF TMVFA RE ++SNLARFVVV+W FVV VLTQSYTASLTS LTVQ L
Sbjct: 610 PQYQIGTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSL 669

Query: 672 QPSITNINELLTKQSWVGYLEGSFVWGLLNSVGI--KNLKSYKCPEELDKLLKLGGSNGG 731
           QP++TN+N+L+  +  VGY  G+FV  +L  +G     LK +   ++ D LL  G S  G
Sbjct: 670 QPTVTNVNDLIKNRDCVGYQGGAFVKDILLGLGFHEDQLKPFDSAKDADDLLSKGKSK-G 729

Query: 732 IDAAFDETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRK 791
           I AAFDE  YLK  LS+  +KY+  +PT+KT GFGFAF   SPL  + SRA+LN+T++  
Sbjct: 730 IAAAFDEVAYLKAILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNV 789

Query: 792 MNQLQMKWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQH 851
             Q++ +WF  + +    +  ++S+RLNL SF GLFLI GTA   SL ++  +FL + +H
Sbjct: 790 TQQIEDRWFPKKNDCPDPMTALSSNRLNLSSFLGLFLIAGTAISFSLLVFVALFLYEHRH 849

Query: 852 TLHHTANEGSNSSIRSKIRALLKIYDKRDLTSYTFRKS 873
           TL     + S  S+  K++ L KI+D++D+ S+TF+ S
Sbjct: 850 TL----GDDSEDSLWRKLKFLFKIFDEKDMNSHTFKNS 871

BLAST of CmaCh12G006580 vs. ExPASy Swiss-Prot
Match: Q9C5V5 (Glutamate receptor 2.8 OS=Arabidopsis thaliana OX=3702 GN=GLR2.8 PE=2 SV=2)

HSP 1 Score: 810.1 bits (2091), Expect = 2.7e-233
Identity = 442/936 (47.22%), Postives = 596/936 (63.68%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQN----VSVGVVLDMESWVGKVGLSCIDMSLSE 60
           M P + +   L +   CL L+   G  QN    + VGVVLD+ +   K+ L+ I+++LS+
Sbjct: 1   MNPKKNNNTFLSYFV-CLFLLLEVGLGQNQISEIKVGVVLDLNTTFSKICLTSINLALSD 60

Query: 61  FYEANPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHN 120
           FY+ +P+Y TR+ LH RDS  D V A+AAALDLI+N +V AI+GP  SMQ  F+IKLA+ 
Sbjct: 61  FYKDHPNYRTRLALHVRDSMKDTVQASAAALDLIQNEQVSAIIGPIDSMQAKFMIKLANK 120

Query: 121 SHVPILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGD 180
           + VP ++F+A+SP L S +S YF R T  DS QV AI+ + + + WR VV IY D+E G+
Sbjct: 121 TQVPTISFSATSPLLTSIKSDYFVRGTIDDSYQVKAIAAIFESFGWRSVVAIYVDNELGE 180

Query: 181 GILPYLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLF 240
           GI+PYL DALQ V    + RSVI     +DQI +ELYKLMT  TRV +VHM   LA R+F
Sbjct: 181 GIMPYLFDALQDVQ---VDRSVIPSEANDDQILKELYKLMTRQTRVFVVHMASRLASRIF 240

Query: 241 AKANEIGMMREGYVWILTDATTNLLGSM-SSSVLKSMEGALGVRTYVPKSMELDRFKIKW 300
            KA EIGMM EGYVW++T+  T+++  +     L +++G LGVR++VPKS  L+ F+++W
Sbjct: 241 EKATEIGMMEEGYVWLMTNGMTHMMRHIHHGRSLNTIDGVLGVRSHVPKSKGLEDFRLRW 300

Query: 301 KREFVMENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPN-RSENLTDLQTL 360
           KR F  EN  L D  L IFGLWAYD+  ALA+A+EKT + +F + + +  S N+TDL TL
Sbjct: 301 KRNFKKENPWLRD-DLSIFGLWAYDSTTALAMAVEKTNISSFPYNNASGSSNNMTDLGTL 360

Query: 361 GVSRNGEKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLL 420
            VSR G  + EALS+ +F GL G + +++ QL+S  FEI+N   N    VGFW P  GL+
Sbjct: 361 HVSRYGPSLLEALSEIRFNGLAGRFNLIDRQLESPKFEIINFVGNEERIVGFWTPSNGLV 420

Query: 421 SSSETMT----------VIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKV---- 480
           + +   T          +IWPG +T VPKGW+  T GK++++GVPVKKG+  F++V    
Sbjct: 421 NVNSNKTTSFTGERFGPLIWPGKSTIVPKGWEIPTNGKKIKVGVPVKKGFFNFVEVITDP 480

Query: 481 --KGTEVEGYCKDVFDAAIATLPYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAV 540
               T  +GY  D+F+AA+  LPY V   Y  F     S    YD+L+ +V  G  D  V
Sbjct: 481 ITNITTPKGYAIDIFEAALKKLPYSVIPQYYRFE----SPDDDYDDLVYKVDNGTLDAVV 540

Query: 541 GDLTIVANRSKYVDFTLPFTESGVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFI 600
           GD+TI A RS Y DFTLP+TESGVSM+VP + N     W+FLKP  LDLW+T+ CFFV I
Sbjct: 541 GDVTITAYRSLYADFTLPYTESGVSMMVPVRDNENKNTWVFLKPWGLDLWVTTACFFVLI 600

Query: 601 GFVVWILEHRINPDFRGPPTHQISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFV 660
           GFVVW+ EHR+N DFRGPP HQI TS W+SF TMVFA RE ++SNLARFVVV+W FVV V
Sbjct: 601 GFVVWLFEHRVNTDFRGPPHHQIGTSFWFSFSTMVFAHREKVVSNLARFVVVVWCFVVLV 660

Query: 661 LTQSYTASLTSLLTVQELQPSITNINELLTKQSWVGYLEGSFVWGLLNSVG--IKNLKSY 720
           LTQSYTA+LTS LTVQ  QP+  N+ +L+    +VGY  G+FV   L   G  +  LK +
Sbjct: 661 LTQSYTANLTSFLTVQRFQPAAINVKDLIKNGDYVGYQHGAFVKDFLIKEGFNVSKLKPF 720

Query: 721 KCPEELDKLLKLGGSNGGIDAAFDETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGS 780
              EE   LL    SNG I AAFDE  YL+  LS++ +KY   +PT+KT GFGFAF   S
Sbjct: 721 GSSEECHALL----SNGSISAAFDEVAYLRAILSQYCSKYAIVEPTFKTAGFGFAFPRNS 780

Query: 781 PLVADISRAVLNVTESRKMNQLQMKWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTA 840
           PL  D+S+A+LNVT+  +M  ++ KWF  Q +       ++S+RL+L SFWGLFLI G A
Sbjct: 781 PLTGDVSKAILNVTQGDEMQHIENKWFMKQNDCPDPKTALSSNRLSLRSFWGLFLIAGIA 840

Query: 841 SIISLFIYFIIFLRKEQHTLHHTANEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPE 900
           S ++L I+  +FL + +HTL     + S  SI  K+ +L + +D++D+ S+TF+ S    
Sbjct: 841 SFLALLIFVFLFLYENRHTL----CDDSEDSIWRKLTSLFRNFDEKDIKSHTFKSSAVHH 900

Query: 901 VVDNKIQANHGNPVGTSPNSNYLLSPSNDSIHDTNH 913
           V              +SP + Y+ SPS   I    H
Sbjct: 901 V--------------SSPMTQYIPSPSTLQIAPRPH 905

BLAST of CmaCh12G006580 vs. ExPASy Swiss-Prot
Match: O04660 (Glutamate receptor 2.1 OS=Arabidopsis thaliana OX=3702 GN=GLR2.1 PE=2 SV=2)

HSP 1 Score: 782.3 bits (2019), Expect = 6.0e-225
Identity = 407/848 (48.00%), Postives = 554/848 (65.33%), Query Frame = 0

Query: 18  LLLVATAGEAQ----NVSVGVVLDMESWVGKVGLSCIDMSLSEFYEANPHYNTRVVLHRR 77
           ++ +   GEAQ    NV+VG+V D+ +    + L CI+MSLS+FY ++P   TR+V    
Sbjct: 16  IVFLMQVGEAQNRITNVNVGIVNDIGTAYSNMTLLCINMSLSDFYSSHPETQTRLVTTVV 75

Query: 78  DSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVPILTFTASSPSLAS 137
           DS  DVV AAAAALDLI N +V+AILGP TSMQ  F+I++   S VPI+T++A+SPSLAS
Sbjct: 76  DSKNDVVTAAAAALDLITNKEVKAILGPWTSMQAQFMIEMGQKSQVPIVTYSATSPSLAS 135

Query: 138 HRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILPYLIDALQAVNARV 197
            RS YFFR T+ DS+QV AI +++K + WR+V  +Y DD FG+GI+P L D LQ +N R+
Sbjct: 136 IRSQYFFRATYDDSSQVHAIKEIIKLFGWREVAPVYVDDTFGEGIMPRLTDVLQEINVRI 195

Query: 198 LYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKANEIGMMREGYVWIL 257
            YR+VI P  T+D+I  EL ++MT+PTRV +VH++  LA R FAKA EIG+M++GYVWIL
Sbjct: 196 PYRTVISPNATDDEISVELLRMMTLPTRVFVVHLVELLASRFFAKATEIGLMKQGYVWIL 255

Query: 258 TDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFVMENSVLNDPHLDI 317
           T+  T++L  M+ + +++M+G LGV+TYVP+S EL+ F+ +W + F + +       L++
Sbjct: 256 TNTITDVLSIMNETEIETMQGVLGVKTYVPRSKELENFRSRWTKRFPISD-------LNV 315

Query: 318 FGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNGEKIGEALSKTKFM 377
           +GLWAYDA  ALA+AIE+ G  N TF   +   N+++LQ LGVS+ G K+ + LS+ +F 
Sbjct: 316 YGLWAYDATTALALAIEEAGTSNLTFVKMDAKRNVSELQGLGVSQYGPKLLQTLSRVRFQ 375

Query: 378 GLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLL-------SSSETMT----- 437
           GL G+++ +NG+LQ + FEIVNVN  GG  +GFW  E GL        +S  T +     
Sbjct: 376 GLAGDFQFINGELQPSVFEIVNVNGQGGRTIGFWMKEYGLFKNVDQKPASKTTFSSWQDR 435

Query: 438 ---VIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMK------VKGTEVEGYCKDV 497
              +IWPG+TT+VPKGW+  T GKRL+IGVPV   + +F+K         T   G+  D 
Sbjct: 436 LRPIIWPGDTTSVPKGWEIPTNGKRLQIGVPVNNTFQQFVKATRDPITNSTIFSGFSIDY 495

Query: 498 FDAAIATLPYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVD 557
           F+A I  +PYD+ +D+IPF      + G YD L+ QVY G YD  V D TI +NRS YVD
Sbjct: 496 FEAVIQAIPYDISYDFIPF------QDGGYDALVYQVYLGKYDAVVADTTISSNRSMYVD 555

Query: 558 FTLPFTESGVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPD 617
           F+LP+T SGV ++VP + + +  + +FL PLTL LW+ SL  F  IG VVW+LEHR+NPD
Sbjct: 556 FSLPYTPSGVGLVVPVKDSVRRSSTIFLMPLTLALWLISLLSFFIIGLVVWVLEHRVNPD 615

Query: 618 FRGPPTHQISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLT 677
           F GP  +Q+ST  W+SF  MVFA RE ++S  AR VV+IW+F+V VLTQSYTASL SLLT
Sbjct: 616 FDGPGQYQLSTIFWFSFSIMVFAPRERVLSFWARVVVIIWYFLVLVLTQSYTASLASLLT 675

Query: 678 VQELQPSITNINELLTKQSWVGYLEGSFVWGLLNSVGIK--NLKSYKCPEELDKLLKLGG 737
            Q L P++TNIN LL K   VGY + SF+ G L   G    +L SY  PE  D LL  G 
Sbjct: 676 TQHLHPTVTNINSLLAKGESVGY-QSSFILGRLRDSGFSEASLVSYGSPEHCDALLSKGQ 735

Query: 738 SNGGIDAAFDETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVT 797
           + GG+ A   E PY+++FL ++ NKY      +K DG GF F IGSPLVADISRA+L V 
Sbjct: 736 AEGGVSAVLMEVPYVRIFLGQYCNKYKMVQTPFKVDGLGFVFPIGSPLVADISRAILKVE 795

Query: 798 ESRKMNQLQMKWF-------GDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFI 832
           ES K NQL+  WF        D   +    P V+  +L  DSFW LFL+      ++L  
Sbjct: 796 ESNKANQLENAWFKPIDESCPDPLTNPDPNPSVSFRQLGFDSFWVLFLVAAIVCTMALLK 849

BLAST of CmaCh12G006580 vs. ExPASy Swiss-Prot
Match: Q9SHV1 (Glutamate receptor 2.2 OS=Arabidopsis thaliana OX=3702 GN=GLR2.2 PE=2 SV=1)

HSP 1 Score: 761.9 bits (1966), Expect = 8.4e-219
Identity = 399/868 (45.97%), Postives = 561/868 (64.63%), Query Frame = 0

Query: 30  VSVGVVLDMESWVGKVGLSCIDMSLSEFYEANPHYNTRVVLHRRDSAGDVVGAAAAALDL 89
           V++GVV D+ +    V + CI+MSL++FY + P + TR+V++  DS  DVVGAA AA+DL
Sbjct: 32  VNIGVVSDVGTSYPDVAMLCINMSLADFYSSRPQFQTRLVVNVGDSKNDVVGAATAAIDL 91

Query: 90  IKNHKVEAILGPTTSMQTNFVIKLAHNSHVPILTFTASSPSLASHRSPYFFRLTHTDSAQ 149
           IKN +V+AILGP TSMQ +F+I++   S VP+++++A+SPSL S RSPYFFR T+ DS+Q
Sbjct: 92  IKNKQVKAILGPWTSMQAHFLIEIGQKSRVPVVSYSATSPSLTSLRSPYFFRATYEDSSQ 151

Query: 150 VAAISDLVKFYNWRQVVLIYQDDEFGDGILPYLIDALQAVNARVLYRSVIDPTPTEDQIE 209
           V AI  ++K + WR+VV +Y D+ FG+GI+P L D+LQ +N R+ YRSVI    T+  I 
Sbjct: 152 VHAIKAIIKLFGWREVVPVYIDNTFGEGIMPRLTDSLQDINVRIPYRSVIPLNATDQDIS 211

Query: 210 EELYKLMTMPTRVLLVHMLPSLAIRLFAKANEIGMMREGYVWILTDATTNLLGSMSSSVL 269
            EL K+M MPTRV +VHM  SLA  +F KA E+G+M+ GYVWILT+   + L S++ + +
Sbjct: 212 VELLKMMNMPTRVFIVHMSSSLASTVFIKAKELGLMKPGYVWILTNGVMDGLRSINETGI 271

Query: 270 KSMEGALGVRTYVPKSMELDRFKIKWKREFVMENSVLNDPHLDIFGLWAYDAARALAIAI 329
           ++MEG LG++TY+PKS +L+ F+ +WKR F           L+++GLWAYDA  ALA+AI
Sbjct: 272 EAMEGVLGIKTYIPKSKDLETFRSRWKRRF-------PQMELNVYGLWAYDATTALAMAI 331

Query: 330 EKTGVKNFTFESPNRSENLTDLQTLGVSRNGEKIGEALSKTKFMGLTGNYEIVNGQLQSA 389
           E  G+ N TF + +  +N+++L  LG+S+ G K+ + +S  +F GL G++  V+GQLQ +
Sbjct: 332 EDAGINNMTFSNVDTGKNVSELDGLGLSQFGPKLLQTVSTVQFKGLAGDFHFVSGQLQPS 391

Query: 390 AFEIVNVNSNGGNRVGFWNPEKGLLS-------SSETMT--------VIWPGNTTAVPKG 449
            FEIVN+   G   +GFW    GL+        S  T++        +IWPG   +VPKG
Sbjct: 392 VFEIVNMIGTGERSIGFWTEGNGLVKKLDQEPRSIGTLSTWPDHLKHIIWPGEAVSVPKG 451

Query: 450 WDFQTTGKRLRIGVPVKKGYIEFMKV------KGTEVEGYCKDVFDAAIATLPYDVPFDY 509
           W+  T GK+LRIGVP + G+ + +KV        T V+G+C D F+A I  +PYDV +++
Sbjct: 452 WEIPTNGKKLRIGVPKRIGFTDLVKVTRDPITNSTVVKGFCIDFFEAVIQAMPYDVSYEF 511

Query: 510 IPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTESGVSMIVPT 569
            PF   NG  AG++++L+ QVY G +D  VGD TI+ANRS +VDFTLPF +SGV +IVP 
Sbjct: 512 FPFEKPNGEPAGNHNDLVHQVYLGQFDAVVGDTTILANRSSFVDFTLPFMKSGVGLIVPL 571

Query: 570 QANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQISTSLWYS 629
           +   K   + FLKPL+++LW+T+L FF  +G  VW LEHR+N DFRGP  +Q ST  W++
Sbjct: 572 KDEVKRDKFSFLKPLSIELWLTTLVFFFLVGISVWTLEHRVNSDFRGPANYQASTIFWFA 631

Query: 630 FCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSITNINELLT 689
           F TMVFA RE ++S  AR +VV W+FV+ VLTQSYTASL SLLT Q+L P+IT+++ LL 
Sbjct: 632 FSTMVFAPRERVLSFGARSLVVTWYFVLLVLTQSYTASLASLLTSQQLNPTITSMSSLLH 691

Query: 690 KQSWVGYLEGSFVWGLLNSVGI--KNLKSYKCPEELDKLLKLGGSNGGIDAAFDETPYLK 749
           +   VGY   SF+ G LN  G    +L  +   EE D+LLK G  NGG+ AAF  TPY++
Sbjct: 692 RGETVGYQRTSFILGKLNETGFPQSSLVPFDTAEECDELLKKGPKNGGVAAAFLGTPYVR 751

Query: 750 LFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQMKWFGDQ 809
           LFL ++ N Y   +  +  DGFGF F IGSPLVAD+SRA+L V ES K  +L+  WF  +
Sbjct: 752 LFLGQYCNTYKMVEEPFNVDGFGFVFPIGSPLVADVSRAILKVAESPKAVELEHAWFKKK 811

Query: 810 GNSW-------SSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHT 868
             S         S P VT+ +L + SFW LFL+     +++L  +   FL K        
Sbjct: 812 EQSCPDPVTNPDSNPTVTAIQLGVGSFWFLFLVVFVVCVLALGKFTFCFLWK-------- 871

BLAST of CmaCh12G006580 vs. ExPASy TrEMBL
Match: A0A6J1KRF9 (Glutamate receptor OS=Cucurbita maxima OX=3661 GN=LOC111496544 PE=3 SV=1)

HSP 1 Score: 1878.6 bits (4865), Expect = 0.0e+00
Identity = 941/941 (100.00%), Postives = 941/941 (100.00%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60
           MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA
Sbjct: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60

Query: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120
           NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP
Sbjct: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120

Query: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180
           ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP
Sbjct: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180

Query: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240
           YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN
Sbjct: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240

Query: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300
           EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV
Sbjct: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300

Query: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360
           MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG
Sbjct: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360

Query: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420
           EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM
Sbjct: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420

Query: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480
           TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL
Sbjct: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480

Query: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540
           PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES
Sbjct: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540

Query: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600
           GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ
Sbjct: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600

Query: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660
           ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI
Sbjct: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660

Query: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720
           TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD
Sbjct: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720

Query: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780
           ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM
Sbjct: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780

Query: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840
           KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA
Sbjct: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840

Query: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900
           NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL
Sbjct: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900

Query: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIVHRS 942
           SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIVHRS
Sbjct: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIVHRS 941

BLAST of CmaCh12G006580 vs. ExPASy TrEMBL
Match: A0A6J1GKC9 (Glutamate receptor OS=Cucurbita moschata OX=3662 GN=LOC111454709 PE=3 SV=1)

HSP 1 Score: 1709.5 bits (4426), Expect = 0.0e+00
Identity = 855/941 (90.86%), Postives = 896/941 (95.22%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60
           M PNQGSRRAL FIA C+LLVATAGEA NVSVGVVLDM+SW+GKVGLSCI MSLSEFYEA
Sbjct: 1   MNPNQGSRRALCFIAACVLLVATAGEAHNVSVGVVLDMDSWIGKVGLSCIHMSLSEFYEA 60

Query: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120
           NPHYNTR+VLH  DSAGDVV AAAAA+DLIKN+KVEAILGPTTSMQTNFVIKL H +HVP
Sbjct: 61  NPHYNTRIVLHPNDSAGDVVAAAAAAVDLIKNNKVEAILGPTTSMQTNFVIKLGHKAHVP 120

Query: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180
           +LTFTASSP+LAS+RSPYFFRLTHTDSA+VAAISDLVKFYNWRQ+VLIYQDDEFGDG+LP
Sbjct: 121 VLTFTASSPALASNRSPYFFRLTHTDSARVAAISDLVKFYNWRQIVLIYQDDEFGDGMLP 180

Query: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240
           YLIDALQ VNARV YRS+I PT TEDQI EEL+KL+TMPTRV LVHM PSLAIRLFAKAN
Sbjct: 181 YLIDALQGVNARVPYRSLIVPTTTEDQIGEELHKLITMPTRVFLVHMQPSLAIRLFAKAN 240

Query: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300
           EIGMMREGYVWILTDAT NLL SMSSSVLKSMEGALGVRTYVPKSMEL RFKIKWKR+F+
Sbjct: 241 EIGMMREGYVWILTDATANLLDSMSSSVLKSMEGALGVRTYVPKSMELKRFKIKWKRKFI 300

Query: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360
           MEN VLNDPHLDIFGLWAYDAARALA+AIEKTGVKNFTFE+ N SENLTDLQTLGVSRNG
Sbjct: 301 MENFVLNDPHLDIFGLWAYDAARALAMAIEKTGVKNFTFENTNGSENLTDLQTLGVSRNG 360

Query: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420
           EKIGEALSKTKFMGLTGNYEIVNGQLQSA FEIVNVNSNGGNRVGFWN EKGLLS++  M
Sbjct: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAEFEIVNVNSNGGNRVGFWNHEKGLLSNN--M 420

Query: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480
           TVIWPGNT AVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL
Sbjct: 421 TVIWPGNTAAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480

Query: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540
           PYDVPFDYIPFAL NGSRAGSYD+LIMQVY+GVYDGAVGD+TI+ANRSKYVDFTLPFT+S
Sbjct: 481 PYDVPFDYIPFALPNGSRAGSYDDLIMQVYEGVYDGAVGDITIIANRSKYVDFTLPFTDS 540

Query: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600
           GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVF+GFVVWILEHRINPDFRGPP+HQ
Sbjct: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFMGFVVWILEHRINPDFRGPPSHQ 600

Query: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660
           I TSLW+SFCTMVFAQRETLISNLAR VVVIWFFVVFVLTQSYTASL+SLLTVQ+LQP+I
Sbjct: 601 IGTSLWFSFCTMVFAQRETLISNLARLVVVIWFFVVFVLTQSYTASLSSLLTVQQLQPTI 660

Query: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720
           TNINELLTKQS VGYLEGSFVWGLL SVGIKNLKSY+ PEELDKLLKLGGSNGGIDAAFD
Sbjct: 661 TNINELLTKQSSVGYLEGSFVWGLLTSVGIKNLKSYRSPEELDKLLKLGGSNGGIDAAFD 720

Query: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780
           ETPY+KLFLSKFPNKYI ADPTYKTDGFGFAFSIGSPLV DISRAVLNVTES++MNQLQM
Sbjct: 721 ETPYIKLFLSKFPNKYIRADPTYKTDGFGFAFSIGSPLVVDISRAVLNVTESKRMNQLQM 780

Query: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840
           KWFGD+GNSWSSIPKVTSS LNL SFWGLFLITG ASIISL IYFIIFL KEQHTL HTA
Sbjct: 781 KWFGDRGNSWSSIPKVTSSGLNLGSFWGLFLITGIASIISLLIYFIIFLHKEQHTLRHTA 840

Query: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900
           NE SNSSI+SKIRALLKIYDKRDLTSYTFRKSNPP+VVDNKIQANHGNPVGTSPNSNYLL
Sbjct: 841 NESSNSSIKSKIRALLKIYDKRDLTSYTFRKSNPPQVVDNKIQANHGNPVGTSPNSNYLL 900

Query: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIVHRS 942
           SPSNDSIHDTNHEF RSEDLNPSNQAVEMVVHTTMEIV +S
Sbjct: 901 SPSNDSIHDTNHEFSRSEDLNPSNQAVEMVVHTTMEIVPQS 939

BLAST of CmaCh12G006580 vs. ExPASy TrEMBL
Match: A0A6J1GJ12 (Glutamate receptor OS=Cucurbita moschata OX=3662 GN=LOC111454707 PE=3 SV=1)

HSP 1 Score: 1634.4 bits (4231), Expect = 0.0e+00
Identity = 818/938 (87.21%), Postives = 871/938 (92.86%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60
           MKPN GS RALWFIAGC+LLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSE YEA
Sbjct: 1   MKPNHGSHRALWFIAGCMLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSELYEA 60

Query: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120
           NPHY TRVVLHRRDSAGDVVGAAAAA+DLI N+KVEAILGPTTSMQTNFVIKLAH +HVP
Sbjct: 61  NPHYKTRVVLHRRDSAGDVVGAAAAAVDLINNNKVEAILGPTTSMQTNFVIKLAHKAHVP 120

Query: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180
           ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAI DL+KFYNWRQVVLIYQDDEFGDG+LP
Sbjct: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAIRDLLKFYNWRQVVLIYQDDEFGDGMLP 180

Query: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240
           YLIDALQ VNARV Y+S+IDPT TEDQIEEELYKLMTMPTRV LVHMLPSLAIRLFAKAN
Sbjct: 181 YLIDALQGVNARVPYQSIIDPTATEDQIEEELYKLMTMPTRVFLVHMLPSLAIRLFAKAN 240

Query: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300
           +IGMMREGYVWILTDATTNLL SM SSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV
Sbjct: 241 QIGMMREGYVWILTDATTNLLDSMPSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300

Query: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360
           MENSV NDP+LDIFGLWAYDAARALAIA+EKTG KNFTFE+PNR +NLTDL+TLGVSRNG
Sbjct: 301 MENSVPNDPYLDIFGLWAYDAARALAIAVEKTGAKNFTFENPNRGQNLTDLETLGVSRNG 360

Query: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420
           EKI EALSKTKFMGLTGNYEIVNGQLQSA FEIVNVNSNGGNRVGFWNPEKGLLS++  M
Sbjct: 361 EKIVEALSKTKFMGLTGNYEIVNGQLQSAEFEIVNVNSNGGNRVGFWNPEKGLLSNN--M 420

Query: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480
           TVIWPGNT AVPKGW+F T GKRLRIGVPVK+GY EF++V G +VEGYC+DVFDA I  L
Sbjct: 421 TVIWPGNTAAVPKGWEFPTAGKRLRIGVPVKEGYSEFVRVNGKKVEGYCRDVFDAVIEAL 480

Query: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540
           PY +PFDYIPFAL NGSRAGSYD+LIMQV  GVYDGA+GD+TIVANRSKYVDFTLPFT+S
Sbjct: 481 PYALPFDYIPFALPNGSRAGSYDDLIMQVNTGVYDGAIGDITIVANRSKYVDFTLPFTDS 540

Query: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600
           GVSM+VPT+ANS NRAWLFLKPLT+ LWITSLCFFVF+GFVVWILEHRINP FRGPP+H 
Sbjct: 541 GVSMVVPTKANSMNRAWLFLKPLTVGLWITSLCFFVFMGFVVWILEHRINPAFRGPPSHH 600

Query: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660
           I TSLWYSFCTMVFAQRETLISNLARFVVVIWFFV+FVLTQSYTASLTSLLTVQ+LQP+I
Sbjct: 601 IGTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVMFVLTQSYTASLTSLLTVQQLQPTI 660

Query: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720
            NINELLTKQSWVGYL+GSFVWGLL SVGIKNLKSY+ PEELDKLLKLGGSNGGIDAAFD
Sbjct: 661 ININELLTKQSWVGYLDGSFVWGLLTSVGIKNLKSYRSPEELDKLLKLGGSNGGIDAAFD 720

Query: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780
           ETPY+KLFLSKFPNKYITA+PTYKTDGFGFAFSI SPLVADISRAVLNV ES KMNQLQM
Sbjct: 721 ETPYIKLFLSKFPNKYITANPTYKTDGFGFAFSIDSPLVADISRAVLNVRESEKMNQLQM 780

Query: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840
           KWFG+  NSWSS+ KVTSSRL+L +FWGLFLI G A+IISLFIYF IFLRKE HTL +T 
Sbjct: 781 KWFGED-NSWSSV-KVTSSRLSLSNFWGLFLIVGIAAIISLFIYFFIFLRKELHTLRNTT 840

Query: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900
            E SNSSIRSKIRALL+IYD RDLTS+TFRKSNPP+VVDNKIQANHG+ VG S +SNYLL
Sbjct: 841 TEASNSSIRSKIRALLRIYDNRDLTSHTFRKSNPPQVVDNKIQANHGDSVGASSSSNYLL 900

Query: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIV 939
           S SNDSIHDTNH F +S DL+PSNQ VE V+HTT EIV
Sbjct: 901 SSSNDSIHDTNHGFSKSGDLSPSNQDVETVIHTTNEIV 934

BLAST of CmaCh12G006580 vs. ExPASy TrEMBL
Match: A0A6J1GJ59 (Glutamate receptor OS=Cucurbita moschata OX=3662 GN=LOC111454707 PE=3 SV=1)

HSP 1 Score: 1567.0 bits (4056), Expect = 0.0e+00
Identity = 781/886 (88.15%), Postives = 829/886 (93.57%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60
           MKPN GS RALWFIAGC+LLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSE YEA
Sbjct: 1   MKPNHGSHRALWFIAGCMLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSELYEA 60

Query: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120
           NPHY TRVVLHRRDSAGDVVGAAAAA+DLI N+KVEAILGPTTSMQTNFVIKLAH +HVP
Sbjct: 61  NPHYKTRVVLHRRDSAGDVVGAAAAAVDLINNNKVEAILGPTTSMQTNFVIKLAHKAHVP 120

Query: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180
           ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAI DL+KFYNWRQVVLIYQDDEFGDG+LP
Sbjct: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAIRDLLKFYNWRQVVLIYQDDEFGDGMLP 180

Query: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240
           YLIDALQ VNARV Y+S+IDPT TEDQIEEELYKLMTMPTRV LVHMLPSLAIRLFAKAN
Sbjct: 181 YLIDALQGVNARVPYQSIIDPTATEDQIEEELYKLMTMPTRVFLVHMLPSLAIRLFAKAN 240

Query: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300
           +IGMMREGYVWILTDATTNLL SM SSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV
Sbjct: 241 QIGMMREGYVWILTDATTNLLDSMPSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300

Query: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360
           MENSV NDP+LDIFGLWAYDAARALAIA+EKTG KNFTFE+PNR +NLTDL+TLGVSRNG
Sbjct: 301 MENSVPNDPYLDIFGLWAYDAARALAIAVEKTGAKNFTFENPNRGQNLTDLETLGVSRNG 360

Query: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420
           EKI EALSKTKFMGLTGNYEIVNGQLQSA FEIVNVNSNGGNRVGFWNPEKGLLS++  M
Sbjct: 361 EKIVEALSKTKFMGLTGNYEIVNGQLQSAEFEIVNVNSNGGNRVGFWNPEKGLLSNN--M 420

Query: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480
           TVIWPGNT AVPKGW+F T GKRLRIGVPVK+GY EF++V G +VEGYC+DVFDA I  L
Sbjct: 421 TVIWPGNTAAVPKGWEFPTAGKRLRIGVPVKEGYSEFVRVNGKKVEGYCRDVFDAVIEAL 480

Query: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540
           PY +PFDYIPFAL NGSRAGSYD+LIMQV  GVYDGA+GD+TIVANRSKYVDFTLPFT+S
Sbjct: 481 PYALPFDYIPFALPNGSRAGSYDDLIMQVNTGVYDGAIGDITIVANRSKYVDFTLPFTDS 540

Query: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600
           GVSM+VPT+ANS NRAWLFLKPLT+ LWITSLCFFVF+GFVVWILEHRINP FRGPP+H 
Sbjct: 541 GVSMVVPTKANSMNRAWLFLKPLTVGLWITSLCFFVFMGFVVWILEHRINPAFRGPPSHH 600

Query: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660
           I TSLWYSFCTMVFAQRETLISNLARFVVVIWFFV+FVLTQSYTASLTSLLTVQ+LQP+I
Sbjct: 601 IGTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVMFVLTQSYTASLTSLLTVQQLQPTI 660

Query: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720
            NINELLTKQSWVGYL+GSFVWGLL SVGIKNLKSY+ PEELDKLLKLGGSNGGIDAAFD
Sbjct: 661 ININELLTKQSWVGYLDGSFVWGLLTSVGIKNLKSYRSPEELDKLLKLGGSNGGIDAAFD 720

Query: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780
           ETPY+KLFLSKFPNKYITA+PTYKTDGFGFAFSI SPLVADISRAVLNV ES KMNQLQM
Sbjct: 721 ETPYIKLFLSKFPNKYITANPTYKTDGFGFAFSIDSPLVADISRAVLNVRESEKMNQLQM 780

Query: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840
           KWFG+  NSWSS+ KVTSSRL+L +FWGLFLI G A+IISLFIYF IFLRKE HTL +T 
Sbjct: 781 KWFGED-NSWSSV-KVTSSRLSLSNFWGLFLIVGIAAIISLFIYFFIFLRKELHTLRNTT 840

Query: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANH 887
            E SNSSIRSKIRALL+IYD RDLTS+TFRKSNPP+VVDNKIQANH
Sbjct: 841 TEASNSSIRSKIRALLRIYDNRDLTSHTFRKSNPPQVVDNKIQANH 882

BLAST of CmaCh12G006580 vs. ExPASy TrEMBL
Match: A0A6J1KUX1 (Glutamate receptor OS=Cucurbita maxima OX=3661 GN=LOC111496637 PE=3 SV=1)

HSP 1 Score: 1541.9 bits (3991), Expect = 0.0e+00
Identity = 765/937 (81.64%), Postives = 838/937 (89.43%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60
           M  NQG+ RALWFIAGCLL VATAGEA+NVSVGVVLDMESWVGK+GLSCI MSLSEFY+A
Sbjct: 1   MNTNQGTHRALWFIAGCLLFVATAGEARNVSVGVVLDMESWVGKMGLSCIHMSLSEFYQA 60

Query: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120
           NPHYNTR+VLH +DSAGDVVGAAAAALDLI NHKVEAILGPTTSMQTNFVIKLAH +HVP
Sbjct: 61  NPHYNTRIVLHPKDSAGDVVGAAAAALDLINNHKVEAILGPTTSMQTNFVIKLAHKAHVP 120

Query: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180
           ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAI+ LVK YNWR++VLIYQDDEFGDG+ P
Sbjct: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAITALVKAYNWRELVLIYQDDEFGDGMSP 180

Query: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240
           Y I ALQ VNARV Y+S+IDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLF+KAN
Sbjct: 181 YFIHALQGVNARVPYQSIIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFSKAN 240

Query: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300
           EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKR+F+
Sbjct: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKRKFI 300

Query: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360
           +ENSV NDP+LDIFGLWAYDAARALA+A+EKTG KNFTF++PN SENLTDL+TLGVSRNG
Sbjct: 301 VENSVPNDPYLDIFGLWAYDAARALAMAVEKTGAKNFTFQNPNGSENLTDLETLGVSRNG 360

Query: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420
           EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLS++  M
Sbjct: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSNN--M 420

Query: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480
           TV+WPGNT AVPKGW+F T GKRLRIGVPVK+GY EF+K       GYC DVF+A IATL
Sbjct: 421 TVVWPGNTAAVPKGWEFPTAGKRLRIGVPVKEGYSEFVK-------GYCTDVFNAVIATL 480

Query: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540
           PYDVPFDYIPFAL NGS AGSYD+LIMQV+KGVYDGAVGD+TI+ANRSKYVDFTLPFTE 
Sbjct: 481 PYDVPFDYIPFALPNGSSAGSYDDLIMQVHKGVYDGAVGDITIIANRSKYVDFTLPFTEP 540

Query: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600
           GVSM+VPTQ NSKN+AWLFLKPLTLDLWITSLCFFVF GFVVWILEHRIN DFRGPP+H 
Sbjct: 541 GVSMVVPTQPNSKNKAWLFLKPLTLDLWITSLCFFVFTGFVVWILEHRINQDFRGPPSHH 600

Query: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660
           I TSLW+SFCTMVFAQRETLISNLARFVV+IWFFVVF+LT+SY  SLTSLLT+Q+LQP+I
Sbjct: 601 IGTSLWFSFCTMVFAQRETLISNLARFVVIIWFFVVFILTESYMGSLTSLLTLQQLQPTI 660

Query: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720
           TNIN+LL KQS VGY  GSFVW LL S+G+KNLK Y   EELDKL KLG SN GID AFD
Sbjct: 661 TNINQLLKKQSSVGYQHGSFVWELLTSLGLKNLKPYGSLEELDKLFKLGSSNDGIDVAFD 720

Query: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780
           E PY+KLF  KFPNKY   DP YKTDGFGFAF IGS LVADISRA+L+VTES K+NQL  
Sbjct: 721 EIPYIKLFRRKFPNKYTMVDPNYKTDGFGFAFPIGSLLVADISRAILHVTESEKINQLHK 780

Query: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840
           +WF DQ NSWSSIPKVTSS L++++FWGLFLI G  +I  L +YF+IFL KE+HTL  TA
Sbjct: 781 EWFSDQANSWSSIPKVTSSELSMNNFWGLFLIVGVVAITVLLVYFVIFLYKEKHTLGRTA 840

Query: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900
           NEGSN SI +KIRALLKIYD++DLTS+TF+KSNPP+VVDNKI   H + VG SPNSNY  
Sbjct: 841 NEGSNFSIGNKIRALLKIYDQKDLTSHTFKKSNPPKVVDNKIYPIHDDSVGISPNSNYPS 900

Query: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEI 938
           SPSN S+HDT +E F S DL+P+NQ VEMV+HT  E+
Sbjct: 901 SPSNYSVHDTIYESFESGDLSPNNQIVEMVIHTITEV 928

BLAST of CmaCh12G006580 vs. NCBI nr
Match: XP_023002784.1 (glutamate receptor 2.1-like [Cucurbita maxima])

HSP 1 Score: 1878.6 bits (4865), Expect = 0.0e+00
Identity = 941/941 (100.00%), Postives = 941/941 (100.00%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60
           MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA
Sbjct: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60

Query: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120
           NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP
Sbjct: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120

Query: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180
           ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP
Sbjct: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180

Query: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240
           YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN
Sbjct: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240

Query: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300
           EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV
Sbjct: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300

Query: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360
           MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG
Sbjct: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360

Query: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420
           EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM
Sbjct: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420

Query: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480
           TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL
Sbjct: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480

Query: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540
           PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES
Sbjct: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540

Query: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600
           GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ
Sbjct: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600

Query: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660
           ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI
Sbjct: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660

Query: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720
           TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD
Sbjct: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720

Query: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780
           ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM
Sbjct: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780

Query: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840
           KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA
Sbjct: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840

Query: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900
           NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL
Sbjct: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900

Query: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIVHRS 942
           SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIVHRS
Sbjct: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIVHRS 941

BLAST of CmaCh12G006580 vs. NCBI nr
Match: XP_022951994.1 (glutamate receptor 2.1-like [Cucurbita moschata])

HSP 1 Score: 1709.5 bits (4426), Expect = 0.0e+00
Identity = 855/941 (90.86%), Postives = 896/941 (95.22%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60
           M PNQGSRRAL FIA C+LLVATAGEA NVSVGVVLDM+SW+GKVGLSCI MSLSEFYEA
Sbjct: 1   MNPNQGSRRALCFIAACVLLVATAGEAHNVSVGVVLDMDSWIGKVGLSCIHMSLSEFYEA 60

Query: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120
           NPHYNTR+VLH  DSAGDVV AAAAA+DLIKN+KVEAILGPTTSMQTNFVIKL H +HVP
Sbjct: 61  NPHYNTRIVLHPNDSAGDVVAAAAAAVDLIKNNKVEAILGPTTSMQTNFVIKLGHKAHVP 120

Query: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180
           +LTFTASSP+LAS+RSPYFFRLTHTDSA+VAAISDLVKFYNWRQ+VLIYQDDEFGDG+LP
Sbjct: 121 VLTFTASSPALASNRSPYFFRLTHTDSARVAAISDLVKFYNWRQIVLIYQDDEFGDGMLP 180

Query: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240
           YLIDALQ VNARV YRS+I PT TEDQI EEL+KL+TMPTRV LVHM PSLAIRLFAKAN
Sbjct: 181 YLIDALQGVNARVPYRSLIVPTTTEDQIGEELHKLITMPTRVFLVHMQPSLAIRLFAKAN 240

Query: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300
           EIGMMREGYVWILTDAT NLL SMSSSVLKSMEGALGVRTYVPKSMEL RFKIKWKR+F+
Sbjct: 241 EIGMMREGYVWILTDATANLLDSMSSSVLKSMEGALGVRTYVPKSMELKRFKIKWKRKFI 300

Query: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360
           MEN VLNDPHLDIFGLWAYDAARALA+AIEKTGVKNFTFE+ N SENLTDLQTLGVSRNG
Sbjct: 301 MENFVLNDPHLDIFGLWAYDAARALAMAIEKTGVKNFTFENTNGSENLTDLQTLGVSRNG 360

Query: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420
           EKIGEALSKTKFMGLTGNYEIVNGQLQSA FEIVNVNSNGGNRVGFWN EKGLLS++  M
Sbjct: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAEFEIVNVNSNGGNRVGFWNHEKGLLSNN--M 420

Query: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480
           TVIWPGNT AVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL
Sbjct: 421 TVIWPGNTAAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480

Query: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540
           PYDVPFDYIPFAL NGSRAGSYD+LIMQVY+GVYDGAVGD+TI+ANRSKYVDFTLPFT+S
Sbjct: 481 PYDVPFDYIPFALPNGSRAGSYDDLIMQVYEGVYDGAVGDITIIANRSKYVDFTLPFTDS 540

Query: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600
           GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVF+GFVVWILEHRINPDFRGPP+HQ
Sbjct: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFMGFVVWILEHRINPDFRGPPSHQ 600

Query: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660
           I TSLW+SFCTMVFAQRETLISNLAR VVVIWFFVVFVLTQSYTASL+SLLTVQ+LQP+I
Sbjct: 601 IGTSLWFSFCTMVFAQRETLISNLARLVVVIWFFVVFVLTQSYTASLSSLLTVQQLQPTI 660

Query: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720
           TNINELLTKQS VGYLEGSFVWGLL SVGIKNLKSY+ PEELDKLLKLGGSNGGIDAAFD
Sbjct: 661 TNINELLTKQSSVGYLEGSFVWGLLTSVGIKNLKSYRSPEELDKLLKLGGSNGGIDAAFD 720

Query: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780
           ETPY+KLFLSKFPNKYI ADPTYKTDGFGFAFSIGSPLV DISRAVLNVTES++MNQLQM
Sbjct: 721 ETPYIKLFLSKFPNKYIRADPTYKTDGFGFAFSIGSPLVVDISRAVLNVTESKRMNQLQM 780

Query: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840
           KWFGD+GNSWSSIPKVTSS LNL SFWGLFLITG ASIISL IYFIIFL KEQHTL HTA
Sbjct: 781 KWFGDRGNSWSSIPKVTSSGLNLGSFWGLFLITGIASIISLLIYFIIFLHKEQHTLRHTA 840

Query: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900
           NE SNSSI+SKIRALLKIYDKRDLTSYTFRKSNPP+VVDNKIQANHGNPVGTSPNSNYLL
Sbjct: 841 NESSNSSIKSKIRALLKIYDKRDLTSYTFRKSNPPQVVDNKIQANHGNPVGTSPNSNYLL 900

Query: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIVHRS 942
           SPSNDSIHDTNHEF RSEDLNPSNQAVEMVVHTTMEIV +S
Sbjct: 901 SPSNDSIHDTNHEFSRSEDLNPSNQAVEMVVHTTMEIVPQS 939

BLAST of CmaCh12G006580 vs. NCBI nr
Match: XP_023536843.1 (glutamate receptor 2.8-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1697.6 bits (4395), Expect = 0.0e+00
Identity = 850/941 (90.33%), Postives = 889/941 (94.47%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60
           M PNQGSRRALWFIAGC+LLVATAGEAQNVSVGVVLDMESW+GKVGLSCI MSLSEFYEA
Sbjct: 1   MNPNQGSRRALWFIAGCVLLVATAGEAQNVSVGVVLDMESWIGKVGLSCIHMSLSEFYEA 60

Query: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120
           NPHY TR+VLH +DSAGD V AAAAA+DLIKN+KVEAILGPTTSMQTNFVIKLAH +HVP
Sbjct: 61  NPHYKTRIVLHPKDSAGDAVAAAAAAVDLIKNNKVEAILGPTTSMQTNFVIKLAHKAHVP 120

Query: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180
           +LTFTASSPSLASHRSPYFFRLTHTDSAQ+AAIS LVK YNWRQVVLIYQDDEFGDG+ P
Sbjct: 121 VLTFTASSPSLASHRSPYFFRLTHTDSAQLAAISALVKAYNWRQVVLIYQDDEFGDGMSP 180

Query: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240
           YLI ALQ VNARV Y+S+IDPT TED I EEL+KL+TMPTRV LVHM PSLAIRLFAKAN
Sbjct: 181 YLIHALQGVNARVPYQSIIDPTATEDHIGEELHKLITMPTRVFLVHMQPSLAIRLFAKAN 240

Query: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300
           EI MMREGYVWILTDAT NLL SMSSSVLKSMEGALGVRTYVPKSMEL+RFK+KWKR+F+
Sbjct: 241 EIRMMREGYVWILTDATANLLDSMSSSVLKSMEGALGVRTYVPKSMELERFKLKWKRKFI 300

Query: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360
           MEN VLNDPHLDIFGLWAYDAARALA+AIEKTGVKNFTFE+ N SENLTDL+TLGVSRNG
Sbjct: 301 MENFVLNDPHLDIFGLWAYDAARALAMAIEKTGVKNFTFENQNGSENLTDLRTLGVSRNG 360

Query: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420
           EKI EALSKTKF+GLTGNYEIVNGQLQSA FEIVNVNSNGGNRVGF NPEKGLLS SETM
Sbjct: 361 EKIREALSKTKFIGLTGNYEIVNGQLQSAGFEIVNVNSNGGNRVGFCNPEKGLLSLSETM 420

Query: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480
           TVIWPGNT AVPKGWDFQTTGKRLRIGVPVKKGYIEFMKV GTEVEGYCKDVFDAAIATL
Sbjct: 421 TVIWPGNTAAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVNGTEVEGYCKDVFDAAIATL 480

Query: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540
           PYDVPFDYIPFAL NGSRAGSYD+LIMQV  GVYDGAVGD+TIVANRSKYVDFTLPFT+S
Sbjct: 481 PYDVPFDYIPFALPNGSRAGSYDDLIMQVNTGVYDGAVGDITIVANRSKYVDFTLPFTDS 540

Query: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600
           GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVF+GFVVWILEHRINPDFRGPP+HQ
Sbjct: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFMGFVVWILEHRINPDFRGPPSHQ 600

Query: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660
           I TSLW+SFCTMVFAQRETLISNLAR VVVIWFFVVFVLTQSYTASL+SLLTVQ+LQP+I
Sbjct: 601 IGTSLWFSFCTMVFAQRETLISNLARLVVVIWFFVVFVLTQSYTASLSSLLTVQQLQPTI 660

Query: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720
           TNINELLTKQS VGYLEGSFVWGLL SVGIKNLKSY+ PEELD LLKLGGSNGGIDAAFD
Sbjct: 661 TNINELLTKQSSVGYLEGSFVWGLLTSVGIKNLKSYRSPEELDTLLKLGGSNGGIDAAFD 720

Query: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780
           ETPY+KLFLSKFPNKYITADPTYKTDGFGFAF IGSPLVADISRA+LNV ES+KMNQLQM
Sbjct: 721 ETPYIKLFLSKFPNKYITADPTYKTDGFGFAFPIGSPLVADISRAMLNVIESKKMNQLQM 780

Query: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840
           KWFGDQGNSWSSIPKVTSS LNL SFWGLFLITGTASIISL IYFIIFL KEQHTL HTA
Sbjct: 781 KWFGDQGNSWSSIPKVTSSGLNLGSFWGLFLITGTASIISLLIYFIIFLHKEQHTLRHTA 840

Query: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900
           NE SNSSI+SKIRALLKIYDKRDLTSYTFRKSNPPEV+DNKIQANHGNPVGTSPNSNYLL
Sbjct: 841 NESSNSSIKSKIRALLKIYDKRDLTSYTFRKSNPPEVIDNKIQANHGNPVGTSPNSNYLL 900

Query: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIVHRS 942
           SP NDSI+DTNHEF RSEDLN SNQ VEMVVHTTMEIV +S
Sbjct: 901 SPLNDSIYDTNHEFSRSEDLNLSNQTVEMVVHTTMEIVPQS 941

BLAST of CmaCh12G006580 vs. NCBI nr
Match: XP_022951991.1 (glutamate receptor 2.1-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 1634.4 bits (4231), Expect = 0.0e+00
Identity = 818/938 (87.21%), Postives = 871/938 (92.86%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60
           MKPN GS RALWFIAGC+LLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSE YEA
Sbjct: 1   MKPNHGSHRALWFIAGCMLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSELYEA 60

Query: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120
           NPHY TRVVLHRRDSAGDVVGAAAAA+DLI N+KVEAILGPTTSMQTNFVIKLAH +HVP
Sbjct: 61  NPHYKTRVVLHRRDSAGDVVGAAAAAVDLINNNKVEAILGPTTSMQTNFVIKLAHKAHVP 120

Query: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180
           ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAI DL+KFYNWRQVVLIYQDDEFGDG+LP
Sbjct: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAIRDLLKFYNWRQVVLIYQDDEFGDGMLP 180

Query: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240
           YLIDALQ VNARV Y+S+IDPT TEDQIEEELYKLMTMPTRV LVHMLPSLAIRLFAKAN
Sbjct: 181 YLIDALQGVNARVPYQSIIDPTATEDQIEEELYKLMTMPTRVFLVHMLPSLAIRLFAKAN 240

Query: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300
           +IGMMREGYVWILTDATTNLL SM SSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV
Sbjct: 241 QIGMMREGYVWILTDATTNLLDSMPSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300

Query: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360
           MENSV NDP+LDIFGLWAYDAARALAIA+EKTG KNFTFE+PNR +NLTDL+TLGVSRNG
Sbjct: 301 MENSVPNDPYLDIFGLWAYDAARALAIAVEKTGAKNFTFENPNRGQNLTDLETLGVSRNG 360

Query: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420
           EKI EALSKTKFMGLTGNYEIVNGQLQSA FEIVNVNSNGGNRVGFWNPEKGLLS++  M
Sbjct: 361 EKIVEALSKTKFMGLTGNYEIVNGQLQSAEFEIVNVNSNGGNRVGFWNPEKGLLSNN--M 420

Query: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480
           TVIWPGNT AVPKGW+F T GKRLRIGVPVK+GY EF++V G +VEGYC+DVFDA I  L
Sbjct: 421 TVIWPGNTAAVPKGWEFPTAGKRLRIGVPVKEGYSEFVRVNGKKVEGYCRDVFDAVIEAL 480

Query: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540
           PY +PFDYIPFAL NGSRAGSYD+LIMQV  GVYDGA+GD+TIVANRSKYVDFTLPFT+S
Sbjct: 481 PYALPFDYIPFALPNGSRAGSYDDLIMQVNTGVYDGAIGDITIVANRSKYVDFTLPFTDS 540

Query: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600
           GVSM+VPT+ANS NRAWLFLKPLT+ LWITSLCFFVF+GFVVWILEHRINP FRGPP+H 
Sbjct: 541 GVSMVVPTKANSMNRAWLFLKPLTVGLWITSLCFFVFMGFVVWILEHRINPAFRGPPSHH 600

Query: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660
           I TSLWYSFCTMVFAQRETLISNLARFVVVIWFFV+FVLTQSYTASLTSLLTVQ+LQP+I
Sbjct: 601 IGTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVMFVLTQSYTASLTSLLTVQQLQPTI 660

Query: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720
            NINELLTKQSWVGYL+GSFVWGLL SVGIKNLKSY+ PEELDKLLKLGGSNGGIDAAFD
Sbjct: 661 ININELLTKQSWVGYLDGSFVWGLLTSVGIKNLKSYRSPEELDKLLKLGGSNGGIDAAFD 720

Query: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780
           ETPY+KLFLSKFPNKYITA+PTYKTDGFGFAFSI SPLVADISRAVLNV ES KMNQLQM
Sbjct: 721 ETPYIKLFLSKFPNKYITANPTYKTDGFGFAFSIDSPLVADISRAVLNVRESEKMNQLQM 780

Query: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840
           KWFG+  NSWSS+ KVTSSRL+L +FWGLFLI G A+IISLFIYF IFLRKE HTL +T 
Sbjct: 781 KWFGED-NSWSSV-KVTSSRLSLSNFWGLFLIVGIAAIISLFIYFFIFLRKELHTLRNTT 840

Query: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900
            E SNSSIRSKIRALL+IYD RDLTS+TFRKSNPP+VVDNKIQANHG+ VG S +SNYLL
Sbjct: 841 TEASNSSIRSKIRALLRIYDNRDLTSHTFRKSNPPQVVDNKIQANHGDSVGASSSSNYLL 900

Query: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIV 939
           S SNDSIHDTNH F +S DL+PSNQ VE V+HTT EIV
Sbjct: 901 SSSNDSIHDTNHGFSKSGDLSPSNQDVETVIHTTNEIV 934

BLAST of CmaCh12G006580 vs. NCBI nr
Match: XP_023538261.1 (glutamate receptor 2.1-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1619.8 bits (4193), Expect = 0.0e+00
Identity = 814/938 (86.78%), Postives = 864/938 (92.11%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQNVSVGVVLDMESWVGKVGLSCIDMSLSEFYEA 60
           MKPN GS RALWFIA CLLLVATAGEAQNVSVGVVLDMESWVGK+GLSCIDMSLSEFYEA
Sbjct: 1   MKPNHGSHRALWFIAACLLLVATAGEAQNVSVGVVLDMESWVGKMGLSCIDMSLSEFYEA 60

Query: 61  NPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVP 120
           NPHY TRVVLHRRDSAGDVVGAAAAAL LI N+KVEAILGPTTSMQTNFVIKLAH +HVP
Sbjct: 61  NPHYKTRVVLHRRDSAGDVVGAAAAALHLINNNKVEAILGPTTSMQTNFVIKLAHKAHVP 120

Query: 121 ILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILP 180
           ILTFTAS+P+LASHRSPYFFRLTHTDSAQVAAIS L+K YNWR+VVLIYQDDEFGDG+ P
Sbjct: 121 ILTFTASTPALASHRSPYFFRLTHTDSAQVAAISALLKAYNWREVVLIYQDDEFGDGMSP 180

Query: 181 YLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKAN 240
           YLI ALQ VNARV Y+S+IDPT TED I EELYKLMTMPTRV +VHMLPSLAIRLFAKAN
Sbjct: 181 YLIHALQGVNARVPYQSIIDPTATEDHIGEELYKLMTMPTRVFVVHMLPSLAIRLFAKAN 240

Query: 241 EIGMMREGYVWILTDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFV 300
           EIGMMREGYVWILTDAT NLL SM+SSVLKSMEGALGVRTYVPKSMEL+RFKIKWKR+F+
Sbjct: 241 EIGMMREGYVWILTDATANLLDSMTSSVLKSMEGALGVRTYVPKSMELERFKIKWKRKFI 300

Query: 301 MENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNG 360
           MENSV NDP+LDIFGLWAYDAARALA+A+EKTG KNFTFE+PN SENLTDLQTLGVSRNG
Sbjct: 301 MENSVPNDPYLDIFGLWAYDAARALAMAVEKTGAKNFTFENPNGSENLTDLQTLGVSRNG 360

Query: 361 EKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETM 420
           EKI EALSKTKFMGLTGNYEI+NGQLQS  FEIVNVNSNGGNRVGFWN EKGLLS     
Sbjct: 361 EKIVEALSKTKFMGLTGNYEILNGQLQSTEFEIVNVNSNGGNRVGFWNTEKGLLSKD--- 420

Query: 421 TVIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVEGYCKDVFDAAIATL 480
           TVIWPGNT AVPKGW+F T GKRLRIGVPVKKGY EFMK+KG EVEGYCKDVFDAAI  L
Sbjct: 421 TVIWPGNTVAVPKGWEFPTGGKRLRIGVPVKKGYSEFMKLKGKEVEGYCKDVFDAAIEML 480

Query: 481 PYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTES 540
            Y V  DYIPFA+ +GS AGSYDELIMQVYKGVYDGAVGD+TIVANRSKYVDFTLPFT+S
Sbjct: 481 SYVVSVDYIPFAVSDGSSAGSYDELIMQVYKGVYDGAVGDITIVANRSKYVDFTLPFTDS 540

Query: 541 GVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQ 600
           GVSM+VPT+ANS NRAWLFLKPLT+ LWITSLCF VF+GFVVW LEHRINP FRGPP+HQ
Sbjct: 541 GVSMVVPTKANSMNRAWLFLKPLTVGLWITSLCFLVFMGFVVWFLEHRINPAFRGPPSHQ 600

Query: 601 ISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSI 660
           I TSLWYSFCTMVFAQRETLISNLAR VVVIWFFV+FVLTQSYTASLTSLLTVQ+LQP+I
Sbjct: 601 IGTSLWYSFCTMVFAQRETLISNLARLVVVIWFFVMFVLTQSYTASLTSLLTVQQLQPTI 660

Query: 661 TNINELLTKQSWVGYLEGSFVWGLLNSVGIKNLKSYKCPEELDKLLKLGGSNGGIDAAFD 720
           TNINELLTKQSWVGYL+GSFVWGLL SVGIKNLKSY+ PEELDKLLKLGGSNGGIDAAFD
Sbjct: 661 TNINELLTKQSWVGYLDGSFVWGLLTSVGIKNLKSYRSPEELDKLLKLGGSNGGIDAAFD 720

Query: 721 ETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQM 780
           ETPY+KLFLSKFPNKYITADPTYKTDGFGFAF IGS LVADISRAVLNV ES KMNQLQM
Sbjct: 721 ETPYIKLFLSKFPNKYITADPTYKTDGFGFAFPIGSLLVADISRAVLNVRESEKMNQLQM 780

Query: 781 KWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHTA 840
           KWFG   NSWSS+ KVTSSRLN+ +FWGLFLI GTA+I+SLFIYF IFLRKE HTL HT 
Sbjct: 781 KWFGGD-NSWSSV-KVTSSRLNVSNFWGLFLIVGTAAIVSLFIYFFIFLRKELHTLRHTT 840

Query: 841 NEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPVGTSPNSNYLL 900
            EGSNSSIRSKIRALL+IYDKRDLTS+TFRKSNPP+VVDNKIQANHG+ VG S +SNYLL
Sbjct: 841 TEGSNSSIRSKIRALLRIYDKRDLTSHTFRKSNPPQVVDNKIQANHGDSVGASSSSNYLL 900

Query: 901 SPSNDSIHDTNHEFFRSEDLNPSNQAVEMVVHTTMEIV 939
           SPSNDSIHDTNHEF +S DL+PSNQAVEMV+HTT EIV
Sbjct: 901 SPSNDSIHDTNHEFSKSGDLSPSNQAVEMVIHTTEEIV 933

BLAST of CmaCh12G006580 vs. TAIR 10
Match: AT2G29120.1 (glutamate receptor 2.7 )

HSP 1 Score: 816.6 bits (2108), Expect = 2.0e-236
Identity = 435/900 (48.33%), Postives = 592/900 (65.78%), Query Frame = 0

Query: 13  FIAGCLLLVATAGEAQ--NVSVGVVLDMESWVGKVGLSCIDMSLSEFYEANPHYNTRVVL 72
           F+ G +L+    G+ Q   + VGVVLD+ +   K+ L+ I++SLS+FY+ +  Y TR+ +
Sbjct: 20  FVCGFVLMEGCLGQNQTTEIKVGVVLDLHTSFSKLCLTSINISLSDFYKYHSDYTTRLAI 79

Query: 73  HRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVPILTFTASSPS 132
           H RDS  DVV A++AALDLIKN +V AI+GP TSMQ  F+I+LA  S VP +TF+A+ P 
Sbjct: 80  HIRDSMEDVVQASSAALDLIKNEQVSAIIGPRTSMQAEFMIRLADKSQVPTITFSATCPL 139

Query: 133 LASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILPYLIDALQAVN 192
           L S  SPYF R T  DS+QV AI+ +VK + WR VV IY D+EFG+GILP L DALQ V 
Sbjct: 140 LTSINSPYFVRATLDDSSQVKAIAAIVKSFGWRNVVAIYVDNEFGEGILPLLTDALQDVQ 199

Query: 193 ARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKANEIGMMREGYV 252
           A V+ R +I     +DQI +ELYKLMTM TRV +VHM P+L  R F KA EIGMM EGYV
Sbjct: 200 AFVVNRCLIPQEANDDQILKELYKLMTMQTRVFVVHMPPTLGFRFFQKAREIGMMEEGYV 259

Query: 253 WILTDATTNLLGSMS-SSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFVMENSVLNDP 312
           W+LTD   NLL S    S L++M+G LGVR+++PKS +L  F+++W++ F  +    ND 
Sbjct: 260 WLLTDGVMNLLKSNERGSSLENMQGVLGVRSHIPKSKKLKNFRLRWEKMFPKKG---NDE 319

Query: 313 HLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNRS-ENLTDLQTLGVSRNGEKIGEALS 372
            ++IF L AYD+  ALA+A+EKT +K+  ++ P  S  N T+L TLGVSR G  + +ALS
Sbjct: 320 EMNIFALRAYDSITALAMAVEKTNIKSLRYDHPIASGNNKTNLGTLGVSRYGPSLLKALS 379

Query: 373 KTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLLSSSETMT------- 432
             +F GL G +E++NGQL+S+ F+++N+  +    +G W P  G++++    T       
Sbjct: 380 NVRFNGLAGEFELINGQLESSVFDVINIIGSEERIIGLWRPSNGIVNAKSKNTTSVLGER 439

Query: 433 ---VIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEVE------GYCKDV 492
              VIWPG +  VPKGW   T GK LR+G+PVKKG++EF+  K   +       GYC ++
Sbjct: 440 LGPVIWPGKSKDVPKGWQIPTNGKMLRVGIPVKKGFLEFVDAKIDPISNAMTPTGYCIEI 499

Query: 493 FDAAIATLPYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVD 552
           F+A +  LPY V   YI F     S   +YDE++ QVY G YD  VGD+TIVANRS YVD
Sbjct: 500 FEAVLKKLPYSVIPKYIAFL----SPDENYDEMVYQVYTGAYDAVVGDVTIVANRSLYVD 559

Query: 553 FTLPFTESGVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPD 612
           FTLP+TESGVSM+VP + N     W+FL+P +LDLW+T+ CFFVFIGF+VWILEHR+N D
Sbjct: 560 FTLPYTESGVSMMVPLKDN--KNTWVFLRPWSLDLWVTTACFFVFIGFIVWILEHRVNTD 619

Query: 613 FRGPPTHQISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLT 672
           FRGPP HQI TS W++F TM FA RE ++SNLARFVV++W FVV VL QSYTA+LTS  T
Sbjct: 620 FRGPPHHQIGTSFWFAFSTMNFAHREKVVSNLARFVVLVWCFVVLVLIQSYTANLTSFFT 679

Query: 673 VQELQPSITNINELLTKQSWVGYLEGSFVWGLLNSVGI--KNLKSYKCPEELDKLLKLGG 732
           V+ LQP++TN  +L+     +GY  G+FV  LL S G     LK +    E D+L     
Sbjct: 680 VKLLQPTVTNWKDLIKFNKNIGYQRGTFVRELLKSQGFDESQLKPFGSAVECDELF---- 739

Query: 733 SNGGIDAAFDETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVT 792
           SNG I A+FDE  Y+K+ LS+  +KY   +P++KT GFGF F   SPL  D+SRA+LNVT
Sbjct: 740 SNGTITASFDEVAYIKVILSQNSSKYTMVEPSFKTAGFGFVFPKKSPLTDDVSRAILNVT 799

Query: 793 ESRKMNQLQMKWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLR 852
           +  +M  ++ KWF    N       ++S+ L+L SFWGLFLI G AS ++L I+   FL 
Sbjct: 800 QGEEMQHIENKWFKKPNNCPDLNTSLSSNHLSLSSFWGLFLIAGIASFLALLIFVANFLY 859

Query: 853 KEQHTLHHTANEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPEVVDNKIQANHGNPV 891
           + +HTL     + S +S R K++ L++ +D++D+ S+ F K N    V + I     +P+
Sbjct: 860 EHKHTLF----DDSENSFRGKLKFLVRNFDEKDIKSHMF-KENAVHNVSSPITQGSSSPL 901

BLAST of CmaCh12G006580 vs. TAIR 10
Match: AT2G29100.1 (glutamate receptor 2.9 )

HSP 1 Score: 811.6 bits (2095), Expect = 6.6e-235
Identity = 427/878 (48.63%), Postives = 595/878 (67.77%), Query Frame = 0

Query: 12  WFIAGCLLLVATAGEAQ--NVSVGVVLDMESWVGKVGLSCIDMSLSEFYEANPHYNTRVV 71
           +F+ G LL+    G+ Q   + VGVVLD+ +   K+ L+ I M++S+FY  +P+Y TR+ 
Sbjct: 10  YFVCGFLLMGVGLGQNQTSEIKVGVVLDLNTTFSKICLTSIKMAVSDFYADHPNYLTRLT 69

Query: 72  LHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVPILTFTASSP 131
           LH RDS  D V A+AAALDLIK  +V AI+GP  SMQ +F+IKLA+ + VP +TF+A+SP
Sbjct: 70  LHVRDSMEDTVQASAAALDLIKTEQVSAIIGPINSMQADFMIKLANKTQVPTITFSATSP 129

Query: 132 SLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILPYLIDALQAV 191
            L S +SPYF R T  DS+QV AI+ + KF+ WR+VV IY D+EFG+G +P+L DALQ V
Sbjct: 130 LLTSIKSPYFVRATIDDSSQVRAIASIFKFFRWRRVVAIYVDNEFGEGFMPFLFDALQDV 189

Query: 192 NARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKANEIGMMREGY 251
             +   RSVI P   +D+I++EL KLM    RV +VHM  SLA+R+F  A +IGMM EGY
Sbjct: 190 EVK---RSVIPPEAIDDEIQKELRKLMERQARVFVVHMESSLALRVFQIARDIGMMEEGY 249

Query: 252 VWILTDATTNLLGSMSSS-VLKSMEGALGVRTYVPKSMELDRFKIKWKREFVMENSVLND 311
           VW++T+  T+++  +++   L ++EG LGVR++VPKS EL  F+++WKR F  EN  + D
Sbjct: 250 VWLMTNGMTHMMRHINNGRSLNTIEGVLGVRSHVPKSKELGDFRLRWKRTFEKENPSMRD 309

Query: 312 PHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPNR-SENLTDLQTLGVSRNGEKIGEAL 371
             L++F LWAYD+  ALA A+EK   K+  +++ +  S+N TDL  +GVS  G  + +A 
Sbjct: 310 -DLNVFALWAYDSITALAKAVEKANTKSLWYDNGSTLSKNRTDLGNVGVSLYGPSLQKAF 369

Query: 372 SKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLL----SSSETM-TV 431
           S+ +F GL G +++++GQLQS  FEI+N   N    +GFW P  GL+    S+ +T+  V
Sbjct: 370 SEVRFNGLAGEFKLIDGQLQSPKFEIINFVGNEERIIGFWTPRDGLMDATSSNKKTLGPV 429

Query: 432 IWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKVKGTEV------EGYCKDVFDAA 491
           IWPG +  VPKGW+    GK+LR+GVP+KKG+ +F+KV    +       GY  ++F+AA
Sbjct: 430 IWPGKSKIVPKGWEI--PGKKLRVGVPMKKGFFDFVKVTINPITNKKTPTGYAIEIFEAA 489

Query: 492 IATLPYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLP 551
           +  LPY V  +Y+ F   N     +Y+ L+ QVY   +D  VGD+TI ANRS Y DFTLP
Sbjct: 490 LKELPYLVIPEYVSFESPN-----NYNNLVYQVYDKTWDAVVGDITITANRSLYADFTLP 549

Query: 552 FTESGVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGP 611
           FTESGVSM+VP + N     W+FL+P +L+LW+T+ CFFVFIGFVVW+ EHR+N DFRGP
Sbjct: 550 FTESGVSMMVPVRDNENKDTWVFLEPWSLELWVTTGCFFVFIGFVVWLFEHRVNTDFRGP 609

Query: 612 PTHQISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQEL 671
           P +QI TSLW+SF TMVFA RE ++SNLARFVVV+W FVV VLTQSYTASLTS LTVQ L
Sbjct: 610 PQYQIGTSLWFSFSTMVFAHRENVVSNLARFVVVVWCFVVLVLTQSYTASLTSFLTVQSL 669

Query: 672 QPSITNINELLTKQSWVGYLEGSFVWGLLNSVGI--KNLKSYKCPEELDKLLKLGGSNGG 731
           QP++TN+N+L+  +  VGY  G+FV  +L  +G     LK +   ++ D LL  G S  G
Sbjct: 670 QPTVTNVNDLIKNRDCVGYQGGAFVKDILLGLGFHEDQLKPFDSAKDADDLLSKGKSK-G 729

Query: 732 IDAAFDETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRK 791
           I AAFDE  YLK  LS+  +KY+  +PT+KT GFGFAF   SPL  + SRA+LN+T++  
Sbjct: 730 IAAAFDEVAYLKAILSQSCSKYVMVEPTFKTGGFGFAFPKNSPLTGEFSRAILNLTQNNV 789

Query: 792 MNQLQMKWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQH 851
             Q++ +WF  + +    +  ++S+RLNL SF GLFLI GTA   SL ++  +FL + +H
Sbjct: 790 TQQIEDRWFPKKNDCPDPMTALSSNRLNLSSFLGLFLIAGTAISFSLLVFVALFLYEHRH 849

Query: 852 TLHHTANEGSNSSIRSKIRALLKIYDKRDLTSYTFRKS 873
           TL     + S  S+  K++ L KI+D++D+ S+TF+ S
Sbjct: 850 TL----GDDSEDSLWRKLKFLFKIFDEKDMNSHTFKNS 871

BLAST of CmaCh12G006580 vs. TAIR 10
Match: AT2G29110.1 (glutamate receptor 2.8 )

HSP 1 Score: 810.1 bits (2091), Expect = 1.9e-234
Identity = 442/936 (47.22%), Postives = 596/936 (63.68%), Query Frame = 0

Query: 1   MKPNQGSRRALWFIAGCLLLVATAGEAQN----VSVGVVLDMESWVGKVGLSCIDMSLSE 60
           M P + +   L +   CL L+   G  QN    + VGVVLD+ +   K+ L+ I+++LS+
Sbjct: 1   MNPKKNNNTFLSYFV-CLFLLLEVGLGQNQISEIKVGVVLDLNTTFSKICLTSINLALSD 60

Query: 61  FYEANPHYNTRVVLHRRDSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHN 120
           FY+ +P+Y TR+ LH RDS  D V A+AAALDLI+N +V AI+GP  SMQ  F+IKLA+ 
Sbjct: 61  FYKDHPNYRTRLALHVRDSMKDTVQASAAALDLIQNEQVSAIIGPIDSMQAKFMIKLANK 120

Query: 121 SHVPILTFTASSPSLASHRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGD 180
           + VP ++F+A+SP L S +S YF R T  DS QV AI+ + + + WR VV IY D+E G+
Sbjct: 121 TQVPTISFSATSPLLTSIKSDYFVRGTIDDSYQVKAIAAIFESFGWRSVVAIYVDNELGE 180

Query: 181 GILPYLIDALQAVNARVLYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLF 240
           GI+PYL DALQ V    + RSVI     +DQI +ELYKLMT  TRV +VHM   LA R+F
Sbjct: 181 GIMPYLFDALQDVQ---VDRSVIPSEANDDQILKELYKLMTRQTRVFVVHMASRLASRIF 240

Query: 241 AKANEIGMMREGYVWILTDATTNLLGSM-SSSVLKSMEGALGVRTYVPKSMELDRFKIKW 300
            KA EIGMM EGYVW++T+  T+++  +     L +++G LGVR++VPKS  L+ F+++W
Sbjct: 241 EKATEIGMMEEGYVWLMTNGMTHMMRHIHHGRSLNTIDGVLGVRSHVPKSKGLEDFRLRW 300

Query: 301 KREFVMENSVLNDPHLDIFGLWAYDAARALAIAIEKTGVKNFTFESPN-RSENLTDLQTL 360
           KR F  EN  L D  L IFGLWAYD+  ALA+A+EKT + +F + + +  S N+TDL TL
Sbjct: 301 KRNFKKENPWLRD-DLSIFGLWAYDSTTALAMAVEKTNISSFPYNNASGSSNNMTDLGTL 360

Query: 361 GVSRNGEKIGEALSKTKFMGLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLL 420
            VSR G  + EALS+ +F GL G + +++ QL+S  FEI+N   N    VGFW P  GL+
Sbjct: 361 HVSRYGPSLLEALSEIRFNGLAGRFNLIDRQLESPKFEIINFVGNEERIVGFWTPSNGLV 420

Query: 421 SSSETMT----------VIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMKV---- 480
           + +   T          +IWPG +T VPKGW+  T GK++++GVPVKKG+  F++V    
Sbjct: 421 NVNSNKTTSFTGERFGPLIWPGKSTIVPKGWEIPTNGKKIKVGVPVKKGFFNFVEVITDP 480

Query: 481 --KGTEVEGYCKDVFDAAIATLPYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAV 540
               T  +GY  D+F+AA+  LPY V   Y  F     S    YD+L+ +V  G  D  V
Sbjct: 481 ITNITTPKGYAIDIFEAALKKLPYSVIPQYYRFE----SPDDDYDDLVYKVDNGTLDAVV 540

Query: 541 GDLTIVANRSKYVDFTLPFTESGVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFI 600
           GD+TI A RS Y DFTLP+TESGVSM+VP + N     W+FLKP  LDLW+T+ CFFV I
Sbjct: 541 GDVTITAYRSLYADFTLPYTESGVSMMVPVRDNENKNTWVFLKPWGLDLWVTTACFFVLI 600

Query: 601 GFVVWILEHRINPDFRGPPTHQISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFV 660
           GFVVW+ EHR+N DFRGPP HQI TS W+SF TMVFA RE ++SNLARFVVV+W FVV V
Sbjct: 601 GFVVWLFEHRVNTDFRGPPHHQIGTSFWFSFSTMVFAHREKVVSNLARFVVVVWCFVVLV 660

Query: 661 LTQSYTASLTSLLTVQELQPSITNINELLTKQSWVGYLEGSFVWGLLNSVG--IKNLKSY 720
           LTQSYTA+LTS LTVQ  QP+  N+ +L+    +VGY  G+FV   L   G  +  LK +
Sbjct: 661 LTQSYTANLTSFLTVQRFQPAAINVKDLIKNGDYVGYQHGAFVKDFLIKEGFNVSKLKPF 720

Query: 721 KCPEELDKLLKLGGSNGGIDAAFDETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGS 780
              EE   LL    SNG I AAFDE  YL+  LS++ +KY   +PT+KT GFGFAF   S
Sbjct: 721 GSSEECHALL----SNGSISAAFDEVAYLRAILSQYCSKYAIVEPTFKTAGFGFAFPRNS 780

Query: 781 PLVADISRAVLNVTESRKMNQLQMKWFGDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTA 840
           PL  D+S+A+LNVT+  +M  ++ KWF  Q +       ++S+RL+L SFWGLFLI G A
Sbjct: 781 PLTGDVSKAILNVTQGDEMQHIENKWFMKQNDCPDPKTALSSNRLSLRSFWGLFLIAGIA 840

Query: 841 SIISLFIYFIIFLRKEQHTLHHTANEGSNSSIRSKIRALLKIYDKRDLTSYTFRKSNPPE 900
           S ++L I+  +FL + +HTL     + S  SI  K+ +L + +D++D+ S+TF+ S    
Sbjct: 841 SFLALLIFVFLFLYENRHTL----CDDSEDSIWRKLTSLFRNFDEKDIKSHTFKSSAVHH 900

Query: 901 VVDNKIQANHGNPVGTSPNSNYLLSPSNDSIHDTNH 913
           V              +SP + Y+ SPS   I    H
Sbjct: 901 V--------------SSPMTQYIPSPSTLQIAPRPH 905

BLAST of CmaCh12G006580 vs. TAIR 10
Match: AT5G27100.1 (glutamate receptor 2.1 )

HSP 1 Score: 782.3 bits (2019), Expect = 4.3e-226
Identity = 407/848 (48.00%), Postives = 554/848 (65.33%), Query Frame = 0

Query: 18  LLLVATAGEAQ----NVSVGVVLDMESWVGKVGLSCIDMSLSEFYEANPHYNTRVVLHRR 77
           ++ +   GEAQ    NV+VG+V D+ +    + L CI+MSLS+FY ++P   TR+V    
Sbjct: 16  IVFLMQVGEAQNRITNVNVGIVNDIGTAYSNMTLLCINMSLSDFYSSHPETQTRLVTTVV 75

Query: 78  DSAGDVVGAAAAALDLIKNHKVEAILGPTTSMQTNFVIKLAHNSHVPILTFTASSPSLAS 137
           DS  DVV AAAAALDLI N +V+AILGP TSMQ  F+I++   S VPI+T++A+SPSLAS
Sbjct: 76  DSKNDVVTAAAAALDLITNKEVKAILGPWTSMQAQFMIEMGQKSQVPIVTYSATSPSLAS 135

Query: 138 HRSPYFFRLTHTDSAQVAAISDLVKFYNWRQVVLIYQDDEFGDGILPYLIDALQAVNARV 197
            RS YFFR T+ DS+QV AI +++K + WR+V  +Y DD FG+GI+P L D LQ +N R+
Sbjct: 136 IRSQYFFRATYDDSSQVHAIKEIIKLFGWREVAPVYVDDTFGEGIMPRLTDVLQEINVRI 195

Query: 198 LYRSVIDPTPTEDQIEEELYKLMTMPTRVLLVHMLPSLAIRLFAKANEIGMMREGYVWIL 257
            YR+VI P  T+D+I  EL ++MT+PTRV +VH++  LA R FAKA EIG+M++GYVWIL
Sbjct: 196 PYRTVISPNATDDEISVELLRMMTLPTRVFVVHLVELLASRFFAKATEIGLMKQGYVWIL 255

Query: 258 TDATTNLLGSMSSSVLKSMEGALGVRTYVPKSMELDRFKIKWKREFVMENSVLNDPHLDI 317
           T+  T++L  M+ + +++M+G LGV+TYVP+S EL+ F+ +W + F + +       L++
Sbjct: 256 TNTITDVLSIMNETEIETMQGVLGVKTYVPRSKELENFRSRWTKRFPISD-------LNV 315

Query: 318 FGLWAYDAARALAIAIEKTGVKNFTFESPNRSENLTDLQTLGVSRNGEKIGEALSKTKFM 377
           +GLWAYDA  ALA+AIE+ G  N TF   +   N+++LQ LGVS+ G K+ + LS+ +F 
Sbjct: 316 YGLWAYDATTALALAIEEAGTSNLTFVKMDAKRNVSELQGLGVSQYGPKLLQTLSRVRFQ 375

Query: 378 GLTGNYEIVNGQLQSAAFEIVNVNSNGGNRVGFWNPEKGLL-------SSSETMT----- 437
           GL G+++ +NG+LQ + FEIVNVN  GG  +GFW  E GL        +S  T +     
Sbjct: 376 GLAGDFQFINGELQPSVFEIVNVNGQGGRTIGFWMKEYGLFKNVDQKPASKTTFSSWQDR 435

Query: 438 ---VIWPGNTTAVPKGWDFQTTGKRLRIGVPVKKGYIEFMK------VKGTEVEGYCKDV 497
              +IWPG+TT+VPKGW+  T GKRL+IGVPV   + +F+K         T   G+  D 
Sbjct: 436 LRPIIWPGDTTSVPKGWEIPTNGKRLQIGVPVNNTFQQFVKATRDPITNSTIFSGFSIDY 495

Query: 498 FDAAIATLPYDVPFDYIPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVD 557
           F+A I  +PYD+ +D+IPF      + G YD L+ QVY G YD  V D TI +NRS YVD
Sbjct: 496 FEAVIQAIPYDISYDFIPF------QDGGYDALVYQVYLGKYDAVVADTTISSNRSMYVD 555

Query: 558 FTLPFTESGVSMIVPTQANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPD 617
           F+LP+T SGV ++VP + + +  + +FL PLTL LW+ SL  F  IG VVW+LEHR+NPD
Sbjct: 556 FSLPYTPSGVGLVVPVKDSVRRSSTIFLMPLTLALWLISLLSFFIIGLVVWVLEHRVNPD 615

Query: 618 FRGPPTHQISTSLWYSFCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLT 677
           F GP  +Q+ST  W+SF  MVFA RE ++S  AR VV+IW+F+V VLTQSYTASL SLLT
Sbjct: 616 FDGPGQYQLSTIFWFSFSIMVFAPRERVLSFWARVVVIIWYFLVLVLTQSYTASLASLLT 675

Query: 678 VQELQPSITNINELLTKQSWVGYLEGSFVWGLLNSVGIK--NLKSYKCPEELDKLLKLGG 737
            Q L P++TNIN LL K   VGY + SF+ G L   G    +L SY  PE  D LL  G 
Sbjct: 676 TQHLHPTVTNINSLLAKGESVGY-QSSFILGRLRDSGFSEASLVSYGSPEHCDALLSKGQ 735

Query: 738 SNGGIDAAFDETPYLKLFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVT 797
           + GG+ A   E PY+++FL ++ NKY      +K DG GF F IGSPLVADISRA+L V 
Sbjct: 736 AEGGVSAVLMEVPYVRIFLGQYCNKYKMVQTPFKVDGLGFVFPIGSPLVADISRAILKVE 795

Query: 798 ESRKMNQLQMKWF-------GDQGNSWSSIPKVTSSRLNLDSFWGLFLITGTASIISLFI 832
           ES K NQL+  WF        D   +    P V+  +L  DSFW LFL+      ++L  
Sbjct: 796 ESNKANQLENAWFKPIDESCPDPLTNPDPNPSVSFRQLGFDSFWVLFLVAAIVCTMALLK 849

BLAST of CmaCh12G006580 vs. TAIR 10
Match: AT2G24720.1 (glutamate receptor 2.2 )

HSP 1 Score: 761.9 bits (1966), Expect = 6.0e-220
Identity = 399/868 (45.97%), Postives = 561/868 (64.63%), Query Frame = 0

Query: 30  VSVGVVLDMESWVGKVGLSCIDMSLSEFYEANPHYNTRVVLHRRDSAGDVVGAAAAALDL 89
           V++GVV D+ +    V + CI+MSL++FY + P + TR+V++  DS  DVVGAA AA+DL
Sbjct: 32  VNIGVVSDVGTSYPDVAMLCINMSLADFYSSRPQFQTRLVVNVGDSKNDVVGAATAAIDL 91

Query: 90  IKNHKVEAILGPTTSMQTNFVIKLAHNSHVPILTFTASSPSLASHRSPYFFRLTHTDSAQ 149
           IKN +V+AILGP TSMQ +F+I++   S VP+++++A+SPSL S RSPYFFR T+ DS+Q
Sbjct: 92  IKNKQVKAILGPWTSMQAHFLIEIGQKSRVPVVSYSATSPSLTSLRSPYFFRATYEDSSQ 151

Query: 150 VAAISDLVKFYNWRQVVLIYQDDEFGDGILPYLIDALQAVNARVLYRSVIDPTPTEDQIE 209
           V AI  ++K + WR+VV +Y D+ FG+GI+P L D+LQ +N R+ YRSVI    T+  I 
Sbjct: 152 VHAIKAIIKLFGWREVVPVYIDNTFGEGIMPRLTDSLQDINVRIPYRSVIPLNATDQDIS 211

Query: 210 EELYKLMTMPTRVLLVHMLPSLAIRLFAKANEIGMMREGYVWILTDATTNLLGSMSSSVL 269
            EL K+M MPTRV +VHM  SLA  +F KA E+G+M+ GYVWILT+   + L S++ + +
Sbjct: 212 VELLKMMNMPTRVFIVHMSSSLASTVFIKAKELGLMKPGYVWILTNGVMDGLRSINETGI 271

Query: 270 KSMEGALGVRTYVPKSMELDRFKIKWKREFVMENSVLNDPHLDIFGLWAYDAARALAIAI 329
           ++MEG LG++TY+PKS +L+ F+ +WKR F           L+++GLWAYDA  ALA+AI
Sbjct: 272 EAMEGVLGIKTYIPKSKDLETFRSRWKRRF-------PQMELNVYGLWAYDATTALAMAI 331

Query: 330 EKTGVKNFTFESPNRSENLTDLQTLGVSRNGEKIGEALSKTKFMGLTGNYEIVNGQLQSA 389
           E  G+ N TF + +  +N+++L  LG+S+ G K+ + +S  +F GL G++  V+GQLQ +
Sbjct: 332 EDAGINNMTFSNVDTGKNVSELDGLGLSQFGPKLLQTVSTVQFKGLAGDFHFVSGQLQPS 391

Query: 390 AFEIVNVNSNGGNRVGFWNPEKGLLS-------SSETMT--------VIWPGNTTAVPKG 449
            FEIVN+   G   +GFW    GL+        S  T++        +IWPG   +VPKG
Sbjct: 392 VFEIVNMIGTGERSIGFWTEGNGLVKKLDQEPRSIGTLSTWPDHLKHIIWPGEAVSVPKG 451

Query: 450 WDFQTTGKRLRIGVPVKKGYIEFMKV------KGTEVEGYCKDVFDAAIATLPYDVPFDY 509
           W+  T GK+LRIGVP + G+ + +KV        T V+G+C D F+A I  +PYDV +++
Sbjct: 452 WEIPTNGKKLRIGVPKRIGFTDLVKVTRDPITNSTVVKGFCIDFFEAVIQAMPYDVSYEF 511

Query: 510 IPFALLNGSRAGSYDELIMQVYKGVYDGAVGDLTIVANRSKYVDFTLPFTESGVSMIVPT 569
            PF   NG  AG++++L+ QVY G +D  VGD TI+ANRS +VDFTLPF +SGV +IVP 
Sbjct: 512 FPFEKPNGEPAGNHNDLVHQVYLGQFDAVVGDTTILANRSSFVDFTLPFMKSGVGLIVPL 571

Query: 570 QANSKNRAWLFLKPLTLDLWITSLCFFVFIGFVVWILEHRINPDFRGPPTHQISTSLWYS 629
           +   K   + FLKPL+++LW+T+L FF  +G  VW LEHR+N DFRGP  +Q ST  W++
Sbjct: 572 KDEVKRDKFSFLKPLSIELWLTTLVFFFLVGISVWTLEHRVNSDFRGPANYQASTIFWFA 631

Query: 630 FCTMVFAQRETLISNLARFVVVIWFFVVFVLTQSYTASLTSLLTVQELQPSITNINELLT 689
           F TMVFA RE ++S  AR +VV W+FV+ VLTQSYTASL SLLT Q+L P+IT+++ LL 
Sbjct: 632 FSTMVFAPRERVLSFGARSLVVTWYFVLLVLTQSYTASLASLLTSQQLNPTITSMSSLLH 691

Query: 690 KQSWVGYLEGSFVWGLLNSVGI--KNLKSYKCPEELDKLLKLGGSNGGIDAAFDETPYLK 749
           +   VGY   SF+ G LN  G    +L  +   EE D+LLK G  NGG+ AAF  TPY++
Sbjct: 692 RGETVGYQRTSFILGKLNETGFPQSSLVPFDTAEECDELLKKGPKNGGVAAAFLGTPYVR 751

Query: 750 LFLSKFPNKYITADPTYKTDGFGFAFSIGSPLVADISRAVLNVTESRKMNQLQMKWFGDQ 809
           LFL ++ N Y   +  +  DGFGF F IGSPLVAD+SRA+L V ES K  +L+  WF  +
Sbjct: 752 LFLGQYCNTYKMVEEPFNVDGFGFVFPIGSPLVADVSRAILKVAESPKAVELEHAWFKKK 811

Query: 810 GNSW-------SSIPKVTSSRLNLDSFWGLFLITGTASIISLFIYFIIFLRKEQHTLHHT 868
             S         S P VT+ +L + SFW LFL+     +++L  +   FL K        
Sbjct: 812 EQSCPDPVTNPDSNPTVTAIQLGVGSFWFLFLVVFVVCVLALGKFTFCFLWK-------- 871