CmaCh12G006400 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G006400
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionzinc transporter 4, chloroplastic-like
LocationCma_Chr12: 3405585 .. 3407788 (-)
RNA-Seq ExpressionCmaCh12G006400
SyntenyCmaCh12G006400
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
AGAGAGAGAGAGATGAAGGGCAAAAAGGGAATGAAATAAAAATGCAAAAGGAGGGCGTTAAGAGTGTCGACACTATCGGCCTCATTGTTAAGCAAGAAGTCATAACTGTAGCTCATGCCTCTGCGTCTGCCCTCCATTTCATTCCATTATGTCGACACTCTTCTCTCTTCCCTTCTCCCCCATTTCTCACCTTCTTTCTCTCTTCTCCTCCTTCTCCGATCGCTCGCTGCCGCTTCTCTTTTATAAACTCTTCAAACAATCCCCCTTCCATCCTCATTCCATTTCCGGCGCCGTTTCTTTTCACCATTTTAATCTATTCTCATGCTGTTCTTCGAGGTTCCAATCCCACCCCATTCTTCCCTCTTTGGTTTCTCTGATTTCTCCTCGTTTATATGTTTTCTGTTCTTCAGGATTTGTGGCAGATGCTCTGGTTTGAAGGTTTCCAAGATCAAACTCGACTTCTCTCTGGTAATTTCTCCATTCTCGTTTGTTTTCGCCTTAATTCGTCTATTCCGCTTCCAATTGAACTGAGTCTTTCTGAATTTTGTGGATTCCTAATTTCTTGGAGTTTCTGTATTCTTGTTATTTATGGATTAATTTATTGATTCTTCGTTTCTAGGTTTTAGTTAGATTAGGTTTGTGTAATTGATTTCTAATCTGTGGAATTTTCGTTCTCCATTTGTAATCAAACAGAGTCCATTGTACGGACCGTATCCGAATCAATGGGCAATTCATCCTGTAGTGTATCTGAGTTAGAACTCTGTAGAGATGATTCGGCAGCGCTTAATTTCAAATTAATCGCCATTGCCTCGATTCTCACATCGGGAGTTTTCGGAATTGCCATTCCGTTATTCGGCAGACATCGCCGGTTTCTCAAAACGGATGGCAATCTCTTTGTAGCGACTAAGGCGTTTGCTGCCGGCGTGATTCTTGCGACGGCGTTTGTTCATATGCTTCCCGATGGATCGAAAGCTCTCTCCGATCCGTGCTTGCCGGAATTTCCCTGGTCGAAATTCCCTTTCTCTGGATTCTTCGCGATGATGGCGTCGCTTTTGACGCTCCTTGTCGATTTCGTCGGAACTCAGTATTACGAGAGGAAACAGGGGCAGATTCGATTGAAAGAAGAAACGAATCGGGTCGGGTCGGGTTTGGATCACGGGTTGGAGGCTGGGATTCTCCCGGCCGCCGATTCGAACGTGAAAGTGTTCGGCGAAGAGGATGGCGGCGGGATGCACATCGTCGGAATGCATGCACACGCCGCGCAACACCGCCATAGTCATCCGCAGGGGAAAGAGGCATGTGACGGGCACGTGACACTCCATGATCATGCCCATGGTCATTCTCATGGGCTCGACGGCGACGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGGTAATCCATTTATCTTCTCGATTAATGAAAATTCCCGAATATTGACGTCATTATTCTATGGGACAGATTTTGGAGCTTGGAATAGTGTCGCATTCAGTGATAATTGGGCTGTCTCTGGGAGTGTCCCACAGCCCATGTACTATAAGACCCTTAATTGCAGCTCTCTCGTTCCATCAATTCTTTGAAGGCTTTGCTCTTGGTGGCTGCATCTCCCAGGCTCAGTTCAACACCCTCTCAACCTCCCTCATGGCTGTGTTCTTCGCCGCCACCACGCCCGTTGGGATTGCCATCGGTGCGGCGATCTCCTCCACCTACAACCCGAACAGCACCGGAGCGTTGGTTGCCGAAGGCATTTTGGACTCTCTATCAGCTGGGATTCTGGTGTATATGGCTTTGGTTGATTTGATCGCTGCTGACTTTCTTAGCAAGAGAATGAGCTGCAATTTTCGACTCCAAATTGTTTCGTATTGTACACTCTTTCTCGGTGCGGGATTGATGTCGTTGCTCGCGCTATGGGCTTGATCATAAGTTCTATTTGTTTTTTCGTCAGGAAATTGTTTTTTTCAACATTGTTAGGAGGAAGAAATGGAAAGAGATAGATGATGTTAGGGAATTAATGGTTTTCGTAAGGGTATGTACACCCCGAAATCCCTTTTGGAATTCGACAAAAATGCATCAACTGACATAATGTCGTAACTAGTTGAGTTATCGACAAAAATGCATCAACTGACATAATGTCGTAACTAGTTGAGTTATCGACAAAAATGCATCAACTGGCATAATGTCGTAACTAGTTGAGTTATC

mRNA sequence

AGAGAGAGAGAGATGAAGGGCAAAAAGGGAATGAAATAAAAATGCAAAAGGAGGGCGTTAAGAGTGTCGACACTATCGGCCTCATTGTTAAGCAAGAAGTCATAACTGTAGCTCATGCCTCTGCGTCTGCCCTCCATTTCATTCCATTATGTCGACACTCTTCTCTCTTCCCTTCTCCCCCATTTCTCACCTTCTTTCTCTCTTCTCCTCCTTCTCCGATCGCTCGCTGCCGCTTCTCTTTTATAAACTCTTCAAACAATCCCCCTTCCATCCTCATTCCATTTCCGGCGCCGTTTCTTTTCACCATTTTAATCTATTCTCATGCTGTTCTTCGAGGATTTGTGGCAGATGCTCTGGTTTGAAGGTTTCCAAGATCAAACTCGACTTCTCTCTGAGTCCATTGTACGGACCGTATCCGAATCAATGGGCAATTCATCCTGTAGTGTATCTGAGTTAGAACTCTGTAGAGATGATTCGGCAGCGCTTAATTTCAAATTAATCGCCATTGCCTCGATTCTCACATCGGGAGTTTTCGGAATTGCCATTCCGTTATTCGGCAGACATCGCCGGTTTCTCAAAACGGATGGCAATCTCTTTGTAGCGACTAAGGCGTTTGCTGCCGGCGTGATTCTTGCGACGGCGTTTGTTCATATGCTTCCCGATGGATCGAAAGCTCTCTCCGATCCGTGCTTGCCGGAATTTCCCTGGTCGAAATTCCCTTTCTCTGGATTCTTCGCGATGATGGCGTCGCTTTTGACGCTCCTTGTCGATTTCGTCGGAACTCAGTATTACGAGAGGAAACAGGGGCAGATTCGATTGAAAGAAGAAACGAATCGGGTCGGGTCGGGTTTGGATCACGGGTTGGAGGCTGGGATTCTCCCGGCCGCCGATTCGAACGTGAAAGTGTTCGGCGAAGAGGATGGCGGCGGGATGCACATCGTCGGAATGCATGCACACGCCGCGCAACACCGCCATAGTCATCCGCAGGGGAAAGAGGCATGTGACGGGCACGTGACACTCCATGATCATGCCCATGGTCATTCTCATGGGCTCGACGGCGACGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGATTTTGGAGCTTGGAATAGTGTCGCATTCAGTGATAATTGGGCTGTCTCTGGGAGTGTCCCACAGCCCATGTACTATAAGACCCTTAATTGCAGCTCTCTCGTTCCATCAATTCTTTGAAGGCTTTGCTCTTGGTGGCTGCATCTCCCAGGCTCAGTTCAACACCCTCTCAACCTCCCTCATGGCTGTGTTCTTCGCCGCCACCACGCCCGTTGGGATTGCCATCGGTGCGGCGATCTCCTCCACCTACAACCCGAACAGCACCGGAGCGTTGGTTGCCGAAGGCATTTTGGACTCTCTATCAGCTGGGATTCTGGTGTATATGGCTTTGGTTGATTTGATCGCTGCTGACTTTCTTAGCAAGAGAATGAGCTGCAATTTTCGACTCCAAATTGTTTCGTATTGTACACTCTTTCTCGGTGCGGGATTGATGTCGTTGCTCGCGCTATGGGCTTGATCATAAGTTCTATTTGTTTTTTCGTCAGGAAATTGTTTTTTTCAACATTGTTAGGAGGAAGAAATGGAAAGAGATAGATGATGTTAGGGAATTAATGGTTTTCGTAAGGGTATGTACACCCCGAAATCCCTTTTGGAATTCGACAAAAATGCATCAACTGACATAATGTCGTAACTAGTTGAGTTATCGACAAAAATGCATCAACTGACATAATGTCGTAACTAGTTGAGTTATCGACAAAAATGCATCAACTGGCATAATGTCGTAACTAGTTGAGTTATC

Coding sequence (CDS)

ATGCTGTTCTTCGAGGATTTGTGGCAGATGCTCTGGTTTGAAGGTTTCCAAGATCAAACTCGACTTCTCTCTGAGTCCATTGTACGGACCGTATCCGAATCAATGGGCAATTCATCCTGTAGTGTATCTGAGTTAGAACTCTGTAGAGATGATTCGGCAGCGCTTAATTTCAAATTAATCGCCATTGCCTCGATTCTCACATCGGGAGTTTTCGGAATTGCCATTCCGTTATTCGGCAGACATCGCCGGTTTCTCAAAACGGATGGCAATCTCTTTGTAGCGACTAAGGCGTTTGCTGCCGGCGTGATTCTTGCGACGGCGTTTGTTCATATGCTTCCCGATGGATCGAAAGCTCTCTCCGATCCGTGCTTGCCGGAATTTCCCTGGTCGAAATTCCCTTTCTCTGGATTCTTCGCGATGATGGCGTCGCTTTTGACGCTCCTTGTCGATTTCGTCGGAACTCAGTATTACGAGAGGAAACAGGGGCAGATTCGATTGAAAGAAGAAACGAATCGGGTCGGGTCGGGTTTGGATCACGGGTTGGAGGCTGGGATTCTCCCGGCCGCCGATTCGAACGTGAAAGTGTTCGGCGAAGAGGATGGCGGCGGGATGCACATCGTCGGAATGCATGCACACGCCGCGCAACACCGCCATAGTCATCCGCAGGGGAAAGAGGCATGTGACGGGCACGTGACACTCCATGATCATGCCCATGGTCATTCTCATGGGCTCGACGGCGACGATGACGAAAGCGGTGTACGACATGTCGTTGTTTCCCAGATTTTGGAGCTTGGAATAGTGTCGCATTCAGTGATAATTGGGCTGTCTCTGGGAGTGTCCCACAGCCCATGTACTATAAGACCCTTAATTGCAGCTCTCTCGTTCCATCAATTCTTTGAAGGCTTTGCTCTTGGTGGCTGCATCTCCCAGGCTCAGTTCAACACCCTCTCAACCTCCCTCATGGCTGTGTTCTTCGCCGCCACCACGCCCGTTGGGATTGCCATCGGTGCGGCGATCTCCTCCACCTACAACCCGAACAGCACCGGAGCGTTGGTTGCCGAAGGCATTTTGGACTCTCTATCAGCTGGGATTCTGGTGTATATGGCTTTGGTTGATTTGATCGCTGCTGACTTTCTTAGCAAGAGAATGAGCTGCAATTTTCGACTCCAAATTGTTTCGTATTGTACACTCTTTCTCGGTGCGGGATTGATGTCGTTGCTCGCGCTATGGGCTTGA

Protein sequence

MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLIAIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA
Homology
BLAST of CmaCh12G006400 vs. ExPASy Swiss-Prot
Match: Q8LE59 (Fe(2+) transport protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IRT3 PE=2 SV=3)

HSP 1 Score: 506.1 bits (1302), Expect = 3.6e-142
Identity = 273/426 (64.08%), Postives = 324/426 (76.06%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLS--ESIVRTVSESMGNSS----CSVSELELCRDDSAA 60
           M F + LW+++    F  +T+ LS  ESI++ V E+M  +S    C+ SE +LCRDDSAA
Sbjct: 1   MFFVDVLWKLVPLYLFGSETKSLSATESILQIVPEAMAATSSNVLCNASESDLCRDDSAA 60

Query: 61  LNFKLIAIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPD 120
              K +AIASIL +G  G+ IPL GR+RRFL+TDGNLFV  KAFAAGVILAT FVHML  
Sbjct: 61  FLLKFVAIASILLAGAAGVTIPLIGRNRRFLQTDGNLFVTAKAFAAGVILATGFVHMLAG 120

Query: 121 GSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVG 180
           G++AL +PCLP+FPWSKFPF GFFAM+A+L+TL VDF+GTQYYERKQ +    E     G
Sbjct: 121 GTEALKNPCLPDFPWSKFPFPGFFAMIAALITLFVDFMGTQYYERKQ-EREASESVEPFG 180

Query: 181 SGLDHGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTL- 240
                G+   ++    ++ KVFGEED GG+HIVG+HAHAA HRHSHP G ++C+GH  + 
Sbjct: 181 REQSPGIVVPMIGEGTNDGKVFGEEDSGGIHIVGIHAHAAHHRHSHPPGHDSCEGHSKID 240

Query: 241 --HDHAHGHSHG------LDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCT 300
             H HAHGH HG        G D  +G RH+VVSQ+LELGIVSHS+IIGLSLGVS SPCT
Sbjct: 241 IGHAHAHGHGHGHGHGHVHGGLDAVNGARHIVVSQVLELGIVSHSIIIGLSLGVSQSPCT 300

Query: 301 IRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNP 360
           IRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA TTP+GI IG A++S++N 
Sbjct: 301 IRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPIGIGIGTAVASSFNS 360

Query: 361 NSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS 412
           +S GALV EGILDSLSAGILVYMALVDLIAADFLS +M CNFRLQIVSY  LFLGAGLMS
Sbjct: 361 HSVGALVTEGILDSLSAGILVYMALVDLIAADFLSTKMRCNFRLQIVSYVMLFLGAGLMS 420

BLAST of CmaCh12G006400 vs. ExPASy Swiss-Prot
Match: O04089 (Zinc transporter 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ZIP4 PE=2 SV=1)

HSP 1 Score: 492.7 bits (1267), Expect = 4.2e-138
Identity = 264/376 (70.21%), Postives = 297/376 (78.99%), Query Frame = 0

Query: 40  CSVSELELCRDDSAALNFKLIAIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFA 99
           C   E +LCRDDSAA   K +AIASIL +G  G+AIPL GR+RRFL+T+GNLFVA KAFA
Sbjct: 10  CDAGESDLCRDDSAAFLLKFVAIASILLAGAAGVAIPLIGRNRRFLQTEGNLFVAAKAFA 69

Query: 100 AGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYER 159
           AGVILAT FVHML  G++ALS+PCLP+FPWSKFPF GFFAM+A+L TLLVDF+GTQYYER
Sbjct: 70  AGVILATGFVHMLAGGTEALSNPCLPDFPWSKFPFPGFFAMVAALATLLVDFMGTQYYER 129

Query: 160 KQGQIRLKEETNRVGSGLDHGL-EAGILPAADSNV---KVFGEEDGGGMHIVGMHAHAAQ 219
           KQ       E N+  +    G  E  ++P     V   KVFGEEDGGG+HIVG+ AHAA 
Sbjct: 130 KQ-------ERNQAATEAAAGSEEIAVVPVVGERVTDNKVFGEEDGGGIHIVGIRAHAAH 189

Query: 220 HRHSHPQGKEACDGHVTLHDHAHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGL 279
           HRHSH      CDGH   H H+HGH HG    D E+G RHVVVSQILELGIVSHS+IIGL
Sbjct: 190 HRHSHSNSHGTCDGHA--HGHSHGHMHG--NSDVENGARHVVVSQILELGIVSHSIIIGL 249

Query: 280 SLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAI 339
           SLGVS SPCTIRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA TTP+GI I
Sbjct: 250 SLGVSQSPCTIRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPLGIGI 309

Query: 340 GAAISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYC 399
           G A++S++N +S GALV EGILDSLSAGILVYMALVDLIAADFLSKRMSCN RLQ+VSY 
Sbjct: 310 GTAVASSFNSHSPGALVTEGILDSLSAGILVYMALVDLIAADFLSKRMSCNLRLQVVSYV 369

Query: 400 TLFLGAGLMSLLALWA 412
            LFLGAGLMS LA+WA
Sbjct: 370 MLFLGAGLMSALAIWA 374

BLAST of CmaCh12G006400 vs. ExPASy Swiss-Prot
Match: Q5Z653 (Zinc transporter 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP10 PE=3 SV=2)

HSP 1 Score: 413.7 bits (1062), Expect = 2.5e-114
Identity = 235/401 (58.60%), Postives = 274/401 (68.33%), Query Frame = 0

Query: 28  VRTVSESMGNSSCSV------SELELCRDDSAALNFKLIAIASILTSGVFGIAIPLFGRH 87
           +R ++ S+  +SC         + E CRD++AAL  K++A+A+IL +G  G+AIPL GR 
Sbjct: 13  IRQIAASVSAASCDAVVGGGGDKDEECRDEAAALRLKMVAVAAILIAGAAGVAIPLVGRR 72

Query: 88  RR-------FLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPF 147
           RR          + G LFV  KAFAAGVILAT FVHML D   ALS+PCLP  PW +FPF
Sbjct: 73  RRGGGGGGGGGASSGGLFVLAKAFAAGVILATGFVHMLHDAEHALSNPCLPHSPWRRFPF 132

Query: 148 SGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHGLEAGILPAADSNVK 207
            GF AM+A+L TL+VDFVGT +YERK  Q           + L    + G LP  D    
Sbjct: 133 PGFVAMLAALATLVVDFVGTHFYERKHRQEEAAAAAEEAAAALLE--DGGALPVGDGE-- 192

Query: 208 VFGEEDGGG----MHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGHSHGLDGDDDE 267
             G +  GG    MHIVG+HAHAA HRHSH     AC G      HAHGH H   G ++ 
Sbjct: 193 --GRDGRGGKRDAMHIVGIHAHAAAHRHSHAHVHGACHGGAVNDAHAHGHGH---GHEEG 252

Query: 268 SGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQ 327
              RHVVVSQILELGIVSHSVIIGLSLGVS SPCTI+PL+AALSFHQFFEGFALGGCIS+
Sbjct: 253 PSARHVVVSQILELGIVSHSVIIGLSLGVSQSPCTIKPLVAALSFHQFFEGFALGGCISE 312

Query: 328 AQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSLSAGILVYMAL 387
           AQ    S  LMA FFA TTP GI +GAA++S YNPNS  ALV EGILDS+SAGIL+YMAL
Sbjct: 313 AQLKNFSAFLMAFFFAITTPAGITVGAAVASFYNPNSPRALVVEGILDSMSAGILIYMAL 372

Query: 388 VDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           VDLIAADFLS++MSCN RLQ+ SY  LFLGA  M+ LALWA
Sbjct: 373 VDLIAADFLSRKMSCNPRLQVGSYIALFLGAMAMAALALWA 404

BLAST of CmaCh12G006400 vs. ExPASy Swiss-Prot
Match: O82643 (Zinc transporter 9 OS=Arabidopsis thaliana OX=3702 GN=ZIP9 PE=2 SV=1)

HSP 1 Score: 401.0 bits (1029), Expect = 1.6e-110
Identity = 222/357 (62.18%), Postives = 265/357 (74.23%), Query Frame = 0

Query: 62  IASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALSD 121
           +ASIL SG  G++IPL G     L  +G L    KAFAAGVILAT FVHML  GSKALSD
Sbjct: 1   MASILISGAAGVSIPLVG---TLLPLNGGLMRGAKAFAAGVILATGFVHMLSGGSKALSD 60

Query: 122 PCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGL---- 181
           PCLPEFPW  FPF  FFAM+A+LLTLL DF+ T YYERKQ ++ + +    +G+ +    
Sbjct: 61  PCLPEFPWKMFPFPEFFAMVAALLTLLADFMITGYYERKQEKM-MNQSVESLGTQVSVMS 120

Query: 182 DHGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHS---HPQGKEACDGHVTLH 241
           D GLE+G L           +EDGG +HIVGM AHA  HRHS     +G EA      + 
Sbjct: 121 DPGLESGFLR---------DQEDGGALHIVGMRAHAEHHRHSLSMGAEGFEALSKRSGVS 180

Query: 242 DHAHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 301
            H HGHSHG      +SGVRHVVVSQILE+GIVSHS+IIG+SLGVSHSPCTIRPL+ ALS
Sbjct: 181 GHGHGHSHGHGDVGLDSGVRHVVVSQILEMGIVSHSIIIGISLGVSHSPCTIRPLLLALS 240

Query: 302 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAE 361
           FHQFFEGFALGGC+++A+     +++MA FFA TTP+G+A+G AI+S+YN  S  ALVAE
Sbjct: 241 FHQFFEGFALGGCVAEARLTPRGSAMMAFFFAITTPIGVAVGTAIASSYNSYSVAALVAE 300

Query: 362 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           G+LDSLSAGILVYMALVDLIAADFLSK+MS +FR+Q+VSYC LFLGAG+MS LA+WA
Sbjct: 301 GVLDSLSAGILVYMALVDLIAADFLSKKMSVDFRVQVVSYCFLFLGAGMMSALAIWA 344

BLAST of CmaCh12G006400 vs. ExPASy Swiss-Prot
Match: Q6L8F7 (Zinc transporter 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP7 PE=2 SV=1)

HSP 1 Score: 362.8 bits (930), Expect = 5.0e-99
Identity = 211/371 (56.87%), Postives = 249/371 (67.12%), Query Frame = 0

Query: 48  CRDDSAALNFKLIAIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATA 107
           CRDD+AAL  K +A+A+IL +GV G+ +PL GR RR L+TD   FVA KAFAAGVILAT 
Sbjct: 36  CRDDAAALRLKGVAMATILVAGVVGVGLPLAGRKRRALRTDSAAFVAAKAFAAGVILATG 95

Query: 108 FVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLK 167
           FVHML D   ALS PCLP  PW  FPF GF AM A+L TL++DF+ T++YE K      +
Sbjct: 96  FVHMLHDAEHALSSPCLPAHPWRSFPFPGFVAMSAALATLVLDFLATRFYEGKH-----R 155

Query: 168 EETNRVGSGLDHGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEAC 227
            ET RV +     L A      D  V    E+D      + +  H+  H HSHP      
Sbjct: 156 AETERVKAAAAAALAASSASDDDITVVTVTEDDNDNKAPL-LQPHS--HSHSHP------ 215

Query: 228 DGHVTLHDHAHGHSHGLDGDDDESG-------VRHVVVSQILELGIVSHSVIIGLSLGVS 287
                   H HGH H L   +   G       VR VVVSQILE+GIVSHSVIIGLSLGVS
Sbjct: 216 --------HGHGHGHELAQPEGSGGEGEVPAQVRSVVVSQILEMGIVSHSVIIGLSLGVS 275

Query: 288 HSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAIS 347
            SPCTIRPL+AALSFHQFFEGFALGGCI+QAQF TLS ++MA FFA TTP GIA GA ++
Sbjct: 276 RSPCTIRPLVAALSFHQFFEGFALGGCIAQAQFKTLSAAIMACFFAITTPAGIAAGAGVA 335

Query: 348 STYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLG 407
           S YN NS  ALV EGILDS+SAGIL+YM+LVDLIAADFL  +M+ + R Q+++Y  LFLG
Sbjct: 336 SFYNANSPRALVVEGILDSVSAGILIYMSLVDLIAADFLGGKMTGSTRQQVMAYIALFLG 384

Query: 408 AGLMSLLALWA 412
           A  MS LA+WA
Sbjct: 396 ALSMSSLAIWA 384

BLAST of CmaCh12G006400 vs. ExPASy TrEMBL
Match: A0A6J1KKC0 (zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111496508 PE=3 SV=1)

HSP 1 Score: 798.1 bits (2060), Expect = 1.7e-227
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI
Sbjct: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60

Query: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH 240
           LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH
Sbjct: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH 240

Query: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300
           SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE
Sbjct: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300

Query: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360
           GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL
Sbjct: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360

Query: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA
Sbjct: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 411

BLAST of CmaCh12G006400 vs. ExPASy TrEMBL
Match: A0A6J1GGQ1 (zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111454070 PE=3 SV=1)

HSP 1 Score: 789.6 bits (2038), Expect = 6.1e-225
Identity = 407/411 (99.03%), Postives = 408/411 (99.27%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI
Sbjct: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60

Query: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV GIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLD G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH 240
           LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGK+ACDGHVTLHDH HGH
Sbjct: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDHGHGH 240

Query: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300
           SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE
Sbjct: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300

Query: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360
           GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL
Sbjct: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360

Query: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA
Sbjct: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 411

BLAST of CmaCh12G006400 vs. ExPASy TrEMBL
Match: A0A6J1HFN1 (zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC111462610 PE=3 SV=1)

HSP 1 Score: 713.4 bits (1840), Expect = 5.5e-202
Identity = 368/414 (88.89%), Postives = 383/414 (92.51%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           M F E+LWQML  +GFQD+ R+LSES++RTVSESMGNS C VSELELCRDDSAA +FKLI
Sbjct: 1   MFFLENLWQMLCVQGFQDKARVLSESLIRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV GIAIPLFG  RRFLKTDGNLFV  KAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVTAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG IR KEE NRVGS  D G
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVEDSG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDH---A 240
           LE+GILPA D NVKVFGEEDGG MHI+GMHAHAA HRHSHPQGKEACDGHV +HDH    
Sbjct: 181 LESGILPAGDLNVKVFGEEDGGAMHIIGMHAHAAHHRHSHPQGKEACDGHVRVHDHGHGG 240

Query: 241 HGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQ 300
           HGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQ
Sbjct: 241 HGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQ 300

Query: 301 FFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGIL 360
           FFEGFALGGCISQAQFNTLSTSLMAVFFA TTP+GIAIG A++STYNPNSTGAL+AEGIL
Sbjct: 301 FFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGTAVASTYNPNSTGALIAEGIL 360

Query: 361 DSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           DSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS LALWA
Sbjct: 361 DSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 414

BLAST of CmaCh12G006400 vs. ExPASy TrEMBL
Match: A0A0A0LKS6 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351690 PE=3 SV=1)

HSP 1 Score: 711.8 bits (1836), Expect = 1.6e-201
Identity = 368/417 (88.25%), Postives = 388/417 (93.05%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           M FFEDL QML   GFQD+TRLLSES +RTVSESM NS+C VSELELCR+DSAAL+FKLI
Sbjct: 1   MFFFEDLLQMLCIRGFQDKTRLLSESFIRTVSESMANSACGVSELELCRNDSAALHFKLI 60

Query: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILT+GV GIA+PLFG+ RRFLKTDGNLFVA KAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTAGVIGIALPLFGKQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG IR KEE NRVGS +D G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGIIRSKEEANRVGSLMDTG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHD----- 240
           LE+GI+PA D NVKVFGEE+GGGMHIVGMHAHAA HRH+HPQGKEACDGH+ +HD     
Sbjct: 181 LESGIVPAGDLNVKVFGEEEGGGMHIVGMHAHAAHHRHNHPQGKEACDGHMGVHDHGHGH 240

Query: 241 -HAHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300
            H HGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS
Sbjct: 241 AHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 300

Query: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAE 360
           FHQFFEGFALGGCISQAQFNTLSTSLMAVFFA TTP+GIA+GAA++STYNPNSTGALVAE
Sbjct: 301 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPLGIAVGAAVASTYNPNSTGALVAE 360

Query: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQ+VSYCTLFLGAGLMS LALWA
Sbjct: 361 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQLVSYCTLFLGAGLMSSLALWA 417

BLAST of CmaCh12G006400 vs. ExPASy TrEMBL
Match: A0A6J1K6U3 (zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC111491704 PE=3 SV=1)

HSP 1 Score: 708.4 bits (1827), Expect = 1.8e-200
Identity = 366/416 (87.98%), Postives = 383/416 (92.07%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           M F EDLWQ+L  +GFQD+ R+LSES++RTVSESMGNS C VSELELCRDDSAA +FKLI
Sbjct: 1   MFFLEDLWQILCVQGFQDKARVLSESLLRTVSESMGNSDCGVSELELCRDDSAAFHFKLI 60

Query: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV GIAIPLFG  RRFLKTDGNLFVA KAFAAGVILATAFVHMLPDGS ALS
Sbjct: 61  AIASILTSGVIGIAIPLFGSQRRFLKTDGNLFVAAKAFAAGVILATAFVHMLPDGSNALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180
           DPCLP+FPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQG IR KEE NRVGS +D G
Sbjct: 121 DPCLPKFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGVIRSKEEENRVGSVVDSG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDH---- 240
           LE+GILPA D NVK+FGEEDGG MHIVGMHAHA  HR SHPQGKEACDGHV +HDH    
Sbjct: 181 LESGILPAGDLNVKLFGEEDGGAMHIVGMHAHAGHHRPSHPQGKEACDGHVRVHDHGHAH 240

Query: 241 -AHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300
             HGHSHGLDGDDDESGVRHVV+SQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF
Sbjct: 241 GGHGHSHGLDGDDDESGVRHVVISQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSF 300

Query: 301 HQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEG 360
           HQFFEGFALGGCISQAQFNTLSTSLMAVFFA TTP+GIAIGAA++STYNPNSTGAL+AEG
Sbjct: 301 HQFFEGFALGGCISQAQFNTLSTSLMAVFFAITTPIGIAIGAAVASTYNPNSTGALIAEG 360

Query: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS LALWA
Sbjct: 361 ILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSSLALWA 416

BLAST of CmaCh12G006400 vs. NCBI nr
Match: XP_023002737.1 (zinc transporter 4, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 798.1 bits (2060), Expect = 3.5e-227
Identity = 411/411 (100.00%), Postives = 411/411 (100.00%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI
Sbjct: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60

Query: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH 240
           LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH
Sbjct: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH 240

Query: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300
           SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE
Sbjct: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300

Query: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360
           GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL
Sbjct: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360

Query: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA
Sbjct: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 411

BLAST of CmaCh12G006400 vs. NCBI nr
Match: XP_022951131.1 (zinc transporter 4, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 789.6 bits (2038), Expect = 1.3e-224
Identity = 407/411 (99.03%), Postives = 408/411 (99.27%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI
Sbjct: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60

Query: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV GIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLD G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH 240
           LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGK+ACDGHVTLHDH HGH
Sbjct: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDHGHGH 240

Query: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300
           SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE
Sbjct: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300

Query: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360
           GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL
Sbjct: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360

Query: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA
Sbjct: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 411

BLAST of CmaCh12G006400 vs. NCBI nr
Match: KAG6585744.1 (Fe(2+) transport protein 3, chloroplastic, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 788.1 bits (2034), Expect = 3.7e-224
Identity = 406/411 (98.78%), Postives = 407/411 (99.03%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI
Sbjct: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60

Query: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV GIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLD G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH 240
           LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGK+ACDGHVTLHDH HGH
Sbjct: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDHGHGH 240

Query: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300
           SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE
Sbjct: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300

Query: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360
           GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIG AISSTYNPNSTGALVAEGILDSL
Sbjct: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGTAISSTYNPNSTGALVAEGILDSL 360

Query: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA
Sbjct: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 411

BLAST of CmaCh12G006400 vs. NCBI nr
Match: XP_023538392.1 (zinc transporter 4, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 787.7 bits (2033), Expect = 4.8e-224
Identity = 405/411 (98.54%), Postives = 408/411 (99.27%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60
           MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI
Sbjct: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSSCSVSELELCRDDSAALNFKLI 60

Query: 61  AIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120
           AIASILTSGV GIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS
Sbjct: 61  AIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALS 120

Query: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDHG 180
           DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLD G
Sbjct: 121 DPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGLDSG 180

Query: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTLHDHAHGH 240
           LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGK+ACDGHVTLHDH HGH
Sbjct: 181 LEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKDACDGHVTLHDHGHGH 240

Query: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300
           SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE
Sbjct: 241 SHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFE 300

Query: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAEGILDSL 360
           GFALGGCISQAQFNTLSTSLMAVFFAATTP+GIAIGAAISSTYNPNS+GALVAEGILDSL
Sbjct: 301 GFALGGCISQAQFNTLSTSLMAVFFAATTPIGIAIGAAISSTYNPNSSGALVAEGILDSL 360

Query: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA
Sbjct: 361 SAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 411

BLAST of CmaCh12G006400 vs. NCBI nr
Match: KAG7020655.1 (Zinc transporter 4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 722.6 bits (1864), Expect = 1.9e-204
Identity = 372/377 (98.67%), Postives = 373/377 (98.94%), Query Frame = 0

Query: 35  MGNSSCSVSELELCRDDSAALNFKLIAIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVA 94
           MGNSSCSVSELELCRDDSAALNFKLIAIASILTSGV GIAIPLFGRHRRFLKTDGNLFVA
Sbjct: 1   MGNSSCSVSELELCRDDSAALNFKLIAIASILTSGVLGIAIPLFGRHRRFLKTDGNLFVA 60

Query: 95  TKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGT 154
           TKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGT
Sbjct: 61  TKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGT 120

Query: 155 QYYERKQGQIRLKEETNRVGSGLDHGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAA 214
           QYYERKQGQIRLKEETNRVGSGLD GLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAA
Sbjct: 121 QYYERKQGQIRLKEETNRVGSGLDSGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAA 180

Query: 215 QHRHSHPQGKEACDGHVTLHDHAHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIG 274
           QHRHSHPQGK+ACDGHVTLHDH HGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIG
Sbjct: 181 QHRHSHPQGKDACDGHVTLHDHGHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIG 240

Query: 275 LSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIA 334
           LSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIA
Sbjct: 241 LSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIA 300

Query: 335 IGAAISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSY 394
           IG AISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSY
Sbjct: 301 IGTAISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSY 360

Query: 395 CTLFLGAGLMSLLALWA 412
           CTLFLGAGLMSLLALWA
Sbjct: 361 CTLFLGAGLMSLLALWA 377

BLAST of CmaCh12G006400 vs. TAIR 10
Match: AT1G10970.1 (zinc transporter 4 precursor )

HSP 1 Score: 507.3 bits (1305), Expect = 1.2e-143
Identity = 277/419 (66.11%), Postives = 320/419 (76.37%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLSESIVRTVSESMGNSS----CSVSELELCRDDSAALN 60
           M+F + LW++     F      LSESI++ + E+M +S+    C   E +LCRDDSAA  
Sbjct: 1   MIFVDVLWKLFPLYSFGSGRDSLSESILQIIPETMASSTTKILCDAGESDLCRDDSAAFL 60

Query: 61  FKLIAIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGS 120
            K +AIASIL +G  G+AIPL GR+RRFL+T+GNLFVA KAFAAGVILAT FVHML  G+
Sbjct: 61  LKFVAIASILLAGAAGVAIPLIGRNRRFLQTEGNLFVAAKAFAAGVILATGFVHMLAGGT 120

Query: 121 KALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSG 180
           +ALS+PCLP+FPWSKFPF GFFAM+A+L TLLVDF+GTQYYERKQ       E N+  + 
Sbjct: 121 EALSNPCLPDFPWSKFPFPGFFAMVAALATLLVDFMGTQYYERKQ-------ERNQAATE 180

Query: 181 LDHGL-EAGILPAADSNV---KVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVT 240
              G  E  ++P     V   KVFGEEDGGG+HIVG+ AHAA HRHSH      CDGH  
Sbjct: 181 AAAGSEEIAVVPVVGERVTDNKVFGEEDGGGIHIVGIRAHAAHHRHSHSNSHGTCDGHA- 240

Query: 241 LHDHAHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAA 300
            H H+HGH HG    D E+G RHVVVSQILELGIVSHS+IIGLSLGVS SPCTIRPLIAA
Sbjct: 241 -HGHSHGHMHG--NSDVENGARHVVVSQILELGIVSHSIIIGLSLGVSQSPCTIRPLIAA 300

Query: 301 LSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALV 360
           LSFHQFFEGFALGGCISQAQF   S ++MA FFA TTP+GI IG A++S++N +S GALV
Sbjct: 301 LSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPLGIGIGTAVASSFNSHSPGALV 360

Query: 361 AEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
            EGILDSLSAGILVYMALVDLIAADFLSKRMSCN RLQ+VSY  LFLGAGLMS LA+WA
Sbjct: 361 TEGILDSLSAGILVYMALVDLIAADFLSKRMSCNLRLQVVSYVMLFLGAGLMSALAIWA 408

BLAST of CmaCh12G006400 vs. TAIR 10
Match: AT1G60960.1 (iron regulated transporter 3 )

HSP 1 Score: 506.1 bits (1302), Expect = 2.6e-143
Identity = 273/426 (64.08%), Postives = 324/426 (76.06%), Query Frame = 0

Query: 1   MLFFEDLWQMLWFEGFQDQTRLLS--ESIVRTVSESMGNSS----CSVSELELCRDDSAA 60
           M F + LW+++    F  +T+ LS  ESI++ V E+M  +S    C+ SE +LCRDDSAA
Sbjct: 1   MFFVDVLWKLVPLYLFGSETKSLSATESILQIVPEAMAATSSNVLCNASESDLCRDDSAA 60

Query: 61  LNFKLIAIASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPD 120
              K +AIASIL +G  G+ IPL GR+RRFL+TDGNLFV  KAFAAGVILAT FVHML  
Sbjct: 61  FLLKFVAIASILLAGAAGVTIPLIGRNRRFLQTDGNLFVTAKAFAAGVILATGFVHMLAG 120

Query: 121 GSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVG 180
           G++AL +PCLP+FPWSKFPF GFFAM+A+L+TL VDF+GTQYYERKQ +    E     G
Sbjct: 121 GTEALKNPCLPDFPWSKFPFPGFFAMIAALITLFVDFMGTQYYERKQ-EREASESVEPFG 180

Query: 181 SGLDHGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHSHPQGKEACDGHVTL- 240
                G+   ++    ++ KVFGEED GG+HIVG+HAHAA HRHSHP G ++C+GH  + 
Sbjct: 181 REQSPGIVVPMIGEGTNDGKVFGEEDSGGIHIVGIHAHAAHHRHSHPPGHDSCEGHSKID 240

Query: 241 --HDHAHGHSHG------LDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCT 300
             H HAHGH HG        G D  +G RH+VVSQ+LELGIVSHS+IIGLSLGVS SPCT
Sbjct: 241 IGHAHAHGHGHGHGHGHVHGGLDAVNGARHIVVSQVLELGIVSHSIIIGLSLGVSQSPCT 300

Query: 301 IRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNP 360
           IRPLIAALSFHQFFEGFALGGCISQAQF   S ++MA FFA TTP+GI IG A++S++N 
Sbjct: 301 IRPLIAALSFHQFFEGFALGGCISQAQFRNKSATIMACFFALTTPIGIGIGTAVASSFNS 360

Query: 361 NSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMS 412
           +S GALV EGILDSLSAGILVYMALVDLIAADFLS +M CNFRLQIVSY  LFLGAGLMS
Sbjct: 361 HSVGALVTEGILDSLSAGILVYMALVDLIAADFLSTKMRCNFRLQIVSYVMLFLGAGLMS 420

BLAST of CmaCh12G006400 vs. TAIR 10
Match: AT4G33020.1 (ZIP metal ion transporter family )

HSP 1 Score: 401.0 bits (1029), Expect = 1.2e-111
Identity = 222/357 (62.18%), Postives = 265/357 (74.23%), Query Frame = 0

Query: 62  IASILTSGVFGIAIPLFGRHRRFLKTDGNLFVATKAFAAGVILATAFVHMLPDGSKALSD 121
           +ASIL SG  G++IPL G     L  +G L    KAFAAGVILAT FVHML  GSKALSD
Sbjct: 1   MASILISGAAGVSIPLVG---TLLPLNGGLMRGAKAFAAGVILATGFVHMLSGGSKALSD 60

Query: 122 PCLPEFPWSKFPFSGFFAMMASLLTLLVDFVGTQYYERKQGQIRLKEETNRVGSGL---- 181
           PCLPEFPW  FPF  FFAM+A+LLTLL DF+ T YYERKQ ++ + +    +G+ +    
Sbjct: 61  PCLPEFPWKMFPFPEFFAMVAALLTLLADFMITGYYERKQEKM-MNQSVESLGTQVSVMS 120

Query: 182 DHGLEAGILPAADSNVKVFGEEDGGGMHIVGMHAHAAQHRHS---HPQGKEACDGHVTLH 241
           D GLE+G L           +EDGG +HIVGM AHA  HRHS     +G EA      + 
Sbjct: 121 DPGLESGFLR---------DQEDGGALHIVGMRAHAEHHRHSLSMGAEGFEALSKRSGVS 180

Query: 242 DHAHGHSHGLDGDDDESGVRHVVVSQILELGIVSHSVIIGLSLGVSHSPCTIRPLIAALS 301
            H HGHSHG      +SGVRHVVVSQILE+GIVSHS+IIG+SLGVSHSPCTIRPL+ ALS
Sbjct: 181 GHGHGHSHGHGDVGLDSGVRHVVVSQILEMGIVSHSIIIGISLGVSHSPCTIRPLLLALS 240

Query: 302 FHQFFEGFALGGCISQAQFNTLSTSLMAVFFAATTPVGIAIGAAISSTYNPNSTGALVAE 361
           FHQFFEGFALGGC+++A+     +++MA FFA TTP+G+A+G AI+S+YN  S  ALVAE
Sbjct: 241 FHQFFEGFALGGCVAEARLTPRGSAMMAFFFAITTPIGVAVGTAIASSYNSYSVAALVAE 300

Query: 362 GILDSLSAGILVYMALVDLIAADFLSKRMSCNFRLQIVSYCTLFLGAGLMSLLALWA 412
           G+LDSLSAGILVYMALVDLIAADFLSK+MS +FR+Q+VSYC LFLGAG+MS LA+WA
Sbjct: 301 GVLDSLSAGILVYMALVDLIAADFLSKKMSVDFRVQVVSYCFLFLGAGMMSALAIWA 344

BLAST of CmaCh12G006400 vs. TAIR 10
Match: AT1G05300.1 (zinc transporter 5 precursor )

HSP 1 Score: 284.3 bits (726), Expect = 1.6e-76
Identity = 168/385 (43.64%), Postives = 235/385 (61.04%), Query Frame = 0

Query: 31  VSESMGNSSCSVSELELCRDDSAALNFKLIAIASILTSGVFGIAIPLFGRHRRFLKTDGN 90
           ++ S G S C  S  +   + + A  +K+ AI S+L +GV G+  PL G+    LK +  
Sbjct: 22  IAVSAGESKCECSHEDDEANKAGAKKYKIAAIPSVLAAGVIGVMFPLLGKFFPSLKPETT 81

Query: 91  LFVATKAFAAGVILATAFVHMLPDGSKALSDPCLPEFPWSKFPFSGFFAMMASLLTLLVD 150
            F  TKAFAAGVILAT F+H+LP+G + L+ PCL    W +FPF+GF AM+A++LTL VD
Sbjct: 82  FFFVTKAFAAGVILATGFMHVLPEGYEKLTSPCLKGEAW-EFPFTGFIAMVAAILTLSVD 141

Query: 151 FVGTQYYERKQGQIRLKEETNRVGSGLDHGLEAGILPAADSNVKVFGEEDGGGMHIVGMH 210
              T Y+ +        + + R+G G +     G                GGG   +G+H
Sbjct: 142 SFATSYFHKAH-----FKTSKRIGDGEEQDAGGG----------------GGGGDELGLH 201

Query: 211 AHAAQHRHSHPQGKEACDGHVTLHDHAHGHSHGLDG-DDDESGV---RHVVVSQILELGI 270
                                    HAHGH+HG+ G +  ES V   R  VV+Q+LE+GI
Sbjct: 202 V------------------------HAHGHTHGIVGVESGESQVQLHRTRVVAQVLEVGI 261

Query: 271 VSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFA 330
           + HSV+IG+SLG S SP T + L AAL FHQ FEG  LGGCI+Q  FN +S ++M++FF+
Sbjct: 262 IVHSVVIGISLGASQSPDTAKALFAALMFHQCFEGLGLGGCIAQGNFNCMSITIMSIFFS 321

Query: 331 ATTPVGIAIGAAISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCN 390
            TTPVGIA+G AISS+Y+ +S  AL+ +G+L++ SAGIL+YM+LVD +AADF+  +M  N
Sbjct: 322 VTTPVGIAVGMAISSSYDDSSPTALIVQGVLNAASAGILIYMSLVDFLAADFMHPKMQSN 360

Query: 391 FRLQIVSYCTLFLGAGLMSLLALWA 412
            RLQI+++ +L +GAG+MSLLA WA
Sbjct: 382 TRLQIMAHISLLVGAGVMSLLAKWA 360

BLAST of CmaCh12G006400 vs. TAIR 10
Match: AT5G62160.1 (zinc transporter 12 precursor )

HSP 1 Score: 266.2 bits (679), Expect = 4.5e-71
Identity = 167/385 (43.38%), Postives = 227/385 (58.96%), Query Frame = 0

Query: 31  VSESMGNSSCSVSELELCRDDSAALNFKLIAIASILTSGVFGIAIPLFGRHRRFLKTDGN 90
           VS +   + C  S+     + ++AL +K+IA  SIL +GVFG+ +P+FG     LKT+ N
Sbjct: 23  VSAAEEENQCGGSKGGSAAEKASALKYKIIAFFSILIAGVFGVCLPIFG-----LKTESN 82

Query: 91  LFVATKAFAAGVILATAFVHMLPDGSKALSDPCL-PEFPWSKFPFSGFFAMMASLLTLLV 150
            F+  KAFAAGVILAT FVH+LPD +++L+  CL  E PW  FP +G  AM AS+LT+L+
Sbjct: 83  FFMYVKAFAAGVILATGFVHILPDATESLTSSCLGEEPPWGDFPMTGLVAMAASILTMLI 142

Query: 151 DFVGTQYYERKQGQIRLKEETNRVGSGLDHGLEAGILPAADSNVKVFGEEDGGGMHIVGM 210
           +   + Y  R     RL +             E   LP     V   GEE+    H    
Sbjct: 143 ESFASGYLNRS----RLAK-------------EGKTLP-----VSTGGEEEHA--HTGSA 202

Query: 211 HAHAAQHRHSHPQGKEACDGHVTLHDHAHGHSHG---LDGDDDESGVRHVVVSQILELGI 270
           H HA+Q                       GHSHG   +  DDD   +R  +V+QILELGI
Sbjct: 203 HTHASQ-----------------------GHSHGSLLIPQDDDHIDMRKKIVTQILELGI 262

Query: 271 VSHSVIIGLSLGVSHSPCTIRPLIAALSFHQFFEGFALGGCISQAQFNTLSTSLMAVFFA 330
           V HSVIIG+SLG S S  TI+PLIAA++FHQ FEGF LGGCIS+A+F      +M +FFA
Sbjct: 263 VVHSVIIGISLGASPSVSTIKPLIAAITFHQLFEGFGLGGCISEAKFRVKKIWVMLMFFA 322

Query: 331 ATTPVGIAIGAAISSTYNPNSTGALVAEGILDSLSAGILVYMALVDLIAADFLSKRMSCN 390
            T P+GI IG  ++  YN NS  AL   G L++ ++GIL+YMALVDL+A  F++++   +
Sbjct: 323 LTAPIGIGIGIGVAEIYNENSPMALKVSGFLNATASGILIYMALVDLVAPLFMNQKTQSS 355

Query: 391 FRLQIVSYCTLFLGAGLMSLLALWA 412
            ++Q+    +L +GAGLMSLLA+WA
Sbjct: 383 MKIQVACSVSLVVGAGLMSLLAIWA 355

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q8LE593.6e-14264.08Fe(2+) transport protein 3, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=IRT... [more]
O040894.2e-13870.21Zinc transporter 4, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ZIP4 PE=2 S... [more]
Q5Z6532.5e-11458.60Zinc transporter 10 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP10 PE=3 SV=2[more]
O826431.6e-11062.18Zinc transporter 9 OS=Arabidopsis thaliana OX=3702 GN=ZIP9 PE=2 SV=1[more]
Q6L8F75.0e-9956.87Zinc transporter 7 OS=Oryza sativa subsp. japonica OX=39947 GN=ZIP7 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KKC01.7e-227100.00zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114965... [more]
A0A6J1GGQ16.1e-22599.03zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1HFN15.5e-20288.89zinc transporter 4, chloroplastic-like OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
A0A0A0LKS61.6e-20188.25Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G351690 PE=3 SV=1[more]
A0A6J1K6U31.8e-20087.98zinc transporter 4, chloroplastic-like OS=Cucurbita maxima OX=3661 GN=LOC1114917... [more]
Match NameE-valueIdentityDescription
XP_023002737.13.5e-227100.00zinc transporter 4, chloroplastic-like [Cucurbita maxima][more]
XP_022951131.11.3e-22499.03zinc transporter 4, chloroplastic-like [Cucurbita moschata][more]
KAG6585744.13.7e-22498.78Fe(2+) transport protein 3, chloroplastic, partial [Cucurbita argyrosperma subsp... [more]
XP_023538392.14.8e-22498.54zinc transporter 4, chloroplastic-like [Cucurbita pepo subsp. pepo][more]
KAG7020655.11.9e-20498.67Zinc transporter 4, chloroplastic, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT1G10970.11.2e-14366.11zinc transporter 4 precursor [more]
AT1G60960.12.6e-14364.08iron regulated transporter 3 [more]
AT4G33020.11.2e-11162.18ZIP metal ion transporter family [more]
AT1G05300.11.6e-7643.64zinc transporter 5 precursor [more]
AT5G62160.14.5e-7143.38zinc transporter 12 precursor [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003689Zinc/iron permeasePFAMPF02535Zipcoord: 56..408
e-value: 4.0E-77
score: 259.7
IPR004698Zinc/iron permease, fungal/plantTIGRFAMTIGR00820TIGR00820coord: 40..411
e-value: 7.2E-133
score: 440.6
NoneNo IPR availablePANTHERPTHR11040ZINC/IRON TRANSPORTERcoord: 35..411
NoneNo IPR availablePANTHERPTHR11040:SF157ZIP ZINC/IRON TRANSPORT FAMILY PROTEINcoord: 35..411

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G006400.1CmaCh12G006400.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0071577 zinc ion transmembrane transport
biological_process GO:0030001 metal ion transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
cellular_component GO:0016020 membrane
molecular_function GO:0005385 zinc ion transmembrane transporter activity
molecular_function GO:0046873 metal ion transmembrane transporter activity