CmaCh12G006160 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh12G006160 vs. ExPASy Swiss-Prot
Match: O48651 (Squalene monooxygenase SE1 OS=Panax ginseng OX=4054 GN=SQE1 PE=2 SV=1)

HSP 1 Score: 806.6 bits (2082), Expect = 1.7e-232
Identity = 390/529 (73.72%), Postives = 453/529 (85.63%), Query Frame = 0

Query: 1   MMVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPED 60
           ++++    GWI A + G ++ + F     R+  +  +    + D       +NG   PE+
Sbjct: 22  LLIDQYFLGWIFAFLFGFLLLLNF----KRKREKNNSTEFGTDD-------SNGYYTPEN 81

Query: 61  ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELG 120
                DVIIVGAGVAGSALA+TL  DGRRVHVIERDLTE DRIVGE LQPGG+LKLIELG
Sbjct: 82  IAGSTDVIIVGAGVAGSALAYTLANDGRRVHVIERDLTEQDRIVGELLQPGGYLKLIELG 141

Query: 121 LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP 180
           L DCV EIDAQ+++GYAL+ DGKNTR+SYPL  FHSDV+GRSFHNGRF+QRMREKAASLP
Sbjct: 142 LEDCVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHSDVAGRSFHNGRFVQRMREKAASLP 201

Query: 181 NVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240
           NVR+EQGTVTSL+EKKG++KGVQYK+K+G+E +A+APLTIVCDGCFSNLRRSLC P V+V
Sbjct: 202 NVRMEQGTVTSLVEKKGSVKGVQYKTKDGQELSAFAPLTIVCDGCFSNLRRSLCNPKVEV 261

Query: 241 PSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDME 300
           PSCFVGL+LEN  LP  NHGHV+L DPSPILFY ISSTEIRCLVDVPGQKVP I NG++ 
Sbjct: 262 PSCFVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPCISNGELA 321

Query: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360
            YLKTVVAPQVP Q++++FIAA++KGNIRTMPNRSMPA PHPTPGALL+GDAFNMRHPLT
Sbjct: 322 NYLKTVVAPQVPKQLYNSFIAAVDKGNIRTMPNRSMPADPHPTPGALLLGDAFNMRHPLT 381

Query: 361 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFC 420
           GGGMTVALSDIV++R+LL+PL+DL+D+ TLCKYLES+YTLRKPVASTINTLAGALYKVFC
Sbjct: 382 GGGMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGALYKVFC 441

Query: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLP 480
           ASPD+AR+EMR ACFDYLSLGG+ S GPI+LLSGLNPRP  L LHFFAVAIYGVGRLL+P
Sbjct: 442 ASPDKARQEMRNACFDYLSLGGICSQGPIALLSGLNPRPISLFLHFFAVAIYGVGRLLIP 501

Query: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVH 530
           FPS K +W+GARLI  ASGII PIIK+EG+RQMFFPA VPAYYR+PP+H
Sbjct: 502 FPSPKRMWLGARLILGASGIIFPIIKSEGLRQMFFPAIVPAYYRAPPIH 539

BLAST of CmaCh12G006160 vs. ExPASy Swiss-Prot
Match: B7TWW5 (Squalene monooxygenase SE2 OS=Panax ginseng OX=4054 GN=SQE2 PE=2 SV=2)

HSP 1 Score: 798.9 bits (2062), Expect = 3.5e-230
Identity = 395/535 (73.83%), Postives = 450/535 (84.11%), Query Frame = 0

Query: 4   EHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCV------ISPAIANGECE 63
           E+  Y  + AA L     +  F    RR RR       S + +       S +  NG C 
Sbjct: 9   ENDEYFLMFAATLLFGFVLYLFTLRRRRRRREKKGGAGSMEIINGAYKMTSSSEVNGHCT 68

Query: 64  PED-ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKL 123
           PED A +  DVIIVGAGVAGSALA+TL KDGRRVHVIERDLTE DRIVGE LQPGG+LKL
Sbjct: 69  PEDIAGSSDDVIIVGAGVAGSALAYTLAKDGRRVHVIERDLTEQDRIVGELLQPGGYLKL 128

Query: 124 IELGLGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKA 183
           +ELGL DCV EIDAQ+++GYAL+ DGKNTR+SYPL  FH+DV+GRSFHNGRFIQRMREKA
Sbjct: 129 VELGLEDCVNEIDAQRVFGYALYMDGKNTRLSYPLEKFHADVAGRSFHNGRFIQRMREKA 188

Query: 184 ASLPNVRLEQGTVTSLLEKKGTIKGVQYKSKNGEE-KTAYAPLTIVCDGCFSNLRRSLCK 243
           ASLPNVR+EQGTVTSL+E+KGT+KGV+YK+KNG+E   AYAPLTIVCDGCFSNLR SLC 
Sbjct: 189 ASLPNVRMEQGTVTSLVEQKGTVKGVRYKTKNGQEMSAAYAPLTIVCDGCFSNLRHSLCN 248

Query: 244 PMVDVPSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIP 303
           P VDVPSCFVGL+LEN  LP  NHGHV+L DPSPILFY ISSTEIRCLVDVPGQKVPSI 
Sbjct: 249 PKVDVPSCFVGLILENIDLPHINHGHVILADPSPILFYKISSTEIRCLVDVPGQKVPSIA 308

Query: 304 NGDMEKYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNM 363
           NG++  YLKT VAPQ+PP+++ +FIAAI+KG I+TMPNRSMPA PH TPGALL+GDAFNM
Sbjct: 309 NGELAHYLKTSVAPQIPPELYKSFIAAIDKGKIKTMPNRSMPADPHSTPGALLLGDAFNM 368

Query: 364 RHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGAL 423
           RHPLTGGGMTVALSDIV++R+LL+PL+DL+D+ TLCKYLES+YTLRKPVASTINTLAGAL
Sbjct: 369 RHPLTGGGMTVALSDIVLIRDLLRPLRDLHDSSTLCKYLESFYTLRKPVASTINTLAGAL 428

Query: 424 YKVFCASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVG 483
           YKVFCASPD+AR+EMR ACFDYLSLGG+ S GPI+LLSGLNPRP  L  HFFAVAIYGVG
Sbjct: 429 YKVFCASPDKARQEMRDACFDYLSLGGICSEGPIALLSGLNPRPMSLFFHFFAVAIYGVG 488

Query: 484 RLLLPFPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHK 531
           RLL+PFPS + +W+GARLI  ASGII PIIK+EGVRQMFFPATVPAYYR+PP+ K
Sbjct: 489 RLLIPFPSPRKMWLGARLISGASGIIFPIIKSEGVRQMFFPATVPAYYRAPPITK 543

BLAST of CmaCh12G006160 vs. ExPASy Swiss-Prot
Match: Q9SM02 (Squalene epoxidase 1 OS=Arabidopsis thaliana OX=3702 GN=SQE1 PE=1 SV=1)

HSP 1 Score: 779.6 bits (2012), Expect = 2.2e-224
Identity = 383/530 (72.26%), Postives = 445/530 (83.96%), Query Frame = 0

Query: 3   VEHCAYGWIL-----AAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECE 62
           +E   + WIL     + ++  V    FFV   R   R    + ++    +      G   
Sbjct: 1   MESQLWNWILPLLISSLLISFVAFYGFFVKPKRNGLRHDRKTVSTVTSDVGSVNITG--- 60

Query: 63  PEDADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLI 122
               D  ADVI+VGAGVAGSALA+TLGKD RRVHVIERDL+EPDRIVGE LQPGG+LKL+
Sbjct: 61  ----DTVADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLL 120

Query: 123 ELGLGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAA 182
           ELG+ DCVEEIDAQ++YGYALFK+GK  R++YPL  FH DVSGRSFHNGRFIQRMREKAA
Sbjct: 121 ELGIEDCVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAA 180

Query: 183 SLPNVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPM 242
           SLPNV+LEQGTV SLLE+ GTIKGV+YK+K GEE+TA+A LTIVCDGCFSNLRRSLC P 
Sbjct: 181 SLPNVQLEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQ 240

Query: 243 VDVPSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNG 302
           V+VPSCFVGLVLENC LP+ANHGHVVL DPSPIL YPISSTE+RCLVDVPGQKVPSI NG
Sbjct: 241 VEVPSCFVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANG 300

Query: 303 DMEKYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRH 362
           +M+ YLKTVVAPQ+P +++D+FIAA++KGNI++MPNRSMPA+P+PTPGALLMGDAFNMRH
Sbjct: 301 EMKNYLKTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRH 360

Query: 363 PLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYK 422
           PLTGGGMTVAL+DIVVLRNLL+PL+DL+D  +LCKYLES+YTLRKPVA+TINTLA ALY+
Sbjct: 361 PLTGGGMTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQ 420

Query: 423 VFCASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRL 482
           VFC+S ++AR EMR+ACFDYL LGG+ ++GP+SLLSGLNPRP  LV HFFAVA+YGV RL
Sbjct: 421 VFCSSENEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRL 480

Query: 483 LLPFPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPP 528
           L+PFPS K IW+GA+LI  ASGII PIIKAEGVRQMFFPATVPAYY   P
Sbjct: 481 LIPFPSPKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of CmaCh12G006160 vs. ExPASy Swiss-Prot
Match: Q8VYH2 (Squalene epoxidase 3 OS=Arabidopsis thaliana OX=3702 GN=SQE3 PE=1 SV=1)

HSP 1 Score: 773.1 bits (1995), Expect = 2.1e-222
Identity = 371/464 (79.96%), Postives = 422/464 (90.95%), Query Frame = 0

Query: 64  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELGLGD 123
           D D+IIVGAGVAG+ALAHTLGK+GRRVHVIERDLTEPDRIVGE LQPGG+LKLIELGL D
Sbjct: 54  DVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 113

Query: 124 CVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLPNVR 183
           CV++IDAQ++ GYALFKDGK+T++SYPL  F SDV+GRSFHNGRF+QRMREKA+ LPNVR
Sbjct: 114 CVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRFVQRMREKASLLPNVR 173

Query: 184 LEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 243
           +EQGTVTSL+E+ G IKGVQYK+K+G+E  ++APLTIVCDGCFSNLRRSLCKP V+VPS 
Sbjct: 174 MEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSNLRRSLCKPKVEVPSN 233

Query: 244 FVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDMEKYL 303
           FVGLVLENC LPF NHGHVVLGDPSPILFYPISS+E+RCLVDVPG K+PS+ +G+M  +L
Sbjct: 234 FVGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPGSKLPSVASGEMAHHL 293

Query: 304 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGG 363
           KT+VAPQVPPQI DAFI+A+EKGNIRTMPNRSMPA P  TPGALL+GDAFNMRHPLTGGG
Sbjct: 294 KTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALLLGDAFNMRHPLTGGG 353

Query: 364 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFCASP 423
           MTVALSDIV+LR+LL PL DL +  +L KY+ES+YTLRKPVASTINTLAGALYKVF ASP
Sbjct: 354 MTVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTINTLAGALYKVFLASP 413

Query: 424 DQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLPFPS 483
           D AR EMR+ACFDYLSLGGV S+GP++LLSGLNPRP  LVLHFFAVAI+GVGRLL+P PS
Sbjct: 414 DDARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLPS 473

Query: 484 LKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPP 528
           +K +W+GARLI SASGII PIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 474 VKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

BLAST of CmaCh12G006160 vs. ExPASy Swiss-Prot
Match: O81000 (Squalene epoxidase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SQE2 PE=1 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 8.1e-219
Identity = 370/495 (74.75%), Postives = 426/495 (86.06%), Query Frame = 0

Query: 33  RRGGTDSTASKDCVISPAIANGECEPEDADADADVIIVGAGVAGSALAHTLGKDGRRVHV 92
           RR    +  ++  V+S      +    + D+  DVIIVGAGVAGSALAHTLGK+GRRVHV
Sbjct: 91  RRSSNKNKKNRGLVVSQNDTVSKNLETEVDSGTDVIIVGAGVAGSALAHTLGKEGRRVHV 150

Query: 93  IERDLTEPDRIVGEFLQPGGHLKLIELGLGDCVEEIDAQKLYGYALFKDGKNTRVSYPLG 152
           IERD +E DRIVGE LQPGG+LKLIELGL DCV++IDAQ++ GY LFKDGK+T+++YPL 
Sbjct: 151 IERDFSEQDRIVGELLQPGGYLKLIELGLEDCVKKIDAQRVLGYVLFKDGKHTKLAYPLE 210

Query: 153 NFHSDVSGRSFHNGRFIQRMREKAASLPNVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEK 212
            F SDV+GRSFHNGRF+QRMREKA +L NVRLEQGTVTSLLE+ GTIKGV+Y++K G E 
Sbjct: 211 TFDSDVAGRSFHNGRFVQRMREKALTLSNVRLEQGTVTSLLEEHGTIKGVRYRTKEGNEF 270

Query: 213 TAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCFVGLVLENCRLPFANHGHVVLGDPSPILF 272
            ++APLTIVCDGCFSNLRRSLCKP VDVPS FVGLVLENC LPFANHGHVVLGDPSPIL 
Sbjct: 271 RSFAPLTIVCDGCFSNLRRSLCKPKVDVPSTFVGLVLENCELPFANHGHVVLGDPSPILM 330

Query: 273 YPISSTEIRCLVDVPGQKVPSIPNGDMEKYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMP 332
           YPISS+E+RCLVDVPGQK+P I NG+M KYLKT VAPQVP ++ +AFI A+EKGNIRTMP
Sbjct: 331 YPISSSEVRCLVDVPGQKLPPIANGEMAKYLKTRVAPQVPTKVREAFITAVEKGNIRTMP 390

Query: 333 NRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCK 392
           NRSMPA P PTPGALL+GDAFNMRHPLTGGGMTVAL+DIVVLR+LL+P+++LND   L K
Sbjct: 391 NRSMPADPIPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPIRNLNDKEALSK 450

Query: 393 YLESYYTLRKPVASTINTLAGALYKVFCASPDQARKEMRQACFDYLSLGGVFSNGPISLL 452
           Y+ES+YTLRKPVASTINTLA ALYKVF AS D+AR EMR+ACFDYLSLGGVFS+GP++LL
Sbjct: 451 YIESFYTLRKPVASTINTLADALYKVFLASSDEARTEMREACFDYLSLGGVFSSGPVALL 510

Query: 453 SGLNPRPSCLVLHFFAVAIYGVGRLLLPFPSLKGIWIGARLIYSASGIILPIIKAEGVRQ 512
           SGLNPRP  LVLHFFAVAIY V RL+LPFPS++  W+GAR+I SAS II PIIKAEGVRQ
Sbjct: 511 SGLNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFWLGARIISSASSIIFPIIKAEGVRQ 570

Query: 513 MFFPATVPAYYRSPP 528
           MFFP T+PA YR+PP
Sbjct: 571 MFFPRTIPAIYRAPP 585

BLAST of CmaCh12G006160 vs. ExPASy TrEMBL
Match: A0A6J1KU20 (Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111496410 PE=3 SV=1)

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 533/533 (100.00%), Postives = 533/533 (100.00%), Query Frame = 0

Query: 1   MMVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPED 60
           MMVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPED
Sbjct: 1   MMVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPED 60

Query: 61  ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELG 120
           ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELG
Sbjct: 61  ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELG 120

Query: 121 LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP 180
           LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP
Sbjct: 121 LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP 180

Query: 181 NVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240
           NVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV
Sbjct: 181 NVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240

Query: 241 PSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDME 300
           PSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDME
Sbjct: 241 PSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDME 300

Query: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360
           KYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT
Sbjct: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360

Query: 361 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFC 420
           GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFC
Sbjct: 361 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFC 420

Query: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLP 480
           ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLP
Sbjct: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLP 480

Query: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT 534
           FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT
Sbjct: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT 533

BLAST of CmaCh12G006160 vs. ExPASy TrEMBL
Match: A0A6J1GH98 (Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111454180 PE=3 SV=1)

HSP 1 Score: 1036.6 bits (2679), Expect = 3.7e-299
Identity = 512/533 (96.06%), Postives = 522/533 (97.94%), Query Frame = 0

Query: 1   MMVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPED 60
           MMV+HCA+ WIL  VLGLVVAVTFFVA PRRNRRGGTDSTASKDCVIS AIANGEC+P+ 
Sbjct: 1   MMVDHCAFAWILDVVLGLVVAVTFFVAAPRRNRRGGTDSTASKDCVISTAIANGECKPD- 60

Query: 61  ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELG 120
            DADA+VIIVGAGVAGSALA+TLGKDGRRVHVIERDLTEPDRIVGEFLQPGG+LKLIELG
Sbjct: 61  -DADAEVIIVGAGVAGSALAYTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGYLKLIELG 120

Query: 121 LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP 180
           LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP
Sbjct: 121 LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP 180

Query: 181 NVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240
           NVRLEQGTVTSLLE KGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV
Sbjct: 181 NVRLEQGTVTSLLETKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240

Query: 241 PSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDME 300
           PSCFVGLVLENC+LPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSI NGDME
Sbjct: 241 PSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGDME 300

Query: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360
           KYLKTVVAPQVPPQIHDAFIAAIEKGN+RTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT
Sbjct: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNVRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360

Query: 361 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFC 420
           GGGMTVALSDIVVLRNLLKPLKDLNDA TLCKYLES+YTLRKPVASTINTLAGALYKVFC
Sbjct: 361 GGGMTVALSDIVVLRNLLKPLKDLNDASTLCKYLESFYTLRKPVASTINTLAGALYKVFC 420

Query: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLP 480
           ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPS LVLHFFAVAIYGVGRLLLP
Sbjct: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSSLVLHFFAVAIYGVGRLLLP 480

Query: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT 534
           FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT
Sbjct: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT 531

BLAST of CmaCh12G006160 vs. ExPASy TrEMBL
Match: A0A6J1K5U9 (Squalene monooxygenase OS=Cucurbita maxima OX=3661 GN=LOC111491959 PE=3 SV=1)

HSP 1 Score: 969.9 bits (2506), Expect = 4.3e-279
Identity = 478/531 (90.02%), Postives = 501/531 (94.35%), Query Frame = 0

Query: 2   MVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPEDA 61
           MV++CA+GWILAAVLGL +A++FFV+ PRRNRRGG DST   + V S +  NGEC     
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVS-PRRNRRGGADSTPRSEGVRSSSTTNGEC--RSV 60

Query: 62  DADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELGL 121
           D DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGE LQPGG+LKLIELGL
Sbjct: 61  DGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 120

Query: 122 GDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLPN 181
            DCVEEIDAQK+YGYALFKDGKNT++SYPL  F SDVSGRSFHNGRFIQRMREKAASLPN
Sbjct: 121 QDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPN 180

Query: 182 VRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 241
           VRLEQGTVTSLLE+KGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP
Sbjct: 181 VRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 240

Query: 242 SCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDMEK 301
           SCFVGLVLENC+LPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQK+PSI NG+MEK
Sbjct: 241 SCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEK 300

Query: 302 YLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTG 361
           YLKT+VAPQVPPQIHDAFIAAI+KGNIRTMPNRSMPAAP PTPGALLMGDAFNMRHPLTG
Sbjct: 301 YLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTG 360

Query: 362 GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFCA 421
           GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLES+YTLRKPVASTINTLAGALYKVFCA
Sbjct: 361 GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCA 420

Query: 422 SPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLPF 481
           SPDQARKEMRQACFDYLSLGG+FSNGP+SLLSGLNPRP  LVLHFFAVAIYGVGRLLLPF
Sbjct: 421 SPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPF 480

Query: 482 PSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPI 533
           PS KGIWIGARL+YSASGII PIIKAEGVRQMFFPATVPAYYRSPPVHK I
Sbjct: 481 PSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKSI 528

BLAST of CmaCh12G006160 vs. ExPASy TrEMBL
Match: A0A6J1HEG7 (Squalene monooxygenase OS=Cucurbita moschata OX=3662 GN=LOC111462746 PE=3 SV=1)

HSP 1 Score: 964.1 bits (2491), Expect = 2.3e-277
Identity = 477/531 (89.83%), Postives = 500/531 (94.16%), Query Frame = 0

Query: 2   MVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPEDA 61
           MV++CA+GWILAAVLGL +A++FFV+ PRRNRRGG DST   + V S +  NGEC     
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVS-PRRNRRGGADSTPRSEGVRSSSTTNGEC--RSV 60

Query: 62  DADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELGL 121
           D DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGE LQPGG+LKLIELGL
Sbjct: 61  DCDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 120

Query: 122 GDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLPN 181
            DCVEEIDAQK+YGYALFKDGKNT++SYPL  F SDVSGRSFHNGRFIQRMREKAASLPN
Sbjct: 121 QDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPN 180

Query: 182 VRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 241
           VRLEQGTVTSLLE+KGTIKGVQYKSKNGEEKTA+APLTIVCDGCFSNLRRSLCKPMVDVP
Sbjct: 181 VRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAHAPLTIVCDGCFSNLRRSLCKPMVDVP 240

Query: 242 SCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDMEK 301
           SCFVGLVLENC+LPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSI NG+MEK
Sbjct: 241 SCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEK 300

Query: 302 YLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTG 361
           YLKT+VAPQVPPQIHDAFIAAI+KGNIRTMPNRSMPAAP PTPGALLMGDAFNMRHPLTG
Sbjct: 301 YLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTG 360

Query: 362 GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFCA 421
           GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLES+YTLRKPVASTINTLAGALYKVFCA
Sbjct: 361 GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCA 420

Query: 422 SPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLPF 481
           SPDQARKEMRQACFDYLSLGG+FSNGP+SLLSGLNPRP  LVLHFFAVAIYGVGRLLLPF
Sbjct: 421 SPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPF 480

Query: 482 PSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPI 533
           PS KGIWIGARL+YSASGII PIIKAEGVRQMFFPATVPAYYRSPPV K I
Sbjct: 481 PSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTI 528

BLAST of CmaCh12G006160 vs. ExPASy TrEMBL
Match: A0A345Z0U8 (Squalene monooxygenase OS=Cucurbita pepo OX=3663 PE=2 SV=1)

HSP 1 Score: 963.0 bits (2488), Expect = 5.2e-277
Identity = 477/531 (89.83%), Postives = 499/531 (93.97%), Query Frame = 0

Query: 2   MVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPEDA 61
           MV++CA+GWILAAVLGL +A++FFV+ PRRNRRGG DST   + VIS +  NGEC     
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVS-PRRNRRGGADSTPRSEGVISSSATNGEC--RSV 60

Query: 62  DADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELGL 121
           D DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGE LQPGG+LKLIELGL
Sbjct: 61  DGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 120

Query: 122 GDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLPN 181
            DCVEEIDAQK+YGYALFKDGKNT++SYPL  F SDVSGRSFHNGRFIQRMREKAASLPN
Sbjct: 121 QDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPN 180

Query: 182 VRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 241
           VRLEQGTVTSLLE+KGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP
Sbjct: 181 VRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 240

Query: 242 SCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDMEK 301
           SCFVGLVLENC+LPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSI NG+MEK
Sbjct: 241 SCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEK 300

Query: 302 YLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTG 361
           YLKT+VAPQVPPQIHDAFIAAI+KGNIRTMPNRSMPAAP PTPGALLMGDAFNMRHPLTG
Sbjct: 301 YLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTG 360

Query: 362 GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFCA 421
           GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYL S+YTLRKPVASTINTLAGALYKVFCA
Sbjct: 361 GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLGSFYTLRKPVASTINTLAGALYKVFCA 420

Query: 422 SPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLPF 481
           SPDQARKEMRQACFDYLSLGG+FSNGP+SLLSGLNPRP  LVLHFFAVAIYGVGRLLLPF
Sbjct: 421 SPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPF 480

Query: 482 PSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPI 533
           PS KGIWIGARL+YSAS II PIIKAEGVRQMFFPATVPAYYRSPPV K I
Sbjct: 481 PSPKGIWIGARLVYSASRIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTI 528

BLAST of CmaCh12G006160 vs. NCBI nr
Match: XP_023002598.1 (squalene monooxygenase-like [Cucurbita maxima])

HSP 1 Score: 1082.8 bits (2799), Expect = 0.0e+00
Identity = 533/533 (100.00%), Postives = 533/533 (100.00%), Query Frame = 0

Query: 1   MMVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPED 60
           MMVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPED
Sbjct: 1   MMVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPED 60

Query: 61  ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELG 120
           ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELG
Sbjct: 61  ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELG 120

Query: 121 LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP 180
           LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP
Sbjct: 121 LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP 180

Query: 181 NVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240
           NVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV
Sbjct: 181 NVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240

Query: 241 PSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDME 300
           PSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDME
Sbjct: 241 PSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDME 300

Query: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360
           KYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT
Sbjct: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360

Query: 361 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFC 420
           GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFC
Sbjct: 361 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFC 420

Query: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLP 480
           ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLP
Sbjct: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLP 480

Query: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT 534
           FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT
Sbjct: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT 533

BLAST of CmaCh12G006160 vs. NCBI nr
Match: XP_022951307.1 (squalene monooxygenase-like [Cucurbita moschata])

HSP 1 Score: 1036.6 bits (2679), Expect = 7.6e-299
Identity = 512/533 (96.06%), Postives = 522/533 (97.94%), Query Frame = 0

Query: 1   MMVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPED 60
           MMV+HCA+ WIL  VLGLVVAVTFFVA PRRNRRGGTDSTASKDCVIS AIANGEC+P+ 
Sbjct: 1   MMVDHCAFAWILDVVLGLVVAVTFFVAAPRRNRRGGTDSTASKDCVISTAIANGECKPD- 60

Query: 61  ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELG 120
            DADA+VIIVGAGVAGSALA+TLGKDGRRVHVIERDLTEPDRIVGEFLQPGG+LKLIELG
Sbjct: 61  -DADAEVIIVGAGVAGSALAYTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGYLKLIELG 120

Query: 121 LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP 180
           LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP
Sbjct: 121 LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP 180

Query: 181 NVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240
           NVRLEQGTVTSLLE KGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV
Sbjct: 181 NVRLEQGTVTSLLETKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240

Query: 241 PSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDME 300
           PSCFVGLVLENC+LPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSI NGDME
Sbjct: 241 PSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGDME 300

Query: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360
           KYLKTVVAPQVPPQIHDAFIAAIEKGN+RTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT
Sbjct: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNVRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360

Query: 361 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFC 420
           GGGMTVALSDIVVLRNLLKPLKDLNDA TLCKYLES+YTLRKPVASTINTLAGALYKVFC
Sbjct: 361 GGGMTVALSDIVVLRNLLKPLKDLNDASTLCKYLESFYTLRKPVASTINTLAGALYKVFC 420

Query: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLP 480
           ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPS LVLHFFAVAIYGVGRLLLP
Sbjct: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSSLVLHFFAVAIYGVGRLLLP 480

Query: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT 534
           FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT
Sbjct: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT 531

BLAST of CmaCh12G006160 vs. NCBI nr
Match: KAG7020623.1 (hypothetical protein SDJN02_17309, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1024.2 bits (2647), Expect = 3.9e-295
Identity = 508/533 (95.31%), Postives = 518/533 (97.19%), Query Frame = 0

Query: 1   MMVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPED 60
           MMV+HCA+ WIL  VLGLVVAVTFFVA PRRNRRGGTDSTASKDCVIS AIANGEC+P+ 
Sbjct: 1   MMVDHCAFAWILDVVLGLVVAVTFFVAAPRRNRRGGTDSTASKDCVISTAIANGECKPD- 60

Query: 61  ADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELG 120
            DADADVIIVGAGVAGSALA+TLGKDGRRVHVIERDLTEPDRIVGEFLQPGG+LKLIELG
Sbjct: 61  -DADADVIIVGAGVAGSALAYTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGYLKLIELG 120

Query: 121 LGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLP 180
           LGDCVEEIDAQKL GYALFKDGKNTRVSYPLGNFHSDV+GRSFHNGRFIQRMREKAASLP
Sbjct: 121 LGDCVEEIDAQKLCGYALFKDGKNTRVSYPLGNFHSDVTGRSFHNGRFIQRMREKAASLP 180

Query: 181 NVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240
           NVRLEQGTVTSLLE KGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV
Sbjct: 181 NVRLEQGTVTSLLETKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDV 240

Query: 241 PSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDME 300
           PSCFVGLVLENC+LPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSI NGDME
Sbjct: 241 PSCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGDME 300

Query: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360
           KYLKTVVAPQVPPQIHDAFIAAIEKGN+RTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT
Sbjct: 301 KYLKTVVAPQVPPQIHDAFIAAIEKGNVRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLT 360

Query: 361 GGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFC 420
           GGGMTVALSDIVVLRNLLKPLKDLNDA TLCKYLES+YTLRKPVASTINTLAGALYKVFC
Sbjct: 361 GGGMTVALSDIVVLRNLLKPLKDLNDASTLCKYLESFYTLRKPVASTINTLAGALYKVFC 420

Query: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLP 480
           ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPS LVLHFFAVAIYGVGRLLL 
Sbjct: 421 ASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSSLVLHFFAVAIYGVGRLLLS 480

Query: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPIT 534
           FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPV KP T
Sbjct: 481 FPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVLKPTT 531

BLAST of CmaCh12G006160 vs. NCBI nr
Match: XP_022996831.1 (squalene monooxygenase-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 969.9 bits (2506), Expect = 8.8e-279
Identity = 478/531 (90.02%), Postives = 501/531 (94.35%), Query Frame = 0

Query: 2   MVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPEDA 61
           MV++CA+GWILAAVLGL +A++FFV+ PRRNRRGG DST   + V S +  NGEC     
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVS-PRRNRRGGADSTPRSEGVRSSSTTNGEC--RSV 60

Query: 62  DADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELGL 121
           D DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGE LQPGG+LKLIELGL
Sbjct: 61  DGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 120

Query: 122 GDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLPN 181
            DCVEEIDAQK+YGYALFKDGKNT++SYPL  F SDVSGRSFHNGRFIQRMREKAASLPN
Sbjct: 121 QDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPN 180

Query: 182 VRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 241
           VRLEQGTVTSLLE+KGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP
Sbjct: 181 VRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 240

Query: 242 SCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDMEK 301
           SCFVGLVLENC+LPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQK+PSI NG+MEK
Sbjct: 241 SCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKIPSISNGEMEK 300

Query: 302 YLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTG 361
           YLKT+VAPQVPPQIHDAFIAAI+KGNIRTMPNRSMPAAP PTPGALLMGDAFNMRHPLTG
Sbjct: 301 YLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTG 360

Query: 362 GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFCA 421
           GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLES+YTLRKPVASTINTLAGALYKVFCA
Sbjct: 361 GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCA 420

Query: 422 SPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLPF 481
           SPDQARKEMRQACFDYLSLGG+FSNGP+SLLSGLNPRP  LVLHFFAVAIYGVGRLLLPF
Sbjct: 421 SPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPF 480

Query: 482 PSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPI 533
           PS KGIWIGARL+YSASGII PIIKAEGVRQMFFPATVPAYYRSPPVHK I
Sbjct: 481 PSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVHKSI 528

BLAST of CmaCh12G006160 vs. NCBI nr
Match: XP_023546518.1 (squalene monooxygenase-like isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 968.8 bits (2503), Expect = 2.0e-278
Identity = 479/531 (90.21%), Postives = 501/531 (94.35%), Query Frame = 0

Query: 2   MVEHCAYGWILAAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECEPEDA 61
           MV++CA+GWILAAVLGL +A++FFV+ PRRNRRGG DST   + VIS +  NGEC     
Sbjct: 1   MVDYCAFGWILAAVLGLAIALSFFVS-PRRNRRGGADSTPRSEGVISSSATNGEC--RSV 60

Query: 62  DADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELGL 121
           D DADVIIVGAGVAGSALAHTLGKDGR VHVIERDLTEPDRIVGE LQPGG+LKLIELGL
Sbjct: 61  DGDADVIIVGAGVAGSALAHTLGKDGRLVHVIERDLTEPDRIVGELLQPGGYLKLIELGL 120

Query: 122 GDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLPN 181
            DCVEEIDAQK+YGYALFKDGKNT++SYPL  F SDVSGRSFHNGRFIQRMREKAASLPN
Sbjct: 121 QDCVEEIDAQKVYGYALFKDGKNTQLSYPLEKFQSDVSGRSFHNGRFIQRMREKAASLPN 180

Query: 182 VRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 241
           VRLEQGTVTSLLE+KGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP
Sbjct: 181 VRLEQGTVTSLLEEKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVP 240

Query: 242 SCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDMEK 301
           SCFVGLVLENC+LPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSI NG+MEK
Sbjct: 241 SCFVGLVLENCQLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSISNGEMEK 300

Query: 302 YLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTG 361
           YLKT+VAPQVPPQIHDAFIAAI+KGNIRTMPNRSMPAAP PTPGALLMGDAFNMRHPLTG
Sbjct: 301 YLKTIVAPQVPPQIHDAFIAAIDKGNIRTMPNRSMPAAPQPTPGALLMGDAFNMRHPLTG 360

Query: 362 GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFCA 421
           GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLES+YTLRKPVASTINTLAGALYKVFCA
Sbjct: 361 GGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESFYTLRKPVASTINTLAGALYKVFCA 420

Query: 422 SPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLPF 481
           SPDQARKEMRQACFDYLSLGG+FSNGP+SLLSGLNPRP  LVLHFFAVAIYGVGRLLLPF
Sbjct: 421 SPDQARKEMRQACFDYLSLGGIFSNGPVSLLSGLNPRPLSLVLHFFAVAIYGVGRLLLPF 480

Query: 482 PSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPPVHKPI 533
           PS KGIWIGARL+YSASGII PIIKAEGVRQMFFPATVPAYYRSPPV K I
Sbjct: 481 PSPKGIWIGARLVYSASGIIFPIIKAEGVRQMFFPATVPAYYRSPPVLKTI 528

BLAST of CmaCh12G006160 vs. TAIR 10
Match: AT1G58440.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 779.6 bits (2012), Expect = 1.6e-225
Identity = 383/530 (72.26%), Postives = 445/530 (83.96%), Query Frame = 0

Query: 3   VEHCAYGWIL-----AAVLGLVVAVTFFVAVPRRNRRGGTDSTASKDCVISPAIANGECE 62
           +E   + WIL     + ++  V    FFV   R   R    + ++    +      G   
Sbjct: 1   MESQLWNWILPLLISSLLISFVAFYGFFVKPKRNGLRHDRKTVSTVTSDVGSVNITG--- 60

Query: 63  PEDADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLI 122
               D  ADVI+VGAGVAGSALA+TLGKD RRVHVIERDL+EPDRIVGE LQPGG+LKL+
Sbjct: 61  ----DTVADVIVVGAGVAGSALAYTLGKDKRRVHVIERDLSEPDRIVGELLQPGGYLKLL 120

Query: 123 ELGLGDCVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAA 182
           ELG+ DCVEEIDAQ++YGYALFK+GK  R++YPL  FH DVSGRSFHNGRFIQRMREKAA
Sbjct: 121 ELGIEDCVEEIDAQRVYGYALFKNGKRIRLAYPLEKFHEDVSGRSFHNGRFIQRMREKAA 180

Query: 183 SLPNVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPM 242
           SLPNV+LEQGTV SLLE+ GTIKGV+YK+K GEE+TA+A LTIVCDGCFSNLRRSLC P 
Sbjct: 181 SLPNVQLEQGTVLSLLEENGTIKGVRYKNKAGEEQTAFAALTIVCDGCFSNLRRSLCNPQ 240

Query: 243 VDVPSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNG 302
           V+VPSCFVGLVLENC LP+ANHGHVVL DPSPIL YPISSTE+RCLVDVPGQKVPSI NG
Sbjct: 241 VEVPSCFVGLVLENCNLPYANHGHVVLADPSPILMYPISSTEVRCLVDVPGQKVPSIANG 300

Query: 303 DMEKYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRH 362
           +M+ YLKTVVAPQ+P +++D+FIAA++KGNI++MPNRSMPA+P+PTPGALLMGDAFNMRH
Sbjct: 301 EMKNYLKTVVAPQMPHEVYDSFIAAVDKGNIKSMPNRSMPASPYPTPGALLMGDAFNMRH 360

Query: 363 PLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYK 422
           PLTGGGMTVAL+DIVVLRNLL+PL+DL+D  +LCKYLES+YTLRKPVA+TINTLA ALY+
Sbjct: 361 PLTGGGMTVALADIVVLRNLLRPLRDLSDGASLCKYLESFYTLRKPVAATINTLANALYQ 420

Query: 423 VFCASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRL 482
           VFC+S ++AR EMR+ACFDYL LGG+ ++GP+SLLSGLNPRP  LV HFFAVA+YGV RL
Sbjct: 421 VFCSSENEARNEMREACFDYLGLGGMCTSGPVSLLSGLNPRPLTLVCHFFAVAVYGVIRL 480

Query: 483 LLPFPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPP 528
           L+PFPS K IW+GA+LI  ASGII PIIKAEGVRQMFFPATVPAYY   P
Sbjct: 481 LIPFPSPKRIWLGAKLISGASGIIFPIIKAEGVRQMFFPATVPAYYYKAP 523

BLAST of CmaCh12G006160 vs. TAIR 10
Match: AT4G37760.1 (squalene epoxidase 3 )

HSP 1 Score: 773.1 bits (1995), Expect = 1.5e-223
Identity = 371/464 (79.96%), Postives = 422/464 (90.95%), Query Frame = 0

Query: 64  DADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIELGLGD 123
           D D+IIVGAGVAG+ALAHTLGK+GRRVHVIERDLTEPDRIVGE LQPGG+LKLIELGL D
Sbjct: 54  DVDIIIVGAGVAGAALAHTLGKEGRRVHVIERDLTEPDRIVGELLQPGGYLKLIELGLED 113

Query: 124 CVEEIDAQKLYGYALFKDGKNTRVSYPLGNFHSDVSGRSFHNGRFIQRMREKAASLPNVR 183
           CV++IDAQ++ GYALFKDGK+T++SYPL  F SDV+GRSFHNGRF+QRMREKA+ LPNVR
Sbjct: 114 CVKDIDAQRVLGYALFKDGKHTKLSYPLDQFDSDVAGRSFHNGRFVQRMREKASLLPNVR 173

Query: 184 LEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKPMVDVPSC 243
           +EQGTVTSL+E+ G IKGVQYK+K+G+E  ++APLTIVCDGCFSNLRRSLCKP V+VPS 
Sbjct: 174 MEQGTVTSLVEENGIIKGVQYKTKDGQELKSFAPLTIVCDGCFSNLRRSLCKPKVEVPSN 233

Query: 244 FVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPNGDMEKYL 303
           FVGLVLENC LPF NHGHVVLGDPSPILFYPISS+E+RCLVDVPG K+PS+ +G+M  +L
Sbjct: 234 FVGLVLENCELPFPNHGHVVLGDPSPILFYPISSSEVRCLVDVPGSKLPSVASGEMAHHL 293

Query: 304 KTVVAPQVPPQIHDAFIAAIEKGNIRTMPNRSMPAAPHPTPGALLMGDAFNMRHPLTGGG 363
           KT+VAPQVPPQI DAFI+A+EKGNIRTMPNRSMPA P  TPGALL+GDAFNMRHPLTGGG
Sbjct: 294 KTMVAPQVPPQIRDAFISAVEKGNIRTMPNRSMPADPIHTPGALLLGDAFNMRHPLTGGG 353

Query: 364 MTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGALYKVFCASP 423
           MTVALSDIV+LR+LL PL DL +  +L KY+ES+YTLRKPVASTINTLAGALYKVF ASP
Sbjct: 354 MTVALSDIVILRDLLNPLVDLTNKESLSKYIESFYTLRKPVASTINTLAGALYKVFLASP 413

Query: 424 DQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVGRLLLPFPS 483
           D AR EMR+ACFDYLSLGGV S+GP++LLSGLNPRP  LVLHFFAVAI+GVGRLL+P PS
Sbjct: 414 DDARSEMRRACFDYLSLGGVCSSGPVALLSGLNPRPMSLVLHFFAVAIFGVGRLLVPLPS 473

Query: 484 LKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRSPP 528
           +K +W+GARLI SASGII PIIKAEGVRQMFFP T+PA YR+PP
Sbjct: 474 VKRLWLGARLISSASGIIFPIIKAEGVRQMFFPRTIPAIYRAPP 517

BLAST of CmaCh12G006160 vs. TAIR 10
Match: AT2G22830.1 (squalene epoxidase 2 )

HSP 1 Score: 761.1 bits (1964), Expect = 5.8e-220
Identity = 370/495 (74.75%), Postives = 426/495 (86.06%), Query Frame = 0

Query: 33  RRGGTDSTASKDCVISPAIANGECEPEDADADADVIIVGAGVAGSALAHTLGKDGRRVHV 92
           RR    +  ++  V+S      +    + D+  DVIIVGAGVAGSALAHTLGK+GRRVHV
Sbjct: 91  RRSSNKNKKNRGLVVSQNDTVSKNLETEVDSGTDVIIVGAGVAGSALAHTLGKEGRRVHV 150

Query: 93  IERDLTEPDRIVGEFLQPGGHLKLIELGLGDCVEEIDAQKLYGYALFKDGKNTRVSYPLG 152
           IERD +E DRIVGE LQPGG+LKLIELGL DCV++IDAQ++ GY LFKDGK+T+++YPL 
Sbjct: 151 IERDFSEQDRIVGELLQPGGYLKLIELGLEDCVKKIDAQRVLGYVLFKDGKHTKLAYPLE 210

Query: 153 NFHSDVSGRSFHNGRFIQRMREKAASLPNVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEK 212
            F SDV+GRSFHNGRF+QRMREKA +L NVRLEQGTVTSLLE+ GTIKGV+Y++K G E 
Sbjct: 211 TFDSDVAGRSFHNGRFVQRMREKALTLSNVRLEQGTVTSLLEEHGTIKGVRYRTKEGNEF 270

Query: 213 TAYAPLTIVCDGCFSNLRRSLCKPMVDVPSCFVGLVLENCRLPFANHGHVVLGDPSPILF 272
            ++APLTIVCDGCFSNLRRSLCKP VDVPS FVGLVLENC LPFANHGHVVLGDPSPIL 
Sbjct: 271 RSFAPLTIVCDGCFSNLRRSLCKPKVDVPSTFVGLVLENCELPFANHGHVVLGDPSPILM 330

Query: 273 YPISSTEIRCLVDVPGQKVPSIPNGDMEKYLKTVVAPQVPPQIHDAFIAAIEKGNIRTMP 332
           YPISS+E+RCLVDVPGQK+P I NG+M KYLKT VAPQVP ++ +AFI A+EKGNIRTMP
Sbjct: 331 YPISSSEVRCLVDVPGQKLPPIANGEMAKYLKTRVAPQVPTKVREAFITAVEKGNIRTMP 390

Query: 333 NRSMPAAPHPTPGALLMGDAFNMRHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCK 392
           NRSMPA P PTPGALL+GDAFNMRHPLTGGGMTVAL+DIVVLR+LL+P+++LND   L K
Sbjct: 391 NRSMPADPIPTPGALLLGDAFNMRHPLTGGGMTVALADIVVLRDLLRPIRNLNDKEALSK 450

Query: 393 YLESYYTLRKPVASTINTLAGALYKVFCASPDQARKEMRQACFDYLSLGGVFSNGPISLL 452
           Y+ES+YTLRKPVASTINTLA ALYKVF AS D+AR EMR+ACFDYLSLGGVFS+GP++LL
Sbjct: 451 YIESFYTLRKPVASTINTLADALYKVFLASSDEARTEMREACFDYLSLGGVFSSGPVALL 510

Query: 453 SGLNPRPSCLVLHFFAVAIYGVGRLLLPFPSLKGIWIGARLIYSASGIILPIIKAEGVRQ 512
           SGLNPRP  LVLHFFAVAIY V RL+LPFPS++  W+GAR+I SAS II PIIKAEGVRQ
Sbjct: 511 SGLNPRPLSLVLHFFAVAIYAVCRLMLPFPSIESFWLGARIISSASSIIFPIIKAEGVRQ 570

Query: 513 MFFPATVPAYYRSPP 528
           MFFP T+PA YR+PP
Sbjct: 571 MFFPRTIPAIYRAPP 585

BLAST of CmaCh12G006160 vs. TAIR 10
Match: AT5G24150.1 (FAD/NAD(P)-binding oxidoreductase family protein )

HSP 1 Score: 493.4 bits (1269), Expect = 2.2e-139
Identity = 245/470 (52.13%), Postives = 326/470 (69.36%), Query Frame = 0

Query: 59  EDADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIE 118
           E  D   DVIIVGAGV GSALA+ L KDGRRVHVIERDL EP+RI+GEF+QPGG L L +
Sbjct: 40  EREDGATDVIIVGAGVGGSALAYALAKDGRRVHVIERDLREPERIMGEFMQPGGRLMLSK 99

Query: 119 LGLGDCVEEIDAQKLYGYALFKDGKNTRVSYPL--GNFHSDVSGRSFHNGRFIQRMREKA 178
           LGL DC+E IDAQK  G  ++KDGK    S+P+   NF  D S RSFHNGRF+QR+R+KA
Sbjct: 100 LGLEDCLEGIDAQKATGMTVYKDGKEAVASFPVDNNNFPFDPSARSFHNGRFVQRLRQKA 159

Query: 179 ASLPNVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKP 238
           +SLPNVRLE+GTV SL+E+KG IKGV YK+  GEE TA APLT+VCDGC+SNLRRSL   
Sbjct: 160 SSLPNVRLEEGTVKSLIEEKGVIKGVTYKNSAGEETTALAPLTVVCDGCYSNLRRSLNDN 219

Query: 239 MVDVPSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIPN 298
             +V S  VG + +NC+L       +++  PS  + Y ISST++RC+ +V    +PSI N
Sbjct: 220 NAEVLSYQVGFISKNCQLEEPEKLKLIMSKPSFTMLYQISSTDVRCVFEVLPNNIPSISN 279

Query: 299 GDMEKYLKTVVAPQVPPQIHDAFIAAIEKG-NIRTMPNRSMPAAPHPTPGALLMGDAFNM 358
           G+M  ++K  +APQVP ++   F+  I++G +I+ MP + M A      G +L+GDAFNM
Sbjct: 280 GEMATFVKNTIAPQVPLKLRKIFLKGIDEGEHIKAMPTKKMTATLSEKKGVILLGDAFNM 339

Query: 359 RHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGAL 418
           RHP    GM V LSDI++LR LL+PL +L +A  + + ++S+Y +RKP+++T+NTL  A 
Sbjct: 340 RHPAIASGMMVLLSDILILRRLLQPLSNLGNAQKISQVIKSFYDIRKPMSATVNTLGNAF 399

Query: 419 YKVFCASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGVG 478
            +V  AS D+A++ MRQ C+DYLS GG  ++G ++LL G+NPRP  L+ H  A+ +  +G
Sbjct: 400 SQVLVASTDEAKEAMRQGCYDYLSSGGFRTSGMMALLGGMNPRPISLIYHLCAITLSSIG 459

Query: 479 RLLLPFPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRS 526
            LL PFPS   IW   RL   A  +++P +KAEGV QM FP    AY +S
Sbjct: 460 HLLSPFPSPLRIWHSLRLFGLAMKMLVPHLKAEGVSQMLFPVNAAAYSKS 509

BLAST of CmaCh12G006160 vs. TAIR 10
Match: AT5G24160.1 (squalene monoxygenase 6 )

HSP 1 Score: 484.2 bits (1245), Expect = 1.4e-136
Identity = 239/471 (50.74%), Postives = 326/471 (69.21%), Query Frame = 0

Query: 59  EDADADADVIIVGAGVAGSALAHTLGKDGRRVHVIERDLTEPDRIVGEFLQPGGHLKLIE 118
           +  D  ADVIIVGAGV GSALA+ L KDGRRVHVIERD+ EP+R++GEF+QPGG L L +
Sbjct: 40  DQKDGAADVIIVGAGVGGSALAYALAKDGRRVHVIERDMREPERMMGEFMQPGGRLMLSK 99

Query: 119 LGLGDCVEEIDAQKLYGYALFKDGKNTRVSYPL--GNFHSDVSGRSFHNGRFIQRMREKA 178
           LGL DC+E+IDAQK  G A++KDGK     +P+   NF  + S RSFHNGRF+Q++R KA
Sbjct: 100 LGLQDCLEDIDAQKATGLAVYKDGKEADAPFPVDNNNFSYEPSARSFHNGRFVQQLRRKA 159

Query: 179 ASLPNVRLEQGTVTSLLEKKGTIKGVQYKSKNGEEKTAYAPLTIVCDGCFSNLRRSLCKP 238
            SL NVRLE+GTV SLLE+KG +KGV YK+K GEE TA APLT+VCDGC+SNLRRSL   
Sbjct: 160 FSLSNVRLEEGTVKSLLEEKGVVKGVTYKNKEGEETTALAPLTVVCDGCYSNLRRSLNDD 219

Query: 239 -MVDVPSCFVGLVLENCRLPFANHGHVVLGDPSPILFYPISSTEIRCLVDVPGQKVPSIP 298
              ++ S  VG + +NCRL      H++L  PS  + Y ISST++RC  +V  +  PSI 
Sbjct: 220 NNAEIMSYIVGYISKNCRLEEPEKLHLILSKPSFTMVYQISSTDVRCGFEVLPENFPSIA 279

Query: 299 NGDMEKYLKTVVAPQVPPQIHDAFIAAIEKG-NIRTMPNRSMPAAPHPTPGALLMGDAFN 358
           NG+M  ++K  + PQVPP++   F+  I++G +I+ +P + M +      G +++GDAFN
Sbjct: 280 NGEMSTFMKNTIVPQVPPKLRKIFLKGIDEGAHIKVVPAKRMTSTLSKKKGVIVLGDAFN 339

Query: 359 MRHPLTGGGMTVALSDIVVLRNLLKPLKDLNDAPTLCKYLESYYTLRKPVASTINTLAGA 418
           MRHP+   GM V LSDI++LR LL+PL +L DA  + + + S+Y +RKP+++T+NTL  A
Sbjct: 340 MRHPVVASGMMVLLSDILILRRLLQPLSNLGDANKVSEVINSFYDIRKPMSATVNTLGNA 399

Query: 419 LYKVFCASPDQARKEMRQACFDYLSLGGVFSNGPISLLSGLNPRPSCLVLHFFAVAIYGV 478
             +V   S D+A++ MRQ  +DYL  GG  ++G ++LL G+NPRP  LV H  A+ +  +
Sbjct: 400 FSQVLIGSTDEAKEAMRQGVYDYLCSGGFRTSGMMALLGGMNPRPLSLVYHLCAITLSSI 459

Query: 479 GRLLLPFPSLKGIWIGARLIYSASGIILPIIKAEGVRQMFFPATVPAYYRS 526
           G+LL PFPS   IW   +L   A  +++P +KAEGV QM FPA   AY++S
Sbjct: 460 GQLLSPFPSPLRIWHSLKLFGLAMKMLVPNLKAEGVSQMLFPANAAAYHKS 510