CmaCh12G006060 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G006060
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Phytosulfokine-beta
Location	Cma_Chr12: 3187104 .. 3187554 (-)
RNA-Seq Expression	CmaCh12G006060
Synteny	CmaCh12G006060

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGTCGAAGTTCATAGCTCTCTTCTCCATTGTAGCGCTCGTGACAATGTCTCTTTTCTCCGCCTCCGAAGCTCGTCCCATCGCTACATCCACCTCCACTTCCCTCGACTTCAACCATATTGCGCATCAGGTACCCTAAAATCTCATATCTCTTTGATCATGGTTTACAAGTAAGGAATACATCTCCATTGGTATAAAGATTTTAAAACAAAGCCATTCCGGAGATCCTTGATTCCTAACAATTCCATCATGAAATTAATGTTTACTCGTACTTTAATCAGGCCATTTCTGAAAAGGATCACGGCGATGTTTGCGAGGGAGGAGAGACAAAGGAGTGTCTGGCTAGAAGAACGTTAGAGGCTCATACCGATTACATCTATACCGATAATACCAAGTCATGAGCTGCTGTATTCCGACCATTATATTAATAATTATTTTTATGTGTCTCTTAT

mRNA sequence

ATGTCGAAGTTCATAGCTCTCTTCTCCATTGTAGCGCTCGTGACAATGTCTCTTTTCTCCGCCTCCGAAGCTCGTCCCATCGCTACATCCACCTCCACTTCCCTCGACTTCAACCATATTGCGCATCAGGCCATTTCTGAAAAGGATCACGGCGATGTTTGCGAGGGAGGAGAGACAAAGGAGTGTCTGGCTAGAAGAACGTTAGAGGCTCATACCGATTACATCTATACCGATAATACCAAGTCATGAGCTGCTGTATTCCGACCATTATATTAATAATTATTTTTATGTGTCTCTTAT

Coding sequence (CDS)

ATGTCGAAGTTCATAGCTCTCTTCTCCATTGTAGCGCTCGTGACAATGTCTCTTTTCTCCGCCTCCGAAGCTCGTCCCATCGCTACATCCACCTCCACTTCCCTCGACTTCAACCATATTGCGCATCAGGCCATTTCTGAAAAGGATCACGGCGATGTTTGCGAGGGAGGAGAGACAAAGGAGTGTCTGGCTAGAAGAACGTTAGAGGCTCATACCGATTACATCTATACCGATAATACCAAGTCATGA

Protein sequence

MSKFIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDVCEGGETKECLARRTLEAHTDYIYTDNTKS

Homology

[Close]

BLAST of CmaCh12G006060 vs. ExPASy Swiss-Prot
Match: Q9FS10 (Phytosulfokines OS=Asparagus officinalis OX=4686 GN=PSK PE=1 SV=1)

HSP 1 Score: 52.8 bits (125), Expect = 2.2e-06
Identity = 35/78 (44.87%), Postives = 43/78 (55.13%), Query Frame = 0


Query: 2  SKFIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDVCEGGETKE 61
          SK I L  I  L ++SL  A  ARP+  + ST       +   I EK H + CEG   +E
Sbjct: 3  SKAITLLLIALLFSLSL--AQAARPLQPADSTK------SVHVIPEKVHDEACEGVGEEE 62

Query: 62 CLARRTLEAHTDYIYTDN 80
          CL RRTL AH DYIYT +
Sbjct: 63 CLMRRTLTAHVDYIYTQD 72

[Close]

BLAST of CmaCh12G006060 vs. ExPASy Swiss-Prot
Match: Q9AR88 (Phytosulfokines 4 OS=Oryza sativa subsp. japonica OX=39947 GN=PSK4 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 1.9e-05
Identity = 36/73 (49.32%), Postives = 44/73 (60.27%), Query Frame = 0


Query: 12 ALVTMSLFSASEA------RPIATSTSTSLDFNHIAHQAISEKDHGDVCEGGE-TKECLA 71
          AL  + +F+AS A      RP  T   TSLD    A QA ++ + G  C+ GE  +ECLA
Sbjct: 11 ALAVLLIFAASSATVAMAGRPTPT---TSLD--EEAAQAAAQSEIGGGCKEGEGEEECLA 70

Query: 72 RRTLEAHTDYIYT 78
          RRTL AHTDYIYT
Sbjct: 71 RRTLTAHTDYIYT 78

[Close]

BLAST of CmaCh12G006060 vs. ExPASy Swiss-Prot
Match: Q9M2Y0 (Phytosulfokines 3 OS=Arabidopsis thaliana OX=3702 GN=PSK3 PE=2 SV=2)

HSP 1 Score: 47.0 bits (110), Expect = 1.2e-04
Identity = 29/81 (35.80%), Postives = 43/81 (53.09%), Query Frame = 0


Query: 1  MSKFIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDVCEGGETK 60
          M KF  +F +  L+  +L  A+   P   +T+T+L   +   +   +K   + C G   +
Sbjct: 1  MGKFTTIFIMALLLCSTLTYAARLTP---TTTTALSRENSVKEIEGDKVEEESCNGIGEE 60

Query: 61 ECLARRTLEAHTDYIYTDNTK 82
          ECL RR+L  HTDYIYT N K
Sbjct: 61 ECLIRRSLVLHTDYIYTQNHK 78

[Close]

BLAST of CmaCh12G006060 vs. ExPASy TrEMBL
Match: A0A6J1BQV2 (Phytosulfokine-beta OS=Momordica charantia OX=3673 GN=LOC111004777 PE=3 SV=1)

HSP 1 Score: 69.3 bits (168), Expect = 8.4e-09
Identity = 43/83 (51.81%), Postives = 57/83 (68.67%), Query Frame = 0


Query: 1   MSK-FIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDVC-EGGE 60
           MSK   ALF+I AL+TMS+  +S+ARPI T ++ S++   IAH    ++ H D C E  E
Sbjct: 22  MSKQLAALFAIAALLTMSVLLSSQARPITTFSANSVE---IAHPDSKDRVHEDGCDERAE 81

Query: 61  TKECLARRTLEAHTDYIYTDNTK 82
            ++CL RRTLEAHTDYIY+  TK
Sbjct: 82  DQDCLNRRTLEAHTDYIYSGETK 101

[Close]

BLAST of CmaCh12G006060 vs. ExPASy TrEMBL
Match: A0A6J1BSJ0 (Phytosulfokine-beta OS=Momordica charantia OX=3673 GN=LOC111005149 PE=3 SV=1)

HSP 1 Score: 62.0 bits (149), Expect = 1.3e-06
Identity = 43/81 (53.09%), Postives = 53/81 (65.43%), Query Frame = 0


Query: 1  MSKFIALFSIVALV-TMSLFSASEARP-IATSTSTSLDFNHIAHQAISEKDHGDVC-EGG 60
          M+K  ALF+IVALV TMSL ++SEARP IATS         I +    E  +GD+C E  
Sbjct: 1  MTKLAALFTIVALVLTMSLLTSSEARPTIATSVK-------IVNPVPEETVNGDICDERN 60

Query: 61 ETKECLARRTLEAHTDYIYTD 79
            ++CL RRTL AHTDYIYT+
Sbjct: 61 PDEDCLFRRTLTAHTDYIYTE 74

[Close]

BLAST of CmaCh12G006060 vs. ExPASy TrEMBL
Match: A0A2U1NSB2 (Phytosulfokine-beta OS=Artemisia annua OX=35608 GN=CTI12_AA235340 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 9.6e-05
Identity = 38/83 (45.78%), Postives = 48/83 (57.83%), Query Frame = 0


Query: 1  MSKFIALFSIVALVTMSLFSASEARPIAT----STSTSLDFNHIAHQAISEKDHGDVCEG 60
          MSK I LF IVAL+  S  S  +ARPI T    +TS       I  ++ ++ D  + CEG
Sbjct: 1  MSKAITLFLIVALLLSSTLS-YDARPIKTIPKAATSAPTTTTTIVTKSENKDDVDETCEG 60

Query: 61 GETKECLARRTLEAHTDYIYTDN 80
             +ECL RRTL AH DYIYT +
Sbjct: 61 VRKEECLTRRTLAAHLDYIYTQD 82

[Close]

BLAST of CmaCh12G006060 vs. ExPASy TrEMBL
Match: A0A118JW21 (Phytosulfokine-beta OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_004598 PE=3 SV=1)

HSP 1 Score: 55.8 bits (133), Expect = 9.6e-05
Identity = 28/72 (38.89%), Postives = 42/72 (58.33%), Query Frame = 0


Query: 10 IVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDVCEGGETKECLARRTLE 69
          ++A++ +S+ S + ARPI T    +    H   + +++    + C G   +ECL RRTL 
Sbjct: 1  MIAILLLSMLSCTVARPIPTFNDVTPIETHHTEEEVAKAKAQEKCSGPGEEECLMRRTLA 60

Query: 70 AHTDYIYTDNTK 82
          AH DYIYT NTK
Sbjct: 61 AHLDYIYTQNTK 72

[Close]

BLAST of CmaCh12G006060 vs. ExPASy TrEMBL
Match: A0A1U7X3S7 (Phytosulfokine-beta OS=Nicotiana sylvestris OX=4096 GN=LOC104230093 PE=3 SV=1)

HSP 1 Score: 54.7 bits (130), Expect = 2.1e-04
Identity = 38/79 (48.10%), Postives = 46/79 (58.23%), Query Frame = 0


Query: 1  MSKFIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDVCEGGETK 60
          MSKF ALF +  L+  SL SA  ARP   ST     F       + E D G +CEG E +
Sbjct: 1  MSKFSALFLVAILLCFSLKSA--ARP--DSTLMKAQFKDARDDKV-ENDEG-ICEGVEEE 60

Query: 61 ECLARRTLEAHTDYIYTDN 80
          +CL RRT+EAH DYIYT +
Sbjct: 61 DCLKRRTMEAHIDYIYTQS 73

[Close]

BLAST of CmaCh12G006060 vs. NCBI nr
Match: KAG6585714.1 (Phytosulfokine, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 137.9 bits (346), Expect = 4.0e-29
Identity = 68/82 (82.93%), Postives = 76/82 (92.68%), Query Frame = 0


Query: 1  MSKFIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDVCEGGETK 60
          MSK +ALFS+VALVTMSL S+SEARPIATST+TSLD NHIAHQ +SEK H DVCEGGETK
Sbjct: 1  MSKLVALFSVVALVTMSLLSSSEARPIATSTATSLDLNHIAHQVVSEKGHDDVCEGGETK 60

Query: 61 ECLARRTLEAHTDYIYTDNTKS 83
          ECLA+RT+EAHTDYIYTD+TKS
Sbjct: 61 ECLAKRTVEAHTDYIYTDDTKS 82

[Close]

BLAST of CmaCh12G006060 vs. NCBI nr
Match: XP_023538273.1 (phytosulfokines-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 130.2 bits (326), Expect = 8.2e-27
Identity = 66/82 (80.49%), Postives = 71/82 (86.59%), Query Frame = 0


Query: 1  MSKFIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDVCEGGETK 60
          MSK ++LFSIVALVTMSL S SEARPIATST+T LD NHIAHQ +SEK H DVCEGGET 
Sbjct: 1  MSKLVSLFSIVALVTMSLLSFSEARPIATSTATFLDLNHIAHQVVSEKSHDDVCEGGETM 60

Query: 61 ECLARRTLEAHTDYIYTDNTKS 83
          ECLAR T EAHTDYIYTD+TKS
Sbjct: 61 ECLARSTFEAHTDYIYTDDTKS 82

[Close]

BLAST of CmaCh12G006060 vs. NCBI nr
Match: KAG6585713.1 (Phytosulfokines 3, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 83.2 bits (204), Expect = 1.2e-12
Identity = 48/84 (57.14%), Postives = 60/84 (71.43%), Query Frame = 0


Query: 1  MSKFIALFSIVALVTMSLFSAS-EARPIATS-TSTSLDFNHIAHQAISEKDHGDVCEGGE 60
          MSK +A+F+I AL+TMS+ S+S EARPI T+ T+T LD + I HQ    K   DVC+GGE
Sbjct: 1  MSKLVAVFTIAALITMSILSSSAEARPITTTPTATPLDISQITHQ----KGREDVCKGGE 60

Query: 61 TKECLARRTLEAHTDYIYTDNTKS 83
           + CL R TLEAH+DYIYTD   S
Sbjct: 61 AEVCLVRTTLEAHSDYIYTDAINS 80

[Close]

BLAST of CmaCh12G006060 vs. NCBI nr
Match: XP_023546620.1 (phytosulfokines-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 73.2 bits (178), Expect = 1.2e-09
Identity = 47/82 (57.32%), Postives = 57/82 (69.51%), Query Frame = 0


Query: 1  MSKFIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGD-VCEGGET 60
          MSK  ALF+I AL+TMS    SEARPIAT T+TS     IA+  ++E+   D  CEG E 
Sbjct: 1  MSKLAALFTIAALLTMS----SEARPIATLTATSA---VIANPVVAEEVRKDGPCEGREK 60

Query: 61 KECLARRTLEAHTDYIYTDNTK 82
           +CL RRTLEAHTDYIY+ +TK
Sbjct: 61 DDCLIRRTLEAHTDYIYSGDTK 75

[Close]

BLAST of CmaCh12G006060 vs. NCBI nr
Match: XP_022131649.1 (uncharacterized protein LOC111004777 [Momordica charantia])

HSP 1 Score: 69.3 bits (168), Expect = 1.7e-08
Identity = 43/83 (51.81%), Postives = 57/83 (68.67%), Query Frame = 0


Query: 1   MSK-FIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDVC-EGGE 60
           MSK   ALF+I AL+TMS+  +S+ARPI T ++ S++   IAH    ++ H D C E  E
Sbjct: 22  MSKQLAALFAIAALLTMSVLLSSQARPITTFSANSVE---IAHPDSKDRVHEDGCDERAE 81

Query: 61  TKECLARRTLEAHTDYIYTDNTK 82
            ++CL RRTLEAHTDYIY+  TK
Sbjct: 82  DQDCLNRRTLEAHTDYIYSGETK 101

[Close]

BLAST of CmaCh12G006060 vs. TAIR 10
Match: AT3G49780.1 (phytosulfokine 4 precursor )

HSP 1 Score: 47.0 bits (110), Expect = 8.6e-06
Identity = 29/81 (35.80%), Postives = 43/81 (53.09%), Query Frame = 0


Query: 1  MSKFIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDVCEGGETK 60
          M KF  +F +  L+  +L  A+   P   +T+T+L   +   +   +K   + C G   +
Sbjct: 1  MGKFTTIFIMALLLCSTLTYAARLTP---TTTTALSRENSVKEIEGDKVEEESCNGIGEE 60

Query: 61 ECLARRTLEAHTDYIYTDNTK 82
          ECL RR+L  HTDYIYT N K
Sbjct: 61 ECLIRRSLVLHTDYIYTQNHK 78

[Close]

BLAST of CmaCh12G006060 vs. TAIR 10
Match: AT5G65870.1 (phytosulfokine 5 precursor )

HSP 1 Score: 42.7 bits (99), Expect = 1.6e-04
Identity = 32/81 (39.51%), Postives = 42/81 (51.85%), Query Frame = 0


Query: 1  MSKFIALFSIVALVTMSLFSASEARPIATSTSTSLDFNHIAHQAISEKDHGDV-CEGGET 60
          M KF     I+AL+  S  + + AR   TS        +    +  + + G+V CEG   
Sbjct: 1  MVKFTTFLCIIALLLCSTLTHASARLNPTSV-------YPEENSFKKLEQGEVICEGVGE 60

Query: 61 KEC-LARRTLEAHTDYIYTDN 80
          +EC L RRTL AHTDYIYT N
Sbjct: 61 EECFLIRRTLVAHTDYIYTQN 74

The following BLAST results are available for this feature:

BLAST of CmaCh12G006060 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9FS10	2.2e-06	44.87	Phytosulfokines OS=Asparagus officinalis OX=4686 GN=PSK PE=1 SV=1	[more]
Q9AR88	1.9e-05	49.32	Phytosulfokines 4 OS=Oryza sativa subsp. japonica OX=39947 GN=PSK4 PE=1 SV=1	[more]
Q9M2Y0	1.2e-04	35.80	Phytosulfokines 3 OS=Arabidopsis thaliana OX=3702 GN=PSK3 PE=2 SV=2	[more]

BLAST of CmaCh12G006060 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1BQV2	8.4e-09	51.81	Phytosulfokine-beta OS=Momordica charantia OX=3673 GN=LOC111004777 PE=3 SV=1	[more]
A0A6J1BSJ0	1.3e-06	53.09	Phytosulfokine-beta OS=Momordica charantia OX=3673 GN=LOC111005149 PE=3 SV=1	[more]
A0A2U1NSB2	9.6e-05	45.78	Phytosulfokine-beta OS=Artemisia annua OX=35608 GN=CTI12_AA235340 PE=3 SV=1	[more]
A0A118JW21	9.6e-05	38.89	Phytosulfokine-beta OS=Cynara cardunculus var. scolymus OX=59895 GN=Ccrd_004598 ...	[more]
A0A1U7X3S7	2.1e-04	48.10	Phytosulfokine-beta OS=Nicotiana sylvestris OX=4096 GN=LOC104230093 PE=3 SV=1	[more]

BLAST of CmaCh12G006060 vs. NCBI nr


Match Name	E-value	Identity	Description
KAG6585714.1	4.0e-29	82.93	Phytosulfokine, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023538273.1	8.2e-27	80.49	phytosulfokines-like [Cucurbita pepo subsp. pepo]	[more]
KAG6585713.1	1.2e-12	57.14	Phytosulfokines 3, partial [Cucurbita argyrosperma subsp. sororia]	[more]
XP_023546620.1	1.2e-09	57.32	phytosulfokines-like [Cucurbita pepo subsp. pepo]	[more]
XP_022131649.1	1.7e-08	51.81	uncharacterized protein LOC111004777 [Momordica charantia]	[more]

BLAST of CmaCh12G006060 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G49780.1	8.6e-06	35.80	phytosulfokine 4 precursor	[more]
AT5G65870.1	1.6e-04	39.51	phytosulfokine 5 precursor	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR009438	Phytosulfokine	PFAM	PF06404	PSK	coord: 39..81 e-value: 5.7E-12 score: 46.5
IPR009438	Phytosulfokine	PANTHER	PTHR33285	PHYTOSULFOKINES 3	coord: 1..81
None	No IPR available	PANTHER	PTHR33285:SF1	PHYTOSULFOKINES 3	coord: 1..81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G006060.1	CmaCh12G006060.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0008283	cell population proliferation
cellular_component	GO:0005576	extracellular region
molecular_function	GO:0008083	growth factor activity