CmaCh12G006050 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G006050
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRING-type E3 ubiquitin transferase
LocationCma_Chr12: 3181039 .. 3182391 (+)
RNA-Seq ExpressionCmaCh12G006050
SyntenyCmaCh12G006050
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCGATATACCAGCCGTCTACTTGGAAAGCAGGGGATCCCAAGCTTGTAGTAGCTTGCGGAGCGCAGATTCTAGATCTGGAAACTGTCGTCAAAGATGGGATTCTCGGCGGAGGCGGCGGTGGCGGTGGCGGTGGCGGTGGGGAGAAGTTAGATCTGAAGAAGATGATTGTGGAGCTGGAATCGTCGATAGAGGTCCCATCGGTGTTCATTTGCCCAATTTCTCTCGAGCCGATGCAAGACCCGGTGACACTCTGTTCTGGGCAGACCTACGAGCGGTGCAACATTCAGAAATGGTTCTCTTTAGGCCATTTGACTTGCCCCACAACAATGCAAGAGCTTTGGGACGATTCCCTTACACCCAATCGGACCCTTCACCAGTTGATTCACACCTGGTTTTCGCAGAAGTTCTTAGCGTTCAAGAAGAAATCAGAGGACGTTCAGGGGAGAGTAACAGAGCTTTTGGATTCATTGAAGAAGGTCAAGGGTCAAGCTAGAGTTCAGTCCCTGAAAGATCTCCGACATGTTGTCGCCGCCCATTCCTCCGCCAAAAAGATGGTGGTCGACAACGGCGGCGTCGCTGTAATCTCTTCTCTTTTGGGTTCTTTCACTTCCCACGCAGTTGGGTCGGAAGCCGTGGGGATTCTCGTAAATTTGGATCTCGATTCGGAGTTAAAGAACAATTTGATTCAACCCACAAGAAGATCACTAATTGCAGATATTTTAAACGAAGGCTCCATCGAAACCAAGATCAATTGCACGAAATTCATCAAGAAAATAATGGACGGTGACGATTCAAGAACAGAGAGCGTTTCAAGTTTGAGTTTACTCGTGGGATTGTTGAGAATGGTAAAGGATCAAAAACACCCAAATGGAGTAACCGCCGGACTTAGTTTACTCCGATCGTTCTGTTCAAACGAATCCATAAAAAAGCAACTGGTAAGCATCGGCGCCGTCGCGAAACTCGGCGGTGCTCTGTCCAGTTTCAACGGTGATGGGTTGGAATCAGCGCTTTACATTCTCGACGGTTTGTCGTGTCTTCCAGAGGGGAGATTAGCGTTGAAAGATTGTGAAGAAACGATTAAGATGTTGGTGAAATTATTGATGAAAGTATCTGAGATTTGTACTGAATTTGCGTTGTCAATTTTATGGGCGGTTTTCAAGGTGGCGCCGGAGGAATGCGCGGTGGTTGCGGTGGATGCAGGATTGGCGGCCAAGTTGCTGCTAGTGATACAAAGTGGGTGCAATAAAGAGGTAAAACAGAGATCATCAGAGCTATTGAAATTGTGTAGTCTAAATTACCCGGATTCCATCTTCATTTCAAAGTGTAAGCTTACAACAACTATACAATAA

mRNA sequence

ATGCCGATATACCAGCCGTCTACTTGGAAAGCAGGGGATCCCAAGCTTGTAGTAGCTTGCGGAGCGCAGATTCTAGATCTGGAAACTGTCGTCAAAGATGGGATTCTCGGCGGAGGCGGCGGTGGCGGTGGCGGTGGCGGTGGGGAGAAGTTAGATCTGAAGAAGATGATTGTGGAGCTGGAATCGTCGATAGAGGTCCCATCGGTGTTCATTTGCCCAATTTCTCTCGAGCCGATGCAAGACCCGGTGACACTCTGTTCTGGGCAGACCTACGAGCGGTGCAACATTCAGAAATGGTTCTCTTTAGGCCATTTGACTTGCCCCACAACAATGCAAGAGCTTTGGGACGATTCCCTTACACCCAATCGGACCCTTCACCAGTTGATTCACACCTGGTTTTCGCAGAAGTTCTTAGCGTTCAAGAAGAAATCAGAGGACGTTCAGGGGAGAGTAACAGAGCTTTTGGATTCATTGAAGAAGGTCAAGGGTCAAGCTAGAGTTCAGTCCCTGAAAGATCTCCGACATGTTGTCGCCGCCCATTCCTCCGCCAAAAAGATGGTGGTCGACAACGGCGGCGTCGCTGTAATCTCTTCTCTTTTGGGTTCTTTCACTTCCCACGCAGTTGGGTCGGAAGCCGTGGGGATTCTCGTAAATTTGGATCTCGATTCGGAGTTAAAGAACAATTTGATTCAACCCACAAGAAGATCACTAATTGCAGATATTTTAAACGAAGGCTCCATCGAAACCAAGATCAATTGCACGAAATTCATCAAGAAAATAATGGACGGTGACGATTCAAGAACAGAGAGCGTTTCAAGTTTGAGTTTACTCGTGGGATTGTTGAGAATGGTAAAGGATCAAAAACACCCAAATGGAGTAACCGCCGGACTTAGTTTACTCCGATCGTTCTGTTCAAACGAATCCATAAAAAAGCAACTGGTAAGCATCGGCGCCGTCGCGAAACTCGGCGGTGCTCTGTCCAGTTTCAACGGTGATGGGTTGGAATCAGCGCTTTACATTCTCGACGGTTTGTCGTGTCTTCCAGAGGGGAGATTAGCGTTGAAAGATTGTGAAGAAACGATTAAGATGTTGGTGAAATTATTGATGAAAGTATCTGAGATTTGTACTGAATTTGCGTTGTCAATTTTATGGGCGGTTTTCAAGGTGGCGCCGGAGGAATGCGCGGTGGTTGCGGTGGATGCAGGATTGGCGGCCAAGTTGCTGCTAGTGATACAAAGTGGGTGCAATAAAGAGGTAAAACAGAGATCATCAGAGCTATTGAAATTGTGTAGTCTAAATTACCCGGATTCCATCTTCATTTCAAAGTGTAAGCTTACAACAACTATACAATAA

Coding sequence (CDS)

ATGCCGATATACCAGCCGTCTACTTGGAAAGCAGGGGATCCCAAGCTTGTAGTAGCTTGCGGAGCGCAGATTCTAGATCTGGAAACTGTCGTCAAAGATGGGATTCTCGGCGGAGGCGGCGGTGGCGGTGGCGGTGGCGGTGGGGAGAAGTTAGATCTGAAGAAGATGATTGTGGAGCTGGAATCGTCGATAGAGGTCCCATCGGTGTTCATTTGCCCAATTTCTCTCGAGCCGATGCAAGACCCGGTGACACTCTGTTCTGGGCAGACCTACGAGCGGTGCAACATTCAGAAATGGTTCTCTTTAGGCCATTTGACTTGCCCCACAACAATGCAAGAGCTTTGGGACGATTCCCTTACACCCAATCGGACCCTTCACCAGTTGATTCACACCTGGTTTTCGCAGAAGTTCTTAGCGTTCAAGAAGAAATCAGAGGACGTTCAGGGGAGAGTAACAGAGCTTTTGGATTCATTGAAGAAGGTCAAGGGTCAAGCTAGAGTTCAGTCCCTGAAAGATCTCCGACATGTTGTCGCCGCCCATTCCTCCGCCAAAAAGATGGTGGTCGACAACGGCGGCGTCGCTGTAATCTCTTCTCTTTTGGGTTCTTTCACTTCCCACGCAGTTGGGTCGGAAGCCGTGGGGATTCTCGTAAATTTGGATCTCGATTCGGAGTTAAAGAACAATTTGATTCAACCCACAAGAAGATCACTAATTGCAGATATTTTAAACGAAGGCTCCATCGAAACCAAGATCAATTGCACGAAATTCATCAAGAAAATAATGGACGGTGACGATTCAAGAACAGAGAGCGTTTCAAGTTTGAGTTTACTCGTGGGATTGTTGAGAATGGTAAAGGATCAAAAACACCCAAATGGAGTAACCGCCGGACTTAGTTTACTCCGATCGTTCTGTTCAAACGAATCCATAAAAAAGCAACTGGTAAGCATCGGCGCCGTCGCGAAACTCGGCGGTGCTCTGTCCAGTTTCAACGGTGATGGGTTGGAATCAGCGCTTTACATTCTCGACGGTTTGTCGTGTCTTCCAGAGGGGAGATTAGCGTTGAAAGATTGTGAAGAAACGATTAAGATGTTGGTGAAATTATTGATGAAAGTATCTGAGATTTGTACTGAATTTGCGTTGTCAATTTTATGGGCGGTTTTCAAGGTGGCGCCGGAGGAATGCGCGGTGGTTGCGGTGGATGCAGGATTGGCGGCCAAGTTGCTGCTAGTGATACAAAGTGGGTGCAATAAAGAGGTAAAACAGAGATCATCAGAGCTATTGAAATTGTGTAGTCTAAATTACCCGGATTCCATCTTCATTTCAAAGTGTAAGCTTACAACAACTATACAATAA

Protein sequence

MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLTPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAHSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIADILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLLRSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEETIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVKQRSSELLKLCSLNYPDSIFISKCKLTTTIQ
Homology
BLAST of CmaCh12G006050 vs. ExPASy Swiss-Prot
Match: Q058P4 (U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1 SV=1)

HSP 1 Score: 526.9 bits (1356), Expect = 2.2e-148
Identity = 277/450 (61.56%), Postives = 349/450 (77.56%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL 60
           MP++QP   + G          Q+LDL+T VKDG+LGG  GGG G   EKLDLKKMI EL
Sbjct: 1   MPMFQPLK-RDGLIGFEGGGDGQVLDLDTAVKDGVLGGVNGGGVGVVDEKLDLKKMIKEL 60

Query: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120
           +   ++PSVFICPISLEPMQDPVTLC+GQTYER NI KWF+LGHLTCPTTMQELWDD++T
Sbjct: 61  DLQ-DIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQELWDDTVT 120

Query: 121 PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180
           PN+TLH LI+TWFSQK++  KK+SEDVQGR  E+L +LKK KGQARV +L +L+ +V AH
Sbjct: 121 PNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELKQIVIAH 180

Query: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD 240
             A+K VV+ GGV+VISSLLG FTSHAVGSE V ILV+LDLDS+ K+ L+QP + SLI D
Sbjct: 181 LMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQPAKVSLIVD 240

Query: 241 ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300
           +LN+GS ETKINC + I+ +++    R E VSS SLLVGL+R+VKD++H NGV+  L LL
Sbjct: 241 MLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNGVSPALRLL 300

Query: 301 RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET 360
           +    ++ ++  +VSIGAV +L   L S + + LE AL++LD L    EGR+A+KD   T
Sbjct: 301 KPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRVAVKDSANT 360

Query: 361 IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420
           I   V++LM+VSE CT +ALSILW+V K+APEEC+ +AV+ GLAAKLLLVIQSGC+  +K
Sbjct: 361 IPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQSGCDAALK 420

Query: 421 QRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           QRS+ELLKLCSL+Y D++FISKCKLT TIQ
Sbjct: 421 QRSAELLKLCSLHYSDTMFISKCKLTRTIQ 448

BLAST of CmaCh12G006050 vs. ExPASy Swiss-Prot
Match: Q9FHN9 (U-box domain-containing protein 31 OS=Arabidopsis thaliana OX=3702 GN=PUB31 PE=2 SV=1)

HSP 1 Score: 511.1 bits (1315), Expect = 1.2e-143
Identity = 276/453 (60.93%), Postives = 343/453 (75.72%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGG---GGGGGGEKLDLKKMI 60
           MP++QPS     D          ILDL + VKDG+LGGG G          +KLDLK+MI
Sbjct: 1   MPMFQPSKNGGFD--------GHILDLHSAVKDGVLGGGDGKFLVVVTDEKKKLDLKEMI 60

Query: 61  VELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDD 120
            ELE   E+PSVFICPISLEPMQDPVTLC+GQTYER NI KWF++GH TCPTTMQELWDD
Sbjct: 61  SELELP-EIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQELWDD 120

Query: 121 SLTPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVV 180
            +TPN+TLHQLI+TWFSQK++  KK+SEDVQGR  E+L +L+K KG+A+V +L +L+ VV
Sbjct: 121 LVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSELKQVV 180

Query: 181 AAHSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSL 240
            AH+ AKK VVD GGV VISSLL  FTSHAVGSEA+ ILVNL+LDS+ K  L+QP R SL
Sbjct: 181 MAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQPARVSL 240

Query: 241 IADILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGL 300
           + D+LN+GSIETKINC + I ++++    R E VSS SLLVGL+R+VKD++  NGV+  L
Sbjct: 241 MVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNGVSPAL 300

Query: 301 SLLRSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDC 360
           +LL+S   ++ ++  LV IGAV +L   L   + + LESAL++LD L    EGR+ALKD 
Sbjct: 301 TLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRIALKDS 360

Query: 361 EETIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNK 420
             TI   V+LLMKVSE CT +A+SILW+V K+A EEC+ +AV+ GLAAKLLLVIQSGC+ 
Sbjct: 361 VNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQSGCDP 420

Query: 421 EVKQRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
            +KQRS+ELLKLCSL+Y DS+FISKCKLT TIQ
Sbjct: 421 ALKQRSAELLKLCSLHYSDSMFISKCKLTRTIQ 444

BLAST of CmaCh12G006050 vs. ExPASy Swiss-Prot
Match: Q10PI9 (U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=TUD1 PE=1 SV=1)

HSP 1 Score: 464.9 bits (1195), Expect = 1.0e-129
Identity = 246/451 (54.55%), Postives = 330/451 (73.17%), Query Frame = 0

Query: 20  CGAQILDLETVVKDGILGG----GGGGGGGGGGEKLDLKKMIVELESSIE------VPSV 79
           CG Q+LD++T +KDGILGG    G    G GG + ++L+KM+ EL+++ +      VP+V
Sbjct: 9   CGGQVLDIDTALKDGILGGGPELGDAAAGDGGKQPVELRKMMDELDAAGDGGGDEAVPAV 68

Query: 80  FICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLTPNRTLHQLI 139
           FICPISLEPM DPVTLC+GQTYE  NI +W +LGH TCPTTMQELWD +  PN TL QLI
Sbjct: 69  FICPISLEPMVDPVTLCTGQTYESANISRWLALGHRTCPTTMQELWDVTPIPNTTLRQLI 128

Query: 140 HTWFSQKFLAFKKKSEDVQGRVTELLDSLK--------KVKGQARVQSLKDLRHVVAAHS 199
             WFS+++  FKK+S D  GR  EL+ +L+         +KGQARV +L++LR + AAH 
Sbjct: 129 AAWFSRRYTRFKKRSADFHGRAAELVHALRGTAVPKRQPLKGQARVAALRELRSLAAAHQ 188

Query: 200 SAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVN-LDLDSELKNNLIQPTRRSLIAD 259
           S  K + + GGV +++SLLG FTSHAVGSEAV ILV+ + LD++ K  L+QP + SL+ D
Sbjct: 189 SVTKAIAEAGGVGLLTSLLGPFTSHAVGSEAVAILVSGVPLDADAKAALMQPAKVSLLVD 248

Query: 260 ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 319
           +LNEG+++TKINC + I+ +M+    R ++V+SLSLLVG++R+V+D++HP+GV AGL LL
Sbjct: 249 MLNEGAVDTKINCVRLIRILMEEKGFRPDTVASLSLLVGVMRLVRDKRHPDGVAAGLELL 308

Query: 320 RSFCS-NESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEE 379
            S C+ ++  +  +VSIGAV +L   L     + +E AL ILD L+ +PEGR+ALKDC  
Sbjct: 309 NSICAVHKPARSLIVSIGAVPQLVELLPELPTECVEPALDILDALAAVPEGRIALKDCPR 368

Query: 380 TIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEV 439
           TI   V+LLM+VSE CT  ALS+LW V ++APEECA  A+DAGL AKLLLVIQSGC  E+
Sbjct: 369 TITNAVRLLMRVSEACTRRALSMLWVVCRMAPEECAPAALDAGLGAKLLLVIQSGCGPEL 428

Query: 440 KQRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           KQ++SELLKLC++N   ++FISKCKLT TIQ
Sbjct: 429 KQQASELLKLCTMNCTSTVFISKCKLTKTIQ 459

BLAST of CmaCh12G006050 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 154.1 bits (388), Expect = 3.8e-36
Identity = 127/406 (31.28%), Postives = 206/406 (50.74%), Query Frame = 0

Query: 53  LKKMIVELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLG-HLTCPTTM 112
           + + I  L+  I++P  F CPISLE MQDPVT+C+GQTY+R +I+ W S+G + TCP T 
Sbjct: 1   MPRNIEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTR 60

Query: 113 QELWDDSLTPNRTLHQLIHTWF------SQKFLAFKKKSEDVQGRVTELLDSLKKVKG-- 172
             L D +L PN TL +LI  W         + +   K+  D    V  LL     + G  
Sbjct: 61  APLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTS-VRALLSQASAITGTH 120

Query: 173 ---QARVQSLKDLRHVVAAHSSAKKMVV---DNGGVAVISSLLGSFTSHAVGSEAVGILV 232
              ++R  +L+ LR    A  S K  V+    N    +I  L    TS  + SE++ +LV
Sbjct: 121 VSVRSRAAALRRLRGF--ARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLV 180

Query: 233 NLDL--DSELKNNLIQPTRRSLIADILNEGSIETKINCTKFIKKIMDGD---DSRTESVS 292
            L +   ++  +    P R   +  +L + SIET++N    I+ +  G    D +    +
Sbjct: 181 MLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISN 240

Query: 293 SLSLLVGLLRMVKDQ-KHPNGVTAGLSLLRSFCSNESIKKQLVSIGAVAKLGGALSS-FN 352
           S S+  G+L ++++       +  G+  L + CS +S +   ++ GA   L   L++ F+
Sbjct: 241 SESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFD 300

Query: 353 GDGLESALYILDGLSCLPEGRLALKDCEETIKMLVKLLMKVSEICTEFALSILWAVFKVA 412
               E AL  ++ L   PEG  A  +   T+ +LVK +++VS+  TE+A   L A+   A
Sbjct: 301 RCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC-TA 360

Query: 413 PEECAVVAVDAGLAAKLLLVIQSGCNKEVKQRSSELLKLCSLNYPD 437
            E     A  AG+  +LLL++QS C +  K+++ +LLKL   ++PD
Sbjct: 361 EERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPD 402

BLAST of CmaCh12G006050 vs. ExPASy Swiss-Prot
Match: Q9FXA4 (U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2 SV=1)

HSP 1 Score: 146.7 bits (369), Expect = 6.1e-34
Identity = 115/401 (28.68%), Postives = 202/401 (50.37%), Query Frame = 0

Query: 60  LESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSL 119
           L+  I++P  F CPISL+ M DPVT+ +GQTY+R +I  W ++G+ TCP T   L D +L
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTL 67

Query: 120 TPNRTLHQLIHTWF-------SQKFLAFKKKSEDVQGRVTELLDSLKKVKG-----QARV 179
            PN TL +LI  W         ++    K+ ++ +   V  LL     + G     ++R 
Sbjct: 68  IPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPIS--VRSLLSQASAITGTHVSVRSRA 127

Query: 180 QSLKDLRHVVAAHSSAKKMVVDNGGVAVISSLL------GSFTSHAVGSEAVGILVNLDL 239
            +++ LR +       + ++  +    ++  +L       S +S  V SE++ +LV L +
Sbjct: 128 AAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV-SESLALLVLLHM 187

Query: 240 -DSELKNNLIQPTRRSLIADILNEGSIETKINCTKFIKKIMDGDDSRTESV---SSLSLL 299
            ++E +     P+R   +  +L + SIE ++N    I+ ++ G  S    +    S S+ 
Sbjct: 188 TETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIF 247

Query: 300 VGLLRMVKDQ-KHPNGVTAGLSLLRSFCSNESIKKQLVSIGAVAKLGGALSS-FNGDGLE 359
            G+L ++K+       +  G+  + + C  +  +   +S GA   L   L++ F+    E
Sbjct: 248 EGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTE 307

Query: 360 SALYILDGLSCLPEGRLALKDCEETIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECA 419
             L  ++ L  LPEG  A  +   T+ ++VK +++VS+  TE+A   L A+   A E C 
Sbjct: 308 RGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC-TAEERCR 367

Query: 420 VVAVDAGLAAKLLLVIQSGCNKEVKQRSSELLKLCSLNYPD 437
             A  AGL  +LLL++QS C +  K+++  LLKL   ++PD
Sbjct: 368 DEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPD 404

BLAST of CmaCh12G006050 vs. ExPASy TrEMBL
Match: A0A6J1KKN5 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111496578 PE=4 SV=1)

HSP 1 Score: 872.8 bits (2254), Expect = 6.0e-250
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL 60
           MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL
Sbjct: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL 60

Query: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120
           ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT
Sbjct: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120

Query: 121 PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180
           PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH
Sbjct: 121 PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180

Query: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD 240
           SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD
Sbjct: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD 240

Query: 241 ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300
           ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL
Sbjct: 241 ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300

Query: 301 RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET 360
           RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET
Sbjct: 301 RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET 360

Query: 361 IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420
           IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK
Sbjct: 361 IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420

Query: 421 QRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           QRSSELLKLCSLNYPDSIFISKCKLTTTIQ
Sbjct: 421 QRSSELLKLCSLNYPDSIFISKCKLTTTIQ 450

BLAST of CmaCh12G006050 vs. ExPASy TrEMBL
Match: A0A6J1GHN9 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111454038 PE=4 SV=1)

HSP 1 Score: 837.8 bits (2163), Expect = 2.1e-239
Identity = 435/450 (96.67%), Postives = 441/450 (98.00%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL 60
           MP+YQPSTW+AG+PKLVVACGAQILDLETVVKDGIL GGGGGGGGGGGEKLDLK+MIVEL
Sbjct: 1   MPMYQPSTWRAGNPKLVVACGAQILDLETVVKDGIL-GGGGGGGGGGGEKLDLKEMIVEL 60

Query: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120
           ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT
Sbjct: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120

Query: 121 PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180
           PNRTLHQLIHTWFSQKF AFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH
Sbjct: 121 PNRTLHQLIHTWFSQKFFAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180

Query: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD 240
           SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTR SLIAD
Sbjct: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRISLIAD 240

Query: 241 ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300
           ILNEGSIETKINCTKFIKK+MDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL
Sbjct: 241 ILNEGSIETKINCTKFIKKLMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300

Query: 301 RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET 360
           RS CSNESIKKQLVSIGAV KLGGALSSF GDGLESALYILDGLS LPEGRLALKDCEET
Sbjct: 301 RSLCSNESIKKQLVSIGAVGKLGGALSSFIGDGLESALYILDGLSSLPEGRLALKDCEET 360

Query: 361 IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420
           IK LVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK
Sbjct: 361 IKNLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420

Query: 421 QRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           QRSSELLKLC+LNYPDSIFISKCKLT TIQ
Sbjct: 421 QRSSELLKLCTLNYPDSIFISKCKLTRTIQ 449

BLAST of CmaCh12G006050 vs. ExPASy TrEMBL
Match: A0A0A0LKY4 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G354100 PE=4 SV=1)

HSP 1 Score: 761.1 bits (1964), Expect = 2.5e-216
Identity = 394/455 (86.59%), Postives = 422/455 (92.75%), Query Frame = 0

Query: 1   MPIYQP-STWKAGDPKLVVACGAQILDLETVVKDGILGGGG----GGGGGGGGEKLDLKK 60
           MP+YQP +TW+ GDPKLVV CG QILDLETVVKDGILGGGG    GGGGGGGGEKLDLK+
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGGGGGEKLDLKE 60

Query: 61  MIVELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELW 120
           MI ELESS+EVPSVFICPISLEPMQDPVTLCSGQTYER NI KWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSVEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSLTPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRH 180
           DDS+TPNRTLHQLIHTWFSQK+LA KK+SEDVQGRV ELLDSLKKVKGQARVQSLKDLRH
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRH 180

Query: 181 VVAAHSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRR 240
           +V  HSSAKKMVVDNGGVA+ISSLLGSFTSHAVGSEAVGILVNLDL+SELK NLIQPTR 
Sbjct: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTA 300
           SLIADILNEGSIETKINCTKF K ++ G+D +TE VSSLSLLVGLLRMVKDQ+HPNGVTA
Sbjct: 241 SLIADILNEGSIETKINCTKFFKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALK 360
           GLSLLR+ CSNE I+KQLVSIGAVAKLGG LS+ N D LESALYILD LS LPEGRLALK
Sbjct: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNTDCLESALYILDVLSSLPEGRLALK 360

Query: 361 DCEETIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGC 420
           +CE+TI+ LVKLLMKVSEICT+FALSILWAVFK+APEECAV+AV+AGLAAKLLLVIQSGC
Sbjct: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGC 420

Query: 421 NKEVKQRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           N E+KQRS+ELLKLCSLNYPDSIFISKCKLT TIQ
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

BLAST of CmaCh12G006050 vs. ExPASy TrEMBL
Match: A0A5A7UYA8 (RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G00980 PE=4 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.4e-216
Identity = 395/455 (86.81%), Postives = 422/455 (92.75%), Query Frame = 0

Query: 1   MPIYQP-STWKAGDPKLVVACGAQILDLETVVKDGILGGGG----GGGGGGGGEKLDLKK 60
           MP+YQP +TW+ GDPKLVV CG QILDLETVVKDGILGGGG    GGGGGGGGEKLDLK+
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGGGGGEKLDLKE 60

Query: 61  MIVELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELW 120
           MI ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYER NI KWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSLTPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRH 180
           DDS+TPNRTLHQLIHTWFSQK+LA KK+SEDVQGRV ELLDSLKKVKGQARVQSLKDLR 
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRL 180

Query: 181 VVAAHSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRR 240
           +V  HSSAKKMVVDNGGVA+ISSLLGSFTSHAVGSEAVGILVNLDL+SELK NLIQPTR 
Sbjct: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTA 300
           SLIADILNEGSIETKINCTKFIK ++ G+D +TE VSSLSLLVGLLRMVKDQ+HPNGVTA
Sbjct: 241 SLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALK 360
           GLSLLR+ CSNE I+KQLVSIGAVAKLGG LS+ N D LESALYILD LS LPEGRLALK
Sbjct: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLALK 360

Query: 361 DCEETIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGC 420
           +CE+TI+ LVKLLMKVSEICT+FALSILWAVFK+APEECAV+AV+AGLAAKLLLVIQSGC
Sbjct: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGC 420

Query: 421 NKEVKQRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           N E+KQRS+ELLKLCSLNYPDSIFISKCKLT TIQ
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

BLAST of CmaCh12G006050 vs. ExPASy TrEMBL
Match: A0A1S3BA20 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487822 PE=4 SV=1)

HSP 1 Score: 759.6 bits (1960), Expect = 7.4e-216
Identity = 395/455 (86.81%), Postives = 422/455 (92.75%), Query Frame = 0

Query: 1   MPIYQP-STWKAGDPKLVVACGAQILDLETVVKDGILGGGG----GGGGGGGGEKLDLKK 60
           MP+YQP +TW+ GDPKLVV CG QILDLETVVKDGILGGGG    GGGGGGGGEKLDLK+
Sbjct: 1   MPMYQPATTWREGDPKLVVGCGTQILDLETVVKDGILGGGGGIICGGGGGGGGEKLDLKE 60

Query: 61  MIVELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELW 120
           MI ELESSIEVPSVFICPISLEPMQDPVTLCSGQTYER NI KWFSLGHLTCPTTMQELW
Sbjct: 61  MIEELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERSNILKWFSLGHLTCPTTMQELW 120

Query: 121 DDSLTPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRH 180
           DDS+TPNRTLHQLIHTWFSQK+LA KK+SEDVQGRV ELLDSLKKVKGQARVQSLKDLR 
Sbjct: 121 DDSVTPNRTLHQLIHTWFSQKYLALKKRSEDVQGRVLELLDSLKKVKGQARVQSLKDLRL 180

Query: 181 VVAAHSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRR 240
           +V  HSSAKKMVVDNGGVA+ISSLLGSFTSHAVGSEAVGILVNLDL+SELK NLIQPTR 
Sbjct: 181 IVITHSSAKKMVVDNGGVALISSLLGSFTSHAVGSEAVGILVNLDLNSELKKNLIQPTRI 240

Query: 241 SLIADILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTA 300
           SLIADILNEGSIETKINCTKFIK ++ G+D +TE VSSLSLLVGLLRMVKDQ+HPNGVTA
Sbjct: 241 SLIADILNEGSIETKINCTKFIKMLIVGEDLKTEDVSSLSLLVGLLRMVKDQRHPNGVTA 300

Query: 301 GLSLLRSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALK 360
           GLSLLR+ CSNE I+KQLVSIGAVAKLGG LS+ N D LESALYILD LS LPEGRLALK
Sbjct: 301 GLSLLRTLCSNEPIRKQLVSIGAVAKLGGTLSNLNADCLESALYILDVLSSLPEGRLALK 360

Query: 361 DCEETIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGC 420
           +CE+TI+ LVKLLMKVSEICT+FALSILWAVFK+APEECAV+AV+AGLAAKLLLVIQSGC
Sbjct: 361 NCEDTIRNLVKLLMKVSEICTQFALSILWAVFKLAPEECAVLAVEAGLAAKLLLVIQSGC 420

Query: 421 NKEVKQRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           N E+KQRS+ELLKLCSLNYPDSIFISKCKLT TIQ
Sbjct: 421 NPELKQRSAELLKLCSLNYPDSIFISKCKLTRTIQ 455

BLAST of CmaCh12G006050 vs. NCBI nr
Match: XP_023002832.1 (U-box domain-containing protein 30-like [Cucurbita maxima])

HSP 1 Score: 872.8 bits (2254), Expect = 1.2e-249
Identity = 450/450 (100.00%), Postives = 450/450 (100.00%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL 60
           MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL
Sbjct: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL 60

Query: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120
           ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT
Sbjct: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120

Query: 121 PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180
           PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH
Sbjct: 121 PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180

Query: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD 240
           SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD
Sbjct: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD 240

Query: 241 ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300
           ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL
Sbjct: 241 ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300

Query: 301 RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET 360
           RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET
Sbjct: 301 RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET 360

Query: 361 IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420
           IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK
Sbjct: 361 IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420

Query: 421 QRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           QRSSELLKLCSLNYPDSIFISKCKLTTTIQ
Sbjct: 421 QRSSELLKLCSLNYPDSIFISKCKLTTTIQ 450

BLAST of CmaCh12G006050 vs. NCBI nr
Match: XP_022951085.1 (U-box domain-containing protein 30-like [Cucurbita moschata])

HSP 1 Score: 837.8 bits (2163), Expect = 4.4e-239
Identity = 435/450 (96.67%), Postives = 441/450 (98.00%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL 60
           MP+YQPSTW+AG+PKLVVACGAQILDLETVVKDGIL GGGGGGGGGGGEKLDLK+MIVEL
Sbjct: 1   MPMYQPSTWRAGNPKLVVACGAQILDLETVVKDGIL-GGGGGGGGGGGEKLDLKEMIVEL 60

Query: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120
           ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT
Sbjct: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120

Query: 121 PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180
           PNRTLHQLIHTWFSQKF AFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH
Sbjct: 121 PNRTLHQLIHTWFSQKFFAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180

Query: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD 240
           SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTR SLIAD
Sbjct: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRISLIAD 240

Query: 241 ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300
           ILNEGSIETKINCTKFIKK+MDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL
Sbjct: 241 ILNEGSIETKINCTKFIKKLMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300

Query: 301 RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET 360
           RS CSNESIKKQLVSIGAV KLGGALSSF GDGLESALYILDGLS LPEGRLALKDCEET
Sbjct: 301 RSLCSNESIKKQLVSIGAVGKLGGALSSFIGDGLESALYILDGLSSLPEGRLALKDCEET 360

Query: 361 IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420
           IK LVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK
Sbjct: 361 IKNLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420

Query: 421 QRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           QRSSELLKLC+LNYPDSIFISKCKLT TIQ
Sbjct: 421 QRSSELLKLCTLNYPDSIFISKCKLTRTIQ 449

BLAST of CmaCh12G006050 vs. NCBI nr
Match: XP_023536979.1 (U-box domain-containing protein 30-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 834.3 bits (2154), Expect = 4.9e-238
Identity = 434/451 (96.23%), Postives = 440/451 (97.56%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGG-GGGGGGGEKLDLKKMIVE 60
           MP+YQPSTW+ GDPKLVVACGAQILDLETVVKDGILGGGGG GGGGGGGEKLDLK+MI  
Sbjct: 1   MPMYQPSTWREGDPKLVVACGAQILDLETVVKDGILGGGGGVGGGGGGGEKLDLKEMIEV 60

Query: 61  LESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSL 120
           LESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSL
Sbjct: 61  LESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSL 120

Query: 121 TPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAA 180
           TPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAA
Sbjct: 121 TPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAA 180

Query: 181 HSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIA 240
           HSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTR SLIA
Sbjct: 181 HSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRISLIA 240

Query: 241 DILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSL 300
           DILNEGSIETKINCTKFIKK+ DGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSL
Sbjct: 241 DILNEGSIETKINCTKFIKKLTDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSL 300

Query: 301 LRSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEE 360
           LRS CSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYIL+ LS LPEGRLALKDCEE
Sbjct: 301 LRSLCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILEVLSSLPEGRLALKDCEE 360

Query: 361 TIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEV 420
           TIK LVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEV
Sbjct: 361 TIKKLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEV 420

Query: 421 KQRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           KQRSSELLKLC+LNYPDSIFISKCKLT TIQ
Sbjct: 421 KQRSSELLKLCTLNYPDSIFISKCKLTRTIQ 451

BLAST of CmaCh12G006050 vs. NCBI nr
Match: KAG6585712.1 (U-box domain-containing protein 31, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 824.3 bits (2128), Expect = 5.0e-235
Identity = 429/448 (95.76%), Postives = 435/448 (97.10%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGIL------GGGGGGGGGGGGEKLDLK 60
           MP+YQPSTW+AG+PKLVVACGAQILDLETVVKDGIL      GGGGGGGGGGGGEKLDLK
Sbjct: 1   MPMYQPSTWRAGNPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGGGGGGGEKLDLK 60

Query: 61  KMIVELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQEL 120
           +MIVELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQEL
Sbjct: 61  EMIVELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQEL 120

Query: 121 WDDSLTPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLR 180
           WDDSLTPNRTLHQLIHTWFSQKF AFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLR
Sbjct: 121 WDDSLTPNRTLHQLIHTWFSQKFFAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLR 180

Query: 181 HVVAAHSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTR 240
           HVVAAHSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTR
Sbjct: 181 HVVAAHSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTR 240

Query: 241 RSLIADILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVT 300
            SLIADILNEGSIETKINCTKFIKK+MDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVT
Sbjct: 241 ISLIADILNEGSIETKINCTKFIKKLMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVT 300

Query: 301 AGLSLLRSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLAL 360
           AGLSLLRS CSNESIKKQLVSIGAV KLGGALSSF GDGLESALYILDGLS LPEGRLAL
Sbjct: 301 AGLSLLRSLCSNESIKKQLVSIGAVGKLGGALSSFIGDGLESALYILDGLSSLPEGRLAL 360

Query: 361 KDCEETIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSG 420
           KDCEETIK LVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSG
Sbjct: 361 KDCEETIKNLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSG 420

Query: 421 CNKEVKQRSSELLKLCSLNYPDSIFISK 443
           CNKEVKQRSSELLKLC+LNYPDSIFISK
Sbjct: 421 CNKEVKQRSSELLKLCTLNYPDSIFISK 448

BLAST of CmaCh12G006050 vs. NCBI nr
Match: KAG7020615.1 (U-box domain-containing protein 31, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 780.4 bits (2014), Expect = 8.3e-222
Identity = 407/429 (94.87%), Postives = 413/429 (96.27%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL 60
           MP+YQPSTW+AG+PKLVVACGAQILDLETVVKDGIL GGGGGGGGGGGEKLDLK+MIVEL
Sbjct: 1   MPMYQPSTWRAGNPKLVVACGAQILDLETVVKDGIL-GGGGGGGGGGGEKLDLKEMIVEL 60

Query: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120
           ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT
Sbjct: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120

Query: 121 PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180
           PNRTLHQLIHTWFSQKF AFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH
Sbjct: 121 PNRTLHQLIHTWFSQKFFAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180

Query: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD 240
           SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTR SLIAD
Sbjct: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRISLIAD 240

Query: 241 ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300
           ILNEGSIETKINCTKFIKK+MDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL
Sbjct: 241 ILNEGSIETKINCTKFIKKLMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300

Query: 301 RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET 360
           RS CSNESIKKQLVSIGAV KLGGALSSF GDGLESALYILDGLS LPEGRLALKDCEET
Sbjct: 301 RSLCSNESIKKQLVSIGAVGKLGGALSSFIGDGLESALYILDGLSSLPEGRLALKDCEET 360

Query: 361 IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420
           IK LVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKE  
Sbjct: 361 IKNLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEAH 420

Query: 421 QRSSELLKL 430
              S L  +
Sbjct: 421 LCLSSLANI 428

BLAST of CmaCh12G006050 vs. TAIR 10
Match: AT3G49810.1 (ARM repeat superfamily protein )

HSP 1 Score: 526.9 bits (1356), Expect = 1.5e-149
Identity = 277/450 (61.56%), Postives = 349/450 (77.56%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGGGGGGGGEKLDLKKMIVEL 60
           MP++QP   + G          Q+LDL+T VKDG+LGG  GGG G   EKLDLKKMI EL
Sbjct: 1   MPMFQPLK-RDGLIGFEGGGDGQVLDLDTAVKDGVLGGVNGGGVGVVDEKLDLKKMIKEL 60

Query: 61  ESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLT 120
           +   ++PSVFICPISLEPMQDPVTLC+GQTYER NI KWF+LGHLTCPTTMQELWDD++T
Sbjct: 61  DLQ-DIPSVFICPISLEPMQDPVTLCTGQTYERLNIHKWFNLGHLTCPTTMQELWDDTVT 120

Query: 121 PNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAH 180
           PN+TLH LI+TWFSQK++  KK+SEDVQGR  E+L +LKK KGQARV +L +L+ +V AH
Sbjct: 121 PNKTLHHLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLKKAKGQARVHALSELKQIVIAH 180

Query: 181 SSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSLIAD 240
             A+K VV+ GGV+VISSLLG FTSHAVGSE V ILV+LDLDS+ K+ L+QP + SLI D
Sbjct: 181 LMARKTVVEEGGVSVISSLLGPFTSHAVGSEVVAILVSLDLDSDSKSGLMQPAKVSLIVD 240

Query: 241 ILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGLSLL 300
           +LN+GS ETKINC + I+ +++    R E VSS SLLVGL+R+VKD++H NGV+  L LL
Sbjct: 241 MLNDGSNETKINCARLIRGLVEEKGFRAELVSSHSLLVGLMRLVKDKRHRNGVSPALRLL 300

Query: 301 RSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDCEET 360
           +    ++ ++  +VSIGAV +L   L S + + LE AL++LD L    EGR+A+KD   T
Sbjct: 301 KPISVHKQVRSLMVSIGAVPQLVDILPSLDPECLELALFVLDALCTDVEGRVAVKDSANT 360

Query: 361 IKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNKEVK 420
           I   V++LM+VSE CT +ALSILW+V K+APEEC+ +AV+ GLAAKLLLVIQSGC+  +K
Sbjct: 361 IPYTVRVLMRVSENCTNYALSILWSVCKLAPEECSPLAVEVGLAAKLLLVIQSGCDAALK 420

Query: 421 QRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
           QRS+ELLKLCSL+Y D++FISKCKLT TIQ
Sbjct: 421 QRSAELLKLCSLHYSDTMFISKCKLTRTIQ 448

BLAST of CmaCh12G006050 vs. TAIR 10
Match: AT5G65920.1 (ARM repeat superfamily protein )

HSP 1 Score: 511.1 bits (1315), Expect = 8.8e-145
Identity = 276/453 (60.93%), Postives = 343/453 (75.72%), Query Frame = 0

Query: 1   MPIYQPSTWKAGDPKLVVACGAQILDLETVVKDGILGGGGGG---GGGGGGEKLDLKKMI 60
           MP++QPS     D          ILDL + VKDG+LGGG G          +KLDLK+MI
Sbjct: 1   MPMFQPSKNGGFD--------GHILDLHSAVKDGVLGGGDGKFLVVVTDEKKKLDLKEMI 60

Query: 61  VELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDD 120
            ELE   E+PSVFICPISLEPMQDPVTLC+GQTYER NI KWF++GH TCPTTMQELWDD
Sbjct: 61  SELELP-EIPSVFICPISLEPMQDPVTLCTGQTYERSNILKWFNIGHCTCPTTMQELWDD 120

Query: 121 SLTPNRTLHQLIHTWFSQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVV 180
            +TPN+TLHQLI+TWFSQK++  KK+SEDVQGR  E+L +L+K KG+A+V +L +L+ VV
Sbjct: 121 LVTPNKTLHQLIYTWFSQKYVLMKKRSEDVQGRAIEILGTLRKAKGKAKVHALSELKQVV 180

Query: 181 AAHSSAKKMVVDNGGVAVISSLLGSFTSHAVGSEAVGILVNLDLDSELKNNLIQPTRRSL 240
            AH+ AKK VVD GGV VISSLL  FTSHAVGSEA+ ILVNL+LDS+ K  L+QP R SL
Sbjct: 181 MAHAIAKKTVVDEGGVFVISSLLSPFTSHAVGSEAIAILVNLELDSDSKAGLMQPARVSL 240

Query: 241 IADILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLLRMVKDQKHPNGVTAGL 300
           + D+LN+GSIETKINC + I ++++    R E VSS SLLVGL+R+VKD++  NGV+  L
Sbjct: 241 MVDMLNDGSIETKINCARLIGRLVEEKGFRAELVSSHSLLVGLMRLVKDRRRRNGVSPAL 300

Query: 301 SLLRSFCSNESIKKQLVSIGAVAKLGGALSSFNGDGLESALYILDGLSCLPEGRLALKDC 360
           +LL+S   ++ ++  LV IGAV +L   L   + + LESAL++LD L    EGR+ALKD 
Sbjct: 301 TLLKSVSVHKQVRNLLVRIGAVPQLVDVLPCLDVECLESALFVLDSLCLESEGRIALKDS 360

Query: 361 EETIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAVVAVDAGLAAKLLLVIQSGCNK 420
             TI   V+LLMKVSE CT +A+SILW+V K+A EEC+ +AV+ GLAAKLLLVIQSGC+ 
Sbjct: 361 VNTIPHTVRLLMKVSEKCTNYAISILWSVCKLASEECSSLAVEVGLAAKLLLVIQSGCDP 420

Query: 421 EVKQRSSELLKLCSLNYPDSIFISKCKLTTTIQ 451
            +KQRS+ELLKLCSL+Y DS+FISKCKLT TIQ
Sbjct: 421 ALKQRSAELLKLCSLHYSDSMFISKCKLTRTIQ 444

BLAST of CmaCh12G006050 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 154.1 bits (388), Expect = 2.7e-37
Identity = 127/406 (31.28%), Postives = 206/406 (50.74%), Query Frame = 0

Query: 53  LKKMIVELESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLG-HLTCPTTM 112
           + + I  L+  I++P  F CPISLE MQDPVT+C+GQTY+R +I+ W S+G + TCP T 
Sbjct: 1   MPRNIEPLDLGIQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTR 60

Query: 113 QELWDDSLTPNRTLHQLIHTWF------SQKFLAFKKKSEDVQGRVTELLDSLKKVKG-- 172
             L D +L PN TL +LI  W         + +   K+  D    V  LL     + G  
Sbjct: 61  APLSDFTLIPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPTS-VRALLSQASAITGTH 120

Query: 173 ---QARVQSLKDLRHVVAAHSSAKKMVV---DNGGVAVISSLLGSFTSHAVGSEAVGILV 232
              ++R  +L+ LR    A  S K  V+    N    +I  L    TS  + SE++ +LV
Sbjct: 121 VSVRSRAAALRRLRGF--ARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLV 180

Query: 233 NLDL--DSELKNNLIQPTRRSLIADILNEGSIETKINCTKFIKKIMDGD---DSRTESVS 292
            L +   ++  +    P R   +  +L + SIET++N    I+ +  G    D +    +
Sbjct: 181 MLPITEPNQFVSISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISN 240

Query: 293 SLSLLVGLLRMVKDQ-KHPNGVTAGLSLLRSFCSNESIKKQLVSIGAVAKLGGALSS-FN 352
           S S+  G+L ++++       +  G+  L + CS +S +   ++ GA   L   L++ F+
Sbjct: 241 SESVFEGVLDLLRNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFD 300

Query: 353 GDGLESALYILDGLSCLPEGRLALKDCEETIKMLVKLLMKVSEICTEFALSILWAVFKVA 412
               E AL  ++ L   PEG  A  +   T+ +LVK +++VS+  TE+A   L A+   A
Sbjct: 301 RCDTERALATVELLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC-TA 360

Query: 413 PEECAVVAVDAGLAAKLLLVIQSGCNKEVKQRSSELLKLCSLNYPD 437
            E     A  AG+  +LLL++QS C +  K+++ +LLKL   ++PD
Sbjct: 361 EERWREEAAGAGVVVQLLLMVQSECTERAKKKAQKLLKLLRDSWPD 402

BLAST of CmaCh12G006050 vs. TAIR 10
Match: AT1G49780.1 (plant U-box 26 )

HSP 1 Score: 146.7 bits (369), Expect = 4.4e-35
Identity = 115/401 (28.68%), Postives = 202/401 (50.37%), Query Frame = 0

Query: 60  LESSIEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSL 119
           L+  I++P  F CPISL+ M DPVT+ +GQTY+R +I  W ++G+ TCP T   L D +L
Sbjct: 8   LDLGIQIPYHFRCPISLDLMSDPVTISTGQTYDRTSIDSWIAMGNTTCPVTRVALSDFTL 67

Query: 120 TPNRTLHQLIHTWF-------SQKFLAFKKKSEDVQGRVTELLDSLKKVKG-----QARV 179
            PN TL +LI  W         ++    K+ ++ +   V  LL     + G     ++R 
Sbjct: 68  IPNHTLRRLIQEWCVANRSNGVERIPTPKQPADPIS--VRSLLSQASAITGTHVSVRSRA 127

Query: 180 QSLKDLRHVVAAHSSAKKMVVDNGGVAVISSLL------GSFTSHAVGSEAVGILVNLDL 239
            +++ LR +       + ++  +    ++  +L       S +S  V SE++ +LV L +
Sbjct: 128 AAIRRLRGLARDSEKNRVLIAGHNAREILVRILFADIETTSLSSELV-SESLALLVLLHM 187

Query: 240 -DSELKNNLIQPTRRSLIADILNEGSIETKINCTKFIKKIMDGDDSRTESV---SSLSLL 299
            ++E +     P+R   +  +L + SIE ++N    I+ ++ G  S    +    S S+ 
Sbjct: 188 TETECEAVASDPSRVGFMTRLLFDSSIEIRVNAAALIEMVLTGAKSMDLKLIISGSDSIF 247

Query: 300 VGLLRMVKDQ-KHPNGVTAGLSLLRSFCSNESIKKQLVSIGAVAKLGGALSS-FNGDGLE 359
            G+L ++K+       +  G+  + + C  +  +   +S GA   L   L++ F+    E
Sbjct: 248 EGVLDLLKNPISSRRALKIGIKAIFALCLVKQTRHLAISAGAPGILIDRLAADFDRCDTE 307

Query: 360 SALYILDGLSCLPEGRLALKDCEETIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECA 419
             L  ++ L  LPEG  A  +   T+ ++VK +++VS+  TE+A   L A+   A E C 
Sbjct: 308 RGLATVELLCRLPEGCAAFGEHALTVPLMVKTILRVSDRATEYAAGALLALC-TAEERCR 367

Query: 420 VVAVDAGLAAKLLLVIQSGCNKEVKQRSSELLKLCSLNYPD 437
             A  AGL  +LLL++QS C +  K+++  LLKL   ++PD
Sbjct: 368 DEAAAAGLVTQLLLLVQSDCTERAKRKAQMLLKLLRDSWPD 404

BLAST of CmaCh12G006050 vs. TAIR 10
Match: AT5G64660.1 (CYS, MET, PRO, and GLY protein 2 )

HSP 1 Score: 141.4 bits (355), Expect = 1.8e-33
Identity = 126/398 (31.66%), Postives = 201/398 (50.50%), Query Frame = 0

Query: 64  IEVPSVFICPISLEPMQDPVTLCSGQTYERCNIQKWFSLGHLTCPTTMQELWDDSLTPNR 123
           I VP+ F CPISL+ M+ PV+LC+G TY+R +IQ+W   G+ TCP TMQ L +    PNR
Sbjct: 8   ITVPTFFRCPISLDVMKSPVSLCTGVTYDRASIQRWLDGGNNTCPATMQILQNKDFIPNR 67

Query: 124 TLHQLIHTWF-SQKFLAFKKKSEDVQGRVTELLDSLKKVKGQARVQSLKDLRHVVAAHSS 183
           TL +LI  W  S +     + +E       E+ D++ +VK +   +  +D R V++    
Sbjct: 68  TLQRLIEIWSDSVRRRTCVESAELAAPTRDEIADAIDRVKIE---KEERDDREVLSKIVR 127

Query: 184 AKKMVVDNGG------------VAVISSLLGSFTSHA---VGSEAVGILVNLD---LDSE 243
             +   DN G            V +I+ +    TS A   V  EAV IL  +     D  
Sbjct: 128 FGRESDDNRGFLAGKDDFVKLLVDLINQVDFETTSAAKSLVVQEAVKILSTIRSKVSDRR 187

Query: 244 LKNNLIQPT---RRSLIADILNEGSIETKINCTKFIKKIMDGDDSRTESVSSLSLLVGLL 303
             +NLI      R S+I  +   G++E KI+C   ++ I    +S+        L+  L+
Sbjct: 188 RFSNLILTNGRDRLSVIVYLFKTGNVELKIDCAGLLEFIAVDAESKLLIAERDGLITELM 247

Query: 304 RMVKDQKHPNGVTAGLSLLRSFCSNESIKKQLVS---IGAVAK-LGGALSSFNGDGLESA 363
           + +      + + + LS L +  S + +K  L+    IG V K L  + SS +    E  
Sbjct: 248 KSISKDSDLSLIESSLSCLIAISSPKRVKLNLLREKLIGDVTKLLSDSTSSLSVSVTEKC 307

Query: 364 LYILDGLSCLPEGRLALKDCE-ETIKMLVKLLMKVSEICTEFALSILWAVFKVAPEECAV 423
           L +L+ L+   EGR  +   + E +K +VK LMKVS   TE A+++LW+V  +  E+ A+
Sbjct: 308 LKLLEILASTKEGRSEICGGDGECLKTVVKKLMKVSTAATEHAVTVLWSVSYLFKEDKAL 367

Query: 424 VAV-DAGLAAKLLLVIQSGCNKEVKQRSSELLKLCSLN 434
            AV       K+LL++QS C+  V++  ++LLK+  +N
Sbjct: 368 EAVTSVNGVTKILLLLQSNCSPAVRRMLTDLLKVFKVN 402

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q058P42.2e-14861.56U-box domain-containing protein 30 OS=Arabidopsis thaliana OX=3702 GN=PUB30 PE=1... [more]
Q9FHN91.2e-14360.93U-box domain-containing protein 31 OS=Arabidopsis thaliana OX=3702 GN=PUB31 PE=2... [more]
Q10PI91.0e-12954.55U-box domain-containing protein 75 OS=Oryza sativa subsp. japonica OX=39947 GN=T... [more]
Q9LT793.8e-3631.28U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2... [more]
Q9FXA46.1e-3428.68U-box domain-containing protein 26 OS=Arabidopsis thaliana OX=3702 GN=PUB26 PE=2... [more]
Match NameE-valueIdentityDescription
A0A6J1KKN56.0e-250100.00RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111496578 P... [more]
A0A6J1GHN92.1e-23996.67RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111454038... [more]
A0A0A0LKY42.5e-21686.59RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_2G354100 PE... [more]
A0A5A7UYA87.4e-21686.81RING-type E3 ubiquitin transferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C... [more]
A0A1S3BA207.4e-21686.81RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103487822 PE=4 ... [more]
Match NameE-valueIdentityDescription
XP_023002832.11.2e-249100.00U-box domain-containing protein 30-like [Cucurbita maxima][more]
XP_022951085.14.4e-23996.67U-box domain-containing protein 30-like [Cucurbita moschata][more]
XP_023536979.14.9e-23896.23U-box domain-containing protein 30-like [Cucurbita pepo subsp. pepo][more]
KAG6585712.15.0e-23595.76U-box domain-containing protein 31, partial [Cucurbita argyrosperma subsp. soror... [more]
KAG7020615.18.3e-22294.87U-box domain-containing protein 31, partial [Cucurbita argyrosperma subsp. argyr... [more]
Match NameE-valueIdentityDescription
AT3G49810.11.5e-14961.56ARM repeat superfamily protein [more]
AT5G65920.18.8e-14560.93ARM repeat superfamily protein [more]
AT3G19380.12.7e-3731.28plant U-box 25 [more]
AT1G49780.14.4e-3528.68plant U-box 26 [more]
AT5G64660.11.8e-3331.66CYS, MET, PRO, and GLY protein 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR003613U box domainSMARTSM00504Ubox_2coord: 69..132
e-value: 3.6E-30
score: 116.2
IPR003613U box domainPFAMPF04564U-boxcoord: 66..136
e-value: 6.2E-16
score: 58.3
IPR003613U box domainPROSITEPS51698U_BOXcoord: 65..139
score: 36.861797
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 65..137
e-value: 5.1E-25
score: 89.3
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 138..446
e-value: 1.4E-21
score: 78.6
NoneNo IPR availablePANTHERPTHR22849WDSAM1 PROTEINcoord: 38..449
NoneNo IPR availablePANTHERPTHR22849:SF142U-BOX DOMAIN-CONTAINING PROTEIN 30coord: 38..449
NoneNo IPR availableCDDcd16664RING-Ubox_PUBcoord: 70..112
e-value: 8.9842E-25
score: 94.2226
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 63..136
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 151..436

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G006050.1CmaCh12G006050.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
molecular_function GO:0004842 ubiquitin-protein transferase activity