CmaCh12G005600 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G005600
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionRING/U-box superfamily protein
LocationCma_Chr12: 2921491 .. 2924877 (-)
RNA-Seq ExpressionCmaCh12G005600
SyntenyCmaCh12G005600
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCAAGGGCAAAGGAGTAGTGTTGGTTCATTGTCGGAAACCATAAACTTTGAACATGGATCTTCATCGAATAATCCTGGTAGCCAACCAGTTTACTGGAATAACATGTGGAATCCAGCAGAAAACCGAATACCGGATTATTTGTTGCCTACTACTGATATAAATACGGGATATGTAACCACGGTGAGCCATGAGCAACGGAATTTGAGTAGATGGAGCTTAGGTGAACCTAGCTCTTGTGATATGCAAACCGAGGCATTATCTGATGAACAAAAAGGTGAGGTTGGATGGTCTTCAGTGTCTCGTGATGCAGATGGTCCAGTTACAGAAACCCGACTCTGTGAGCCGTCAAATAATCCTACACTGGGTCATGTGAATTTCAGCCCAATGATTATACAAAATTCCAATTCTAATGCACTTCCTCATAGTCTAAATTTAAATGCAAGTTTTGCTAGTCATGGAGGTGAGAGTAGCCGAGTTAATGAAGGTTCTAACTTGTATAAATCTAATGGATCAGAAGAAGGACGGGCTCTATGTTCTAGTGCTTCGGATCCTCTTTTACTTCCTTCTGGAAATAGTGGACTTCCAGTGGTTGCAAATGATGGTAGAGCAGGATCTTCCTTGGATGGACGTCGAGCACCCTGTAAACGAAAAGCTATCGAGGGAAGTGTTACACAGCCCTCTTTAAGTGGAAGCAGTAACTATGCTCAGCATATTGAAAGTGGTGCTGAGCCCACTCTTCCAGTTTTTCCTGGTCGCTACAACACAGGAAGTAGGCTAACTATTCCTGCTCCACCAGCACGAATGAATCAAGCTCCTGTACGAGGTGCAGGTGAAATGACTTCCAACAGCTTCTCTGAGTCAATGGTTGCAGAAAGTTCAGATAGTTCTCAAAGAAACTATCGTATAAGGATTAGTTCATCCAATGCACAAGATTCTTTTGCACCGACTGGAATGACTGTTCGGCATCCCGGGGCCTCATCTTCCCAGCTGCCAGCTAGAATTCTACCTGCTGAGCATGTATTAGACTTGAGGCCAGCACCAGCTGTAGACAATATATCTGCTCAAGGTCAGCCACTTATGATACATGTGCCTGCTTTACCTCGAAGTCTGCAACCATATAGGTGGAATGGGAGCTCTACTTCAAGAAGTGGCAGTTCATCGAGCTCTGCAGGGGAGAGACAGGTGGTGCAACGTGAGGAGGTGAGACCAAGCAACATGGGAAGAAACGTATCTGAACATCCCATGTTTGTCCCAGCAAACGAGCTGAGGAATTTGGTCCGTAATCCATCAAGTAGAGGTTTAGCATCTTCAAACCTGTCCATTCCTGGAACTGTTGCTTCTACATCCAGGGTTGGTTCAAGTTCAGGGGTCCACCCCCCTTCTGGACCTTGGATGCCTCCTGAGAATAGTCCAACACAGTTTCCTAGAAGGTTAACAGAATATGTTCGTAGGCAGCTATTTTCTTCTGCTACAAATGAGTCTGGAGGTAGGACAAGTAATTATTCACAACGATCCGGTTCTACTTCTGCCCAGGATATGGTTCTTTCATCTGGATCTGGCCGTCGGGGGCACCATTTATTGCAACCAAGGTCCGCATTATGGATGGAAAGAAGAGGTGACAGTGGGCTTGGTCTTCCCTATTCATTACGAACCTTGGCTACTTCCGGTGAAGGAAGCGACAATAATAGACTAGTATCTGAGGTATGTTGAATTTTTCCTTCATGGAGTATCTGAATGACGCCCTAGTCGCATATGCGCTATATATCTTAATGCATATCTAAATCTTATTATCTCATCTTTGGATGCTGTACTATTTATATCGTGTCTTGTATCTTTGATTTATGATGGGTACATTTGTGCTGCATATCTATAATTACGTTTGTAGTAAGCGCCCTGGGGGGTTGTGCCTGCTTGTGGATGCTGGTGTTTGCTGGACGACTGCTTTTGAATTCTTTGAGTATCTTCTTCTGAAATGAATTTTGAAGCTCACATGTTATTGTTTTTTGTCTGCAGCAGATTCGCAATGTCTTGGGCCTTGTCCGCCGCGGGGAAAGCTTACGAGTTGAGGTAGTTTCCTTATCGGTGCCATATCATGTGCAATGGGTGCATTGGCGAATTTACATGATTGCATACAAGAAGTCATTATGCTACATGTTTGATTTTCGTCTCGATTTGTTAAAATGTGTAAATGGTAGCAAACAGCTACTTAGTCTTTGTTCATATTCCAGTTCAGTTGTGATAACTTAAAAGTGATATGAATATCCAATGTGATGGTGTATGACAGTGTCTTGTGTTTATAAACAAATGGTTCACTTCATTTTGCTCTTGAAGTAATAATCAAACATATTCTCTCAATATTCAAGGAGTGGATGATTTATGTATCAATATTAATGTTGCTTTAAAATTTTGTTTTGTTAGGATGTGATGATCCTGGACCAGTCACTATTTTTCGGGATGGCTGATATTTACGATCGGCACAGGGATATGCGCCTTGACGTTGACAACATGTCGTATGAGGTTCGACTGATACAAACCAACCGAACCCATTATTATTTCACATCTTTTTGTTGCTCTGTTAATCTATCAGTATGTCAAGTGCAGGAATTGTTGGCTTTGGAAGAGCGCATTGGAAACGTCAACACTGGACTAAGCGAAGAGACGATTGTTGCTCGACTGAAACGAACGAAGCACGTCAATGCCCCCGAACCACAGGTCGAGGAAGAACCATGTTGTGTTTGTCAGGTATCCACGAGTTCATACGTTTTCATTTGTATCGGTCTTTAAACCTGCAATAGCCTAAAACCTACCTGATTTCCAGGAGGAATACAAAGAAGGGGAAGACATTGGAATGCTGGACTGCGGGCACAAGTTCCACACACAATGTATCAAGCAATGGCTGATGCAAAAGAATATATGTCCCATCTGCAAAACAACTGGTTTTGCAAGCCGAGAATGAATGTTGGCTCGTAGAGTGAACATGGTGGCCCGTGGCTTGAACGAAGATAAGCGACCGAACAGGGGACGCCTCGAAGAGGGGGGATGCTAGGAAGTTGCTTGTCGATTCTCTACGTTCGGTCGGGAAATCGAAGAGCCACATTGAAGTTCTCTATATACATTCTACAAGTAGTAAAATTTTAAAATATGCAAGCAATCCATTTTTTGTTTTTTATTTTATTTTGGAAGAAAAATTCAAGGTTCTAATTGGCATGGAATTGTTAGCTTAAGGTTATGATGGTCAGTGAAACTAATTAATGTTTACTCCCATTGACATTAGAGATTGTTGGTCTTAATATATCATCGGACTTTCCCTTACTCAGCTCGGTTATTCACGGTCGTTTGGTTGAAGGTTTTGTTTAAAACGTCTTGGTGGGTAGGTAAGAATCG

mRNA sequence

ATGCAAGGGCAAAGGAGTAGTGTTGGTTCATTGTCGGAAACCATAAACTTTGAACATGGATCTTCATCGAATAATCCTGGTAGCCAACCAGTTTACTGGAATAACATGTGGAATCCAGCAGAAAACCGAATACCGGATTATTTGTTGCCTACTACTGATATAAATACGGGATATGTAACCACGGTGAGCCATGAGCAACGGAATTTGAGTAGATGGAGCTTAGGTGAACCTAGCTCTTGTGATATGCAAACCGAGGCATTATCTGATGAACAAAAAGGTGAGGTTGGATGGTCTTCAGTGTCTCGTGATGCAGATGGTCCAGTTACAGAAACCCGACTCTGTGAGCCGTCAAATAATCCTACACTGGGTCATGTGAATTTCAGCCCAATGATTATACAAAATTCCAATTCTAATGCACTTCCTCATAGTCTAAATTTAAATGCAAGTTTTGCTAGTCATGGAGGTGAGAGTAGCCGAGTTAATGAAGGTTCTAACTTGTATAAATCTAATGGATCAGAAGAAGGACGGGCTCTATGTTCTAGTGCTTCGGATCCTCTTTTACTTCCTTCTGGAAATAGTGGACTTCCAGTGGTTGCAAATGATGGTAGAGCAGGATCTTCCTTGGATGGACGTCGAGCACCCTGTAAACGAAAAGCTATCGAGGGAAGTGTTACACAGCCCTCTTTAAGTGGAAGCAGTAACTATGCTCAGCATATTGAAAGTGGTGCTGAGCCCACTCTTCCAGTTTTTCCTGGTCGCTACAACACAGGAAGTAGGCTAACTATTCCTGCTCCACCAGCACGAATGAATCAAGCTCCTGTACGAGGTGCAGGTGAAATGACTTCCAACAGCTTCTCTGAGTCAATGGTTGCAGAAAGTTCAGATAGTTCTCAAAGAAACTATCGTATAAGGATTAGTTCATCCAATGCACAAGATTCTTTTGCACCGACTGGAATGACTGTTCGGCATCCCGGGGCCTCATCTTCCCAGCTGCCAGCTAGAATTCTACCTGCTGAGCATGTATTAGACTTGAGGCCAGCACCAGCTGTAGACAATATATCTGCTCAAGGTCAGCCACTTATGATACATGTGCCTGCTTTACCTCGAAGTCTGCAACCATATAGGTGGAATGGGAGCTCTACTTCAAGAAGTGGCAGTTCATCGAGCTCTGCAGGGGAGAGACAGGTGGTGCAACGTGAGGAGGTGAGACCAAGCAACATGGGAAGAAACGTATCTGAACATCCCATGTTTGTCCCAGCAAACGAGCTGAGGAATTTGGTCCGTAATCCATCAAGTAGAGGTTTAGCATCTTCAAACCTGTCCATTCCTGGAACTGTTGCTTCTACATCCAGGGTTGGTTCAAGTTCAGGGGTCCACCCCCCTTCTGGACCTTGGATGCCTCCTGAGAATAGTCCAACACAGTTTCCTAGAAGGTTAACAGAATATGTTCGTAGGCAGCTATTTTCTTCTGCTACAAATGAGTCTGGAGGTAGGACAAGTAATTATTCACAACGATCCGGTTCTACTTCTGCCCAGGATATGGTTCTTTCATCTGGATCTGGCCGTCGGGGGCACCATTTATTGCAACCAAGGTCCGCATTATGGATGGAAAGAAGAGGTGACAGTGGGCTTGGTCTTCCCTATTCATTACGAACCTTGGCTACTTCCGGTGAAGGAAGCGACAATAATAGACTAGTATCTGAGCAGATTCGCAATGTCTTGGGCCTTGTCCGCCGCGGGGAAAGCTTACGAGTTGAGGATGTGATGATCCTGGACCAGTCACTATTTTTCGGGATGGCTGATATTTACGATCGGCACAGGGATATGCGCCTTGACGTTGACAACATGTCGTATGAGGAATTGTTGGCTTTGGAAGAGCGCATTGGAAACGTCAACACTGGACTAAGCGAAGAGACGATTGTTGCTCGACTGAAACGAACGAAGCACGTCAATGCCCCCGAACCACAGGTCGAGGAAGAACCATGTTGTGTTTGTCAGGAGGAATACAAAGAAGGGGAAGACATTGGAATGCTGGACTGCGGGCACAAGTTCCACACACAATGTATCAAGCAATGGCTGATGCAAAAGAATATATGTCCCATCTGCAAAACAACTGGTTTTGCAAGCCGAGAATGAATGTTGGCTCGTAGAGTGAACATGGTGGCCCGTGGCTTGAACGAAGATAAGCGACCGAACAGGGGACGCCTCGAAGAGGGGGGATGCTAGGAAGTTGCTTGTCGATTCTCTACGTTCGGTCGGGAAATCGAAGAGCCACATTGAAGTTCTCTATATACATTCTACAAGTAGTAAAATTTTAAAATATGCAAGCAATCCATTTTTTGTTTTTTATTTTATTTTGGAAGAAAAATTCAAGGTTCTAATTGGCATGGAATTGTTAGCTTAAGGTTATGATGGTCAGTGAAACTAATTAATGTTTACTCCCATTGACATTAGAGATTGTTGGTCTTAATATATCATCGGACTTTCCCTTACTCAGCTCGGTTATTCACGGTCGTTTGGTTGAAGGTTTTGTTTAAAACGTCTTGGTGGGTAGGTAAGAATCG

Coding sequence (CDS)

ATGCAAGGGCAAAGGAGTAGTGTTGGTTCATTGTCGGAAACCATAAACTTTGAACATGGATCTTCATCGAATAATCCTGGTAGCCAACCAGTTTACTGGAATAACATGTGGAATCCAGCAGAAAACCGAATACCGGATTATTTGTTGCCTACTACTGATATAAATACGGGATATGTAACCACGGTGAGCCATGAGCAACGGAATTTGAGTAGATGGAGCTTAGGTGAACCTAGCTCTTGTGATATGCAAACCGAGGCATTATCTGATGAACAAAAAGGTGAGGTTGGATGGTCTTCAGTGTCTCGTGATGCAGATGGTCCAGTTACAGAAACCCGACTCTGTGAGCCGTCAAATAATCCTACACTGGGTCATGTGAATTTCAGCCCAATGATTATACAAAATTCCAATTCTAATGCACTTCCTCATAGTCTAAATTTAAATGCAAGTTTTGCTAGTCATGGAGGTGAGAGTAGCCGAGTTAATGAAGGTTCTAACTTGTATAAATCTAATGGATCAGAAGAAGGACGGGCTCTATGTTCTAGTGCTTCGGATCCTCTTTTACTTCCTTCTGGAAATAGTGGACTTCCAGTGGTTGCAAATGATGGTAGAGCAGGATCTTCCTTGGATGGACGTCGAGCACCCTGTAAACGAAAAGCTATCGAGGGAAGTGTTACACAGCCCTCTTTAAGTGGAAGCAGTAACTATGCTCAGCATATTGAAAGTGGTGCTGAGCCCACTCTTCCAGTTTTTCCTGGTCGCTACAACACAGGAAGTAGGCTAACTATTCCTGCTCCACCAGCACGAATGAATCAAGCTCCTGTACGAGGTGCAGGTGAAATGACTTCCAACAGCTTCTCTGAGTCAATGGTTGCAGAAAGTTCAGATAGTTCTCAAAGAAACTATCGTATAAGGATTAGTTCATCCAATGCACAAGATTCTTTTGCACCGACTGGAATGACTGTTCGGCATCCCGGGGCCTCATCTTCCCAGCTGCCAGCTAGAATTCTACCTGCTGAGCATGTATTAGACTTGAGGCCAGCACCAGCTGTAGACAATATATCTGCTCAAGGTCAGCCACTTATGATACATGTGCCTGCTTTACCTCGAAGTCTGCAACCATATAGGTGGAATGGGAGCTCTACTTCAAGAAGTGGCAGTTCATCGAGCTCTGCAGGGGAGAGACAGGTGGTGCAACGTGAGGAGGTGAGACCAAGCAACATGGGAAGAAACGTATCTGAACATCCCATGTTTGTCCCAGCAAACGAGCTGAGGAATTTGGTCCGTAATCCATCAAGTAGAGGTTTAGCATCTTCAAACCTGTCCATTCCTGGAACTGTTGCTTCTACATCCAGGGTTGGTTCAAGTTCAGGGGTCCACCCCCCTTCTGGACCTTGGATGCCTCCTGAGAATAGTCCAACACAGTTTCCTAGAAGGTTAACAGAATATGTTCGTAGGCAGCTATTTTCTTCTGCTACAAATGAGTCTGGAGGTAGGACAAGTAATTATTCACAACGATCCGGTTCTACTTCTGCCCAGGATATGGTTCTTTCATCTGGATCTGGCCGTCGGGGGCACCATTTATTGCAACCAAGGTCCGCATTATGGATGGAAAGAAGAGGTGACAGTGGGCTTGGTCTTCCCTATTCATTACGAACCTTGGCTACTTCCGGTGAAGGAAGCGACAATAATAGACTAGTATCTGAGCAGATTCGCAATGTCTTGGGCCTTGTCCGCCGCGGGGAAAGCTTACGAGTTGAGGATGTGATGATCCTGGACCAGTCACTATTTTTCGGGATGGCTGATATTTACGATCGGCACAGGGATATGCGCCTTGACGTTGACAACATGTCGTATGAGGAATTGTTGGCTTTGGAAGAGCGCATTGGAAACGTCAACACTGGACTAAGCGAAGAGACGATTGTTGCTCGACTGAAACGAACGAAGCACGTCAATGCCCCCGAACCACAGGTCGAGGAAGAACCATGTTGTGTTTGTCAGGAGGAATACAAAGAAGGGGAAGACATTGGAATGCTGGACTGCGGGCACAAGTTCCACACACAATGTATCAAGCAATGGCTGATGCAAAAGAATATATGTCCCATCTGCAAAACAACTGGTTTTGCAAGCCGAGAATGA

Protein sequence

MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVTTVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNPTLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIESGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRNYRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPANELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLTEYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRGDSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEEPCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE
Homology
BLAST of CmaCh12G005600 vs. ExPASy Swiss-Prot
Match: Q9FMM4 (Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RHG1A PE=2 SV=1)

HSP 1 Score: 370.9 bits (951), Expect = 3.2e-101
Identity = 278/728 (38.19%), Postives = 396/728 (54.40%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQG+R+S+G LSE +NFEHGSSS+N      +W N+ +  +N + DY++  ++ NT    
Sbjct: 1   MQGERASLGYLSEALNFEHGSSSSNGVID--HWENIHSLGDNDLQDYMIANSESNTSLAN 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           +V HEQ+ L R+SLGE SS   + EA S  ++                 ETR  +   N 
Sbjct: 61  SVYHEQQGLRRFSLGEASSSGTKDEASSHNEQR---------------METRCFDGRGNE 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKS-NGSEEGRALC 180
               ++  P+  Q S +N    ++NLNA +         ++E  N Y+  +G  E     
Sbjct: 121 I---IDLDPVFAQPSGTNQPVQNVNLNAEYI-------EIHEDINPYRGRSGFIEANGPG 180

Query: 181 SSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHI 240
           +  S P        G     N    GSS++GRRA CKRKA+EGS++Q S  G  ++ +  
Sbjct: 181 TRVSQP--------GRSFEENGVGTGSSVEGRRASCKRKALEGSISQSSSGGYHDFQRGE 240

Query: 241 ESGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQR 300
            S   P   VF      G+ L I        +  V G       S     V+  ++SS R
Sbjct: 241 SSSWTPGSTVF----RPGNGLNISGSLDNGPRGMVSGTVPNFPVSAPNFPVSAIAESSSR 300

Query: 301 NYRIRISSSNAQDSFAPT----GMTVRHPGASSSQLPARILPA-EHVLDLRPAPA-VDNI 360
           N  +R + S+ Q++  P+    G  VR P   S    +R LPA +H LDLRP  + V + 
Sbjct: 301 NICVRSNPSDHQETVNPSTFAAGTVVRRPVPPSQLNLSRHLPADQHSLDLRPGQSFVVSR 360

Query: 361 SAQGQPLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSE 420
           +    P+ I  P     L P+RW GSS    G+S+S+A   + +  +E R  ++  N  E
Sbjct: 361 NPNSTPVSI-PPGSRTMLPPFRWTGSSLV-GGTSNSTAPVERNLHLDETRSRSIPGNTLE 420

Query: 421 HPMFVPANELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGP-----WMPP 480
            PMF  A E+ N  R+ SSR + + NL+   + +S SR GS++ V PP  P     W   
Sbjct: 421 IPMFA-APEVGNFARSQSSRNVTNGNLN---SASSVSRTGSTTSVPPPPPPSSNLAWTSY 480

Query: 481 ENSPTQFPRRL--TEYVRRQLFSS----ATNESGGRTSNYSQRSGSTSAQDMVLS-SGSG 540
           +NSP    RR   +E  RR L SS    ATN+  G        +   S+  +VL   G  
Sbjct: 481 QNSPHYQRRRTERSELARRSLLSSLAADATNQRSGDHPTLRSLAPPASSDGLVLQPGGDN 540

Query: 541 RRGHHLLQPRSALWMERRGDSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRR-- 600
            + H+    R+    +R+GDS +G+P+ LR LA +  G   +RL+  Q++NVL ++RR  
Sbjct: 541 SQMHNRAYSRAGPLFDRQGDSVVGIPHPLRALAAASRG--RSRLMVSQMQNVLDVMRRDA 600

Query: 601 -GESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEE 660
              +LR+EDVM+L+ S+ F  A  +DR+RDMRLDVDNMSYEELLALEERIG+V TG++EE
Sbjct: 601 NNNNLRLEDVMLLNHSVLFDGATGHDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEE 660

Query: 661 TIVARLKRTKHVNAPEPQVEEEPCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNI 707
           TI  RLK+ K+ +  +   + EPCCVCQEEY EGED+G L+CGH+FH+QCIK+WL QKN+
Sbjct: 661 TISNRLKQRKYKSNTKSPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKNL 681

BLAST of CmaCh12G005600 vs. ExPASy Swiss-Prot
Match: O49500 (E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV=1)

HSP 1 Score: 294.3 bits (752), Expect = 3.8e-78
Identity = 254/727 (34.94%), Postives = 354/727 (48.69%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQG RS+ G  S  IN+  G    +  S+    NN+ NP + + P+    T      Y +
Sbjct: 1   MQGPRST-GDSSTGINYADGEPICSTNSETTS-NNILNPVDVQFPNN--TTGSGRPTYAS 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           + SH  +N + WS GE SS    ++ L               +++G  T+ +L       
Sbjct: 61  SSSHVVQNHNWWSFGESSSRLGPSDHL---------------NSNGSKTDRQLLSDGYG- 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
                       +  +   LP    L  S +SH    S VN G ++   +G +    +  
Sbjct: 121 ----------FEEGQSGMLLPGESFLRGSSSSH--MLSHVNLGKDMDIGSGLQTSGVVIR 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
             +    L  G+S           GSSL G  + CKRKA+EG+ +      S       E
Sbjct: 181 HNNCETSL--GSSSQTAEERSSGPGSSLGGLGSSCKRKALEGAPSHSFPGESHGCFFQTE 240

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAP----PARMNQA-------PVRGAGEMTSNSFSESM 300
           +GA         +Y+  S L++  P    P   NQ+        + G   +T+++F  + 
Sbjct: 241 NGA---WNEGLAQYDASSSLSLSMPSQNSPNVNNQSGLPEPRFGLGGGRAVTASAFPSTR 300

Query: 301 VAESSDSSQRNYRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPA 360
             E+     R         +   SF  +G +VR       QLPA   P    LD R  P 
Sbjct: 301 STETISRPGRRLNPGQPPESVAFSFTQSGSSVR----QQQQLPA-TSPFVDPLDARAIPV 360

Query: 361 V-DNISAQGQPLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMG 420
              + S  GQP MIH+PAL R++  + W+ SS+SR+ S          +  E + P +  
Sbjct: 361 TGSSSSGDGQPSMIHLPALTRNIHQFAWSASSSSRANS----------MPEEGLSPWDAP 420

Query: 421 RNVSEHPMF-VPANELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVH--PPSGPW 480
           R  SE P+F  PANE RN V++         N S  G      R GSSSG+H   P+  W
Sbjct: 421 RINSEQPVFTTPANETRNPVQDQFCWSFTRGNPSTSGDSPFVPRAGSSSGIHGLQPNPTW 480

Query: 481 MPPENSPTQFPRRLTEYVRRQLFSSATNESG--GRTSNYSQRSGSTSAQDMVLSSGSGRR 540
           + P N       R++E     LF S  +ES   G +        S S+ +    SGS  R
Sbjct: 481 VTPHNQ-----SRISEVAPWSLFPSIESESATHGASLPLLPTGPSVSSNEAAAPSGSSSR 540

Query: 541 GHHLLQPRSALWMERRGDSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESL 600
            H   Q RS L +ER+ D  L L +  R+LA   +G   NRL+SE IR VL  +RRGE+L
Sbjct: 541 SHRSRQRRSGLLLERQNDH-LHLRHLGRSLAADNDG--RNRLISE-IRQVLSAMRRGENL 600

Query: 601 RVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVAR 660
           R ED M+ D  ++ GMA+++DRHRDMRLDVDNMSYEELLAL ERIG+V+TGLSEE I+  
Sbjct: 601 RFEDYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKV 660

Query: 661 LKRTKHV-NAPEPQVEEEPCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPIC 710
           +K+ KH  +A     + EPCCVCQEEY EG+D+G L CGH+FHT C+KQWLM KN+CPIC
Sbjct: 661 MKQHKHTSSAAGSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPIC 666

BLAST of CmaCh12G005600 vs. ExPASy Swiss-Prot
Match: Q7XTV7 (Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=39947 GN=HIP1 PE=1 SV=2)

HSP 1 Score: 290.0 bits (741), Expect = 7.1e-77
Identity = 253/741 (34.14%), Postives = 371/741 (50.07%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNP-GSQPVYWNNMWNPAE-NRIPDYLLPTTDINTGY 60
           MQGQ++SV  L++   F+H SSS NP   Q  YWNN+    E + +  Y +  +D    Y
Sbjct: 1   MQGQKNSVEQLADVFGFDHASSSGNPVMDQQGYWNNILGSVESHNLQGYQVNRSDGTIPY 60

Query: 61  VTTVSHEQRNLSRWSLGEPSSCDMQTE-ALSDEQKGEVGWSSVSRDADG--PVTETRLCE 120
              V      L  W  GE S+         S+E K E       R+  G   + E RL  
Sbjct: 61  GNGVHQNGTFLGFWESGEASASGSSLHFGGSNEIKAE------QRNIGGGLRIGERRLVA 120

Query: 121 PSN----NPTLG-HVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNE------GS 180
             N    N  +G ++N + +  +NSN N       L+    SH G + + +E       +
Sbjct: 121 ERNLSLDNVDIGLNINGNDLSGENSNVNGASQGSELHGG-CSHTGSNGQASELRLHPYRT 180

Query: 181 NLYKSNGSEEGRALCSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSV 240
            +  ++  E   +L  S +     P G+  L     D R GSSLDGRR  CKRK IEG  
Sbjct: 181 FILGADQPEPFNSLNGSEN-----PLGDFSLMPEGIDQRPGSSLDGRRLACKRKNIEGVN 240

Query: 241 TQPSLSGSSNYAQHIESGAEPTLPVFPGRYNTGSRLTIPAP-----PARMN-QAPVRGA- 300
            Q S   S++++   +S       +    +N      +P+P     P+ ++ Q P  GA 
Sbjct: 241 GQCSAGASTSFSHRNDSIFH---NIASSSHNPSPSTNLPSPNCLLVPSTLDEQLPRYGAT 300

Query: 301 -GEMTSNSFSESMVAESSDSSQRNYRIRISSSNAQDSFA--PTGMTVRHPGASSSQLPAR 360
              ++S+S+  S    +S  SQR++R R S +     +   P+  T+RH  + + Q P  
Sbjct: 301 TAGLSSSSYDPSGGNNNSGGSQRSFRPRTSLAQHIGPYGVWPSSSTIRHSNSWNHQPP-- 360

Query: 361 ILPAEHVLDLRP--APAVDNISAQGQPLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAG 420
             P +   D  P   P V +++ Q Q  M  VP +P+    +   G+S+SR+G+      
Sbjct: 361 --PFQSSFDEPPEVIPVVSSLNFQYQHPMNVVPGIPQMSHRFTGPGASSSRTGNL----- 420

Query: 421 ERQVVQREEVRPSNMGRNVSEHPMFVP--ANELRNLVRNPSSRGLASSNLSIPGTVASTS 480
           E +++  EE    N+    +  P  VP  A ++R+L+  PSS  +     +IPG V S+S
Sbjct: 421 ENRIIGSEEFSARNV--VATSFPDAVPPAALDMRHLIPEPSSWNVDGRATTIPGNVPSSS 480

Query: 481 RVGSSSGVHPPSG-PWMPPENSPTQFPRRLTEYVRRQLFSSATNESGGRTSNYSQRSGST 540
           R  ++S V+PP+G P++  +N   + PR L+E +                   S+ SG+ 
Sbjct: 481 RANTNSMVNPPAGSPFIAHQNLHRRNPRNLSEEI-------------------SRLSGAL 540

Query: 541 SAQDMVLSSGSGRRGHHLLQPRSALWMERRGDSGLGLPYSLRTLATSGEGSDNNRLVSEQ 600
                        RGH   + RS   +ER+GD   G+P S R+        +  RL+  +
Sbjct: 541 -------------RGHQHPRLRSGFLLERQGDGVWGVPLSTRS-------REGRRLI--E 600

Query: 601 IRNVLGLVRRGESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIG 660
           IRN L ++ RGE++R E       S+F+G  DI+DRHRDMRLD+DNMSYEELLALEERIG
Sbjct: 601 IRNALEMIHRGENVRFE-------SIFYGGVDIHDRHRDMRLDIDNMSYEELLALEERIG 660

Query: 661 NVNTGLSEETIVARLKRTKHVN-APEPQVEEEPCCVCQEEYKEGEDIGMLDCGHKFHTQC 710
           NV+TGLSEE +   LK+ K  +   E  VEEEPCC+CQEEY +G+D+G LDCGH FH  C
Sbjct: 661 NVSTGLSEEEVTKLLKQRKFSSWRLEASVEEEPCCICQEEYVDGDDLGTLDCGHDFHVGC 667

BLAST of CmaCh12G005600 vs. ExPASy Swiss-Prot
Match: Q9ZQF9 (E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV=1)

HSP 1 Score: 265.8 bits (678), Expect = 1.4e-69
Identity = 244/746 (32.71%), Postives = 356/746 (47.72%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQG R S+G  S  +N   G S           N M NPA+   P+   P+      Y +
Sbjct: 4   MQGPR-SIGGSSTEVNQVDGESIY---CTETSLNTMLNPADTGFPNNSTPSG--RPTYAS 63

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALS------------DEQKGEVGWSSVSRDA--DG 120
           + SH  ++ + W  GE SS    ++ ++            D     VG+ S  R    +G
Sbjct: 64  SSSHAAQDHTWWRFGESSSIPGPSDQVNSIGIKTSHQLPQDGTHHFVGYGSEGRQTGLNG 123

Query: 121 PVTETRLCEPSNNPTLGHVNFSPMIIQNSNSNALPHSLNLNASFASH--GGESSRVNEGS 180
            + +  +   S      H+   P  ++ S+SN +P  ++++    S     ++S V    
Sbjct: 124 MMVDGGVHAGS------HIRNVPSFLRGSSSNPMPQHVDMSMDMDSDNCNAQTSGVVIRH 183

Query: 181 NLYKSNGSEEGRALCSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSV 240
           N Y S+     +A   S+S P                    S   G  + CKRKA+EGS 
Sbjct: 184 NSYGSSLGSSVQAAGESSSGP-------------------ASPFGGWGSSCKRKALEGS- 243

Query: 241 TQPSLSGSSNYAQHIESGAEPTLPVFPGRYNTGSRLTIPAP-PARMNQAPVRGAGEMTSN 300
             PS   S      I             +Y   S L++  P  +  N     G  E    
Sbjct: 244 --PSHYFSGETPNRIVQTENSASHASLSQYGASSSLSLATPSQSSPNVTNHFGRTEQMFG 303

Query: 301 SFSESMVAESSDSSQRNYRI------RISSSNAQDSFAPT----GMTVRHPGASSSQLPA 360
           S     VA S+  S RN         R++    Q+S A +    G +VR  G+    LP 
Sbjct: 304 SGGGRAVAASAFHSTRNTDTLSRAGRRLNPRQPQESVAFSVSHGGTSVRPTGSLQQNLPL 363

Query: 361 RILPAEHVLDLRPAPAVD-NISAQGQPLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAG 420
              P     D+R +     + + + Q  ++H+PAL R++  Y W+ S +SR+ S+ S  G
Sbjct: 364 N-SPFVDPPDVRSSSITSGSNTGENQTNIVHLPALTRNIHQYAWDASFSSRA-SNPSGIG 423

Query: 421 ERQVVQREEVRPS-NMGRNVSEHPMFVPANELRNLVRN-----PSSRGLASSNLSIPGTV 480
               +  E + P     R+  E P+F PA ++R  V +       S G +  +L +P   
Sbjct: 424 ----MPAERLGPQWETPRSNQEQPLFAPATDMRQPVHDLWNFARGSPGSSVDSLFVP--- 483

Query: 481 ASTSRVGSSSGVH--PPSGPWMPPENSPTQFPRRLTEYVRRQLFSS--ATNESGGRTSNY 540
               R G SS +H   P+  W+PP+N+P   P R +E     LF S  + + S G     
Sbjct: 484 ----RAGPSSAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIESPSASHGGPLPL 543

Query: 541 SQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRGDSGLGLPYSLRTLATSGEGSDN 600
                S S+ ++ + S S  R H     RS L +ER+ +  L L +  R+LA  G G   
Sbjct: 544 LPAGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNEL-LHLRHIGRSLAADGNG--R 603

Query: 601 NRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELL 660
           N+++SE IR VL  +RRGE+LRVED M+ D  ++ GM D++DRHR+MRLDVDNMSYEELL
Sbjct: 604 NQIISE-IRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELL 663

Query: 661 ALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVE----EEPCCVCQEEYKEGEDIGML 705
           AL ERIG+V+TGLSEE I+  +K+ KH ++    VE     EPCC+CQEEY EG+++G L
Sbjct: 664 ALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQEEYVEGDNLGTL 698

BLAST of CmaCh12G005600 vs. ExPASy Swiss-Prot
Match: Q5QLR5 (Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=39947 GN=ZFP1 PE=1 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 2.2e-33
Identity = 68/132 (51.52%), Postives = 93/132 (70.45%), Query Frame = 0

Query: 586 EDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLK 645
           E   I+D S  + ++++ D HRDMRLD+D+M+YEELLALEE+IG+VNTGL++  IV +LK
Sbjct: 387 EHATIMDFSRLYEVSNVVDEHRDMRLDIDSMTYEELLALEEQIGDVNTGLAKSYIVEKLK 446

Query: 646 RTKHV--------NAPEPQVEEEPCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKN 705
            +  V         + E  +E + C +CQEEY+  E IG LDCGH++H  CIKQWLM KN
Sbjct: 447 TSLFVPGSSCMSNKSSESSMENDACIICQEEYQVKECIGTLDCGHRYHEDCIKQWLMVKN 506

Query: 706 ICPICKTTGFAS 710
           +CPICKTT  ++
Sbjct: 507 LCPICKTTALST 518

BLAST of CmaCh12G005600 vs. ExPASy TrEMBL
Match: A0A6J1KSD9 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496039 PE=4 SV=1)

HSP 1 Score: 1394.4 bits (3608), Expect = 0.0e+00
Identity = 711/711 (100.00%), Postives = 711/711 (100.00%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP
Sbjct: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
           TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS
Sbjct: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300
           SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360
           YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 712
           PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 711

BLAST of CmaCh12G005600 vs. ExPASy TrEMBL
Match: A0A6J1KMU0 (probable E3 ubiquitin-protein ligase RHG1A isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496039 PE=4 SV=1)

HSP 1 Score: 1388.2 bits (3592), Expect = 0.0e+00
Identity = 710/711 (99.86%), Postives = 710/711 (99.86%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP
Sbjct: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
           TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS
Sbjct: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300
           SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360
           YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           DSGLGLPYSLRTLATSGEGSDNNRLVSE IRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSE-IRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 712
           PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 710

BLAST of CmaCh12G005600 vs. ExPASy TrEMBL
Match: A0A6J1GIQ0 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454242 PE=4 SV=1)

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 704/711 (99.02%), Postives = 710/711 (99.86%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYV+
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVS 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           +VSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSV+RDADGPVTETRLCEPSNNP
Sbjct: 61  SVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVARDADGPVTETRLCEPSNNP 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
           TLGHVNFSP+IIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKS+GSEEGRALCS
Sbjct: 121 TLGHVNFSPLIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSSGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300
           SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360
           YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPRSLQPYRWNGSSTSRSG SSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGGSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           D+GLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DTGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 712
           PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 711

BLAST of CmaCh12G005600 vs. ExPASy TrEMBL
Match: A0A6J1GHK1 (probable E3 ubiquitin-protein ligase RHG1A isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454242 PE=4 SV=1)

HSP 1 Score: 1378.2 bits (3566), Expect = 0.0e+00
Identity = 703/711 (98.87%), Postives = 709/711 (99.72%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYV+
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVS 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           +VSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSV+RDADGPVTETRLCEPSNNP
Sbjct: 61  SVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVARDADGPVTETRLCEPSNNP 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
           TLGHVNFSP+IIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKS+GSEEGRALCS
Sbjct: 121 TLGHVNFSPLIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSSGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300
           SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360
           YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPRSLQPYRWNGSSTSRSG SSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGGSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           D+GLGLPYSLRTLATSGEGSDNNRLVSE IRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DTGLGLPYSLRTLATSGEGSDNNRLVSE-IRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 712
           PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 710

BLAST of CmaCh12G005600 vs. ExPASy TrEMBL
Match: A0A0A0LND7 (RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356630 PE=4 SV=1)

HSP 1 Score: 1244.2 bits (3218), Expect = 0.0e+00
Identity = 625/713 (87.66%), Postives = 673/713 (94.39%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQGQRSS+GSLSETINFEHGSSSNNPG+QPVYWNN+WNPAENRIPDYLLPT+D+N+GYV+
Sbjct: 1   MQGQRSSIGSLSETINFEHGSSSNNPGNQPVYWNNVWNPAENRIPDYLLPTSDVNSGYVS 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           +VSHEQR+LSRWSLGEPSSCDMQTE L DEQK E+GWSS++RDADGPVTE RLCEPSNNP
Sbjct: 61  SVSHEQRSLSRWSLGEPSSCDMQTEMLPDEQKAEIGWSSMARDADGPVTENRLCEPSNNP 120

Query: 121 TLGHVNFSPMIIQ--NSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRAL 180
           +LGHVN SP+IIQ  NSNSNA+PH+LNLN+SF SHGG++SRVNEG+N+YKS+G EEGR L
Sbjct: 121 SLGHVNLSPLIIQNSNSNSNAIPHNLNLNSSFVSHGGDNSRVNEGTNVYKSSGPEEGRIL 180

Query: 181 CSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQH 240
           CS+ASDPLLLPSGNSGLPVV NDGR GSSLDGRRAPCKRK+IEG+V Q SLSGSSNY+QH
Sbjct: 181 CSNASDPLLLPSGNSGLPVVGNDGRPGSSLDGRRAPCKRKSIEGNVAQSSLSGSSNYSQH 240

Query: 241 IESGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQ 300
           IESG+E  LPVFPGRYNTGSRL+IP PPARMNQAPVRGAGE+TSNSFSES+VAESSDSSQ
Sbjct: 241 IESGSEAHLPVFPGRYNTGSRLSIPPPPARMNQAPVRGAGELTSNSFSESIVAESSDSSQ 300

Query: 301 RNYRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQ 360
           RNYRIRISSSNA DSFA  G  VRHPG  SSQLP R+LPAEH+LDLRPAPAVDN+SAQGQ
Sbjct: 301 RNYRIRISSSNAPDSFASAGTAVRHPGPPSSQLPGRLLPAEHILDLRPAPAVDNVSAQGQ 360

Query: 361 PLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFV 420
           P+MIHVPALPRSLQPYRWNGSS+SRSGSSSSSAGERQVVQREEVRPS+MGRNVSEHPMFV
Sbjct: 361 PIMIHVPALPRSLQPYRWNGSSSSRSGSSSSSAGERQVVQREEVRPSSMGRNVSEHPMFV 420

Query: 421 PANELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRR 480
           PANELR LVR+PSSR LASSNLSIPG VASTSRVGSSSGVHP SGPW+PPE+SPTQFPRR
Sbjct: 421 PANELRGLVRSPSSRALASSNLSIPGNVASTSRVGSSSGVHPSSGPWIPPESSPTQFPRR 480

Query: 481 LTEYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMER 540
           LTEYVRRQLF+SATNESGGRTSNYSQRSGSTSAQ+MVLSSGSGRRGHHLLQPRSALWMER
Sbjct: 481 LTEYVRRQLFASATNESGGRTSNYSQRSGSTSAQEMVLSSGSGRRGHHLLQPRSALWMER 540

Query: 541 RGDSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFG 600
           RGDSGLGLPYSLRTLATS EGSDNNRLVSE +RNVLGLVRRGESLRVEDVMILDQSLFFG
Sbjct: 541 RGDSGLGLPYSLRTLATSAEGSDNNRLVSEHLRNVLGLVRRGESLRVEDVMILDQSLFFG 600

Query: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVE 660
           MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGL+EETIVARLK+ K VNA + QVE
Sbjct: 601 MADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLNEETIVARLKQKKRVNAVDSQVE 660

Query: 661 EEPCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 712
           EEPCCVCQEEY +GEDIG L+CGH FHT CIKQWLMQKN+CPICKTTG ASRE
Sbjct: 661 EEPCCVCQEEYVDGEDIGTLECGHDFHTACIKQWLMQKNLCPICKTTGLASRE 713

BLAST of CmaCh12G005600 vs. NCBI nr
Match: XP_023002037.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita maxima] >XP_023002038.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita maxima] >XP_023002039.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita maxima])

HSP 1 Score: 1394.4 bits (3608), Expect = 0.0e+00
Identity = 711/711 (100.00%), Postives = 711/711 (100.00%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP
Sbjct: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
           TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS
Sbjct: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300
           SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360
           YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 712
           PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 711

BLAST of CmaCh12G005600 vs. NCBI nr
Match: XP_023002040.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X2 [Cucurbita maxima])

HSP 1 Score: 1388.2 bits (3592), Expect = 0.0e+00
Identity = 710/711 (99.86%), Postives = 710/711 (99.86%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP
Sbjct: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
           TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS
Sbjct: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300
           SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360
           YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           DSGLGLPYSLRTLATSGEGSDNNRLVSE IRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSE-IRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 712
           PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 710

BLAST of CmaCh12G005600 vs. NCBI nr
Match: XP_022951413.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >XP_022951414.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >XP_022951415.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >XP_022951416.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >KAG7020582.1 putative E3 ubiquitin-protein ligase RHG1A, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1384.4 bits (3582), Expect = 0.0e+00
Identity = 704/711 (99.02%), Postives = 710/711 (99.86%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYV+
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVS 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           +VSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSV+RDADGPVTETRLCEPSNNP
Sbjct: 61  SVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVARDADGPVTETRLCEPSNNP 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
           TLGHVNFSP+IIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKS+GSEEGRALCS
Sbjct: 121 TLGHVNFSPLIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSSGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300
           SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360
           YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360

Query: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPRSLQPYRWNGSSTSRSG SSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGGSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           D+GLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DTGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 712
           PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 711

BLAST of CmaCh12G005600 vs. NCBI nr
Match: KAG6585677.1 (putative E3 ubiquitin-protein ligase RHG1A, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 700/711 (98.45%), Postives = 709/711 (99.72%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQGQRSSVGSLSETINFEHGSSSNNPGSQP YWNNMWNPAENRIPDYLLPTTDINTGYV+
Sbjct: 286 MQGQRSSVGSLSETINFEHGSSSNNPGSQPAYWNNMWNPAENRIPDYLLPTTDINTGYVS 345

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           +VSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSV+RDADGPVTETRLCEPSNNP
Sbjct: 346 SVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVARDADGPVTETRLCEPSNNP 405

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
           TLGHVNFSP+IIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKS+GSEEGRALCS
Sbjct: 406 TLGHVNFSPLIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSSGSEEGRALCS 465

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE
Sbjct: 466 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 525

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300
           SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN
Sbjct: 526 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 585

Query: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360
           YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARI+PAEHVLDLRPAPAVDNISAQGQPL
Sbjct: 586 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARIIPAEHVLDLRPAPAVDNISAQGQPL 645

Query: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPRSLQPYRWNGSSTSRSG SSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 646 MIHVPALPRSLQPYRWNGSSTSRSGGSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 705

Query: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT
Sbjct: 706 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 765

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG
Sbjct: 766 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 825

Query: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           D+GLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 826 DTGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 885

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE
Sbjct: 886 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 945

Query: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 712
           PCCVCQEEYKEG+DIG+LDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE
Sbjct: 946 PCCVCQEEYKEGDDIGVLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 996

BLAST of CmaCh12G005600 vs. NCBI nr
Match: XP_023536901.1 (probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536903.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536904.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pepo] >XP_023536905.1 probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1379.4 bits (3569), Expect = 0.0e+00
Identity = 700/711 (98.45%), Postives = 709/711 (99.72%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQGQRSSVGSLSETINFEHGSSSNNPGSQP YWNNMWNPAENRIPDYLLPTTDINTGYV+
Sbjct: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPAYWNNMWNPAENRIPDYLLPTTDINTGYVS 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           +VSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSV+RDADGPVTETRLCEPSNNP
Sbjct: 61  SVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVARDADGPVTETRLCEPSNNP 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
           +LGHVNFSP+IIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKS+GSEEGRALCS
Sbjct: 121 SLGHVNFSPLIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSSGSEEGRALCS 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
           SASDPLLLPSGNSGLPVVANDGRAGSS+DGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE
Sbjct: 181 SASDPLLLPSGNSGLPVVANDGRAGSSMDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300
           SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN
Sbjct: 241 SGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQRN 300

Query: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPAVDNISAQGQPL 360
           YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPA+DNISAQGQPL
Sbjct: 301 YRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPALDNISAQGQPL 360

Query: 361 MIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420
           MIHVPALPRSLQPYRWNGSSTSRSG SSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA
Sbjct: 361 MIHVPALPRSLQPYRWNGSSTSRSGGSSSSAGERQVVQREEVRPSNMGRNVSEHPMFVPA 420

Query: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480
           NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT
Sbjct: 421 NELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGPWMPPENSPTQFPRRLT 480

Query: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540
           EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG
Sbjct: 481 EYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRG 540

Query: 541 DSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600
           D+GLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA
Sbjct: 541 DTGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMA 600

Query: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660
           DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE
Sbjct: 601 DIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVEEE 660

Query: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 712
           PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE
Sbjct: 661 PCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTGFASRE 711

BLAST of CmaCh12G005600 vs. TAIR 10
Match: AT5G42940.1 (RING/U-box superfamily protein )

HSP 1 Score: 370.9 bits (951), Expect = 2.2e-102
Identity = 278/728 (38.19%), Postives = 396/728 (54.40%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQG+R+S+G LSE +NFEHGSSS+N      +W N+ +  +N + DY++  ++ NT    
Sbjct: 1   MQGERASLGYLSEALNFEHGSSSSNGVID--HWENIHSLGDNDLQDYMIANSESNTSLAN 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           +V HEQ+ L R+SLGE SS   + EA S  ++                 ETR  +   N 
Sbjct: 61  SVYHEQQGLRRFSLGEASSSGTKDEASSHNEQR---------------METRCFDGRGNE 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKS-NGSEEGRALC 180
               ++  P+  Q S +N    ++NLNA +         ++E  N Y+  +G  E     
Sbjct: 121 I---IDLDPVFAQPSGTNQPVQNVNLNAEYI-------EIHEDINPYRGRSGFIEANGPG 180

Query: 181 SSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHI 240
           +  S P        G     N    GSS++GRRA CKRKA+EGS++Q S  G  ++ +  
Sbjct: 181 TRVSQP--------GRSFEENGVGTGSSVEGRRASCKRKALEGSISQSSSGGYHDFQRGE 240

Query: 241 ESGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQR 300
            S   P   VF      G+ L I        +  V G       S     V+  ++SS R
Sbjct: 241 SSSWTPGSTVF----RPGNGLNISGSLDNGPRGMVSGTVPNFPVSAPNFPVSAIAESSSR 300

Query: 301 NYRIRISSSNAQDSFAPT----GMTVRHPGASSSQLPARILPA-EHVLDLRPAPA-VDNI 360
           N  +R + S+ Q++  P+    G  VR P   S    +R LPA +H LDLRP  + V + 
Sbjct: 301 NICVRSNPSDHQETVNPSTFAAGTVVRRPVPPSQLNLSRHLPADQHSLDLRPGQSFVVSR 360

Query: 361 SAQGQPLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMGRNVSE 420
           +    P+ I  P     L P+RW GSS    G+S+S+A   + +  +E R  ++  N  E
Sbjct: 361 NPNSTPVSI-PPGSRTMLPPFRWTGSSLV-GGTSNSTAPVERNLHLDETRSRSIPGNTLE 420

Query: 421 HPMFVPANELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHPPSGP-----WMPP 480
            PMF  A E+ N  R+ SSR + + NL+   + +S SR GS++ V PP  P     W   
Sbjct: 421 IPMFA-APEVGNFARSQSSRNVTNGNLN---SASSVSRTGSTTSVPPPPPPSSNLAWTSY 480

Query: 481 ENSPTQFPRRL--TEYVRRQLFSS----ATNESGGRTSNYSQRSGSTSAQDMVLS-SGSG 540
           +NSP    RR   +E  RR L SS    ATN+  G        +   S+  +VL   G  
Sbjct: 481 QNSPHYQRRRTERSELARRSLLSSLAADATNQRSGDHPTLRSLAPPASSDGLVLQPGGDN 540

Query: 541 RRGHHLLQPRSALWMERRGDSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRR-- 600
            + H+    R+    +R+GDS +G+P+ LR LA +  G   +RL+  Q++NVL ++RR  
Sbjct: 541 SQMHNRAYSRAGPLFDRQGDSVVGIPHPLRALAAASRG--RSRLMVSQMQNVLDVMRRDA 600

Query: 601 -GESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEE 660
              +LR+EDVM+L+ S+ F  A  +DR+RDMRLDVDNMSYEELLALEERIG+V TG++EE
Sbjct: 601 NNNNLRLEDVMLLNHSVLFDGATGHDRYRDMRLDVDNMSYEELLALEERIGDVCTGVNEE 660

Query: 661 TIVARLKRTKHVNAPEPQVEEEPCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNI 707
           TI  RLK+ K+ +  +   + EPCCVCQEEY EGED+G L+CGH+FH+QCIK+WL QKN+
Sbjct: 661 TISNRLKQRKYKSNTKSPQDAEPCCVCQEEYTEGEDMGTLECGHEFHSQCIKEWLKQKNL 681

BLAST of CmaCh12G005600 vs. TAIR 10
Match: AT1G45180.1 (RING/U-box superfamily protein )

HSP 1 Score: 346.3 bits (887), Expect = 5.9e-95
Identity = 266/714 (37.25%), Postives = 378/714 (52.94%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNP-GSQPVYWNNMWNPAENRIPDYLLPTTDINTGYV 60
           MQG+R+S+GSLS+ +NFE GS+S+N    Q + W N+ N  +N + DY+    D N  + 
Sbjct: 1   MQGERASLGSLSKALNFERGSTSSNAVVDQQIRWENLHNYGDNDLQDYMSSAADTNPTFA 60

Query: 61  TTVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNN 120
            +V HE+R L R+++GE SS   + E  S  ++    W  + R            E   N
Sbjct: 61  NSVYHEKRGLQRFNIGEASSSGTKNEGASLTEQ----WKGIGR-----------FEEQRN 120

Query: 121 PTLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALC 180
             L     SP+ +Q SN + +   +NLNA +  H  + + V      ++ NG        
Sbjct: 121 DKL---ELSPLFVQPSNGSRVVRDVNLNAEYNEHLEDMNPVTVHPGHFEVNGLR------ 180

Query: 181 SSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHI 240
              S+ LL            N  RAGSS+DGRRA CKRKA++ S  Q S +G     Q  
Sbjct: 181 ---SEFLL-----------DNSVRAGSSVDGRRASCKRKALDASGGQSSSTGGFREFQRG 240

Query: 241 ESGAEPTLPVFPGRYNTGSRLTIPAPPARMNQAPVRGAGEMTSNSFSESMVAESSDSSQR 300
            S +  + P +     T + L I     R      RG       + S   + E   SS R
Sbjct: 241 VSSSWISGPTYYSPAMTANDLNISLDHGR------RGLVSSAVPNLSPPTITE---SSSR 300

Query: 301 NYRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEH-VLDLRPAPAVDNISAQG- 360
           NY + ++ +  Q++ +    ++  PG         ++PA+H  + +R   A+ N ++Q  
Sbjct: 301 NYPVWVNPTYQQETVSNFAPSLNSPG---------LIPADHQQISMRYGHALGNFASQNP 360

Query: 361 QPLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAG-ERQVVQREEVRPSNMGRNVSEHPM 420
                H+P  P S   ++WN S  +   SSSS+   +R V+ R   R  +   N  E P+
Sbjct: 361 NAPATHMP--PVSRNTFQWNTSPVAAVISSSSATPVDRNVIHRNATRQRS---NTLEIPL 420

Query: 421 FVPANELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVHP-PSGPWMPP-ENSPTQ 480
           FVPA ELRN+     + G  S N S    VAS+S   S + V P PS P + P +N+   
Sbjct: 421 FVPAPELRNV-----AHGHISRNASGARHVASSS---SRTSVQPSPSSPALTPYQNNSLH 480

Query: 481 FPRRLTEYVRRQLFSSATNESGGRTSNYSQRSGSTSAQDMVLSSGSGRRG--HHLLQPRS 540
             RRL+E  RR L SS   +     S     +   S  + VL SG       H+    R+
Sbjct: 481 NQRRLSENFRRSLLSSLVTQQRAARS----LAHPASPNEHVLQSGGDNTSQVHNRASSRA 540

Query: 541 ALWMERRGDSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESLRVEDVMILD 600
                R+G    G+ +SLR LA++  G    R+ + +IRN+L  +RR  +LR+EDVM+L+
Sbjct: 541 G---PRQGQDATGISHSLRGLASTSRG--RTRMGASEIRNILEHMRRAGNLRLEDVMLLN 600

Query: 601 QSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVARLKRTKHVNA 660
           QS+  G ADI+DR+RDMRLDVDNM+YEELL+LEERIG+V TGL+EETI  RLK+ K+ ++
Sbjct: 601 QSIMLGAADIHDRYRDMRLDVDNMTYEELLSLEERIGDVCTGLNEETISNRLKQQKYKSS 636

Query: 661 PEPQVEEEPCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPICKTTG 707
                E EPCCVCQEEYKE E+IG L+CGH FH+QCIK+WL QKN+CPICKTTG
Sbjct: 661 TRSSQEVEPCCVCQEEYKEEEEIGRLECGHDFHSQCIKEWLKQKNLCPICKTTG 636

BLAST of CmaCh12G005600 vs. TAIR 10
Match: AT4G34040.1 (RING/U-box superfamily protein )

HSP 1 Score: 294.3 bits (752), Expect = 2.7e-79
Identity = 254/727 (34.94%), Postives = 354/727 (48.69%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQG RS+ G  S  IN+  G    +  S+    NN+ NP + + P+    T      Y +
Sbjct: 1   MQGPRST-GDSSTGINYADGEPICSTNSETTS-NNILNPVDVQFPNN--TTGSGRPTYAS 60

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALSDEQKGEVGWSSVSRDADGPVTETRLCEPSNNP 120
           + SH  +N + WS GE SS    ++ L               +++G  T+ +L       
Sbjct: 61  SSSHVVQNHNWWSFGESSSRLGPSDHL---------------NSNGSKTDRQLLSDGYG- 120

Query: 121 TLGHVNFSPMIIQNSNSNALPHSLNLNASFASHGGESSRVNEGSNLYKSNGSEEGRALCS 180
                       +  +   LP    L  S +SH    S VN G ++   +G +    +  
Sbjct: 121 ----------FEEGQSGMLLPGESFLRGSSSSH--MLSHVNLGKDMDIGSGLQTSGVVIR 180

Query: 181 SASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSVTQPSLSGSSNYAQHIE 240
             +    L  G+S           GSSL G  + CKRKA+EG+ +      S       E
Sbjct: 181 HNNCETSL--GSSSQTAEERSSGPGSSLGGLGSSCKRKALEGAPSHSFPGESHGCFFQTE 240

Query: 241 SGAEPTLPVFPGRYNTGSRLTIPAP----PARMNQA-------PVRGAGEMTSNSFSESM 300
           +GA         +Y+  S L++  P    P   NQ+        + G   +T+++F  + 
Sbjct: 241 NGA---WNEGLAQYDASSSLSLSMPSQNSPNVNNQSGLPEPRFGLGGGRAVTASAFPSTR 300

Query: 301 VAESSDSSQRNYRIRISSSNAQDSFAPTGMTVRHPGASSSQLPARILPAEHVLDLRPAPA 360
             E+     R         +   SF  +G +VR       QLPA   P    LD R  P 
Sbjct: 301 STETISRPGRRLNPGQPPESVAFSFTQSGSSVR----QQQQLPA-TSPFVDPLDARAIPV 360

Query: 361 V-DNISAQGQPLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAGERQVVQREEVRPSNMG 420
              + S  GQP MIH+PAL R++  + W+ SS+SR+ S          +  E + P +  
Sbjct: 361 TGSSSSGDGQPSMIHLPALTRNIHQFAWSASSSSRANS----------MPEEGLSPWDAP 420

Query: 421 RNVSEHPMF-VPANELRNLVRNPSSRGLASSNLSIPGTVASTSRVGSSSGVH--PPSGPW 480
           R  SE P+F  PANE RN V++         N S  G      R GSSSG+H   P+  W
Sbjct: 421 RINSEQPVFTTPANETRNPVQDQFCWSFTRGNPSTSGDSPFVPRAGSSSGIHGLQPNPTW 480

Query: 481 MPPENSPTQFPRRLTEYVRRQLFSSATNESG--GRTSNYSQRSGSTSAQDMVLSSGSGRR 540
           + P N       R++E     LF S  +ES   G +        S S+ +    SGS  R
Sbjct: 481 VTPHNQ-----SRISEVAPWSLFPSIESESATHGASLPLLPTGPSVSSNEAAAPSGSSSR 540

Query: 541 GHHLLQPRSALWMERRGDSGLGLPYSLRTLATSGEGSDNNRLVSEQIRNVLGLVRRGESL 600
            H   Q RS L +ER+ D  L L +  R+LA   +G   NRL+SE IR VL  +RRGE+L
Sbjct: 541 SHRSRQRRSGLLLERQNDH-LHLRHLGRSLAADNDG--RNRLISE-IRQVLSAMRRGENL 600

Query: 601 RVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELLALEERIGNVNTGLSEETIVAR 660
           R ED M+ D  ++ GMA+++DRHRDMRLDVDNMSYEELLAL ERIG+V+TGLSEE I+  
Sbjct: 601 RFEDYMVFDPLIYQGMAEMHDRHRDMRLDVDNMSYEELLALGERIGDVSTGLSEEVILKV 660

Query: 661 LKRTKHV-NAPEPQVEEEPCCVCQEEYKEGEDIGMLDCGHKFHTQCIKQWLMQKNICPIC 710
           +K+ KH  +A     + EPCCVCQEEY EG+D+G L CGH+FHT C+KQWLM KN+CPIC
Sbjct: 661 MKQHKHTSSAAGSHQDMEPCCVCQEEYAEGDDLGTLGCGHEFHTACVKQWLMLKNLCPIC 666

BLAST of CmaCh12G005600 vs. TAIR 10
Match: AT2G15530.1 (RING/U-box superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 1.0e-70
Identity = 244/746 (32.71%), Postives = 356/746 (47.72%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQG R S+G  S  +N   G S           N M NPA+   P+   P+      Y +
Sbjct: 4   MQGPR-SIGGSSTEVNQVDGESIY---CTETSLNTMLNPADTGFPNNSTPSG--RPTYAS 63

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALS------------DEQKGEVGWSSVSRDA--DG 120
           + SH  ++ + W  GE SS    ++ ++            D     VG+ S  R    +G
Sbjct: 64  SSSHAAQDHTWWRFGESSSIPGPSDQVNSIGIKTSHQLPQDGTHHFVGYGSEGRQTGLNG 123

Query: 121 PVTETRLCEPSNNPTLGHVNFSPMIIQNSNSNALPHSLNLNASFASH--GGESSRVNEGS 180
            + +  +   S      H+   P  ++ S+SN +P  ++++    S     ++S V    
Sbjct: 124 MMVDGGVHAGS------HIRNVPSFLRGSSSNPMPQHVDMSMDMDSDNCNAQTSGVVIRH 183

Query: 181 NLYKSNGSEEGRALCSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSV 240
           N Y S+     +A   S+S P                    S   G  + CKRKA+EGS 
Sbjct: 184 NSYGSSLGSSVQAAGESSSGP-------------------ASPFGGWGSSCKRKALEGS- 243

Query: 241 TQPSLSGSSNYAQHIESGAEPTLPVFPGRYNTGSRLTIPAP-PARMNQAPVRGAGEMTSN 300
             PS   S      I             +Y   S L++  P  +  N     G  E    
Sbjct: 244 --PSHYFSGETPNRIVQTENSASHASLSQYGASSSLSLATPSQSSPNVTNHFGRTEQMFG 303

Query: 301 SFSESMVAESSDSSQRNYRI------RISSSNAQDSFAPT----GMTVRHPGASSSQLPA 360
           S     VA S+  S RN         R++    Q+S A +    G +VR  G+    LP 
Sbjct: 304 SGGGRAVAASAFHSTRNTDTLSRAGRRLNPRQPQESVAFSVSHGGTSVRPTGSLQQNLPL 363

Query: 361 RILPAEHVLDLRPAPAVD-NISAQGQPLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAG 420
              P     D+R +     + + + Q  ++H+PAL R++  Y W+ S +SR+ S+ S  G
Sbjct: 364 N-SPFVDPPDVRSSSITSGSNTGENQTNIVHLPALTRNIHQYAWDASFSSRA-SNPSGIG 423

Query: 421 ERQVVQREEVRPS-NMGRNVSEHPMFVPANELRNLVRN-----PSSRGLASSNLSIPGTV 480
               +  E + P     R+  E P+F PA ++R  V +       S G +  +L +P   
Sbjct: 424 ----MPAERLGPQWETPRSNQEQPLFAPATDMRQPVHDLWNFARGSPGSSVDSLFVP--- 483

Query: 481 ASTSRVGSSSGVH--PPSGPWMPPENSPTQFPRRLTEYVRRQLFSS--ATNESGGRTSNY 540
               R G SS +H   P+  W+PP+N+P   P R +E     LF S  + + S G     
Sbjct: 484 ----RAGPSSAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIESPSASHGGPLPL 543

Query: 541 SQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRGDSGLGLPYSLRTLATSGEGSDN 600
                S S+ ++ + S S  R H     RS L +ER+ +  L L +  R+LA  G G   
Sbjct: 544 LPAGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNEL-LHLRHIGRSLAADGNG--R 603

Query: 601 NRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELL 660
           N+++SE IR VL  +RRGE+LRVED M+ D  ++ GM D++DRHR+MRLDVDNMSYEELL
Sbjct: 604 NQIISE-IRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELL 663

Query: 661 ALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVE----EEPCCVCQEEYKEGEDIGML 705
           AL ERIG+V+TGLSEE I+  +K+ KH ++    VE     EPCC+CQEEY EG+++G L
Sbjct: 664 ALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQEEYVEGDNLGTL 698

BLAST of CmaCh12G005600 vs. TAIR 10
Match: AT2G15530.2 (RING/U-box superfamily protein )

HSP 1 Score: 265.8 bits (678), Expect = 1.0e-70
Identity = 244/746 (32.71%), Postives = 356/746 (47.72%), Query Frame = 0

Query: 1   MQGQRSSVGSLSETINFEHGSSSNNPGSQPVYWNNMWNPAENRIPDYLLPTTDINTGYVT 60
           MQG R S+G  S  +N   G S           N M NPA+   P+   P+      Y +
Sbjct: 4   MQGPR-SIGGSSTEVNQVDGESIY---CTETSLNTMLNPADTGFPNNSTPSG--RPTYAS 63

Query: 61  TVSHEQRNLSRWSLGEPSSCDMQTEALS------------DEQKGEVGWSSVSRDA--DG 120
           + SH  ++ + W  GE SS    ++ ++            D     VG+ S  R    +G
Sbjct: 64  SSSHAAQDHTWWRFGESSSIPGPSDQVNSIGIKTSHQLPQDGTHHFVGYGSEGRQTGLNG 123

Query: 121 PVTETRLCEPSNNPTLGHVNFSPMIIQNSNSNALPHSLNLNASFASH--GGESSRVNEGS 180
            + +  +   S      H+   P  ++ S+SN +P  ++++    S     ++S V    
Sbjct: 124 MMVDGGVHAGS------HIRNVPSFLRGSSSNPMPQHVDMSMDMDSDNCNAQTSGVVIRH 183

Query: 181 NLYKSNGSEEGRALCSSASDPLLLPSGNSGLPVVANDGRAGSSLDGRRAPCKRKAIEGSV 240
           N Y S+     +A   S+S P                    S   G  + CKRKA+EGS 
Sbjct: 184 NSYGSSLGSSVQAAGESSSGP-------------------ASPFGGWGSSCKRKALEGS- 243

Query: 241 TQPSLSGSSNYAQHIESGAEPTLPVFPGRYNTGSRLTIPAP-PARMNQAPVRGAGEMTSN 300
             PS   S      I             +Y   S L++  P  +  N     G  E    
Sbjct: 244 --PSHYFSGETPNRIVQTENSASHASLSQYGASSSLSLATPSQSSPNVTNHFGRTEQMFG 303

Query: 301 SFSESMVAESSDSSQRNYRI------RISSSNAQDSFAPT----GMTVRHPGASSSQLPA 360
           S     VA S+  S RN         R++    Q+S A +    G +VR  G+    LP 
Sbjct: 304 SGGGRAVAASAFHSTRNTDTLSRAGRRLNPRQPQESVAFSVSHGGTSVRPTGSLQQNLPL 363

Query: 361 RILPAEHVLDLRPAPAVD-NISAQGQPLMIHVPALPRSLQPYRWNGSSTSRSGSSSSSAG 420
              P     D+R +     + + + Q  ++H+PAL R++  Y W+ S +SR+ S+ S  G
Sbjct: 364 N-SPFVDPPDVRSSSITSGSNTGENQTNIVHLPALTRNIHQYAWDASFSSRA-SNPSGIG 423

Query: 421 ERQVVQREEVRPS-NMGRNVSEHPMFVPANELRNLVRN-----PSSRGLASSNLSIPGTV 480
               +  E + P     R+  E P+F PA ++R  V +       S G +  +L +P   
Sbjct: 424 ----MPAERLGPQWETPRSNQEQPLFAPATDMRQPVHDLWNFARGSPGSSVDSLFVP--- 483

Query: 481 ASTSRVGSSSGVH--PPSGPWMPPENSPTQFPRRLTEYVRRQLFSS--ATNESGGRTSNY 540
               R G SS +H   P+  W+PP+N+P   P R +E     LF S  + + S G     
Sbjct: 484 ----RAGPSSAIHTPQPNPTWIPPQNAPPHNPSRTSELSPWSLFPSIESPSASHGGPLPL 543

Query: 541 SQRSGSTSAQDMVLSSGSGRRGHHLLQPRSALWMERRGDSGLGLPYSLRTLATSGEGSDN 600
                S S+ ++ + S S  R H     RS L +ER+ +  L L +  R+LA  G G   
Sbjct: 544 LPAGPSVSSNEVTMPSSSNSRSHRSRHRRSGLLLERQNEL-LHLRHIGRSLAADGNG--R 603

Query: 601 NRLVSEQIRNVLGLVRRGESLRVEDVMILDQSLFFGMADIYDRHRDMRLDVDNMSYEELL 660
           N+++SE IR VL  +RRGE+LRVED M+ D  ++ GM D++DRHR+MRLDVDNMSYEELL
Sbjct: 604 NQIISE-IRQVLHAMRRGENLRVEDYMVFDPLIYQGMTDMHDRHREMRLDVDNMSYEELL 663

Query: 661 ALEERIGNVNTGLSEETIVARLKRTKHVNAPEPQVE----EEPCCVCQEEYKEGEDIGML 705
           AL ERIG+V+TGLSEE I+  +K+ KH ++    VE     EPCC+CQEEY EG+++G L
Sbjct: 664 ALGERIGDVSTGLSEEVILKAMKQHKHTSSSPSSVELHQNIEPCCICQEEYVEGDNLGTL 698

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9FMM43.2e-10138.19Probable E3 ubiquitin-protein ligase RHG1A OS=Arabidopsis thaliana OX=3702 GN=RH... [more]
O495003.8e-7834.94E3 ubiquitin-protein ligase MBR2 OS=Arabidopsis thaliana OX=3702 GN=MBR2 PE=1 SV... [more]
Q7XTV77.1e-7734.14Probable E3 ubiquitin-protein ligase HIP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Q9ZQF91.4e-6932.71E3 ubiquitin-protein ligase MBR1 OS=Arabidopsis thaliana OX=3702 GN=MBR1 PE=1 SV... [more]
Q5QLR52.2e-3351.52Probable E3 ubiquitin-protein ligase ZFP1 OS=Oryza sativa subsp. japonica OX=399... [more]
Match NameE-valueIdentityDescription
A0A6J1KSD90.0e+00100.00probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita maxima OX=366... [more]
A0A6J1KMU00.0e+0099.86probable E3 ubiquitin-protein ligase RHG1A isoform X2 OS=Cucurbita maxima OX=366... [more]
A0A6J1GIQ00.0e+0099.02probable E3 ubiquitin-protein ligase RHG1A isoform X1 OS=Cucurbita moschata OX=3... [more]
A0A6J1GHK10.0e+0098.87probable E3 ubiquitin-protein ligase RHG1A isoform X2 OS=Cucurbita moschata OX=3... [more]
A0A0A0LND70.0e+0087.66RING-type domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_2G356630 P... [more]
Match NameE-valueIdentityDescription
XP_023002037.10.0e+00100.00probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita maxima] >XP_023... [more]
XP_023002040.10.0e+0099.86probable E3 ubiquitin-protein ligase RHG1A isoform X2 [Cucurbita maxima][more]
XP_022951413.10.0e+0099.02probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita moschata] >XP_0... [more]
KAG6585677.10.0e+0098.45putative E3 ubiquitin-protein ligase RHG1A, partial [Cucurbita argyrosperma subs... [more]
XP_023536901.10.0e+0098.45probable E3 ubiquitin-protein ligase RHG1A isoform X1 [Cucurbita pepo subsp. pep... [more]
Match NameE-valueIdentityDescription
AT5G42940.12.2e-10238.19RING/U-box superfamily protein [more]
AT1G45180.15.9e-9537.25RING/U-box superfamily protein [more]
AT4G34040.12.7e-7934.94RING/U-box superfamily protein [more]
AT2G15530.11.0e-7032.71RING/U-box superfamily protein [more]
AT2G15530.21.0e-7032.71RING/U-box superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR001841Zinc finger, RING-typeSMARTSM00184ring_2coord: 662..702
e-value: 3.9E-7
score: 39.7
IPR001841Zinc finger, RING-typePFAMPF13639zf-RING_2coord: 662..703
e-value: 4.5E-11
score: 42.9
IPR001841Zinc finger, RING-typePROSITEPS50089ZF_RING_2coord: 662..703
score: 12.769585
IPR013083Zinc finger, RING/FYVE/PHD-typeGENE3D3.30.40.10Zinc/RING finger domain, C3HC4 (zinc finger)coord: 625..706
e-value: 1.2E-17
score: 65.3
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 276..327
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..29
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 489..525
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 430..457
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 373..395
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 489..517
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 152..175
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 373..416
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 89..110
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 430..475
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 242..328
NoneNo IPR availablePANTHERPTHR22937E3 UBIQUITIN-PROTEIN LIGASE RNF165coord: 1..707
NoneNo IPR availablePANTHERPTHR22937:SF139E3 UBIQUITIN-PROTEIN LIGASE MBR2-LIKE ISOFORM X1coord: 1..707
NoneNo IPR availableCDDcd16481RING-H2_TTC3coord: 661..703
e-value: 1.32971E-18
score: 77.3873
NoneNo IPR availableSUPERFAMILY57850RING/U-boxcoord: 651..705

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G005600.1CmaCh12G005600.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0016567 protein ubiquitination
cellular_component GO:0005634 nucleus
molecular_function GO:0061630 ubiquitin protein ligase activity
molecular_function GO:0008270 zinc ion binding