Homology
BLAST of CmaCh12G004730 vs. ExPASy Swiss-Prot
Match:
Q3V1L4 (Cytosolic purine 5'-nucleotidase OS=Mus musculus OX=10090 GN=Nt5c2 PE=1 SV=2)
HSP 1 Score: 229.9 bits (585), Expect = 7.2e-59
Identity = 164/498 (32.93%), Postives = 249/498 (50.00%), Query Frame = 0
Query: 92 QIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFD 151
++F NRSL M+ I GFDMDYTLA YK +ESL +E T+ +LV + Y +ELL++++D
Sbjct: 34 RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYD 93
Query: 152 WKYMVRGLVLDKRRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYA 211
+ RGLV D GN+LK+D + + V HGF + + E Y + ++ D +
Sbjct: 94 STFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFY 153
Query: 212 LIDTLFSLAEAYLFAQLVDFKDNNPDKVPED---------VDYARMYKDVRAAVDLCHRD 271
+++TLF+L E YL A LVDF N P D + Y M++DVR AVD H
Sbjct: 154 ILNTLFNLPETYLLACLVDFFTNCPRYTSCDTGFKDGDLFMSYRSMFQDVRDAVDWVHYK 213
Query: 272 GTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPD 331
G+LK+ + ++++ D + +L +++ G+ FL TNS + YT+ +M +L
Sbjct: 214 GSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGK-VFLATNSDYKYTDKIMTYLFDFPHGP 273
Query: 332 GDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTS 391
+ W YFD+++ + KP FF E L VD K+G L PL S
Sbjct: 274 KPGSSHRPWQSYFDLILVDARKPLFFGEG--TVLRQVDTKTGKLKIGTYTGPLQHGIVYS 333
Query: 392 EASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYVGDHIYGDILRSKKALGWRTMLV 451
SS T D L +LY+GDHI+GDIL+SKK GWRT LV
Sbjct: 334 GGSSDTICD-----------------LLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLV 393
Query: 452 VPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHLKWSLKFEDVKADEKERLSAELH 511
+PEL +E+ + + ++LQ L D +++ K D ++E+ +S
Sbjct: 394 IPELAQELHVWTDKSSLFEELQSL----DIFLAELY------KHLDSSSNERPDIS---- 453
Query: 512 QLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLS 571
S Q R++ D +G+ G L ++G + + FA QV R+A LY++ NL
Sbjct: 454 ---SIQRRIKKVTHDM-----DMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLL 487
Query: 572 LYSPAKYYRPNEDFMPHE 581
Y + +R MPHE
Sbjct: 514 YYPFSYLFRAAHVLMPHE 487
BLAST of CmaCh12G004730 vs. ExPASy Swiss-Prot
Match:
O46411 (Cytosolic purine 5'-nucleotidase OS=Bos taurus OX=9913 GN=NT5C2 PE=1 SV=1)
HSP 1 Score: 228.8 bits (582), Expect = 1.6e-58
Identity = 163/498 (32.73%), Postives = 249/498 (50.00%), Query Frame = 0
Query: 92 QIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFD 151
++F NRSL M+ I GFDMDYTLA YK +ESL +E T+ +LV + Y +ELL++++D
Sbjct: 34 RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYD 93
Query: 152 WKYMVRGLVLDKRRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYA 211
+ RGLV D GN+LK+D + + V HGF + + E Y + ++ D +
Sbjct: 94 STFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFY 153
Query: 212 LIDTLFSLAEAYLFAQLVDFKDNNPDKVPED---------VDYARMYKDVRAAVDLCHRD 271
+++TLF+L E YL A LVDF N P + + Y M++DVR AVD H
Sbjct: 154 ILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYK 213
Query: 272 GTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPD 331
G+LK+ + ++++ D + +L +++ G+ FL TNS + YT+ +M +L
Sbjct: 214 GSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGK-VFLATNSDYKYTDKIMTYLFDFPHGP 273
Query: 332 GDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTS 391
+ W YFD+++ + KP FF E L VD K+G L PL S
Sbjct: 274 KPGSSHRPWQSYFDLILVDARKPLFFGEG--TVLRQVDTKTGKLKIGTYTGPLQHGIVYS 333
Query: 392 EASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYVGDHIYGDILRSKKALGWRTMLV 451
SS T D L +LY+GDHI+GDIL+SKK GWRT LV
Sbjct: 334 GGSSDTVCD-----------------LLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLV 393
Query: 452 VPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHLKWSLKFEDVKADEKERLSAELH 511
+PEL +E+ + + ++LQ L D +++ K D ++E+ +S
Sbjct: 394 IPELAQELHVWTDKSSLFEELQSL----DIFLAELY------KHLDSSSNERPDIS---- 453
Query: 512 QLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLS 571
S Q R++ D +G+ G L ++G + + FA QV R+A LY++ NL
Sbjct: 454 ---SIQRRIKKVTHDM-----DMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLL 487
Query: 572 LYSPAKYYRPNEDFMPHE 581
Y + +R MPHE
Sbjct: 514 YYPFSYLFRAAHVLMPHE 487
BLAST of CmaCh12G004730 vs. ExPASy Swiss-Prot
Match:
P49902 (Cytosolic purine 5'-nucleotidase OS=Homo sapiens OX=9606 GN=NT5C2 PE=1 SV=1)
HSP 1 Score: 228.4 bits (581), Expect = 2.1e-58
Identity = 163/498 (32.73%), Postives = 249/498 (50.00%), Query Frame = 0
Query: 92 QIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFD 151
++F NRSL M+ I GFDMDYTLA YK +ESL +E T+ +LV + Y +ELL++++D
Sbjct: 34 RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYD 93
Query: 152 WKYMVRGLVLDKRRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYA 211
+ RGLV D GN+LK+D + + V HGF + + E Y + ++ D +
Sbjct: 94 STFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFY 153
Query: 212 LIDTLFSLAEAYLFAQLVDFKDNNPDKVPED---------VDYARMYKDVRAAVDLCHRD 271
+++TLF+L E YL A LVDF N P + + Y M++DVR AVD H
Sbjct: 154 ILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYK 213
Query: 272 GTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPD 331
G+LK+ + ++++ D + +L +++ G+ FL TNS + YT+ +M +L
Sbjct: 214 GSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGK-VFLATNSDYKYTDKIMTYLFDFPHGP 273
Query: 332 GDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTS 391
+ W YFD+++ + KP FF E L VD K+G L PL S
Sbjct: 274 KPGSSHRPWQSYFDLILVDARKPLFFGEG--TVLRQVDTKTGKLKIGTYTGPLQHGIVYS 333
Query: 392 EASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYVGDHIYGDILRSKKALGWRTMLV 451
SS T D L +LY+GDHI+GDIL+SKK GWRT LV
Sbjct: 334 GGSSDTICD-----------------LLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLV 393
Query: 452 VPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHLKWSLKFEDVKADEKERLSAELH 511
+PEL +E+ + + ++LQ L D +++ K D ++E+ +S
Sbjct: 394 IPELAQELHVWTDKSSLFEELQSL----DIFLAELY------KHLDSSSNERPDIS---- 453
Query: 512 QLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLS 571
S Q R++ D +G+ G L ++G + + FA QV R+A LY++ NL
Sbjct: 454 ---SIQRRIKKVTHDM-----DMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLL 487
Query: 572 LYSPAKYYRPNEDFMPHE 581
Y + +R MPHE
Sbjct: 514 YYPFSYLFRAAHVLMPHE 487
BLAST of CmaCh12G004730 vs. ExPASy Swiss-Prot
Match:
Q5RA22 (Cytosolic purine 5'-nucleotidase OS=Pongo abelii OX=9601 GN=NT5C2 PE=2 SV=1)
HSP 1 Score: 228.4 bits (581), Expect = 2.1e-58
Identity = 163/498 (32.73%), Postives = 249/498 (50.00%), Query Frame = 0
Query: 92 QIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFD 151
++F NRSL M+ I GFDMDYTLA YK +ESL +E T+ +LV + Y +ELL++++D
Sbjct: 34 RVFVNRSLAMEKIKCFGFDMDYTLAVYKSPEYESLGFELTVERLV-SIGYPQELLSFAYD 93
Query: 152 WKYMVRGLVLDKRRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYA 211
+ RGLV D GN+LK+D + + V HGF + + E Y + ++ D +
Sbjct: 94 STFPTRGLVFDTLYGNLLKVDAYGNLLVCAHGFNFIRGPETREQYPNKFIQRD-DTERFY 153
Query: 212 LIDTLFSLAEAYLFAQLVDFKDNNPDKVPED---------VDYARMYKDVRAAVDLCHRD 271
+++TLF+L E YL A LVDF N P + + Y M++DVR AVD H
Sbjct: 154 ILNTLFNLPETYLLACLVDFFTNCPRYTSCETGFKDGDLFMSYRSMFQDVRDAVDWVHYK 213
Query: 272 GTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPD 331
G+LK+ + ++++ D + +L +++ G+ FL TNS + YT+ +M +L
Sbjct: 214 GSLKEKTVENLEKYVVKDGKLPLLLSRMKEVGK-VFLATNSDYKYTDKIMTYLFDFPHGP 273
Query: 332 GDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTS 391
+ W YFD+++ + KP FF E L VD K+G L PL S
Sbjct: 274 KPGSSHRPWQSYFDLILVDARKPLFFGEG--TVLRQVDTKTGKLKIGTYTGPLQHGIVYS 333
Query: 392 EASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYVGDHIYGDILRSKKALGWRTMLV 451
SS T D L +LY+GDHI+GDIL+SKK GWRT LV
Sbjct: 334 GGSSDTICD-----------------LLGAKGKDILYIGDHIFGDILKSKKRQGWRTFLV 393
Query: 452 VPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHLKWSLKFEDVKADEKERLSAELH 511
+PEL +E+ + + ++LQ L D +++ K D ++E+ +S
Sbjct: 394 IPELAQELHVWTDKSSLFEELQSL----DIFLAELY------KHLDSSSNERPDIS---- 453
Query: 512 QLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLS 571
S Q R++ D +G+ G L ++G + + FA QV R+A LY++ NL
Sbjct: 454 ---SIQRRIKKVTHDM-----DMCYGMMGSLFRSGSRQTLFASQVMRYADLYAASFINLL 487
Query: 572 LYSPAKYYRPNEDFMPHE 581
Y + +R MPHE
Sbjct: 514 YYPFSYLFRAAHVLMPHE 487
BLAST of CmaCh12G004730 vs. ExPASy Swiss-Prot
Match:
Q54XC1 (Cytosolic purine 5'-nucleotidase OS=Dictyostelium discoideum OX=44689 GN=nt5c2 PE=3 SV=1)
HSP 1 Score: 226.1 bits (575), Expect = 1.0e-57
Identity = 167/526 (31.75%), Postives = 256/526 (48.67%), Query Frame = 0
Query: 91 KQIFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSF 150
+++F NR + + I GFDMDYTLA Y FE LAY+ + KL+ D+ Y + + +
Sbjct: 103 RRVFVNRDIKLDRIEFFGFDMDYTLAVYNSPDFEELAYDMVLDKLI-DIGYPKSIRKLKY 162
Query: 151 DWKYMVRGLVLDKRRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGSTLV-RDSFDEPD 210
D + RGL LD+ GN+LK+D + + HG LSK+ E Y S V D
Sbjct: 163 DPNFPTRGLFLDRELGNLLKIDSFGNIIICVHGRTTLSKNRTAEFYPSMRVSSDEIARNR 222
Query: 211 YALIDTLFSLAEAYLFAQLVDFKD------------------NNP--------------- 270
+ L++TLF+L EA L+A LVD + N+P
Sbjct: 223 FYLLNTLFTLPEACLYADLVDHLERESGLRLTEEVADEQQQMNSPPLSSLGSESVRIDES 282
Query: 271 DKVPE-DVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGR 330
+ PE D+ ++ +++DVR A DL H DG+LK V D R+I + LR +
Sbjct: 283 NHQPEGDLSFSNLFQDVRTACDLVHNDGSLKTRVLDDLPRYIRKTPDMPVFFDRLRQNKN 342
Query: 331 STFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRAN 390
FL+TNS + YTN VM+++ P+ W YFDV+I G+ KP FF E
Sbjct: 343 KVFLLTNSEFYYTNKVMSYMMNGYNPNYQ-----SWRDYFDVIIVGADKPRFFSEG--TT 402
Query: 391 LFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSS 450
+ VD ++G L T+ Q KV+ GG++ KL + S
Sbjct: 403 IREVDTETGNLRITNVKDRFEQ----------------GKVYHGGSLSLFQKLTGAK-GS 462
Query: 451 QVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVED 510
+VLY+GDHI+ DI++SKK GWR +LVVPEL+ E+E++ + + T L L + +
Sbjct: 463 RVLYIGDHIFADIIKSKKTHGWRNLLVVPELQHELEVMNQQKDTTIHLMNL----EFIRA 522
Query: 511 KIHHLKWSLKFEDVKADEKERLSAELHQ-LESEQERVRLSHQDALRDCHRQFHGVWGQLM 570
+I+ L E + E LHQ L++ +++ + ++ +G L
Sbjct: 523 EIYR---GLDSESTTPPDIE----VLHQHLKNTNDKLNFA-----------YNKYFGSLF 581
Query: 571 KTGYQNSRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHE 581
K G +++ F+ QV+R+A LY+S NL Y ++ N +PHE
Sbjct: 583 KNGSKSTFFSMQVQRYADLYTSDYLNLLNYPLFYHFCANSLPLPHE 581
BLAST of CmaCh12G004730 vs. ExPASy TrEMBL
Match:
A0A6J1KNI6 (5'-nucleotidase domain-containing protein 4-like isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111496172 PE=3 SV=1)
HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0
Query: 1 MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTRRLNRLRAIGAMGENLGTVMKDK 60
MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTRRLNRLRAIGAMGENLGTVMKDK
Sbjct: 1 MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTRRLNRLRAIGAMGENLGTVMKDK 60
Query: 61 RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP
Sbjct: 61 RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
Query: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA
Sbjct: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
Query: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP
Sbjct: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
Query: 241 EDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLV 300
EDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLV
Sbjct: 241 EDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLV 300
Query: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVD 360
TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVD
Sbjct: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVD 360
Query: 361 RKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
RKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV
Sbjct: 361 RKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
Query: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHL 480
GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHL
Sbjct: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHL 480
Query: 481 KWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN 540
KWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN
Sbjct: 481 KWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN 540
Query: 541 SRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 587
SRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL
Sbjct: 541 SRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 586
BLAST of CmaCh12G004730 vs. ExPASy TrEMBL
Match:
A0A6J1GIA5 (5'-nucleotidase domain-containing protein 4-like isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454408 PE=3 SV=1)
HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 568/586 (96.93%), Postives = 578/586 (98.63%), Query Frame = 0
Query: 1 MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTRRLNRLRAIGAMGENLGTVMKDK 60
MRIPR+QLSFCLSLFSLEFSYPVSPR+FLGVPKGIHT RLNR AIGAMGENLG VMKDK
Sbjct: 1 MRIPRRQLSFCLSLFSLEFSYPVSPRHFLGVPKGIHTLRLNRSCAIGAMGENLGAVMKDK 60
Query: 61 RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
R HSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP
Sbjct: 61 RPHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
Query: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA
Sbjct: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
Query: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP
Sbjct: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
Query: 241 EDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLV 300
EDVDYARMYKDVRAAVD+CHRDGTLKQMVA DPKRFIN+DTSIVPMLKMLRDSGRSTFLV
Sbjct: 241 EDVDYARMYKDVRAAVDMCHRDGTLKQMVAKDPKRFINVDTSIVPMLKMLRDSGRSTFLV 300
Query: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVD 360
TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDV+ITGSAKPGFFHEDNRANLFGV+
Sbjct: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVIITGSAKPGFFHEDNRANLFGVE 360
Query: 361 RKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
KSGMLLNTDNGSPLPQVGNTSE SSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV
Sbjct: 361 PKSGMLLNTDNGSPLPQVGNTSEMSSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
Query: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHL 480
GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDA+EDKIHHL
Sbjct: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAIEDKIHHL 480
Query: 481 KWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN 540
KWSLKFEDV+ADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN
Sbjct: 481 KWSLKFEDVEADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN 540
Query: 541 SRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 587
SRFAHQVERFACLYSSQV+NLSLYSPAKYYRP+EDFMPHEFGIIPL
Sbjct: 541 SRFAHQVERFACLYSSQVTNLSLYSPAKYYRPSEDFMPHEFGIIPL 586
BLAST of CmaCh12G004730 vs. ExPASy TrEMBL
Match:
A0A6J1KKW6 (5'-nucleotidase domain-containing protein 4-like isoform X2 OS=Cucurbita maxima OX=3661 GN=LOC111496172 PE=3 SV=1)
HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0
Query: 49 MGENLGTVMKDKRTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVG 108
MGENLGTVMKDKRTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVG
Sbjct: 1 MGENLGTVMKDKRTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVG 60
Query: 109 FDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNI 168
FDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNI
Sbjct: 61 FDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNI 120
Query: 169 LKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQL 228
LKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQL
Sbjct: 121 LKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQL 180
Query: 229 VDFKDNNPDKVPEDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLK 288
VDFKDNNPDKVPEDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLK
Sbjct: 181 VDFKDNNPDKVPEDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLK 240
Query: 289 MLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFF 348
MLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFF
Sbjct: 241 MLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFF 300
Query: 349 HEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKL 408
HEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKL
Sbjct: 301 HEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKL 360
Query: 409 LQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN 468
LQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN
Sbjct: 361 LQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN 420
Query: 469 DRDAVEDKIHHLKWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHG 528
DRDAVEDKIHHLKWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHG
Sbjct: 421 DRDAVEDKIHHLKWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHG 480
Query: 529 VWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 587
VWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL
Sbjct: 481 VWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 538
BLAST of CmaCh12G004730 vs. ExPASy TrEMBL
Match:
A0A6J1GIC9 (5'-nucleotidase domain-containing protein 4-like isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454408 PE=3 SV=1)
HSP 1 Score: 1079.3 bits (2790), Expect = 0.0e+00
Identity = 525/538 (97.58%), Postives = 533/538 (99.07%), Query Frame = 0
Query: 49 MGENLGTVMKDKRTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVG 108
MGENLG VMKDKR HSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVG
Sbjct: 1 MGENLGAVMKDKRPHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVG 60
Query: 109 FDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNI 168
FDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNI
Sbjct: 61 FDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNI 120
Query: 169 LKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQL 228
LKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQL
Sbjct: 121 LKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQL 180
Query: 229 VDFKDNNPDKVPEDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLK 288
VDFKDNNPDKVPEDVDYARMYKDVRAAVD+CHRDGTLKQMVA DPKRFIN+DTSIVPMLK
Sbjct: 181 VDFKDNNPDKVPEDVDYARMYKDVRAAVDMCHRDGTLKQMVAKDPKRFINVDTSIVPMLK 240
Query: 289 MLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFF 348
MLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDV+ITGSAKPGFF
Sbjct: 241 MLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVIITGSAKPGFF 300
Query: 349 HEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKL 408
HEDNRANLFGV+ KSGMLLNTDNGSPLPQVGNTSE SSPTKVDKSCKVFQGGNVMHLHKL
Sbjct: 301 HEDNRANLFGVEPKSGMLLNTDNGSPLPQVGNTSEMSSPTKVDKSCKVFQGGNVMHLHKL 360
Query: 409 LQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN 468
LQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN
Sbjct: 361 LQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN 420
Query: 469 DRDAVEDKIHHLKWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHG 528
DRDA+EDKIHHLKWSLKFEDV+ADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHG
Sbjct: 421 DRDAIEDKIHHLKWSLKFEDVEADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHG 480
Query: 529 VWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 587
VWGQLMKTGYQNSRFAHQVERFACLYSSQV+NLSLYSPAKYYRP+EDFMPHEFGIIPL
Sbjct: 481 VWGQLMKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPAKYYRPSEDFMPHEFGIIPL 538
BLAST of CmaCh12G004730 vs. ExPASy TrEMBL
Match:
A0A1S3BBU8 (5'-nucleotidase domain-containing protein 4 isoform X1 OS=Cucumis melo OX=3656 GN=LOC103487969 PE=3 SV=1)
HSP 1 Score: 1074.7 bits (2778), Expect = 2.2e-310
Identity = 521/593 (87.86%), Postives = 556/593 (93.76%), Query Frame = 0
Query: 1 MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGV-------PKGIHTRRLNRLRAIGAMGENL 60
MRIPR+Q+SFCLSLFSLEF+YP+S +FLG+ PK IHT LN+LR I AMGENL
Sbjct: 1 MRIPRRQVSFCLSLFSLEFTYPLSSPSFLGIPNSFIRKPKRIHTLGLNQLRPIDAMGENL 60
Query: 61 GTVMKDKRTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDY 120
GTV+K+KRTHSLDVTH+ED Q+PCIWSS EC IDIGKQIFCNRSLNMKNIVAVGFDMDY
Sbjct: 61 GTVLKEKRTHSLDVTHTEDGQEPCIWSSPEECKIDIGKQIFCNRSLNMKNIVAVGFDMDY 120
Query: 121 TLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDR 180
TLAQYKPETFESLAYEGTIRKLVYDL YSEELLNWSFDWKYMVRGLVLDK+RGNILKMDR
Sbjct: 121 TLAQYKPETFESLAYEGTIRKLVYDLGYSEELLNWSFDWKYMVRGLVLDKKRGNILKMDR 180
Query: 181 HKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKD 240
HKYVKVAYHGFKE+SKDDKVETYG+TLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKD
Sbjct: 181 HKYVKVAYHGFKEMSKDDKVETYGNTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKD 240
Query: 241 NNPDKVPEDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDS 300
NNPDKVPED+DYARMYKDVRAAVDLCHRDGTLKQMVA DP+R+IN+DTSI+PMLKMLRDS
Sbjct: 241 NNPDKVPEDIDYARMYKDVRAAVDLCHRDGTLKQMVAKDPERYINVDTSILPMLKMLRDS 300
Query: 301 GRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNR 360
GRSTFLVTNSLWDYTNVVMNFLC SSA DGDQK N DWLQYFDVVITGSAKPGFFH+DNR
Sbjct: 301 GRSTFLVTNSLWDYTNVVMNFLCNSSASDGDQKRNFDWLQYFDVVITGSAKPGFFHDDNR 360
Query: 361 ANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIES 420
ANLF V+ KSGMLLNTDNGSP+PQVG S A SPTK+ KSC+VFQGGNV HLHKLL IES
Sbjct: 361 ANLFEVEAKSGMLLNTDNGSPMPQVGTASMALSPTKLSKSCRVFQGGNVRHLHKLLSIES 420
Query: 421 SSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAV 480
SSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN+RDA+
Sbjct: 421 SSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNERDAI 480
Query: 481 EDKIHHLKWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQL 540
ED+IHHLKWSLKFED++ADEK++LS LHQLE E+ERVRLSHQDALR+CHRQFHGVWGQL
Sbjct: 481 EDQIHHLKWSLKFEDIEADEKDKLSTGLHQLEGERERVRLSHQDALRECHRQFHGVWGQL 540
Query: 541 MKTGYQNSRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 587
MKTGYQNSRFAHQVERFACLYSSQV+NLSLYSP KYYRP+EDFMPHEFGIIPL
Sbjct: 541 MKTGYQNSRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEFGIIPL 593
BLAST of CmaCh12G004730 vs. NCBI nr
Match:
XP_023002285.1 (5'-nucleotidase domain-containing protein 4-like isoform X1 [Cucurbita maxima])
HSP 1 Score: 1196.0 bits (3093), Expect = 0.0e+00
Identity = 586/586 (100.00%), Postives = 586/586 (100.00%), Query Frame = 0
Query: 1 MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTRRLNRLRAIGAMGENLGTVMKDK 60
MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTRRLNRLRAIGAMGENLGTVMKDK
Sbjct: 1 MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTRRLNRLRAIGAMGENLGTVMKDK 60
Query: 61 RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP
Sbjct: 61 RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
Query: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA
Sbjct: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
Query: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP
Sbjct: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
Query: 241 EDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLV 300
EDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLV
Sbjct: 241 EDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLV 300
Query: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVD 360
TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVD
Sbjct: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVD 360
Query: 361 RKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
RKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV
Sbjct: 361 RKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
Query: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHL 480
GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHL
Sbjct: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHL 480
Query: 481 KWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN 540
KWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN
Sbjct: 481 KWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN 540
Query: 541 SRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 587
SRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL
Sbjct: 541 SRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 586
BLAST of CmaCh12G004730 vs. NCBI nr
Match:
XP_023537165.1 (5'-nucleotidase domain-containing protein 4-like isoform X1 [Cucurbita pepo subsp. pepo])
HSP 1 Score: 1172.5 bits (3032), Expect = 0.0e+00
Identity = 572/586 (97.61%), Postives = 581/586 (99.15%), Query Frame = 0
Query: 1 MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTRRLNRLRAIGAMGENLGTVMKDK 60
MRIPR+QLSFCLSLFSLEFSYPVSPRNFLGVPKGIHT RLNR AIGAMGENLGTVMKDK
Sbjct: 1 MRIPRRQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTLRLNRSCAIGAMGENLGTVMKDK 60
Query: 61 RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP
Sbjct: 61 RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
Query: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA
Sbjct: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
Query: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP
Sbjct: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
Query: 241 EDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLV 300
EDVDYARMYKDVRAAVD+CHRDGTLKQMVA DPKRFINMDTSIVPMLKMLRDSGRSTFLV
Sbjct: 241 EDVDYARMYKDVRAAVDMCHRDGTLKQMVAKDPKRFINMDTSIVPMLKMLRDSGRSTFLV 300
Query: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVD 360
TNSLWDYTNVVMNFLCTSSAP+GDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGV+
Sbjct: 301 TNSLWDYTNVVMNFLCTSSAPEGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVE 360
Query: 361 RKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
KSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV
Sbjct: 361 PKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
Query: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHL 480
GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDA+EDKIHHL
Sbjct: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAIEDKIHHL 480
Query: 481 KWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN 540
KWSLKFED+KADEKERLSAELHQLESEQERVRLSHQDALR+CHRQFHGVWGQLMKTGYQN
Sbjct: 481 KWSLKFEDIKADEKERLSAELHQLESEQERVRLSHQDALRECHRQFHGVWGQLMKTGYQN 540
Query: 541 SRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 587
SRFAHQVERFACLYSSQV+NLSLYSPAKYYRP+EDFMPHEFGIIPL
Sbjct: 541 SRFAHQVERFACLYSSQVTNLSLYSPAKYYRPSEDFMPHEFGIIPL 586
BLAST of CmaCh12G004730 vs. NCBI nr
Match:
XP_022951662.1 (5'-nucleotidase domain-containing protein 4-like isoform X1 [Cucurbita moschata])
HSP 1 Score: 1164.4 bits (3011), Expect = 0.0e+00
Identity = 568/586 (96.93%), Postives = 578/586 (98.63%), Query Frame = 0
Query: 1 MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTRRLNRLRAIGAMGENLGTVMKDK 60
MRIPR+QLSFCLSLFSLEFSYPVSPR+FLGVPKGIHT RLNR AIGAMGENLG VMKDK
Sbjct: 1 MRIPRRQLSFCLSLFSLEFSYPVSPRHFLGVPKGIHTLRLNRSCAIGAMGENLGAVMKDK 60
Query: 61 RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
R HSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP
Sbjct: 61 RPHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
Query: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA
Sbjct: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
Query: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP
Sbjct: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
Query: 241 EDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLV 300
EDVDYARMYKDVRAAVD+CHRDGTLKQMVA DPKRFIN+DTSIVPMLKMLRDSGRSTFLV
Sbjct: 241 EDVDYARMYKDVRAAVDMCHRDGTLKQMVAKDPKRFINVDTSIVPMLKMLRDSGRSTFLV 300
Query: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVD 360
TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDV+ITGSAKPGFFHEDNRANLFGV+
Sbjct: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVIITGSAKPGFFHEDNRANLFGVE 360
Query: 361 RKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
KSGMLLNTDNGSPLPQVGNTSE SSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV
Sbjct: 361 PKSGMLLNTDNGSPLPQVGNTSEMSSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
Query: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHL 480
GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDA+EDKIHHL
Sbjct: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAIEDKIHHL 480
Query: 481 KWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN 540
KWSLKFEDV+ADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN
Sbjct: 481 KWSLKFEDVEADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN 540
Query: 541 SRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 587
SRFAHQVERFACLYSSQV+NLSLYSPAKYYRP+EDFMPHEFGIIPL
Sbjct: 541 SRFAHQVERFACLYSSQVTNLSLYSPAKYYRPSEDFMPHEFGIIPL 586
BLAST of CmaCh12G004730 vs. NCBI nr
Match:
KAG6585600.1 (Cytosolic purine 5'-nucleotidase, partial [Cucurbita argyrosperma subsp. sororia])
HSP 1 Score: 1135.6 bits (2936), Expect = 0.0e+00
Identity = 558/581 (96.04%), Postives = 568/581 (97.76%), Query Frame = 0
Query: 1 MRIPRKQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTRRLNRLRAIGAMGENLGTVMKDK 60
MRIPR+QLSFCLSLFSLEFSYPVSPRNFLGVPKGIHT RLNR AIGAMGENLGTVMKDK
Sbjct: 1 MRIPRRQLSFCLSLFSLEFSYPVSPRNFLGVPKGIHTLRLNRSCAIGAMGENLGTVMKDK 60
Query: 61 RTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
RTHSL DVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP
Sbjct: 61 RTHSL------DVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVGFDMDYTLAQYKP 120
Query: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA
Sbjct: 121 ETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNILKMDRHKYVKVA 180
Query: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP
Sbjct: 181 YHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQLVDFKDNNPDKVP 240
Query: 241 EDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLKMLRDSGRSTFLV 300
EDVDYARMYKDVRAAVD+CHRDGTLKQMVA DPKRFIN+DTSIVP+LKMLRDSGRSTFLV
Sbjct: 241 EDVDYARMYKDVRAAVDMCHRDGTLKQMVAKDPKRFINVDTSIVPLLKMLRDSGRSTFLV 300
Query: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVD 360
TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGV+
Sbjct: 301 TNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFFHEDNRANLFGVE 360
Query: 361 RKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
KSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV
Sbjct: 361 PKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKLLQIESSSQVLYV 420
Query: 421 GDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRNDRDAVEDKIHHL 480
GDHIYGDILRSKKALGWRTMLVVPEL REVELLWELRKTRKQLQLLRNDRDA+EDKIHHL
Sbjct: 421 GDHIYGDILRSKKALGWRTMLVVPELGREVELLWELRKTRKQLQLLRNDRDAIEDKIHHL 480
Query: 481 KWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTGYQN 540
KWSLKFEDV+ADEKERLSAELHQLESEQERVRLSHQ+ALRDCHRQFHGVWGQLMKTGYQN
Sbjct: 481 KWSLKFEDVEADEKERLSAELHQLESEQERVRLSHQNALRDCHRQFHGVWGQLMKTGYQN 540
Query: 541 SRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEF 582
SRFAHQVERFACLYSSQV+NLSLYSP KYYRP+EDFMPHEF
Sbjct: 541 SRFAHQVERFACLYSSQVTNLSLYSPDKYYRPSEDFMPHEF 575
BLAST of CmaCh12G004730 vs. NCBI nr
Match:
XP_023002286.1 (5'-nucleotidase domain-containing protein 4-like isoform X2 [Cucurbita maxima])
HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 538/538 (100.00%), Postives = 538/538 (100.00%), Query Frame = 0
Query: 49 MGENLGTVMKDKRTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVG 108
MGENLGTVMKDKRTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVG
Sbjct: 1 MGENLGTVMKDKRTHSLDVTHSEDVQQPCIWSSSVECNIDIGKQIFCNRSLNMKNIVAVG 60
Query: 109 FDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNI 168
FDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNI
Sbjct: 61 FDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNI 120
Query: 169 LKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQL 228
LKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQL
Sbjct: 121 LKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQL 180
Query: 229 VDFKDNNPDKVPEDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLK 288
VDFKDNNPDKVPEDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLK
Sbjct: 181 VDFKDNNPDKVPEDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLK 240
Query: 289 MLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFF 348
MLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFF
Sbjct: 241 MLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFF 300
Query: 349 HEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKL 408
HEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKL
Sbjct: 301 HEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKL 360
Query: 409 LQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN 468
LQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN
Sbjct: 361 LQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN 420
Query: 469 DRDAVEDKIHHLKWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHG 528
DRDAVEDKIHHLKWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHG
Sbjct: 421 DRDAVEDKIHHLKWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHG 480
Query: 529 VWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 587
VWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL
Sbjct: 481 VWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 538
BLAST of CmaCh12G004730 vs. TAIR 10
Match:
AT1G75210.1 (HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase )
HSP 1 Score: 836.6 bits (2160), Expect = 1.2e-242
Identity = 418/598 (69.90%), Postives = 474/598 (79.26%), Query Frame = 0
Query: 1 MRIPRKQLSFCLSLFSLEFS-----------YPVSPRNFLGVPKGIHTRRLNRLRAIGAM 60
MR+ ++QLS LSL FS YP R FL HT ++L +M
Sbjct: 51 MRLSKRQLSLPLSLCFSTFSPRSRFHHFPGKYPAPRREFL------HTTGTSQLCFKSSM 110
Query: 61 GENLGTVMKDKRTHSLDVTHSEDVQQPCIWSS-SVECNIDIGKQIFCNRSLNMKNIVAVG 120
+ + + + V + P WSS IDI QIFCNRSLNMKNI+AVG
Sbjct: 111 EDQKPSALSENVLQFHTVGSRDGCDVPHEWSSPEGGKKIDISNQIFCNRSLNMKNIIAVG 170
Query: 121 FDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDWKYMVRGLVLDKRRGNI 180
FDMDYTLAQYK ETFESLAY+GT+RKLVYDL Y ELL W+FDW YMVRGLVLDK+RGNI
Sbjct: 171 FDMDYTLAQYKSETFESLAYDGTVRKLVYDLGYPNELLEWTFDWNYMVRGLVLDKKRGNI 230
Query: 181 LKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYALIDTLFSLAEAYLFAQL 240
LKMDRHKYVKVAYHGF+ELSK+DKVE YGS+LVRDSFDEPDYALIDTLFSLAEAYLFAQL
Sbjct: 231 LKMDRHKYVKVAYHGFRELSKEDKVEIYGSSLVRDSFDEPDYALIDTLFSLAEAYLFAQL 290
Query: 241 VDFKDNNPDKVPEDVDYARMYKDVRAAVDLCHRDGTLKQMVATDPKRFINMDTSIVPMLK 300
VDFKDNNP+KVP+DVDYARMYKDVRAAVDLCHRDGTLKQMVA +P R+IN DT+IVP++K
Sbjct: 291 VDFKDNNPEKVPKDVDYARMYKDVRAAVDLCHRDGTLKQMVAKEPNRYINEDTTIVPLIK 350
Query: 301 MLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDWLQYFDVVITGSAKPGFF 360
M+RDSGRSTFLVTNSLWDYTN+VMNFLC G CN DWLQYFDVVITGSAKPGFF
Sbjct: 351 MIRDSGRSTFLVTNSLWDYTNIVMNFLCGGRTVHGPHTCNFDWLQYFDVVITGSAKPGFF 410
Query: 361 HEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVDKSCKVFQGGNVMHLHKL 420
HED+RANLF V+ +SGML+NTDNG+P+ QVG+ S DK C+VFQGGNV HLH L
Sbjct: 411 HEDSRANLFEVEPQSGMLINTDNGTPMAQVGDPSPKILLKSKDKGCRVFQGGNVGHLHSL 470
Query: 421 LQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVELLWELRKTRKQLQLLRN 480
L IESSSQVLYVGDHIYGDILRSKK LGWRTMLVVPELE+EVELLWELR RK L L+RN
Sbjct: 471 LSIESSSQVLYVGDHIYGDILRSKKILGWRTMLVVPELEKEVELLWELRNMRKDLILMRN 530
Query: 481 DRDAVEDKIHHLKWSLKFEDVKADEKERLSAELHQLESEQERVRLSHQDALRDCHRQFHG 540
+RD+VEDKIHHL WSLKFED+ + K + + L LES++++VR SHQ A R+CH++FH
Sbjct: 531 ERDSVEDKIHHLNWSLKFEDINENNKHEMLSALKDLESKRDKVRQSHQQAQRECHQKFHK 590
Query: 541 VWGQLMKTGYQNSRFAHQVERFACLYSSQVSNLSLYSPAKYYRPNEDFMPHEFGIIPL 587
VWGQLMKTGYQ+SRFAHQVERFACLY+SQVSNL LYSP KYYRP+EDFM HEF ++PL
Sbjct: 591 VWGQLMKTGYQSSRFAHQVERFACLYTSQVSNLRLYSPDKYYRPSEDFMSHEFHLLPL 642
BLAST of CmaCh12G004730 vs. TAIR 10
Match:
AT5G48960.1 (HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase )
HSP 1 Score: 218.8 bits (556), Expect = 1.2e-56
Identity = 154/500 (30.80%), Postives = 249/500 (49.80%), Query Frame = 0
Query: 93 IFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDW 152
IFC+R+LN+++I A+G+DMDYTL Y +E AY+ + L + + + L +FD
Sbjct: 165 IFCSRTLNLRSISAIGYDMDYTLMHYNVMAWEGKAYDYCMENL-KSMGFPVDGL--AFDP 224
Query: 153 KYMVRGLVLDKRRGNILKMDRHKYVKVAYHGFKELSKDDKVETYGSTLVRDSFDEPDYAL 212
+ ++RGL++DK +GN++K DR YVK A HG K LS E YG LV D ++ +
Sbjct: 225 ELVIRGLMIDKEKGNLVKADRFGYVKRAMHGTKMLSNKAVSEIYGRELV-DLRNQSRWEF 284
Query: 213 IDTLFSLAEAYLFAQLVDFKDNNPDKVP-EDVDYARMYKDVRAAVDLCHRDGTLKQMVAT 272
++T FS++EA +AQ+VD D+ +DY +YK V A+ H +G LK + +
Sbjct: 285 LNTFFSVSEALAYAQMVDRLDDGFISADLGTLDYKGLYKAVAKALFRAHVEGQLKSEIMS 344
Query: 273 DPKRFINMDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLDW 332
P+ F+ D + L +++G+ L+TNS + YT+ +M P+ ++DW
Sbjct: 345 KPELFVEPDPELPLALLDQKEAGKKLLLITNSDYHYTDKMMKHSFNKFLPN-----DMDW 404
Query: 333 LQYFDVVITGSAKPGFFHEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKVD 392
FD+VI + KP FF + PL +V P
Sbjct: 405 RDLFDMVIVSARKPEFFQMSH---------------------PLYEVVTGEGLMRPCFKA 464
Query: 393 KSCKVFQGGNVMHLHKLLQIESSSQVLYVGDHIYGDILRSKKALGWRTMLVVPELEREVE 452
++ ++ GG+ + L + ++LYVGDHIY D+ SK L WRT L+ ELE E
Sbjct: 465 ETGGLYSGGSAQMIESSLNVH-GDEILYVGDHIYTDVSVSKVHLRWRTALICRELEEEYM 524
Query: 453 LLWELRKTRKQLQLLRNDRDAVEDKIHHLKWSLK----------FEDVKADEKERLSAEL 512
L R R++L L N ++ V D + L+ +L+ D++E L+ +
Sbjct: 525 ALIGSRGHREELIELINQKEVVGDLFNQLRLALQRRSKGRPAQTLAATNLDDQE-LTETM 584
Query: 513 HQLESEQERVRLSHQDALRDCHRQFHGVWGQLMKTG-YQNSRFAHQVERFACLYSSQVSN 572
+L +R+ L F+ WG L + G + S Q+E++A +Y+S+VSN
Sbjct: 585 QKLLIVMQRLDDKIGLMLETDGELFNKRWGFLSRAGLWDKSHLMRQIEKYADIYTSRVSN 632
Query: 573 LSLYSPAKYYRPNEDFMPHE 581
Y+P Y+R E + H+
Sbjct: 645 FLNYTPFMYFRSQEQSLAHD 632
BLAST of CmaCh12G004730 vs. TAIR 10
Match:
AT2G23890.1 (HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase )
HSP 1 Score: 163.7 bits (413), Expect = 4.5e-40
Identity = 132/504 (26.19%), Postives = 220/504 (43.65%), Query Frame = 0
Query: 93 IFCNRSLNMKNIVAVGFDMDYTLAQYKPETFESLAYEGTIRKLVYDLAYSEELLNWSFDW 152
I+ N++L + NI GFD DYTLA Y +SL Y+ + +V + Y + + +D
Sbjct: 99 IYVNKNLRLDNIQVYGFDYDYTLAHYSSH-LQSLIYDLAKKHMVNEFRYPDVCTQFEYDP 158
Query: 153 KYMVRGLVLDKRRGNILKMDRHKYVKV--AYHGFKELSKDDKVETYGSTLVRDSFDEPDY 212
+ +RGL DK +G ++K+D ++ Y G ++LS+ + YG+ +
Sbjct: 159 TFPIRGLYYDKLKGCLMKLDFFGSIEPDGCYFGRRKLSRKEIESMYGTRHIGRDQARGLV 218
Query: 213 ALIDTLFSLAEAYLFAQLVDFKDNNPDKVPEDVDYARMYKDVRAAVDLCHRDGTLKQMVA 272
L+D F +EA L A +V + + + D + +Y DV A+ HR G + + +
Sbjct: 219 GLMD-FFCFSEACLIADMVQYFVD----AKLEFDASNIYNDVNRAIQHVHRSGLVHRGIL 278
Query: 273 TDPKRFINMDTSIVPMLKMLRDSGRSTFLVTNSLWDYTNVVMNFLCTSSAPDGDQKCNLD 332
DP R++ + ++ L+ML+D G+ FL+TNS +++ + M FL S GD
Sbjct: 279 ADPNRYLLKNGQLLRFLRMLKDKGKKLFLLTNSPYNFVDGGMRFLMEESFGFGD-----S 338
Query: 333 WLQYFDVVITGSAKPGFFHEDNRANLFGVDRKSGMLLNTDNGSPLPQVGNTSEASSPTKV 392
W + FDVVI + KP F+ ++ + +R + D P
Sbjct: 339 WRELFDVVIAKANKPEFYTSEHPFRCYDSERDNLAFTKVDAFDPK--------------- 398
Query: 393 DKSCKVFQGGNVMHLHKLLQIES--SSQVLYVGDHIYGDILRSKKALGWRTMLVVPELER 452
KV+ G L L+I +V+Y GDH++ D LR GWRT ++ ELER
Sbjct: 399 ----KVYYHG---CLKSFLEITKWHGPEVIYFGDHLFSD-LRGPSKAGWRTAAIIHELER 458
Query: 453 EVELLWELRKTRKQLQLLRNDRDAVEDKIHHLKWSLKFEDVKADEKERLSAELHQLESEQ 512
E++ ++ND S +FE K + L H S
Sbjct: 459 EIQ--------------IQNDD------------SYRFEQAKFHIIQELLGRFHATVSNN 518
Query: 513 ERVRL--SHQDALRDCHRQFHGVWGQLMKTGY---------QNSRFAHQVERFACLYSSQ 572
+R S D L + ++ Q+ + Q S F++ + ++A +Y+S+
Sbjct: 519 QRSEACQSLLDELNNARQRARDTMKQMFNRSFGATFVTDTGQESAFSYHIHQYADVYTSK 542
Query: 573 VSNLSLYSPAKYYRPNED--FMPH 580
N LY P + D MPH
Sbjct: 579 PENFLLYRPEAWLHVPYDIKIMPH 542
The following BLAST results are available for this feature:
Match Name | E-value | Identity | Description | |
Q3V1L4 | 7.2e-59 | 32.93 | Cytosolic purine 5'-nucleotidase OS=Mus musculus OX=10090 GN=Nt5c2 PE=1 SV=2 | [more] |
O46411 | 1.6e-58 | 32.73 | Cytosolic purine 5'-nucleotidase OS=Bos taurus OX=9913 GN=NT5C2 PE=1 SV=1 | [more] |
P49902 | 2.1e-58 | 32.73 | Cytosolic purine 5'-nucleotidase OS=Homo sapiens OX=9606 GN=NT5C2 PE=1 SV=1 | [more] |
Q5RA22 | 2.1e-58 | 32.73 | Cytosolic purine 5'-nucleotidase OS=Pongo abelii OX=9601 GN=NT5C2 PE=2 SV=1 | [more] |
Q54XC1 | 1.0e-57 | 31.75 | Cytosolic purine 5'-nucleotidase OS=Dictyostelium discoideum OX=44689 GN=nt5c2 P... | [more] |
Match Name | E-value | Identity | Description | |
A0A6J1KNI6 | 0.0e+00 | 100.00 | 5'-nucleotidase domain-containing protein 4-like isoform X1 OS=Cucurbita maxima ... | [more] |
A0A6J1GIA5 | 0.0e+00 | 96.93 | 5'-nucleotidase domain-containing protein 4-like isoform X1 OS=Cucurbita moschat... | [more] |
A0A6J1KKW6 | 0.0e+00 | 100.00 | 5'-nucleotidase domain-containing protein 4-like isoform X2 OS=Cucurbita maxima ... | [more] |
A0A6J1GIC9 | 0.0e+00 | 97.58 | 5'-nucleotidase domain-containing protein 4-like isoform X2 OS=Cucurbita moschat... | [more] |
A0A1S3BBU8 | 2.2e-310 | 87.86 | 5'-nucleotidase domain-containing protein 4 isoform X1 OS=Cucumis melo OX=3656 G... | [more] |
Match Name | E-value | Identity | Description | |
XP_023002285.1 | 0.0e+00 | 100.00 | 5'-nucleotidase domain-containing protein 4-like isoform X1 [Cucurbita maxima] | [more] |
XP_023537165.1 | 0.0e+00 | 97.61 | 5'-nucleotidase domain-containing protein 4-like isoform X1 [Cucurbita pepo subs... | [more] |
XP_022951662.1 | 0.0e+00 | 96.93 | 5'-nucleotidase domain-containing protein 4-like isoform X1 [Cucurbita moschata] | [more] |
KAG6585600.1 | 0.0e+00 | 96.04 | Cytosolic purine 5'-nucleotidase, partial [Cucurbita argyrosperma subsp. sororia... | [more] |
XP_023002286.1 | 0.0e+00 | 100.00 | 5'-nucleotidase domain-containing protein 4-like isoform X2 [Cucurbita maxima] | [more] |
Match Name | E-value | Identity | Description | |
AT1G75210.1 | 1.2e-242 | 69.90 | HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase | [more] |
AT5G48960.1 | 1.2e-56 | 30.80 | HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase | [more] |
AT2G23890.1 | 4.5e-40 | 26.19 | HAD-superfamily hydrolase, subfamily IG, 5'-nucleotidase | [more] |