CmaCh12G004630 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G004630
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectinesterase
Location	Cma_Chr12: 2418868 .. 2421073 (+)
RNA-Seq Expression	CmaCh12G004630
Synteny	CmaCh12G004630

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CAAACTCTTACTTCCAATTGCGTCGCCCTCAAGTTCATTGTCAAAATCTCACTCCGCCGGCGCCGCAGCTTCCATCAACGTCAAGCAACGCGCTCTTCAATCACCTATAAGAACGCTCCAACGATTGTTCGTAGTTCATCAATCATCATTGAAATCGAAATCATCTACGAGTTTTTATTTCTTTTCTCTTCACTTCCGAATCGAAATCTGTTGATTTCAAAATCCTACGAAAATGTCTCGTTCGCGCGGTTTCTTTCAAGCTTGTACAGCGATTGTGATTTGTGTGCTCGTGGCGGAGTTTACGGCGGCGATGGCGAAAACGGCGATTCTGATCACTGTCGATCAATCCGGTAGAGGAAATTTTACGAAGATTCAAAACGCGATTGATGCGGTGCCAGATAATAATTTGGAGGAAGTGTTTATTGCTGTGAAGCCAGGGATTTACAGAGAGAAGGTGACTGTACCAGCGAATAAACCGTACATTACGATCAGTGGAAGAAGATCGTCTGATACTATCATTACTTGGAATGATAGCAGAAATACATATAATTCCGCAACTCTTGCGGTTTTGGCCTCTGATTTCGTTGGACGATACCTCACGATTCAGGTTCTTAATTTCTACGGAACAAGAACTTTTGTTTTGATTTTATCTGATTTTGATTCATTCAAGCTTCCATGGCTTCGTTTGACGAAGATTATTCTCGTTTTGCAGCTTTTGTGAAATCTTCTTTTTAGTTTTTGTAATTTAGCTAATGTTTTTGAAACGCTTGCATGAACCACTGAGATTTTCCCCCAAATTAGTTAAATTATAAATTTAATCTTCAATAAAGTTATAAATCTTTAAATATCATAATTAATCTTTATTAATTTAAATTTTAAGTAATTTTTGCTAGTATCAAGTATTTCTTTATTATAAACTCATAATCATTTCTAAAGAATTTCATCTTCCAACAATTTTAACTATAAAAATTAAATTTCAATTCATTAGGGATTTTTTATTTTTATTTTTAGTAACAATTTATTTATATAATTATATATTTTTTATCTTAAAAAGGAAGCTAGTTGGGTATTAATCTATTCTGTCCTTATTATTTCAAATCTTTTAATATTATTTTATTTCCTAATAAGATAAAAGTATAAAAATAAAAAATAAAATATTATTCAATGGATTTTTTATTTTTATTCACTTAAGTAATTTTTATTTTAAAATTGTAAGATTTTTATATATATATATACTATTTTTTCAGAACGGGTTCGGGGCGGGTGCGCAAGCGGTGGCATTACGGGTATCCGGAGACCGAGTCTCATTTACCGCCTGCAGATTTCTGGGTCATCAAGACACTCTGCTAGACGACATCGGCCGCCATTACTACAAAAGCTGTTACATAGAAGGAGCCACCGACTTCATCTGCGGAAACGGGGCTTCACTTTTCGAGGTTCATTTTCTTTCCTTCTCTGTGAATTGGGCCTATTGGGCTATTGGGCCAAGAAAGCTTATTGACCCATTTGAGTTTGGGCTTTTGCAGAATTGCCATCTGCATTCGGTGTCAGACTCCGTCGGGACCATCACGGCTCAACGGAGAGAGTCGCCGTCGGAGAACACTGGGTTCGTGTTTATGGGTTGCAAGATCACCGGAATCAACTCCGCCGTTCTGGGTCGGCCGTGGGGGGCGTTCTCTAGGGTCGTGTTTGGATTCACTTACATGTCTGATGTTATTCTCCCTGAGGGTTGGGATAACTGGCAAGACCCATCCAAACAGAGGTTTCTATTCTTTCCTCCTTTTAAATTTTATTAATATTTAATAAATCCATTAATATTTGAAAATGTATTGGCAGCACTGTGTACTATGGACAATACCGATGTTACGGAAAAGGAGCCAATCCATCCAGAAGGGTACCATGGTCATTTTCCAACATGACCACCCAAGAAGCTGCACCATTCTTCTCCAAAACCTTCATTGGCGCTGCACATTGGCTCAGGCCTTTACCCACCAATTTCAAGAGAGCCTTCGCTTCCAAACCCTAAAAGAGTCAGTAATTACCATTCTATCCCTCTATATTCATTGTTTATACACTTGTATCTAAAACTCTCATAAATCAATAAATACTACTCCACGATTCTTTCCAATCTTCTTCTCACAAATTCTCTATATCGATCGGTATTCATTAAGTTATTGTTTTTATTCGACGTTTGTGTTGTTGTCTG

mRNA sequence

CAAACTCTTACTTCCAATTGCGTCGCCCTCAAGTTCATTGTCAAAATCTCACTCCGCCGGCGCCGCAGCTTCCATCAACGTCAAGCAACGCGCTCTTCAATCACCTATAAGAACGCTCCAACGATTGTTCGTAGTTCATCAATCATCATTGAAATCGAAATCATCTACGAGTTTTTATTTCTTTTCTCTTCACTTCCGAATCGAAATCTGTTGATTTCAAAATCCTACGAAAATGTCTCGTTCGCGCGGTTTCTTTCAAGCTTGTACAGCGATTGTGATTTGTGTGCTCGTGGCGGAGTTTACGGCGGCGATGGCGAAAACGGCGATTCTGATCACTGTCGATCAATCCGGTAGAGGAAATTTTACGAAGATTCAAAACGCGATTGATGCGGTGCCAGATAATAATTTGGAGGAAGTGTTTATTGCTGTGAAGCCAGGGATTTACAGAGAGAAGGTGACTGTACCAGCGAATAAACCGTACATTACGATCAGTGGAAGAAGATCGTCTGATACTATCATTACTTGGAATGATAGCAGAAATACATATAATTCCGCAACTCTTGCGGTTTTGGCCTCTGATTTCGTTGGACGATACCTCACGATTCAGAACGGGTTCGGGGCGGGTGCGCAAGCGGTGGCATTACGGGTATCCGGAGACCGAGTCTCATTTACCGCCTGCAGATTTCTGGGTCATCAAGACACTCTGCTAGACGACATCGGCCGCCATTACTACAAAAGCTGTTACATAGAAGGAGCCACCGACTTCATCTGCGGAAACGGGGCTTCACTTTTCGAGAATTGCCATCTGCATTCGGTGTCAGACTCCGTCGGGACCATCACGGCTCAACGGAGAGAGTCGCCGTCGGAGAACACTGGGTTCGTGTTTATGGGTTGCAAGATCACCGGAATCAACTCCGCCGTTCTGGGTCGGCCGTGGGGGGCGTTCTCTAGGGTCGTGTTTGGATTCACTTACATGTCTGATGTTATTCTCCCTGAGGGTTGGGATAACTGGCAAGACCCATCCAAACAGAGCACTGTGTACTATGGACAATACCGATGTTACGGAAAAGGAGCCAATCCATCCAGAAGGGTACCATGGTCATTTTCCAACATGACCACCCAAGAAGCTGCACCATTCTTCTCCAAAACCTTCATTGGCGCTGCACATTGGCTCAGGCCTTTACCCACCAATTTCAAGAGAGCCTTCGCTTCCAAACCCTAAAAGAGTCAGTAATTACCATTCTATCCCTCTATATTCATTGTTTATACACTTGTATCTAAAACTCTCATAAATCAATAAATACTACTCCACGATTCTTTCCAATCTTCTTCTCACAAATTCTCTATATCGATCGGTATTCATTAAGTTATTGTTTTTATTCGACGTTTGTGTTGTTGTCTG

Coding sequence (CDS)

ATGTCTCGTTCGCGCGGTTTCTTTCAAGCTTGTACAGCGATTGTGATTTGTGTGCTCGTGGCGGAGTTTACGGCGGCGATGGCGAAAACGGCGATTCTGATCACTGTCGATCAATCCGGTAGAGGAAATTTTACGAAGATTCAAAACGCGATTGATGCGGTGCCAGATAATAATTTGGAGGAAGTGTTTATTGCTGTGAAGCCAGGGATTTACAGAGAGAAGGTGACTGTACCAGCGAATAAACCGTACATTACGATCAGTGGAAGAAGATCGTCTGATACTATCATTACTTGGAATGATAGCAGAAATACATATAATTCCGCAACTCTTGCGGTTTTGGCCTCTGATTTCGTTGGACGATACCTCACGATTCAGAACGGGTTCGGGGCGGGTGCGCAAGCGGTGGCATTACGGGTATCCGGAGACCGAGTCTCATTTACCGCCTGCAGATTTCTGGGTCATCAAGACACTCTGCTAGACGACATCGGCCGCCATTACTACAAAAGCTGTTACATAGAAGGAGCCACCGACTTCATCTGCGGAAACGGGGCTTCACTTTTCGAGAATTGCCATCTGCATTCGGTGTCAGACTCCGTCGGGACCATCACGGCTCAACGGAGAGAGTCGCCGTCGGAGAACACTGGGTTCGTGTTTATGGGTTGCAAGATCACCGGAATCAACTCCGCCGTTCTGGGTCGGCCGTGGGGGGCGTTCTCTAGGGTCGTGTTTGGATTCACTTACATGTCTGATGTTATTCTCCCTGAGGGTTGGGATAACTGGCAAGACCCATCCAAACAGAGCACTGTGTACTATGGACAATACCGATGTTACGGAAAAGGAGCCAATCCATCCAGAAGGGTACCATGGTCATTTTCCAACATGACCACCCAAGAAGCTGCACCATTCTTCTCCAAAACCTTCATTGGCGCTGCACATTGGCTCAGGCCTTTACCCACCAATTTCAAGAGAGCCTTCGCTTCCAAACCCTAA

Protein sequence

MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHWLRPLPTNFKRAFASKP

Homology

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BLAST of CmaCh12G004630 vs. ExPASy Swiss-Prot
Match: Q9SIJ9 (Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1)

HSP 1 Score: 370.2 bits (949), Expect = 2.5e-101
Identity = 170/297 (57.24%), Postives = 224/297 (75.42%), Query Frame = 0


Query: 30  TAILITVDQSGRGNFTKIQNAIDAVPD--NNLEEVFIAVKPGIYREKVTVPANKPYITIS 89
           TAILI VDQSG+G+F+KIQ AI+++P   NN +  FI VKPGIYREKV +PA KPYIT+S
Sbjct: 47  TAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLS 106

Query: 90  GRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQNGFGAGAQAVALRVSGDRVSFT 149
           G ++S+T + W+D  +   S TL + ASDFV R+LTIQN FG   +AVALRV+ D+ +F 
Sbjct: 107 GTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKAAFY 166

Query: 150 ACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSVSDSVGTITAQRR 209
            C    +QDTLLDD G HY+K+CYIEGATDFICG+ +SL+E CHLHS+S + G+ITAQ R
Sbjct: 167 GCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITAQMR 226

Query: 210 ESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVILPEGWDNWQDPSKQS 269
            S +E +GF F+GCK+TG  S  LGRPWGA+SRVVF +++ S+V+ P+GW+ W D +K++
Sbjct: 227 TSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDSTKEN 286

Query: 270 TVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHWLRPLPTNFKRA 325
           TVYYG+Y+CYG GA+  +RV WS   ++ +EA  F SK FIG   WLRP P++FK A
Sbjct: 287 TVYYGEYKCYGPGADREQRVEWS-KQLSDEEATVFLSKDFIGGKDWLRPAPSHFKNA 342

[Close]

BLAST of CmaCh12G004630 vs. ExPASy Swiss-Prot
Match: Q8VYZ3 (Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1)

HSP 1 Score: 302.0 bits (772), Expect = 8.3e-81
Identity = 146/294 (49.66%), Postives = 191/294 (64.97%), Query Frame = 0


Query: 38  QSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIIT 97
           +S +G+FTKIQ+AID++P  N   V I V  G+Y+EKV++P  K +ITI G  +  T + 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 98  WNDS----------RNTYNSATLAVLASDFVGRYLTIQNGF------GAGAQAVALRVSG 157
           W D+            TYNSA+ AV +  FV + +T +N          G QAVALRVS 
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 158 DRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSVSDSVGT 217
           D  +F  CR LG QDTL D +GRHYYK CYIEG+ DFI GN  SL+E CH+H+++D +G 
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 218 ITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVILPEGWDNWQ 277
           +TAQ R S  E+TGF F+ CK+TG     LGR WG FSRVVF +TYM ++ILP GW NW 
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 278 DPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHWLR 316
           DPS++ TV+YGQY+C G GAN   RV W+   +T +EA PF S TFI  + W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWA-RELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of CmaCh12G004630 vs. ExPASy Swiss-Prot
Match: Q9ZQA3 (Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1)

HSP 1 Score: 270.8 bits (691), Expect = 2.1e-71
Identity = 138/302 (45.70%), Postives = 191/302 (63.25%), Query Frame = 0


Query: 29  KTAILITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYREKVTVPANKPYITISG 88
           KT++++TVD  G GNF+ +Q+AID VPD +  +  I V  G YREKVTV  NK  + I G
Sbjct: 86  KTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQG 145

Query: 89  RRSSDTIITWNDSR----NTYNSATLAVLASDFVGRYLTIQNGF------GAGAQAVALR 148
           R   +T I WND+     NT +S +  V A++F    ++ +N         A AQAVALR
Sbjct: 146 RGYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALR 205

Query: 149 VSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSV--- 208
           + GD+ +F  C F G QDTLLDD GRH++K C+I+G+ DFI GNG SL+++C ++S+   
Sbjct: 206 IEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKG 265

Query: 209 --SDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVIL 268
             S   G+ITAQ R+S  E +GF F+ CKI G    +LGR WGA++ VVF  TYMS +I 
Sbjct: 266 NTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIIT 325

Query: 269 PEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHW 316
           PEGW+NW D +K+ TV +G+++CYG GA+   RV +    +T  EA+ F   +FI    W
Sbjct: 326 PEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFG-KQLTDSEASSFIDVSFIDGDEW 385

[Close]

BLAST of CmaCh12G004630 vs. ExPASy Swiss-Prot
Match: Q8LPF3 (Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1)

HSP 1 Score: 268.5 bits (685), Expect = 1.0e-70
Identity = 128/301 (42.52%), Postives = 180/301 (59.80%), Query Frame = 0


Query: 33  LITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYREKVTVPANKPYITISGRRSS 92
           +ITV  +G   F  +Q+A+D++P NN + + I + PG YREKV VPA KPYIT  G    
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 93  DTIITWNDSRN----------TYNSATLAVLASDFVGRYLTIQNGFGA------GAQAVA 152
            T I W+D  +          TY +A++ V A+ F  R ++  N   A      G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 153 LRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSVS 212
            R+SGD+  F+ C F G QDTL DD GRHY+K CYIEG+ DFI GNG S++++C LHS++
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 213 DSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVILPEG 272
              G+I A  R  P E TGF F+GC++TG     +GR  G +SR+V+ +TY   ++   G
Sbjct: 240 SRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGG 299

Query: 273 WDNWQDPS-KQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHWLR 317
           WD+W   S K  T ++G Y CYG GA  +R V W+ + +  + A PF +K+F+   HW+ 
Sbjct: 300 WDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARA-LDYESAHPFIAKSFVNGRHWIA 359

[Close]

BLAST of CmaCh12G004630 vs. ExPASy Swiss-Prot
Match: Q9FM79 (Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1)

HSP 1 Score: 260.4 bits (664), Expect = 2.8e-68
Identity = 128/300 (42.67%), Postives = 179/300 (59.67%), Query Frame = 0


Query: 33  LITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYREKVTVPANKPYITISGRRS- 92
           +I VD++G G+   +Q A+D VPD+N + V I + PGIYREKV VP +KPYI+  G  S 
Sbjct: 81  VIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESY 140

Query: 93  -SDTIITWNDSRN----------TYNSATLAVLASDFVGRYLTIQNGFGA-----GAQAV 152
             DT+I+W+D  +          TY +A++++ +  F    +T +N   A     G QAV
Sbjct: 141 AGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAV 200

Query: 153 ALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSV 212
           ALR+ GD+  F   R LG QDTL DD G HY+  CYI+G  DFI GN  SL+++C +HS 
Sbjct: 201 ALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHST 260

Query: 213 SDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVILPE 272
           +   G I A  R+S +E+TGF F+ C I+G     LGR WG +SR V+   +++D+I P 
Sbjct: 261 AKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPV 320

Query: 273 GWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHWLR 316
           GW +W+ P +Q  V +G+Y C G+GA    RVPWS   +T  E  PF  + FI    WLR
Sbjct: 321 GWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWS-KTLTRDEVKPFLGREFIYGDQWLR 379

[Close]

BLAST of CmaCh12G004630 vs. ExPASy TrEMBL
Match: A0A6J1KQF0 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496269 PE=4 SV=1)

HSP 1 Score: 674.9 bits (1740), Expect = 1.7e-190
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLE 60
           MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLE
Sbjct: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLE 60

Query: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120
           EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR
Sbjct: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120

Query: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 180
           YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC
Sbjct: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 180

Query: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240
           GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR
Sbjct: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240

Query: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAA 300
           VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAA
Sbjct: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAA 300

Query: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 330
           PFFSKTFIGAAHWLRPLPTNFKRAFASKP
Sbjct: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

[Close]

BLAST of CmaCh12G004630 vs. ExPASy TrEMBL
Match: A0A6J1GIB5 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454415 PE=4 SV=1)

HSP 1 Score: 671.0 bits (1730), Expect = 2.5e-189
Identity = 326/329 (99.09%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLE 60
           MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVP+NNLE
Sbjct: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNLE 60

Query: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120
           EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR
Sbjct: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120

Query: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 180
           YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYY+SCYIEGATDFIC
Sbjct: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFIC 180

Query: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240
           GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR
Sbjct: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240

Query: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAA 300
           VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSF+NMTTQEAA
Sbjct: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAA 300

Query: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 330
           PFFSKTFIGAAHWLRPLPTNFKRAFASKP
Sbjct: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

[Close]

BLAST of CmaCh12G004630 vs. ExPASy TrEMBL
Match: A0A6J1CQY5 (Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111013943 PE=4 SV=1)

HSP 1 Score: 577.0 bits (1486), Expect = 5.0e-161
Identity = 277/320 (86.56%), Postives = 297/320 (92.81%), Query Frame = 0


Query: 10  ACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPG 69
           A  ++ + V VAE T A A  AILITVDQSGRGNFTKIQ+AIDAVP NN+E+VFIAVKPG
Sbjct: 14  ALISVFVAVFVAEITKAAATAAILITVDQSGRGNFTKIQDAIDAVPVNNVEQVFIAVKPG 73

Query: 70  IYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQNGFG 129
           IYREKV VPANKP+ITISGRR+ DTI+TWNDSR TYNSATLAVLASDFVGRYLTIQNGFG
Sbjct: 74  IYREKVIVPANKPFITISGRRAVDTIVTWNDSRITYNSATLAVLASDFVGRYLTIQNGFG 133

Query: 130 AGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFEN 189
            GAQAVALRVSGDRVSF ACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGN ASLFEN
Sbjct: 134 PGAQAVALRVSGDRVSFNACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNAASLFEN 193

Query: 190 CHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMS 249
           CHLHSVSD+VGTITAQRRESPSE TGF FMGCKITGINSAVLGRPWGA+SRVVFG+TYMS
Sbjct: 194 CHLHSVSDAVGTITAQRRESPSETTGFSFMGCKITGINSAVLGRPWGAYSRVVFGYTYMS 253

Query: 250 DVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIG 309
           D+ILP+GWD+WQDPSK STVYYGQYRCYGKGANPSRRVPWS +NMT+QEAAPFF+K+FIG
Sbjct: 254 DLILPQGWDDWQDPSKHSTVYYGQYRCYGKGANPSRRVPWSLTNMTSQEAAPFFNKSFIG 313

Query: 310 AAHWLRPLPTNFKRAFASKP 330
           AA WLRPLPT FKRAF+SKP
Sbjct: 314 AADWLRPLPTTFKRAFSSKP 333

[Close]

BLAST of CmaCh12G004630 vs. ExPASy TrEMBL
Match: A0A1S3BBW3 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103487980 PE=4 SV=1)

HSP 1 Score: 572.8 bits (1475), Expect = 9.3e-160
Identity = 277/325 (85.23%), Postives = 300/325 (92.31%), Query Frame = 0


Query: 4   SRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLEEVF 63
           SR  F    +++  VLVAE TAA   TA+LITVDQSG+GNFTKIQ AIDAVP+NN EEVF
Sbjct: 2   SRRSFLVVVSVIAAVLVAEVTAA-ETTAVLITVDQSGKGNFTKIQQAIDAVPENNREEVF 61

Query: 64  IAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLT 123
           I VK GIYREKV VPANKP+ITISGRR++DTII+WNDS+NTYNSATLAVLASDFVGRYLT
Sbjct: 62  IVVKAGIYREKVVVPANKPFITISGRRAADTIISWNDSKNTYNSATLAVLASDFVGRYLT 121

Query: 124 IQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNG 183
           IQN +GAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYI+GATDFICGN 
Sbjct: 122 IQNEYGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFICGNA 181

Query: 184 ASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF 243
           ASLFENCHL SVSD VGTITAQRRESPSENTGFVF+GCKITGINSAVLGRPWGAFSRVVF
Sbjct: 182 ASLFENCHLRSVSDDVGTITAQRRESPSENTGFVFLGCKITGINSAVLGRPWGAFSRVVF 241

Query: 244 GFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFF 303
           GFT+MSDVILPEGWDNWQDPSKQSTVYYGQY+CYGKGANPSRRV WSF+NMT Q+AAPFF
Sbjct: 242 GFTFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANPSRRVSWSFTNMTAQDAAPFF 301

Query: 304 SKTFIGAAHWLRPLPTNFKRAFASK 329
           +K+FIGAA WLRPLP +FKRAF+S+
Sbjct: 302 TKSFIGAADWLRPLPKHFKRAFSSR 325

[Close]

BLAST of CmaCh12G004630 vs. ExPASy TrEMBL
Match: A0A0A0LPB4 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G361760 PE=4 SV=1)

HSP 1 Score: 572.4 bits (1474), Expect = 1.2e-159
Identity = 276/326 (84.66%), Postives = 297/326 (91.10%), Query Frame = 0


Query: 4   SRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLEEVF 63
           SR  F    +++  VLVAE TAA   TA+LITVDQSG+GNFTKIQ AIDAVP NN EEVF
Sbjct: 2   SRPSFLLAISVIAAVLVAEVTAAAVTTAVLITVDQSGKGNFTKIQQAIDAVPINNKEEVF 61

Query: 64  IAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLT 123
           I+VK GIYREKV VPANKP+ITISGRR+ DTII+WNDS+NTYNSATLAVLASDFVGRYLT
Sbjct: 62  ISVKAGIYREKVVVPANKPFITISGRRAVDTIISWNDSKNTYNSATLAVLASDFVGRYLT 121

Query: 124 IQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNG 183
           IQNG+G GAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYI+GATDFICGN 
Sbjct: 122 IQNGYGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFICGNA 181

Query: 184 ASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF 243
           ASLFENCHL SVS+ VGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF
Sbjct: 182 ASLFENCHLRSVSEDVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVF 241

Query: 244 GFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFF 303
           GFT+MSDVILPEGWDNWQDPSKQSTVYYGQY+CYGKGAN SRRV WSF+NMT Q+AAPFF
Sbjct: 242 GFTFMSDVILPEGWDNWQDPSKQSTVYYGQYKCYGKGANTSRRVSWSFTNMTAQDAAPFF 301

Query: 304 SKTFIGAAHWLRPLPTNFKRAFASKP 330
           +K+FIGAA WLRP+P  FKRAF+S P
Sbjct: 302 TKSFIGAADWLRPVPNRFKRAFSSHP 327

[Close]

BLAST of CmaCh12G004630 vs. NCBI nr
Match: XP_023002424.1 (putative pectinesterase 11 [Cucurbita maxima])

HSP 1 Score: 674.9 bits (1740), Expect = 3.6e-190
Identity = 329/329 (100.00%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLE 60
           MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLE
Sbjct: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLE 60

Query: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120
           EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR
Sbjct: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120

Query: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 180
           YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC
Sbjct: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 180

Query: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240
           GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR
Sbjct: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240

Query: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAA 300
           VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAA
Sbjct: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAA 300

Query: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 330
           PFFSKTFIGAAHWLRPLPTNFKRAFASKP
Sbjct: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

[Close]

BLAST of CmaCh12G004630 vs. NCBI nr
Match: XP_022951672.1 (putative pectinesterase 11 [Cucurbita moschata])

HSP 1 Score: 671.0 bits (1730), Expect = 5.2e-189
Identity = 326/329 (99.09%), Postives = 329/329 (100.00%), Query Frame = 0


Query: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLE 60
           MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVP+NNLE
Sbjct: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNLE 60

Query: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120
           EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR
Sbjct: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120

Query: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 180
           YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYY+SCYIEGATDFIC
Sbjct: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFIC 180

Query: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240
           GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR
Sbjct: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240

Query: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAA 300
           VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSF+NMTTQEAA
Sbjct: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAA 300

Query: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 330
           PFFSKTFIGAAHWLRPLPTNFKRAFASKP
Sbjct: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

[Close]

BLAST of CmaCh12G004630 vs. NCBI nr
Match: KAG6585588.1 (putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. sororia] >KAG7020503.1 putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 669.5 bits (1726), Expect = 1.5e-188
Identity = 325/329 (98.78%), Postives = 328/329 (99.70%), Query Frame = 0


Query: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLE 60
           MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAID VP+NNLE
Sbjct: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDVVPENNLE 60

Query: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120
           EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR
Sbjct: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120

Query: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 180
           YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYY+SCYIEGATDFIC
Sbjct: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYRSCYIEGATDFIC 180

Query: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240
           GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR
Sbjct: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240

Query: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAA 300
           VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSF+NMTTQEAA
Sbjct: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAA 300

Query: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 330
           PFFSKTFIGAAHWLRPLPTNFKRAFASKP
Sbjct: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

[Close]

BLAST of CmaCh12G004630 vs. NCBI nr
Match: XP_023536774.1 (putative pectinesterase 11 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 667.5 bits (1721), Expect = 5.8e-188
Identity = 325/329 (98.78%), Postives = 327/329 (99.39%), Query Frame = 0


Query: 1   MSRSRGFFQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLE 60
           MS SRGF QACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVP+NNLE
Sbjct: 1   MSSSRGFLQACTAIVICVLVAEFTAAMAKTAILITVDQSGRGNFTKIQNAIDAVPENNLE 60

Query: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120
           EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR
Sbjct: 61  EVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGR 120

Query: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 180
           YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC
Sbjct: 121 YLTIQNGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFIC 180

Query: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240
           GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR
Sbjct: 181 GNGASLFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSR 240

Query: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAA 300
           VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSF+NMTTQEAA
Sbjct: 241 VVFGFTYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFTNMTTQEAA 300

Query: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 330
           PFFSKTFIGAAHWLRPLPTNFKRAFASKP
Sbjct: 301 PFFSKTFIGAAHWLRPLPTNFKRAFASKP 329

[Close]

BLAST of CmaCh12G004630 vs. NCBI nr
Match: XP_038884203.1 (putative pectinesterase 11 [Benincasa hispida])

HSP 1 Score: 582.0 bits (1499), Expect = 3.2e-162
Identity = 282/324 (87.04%), Postives = 304/324 (93.83%), Query Frame = 0


Query: 8   FQACTAIVICVLVAEFT--AAMAKTAILITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIA 67
           F    +++  VL+AE T  AA AKTAILITVDQSG+GNFTKIQ AIDAVP+NN EEVFIA
Sbjct: 6   FLVAISVLAAVLLAEVTAAAAAAKTAILITVDQSGKGNFTKIQEAIDAVPENNREEVFIA 65

Query: 68  VKPGIYREKVTVPANKPYITISGRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQ 127
           VK GIYREK+ VPANKPYITISGR ++DTII+WNDS+NTYNSATLAVLASDFVGRYLTIQ
Sbjct: 66  VKAGIYREKLVVPANKPYITISGRTAADTIISWNDSKNTYNSATLAVLASDFVGRYLTIQ 125

Query: 128 NGFGAGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGAS 187
           N FG GAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYI+GATDFICGN AS
Sbjct: 126 NEFGPGAQAVALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIQGATDFICGNAAS 185

Query: 188 LFENCHLHSVSDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGF 247
           LFENCHL SVSD+VGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGF
Sbjct: 186 LFENCHLRSVSDAVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGF 245

Query: 248 TYMSDVILPEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSK 307
           TYMSD+ILPEGWD+WQDPSKQSTV+YGQYRCYGKGANPSRRVPWSF+NMTT+EAAPFF+K
Sbjct: 246 TYMSDLILPEGWDDWQDPSKQSTVFYGQYRCYGKGANPSRRVPWSFTNMTTEEAAPFFTK 305

Query: 308 TFIGAAHWLRPLPTNFKRAFASKP 330
           +FIGAA WLRPLPT+FKRAF+SKP
Sbjct: 306 SFIGAADWLRPLPTHFKRAFSSKP 329

[Close]

BLAST of CmaCh12G004630 vs. TAIR 10
Match: AT2G21610.1 (pectinesterase 11 )

HSP 1 Score: 370.2 bits (949), Expect = 1.8e-102
Identity = 170/297 (57.24%), Postives = 224/297 (75.42%), Query Frame = 0


Query: 30  TAILITVDQSGRGNFTKIQNAIDAVPD--NNLEEVFIAVKPGIYREKVTVPANKPYITIS 89
           TAILI VDQSG+G+F+KIQ AI+++P   NN +  FI VKPGIYREKV +PA KPYIT+S
Sbjct: 47  TAILIRVDQSGKGDFSKIQEAIESIPPNLNNSQLYFIWVKPGIYREKVVIPAEKPYITLS 106

Query: 90  GRRSSDTIITWNDSRNTYNSATLAVLASDFVGRYLTIQNGFGAGAQAVALRVSGDRVSFT 149
           G ++S+T + W+D  +   S TL + ASDFV R+LTIQN FG   +AVALRV+ D+ +F 
Sbjct: 107 GTQASNTFLIWSDGEDILESPTLTIFASDFVCRFLTIQNKFGTAGRAVALRVAADKAAFY 166

Query: 150 ACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSVSDSVGTITAQRR 209
            C    +QDTLLDD G HY+K+CYIEGATDFICG+ +SL+E CHLHS+S + G+ITAQ R
Sbjct: 167 GCVITSYQDTLLDDNGNHYFKNCYIEGATDFICGSASSLYERCHLHSLSPNNGSITAQMR 226

Query: 210 ESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVILPEGWDNWQDPSKQS 269
            S +E +GF F+GCK+TG  S  LGRPWGA+SRVVF +++ S+V+ P+GW+ W D +K++
Sbjct: 227 TSATEKSGFTFLGCKLTGSGSTFLGRPWGAYSRVVFAYSFFSNVVAPQGWNQWGDSTKEN 286

Query: 270 TVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHWLRPLPTNFKRA 325
           TVYYG+Y+CYG GA+  +RV WS   ++ +EA  F SK FIG   WLRP P++FK A
Sbjct: 287 TVYYGEYKCYGPGADREQRVEWS-KQLSDEEATVFLSKDFIGGKDWLRPAPSHFKNA 342

[Close]

BLAST of CmaCh12G004630 vs. TAIR 10
Match: AT5G19730.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 302.0 bits (772), Expect = 5.9e-82
Identity = 146/294 (49.66%), Postives = 191/294 (64.97%), Query Frame = 0


Query: 38  QSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYREKVTVPANKPYITISGRRSSDTIIT 97
           +S +G+FTKIQ+AID++P  N   V I V  G+Y+EKV++P  K +ITI G  +  T + 
Sbjct: 90  KSNKGDFTKIQDAIDSLPLINFVRVVIKVHAGVYKEKVSIPPLKAFITIEGEGAEKTTVE 149

Query: 98  WNDS----------RNTYNSATLAVLASDFVGRYLTIQNGF------GAGAQAVALRVSG 157
           W D+            TYNSA+ AV +  FV + +T +N          G QAVALRVS 
Sbjct: 150 WGDTAQTPDSKGNPMGTYNSASFAVNSPFFVAKNITFRNTTPVPLPGAVGKQAVALRVSA 209

Query: 158 DRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSVSDSVGT 217
           D  +F  CR LG QDTL D +GRHYYK CYIEG+ DFI GN  SL+E CH+H+++D +G 
Sbjct: 210 DNAAFFGCRMLGAQDTLYDHLGRHYYKDCYIEGSVDFIFGNALSLYEGCHVHAIADKLGA 269

Query: 218 ITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVILPEGWDNWQ 277
           +TAQ R S  E+TGF F+ CK+TG     LGR WG FSRVVF +TYM ++ILP GW NW 
Sbjct: 270 VTAQGRSSVLEDTGFSFVKCKVTGTGVLYLGRAWGPFSRVVFAYTYMDNIILPRGWYNWG 329

Query: 278 DPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHWLR 316
           DPS++ TV+YGQY+C G GAN   RV W+   +T +EA PF S TFI  + W++
Sbjct: 330 DPSREMTVFYGQYKCTGAGANYGGRVAWA-RELTDEEAKPFLSLTFIDGSEWIK 382

[Close]

BLAST of CmaCh12G004630 vs. TAIR 10
Match: AT2G36710.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 270.8 bits (691), Expect = 1.5e-72
Identity = 138/302 (45.70%), Postives = 191/302 (63.25%), Query Frame = 0


Query: 29  KTAILITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYREKVTVPANKPYITISG 88
           KT++++TVD  G GNF+ +Q+AID VPD +  +  I V  G YREKVTV  NK  + I G
Sbjct: 86  KTSLVLTVDLHGCGNFSNVQSAIDVVPDLSSSKTLIIVNSGSYREKVTVNENKTNLVIQG 145

Query: 89  RRSSDTIITWNDSR----NTYNSATLAVLASDFVGRYLTIQNGF------GAGAQAVALR 148
           R   +T I WND+     NT +S +  V A++F    ++ +N         A AQAVALR
Sbjct: 146 RGYQNTSIEWNDTAKSAGNTADSFSFVVFAANFTAYNISFKNNAPEPDPGEADAQAVALR 205

Query: 149 VSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSV--- 208
           + GD+ +F  C F G QDTLLDD GRH++K C+I+G+ DFI GNG SL+++C ++S+   
Sbjct: 206 IEGDQAAFYGCGFYGAQDTLLDDKGRHFFKECFIQGSIDFIFGNGRSLYQDCTINSIAKG 265

Query: 209 --SDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVIL 268
             S   G+ITAQ R+S  E +GF F+ CKI G    +LGR WGA++ VVF  TYMS +I 
Sbjct: 266 NTSGVTGSITAQGRQSEDEQSGFSFVNCKIDGSGEILLGRAWGAYATVVFSNTYMSGIIT 325

Query: 269 PEGWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHW 316
           PEGW+NW D +K+ TV +G+++CYG GA+   RV +    +T  EA+ F   +FI    W
Sbjct: 326 PEGWNNWGDSTKEKTVTFGEHKCYGPGADYKERVLFG-KQLTDSEASSFIDVSFIDGDEW 385

[Close]

BLAST of CmaCh12G004630 vs. TAIR 10
Match: AT5G47500.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 268.5 bits (685), Expect = 7.3e-72
Identity = 128/301 (42.52%), Postives = 180/301 (59.80%), Query Frame = 0


Query: 33  LITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYREKVTVPANKPYITISGRRSS 92
           +ITV  +G   F  +Q+A+D++P NN + + I + PG YREKV VPA KPYIT  G    
Sbjct: 60  VITVSLNGHAQFRSVQDAVDSIPKNNNKSITIKIAPGFYREKVVVPATKPYITFKGAGRD 119

Query: 93  DTIITWNDSRN----------TYNSATLAVLASDFVGRYLTIQNGFGA------GAQAVA 152
            T I W+D  +          TY +A++ V A+ F  R ++  N   A      G QAVA
Sbjct: 120 VTAIEWHDRASDLGANGQQLRTYQTASVTVYANYFTARNISFTNTAPAPLPGMQGWQAVA 179

Query: 153 LRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSVS 212
            R+SGD+  F+ C F G QDTL DD GRHY+K CYIEG+ DFI GNG S++++C LHS++
Sbjct: 180 FRISGDKAFFSGCGFYGAQDTLCDDAGRHYFKECYIEGSIDFIFGNGRSMYKDCELHSIA 239

Query: 213 DSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVILPEG 272
              G+I A  R  P E TGF F+GC++TG     +GR  G +SR+V+ +TY   ++   G
Sbjct: 240 SRFGSIAAHGRTCPEEKTGFAFVGCRVTGTGPLYVGRAMGQYSRIVYAYTYFDALVAHGG 299

Query: 273 WDNWQDPS-KQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHWLR 317
           WD+W   S K  T ++G Y CYG GA  +R V W+ + +  + A PF +K+F+   HW+ 
Sbjct: 300 WDDWDHKSNKSKTAFFGVYNCYGPGAAATRGVSWARA-LDYESAHPFIAKSFVNGRHWIA 359

[Close]

BLAST of CmaCh12G004630 vs. TAIR 10
Match: AT5G55590.1 (Pectin lyase-like superfamily protein )

HSP 1 Score: 260.4 bits (664), Expect = 2.0e-69
Identity = 128/300 (42.67%), Postives = 179/300 (59.67%), Query Frame = 0


Query: 33  LITVDQSGRGNFTKIQNAIDAVPDNNLEEVFIAVKPGIYREKVTVPANKPYITISGRRS- 92
           +I VD++G G+   +Q A+D VPD+N + V I + PGIYREKV VP +KPYI+  G  S 
Sbjct: 81  VIVVDKNGGGDSVTVQGAVDMVPDSNSQRVKIFILPGIYREKVIVPKSKPYISFIGNESY 140

Query: 93  -SDTIITWNDSRN----------TYNSATLAVLASDFVGRYLTIQNGFGA-----GAQAV 152
             DT+I+W+D  +          TY +A++++ +  F    +T +N   A     G QAV
Sbjct: 141 AGDTVISWSDKASDLGCDGKELGTYRTASVSIESDFFCATAITFENTVVAEAGEQGRQAV 200

Query: 153 ALRVSGDRVSFTACRFLGHQDTLLDDIGRHYYKSCYIEGATDFICGNGASLFENCHLHSV 212
           ALR+ GD+  F   R LG QDTL DD G HY+  CYI+G  DFI GN  SL+++C +HS 
Sbjct: 201 ALRIIGDKAVFYRVRVLGSQDTLFDDNGSHYFYQCYIQGNVDFIFGNAKSLYQDCDIHST 260

Query: 213 SDSVGTITAQRRESPSENTGFVFMGCKITGINSAVLGRPWGAFSRVVFGFTYMSDVILPE 272
           +   G I A  R+S +E+TGF F+ C I+G     LGR WG +SR V+   +++D+I P 
Sbjct: 261 AKRYGAIAAHHRDSETEDTGFSFVNCDISGTGQIYLGRAWGNYSRTVYSNCFIADIITPV 320

Query: 273 GWDNWQDPSKQSTVYYGQYRCYGKGANPSRRVPWSFSNMTTQEAAPFFSKTFIGAAHWLR 316
           GW +W+ P +Q  V +G+Y C G+GA    RVPWS   +T  E  PF  + FI    WLR
Sbjct: 321 GWSDWKHPERQRKVMFGEYNCRGRGAERGGRVPWS-KTLTRDEVKPFLGREFIYGDQWLR 379

The following BLAST results are available for this feature:

BLAST of CmaCh12G004630 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SIJ9	2.5e-101	57.24	Putative pectinesterase 11 OS=Arabidopsis thaliana OX=3702 GN=PME11 PE=3 SV=1	[more]
Q8VYZ3	8.3e-81	49.66	Probable pectinesterase 53 OS=Arabidopsis thaliana OX=3702 GN=PME53 PE=2 SV=1	[more]
Q9ZQA3	2.1e-71	45.70	Probable pectinesterase 15 OS=Arabidopsis thaliana OX=3702 GN=PME15 PE=2 SV=1	[more]
Q8LPF3	1.0e-70	42.52	Probable pectinesterase 68 OS=Arabidopsis thaliana OX=3702 GN=PME68 PE=2 SV=1	[more]
Q9FM79	2.8e-68	42.67	Pectinesterase QRT1 OS=Arabidopsis thaliana OX=3702 GN=QRT1 PE=2 SV=1	[more]

BLAST of CmaCh12G004630 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KQF0	1.7e-190	100.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111496269 PE=4 SV=1	[more]
A0A6J1GIB5	2.5e-189	99.09	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111454415 PE=4 SV=1	[more]
A0A6J1CQY5	5.0e-161	86.56	Pectinesterase OS=Momordica charantia OX=3673 GN=LOC111013943 PE=4 SV=1	[more]
A0A1S3BBW3	9.3e-160	85.23	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103487980 PE=4 SV=1	[more]
A0A0A0LPB4	1.2e-159	84.66	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_2G361760 PE=4 SV=1	[more]

BLAST of CmaCh12G004630 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023002424.1	3.6e-190	100.00	putative pectinesterase 11 [Cucurbita maxima]	[more]
XP_022951672.1	5.2e-189	99.09	putative pectinesterase 11 [Cucurbita moschata]	[more]
KAG6585588.1	1.5e-188	98.78	putative pectinesterase 11, partial [Cucurbita argyrosperma subsp. sororia] >KAG...	[more]
XP_023536774.1	5.8e-188	98.78	putative pectinesterase 11 [Cucurbita pepo subsp. pepo]	[more]
XP_038884203.1	3.2e-162	87.04	putative pectinesterase 11 [Benincasa hispida]	[more]

BLAST of CmaCh12G004630 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G21610.1	1.8e-102	57.24	pectinesterase 11	[more]
AT5G19730.1	5.9e-82	49.66	Pectin lyase-like superfamily protein	[more]
AT2G36710.1	1.5e-72	45.70	Pectin lyase-like superfamily protein	[more]
AT5G47500.1	7.3e-72	42.52	Pectin lyase-like superfamily protein	[more]
AT5G55590.1	2.0e-69	42.67	Pectin lyase-like superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 28..324 e-value: 2.0E-109 score: 367.9
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 34..309 e-value: 2.3E-73 score: 246.5
None	No IPR available	PANTHER	PTHR31321:SF77	PECTINESTERASE 11-RELATED	coord: 14..315
None	No IPR available	PANTHER	PTHR31321	ACYL-COA THIOESTER HYDROLASE YBHC-RELATED	coord: 14..315
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 29..321

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G004630.1	CmaCh12G004630.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0046274	lignin catabolic process
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0048046	apoplast
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0005507	copper ion binding
molecular_function	GO:0052716	hydroquinone:oxygen oxidoreductase activity
molecular_function	GO:0030599	pectinesterase activity