CmaCh12G004550 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G004550
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionProteasome subunit alpha type
LocationCma_Chr12: 2395134 .. 2398477 (+)
RNA-Seq ExpressionCmaCh12G004550
SyntenyCmaCh12G004550
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCGTCGCCAAATTTTGCCTTGAAGAAGACGAAAACCCCTAAATCCTCCATTAAGGCGACCTCAATCCTCTTCAGTTTCTATCGGAGAGATCGTGATTGTGGCTCTGCGAACCTCTTGAATCCGCCTTACCTTCACCAAAAATGTTTAGGAACCAGTACGATACGGACGTGACGACATGGAGCCCGGCGGGGAGGTTGTTTCAGGTTGAGTACGCGATGGAGGCTGTGAAACAAGGCTCCGCTGCCATTGGTCTCCGATCCAAGACTCATGTGGTTCTGGCCTGCGTCAATAAGGCTAATTCTGAACTATCCTCGCACCAGAAGAAGATCTTCAAAGTCGATGATCACATCGGCGTCGCCATTTCCGGGCTTACTGCTGATGGACGCGTTCTTTCCCGTTATATGAGATCTGAGTGTATCAATTATAGTTACACTTATGAATCTCCTCTTCCTGTTGGTCGTCTTGTAGTTCAACTTGCCGATAAGGCTCAGGTTTGTAAACGAAGCCCTTGTTTTCTTCACAATTTTTCTGGCGCTTTGGATTTCTTTTGCATTTTAGATATGTCAGCCGTGTGAGTATTTATTTGGTTAGTGGGGGGCTTGGTACAGAAATTACGAATTAATGTAGCTGGATGGATAGTTAAAGGGGAAGTTTTCATTAAGCTCTCAAAGCTAAGGAATACAATGTCGAGTTTATCTTTAGTCAGTAGACGAGATTATGGAACATTTGGTAAATGGCAGAAGGTCTCCTCTGTGTACTCTATTTTACAGCATTTTGAAGACAGCTAAAGATTATAGGCTTTCTGTTTGCTTCTTATCTTATATAGTTAGTGTTTGTTCCACTGATTCTTGATCGCGTAGTGGTAGAAGAAAACCAGGAATTTTAGGTCTTTAACTTGTTTGATTATTCTAATTGTTGAATTACTTTGAACCTGCCTATCGTTGCTGGTAAGTTTTTATTTTCTTTCTCAAGTATTGTTGTTTAGTAGTTTAGGTTTTAGTAGAAGCCAGGGGGCTGTGTAATTATTTAAGGTCACAATGTATCTGCCTACCACTTTTTGTGGCTCTTGGATATATACGAGATTAAAGAATGCTTTGAACTATGTATCTATCTGATTGTCATGGGGTTGGATAGCTCTCAATAACTTTTCCTGTGAAGGTTTTCTTAATAGAGTCATACAAGTTGAGTACTCTTATATGCACGTATATGTCACGTGATTGATGGACTGAGTACGGAACAAGAAACGTATGCAGCTGGATGATTGAGGGCACGGTCTCCATTATATTAGGCTCCAACATTCTGCACTGTCTTTGATCACCTTGTATTTTGAGAATTGGGGGTTTAATTAATTAGTATACAATGGGGCACTGAGAGATAGCATGGGGATTGGGAATTCTCTACCTCCAATTTCAAGGAAAAGAAGTTAAAGTTCAATTTCATTTAATTGTAGTTGAGCCGTGTCGGTGTTATTGGTTTAAATAGATCCTTTATAAGGTTAGATTCTGCTTTCAAGTTCTGTGGTTTATCTAATTATTTTGATTTCTCAATTCCTTTGTACTGCCATAGTTTTAGCTTTGTTGACAATTTAGGCAATAAAATGTTGGTATTCATAATGCAAGACTCATATTATAGTGTAGATGCAGAAACAATCTTGGTAACCTGACTTATTGCATTTATTCCCACTTTTGTTGTTAATAAGAAACATTGTGAATACACGGAGTGGGAATAAATGGCATCCACAGCAAGCTAATAATACATGTAGACCTTCCAAACTAGGTTATGTTACAGAATAAACAATACAGTATAGAACAAAAGGCATTCTAACAAGAGGCTGTTTAAAGAACAAAATCACTAAAGTTATGTAAAGTTACTTCCAAGGGGCTCTATCTTTTTCTTTCAGCCACCTCTGTGAGGAGTCTATCTTTTATGCATTTGCGAAGGAACACCGAAAATTGCATTCACTCCTCTTTAACCCCTATTTTTTTCTGCCGTAAATTAGGTTCATTAACAAATTACGTTCATTAACCAATTAGGTTACTACCATTTTGGTGTACCCTTTTCTTTCTTTGTAGAGACAGATTTCAATTGATTTGGGCAGAAATTTTTGATAAATCACGGTGAATTGCTTTCCACAATAATATTTTGAAGTGTTCAGGATATACCTTATATCGTTGCACGGGATGTGTAGTTGTTAATTGTCATTTATCTTTTGCTGGTGCCTCCTTGAATCATTTAGGTGTCTGCTCCTAGGCTGTAGGTAGGATTCAGCACAATTTCAAAAGCCATGCCTTCATGCTCCCTGTATAGTGGGTTCCTATCGTTTCCTCTATTAGAAAATTTATCCATCTTTCCTAGGATGTGCTAAAATTCTAGAGCGCTGAAACAGGACTTCAGGCTTGATATACTTTGTTAAGGGTATAATCTCTCTGAACTTTTAATATTTTTTGTATTTCCAGGACTCTATGCTGGTTATTTTCACAGGAGAAACTAGTTTTGAGGTTTAGAAATGTTTGCTTTCTCTAAAAATGTTGGCCACTGATTGCAGGTCTGCACCCAGCGCTCGTGGAAACGTCCATATGGTGTTGGTCTGCTAGTAGGTGGTCTGGATGAGTCTGGTGCTCACCTTTATTACAACTGCCCCAGTGGGAATTACTTTGAATATCAAGCTTTTGCCATAGGATCCCGTTCACAGGCTGCAAAGACATACTTGGAAAGGAGGTTTGAGAACTTCAAGGACTCTTCTAGAGACGACTTGATCAAAGATGCACTGATCGCCACCAGAGAAACCTTGCAAGGAGAAAAACTGACTTCCTCCATATGCACAATTGCCATCTTAGGAGTTGGGGAACCTTTTCATATATTGGATAGGGAAACTGTTCAGAAGTTGATCAATGAATTCGAGATCGTTAAGGAGGATGACGTCCCTGCTGCAGATCAGGATATCGAGCCAGATCAGGGTGATTCTGCTGCTGCTGCTGCCCCTGAACCAGCTGCAAGGACAGACCAAGGTAGTGCCCCGGAGCAAGGCGTGGCACCAATGGACATCTAACCCACATCCCTTCCTCCTCCCCCGCTTCCTGGGTGTTGCTGTCAGGTTTGGTGGTTTGGTTCTTTAGTGCAGACATTTTGATACTTCTCTGTTGTGGCTTAAGTTTTCAATTTTACATTTATGGAAGTTGTATCAGACTGTTGAAAACGTGTTATCACAACTGATTATGTTTTTCTATTATATTATTGGGAGAAGAACGTTGAGCTGATCCACGATGTTCTTTAGAAACTTGGAATCAAATTAGTGTTAGATTGAAACTTGAGGTGAAAATGATATTGAATTGCATGAAAAGGAG

mRNA sequence

TCGTCGCCAAATTTTGCCTTGAAGAAGACGAAAACCCCTAAATCCTCCATTAAGGCGACCTCAATCCTCTTCAGTTTCTATCGGAGAGATCGTGATTGTGGCTCTGCGAACCTCTTGAATCCGCCTTACCTTCACCAAAAATGTTTAGGAACCAGTACGATACGGACGTGACGACATGGAGCCCGGCGGGGAGGTTGTTTCAGGTTGAGTACGCGATGGAGGCTGTGAAACAAGGCTCCGCTGCCATTGGTCTCCGATCCAAGACTCATGTGGTTCTGGCCTGCGTCAATAAGGCTAATTCTGAACTATCCTCGCACCAGAAGAAGATCTTCAAAGTCGATGATCACATCGGCGTCGCCATTTCCGGGCTTACTGCTGATGGACGCGTTCTTTCCCGTTATATGAGATCTGAGTGTATCAATTATAGTTACACTTATGAATCTCCTCTTCCTGTTGGTCGTCTTGTAGTTCAACTTGCCGATAAGGCTCAGGTCTGCACCCAGCGCTCGTGGAAACGTCCATATGGTGTTGGTCTGCTAGTAGGTGGTCTGGATGAGTCTGGTGCTCACCTTTATTACAACTGCCCCAGTGGGAATTACTTTGAATATCAAGCTTTTGCCATAGGATCCCGTTCACAGGCTGCAAAGACATACTTGGAAAGGAGGTTTGAGAACTTCAAGGACTCTTCTAGAGACGACTTGATCAAAGATGCACTGATCGCCACCAGAGAAACCTTGCAAGGAGAAAAACTGACTTCCTCCATATGCACAATTGCCATCTTAGGAGTTGGGGAACCTTTTCATATATTGGATAGGGAAACTGTTCAGAAGTTGATCAATGAATTCGAGATCGTTAAGGAGGATGACGTCCCTGCTGCAGATCAGGATATCGAGCCAGATCAGGGTGATTCTGCTGCTGCTGCTGCCCCTGAACCAGCTGCAAGGACAGACCAAGGTAGTGCCCCGGAGCAAGGCGTGGCACCAATGGACATCTAACCCACATCCCTTCCTCCTCCCCCGCTTCCTGGGTGTTGCTGTCAGGTTTGGTGGTTTGGTTCTTTAGTGCAGACATTTTGATACTTCTCTGTTGTGGCTTAAGTTTTCAATTTTACATTTATGGAAGTTGTATCAGACTGTTGAAAACGTGTTATCACAACTGATTATGTTTTTCTATTATATTATTGGGAGAAGAACGTTGAGCTGATCCACGATGTTCTTTAGAAACTTGGAATCAAATTAGTGTTAGATTGAAACTTGAGGTGAAAATGATATTGAATTGCATGAAAAGGAG

Coding sequence (CDS)

ATGTTTAGGAACCAGTACGATACGGACGTGACGACATGGAGCCCGGCGGGGAGGTTGTTTCAGGTTGAGTACGCGATGGAGGCTGTGAAACAAGGCTCCGCTGCCATTGGTCTCCGATCCAAGACTCATGTGGTTCTGGCCTGCGTCAATAAGGCTAATTCTGAACTATCCTCGCACCAGAAGAAGATCTTCAAAGTCGATGATCACATCGGCGTCGCCATTTCCGGGCTTACTGCTGATGGACGCGTTCTTTCCCGTTATATGAGATCTGAGTGTATCAATTATAGTTACACTTATGAATCTCCTCTTCCTGTTGGTCGTCTTGTAGTTCAACTTGCCGATAAGGCTCAGGTCTGCACCCAGCGCTCGTGGAAACGTCCATATGGTGTTGGTCTGCTAGTAGGTGGTCTGGATGAGTCTGGTGCTCACCTTTATTACAACTGCCCCAGTGGGAATTACTTTGAATATCAAGCTTTTGCCATAGGATCCCGTTCACAGGCTGCAAAGACATACTTGGAAAGGAGGTTTGAGAACTTCAAGGACTCTTCTAGAGACGACTTGATCAAAGATGCACTGATCGCCACCAGAGAAACCTTGCAAGGAGAAAAACTGACTTCCTCCATATGCACAATTGCCATCTTAGGAGTTGGGGAACCTTTTCATATATTGGATAGGGAAACTGTTCAGAAGTTGATCAATGAATTCGAGATCGTTAAGGAGGATGACGTCCCTGCTGCAGATCAGGATATCGAGCCAGATCAGGGTGATTCTGCTGCTGCTGCTGCCCCTGAACCAGCTGCAAGGACAGACCAAGGTAGTGCCCCGGAGCAAGGCGTGGCACCAATGGACATCTAA

Protein sequence

MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQKKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFKDSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKEDDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI
Homology
BLAST of CmaCh12G004550 vs. ExPASy Swiss-Prot
Match: O23712 (Proteasome subunit alpha type-1-B OS=Arabidopsis thaliana OX=3702 GN=PAF2 PE=1 SV=2)

HSP 1 Score: 443.7 bits (1140), Expect = 1.5e-123
Identity = 220/284 (77.46%), Postives = 252/284 (88.73%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS++HVVLACVNKA SELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           +KIFKVDDHIGVAI+GLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVV LADKAQVCT
Sbjct: 61  RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLER+FE+F+
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERKFESFQ 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           +SS++DLIKDA++A RETLQGE L SS+CT+++LGV EPFH LD+E++QK+I+ FE V E
Sbjct: 181 ESSKEDLIKDAIMAIRETLQGETLKSSLCTVSVLGVDEPFHFLDQESIQKVIDTFEKVPE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           ++  A + + EP+       AAP  A   +QG + +Q VAPM+I
Sbjct: 241 EEEDAGEGEAEPE-------AAPGAAGTGEQGGSGDQDVAPMEI 277

BLAST of CmaCh12G004550 vs. ExPASy Swiss-Prot
Match: P34066 (Proteasome subunit alpha type-1-A OS=Arabidopsis thaliana OX=3702 GN=PAF1 PE=1 SV=3)

HSP 1 Score: 439.9 bits (1130), Expect = 2.2e-122
Identity = 223/284 (78.52%), Postives = 247/284 (86.97%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS++HVVLACVNKA SELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           +KIFKVDDHIGVAI+GLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVV LADKAQVCT
Sbjct: 61  RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFE+F 
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFESFG 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           DSSR+DLIKDA++A RETLQGE L SS+CT+AILGV EPFH LD+E +QK+I+ FE V E
Sbjct: 181 DSSREDLIKDAILAVRETLQGETLKSSLCTVAILGVDEPFHFLDQEAIQKVIDTFEKVPE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
                 +++ E + G+  A AA    A    G A +Q VAPM++
Sbjct: 241 ------EEEGEGEAGEGEAEAAEAAPAERGGGVAGDQDVAPMEM 278

BLAST of CmaCh12G004550 vs. ExPASy Swiss-Prot
Match: P52428 (Proteasome subunit alpha type-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PAF1 PE=2 SV=1)

HSP 1 Score: 379.0 bits (972), Expect = 4.6e-104
Identity = 189/268 (70.52%), Postives = 223/268 (83.21%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSA +GLRS+TH VLA  NKA SELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSACVGLRSRTHAVLAAANKAASELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           +K+F+V DH GVA++GLTADGRVLSR++RSECIN+++ Y++PLPV RL ++LADKAQVCT
Sbjct: 61  RKVFRVADHAGVALAGLTADGRVLSRFLRSECINHAFVYDAPLPVSRLALRLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLV GLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKT+LERRFE + 
Sbjct: 121 QRSWKRPYGVGLLVAGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTFLERRFEGYN 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAIL-----GVGEPFHILDRETVQKLINEF 240
           D + + LIKDAL A +ETLQGEKLTSS CT+AI+     G  EPF ++D + +Q++I+  
Sbjct: 181 DYTPEQLIKDALSAIKETLQGEKLTSSNCTVAIVGRKDDGTVEPFEMIDVKRIQEIIDSM 240

Query: 241 EIVKEDDVPAADQDI----EPDQGDSAA 260
           E  +E   PAA+ +     E D+G  AA
Sbjct: 241 EAAEE--APAAEAESSSMQEEDKGTDAA 266

BLAST of CmaCh12G004550 vs. ExPASy Swiss-Prot
Match: Q27562 (Proteasome subunit alpha type-1 OS=Dictyostelium discoideum OX=44689 GN=psmA1 PE=3 SV=1)

HSP 1 Score: 278.1 bits (710), Expect = 1.1e-73
Identity = 133/238 (55.88%), Postives = 179/238 (75.21%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           M+RNQYD+D+TT+SP+GR+ Q+EYAMEAVKQG AA+ L+SK   VL  + +++SEL S+Q
Sbjct: 1   MYRNQYDSDITTFSPSGRIHQIEYAMEAVKQGGAAVALKSKQFAVLVSLKRSSSELGSYQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIF VDDH+G+AISGLTAD R+L  YMR+EC NY+Y YES + V RLV ++ADK QV T
Sbjct: 61  KKIFVVDDHVGIAISGLTADARMLCNYMRNECSNYNYVYESQMRVERLVTKVADKHQVHT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QR  +RPYGVGLLV G D+ GAH+Y  CPSGN+++Y++ +IGSRSQ+AKTYLE+ FE+F+
Sbjct: 121 QRYGRRPYGVGLLVAGYDQLGAHIYQTCPSGNFYDYKSISIGSRSQSAKTYLEKHFESFE 180

Query: 181 DSSRDDLIKDALIATRETLQG--EKLTSSICTIAILGVGEPFHILDRETVQKLINEFE 237
           + + + LI   L A RET+    + L S   +I I+G G+PF I++    Q  +N  E
Sbjct: 181 ECTLEQLITHGLRALRETIPNTDDTLNSKNVSIGIVGEGQPFKIIEEADAQPYLNLLE 238

BLAST of CmaCh12G004550 vs. ExPASy Swiss-Prot
Match: Q9R1P4 (Proteasome subunit alpha type-1 OS=Mus musculus OX=10090 GN=Psma1 PE=1 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 8.2e-69
Identity = 132/272 (48.53%), Postives = 179/272 (65.81%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYD DVT WSP GR+ Q+EYAMEAVKQGSA +GL+SKTH VL  + +A SEL++HQ
Sbjct: 1   MFRNQYDNDVTVWSPQGRIHQIEYAMEAVKQGSATVGLKSKTHAVLVALKRAQSELAAHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKI  VD+HIG++I+GLTAD R+L  +MR EC++  + ++ PLPV RLV  +  K Q+ T
Sbjct: 61  KKILHVDNHIGISIAGLTADARLLCNFMRQECLDSRFVFDRPLPVSRLVSLIGSKTQIPT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QR  +RPYGVGLL+ G D+ G H++  CPS NYF+ +A +IG+RSQ+A+TYLER    F 
Sbjct: 121 QRYGRRPYGVGLLIAGYDDMGPHIFQTCPSANYFDCRAMSIGARSQSARTYLERHMSEFM 180

Query: 181 DSSRDDLIKDALIATRETLQGEK-LTSSICTIAILGVGEPFHILDRETVQKLINEFEIVK 240
           + + D+L+K  L A RETL  E+ LT+   +I I+G    F I D + V   ++  E   
Sbjct: 181 ECNLDELVKHGLRALRETLPAEQDLTTKNVSIGIVGKDLEFTIYDDDDVSPFLDGLE--- 240

Query: 241 EDDVPAADQDIEPDQGDSAAAAAPEPAARTDQ 272
                       P +    + AA EPA + D+
Sbjct: 241 ----------ERPQRKAQPSQAAEEPAEKADE 259

BLAST of CmaCh12G004550 vs. ExPASy TrEMBL
Match: A0A6J1KQI9 (Proteasome subunit alpha type OS=Cucurbita maxima OX=3661 GN=LOC111496294 PE=3 SV=1)

HSP 1 Score: 563.1 bits (1450), Expect = 6.4e-157
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE
Sbjct: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI
Sbjct: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 284

BLAST of CmaCh12G004550 vs. ExPASy TrEMBL
Match: A0A6J1GIC0 (Proteasome subunit alpha type OS=Cucurbita moschata OX=3662 GN=LOC111454419 PE=3 SV=1)

HSP 1 Score: 553.5 bits (1425), Expect = 5.1e-154
Identity = 281/284 (98.94%), Postives = 282/284 (99.30%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           +SSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE
Sbjct: 181 NSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           DDVPAADQDIEPDQGDS  AAAPEPAARTDQGSAPEQGVAPMDI
Sbjct: 241 DDVPAADQDIEPDQGDS--AAAPEPAARTDQGSAPEQGVAPMDI 282

BLAST of CmaCh12G004550 vs. ExPASy TrEMBL
Match: A0A5A7VC66 (Proteasome subunit alpha type OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G005560 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 3.4e-150
Identity = 274/284 (96.48%), Postives = 278/284 (97.89%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRS THVVLACVNKANSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           DSSRD+LIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLI+EFEIVKE
Sbjct: 181 DSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLIDEFEIVKE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           DDVPAADQDI P+QGDS   AAPEPAA TDQGSAP+QGVAPMDI
Sbjct: 241 DDVPAADQDIAPEQGDS---AAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of CmaCh12G004550 vs. ExPASy TrEMBL
Match: A0A1S3BBW8 (Proteasome subunit alpha type OS=Cucumis melo OX=3656 GN=LOC103487987 PE=3 SV=1)

HSP 1 Score: 540.8 bits (1392), Expect = 3.4e-150
Identity = 274/284 (96.48%), Postives = 278/284 (97.89%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRS THVVLACVNKANSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           DSSRD+LIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLI+EFEIVKE
Sbjct: 181 DSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLIDEFEIVKE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           DDVPAADQDI P+QGDS   AAPEPAA TDQGSAP+QGVAPMDI
Sbjct: 241 DDVPAADQDIAPEQGDS---AAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of CmaCh12G004550 vs. ExPASy TrEMBL
Match: A0A0A0LLH5 (Proteasome subunit alpha type OS=Cucumis sativus OX=3659 GN=Csa_2G361820 PE=3 SV=1)

HSP 1 Score: 539.3 bits (1388), Expect = 9.9e-150
Identity = 273/284 (96.13%), Postives = 277/284 (97.54%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRS THVVLACVNKANSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           DSSRD+LIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLI+EFEIVKE
Sbjct: 181 DSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLIDEFEIVKE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           DDVPA DQDI P+QGDS   AAPEPAA TDQGSAP+QGVAPMDI
Sbjct: 241 DDVPAVDQDIAPEQGDS---AAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of CmaCh12G004550 vs. NCBI nr
Match: XP_023002464.1 (proteasome subunit alpha type-1-B-like [Cucurbita maxima])

HSP 1 Score: 563.1 bits (1450), Expect = 1.3e-156
Identity = 284/284 (100.00%), Postives = 284/284 (100.00%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE
Sbjct: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI
Sbjct: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 284

BLAST of CmaCh12G004550 vs. NCBI nr
Match: XP_022951677.1 (proteasome subunit alpha type-1-B-like [Cucurbita moschata] >XP_023538349.1 proteasome subunit alpha type-1-B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 553.5 bits (1425), Expect = 1.0e-153
Identity = 281/284 (98.94%), Postives = 282/284 (99.30%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           +SSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE
Sbjct: 181 NSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           DDVPAADQDIEPDQGDS  AAAPEPAARTDQGSAPEQGVAPMDI
Sbjct: 241 DDVPAADQDIEPDQGDS--AAAPEPAARTDQGSAPEQGVAPMDI 282

BLAST of CmaCh12G004550 vs. NCBI nr
Match: KAG6585582.1 (Proteasome subunit alpha type-1-B, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 552.0 bits (1421), Expect = 3.0e-153
Identity = 280/284 (98.59%), Postives = 281/284 (98.94%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           +SSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE
Sbjct: 181 NSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           DD PAADQDIEPDQGDS  AAAPEPAARTDQGSAPEQGVAPMDI
Sbjct: 241 DDAPAADQDIEPDQGDS--AAAPEPAARTDQGSAPEQGVAPMDI 282

BLAST of CmaCh12G004550 vs. NCBI nr
Match: XP_008444739.1 (PREDICTED: proteasome subunit alpha type-1-B-like [Cucumis melo] >KAA0065218.1 proteasome subunit alpha type-1-B-like [Cucumis melo var. makuwa])

HSP 1 Score: 540.8 bits (1392), Expect = 7.0e-150
Identity = 274/284 (96.48%), Postives = 278/284 (97.89%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRS THVVLACVNKANSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSNTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           DSSRD+LIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLI+EFEIVKE
Sbjct: 181 DSSRDELIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLIDEFEIVKE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           DDVPAADQDI P+QGDS   AAPEPAA TDQGSAP+QGVAPMDI
Sbjct: 241 DDVPAADQDIAPEQGDS---AAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of CmaCh12G004550 vs. NCBI nr
Match: XP_023546840.1 (proteasome subunit alpha type-1-B-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 540.0 bits (1390), Expect = 1.2e-149
Identity = 272/284 (95.77%), Postives = 279/284 (98.24%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT
Sbjct: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           DSSRDDLIKDAL+ATRETLQGEKLTSSICTI+ILGVGEPFHILDRETVQ+LI+EFEIVKE
Sbjct: 181 DSSRDDLIKDALMATRETLQGEKLTSSICTISILGVGEPFHILDRETVQQLIDEFEIVKE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           DDVPAADQD+ P+QGDS   AAPEPAA TDQGSAP+QGVAPMDI
Sbjct: 241 DDVPAADQDVAPEQGDS---AAPEPAAGTDQGSAPDQGVAPMDI 281

BLAST of CmaCh12G004550 vs. TAIR 10
Match: AT1G47250.1 (20S proteasome alpha subunit F2 )

HSP 1 Score: 443.7 bits (1140), Expect = 1.1e-124
Identity = 220/284 (77.46%), Postives = 252/284 (88.73%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS++HVVLACVNKA SELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           +KIFKVDDHIGVAI+GLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVV LADKAQVCT
Sbjct: 61  RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLER+FE+F+
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERKFESFQ 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           +SS++DLIKDA++A RETLQGE L SS+CT+++LGV EPFH LD+E++QK+I+ FE V E
Sbjct: 181 ESSKEDLIKDAIMAIRETLQGETLKSSLCTVSVLGVDEPFHFLDQESIQKVIDTFEKVPE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
           ++  A + + EP+       AAP  A   +QG + +Q VAPM+I
Sbjct: 241 EEEDAGEGEAEPE-------AAPGAAGTGEQGGSGDQDVAPMEI 277

BLAST of CmaCh12G004550 vs. TAIR 10
Match: AT5G42790.1 (proteasome alpha subunit F1 )

HSP 1 Score: 439.9 bits (1130), Expect = 1.6e-123
Identity = 223/284 (78.52%), Postives = 247/284 (86.97%), Query Frame = 0

Query: 1   MFRNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSELSSHQ 60
           MFRNQYDTDVTTWSP GRLFQVEYAMEAVKQGSAAIGLRS++HVVLACVNKA SELSSHQ
Sbjct: 1   MFRNQYDTDVTTWSPTGRLFQVEYAMEAVKQGSAAIGLRSRSHVVLACVNKAQSELSSHQ 60

Query: 61  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKAQVCT 120
           +KIFKVDDHIGVAI+GLTADGRVLSRYMRSE IN+S+TYESPLPVGRLVV LADKAQVCT
Sbjct: 61  RKIFKVDDHIGVAIAGLTADGRVLSRYMRSESINHSFTYESPLPVGRLVVHLADKAQVCT 120

Query: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFENFK 180
           QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFE+F 
Sbjct: 121 QRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFESFG 180

Query: 181 DSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLINEFEIVKE 240
           DSSR+DLIKDA++A RETLQGE L SS+CT+AILGV EPFH LD+E +QK+I+ FE V E
Sbjct: 181 DSSREDLIKDAILAVRETLQGETLKSSLCTVAILGVDEPFHFLDQEAIQKVIDTFEKVPE 240

Query: 241 DDVPAADQDIEPDQGDSAAAAAPEPAARTDQGSAPEQGVAPMDI 285
                 +++ E + G+  A AA    A    G A +Q VAPM++
Sbjct: 241 ------EEEGEGEAGEGEAEAAEAAPAERGGGVAGDQDVAPMEM 278

BLAST of CmaCh12G004550 vs. TAIR 10
Match: AT1G53850.1 (20S proteasome alpha subunit E1 )

HSP 1 Score: 155.2 bits (391), Expect = 7.7e-38
Identity = 94/235 (40.00%), Postives = 136/235 (57.87%), Query Frame = 0

Query: 3   RNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSEL--SSHQ 62
           R +YD  V T+SP GRLFQVEYA+EA+K GS AIG+++K  VVLA   +  S L   S  
Sbjct: 5   RTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLEPSSV 64

Query: 63  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---Q 122
           +KI ++DDHIG A+SGL AD R L  + R E  N+ ++Y  P+ V      L D A    
Sbjct: 65  EKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRFG 124

Query: 123 VCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFE 182
              + S  RP+GV LL+ G DE+G  LYY  PSG +++  A AIGS S+ A + L+ +F 
Sbjct: 125 EGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFN 184

Query: 183 NFKDSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLI 233
             KD S  +    A+   ++ ++ EK+T +   + I  V   +H+   + V+ +I
Sbjct: 185 --KDLSLQEAETIAVSILKQVME-EKVTPN--NVDIAKVAPAYHLYTPQEVEAVI 234

BLAST of CmaCh12G004550 vs. TAIR 10
Match: AT1G53850.2 (20S proteasome alpha subunit E1 )

HSP 1 Score: 155.2 bits (391), Expect = 7.7e-38
Identity = 94/235 (40.00%), Postives = 136/235 (57.87%), Query Frame = 0

Query: 3   RNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSEL--SSHQ 62
           R +YD  V T+SP GRLFQVEYA+EA+K GS AIG+++K  VVLA   +  S L   S  
Sbjct: 5   RTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLEPSSV 64

Query: 63  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---Q 122
           +KI ++DDHIG A+SGL AD R L  + R E  N+ ++Y  P+ V      L D A    
Sbjct: 65  EKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRFG 124

Query: 123 VCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFE 182
              + S  RP+GV LL+ G DE+G  LYY  PSG +++  A AIGS S+ A + L+ +F 
Sbjct: 125 EGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFN 184

Query: 183 NFKDSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLI 233
             KD S  +    A+   ++ ++ EK+T +   + I  V   +H+   + V+ +I
Sbjct: 185 --KDLSLQEAETIAVSILKQVME-EKVTPN--NVDIAKVAPAYHLYTPQEVEAVI 234

BLAST of CmaCh12G004550 vs. TAIR 10
Match: AT3G14290.1 (20S proteasome alpha subunit E2 )

HSP 1 Score: 154.5 bits (389), Expect = 1.3e-37
Identity = 93/236 (39.41%), Postives = 137/236 (58.05%), Query Frame = 0

Query: 3   RNQYDTDVTTWSPAGRLFQVEYAMEAVKQGSAAIGLRSKTHVVLACVNKANSEL--SSHQ 62
           R +YD  V T+SP GRLFQVEYA+EA+K GS AIG+++K  VVLA   +  S L   S  
Sbjct: 5   RTEYDRGVNTFSPEGRLFQVEYAIEAIKLGSTAIGVKTKEGVVLAVEKRITSPLLEPSSV 64

Query: 63  KKIFKVDDHIGVAISGLTADGRVLSRYMRSECINYSYTYESPLPVGRLVVQLADKA---Q 122
           +KI ++DDHIG A+SGL AD R L  + R E  N+ ++Y  P+ V      L D A    
Sbjct: 65  EKIMEIDDHIGCAMSGLIADARTLVEHARVETQNHRFSYGEPMTVESTTQALCDLALRFG 124

Query: 123 VCTQRSWKRPYGVGLLVGGLDESGAHLYYNCPSGNYFEYQAFAIGSRSQAAKTYLERRFE 182
              + S  RP+GV LL+ G DE+G  LYY  PSG +++  A AIGS S+ A + L+ +F 
Sbjct: 125 EGEEESMSRPFGVSLLIAGHDENGPSLYYTDPSGTFWQCNAKAIGSGSEGADSSLQEQFN 184

Query: 183 NFKDSSRDDLIKDALIATRETLQGEKLTSSICTIAILGVGEPFHILDRETVQKLIN 234
             KD +  +    A+   ++ ++ EK+T +   + I  V   +H+   + V+ +I+
Sbjct: 185 --KDITLQEAETIAVSILKQVME-EKVTPN--NVDIAKVAPAYHLYTPQEVEAVIS 235

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O237121.5e-12377.46Proteasome subunit alpha type-1-B OS=Arabidopsis thaliana OX=3702 GN=PAF2 PE=1 S... [more]
P340662.2e-12278.52Proteasome subunit alpha type-1-A OS=Arabidopsis thaliana OX=3702 GN=PAF1 PE=1 S... [more]
P524284.6e-10470.52Proteasome subunit alpha type-1 OS=Oryza sativa subsp. japonica OX=39947 GN=PAF1... [more]
Q275621.1e-7355.88Proteasome subunit alpha type-1 OS=Dictyostelium discoideum OX=44689 GN=psmA1 PE... [more]
Q9R1P48.2e-6948.53Proteasome subunit alpha type-1 OS=Mus musculus OX=10090 GN=Psma1 PE=1 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1KQI96.4e-157100.00Proteasome subunit alpha type OS=Cucurbita maxima OX=3661 GN=LOC111496294 PE=3 S... [more]
A0A6J1GIC05.1e-15498.94Proteasome subunit alpha type OS=Cucurbita moschata OX=3662 GN=LOC111454419 PE=3... [more]
A0A5A7VC663.4e-15096.48Proteasome subunit alpha type OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_sc... [more]
A0A1S3BBW83.4e-15096.48Proteasome subunit alpha type OS=Cucumis melo OX=3656 GN=LOC103487987 PE=3 SV=1[more]
A0A0A0LLH59.9e-15096.13Proteasome subunit alpha type OS=Cucumis sativus OX=3659 GN=Csa_2G361820 PE=3 SV... [more]
Match NameE-valueIdentityDescription
XP_023002464.11.3e-156100.00proteasome subunit alpha type-1-B-like [Cucurbita maxima][more]
XP_022951677.11.0e-15398.94proteasome subunit alpha type-1-B-like [Cucurbita moschata] >XP_023538349.1 prot... [more]
KAG6585582.13.0e-15398.59Proteasome subunit alpha type-1-B, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_008444739.17.0e-15096.48PREDICTED: proteasome subunit alpha type-1-B-like [Cucumis melo] >KAA0065218.1 p... [more]
XP_023546840.11.2e-14995.77proteasome subunit alpha type-1-B-like [Cucurbita pepo subsp. pepo][more]
Match NameE-valueIdentityDescription
AT1G47250.11.1e-12477.4620S proteasome alpha subunit F2 [more]
AT5G42790.11.6e-12378.52proteasome alpha subunit F1 [more]
AT1G53850.17.7e-3840.0020S proteasome alpha subunit E1 [more]
AT1G53850.27.7e-3840.0020S proteasome alpha subunit E1 [more]
AT3G14290.11.3e-3739.4120S proteasome alpha subunit E2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000426Proteasome alpha-subunit, N-terminal domainSMARTSM00948Proteasome_A_N_2coord: 6..28
e-value: 4.5E-10
score: 49.5
IPR000426Proteasome alpha-subunit, N-terminal domainPFAMPF10584Proteasome_A_Ncoord: 6..28
e-value: 6.9E-14
score: 51.2
IPR000426Proteasome alpha-subunit, N-terminal domainPROSITEPS00388PROTEASOME_ALPHA_1coord: 6..28
IPR001353Proteasome, subunit alpha/betaPFAMPF00227Proteasomecoord: 29..213
e-value: 1.8E-57
score: 193.9
IPR029055Nucleophile aminohydrolases, N-terminalGENE3D3.60.20.10Glutamine Phosphoribosylpyrophosphate, subunit 1, domain 1coord: 1..236
e-value: 1.0E-84
score: 285.5
IPR029055Nucleophile aminohydrolases, N-terminalSUPERFAMILY56235N-terminal nucleophile aminohydrolases (Ntn hydrolases)coord: 4..235
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 242..284
NoneNo IPR availablePANTHERPTHR11599PROTEASOME SUBUNIT ALPHA/BETAcoord: 1..275
NoneNo IPR availablePANTHERPTHR11599:SF182PROTEASOME SUBUNIT ALPHA TYPEcoord: 1..275
IPR023332Proteasome alpha-type subunitPROSITEPS51475PROTEASOME_ALPHA_2coord: 21..232
score: 76.424675
IPR035144Proteasome subunit alpha 1CDDcd03749proteasome_alpha_type_1coord: 6..215
e-value: 9.93004E-146
score: 405.521

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G004550.1CmaCh12G004550.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010498 proteasomal protein catabolic process
biological_process GO:0006511 ubiquitin-dependent protein catabolic process
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
cellular_component GO:0005737 cytoplasm
cellular_component GO:0005634 nucleus
cellular_component GO:0019773 proteasome core complex, alpha-subunit complex
cellular_component GO:0005839 proteasome core complex