CmaCh12G003220 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G003220
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptiontRNA (Cytosine(38)-C(5))-methyltransferase
LocationCma_Chr12: 1653641 .. 1655427 (-)
RNA-Seq ExpressionCmaCh12G003220
SyntenyCmaCh12G003220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCTTTGTTTTACAAAAAGCGTCAACCGATACGTGAACGGCATTGGCTTGCTTTTGGCACCTTTTAAGGTAAAATTTTATCCTTAGCCATGATTTTTTTTACTATGTGGTGGTGGTGCAATAGAGAGAATTTTTCCTGAACGTGGAAATGAAGCATGATCCAAACTTGATGCTGTATGTTAAGTAGTAAACATCAATCGTCTATTGTTATCATATTTAGATTTCATTTTTGGTTAAAATTTGATCTAACATGGTGATTTCAATTCCCCCCCTCTTCTCGTGTTGGGCAGCAGTGATGTTCTAGGATTATAACACATGCCGCTTTTCTGGCACCATTATTGAACTCTTGTCACACTGACTGGTAACGCAATGCAGGCGGAAATGGTGGATAAGGCTCATTCTCTGAAAGAGGCGCGACGCTCAGATATTGTACTCCTCTAGAGAGGTGCATGATGATTTTATATTAAGATAGTTCTTGATTATATCTCATTTTCAAGCTGATGACGTTCATTAATCCGTAGCTTTTTTTAATGAAAAATACTAGGTTGCTAACTTGCATTTCTTTCCGGAAGATTTTCAATTTCCACAGCATATTGGCCCTGGGCAACGGTATGAATCTTCGTTCTTTTAACAAAATTTCTTTAATGTACTTTTCACGTTTGAATTCCTAACTAAACTAAAGAAATAGAAACAAAAAGTTGAAAAATATTTATTTAAAAAAATTCAAAACTAACCTTTCATCTCGTATTCATTTGGAACTAATAAATTCAACTAGATATTAAACACAAGATAAGTAAATGGAGAATAAAAAAAAATGAAAAGAAAAAAGGATTCTAGAAGGGATTATTCAACTTCCTAATCTTCGCTACCAGAAAAATACTCATTTTCATAGCCTTTGATGCCAGAAAAATTCTAAATTCCTTTTCTTGTTTTGAAATAATAAAATAATTATTTATTAAAAAATATTCATCTTCCTATCCTTCGGAACCCAGAAAATTTTCAAATTTCTTTTCTTTTCTTGTTCTTATTTTGAAATAATAAAATAATTAGTTATTCTTGGTTCCATTCGTCAAAACATTTCAATTTTTGTTAGATCATTAAATTCAGTTTGAAAATTAAGAACACTTTAAAGAAGGTAGAATTTACAAGTTTAATTTTCAAAAACTAAATTACAAGTTTAATTAAACCAGATAGATATCTGTAGCCGATTGATACCTTGCCCTGCCTATAAGCAGTTTTGAGATTTTATTGATGATTCTCGCCAACTGTTTCAACTGTTCAAAATTGCCACATACATAATAGAAAAATGTTTAATATCACCTTTTGCCTTGGGTTTTTCTTTGCAGCCATGCATTGCTAGGGAACAGTTTACGCATAGCCGTTGCTGCTCCCTTGCTCCGATACCTCTTTGCAGAGACATCTGGATTTACATGCTCTCTTTTCTAAAGCCAAGACCACATCAATTGGGACAGACCATGAACTAGCATTGTTCTCTTTAGATATTTTGCCCTTAAATGTAGCATCAAATTTTCTACTAAATTATCAAAACCATTTTAGCTGTCGGAGAGTAATTTGTTTTACCTATTTAGTTGACAAGACAAAAAGTGCTAATGGTATTCATTTATCAAAATTTAATCGATGGCAGTAGTAATATATCAAACCAAACCTACTATGTCCAATTATATAGTGAATTACACAACCAACAACCGACCTCAATAAAGAACCACATAGGTCGGCCTTCCAAACTCCAGAAAACCACAACCACAAACCACACCATAAAACCCTAA

mRNA sequence

ATGCTTTGTTTTACAAAAAGCGTCAACCGATACGTGAACGGCATTGGCTTGCTTTTGGCACCTTTTAAGGCGGAAATGGTGGATAAGGCTCATTCTCTGAAAGAGGCGCGACGCTCAGATATTGTTGCTAACTTGCATTTCTTTCCGGAAGATTTTCAATTTCCACAGCATATTGGCCCTGGGCAACGCCATGCATTGCTAGGGAACAGTTTACGCATAGCCTTGACAAGACAAAAAGTGCTAATGGTATTCATTTATCAAAATTTAATCGATGGCAGTAGTAATATATCAAACCAAACCTACTATGTCCAATTATATAGTGAATTACACAACCAACAACCGACCTCAATAAAGAACCACATAGGTCGGCCTTCCAAACTCCAGAAAACCACAACCACAAACCACACCATAAAACCCTAA

Coding sequence (CDS)

ATGCTTTGTTTTACAAAAAGCGTCAACCGATACGTGAACGGCATTGGCTTGCTTTTGGCACCTTTTAAGGCGGAAATGGTGGATAAGGCTCATTCTCTGAAAGAGGCGCGACGCTCAGATATTGTTGCTAACTTGCATTTCTTTCCGGAAGATTTTCAATTTCCACAGCATATTGGCCCTGGGCAACGCCATGCATTGCTAGGGAACAGTTTACGCATAGCCTTGACAAGACAAAAAGTGCTAATGGTATTCATTTATCAAAATTTAATCGATGGCAGTAGTAATATATCAAACCAAACCTACTATGTCCAATTATATAGTGAATTACACAACCAACAACCGACCTCAATAAAGAACCACATAGGTCGGCCTTCCAAACTCCAGAAAACCACAACCACAAACCACACCATAAAACCCTAA

Protein sequence

MLCFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDIVANLHFFPEDFQFPQHIGPGQRHALLGNSLRIALTRQKVLMVFIYQNLIDGSSNISNQTYYVQLYSELHNQQPTSIKNHIGRPSKLQKTTTTNHTIKP
Homology
[Close]
BLAST of CmaCh12G003220 vs. ExPASy Swiss-Prot
Match: F4JWT7 (tRNA (cytosine(38)-C(5))-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=DNMT2 PE=1 SV=1)

HSP 1 Score: 77.8 bits (190), Expect = 1.1e-13
Identity = 41/86 (47.67%), Postives = 52/86 (60.47%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLA  + ++  K   LKE R        VAN H FPEDF+FP+HI
Sbjct: 296 CFTKSYYRYVKGTGSLLATVQPKIKGKESCLKEQRLRYFTPREVANFHSFPEDFEFPKHI 355

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
              QR+A+LGNSL +A+    +  +F
Sbjct: 356 SLRQRYAMLGNSLSVAVVAPLLRYLF 381

[Close]
BLAST of CmaCh12G003220 vs. ExPASy Swiss-Prot
Match: O14717 (tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=1 SV=1)

HSP 1 Score: 45.8 bits (107), Expect = 4.5e-04
Identity = 28/100 (28.00%), Postives = 47/100 (47.00%), Query Frame = 0 

Query: 2   LCFTKSVNRYVNGIGLLLAPFKAEMVDKAH-SLKEARRSD----------------IVAN 61
           +CFTK    Y+ G G +L   +   V+  + SL    + +                 +AN
Sbjct: 291 VCFTKGYGSYIEGTGSVLQTAEDVQVENIYKSLTNLSQEEQITKLLILKLRYFTPKEIAN 350

Query: 62  LHFFPEDFQFPQHIGPGQRHALLGNSLRIALTRQKVLMVF 85
           L  FP +F FP+ I   QR+ LLGNSL + +  + + +++
Sbjct: 351 LLGFPPEFGFPEKITVKQRYRLLGNSLNVHVVAKLIKILY 390

[Close]
BLAST of CmaCh12G003220 vs. ExPASy TrEMBL
Match: A0A1S3BC07 (tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN=LOC103488282 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.3e-17
Identity = 51/86 (59.30%), Postives = 60/86 (69.77%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLAPF+AE + KAHSLKE +        VANLH FP+DFQFPQHI
Sbjct: 296 CFTKSYYRYVKGTGSLLAPFEAERMGKAHSLKEQKLRYFTPREVANLHSFPKDFQFPQHI 355

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
           G  QR+ALLGNSL +A+    +  +F
Sbjct: 356 GLRQRYALLGNSLSVAVVAPLLRYLF 381

[Close]
BLAST of CmaCh12G003220 vs. ExPASy TrEMBL
Match: A0A1S3BC14 (tRNA (Cytosine(38)-C(5))-methyltransferase isoform X2 OS=Cucumis melo OX=3656 GN=LOC103488282 PE=3 SV=1)

HSP 1 Score: 99.4 bits (246), Expect = 1.3e-17
Identity = 51/86 (59.30%), Postives = 60/86 (69.77%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLAPF+AE + KAHSLKE +        VANLH FP+DFQFPQHI
Sbjct: 249 CFTKSYYRYVKGTGSLLAPFEAERMGKAHSLKEQKLRYFTPREVANLHSFPKDFQFPQHI 308

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
           G  QR+ALLGNSL +A+    +  +F
Sbjct: 309 GLRQRYALLGNSLSVAVVAPLLRYLF 334

[Close]
BLAST of CmaCh12G003220 vs. ExPASy TrEMBL
Match: A0A0A0LSE8 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380010 PE=3 SV=1)

HSP 1 Score: 99.0 bits (245), Expect = 1.7e-17
Identity = 52/86 (60.47%), Postives = 59/86 (68.60%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLAPF+ E + KAHSLKE +        VANLH FPEDFQFPQHI
Sbjct: 296 CFTKSYYRYVKGTGSLLAPFETEGMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHI 355

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
           G  QR+ALLGNSL IA+    +  +F
Sbjct: 356 GLRQRYALLGNSLSIAVVAPLLRYLF 381

[Close]
BLAST of CmaCh12G003220 vs. ExPASy TrEMBL
Match: A0A6J1HD73 (tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111462432 PE=3 SV=1)

HSP 1 Score: 98.6 bits (244), Expect = 2.2e-17
Identity = 51/86 (59.30%), Postives = 59/86 (68.60%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLAPF+ E + KAHSLKE +        VANLH FPEDF+FPQHI
Sbjct: 297 CFTKSYYRYVKGTGSLLAPFEVERMGKAHSLKEQKLRYFTPREVANLHSFPEDFEFPQHI 356

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
           G  QR+ALLGNSL IA+    +  +F
Sbjct: 357 GLRQRYALLGNSLSIAVVAPLLKYLF 382

[Close]
BLAST of CmaCh12G003220 vs. ExPASy TrEMBL
Match: A0A6J1K9S0 (tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111491952 PE=3 SV=1)

HSP 1 Score: 97.1 bits (240), Expect = 6.4e-17
Identity = 50/86 (58.14%), Postives = 59/86 (68.60%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLAPF+ + + KAHSLKE +        VANLH FPEDF+FPQHI
Sbjct: 298 CFTKSYYRYVKGTGSLLAPFEVKRMGKAHSLKEQKLRYFTPREVANLHSFPEDFEFPQHI 357

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
           G  QR+ALLGNSL IA+    +  +F
Sbjct: 358 GLRQRYALLGNSLSIAVVAPLLKYLF 383

[Close]
BLAST of CmaCh12G003220 vs. NCBI nr
Match: XP_038884641.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X2 [Benincasa hispida])

HSP 1 Score: 100.1 bits (248), Expect = 1.5e-17
Identity = 52/86 (60.47%), Postives = 60/86 (69.77%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLAPF+AE + KAHSLKE +        VANLH FPEDFQFP+HI
Sbjct: 235 CFTKSYYRYVKGTGSLLAPFEAERMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPEHI 294

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
           G  QR+ALLGNSL IA+    +  +F
Sbjct: 295 GLRQRYALLGNSLSIAVVAPLLRYLF 320

[Close]
BLAST of CmaCh12G003220 vs. NCBI nr
Match: XP_038884640.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X1 [Benincasa hispida])

HSP 1 Score: 100.1 bits (248), Expect = 1.5e-17
Identity = 52/86 (60.47%), Postives = 60/86 (69.77%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLAPF+AE + KAHSLKE +        VANLH FPEDFQFP+HI
Sbjct: 297 CFTKSYYRYVKGTGSLLAPFEAERMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPEHI 356

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
           G  QR+ALLGNSL IA+    +  +F
Sbjct: 357 GLRQRYALLGNSLSIAVVAPLLRYLF 382

[Close]
BLAST of CmaCh12G003220 vs. NCBI nr
Match: XP_008445162.1 (PREDICTED: tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo])

HSP 1 Score: 99.4 bits (246), Expect = 2.6e-17
Identity = 51/86 (59.30%), Postives = 60/86 (69.77%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLAPF+AE + KAHSLKE +        VANLH FP+DFQFPQHI
Sbjct: 296 CFTKSYYRYVKGTGSLLAPFEAERMGKAHSLKEQKLRYFTPREVANLHSFPKDFQFPQHI 355

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
           G  QR+ALLGNSL +A+    +  +F
Sbjct: 356 GLRQRYALLGNSLSVAVVAPLLRYLF 381

[Close]
BLAST of CmaCh12G003220 vs. NCBI nr
Match: XP_008445163.1 (PREDICTED: tRNA (cytosine(38)-C(5))-methyltransferase isoform X2 [Cucumis melo])

HSP 1 Score: 99.4 bits (246), Expect = 2.6e-17
Identity = 51/86 (59.30%), Postives = 60/86 (69.77%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLAPF+AE + KAHSLKE +        VANLH FP+DFQFPQHI
Sbjct: 249 CFTKSYYRYVKGTGSLLAPFEAERMGKAHSLKEQKLRYFTPREVANLHSFPKDFQFPQHI 308

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
           G  QR+ALLGNSL +A+    +  +F
Sbjct: 309 GLRQRYALLGNSLSVAVVAPLLRYLF 334

[Close]
BLAST of CmaCh12G003220 vs. NCBI nr
Match: XP_004138735.1 (tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X2 [Cucumis sativus])

HSP 1 Score: 99.0 bits (245), Expect = 3.5e-17
Identity = 52/86 (60.47%), Postives = 59/86 (68.60%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLAPF+ E + KAHSLKE +        VANLH FPEDFQFPQHI
Sbjct: 296 CFTKSYYRYVKGTGSLLAPFETEGMGKAHSLKEQKLRYFTPREVANLHSFPEDFQFPQHI 355

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
           G  QR+ALLGNSL IA+    +  +F
Sbjct: 356 GLRQRYALLGNSLSIAVVAPLLRYLF 381

[Close]
BLAST of CmaCh12G003220 vs. TAIR 10
Match: AT5G25480.1 (DNA methyltransferase-2 )

HSP 1 Score: 77.8 bits (190), Expect = 7.7e-15
Identity = 41/86 (47.67%), Postives = 52/86 (60.47%), Query Frame = 0 

Query: 3   CFTKSVNRYVNGIGLLLAPFKAEMVDKAHSLKEARRSDI----VANLHFFPEDFQFPQHI 62
           CFTKS  RYV G G LLA  + ++  K   LKE R        VAN H FPEDF+FP+HI
Sbjct: 296 CFTKSYYRYVKGTGSLLATVQPKIKGKESCLKEQRLRYFTPREVANFHSFPEDFEFPKHI 355

Query: 63  GPGQRHALLGNSLRIALTRQKVLMVF 85
              QR+A+LGNSL +A+    +  +F
Sbjct: 356 SLRQRYAMLGNSLSVAVVAPLLRYLF 381

The following BLAST results are available for this feature:
BLAST of CmaCh12G003220 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4JWT71.1e-1347.67tRNA (cytosine(38)-C(5))-methyltransferase 2 OS=Arabidopsis thaliana OX=3702 GN=... [more]
O147174.5e-0428.00tRNA (cytosine(38)-C(5))-methyltransferase OS=Homo sapiens OX=9606 GN=TRDMT1 PE=... [more]
BLAST of CmaCh12G003220 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A1S3BC071.3e-1759.30tRNA (Cytosine(38)-C(5))-methyltransferase isoform X1 OS=Cucumis melo OX=3656 GN... [more]
A0A1S3BC141.3e-1759.30tRNA (Cytosine(38)-C(5))-methyltransferase isoform X2 OS=Cucumis melo OX=3656 GN... [more]
A0A0A0LSE81.7e-1760.47Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G380010 PE=3 SV=1[more]
A0A6J1HD732.2e-1759.30tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita moschata OX=3662 GN=LOC1... [more]
A0A6J1K9S06.4e-1758.14tRNA (Cytosine(38)-C(5))-methyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111... [more]
BLAST of CmaCh12G003220 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_038884641.11.5e-1760.47tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X2 [Benincasa hispida][more]
XP_038884640.11.5e-1760.47tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X1 [Benincasa hispida][more]
XP_008445162.12.6e-1759.30PREDICTED: tRNA (cytosine(38)-C(5))-methyltransferase isoform X1 [Cucumis melo][more]
XP_008445163.12.6e-1759.30PREDICTED: tRNA (cytosine(38)-C(5))-methyltransferase isoform X2 [Cucumis melo][more]
XP_004138735.13.5e-1760.47tRNA (cytosine(38)-C(5))-methyltransferase 2 isoform X2 [Cucumis sativus][more]
BLAST of CmaCh12G003220 vs. TAIR 10
Match NameE-valueIdentityDescription
AT5G25480.17.7e-1547.67DNA methyltransferase-2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableGENE3D3.90.120.10DNA Methylase, subunit A, domain 2coord: 1..71
e-value: 7.3E-9
score: 37.4
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 115..139

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G003220.1CmaCh12G003220.1mRNA