CmaCh12G001900 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh12G001900 vs. ExPASy Swiss-Prot
Match: F4JQZ3 (Rho GTPase-activating protein REN1 OS=Arabidopsis thaliana OX=3702 GN=REN1 PE=1 SV=2)

HSP 1 Score: 723.0 bits (1865), Expect = 3.9e-207
Identity = 482/931 (51.77%), Postives = 595/931 (63.91%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPPPPPP-------PP------QDPADGSCA-------------- 60
           M N+NAE     S  PPP   P       PP      Q+P   +C+              
Sbjct: 1   MANKNAE----SSSQPPPHVQPNQQQQQQPPIANEQEQEPHGDTCSIPPAQSGNTDSRSR 60

Query: 61  -GNTVFKSGPLFLSSKGIGWTSWKKRWFILTRTSLVFFRSDPNAVPQKGGEANLTLGGID 120
            GNTVFKSGPL +SSKGIGWTSWKKRWFILTRTSLVFFRSDP+AV QKG E NLTLGGID
Sbjct: 61  GGNTVFKSGPLSISSKGIGWTSWKKRWFILTRTSLVFFRSDPSAVQQKGSEVNLTLGGID 120

Query: 121 LNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEWKAALENALAQAPSSANA- 180
           LNNSGSVVVK++KKLLTVLFPDGR+GRAFTLKA+T+EDL+EWKAALENAL QAPS+++  
Sbjct: 121 LNNSGSVVVKADKKLLTVLFPDGRDGRAFTLKADTMEDLHEWKAALENALTQAPSASHVM 180

Query: 181 --NGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDVDGTPSFLEKALRFIE 240
             NGI +ND  +P  G  E  KD    P +  VLGRP+LLALEDVDG PSFLEKALRF+E
Sbjct: 181 GQNGIFRNDHADPAVGVDEK-KD--ETPTKSTVLGRPVLLALEDVDGAPSFLEKALRFVE 240

Query: 241 THGVKVEGILRQAADVDDVEHRLRGYEQGKIEFSPEEDAHVVGDCVKYVIRELPSSPVPA 300
            HGV++EGILRQAADVDDVEHR+R YE+GK EFSPEEDAH++ DC+KY +RELPSSPVPA
Sbjct: 241 NHGVRIEGILRQAADVDDVEHRIREYEKGKNEFSPEEDAHIIADCLKYFLRELPSSPVPA 300

Query: 301 SCCNALLEASKTERGGRVNAMRSAIYETFPEPNRRLLQRILMMMQAVASHKAENRMSSSA 360
           SCCNALLEA +T+RG RVNAMR+AI E+FPEPNRRLLQRILMMMQ VAS+K  NRM+++A
Sbjct: 301 SCCNALLEACRTDRGNRVNAMRAAICESFPEPNRRLLQRILMMMQTVASNKTVNRMNTNA 360

Query: 361 VAACMAPLLLRPLLAGECEIETDFDVGGDGSIQLLRAAAAANHAQAIVITLLEEYGKIFG 420
           VAACMAPLLLRPLLAG+CEIE DFDVGGDGS+QLL+AAAAANHAQAIVITLLEEY  IFG
Sbjct: 361 VAACMAPLLLRPLLAGDCEIENDFDVGGDGSMQLLQAAAAANHAQAIVITLLEEYESIFG 420

Query: 421 EGSMSPILYSDSEESGSESEEASDDDMSYDDEEQDDATGSDVATDDE--LESAGTCSGSV 480
           EGS+SP LYSDSEESGS +EE SDD+  YDD++ D + GS+  TD+E  LE+    S S 
Sbjct: 421 EGSLSPGLYSDSEESGSGTEEGSDDE-EYDDDD-DGSQGSEDYTDEEEDLENESNGSYSE 480

Query: 481 GSGEHDLY-------DDKGSDVLSTSSKNSDMCDVNGMKPKSSKERIHDKIDRGRGNSPI 540
            +   D Y       D K +D LST SK+             S+     + D G+ +  I
Sbjct: 481 SAASEDKYADSIDPDDHKINDNLSTESKSPKRSKEPKKLLSGSRRSSLPRHDDGKKDEDI 540

Query: 541 --KDEKSCEVEC---ASESFEGSPTTSNKSSNTTKRLTI----------WGRTPAKKKLS 600
             K   + EV+     S S + + +TS+ +S+T K   +          WGRTP KK LS
Sbjct: 541 VVKGVNNTEVKAVVEVSTSEDKNSSTSDVASDTQKPSKLSDAPGGSKRHWGRTPGKKNLS 600

Query: 601 LESIDYDF---GEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLTLE 660
           +ESID+      +  +I+RLE+TK ELQ++I E  + NAVLQ+SLE RKK L  RR  LE
Sbjct: 601 MESIDFSVEVDEDNADIERLESTKLELQSRITEEVKSNAVLQASLERRKKALYGRRQALE 660

Query: 661 QEVARLREQLQREKDPS-----------------ANISEKTKADLGESNRGEKDTANINN 720
           Q+V RL+EQLQ+E+D                     I E  K DL E  + E D A + +
Sbjct: 661 QDVGRLQEQLQQERDRKLALETGLNMSKGNQPIPETIDENLKKDLQEVAQAEADIAKLEH 720

Query: 721 TMDEFVGLLNRIRDQKS-NLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDV 780
            +D+    L     + S +       S+++  H  K+K+K+KD  + +    E S +KD 
Sbjct: 721 KVDDLENRLGHHDGKASGSTHSASKESRKLPEHNAKMKEKQKDTEAASTHISERSTSKD- 780

Query: 781 LSGHAEISKHSPLHQHLDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTK 840
             G     ++    Q       S +     ++   GL+  KR+G + E G    +SAL+K
Sbjct: 781 --GQGAARENETEKQQDSRSKSSQQETSRGSSKLVGLS--KRSGTKGE-GSTTTTSALSK 840

Query: 841 LTTRLNFLKERRSQIANELQNMDRGRVSGQSIDGSEKGRGVE----AQRSVQNSDDTKGS 848
           LT RLNFLKERRSQIANELQNMD+G+  GQ    S + R  E       S Q+ D +K  
Sbjct: 841 LTMRLNFLKERRSQIANELQNMDKGKTLGQPSPTSGQNRVSEETEKGSGSNQDPDSSKLQ 900

BLAST of CmaCh12G001900 vs. ExPASy Swiss-Prot
Match: Q8RWQ4 (Rho GTPase-activating protein 7 OS=Arabidopsis thaliana OX=3702 GN=ROPGAP7 PE=2 SV=1)

HSP 1 Score: 593.2 bits (1528), Expect = 4.7e-168
Identity = 389/842 (46.20%), Postives = 511/842 (60.69%), Query Frame = 0

Query: 33  AGNTVFKSGPLFLSSKGIGWTSWKKRWFILTRTSLVFFRSDPNAVPQKGGEANLTLGGID 92
           A NTVFKSGPLF+SSKG+GWTSWKKRWFILTRTSLVFF++DP  +PQKGGE NLTLGGID
Sbjct: 15  ASNTVFKSGPLFISSKGLGWTSWKKRWFILTRTSLVFFKNDPGTLPQKGGEVNLTLGGID 74

Query: 93  LNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEWKAALENALAQAPSSA--- 152
           LNNSGSVVV+ +KKLLTVLFPDGR+GRAFTLKAET EDLYEWK ALE ALAQAP++A   
Sbjct: 75  LNNSGSVVVREDKKLLTVLFPDGRDGRAFTLKAETFEDLYEWKTALEQALAQAPNAALIM 134

Query: 153 NANGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDVDGTPSFLEKALRFIE 212
             NGI + +       ++E ++  + +P +  V+GRPILLALED+DG+PSFLEKAL+FIE
Sbjct: 135 GQNGIFRAE-------TNEAIEGREKRPLKSLVVGRPILLALEDIDGSPSFLEKALQFIE 194

Query: 213 THGVKVEGILRQAADVDDVEHRLRGYEQGKIEFSPEEDAHVVGDCVKYVIRELPSSPVPA 272
            +G K+EGILRQ+ADV++VE R++ YEQGK EF+ +ED HVVGDC+K+V+RELPSSPV A
Sbjct: 195 KYGTKIEGILRQSADVEEVERRVQEYEQGKTEFTFDEDPHVVGDCIKHVLRELPSSPVSA 254

Query: 273 SCCNALLEASKTE-RGGRVNAMRSAIYETFPEPNRRLLQRILMMMQAVASHKAENRMSSS 332
           SCC ALLEA + E +  R++++RSAI ETFPEPNRRLLQRIL MM  ++SH  ENRM+ +
Sbjct: 255 SCCTALLEAYRIESKEARISSLRSAIAETFPEPNRRLLQRILKMMHTISSHSHENRMNPN 314

Query: 333 AVAACMAPLLLRPLLAGECEIETDFDVGGDGSIQLLRAAAAANHAQAIVITLLEEYGKIF 392
           AVAACMAPLLLRPLLAGEC++E DFD G D S QLL AA AAN+AQAI+  LLE+YG IF
Sbjct: 315 AVAACMAPLLLRPLLAGECDLEDDFDGGEDNSAQLLAAANAANNAQAIITVLLEDYGSIF 374

Query: 393 GEGSMSPI-LYSDSEESGSESEEASDDDMSYDDEEQDDATGSDVATDDELESA--GTCSG 452
            E ++    + ++S    S  +++SDDD +  +   +     +  TDD+ + A  G  S 
Sbjct: 375 DEENIQRCSISTESHIGNSGPDDSSDDDNNMKNGYHNADNEVEPVTDDDNDRALSGKMSE 434

Query: 453 SVGSGEHDLYDDKG-----SDVLSTSSKNSDMCDVNGMKPKSSKERIHDKIDRGR----G 512
           S G    DLY+ KG     SD+ S    N   C+ N       +    +  D+      G
Sbjct: 435 SSGCTGSDLYEYKGFVADDSDIESPRDTNGPRCNSNIRTDHLMRNPFVNSTDQQAGEQIG 494

Query: 513 NSPIKDEKSCEVECASESFEGS------PTTSN-------------------KSSNTTKR 572
           + P K   +  +   SES++ S      PT  N                    SS   KR
Sbjct: 495 DDPTKYGVNSCLAHVSESYQQSGTGLNVPTHGNTLAAPGLESPSAKSVNKGTPSSVHAKR 554

Query: 573 LTIWGRTPAKKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRK 632
            T WGR  A+K  +  S D    +E+ IQRLE TK EL+ +I + AR NA+LQ+SLE RK
Sbjct: 555 ATFWGRGSARKISTDGSFDSSGEDELAIQRLETTKNELRQRIAKEARGNAILQASLERRK 614

Query: 633 KILQERRLTLEQEVARLREQLQREKD----------------PSANISEKTKADLGESNR 692
           + L ERRL+LEQ+V+RL+EQLQ E+D                 S  +  KT+A+L E   
Sbjct: 615 QALHERRLSLEQDVSRLQEQLQAERDLRAALEVGLSMSSGQFSSHGVDSKTRAELEEIAL 674

Query: 693 GEKDTANINNTMDEFVGLLNRIRDQK-SNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAP- 752
            E D A +   + E    LN+ R     +  D R+  Q +QNH  + +  ++D  S    
Sbjct: 675 AEADVARLKQKVAELHHQLNQQRQTHFGSFSDARDTHQYLQNHNPQKRFLQQDFDSTLAY 734

Query: 753 -SHPEHSRNKDVLSG-----------------HAEISKHSPLHQHLDPHNPSAKIGVLAT 788
            +H    R+++ + G                      K  P   +      S + G ++ 
Sbjct: 735 VNHERKQRHEENVLGAEWRNSKGAGSFGVGNSRQPSRKQIPESTNTTDSKISEESGKISV 794

BLAST of CmaCh12G001900 vs. ExPASy Swiss-Prot
Match: Q9FMP8 (Rho GTPase-activating protein 6 OS=Arabidopsis thaliana OX=3702 GN=ROPGAP6 PE=2 SV=1)

HSP 1 Score: 586.6 bits (1511), Expect = 4.4e-166
Identity = 388/823 (47.14%), Postives = 510/823 (61.97%), Query Frame = 0

Query: 33  AGNTVFKSGPLFLSSKGIGWTSWKKRWFILTRTSLVFFRSDPNAVPQKGGEANLTLGGID 92
           A NTV+KSGPLF+SSKG+GWTSWKKRWFILTRTSLVFF++DP+A+PQKGGE NLTLGGID
Sbjct: 15  ASNTVYKSGPLFISSKGLGWTSWKKRWFILTRTSLVFFKNDPSALPQKGGEVNLTLGGID 74

Query: 93  LNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEWKAALENALAQAPSSA--- 152
           LN+SGSVVV+ +KKLLTVLFPDGR+GRAFTLKAETL+DLYEWKAALE ALAQAP++A   
Sbjct: 75  LNSSGSVVVREDKKLLTVLFPDGRDGRAFTLKAETLDDLYEWKAALEQALAQAPNAALVI 134

Query: 153 NANGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDVDGTPSFLEKALRFIE 212
             NGI + +       S  + +D +P   +  V+GRPILLALE++DG+PSFLEKAL+F+E
Sbjct: 135 GQNGIFRTEANNTIEASFNSWRDQRPL--KSSVVGRPILLALEEIDGSPSFLEKALQFLE 194

Query: 213 THGVKVEGILRQAADVDDVEHRLRGYEQGKIEFSPEEDAHVVGDCVKYVIRELPSSPVPA 272
           T+G KVEGILRQ+ADV++VE R++ YEQGK EFSPEED HVVGDCVK+V+R+LPSSPVPA
Sbjct: 195 TYGTKVEGILRQSADVEEVERRVQEYEQGKTEFSPEEDPHVVGDCVKHVLRQLPSSPVPA 254

Query: 273 SCCNALLEASKTERG-GRVNAMRSAIYETFPEPNRRLLQRILMMMQAVASHKAENRMSSS 332
           SCC ALLEA K ++   RVN++RSAI ETFPEPNRRLL R+L MM  + SH +ENRM+SS
Sbjct: 255 SCCTALLEAYKIDQNEARVNSLRSAIIETFPEPNRRLLLRMLKMMHTITSHSSENRMTSS 314

Query: 333 AVAACMAPLLLRPLLAGECEIETDFDVGGDGSIQLLRAAAAANHAQAIVITLLEEYGKIF 392
           AVAACM+PLLLRPLLAGEC++E  FD  GD S QLL AA AAN+AQAIV  LLE+YG + 
Sbjct: 315 AVAACMSPLLLRPLLAGECDLE-GFDTLGDNSAQLLAAANAANNAQAIVTALLEDYGNMI 374

Query: 393 GEGSMSPILYSDSEESGSESEEASDDD----MSYDDEEQDDATGSDVATDDELESAGTCS 452
            +  +     S     G    E S D+    + + D    D    +   D+++  +   S
Sbjct: 375 NDEGLGRCSTSTDSHIGDSGPENSSDEEEIVVKHPDLHTLDIEEGETDDDNDVLLSRKPS 434

Query: 453 GSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVN-GMKPKSSKERIHDKIDRGRGNSPIKDE 512
            S      DLYD KG  V  + +++    D++  ++      R+   I+      PIKD 
Sbjct: 435 ESSDYAGSDLYDYKGFGVEDSDAESPR--DIHCSVESTDFSARVKKHIE-----EPIKDI 494

Query: 513 KSCEV----ECASESFEGSPT----------------------TSNKSSNTTKRLTIWGR 572
           +   V     C     E  P+                      T+  S+  +KR + WGR
Sbjct: 495 EVSSVSPTENCYQSGREAIPSVTPSTPLTALRYTTSAEKPASKTTGSSTVNSKRSSSWGR 554

Query: 573 TPAKKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQER 632
              KK  +  S D    +E+ IQRLE  K EL+ +I + A+ NA LQ+SLE RK+ L ER
Sbjct: 555 GNGKKTPAKGSFDSSGNDELLIQRLEHMKDELRQRIAKEAKGNAALQASLERRKQALHER 614

Query: 633 RLTLEQEVARLREQLQREKD----------------PSANISEKTKADLGESNRGEKDTA 692
           RL LEQ+V RL+EQLQ E+D                 S     KT+A+L E    E D A
Sbjct: 615 RLALEQDVGRLQEQLQAERDLRSALEVGLSISCGQFSSQAADSKTRAELEEIALAEADVA 674

Query: 693 NINNTMDEFVGLLNRIRDQK-SNLPDLRNVSQQMQNHQTKLKDKRKDAGS-LAPSHPEHS 752
            +   + E    L++ R    S+LPD ++  Q + NH T+LK  ++D  S LA  + E +
Sbjct: 675 RLKQKVAELHHQLSQQRQHHLSSLPDAQSHHQFLHNHNTQLKSFQQDFDSILAFVNHERN 734

Query: 753 RNKDVLSGHAEISKHSPLHQHLDPHNPS---AKIGVLATNFATGLTNPKRTGARMELGPN 796
           +  D  S  A+       ++ + P +PS   A +G+    ++            M+ G +
Sbjct: 735 QRTDETSLRADWRNGRGNNRQV-PGSPSLNAASLGIPMEEYS----------PVMDYGRH 794

BLAST of CmaCh12G001900 vs. ExPASy Swiss-Prot
Match: Q91YM2 (Rho GTPase-activating protein 35 OS=Mus musculus OX=10090 GN=Arhgap35 PE=1 SV=3)

HSP 1 Score: 65.5 bits (158), Expect = 3.4e-09
Identity = 43/161 (26.71%), Postives = 80/161 (49.69%), Query Frame = 0

Query: 184  GRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKIEFS 243
            G P+   +      P F+E+ + +IE  G+  EGI R + +  ++E   R ++Q      
Sbjct: 1248 GVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDL 1307

Query: 244  PEED--AHVVGDCVKYVIRELPSSPVPASCCNALLEASK-TERGGRVNAMRSAIYETFPE 303
             E+D   + V   +K    ELP   VP S    L+EA K  +R  +++A++  + + FP+
Sbjct: 1308 AEKDFTVNTVAGAMKSFFSELPDPLVPYSMQIDLVEAHKINDREQKLHALKE-VLKKFPK 1367

Query: 304  PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRP 342
             N  + + ++  +  V+ +   N M+S  ++ C  P L+RP
Sbjct: 1368 ENHEVFKYVISHLNKVSHNNKVNLMTSENLSICFWPTLMRP 1407

BLAST of CmaCh12G001900 vs. ExPASy Swiss-Prot
Match: P81128 (Rho GTPase-activating protein 35 OS=Rattus norvegicus OX=10116 GN=Arhgap35 PE=1 SV=3)

HSP 1 Score: 65.5 bits (158), Expect = 3.4e-09
Identity = 43/161 (26.71%), Postives = 80/161 (49.69%), Query Frame = 0

Query: 184  GRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKIEFS 243
            G P+   +      P F+E+ + +IE  G+  EGI R + +  ++E   R ++Q      
Sbjct: 1248 GVPLTTVVTPEKPIPIFIERCIEYIEATGLSTEGIYRVSGNKSEMESLQRQFDQDHNLDL 1307

Query: 244  PEED--AHVVGDCVKYVIRELPSSPVPASCCNALLEASK-TERGGRVNAMRSAIYETFPE 303
             E+D   + V   +K    ELP   VP S    L+EA K  +R  +++A++  + + FP+
Sbjct: 1308 AEKDFTVNTVAGAMKSFFSELPDPLVPYSMQIDLVEAHKINDREQKLHALKE-VLKKFPK 1367

Query: 304  PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRP 342
             N  + + ++  +  V+ +   N M+S  ++ C  P L+RP
Sbjct: 1368 ENHEVFKYVISHLNRVSHNNKVNLMTSENLSICFWPTLMRP 1407

BLAST of CmaCh12G001900 vs. ExPASy TrEMBL
Match: A0A6J1KRY8 (rho GTPase-activating protein REN1 isoform X1 OS=Cucurbita maxima OX=3661 GN=LOC111495950 PE=4 SV=1)

HSP 1 Score: 1612.0 bits (4173), Expect = 0.0e+00
Identity = 847/847 (100.00%), Postives = 847/847 (100.00%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60
           MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF
Sbjct: 1   MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60

Query: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120
           ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA
Sbjct: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120

Query: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180
           FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP
Sbjct: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180

Query: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI 240
           RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI
Sbjct: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI 240

Query: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300
           EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE
Sbjct: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300

Query: 301 PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS 360
           PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS
Sbjct: 301 PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS 360

Query: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420
           IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD
Sbjct: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420

Query: 421 EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP 480
           EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP
Sbjct: 421 EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP 480

Query: 481 KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540
           KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA
Sbjct: 481 KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540

Query: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT 600
           KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT
Sbjct: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT 600

Query: 601 LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660
           LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ
Sbjct: 601 LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660

Query: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH 720
           KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH
Sbjct: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH 720

Query: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780
           LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA
Sbjct: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780

Query: 781 NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840
           NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ
Sbjct: 781 NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840

Query: 841 PQKSDAR 848
           PQKSDAR
Sbjct: 841 PQKSDAR 847

BLAST of CmaCh12G001900 vs. ExPASy TrEMBL
Match: A0A6J1GH90 (rho GTPase-activating protein REN1 isoform X1 OS=Cucurbita moschata OX=3662 GN=LOC111454154 PE=4 SV=1)

HSP 1 Score: 1572.8 bits (4071), Expect = 0.0e+00
Identity = 828/847 (97.76%), Postives = 834/847 (98.47%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60
           MTNRNAEPFQGESD PPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF
Sbjct: 1   MTNRNAEPFQGESDDPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60

Query: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120
           ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA
Sbjct: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120

Query: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180
           FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP
Sbjct: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180

Query: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI 240
           RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGK 
Sbjct: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKN 240

Query: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300
           EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE
Sbjct: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300

Query: 301 PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS 360
           PNRRLLQRILMMMQ VASHKAENRMSSSAVAACMAPLLLRPLLAG+CEIETDFDVGGDGS
Sbjct: 301 PNRRLLQRILMMMQTVASHKAENRMSSSAVAACMAPLLLRPLLAGDCEIETDFDVGGDGS 360

Query: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420
           IQLLRAAAAANHAQAIVITLLEEY KIFGEGSMSPILYSDSEESGSESEEASD DMSYDD
Sbjct: 361 IQLLRAAAAANHAQAIVITLLEEYDKIFGEGSMSPILYSDSEESGSESEEASDGDMSYDD 420

Query: 421 EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP 480
           EEQDDATGSDVATDDELES GTC+ SVGSGEHDLYDDKGS+VLSTSSKNSDMCDVNGMKP
Sbjct: 421 EEQDDATGSDVATDDELESTGTCTESVGSGEHDLYDDKGSEVLSTSSKNSDMCDVNGMKP 480

Query: 481 KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540
           KSSKERIHDK DRGRG+SPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA
Sbjct: 481 KSSKERIHDKTDRGRGSSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540

Query: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT 600
           KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENA+LQSSLENRKKILQERRLT
Sbjct: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAILQSSLENRKKILQERRLT 600

Query: 601 LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660
           LEQEVARLREQLQREKDPSAN+SEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ
Sbjct: 601 LEQEVARLREQLQREKDPSANVSEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660

Query: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH 720
           KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSL P  PEHSRNKDVLSGHAEISKHSPLHQH
Sbjct: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLVP--PEHSRNKDVLSGHAEISKHSPLHQH 720

Query: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780
           LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA
Sbjct: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780

Query: 781 NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840
           NELQNMDRGRVSGQS DGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQD DKRAGSQGSQ
Sbjct: 781 NELQNMDRGRVSGQSTDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDFDKRAGSQGSQ 840

Query: 841 PQKSDAR 848
           PQKSDAR
Sbjct: 841 PQKSDAR 845

BLAST of CmaCh12G001900 vs. ExPASy TrEMBL
Match: A0A6J1GI68 (rho GTPase-activating protein REN1 isoform X2 OS=Cucurbita moschata OX=3662 GN=LOC111454154 PE=4 SV=1)

HSP 1 Score: 1421.8 bits (3679), Expect = 0.0e+00
Identity = 755/773 (97.67%), Postives = 761/773 (98.45%), Query Frame = 0

Query: 75  NAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEW 134
           NAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEW
Sbjct: 37  NAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEW 96

Query: 135 KAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDV 194
           KAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDV
Sbjct: 97  KAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDV 156

Query: 195 DGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKIEFSPEEDAHVVGDC 254
           DGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGK EFSPEEDAHVVGDC
Sbjct: 157 DGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKNEFSPEEDAHVVGDC 216

Query: 255 VKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPEPNRRLLQRILMMMQ 314
           VKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPEPNRRLLQRILMMMQ
Sbjct: 217 VKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPEPNRRLLQRILMMMQ 276

Query: 315 AVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGSIQLLRAAAAANHAQ 374
            VASHKAENRMSSSAVAACMAPLLLRPLLAG+CEIETDFDVGGDGSIQLLRAAAAANHAQ
Sbjct: 277 TVASHKAENRMSSSAVAACMAPLLLRPLLAGDCEIETDFDVGGDGSIQLLRAAAAANHAQ 336

Query: 375 AIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDDEEQDDATGSDVATD 434
           AIVITLLEEY KIFGEGSMSPILYSDSEESGSESEEASD DMSYDDEEQDDATGSDVATD
Sbjct: 337 AIVITLLEEYDKIFGEGSMSPILYSDSEESGSESEEASDGDMSYDDEEQDDATGSDVATD 396

Query: 435 DELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKPKSSKERIHDKIDRG 494
           DELES GTC+ SVGSGEHDLYDDKGS+VLSTSSKNSDMCDVNGMKPKSSKERIHDK DRG
Sbjct: 397 DELESTGTCTESVGSGEHDLYDDKGSEVLSTSSKNSDMCDVNGMKPKSSKERIHDKTDRG 456

Query: 495 RGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPAKKKLSLESIDYDFG 554
           RG+SPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPAKKKLSLESIDYDFG
Sbjct: 457 RGSSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPAKKKLSLESIDYDFG 516

Query: 555 EEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLTLEQEVARLREQLQR 614
           EEVEIQRLEATKCELQNKIVEAARENA+LQSSLENRKKILQERRLTLEQEVARLREQLQR
Sbjct: 517 EEVEIQRLEATKCELQNKIVEAARENAILQSSLENRKKILQERRLTLEQEVARLREQLQR 576

Query: 615 EKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQKSNLPDLRNVSQQM 674
           EKDPSAN+SEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQKSNLPDLRNVSQQM
Sbjct: 577 EKDPSANVSEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQKSNLPDLRNVSQQM 636

Query: 675 QNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQHLDPHNPSAKIGVLA 734
           QNHQTKLKDKRKDAGSL P  PEHSRNKDVLSGHAEISKHSPLHQHLDPHNPSAKIGVLA
Sbjct: 637 QNHQTKLKDKRKDAGSLVP--PEHSRNKDVLSGHAEISKHSPLHQHLDPHNPSAKIGVLA 696

Query: 735 TNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIANELQNMDRGRVSGQ 794
           TNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIANELQNMDRGRVSGQ
Sbjct: 697 TNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIANELQNMDRGRVSGQ 756

Query: 795 SIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQPQKSDAR 848
           S DGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQD DKRAGSQGSQPQKSDAR
Sbjct: 757 STDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDFDKRAGSQGSQPQKSDAR 807

BLAST of CmaCh12G001900 vs. ExPASy TrEMBL
Match: A0A0A0LP02 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G372110 PE=4 SV=1)

HSP 1 Score: 1206.0 bits (3119), Expect = 0.0e+00
Identity = 693/950 (72.95%), Postives = 753/950 (79.26%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60
           MTNRN EP Q E  +PPPPPPPPP DP+ GS AGNTVFKSGPLFLSSKGIGWTSWKKRWF
Sbjct: 1   MTNRNVEPSQLECGAPPPPPPPPPHDPSAGSRAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60

Query: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120
           ILTRTSLVFFRSDPNAVPQKGGE NLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA
Sbjct: 61  ILTRTSLVFFRSDPNAVPQKGGEVNLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120

Query: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180
           FTLKAETLEDLYEWKAALENALAQAPS+A+ NGILKNDK+E N+GSSETLKD QPQP RP
Sbjct: 121 FTLKAETLEDLYEWKAALENALAQAPSTAHGNGILKNDKIESNDGSSETLKDNQPQPVRP 180

Query: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI 240
           RVLGRPILLALEDVDGTPSFLEKALRFIE HGVKVEGILRQAADVDDVE R+R YEQGK 
Sbjct: 181 RVLGRPILLALEDVDGTPSFLEKALRFIEGHGVKVEGILRQAADVDDVERRVRDYEQGKN 240

Query: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300
           EFS EEDAHVV DCVKYVIRELPSSPVPASCCNALLEA KT+RG RVNAMRSAI ETFPE
Sbjct: 241 EFSSEEDAHVVADCVKYVIRELPSSPVPASCCNALLEACKTDRGIRVNAMRSAICETFPE 300

Query: 301 PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS 360
           PNRRLLQRILMMMQ VASHKAENRMSSSAVAACMAPLLLRPLLAG+CEIETDFDVGGDGS
Sbjct: 301 PNRRLLQRILMMMQTVASHKAENRMSSSAVAACMAPLLLRPLLAGDCEIETDFDVGGDGS 360

Query: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420
           IQLLRAAAAANHAQAIVITLLEEYGKIFGE SMSPI+YSDS+ESGSESEEA+DD+MSYDD
Sbjct: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGECSMSPIMYSDSDESGSESEEATDDEMSYDD 420

Query: 421 EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP 480
           E+QDD TGSD  T DELES+GTCSGSV S +H+ YDDKGS+V S+SSKNSD C VNG KP
Sbjct: 421 EDQDDVTGSDAETGDELESSGTCSGSVASEDHE-YDDKGSEVSSSSSKNSDACQVNGTKP 480

Query: 481 KSS----------------KERIHDKIDRGRGNSPIKDEKSCEVECA------------- 540
           K S                KE I +K D G+ NSPIKDEK   VECA             
Sbjct: 481 KLSSSSPKTSLPQRGEVQNKESIQNKDDPGKDNSPIKDEKPSAVECASNEANMTNKLDVC 540

Query: 541 -SESFEGSPTTSNKSSN-TTKRLTIWGRTP----------------------AKKKLSLE 600
            S SFEGSPTTSNK+S+ +++RLT+WGRTP                      AKK LS+E
Sbjct: 541 PSSSFEGSPTTSNKTSHVSSRRLTVWGRTPHILFFHVPAGSLTSFYFLIYVQAKKNLSME 600

Query: 601 SIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLTLEQEVAR 660
           S+DYDFGEEVEIQRLEATK ELQNKI+E A+ENA LQSSLENRKK LQERRLTLEQEVAR
Sbjct: 601 SMDYDFGEEVEIQRLEATKGELQNKILEEAKENAALQSSLENRKKALQERRLTLEQEVAR 660

Query: 661 LREQLQREKD----------------PS-ANISEKTKADLGESNRGEKDTANINNTMDEF 720
           L+EQLQ+E+D                P+ ANISEKTKADL E ++ EKD AN+NN ++ +
Sbjct: 661 LKEQLQKERDLRMALETGLKISQGPLPNLANISEKTKADLEEIDQAEKDIANLNNMVNAY 720

Query: 721 VGLLNRIRDQKSNL-PDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHA 780
            G L+ +RDQK+NL PD RNVSQQ QNHQTK KDK+KDAG+  PSH EHSRNKDVLSG A
Sbjct: 721 GGQLDMLRDQKNNLSPDSRNVSQQEQNHQTKSKDKKKDAGAFGPSHSEHSRNKDVLSGQA 780

Query: 781 E------------ISKHSPLHQHLDPHNPSAKIGVLATNFATG------LTNPKRTGARM 840
           E             SKH PLHQH DP N + +   L TN +         T PKRTGAR+
Sbjct: 781 ENDNEKKMESSSSASKHPPLHQHPDPRNATVRSLGLPTNSSASETLPARPTAPKRTGARI 840

Query: 841 ELGPNHNSSALTKLTTRLNFLKERRSQIANELQNMDRGRVSGQSIDGSEKGRGVEAQRSV 847
           E GPNH SSALTKLTTRLNFLKERRSQIANELQNMDRGRVS Q  +  +K RG+EAQRS+
Sbjct: 841 E-GPNHTSSALTKLTTRLNFLKERRSQIANELQNMDRGRVSSQPFENLDKSRGLEAQRSL 900

BLAST of CmaCh12G001900 vs. ExPASy TrEMBL
Match: A0A1S4DW13 (rho GTPase-activating protein REN1 OS=Cucumis melo OX=3656 GN=LOC103488140 PE=4 SV=1)

HSP 1 Score: 1204.9 bits (3116), Expect = 0.0e+00
Identity = 689/927 (74.33%), Postives = 751/927 (81.01%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPP-PPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRW 60
           M+NRN EP Q ES +PPP PPPPPP DP+ GS AGNTVFKSGPLFLSSKGIGWTSWKKRW
Sbjct: 1   MSNRNVEPSQVESGAPPPAPPPPPPHDPSAGSRAGNTVFKSGPLFLSSKGIGWTSWKKRW 60

Query: 61  FILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGR 120
           FILTRTSLVFFRSDPNAVPQKGGE NLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGR
Sbjct: 61  FILTRTSLVFFRSDPNAVPQKGGEVNLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGR 120

Query: 121 AFTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGR 180
           AFTLKAETLEDLYEWK ALENALAQAPS+A+ANGILKNDK+E N+GSSETLKD QPQP R
Sbjct: 121 AFTLKAETLEDLYEWKVALENALAQAPSTAHANGILKNDKIESNDGSSETLKDNQPQPVR 180

Query: 181 PRVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGK 240
           PRVLGRPILLALEDVDGTPSFLEKALRFIE HGVKVEGILRQAADVDDVE R+R YEQGK
Sbjct: 181 PRVLGRPILLALEDVDGTPSFLEKALRFIEGHGVKVEGILRQAADVDDVERRVRDYEQGK 240

Query: 241 IEFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFP 300
            EFS EEDAHVV DCVKYVIRELPSSPVPASCCNALLEA KT+RG RVNAMRSAIYETFP
Sbjct: 241 NEFSSEEDAHVVADCVKYVIRELPSSPVPASCCNALLEACKTDRGIRVNAMRSAIYETFP 300

Query: 301 EPNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDG 360
           EPNRRLLQRILMMMQ VASHKAENRMSSSAVAACMAPLLLRPLLAG+CEIETDFDVGGDG
Sbjct: 301 EPNRRLLQRILMMMQTVASHKAENRMSSSAVAACMAPLLLRPLLAGDCEIETDFDVGGDG 360

Query: 361 SIQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYD 420
           SIQLLRAAAAANHAQAIVITLLEEYGKIFGE SMSPI+YSDSEESGSESEEA+DD+MSYD
Sbjct: 361 SIQLLRAAAAANHAQAIVITLLEEYGKIFGECSMSPIMYSDSEESGSESEEATDDEMSYD 420

Query: 421 DEEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMK 480
           DEEQDD TGSD  T DELES+GTCSGSV S + + YDDKGS+V S+SSKNSD C VNG K
Sbjct: 421 DEEQDDVTGSDAETGDELESSGTCSGSVDSEDRE-YDDKGSEVSSSSSKNSDACKVNGTK 480

Query: 481 PKSS----------------KERIHDKIDRGRGNSPIKDEKSCEVECASE---------- 540
           PK S                KE I +K D GR NSPIKDE+  EVECAS+          
Sbjct: 481 PKFSSSSPKTSLPQRGEVQNKESIQNKDDPGRDNSPIKDERP-EVECASDEANMTNKLDL 540

Query: 541 ----SFEGSPTTSNKSSNTTKRLTIWGRTPAKKKLSLESIDYDFGEEVEIQRLEATKCEL 600
               S EGSPTTSNKSS+ ++RLT+WGRTPAKK LS+ES+DYDFGEE EIQRLEATK EL
Sbjct: 541 CPSSSVEGSPTTSNKSSHASRRLTVWGRTPAKKNLSMESVDYDFGEEFEIQRLEATKGEL 600

Query: 601 QNKIVEAARENAVLQSSLENRKKILQERRLTLEQEVARLREQLQREKD------------ 660
           QNKI+E A+ENA LQS LENRKK L ERRLTLEQEVARL+EQLQ+E+D            
Sbjct: 601 QNKILEEAKENAALQSCLENRKKALLERRLTLEQEVARLKEQLQKERDLRMALEAGLKIS 660

Query: 661 ----PS-ANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQKSNL-PDLRNVS 720
               P+ ANI+EKTKADL E ++ EKD AN+NN +++F G L+ +RDQK+NL  D RNVS
Sbjct: 661 QGPLPNLANINEKTKADLEEIDQAEKDIANLNNMVNDFGGQLDMLRDQKNNLSSDSRNVS 720

Query: 721 QQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAE------------ISKHSPLHQ 780
           +Q QN+QTK KDK+KDAG+L PSH EHSRNKDVLSG AE             SK+ PLHQ
Sbjct: 721 KQEQNYQTKSKDKKKDAGALGPSHSEHSRNKDVLSGQAENDNEKKMEPSSSASKYPPLHQ 780

Query: 781 HLDPHNPSAKIGVLATNFATG------LTNPKRTGARMELGPNHNSSALTKLTTRLNFLK 840
           H DP N + +   L TN +T        T PKRTGAR+E GPNH SSALTKLTTRLNFLK
Sbjct: 781 HPDPRNAAVRSVGLPTNSSTSETLPSRPTAPKRTGARIE-GPNHTSSALTKLTTRLNFLK 840

Query: 841 ERRSQIANELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSAD----------- 847
           ERRSQIANELQNMDRGR   Q  +  +K RG E+QRS+QNSD+T+GS+D           
Sbjct: 841 ERRSQIANELQNMDRGR--SQPFENLDKSRGPESQRSLQNSDETQGSSDVQPMRNPETSR 900

BLAST of CmaCh12G001900 vs. NCBI nr
Match: XP_023001913.1 (rho GTPase-activating protein REN1 isoform X1 [Cucurbita maxima])

HSP 1 Score: 1612.0 bits (4173), Expect = 0.0e+00
Identity = 847/847 (100.00%), Postives = 847/847 (100.00%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60
           MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF
Sbjct: 1   MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60

Query: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120
           ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA
Sbjct: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120

Query: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180
           FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP
Sbjct: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180

Query: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI 240
           RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI
Sbjct: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI 240

Query: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300
           EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE
Sbjct: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300

Query: 301 PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS 360
           PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS
Sbjct: 301 PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS 360

Query: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420
           IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD
Sbjct: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420

Query: 421 EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP 480
           EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP
Sbjct: 421 EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP 480

Query: 481 KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540
           KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA
Sbjct: 481 KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540

Query: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT 600
           KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT
Sbjct: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT 600

Query: 601 LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660
           LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ
Sbjct: 601 LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660

Query: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH 720
           KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH
Sbjct: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH 720

Query: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780
           LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA
Sbjct: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780

Query: 781 NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840
           NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ
Sbjct: 781 NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840

Query: 841 PQKSDAR 848
           PQKSDAR
Sbjct: 841 PQKSDAR 847

BLAST of CmaCh12G001900 vs. NCBI nr
Match: XP_023537172.1 (rho GTPase-activating protein REN1 isoform X1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1576.2 bits (4080), Expect = 0.0e+00
Identity = 830/847 (97.99%), Postives = 835/847 (98.58%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60
           MTNRNAEPFQGESD PPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF
Sbjct: 1   MTNRNAEPFQGESDDPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60

Query: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120
           ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA
Sbjct: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120

Query: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180
           FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP
Sbjct: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180

Query: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI 240
           RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGK 
Sbjct: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKN 240

Query: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300
           EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE
Sbjct: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300

Query: 301 PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS 360
           PNRRLLQRILMMMQ VASHKAENRMSSSAVAACMAPLLLRPLLAG+CEIETDFDVGGDGS
Sbjct: 301 PNRRLLQRILMMMQTVASHKAENRMSSSAVAACMAPLLLRPLLAGDCEIETDFDVGGDGS 360

Query: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420
           IQLLRAAAAANHAQAIVITLLEEY KIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD
Sbjct: 361 IQLLRAAAAANHAQAIVITLLEEYDKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420

Query: 421 EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP 480
           EEQDDATGSDVATDDELES GTCSGSV SGEHDLYDDKGS+VLSTSSKNSDMCDVNGMKP
Sbjct: 421 EEQDDATGSDVATDDELESTGTCSGSVVSGEHDLYDDKGSEVLSTSSKNSDMCDVNGMKP 480

Query: 481 KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540
           KSSKERIHDK DRGRG+SPIKDEKSCEVECASESFEGSPTTSNK SNTTKRLTIWGRTPA
Sbjct: 481 KSSKERIHDKTDRGRGSSPIKDEKSCEVECASESFEGSPTTSNKGSNTTKRLTIWGRTPA 540

Query: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT 600
           KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT
Sbjct: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT 600

Query: 601 LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660
           LEQEVARLREQLQREKDPSAN+SEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ
Sbjct: 601 LEQEVARLREQLQREKDPSANVSEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660

Query: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH 720
           KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSL P  PEHSRNKDVLSGHAEISKHSPLHQH
Sbjct: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLVP--PEHSRNKDVLSGHAEISKHSPLHQH 720

Query: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780
           LDPHNPSAKIGVLATNFATGLTNPKR GARMELGPNHNSSALTKLTTRLNFLKERRSQIA
Sbjct: 721 LDPHNPSAKIGVLATNFATGLTNPKRAGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780

Query: 781 NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840
           NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQG+Q
Sbjct: 781 NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGNQ 840

Query: 841 PQKSDAR 848
           PQKSDAR
Sbjct: 841 PQKSDAR 845

BLAST of CmaCh12G001900 vs. NCBI nr
Match: KAG6585329.1 (Rho GTPase-activating protein REN1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1574.7 bits (4076), Expect = 0.0e+00
Identity = 829/847 (97.87%), Postives = 835/847 (98.58%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60
           MTNRNAEPFQGESD PPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF
Sbjct: 1   MTNRNAEPFQGESDDPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60

Query: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120
           ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA
Sbjct: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120

Query: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180
           FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP
Sbjct: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180

Query: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI 240
           RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLR YEQGK 
Sbjct: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRVYEQGKN 240

Query: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300
           EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE
Sbjct: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300

Query: 301 PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS 360
           PNRRLLQRILMMMQ VASHKAENRMSSSAVAACMAPLLLRPLLAG+CEIETDFDVGGDGS
Sbjct: 301 PNRRLLQRILMMMQTVASHKAENRMSSSAVAACMAPLLLRPLLAGDCEIETDFDVGGDGS 360

Query: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420
           IQLLRAAAAANHAQAIVITLLEEY KIFGEGSMSPILYSDSEESGSESEEASD DMSYDD
Sbjct: 361 IQLLRAAAAANHAQAIVITLLEEYDKIFGEGSMSPILYSDSEESGSESEEASDGDMSYDD 420

Query: 421 EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP 480
           EEQDDATGSDVATDDELES GTC+GSVGSGEHDLYDDKGS+VLSTSSKNSDMCDVNGMKP
Sbjct: 421 EEQDDATGSDVATDDELESTGTCTGSVGSGEHDLYDDKGSEVLSTSSKNSDMCDVNGMKP 480

Query: 481 KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540
           KSSKERIHDK DRGRG+SPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA
Sbjct: 481 KSSKERIHDKTDRGRGSSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540

Query: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT 600
           KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENA+LQSSLENRKKILQERRLT
Sbjct: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAILQSSLENRKKILQERRLT 600

Query: 601 LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660
           LEQEVARLREQLQREKDPSAN+SEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ
Sbjct: 601 LEQEVARLREQLQREKDPSANVSEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660

Query: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH 720
           KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSL P  PEHSRNKDVLSGHAEISKHSPLHQH
Sbjct: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLVP--PEHSRNKDVLSGHAEISKHSPLHQH 720

Query: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780
           LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA
Sbjct: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780

Query: 781 NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840
           NELQNMDRGRVSGQS DGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ
Sbjct: 781 NELQNMDRGRVSGQSTDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840

Query: 841 PQKSDAR 848
           PQKSDAR
Sbjct: 841 PQKSDAR 845

BLAST of CmaCh12G001900 vs. NCBI nr
Match: XP_022951263.1 (rho GTPase-activating protein REN1 isoform X1 [Cucurbita moschata])

HSP 1 Score: 1572.8 bits (4071), Expect = 0.0e+00
Identity = 828/847 (97.76%), Postives = 834/847 (98.47%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60
           MTNRNAEPFQGESD PPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF
Sbjct: 1   MTNRNAEPFQGESDDPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60

Query: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120
           ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA
Sbjct: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120

Query: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180
           FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP
Sbjct: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180

Query: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI 240
           RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGK 
Sbjct: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKN 240

Query: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300
           EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE
Sbjct: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300

Query: 301 PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS 360
           PNRRLLQRILMMMQ VASHKAENRMSSSAVAACMAPLLLRPLLAG+CEIETDFDVGGDGS
Sbjct: 301 PNRRLLQRILMMMQTVASHKAENRMSSSAVAACMAPLLLRPLLAGDCEIETDFDVGGDGS 360

Query: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420
           IQLLRAAAAANHAQAIVITLLEEY KIFGEGSMSPILYSDSEESGSESEEASD DMSYDD
Sbjct: 361 IQLLRAAAAANHAQAIVITLLEEYDKIFGEGSMSPILYSDSEESGSESEEASDGDMSYDD 420

Query: 421 EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP 480
           EEQDDATGSDVATDDELES GTC+ SVGSGEHDLYDDKGS+VLSTSSKNSDMCDVNGMKP
Sbjct: 421 EEQDDATGSDVATDDELESTGTCTESVGSGEHDLYDDKGSEVLSTSSKNSDMCDVNGMKP 480

Query: 481 KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540
           KSSKERIHDK DRGRG+SPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA
Sbjct: 481 KSSKERIHDKTDRGRGSSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540

Query: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT 600
           KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENA+LQSSLENRKKILQERRLT
Sbjct: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAILQSSLENRKKILQERRLT 600

Query: 601 LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660
           LEQEVARLREQLQREKDPSAN+SEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ
Sbjct: 601 LEQEVARLREQLQREKDPSANVSEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660

Query: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH 720
           KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSL P  PEHSRNKDVLSGHAEISKHSPLHQH
Sbjct: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLVP--PEHSRNKDVLSGHAEISKHSPLHQH 720

Query: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780
           LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA
Sbjct: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780

Query: 781 NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840
           NELQNMDRGRVSGQS DGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQD DKRAGSQGSQ
Sbjct: 781 NELQNMDRGRVSGQSTDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDFDKRAGSQGSQ 840

Query: 841 PQKSDAR 848
           PQKSDAR
Sbjct: 841 PQKSDAR 845

BLAST of CmaCh12G001900 vs. NCBI nr
Match: KAG7020241.1 (Rho GTPase-activating protein REN1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1564.7 bits (4050), Expect = 0.0e+00
Identity = 826/847 (97.52%), Postives = 832/847 (98.23%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60
           MTNRNAEPFQGESD PPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF
Sbjct: 1   MTNRNAEPFQGESDDPPPPPPPPPQDPADGSCAGNTVFKSGPLFLSSKGIGWTSWKKRWF 60

Query: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120
           ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA
Sbjct: 61  ILTRTSLVFFRSDPNAVPQKGGEANLTLGGIDLNNSGSVVVKSEKKLLTVLFPDGREGRA 120

Query: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180
           FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP
Sbjct: 121 FTLKAETLEDLYEWKAALENALAQAPSSANANGILKNDKMEPNNGSSETLKDGQPQPGRP 180

Query: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRGYEQGKI 240
           RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLR YEQGK 
Sbjct: 181 RVLGRPILLALEDVDGTPSFLEKALRFIETHGVKVEGILRQAADVDDVEHRLRVYEQGKN 240

Query: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300
           EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE
Sbjct: 241 EFSPEEDAHVVGDCVKYVIRELPSSPVPASCCNALLEASKTERGGRVNAMRSAIYETFPE 300

Query: 301 PNRRLLQRILMMMQAVASHKAENRMSSSAVAACMAPLLLRPLLAGECEIETDFDVGGDGS 360
           PNRRLLQRILMMMQ VASHKAENRMSSSAVAACMAPLLLRPLLAG+CEIETDFDVGGDGS
Sbjct: 301 PNRRLLQRILMMMQTVASHKAENRMSSSAVAACMAPLLLRPLLAGDCEIETDFDVGGDGS 360

Query: 361 IQLLRAAAAANHAQAIVITLLEEYGKIFGEGSMSPILYSDSEESGSESEEASDDDMSYDD 420
           IQLLRAAAAANHAQAIVITLLEEY KIFGEGSMSPILYSDSEESGSESEEASD DMSYDD
Sbjct: 361 IQLLRAAAAANHAQAIVITLLEEYDKIFGEGSMSPILYSDSEESGSESEEASDGDMSYDD 420

Query: 421 EEQDDATGSDVATDDELESAGTCSGSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVNGMKP 480
           EEQDDATGSDVATDDELES GTC+GSVGSGEHDLYDDK   +LSTSSKNSDMCDVNGMKP
Sbjct: 421 EEQDDATGSDVATDDELESTGTCTGSVGSGEHDLYDDKA--LLSTSSKNSDMCDVNGMKP 480

Query: 481 KSSKERIHDKIDRGRGNSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540
           KSSKERIHDK DRGRG+SPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA
Sbjct: 481 KSSKERIHDKTDRGRGSSPIKDEKSCEVECASESFEGSPTTSNKSSNTTKRLTIWGRTPA 540

Query: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQERRLT 600
           KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENA+LQSSLENRKKILQERRLT
Sbjct: 541 KKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAILQSSLENRKKILQERRLT 600

Query: 601 LEQEVARLREQLQREKDPSANISEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660
           LEQEVARLREQLQREKDPSAN+SEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ
Sbjct: 601 LEQEVARLREQLQREKDPSANVSEKTKADLGESNRGEKDTANINNTMDEFVGLLNRIRDQ 660

Query: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAPSHPEHSRNKDVLSGHAEISKHSPLHQH 720
           KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSL P  PEHSRNKDVLSGHAEISKHSPLHQH
Sbjct: 661 KSNLPDLRNVSQQMQNHQTKLKDKRKDAGSLVP--PEHSRNKDVLSGHAEISKHSPLHQH 720

Query: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780
           LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA
Sbjct: 721 LDPHNPSAKIGVLATNFATGLTNPKRTGARMELGPNHNSSALTKLTTRLNFLKERRSQIA 780

Query: 781 NELQNMDRGRVSGQSIDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840
           NELQNMDRGRVSGQS DGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ
Sbjct: 781 NELQNMDRGRVSGQSTDGSEKGRGVEAQRSVQNSDDTKGSADNLQSIQDSDKRAGSQGSQ 840

Query: 841 PQKSDAR 848
           PQKSDAR
Sbjct: 841 PQKSDAR 843

BLAST of CmaCh12G001900 vs. TAIR 10
Match: AT4G24580.1 (Rho GTPase activation protein (RhoGAP) with PH domain )

HSP 1 Score: 713.8 bits (1841), Expect = 1.7e-205
Identity = 482/944 (51.06%), Postives = 595/944 (63.03%), Query Frame = 0

Query: 1   MTNRNAEPFQGESDSPPPPPPP-------PP------QDPADGSCA-------------- 60
           M N+NAE     S  PPP   P       PP      Q+P   +C+              
Sbjct: 1   MANKNAE----SSSQPPPHVQPNQQQQQQPPIANEQEQEPHGDTCSIPPAQSGNTDSRSR 60

Query: 61  -GNTVFKSGPLFLSSKGIGWTSWKKRWFILTRTSLVFFRSDPNAVPQKGGEANLTLGGID 120
            GNTVFKSGPL +SSKGIGWTSWKKRWFILTRTSLVFFRSDP+AV QKG E NLTLGGID
Sbjct: 61  GGNTVFKSGPLSISSKGIGWTSWKKRWFILTRTSLVFFRSDPSAVQQKGSEVNLTLGGID 120

Query: 121 LNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEWKAALENALAQAPSSANA- 180
           LNNSGSVVVK++KKLLTVLFPDGR+GRAFTLKA+T+EDL+EWKAALENAL QAPS+++  
Sbjct: 121 LNNSGSVVVKADKKLLTVLFPDGRDGRAFTLKADTMEDLHEWKAALENALTQAPSASHVM 180

Query: 181 --NGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDVDGTPSFLEKALRFIE 240
             NGI +ND  +P  G  E  KD    P +  VLGRP+LLALEDVDG PSFLEKALRF+E
Sbjct: 181 GQNGIFRNDHADPAVGVDEK-KD--ETPTKSTVLGRPVLLALEDVDGAPSFLEKALRFVE 240

Query: 241 THGVKVEGILRQAADVDDVEHRLRGYEQGKIEFSPEEDAHVVGDCVKYVIRELPSSPVPA 300
            HGV++EGILRQAADVDDVEHR+R YE+GK EFSPEEDAH++ DC+KY +RELPSSPVPA
Sbjct: 241 NHGVRIEGILRQAADVDDVEHRIREYEKGKNEFSPEEDAHIIADCLKYFLRELPSSPVPA 300

Query: 301 SCCNALLEASKTERGGRVNAMRSAIYETFPEPNRRLLQRILMMMQAVASHKAENRMSSSA 360
           SCCNALLEA +T+RG RVNAMR+AI E+FPEPNRRLLQRILMMMQ VAS+K  NRM+++A
Sbjct: 301 SCCNALLEACRTDRGNRVNAMRAAICESFPEPNRRLLQRILMMMQTVASNKTVNRMNTNA 360

Query: 361 VAACMAPLLLRPLLAGECEIETDFDVGGDGSIQLLRAAAAANHAQAIVITLLEEYGKIFG 420
           VAACMAPLLLRPLLAG+CEIE DFDVGGDGS+QLL+AAAAANHAQAIVITLLEEY  IFG
Sbjct: 361 VAACMAPLLLRPLLAGDCEIENDFDVGGDGSMQLLQAAAAANHAQAIVITLLEEYESIFG 420

Query: 421 -------------EGSMSPILYSDSEESGSESEEASDDDMSYDDEEQDDATGSDVATDDE 480
                        EGS+SP LYSDSEESGS +EE SDD+  YDD++ D + GS+  TD+E
Sbjct: 421 TLTSSIINGLCLQEGSLSPGLYSDSEESGSGTEEGSDDE-EYDDDD-DGSQGSEDYTDEE 480

Query: 481 --LESAGTCSGSVGSGEHDLY-------DDKGSDVLSTSSKNSDMCDVNGMKPKSSKERI 540
             LE+    S S  +   D Y       D K +D LST SK+             S+   
Sbjct: 481 EDLENESNGSYSESAASEDKYADSIDPDDHKINDNLSTESKSPKRSKEPKKLLSGSRRSS 540

Query: 541 HDKIDRGRGNSPI--KDEKSCEVEC---ASESFEGSPTTSNKSSNTTKRLTI-------- 600
             + D G+ +  I  K   + EV+     S S + + +TS+ +S+T K   +        
Sbjct: 541 LPRHDDGKKDEDIVVKGVNNTEVKAVVEVSTSEDKNSSTSDVASDTQKPSKLSDAPGGSK 600

Query: 601 --WGRTPAKKKLSLESIDYDF---GEEVEIQRLEATKCELQNKIVEAARENAVLQSSLEN 660
             WGRTP KK LS+ESID+      +  +I+RLE+TK ELQ++I E  + NAVLQ+SLE 
Sbjct: 601 RHWGRTPGKKNLSMESIDFSVEVDEDNADIERLESTKLELQSRITEEVKSNAVLQASLER 660

Query: 661 RKKILQERRLTLEQEVARLREQLQREKDPS-----------------ANISEKTKADLGE 720
           RKK L  RR  LEQ+V RL+EQLQ+E+D                     I E  K DL E
Sbjct: 661 RKKALYGRRQALEQDVGRLQEQLQQERDRKLALETGLNMSKGNQPIPETIDENLKKDLQE 720

Query: 721 SNRGEKDTANINNTMDEFVGLLNRIRDQKS-NLPDLRNVSQQMQNHQTKLKDKRKDAGSL 780
             + E D A + + +D+    L     + S +       S+++  H  K+K+K+KD  + 
Sbjct: 721 VAQAEADIAKLEHKVDDLENRLGHHDGKASGSTHSASKESRKLPEHNAKMKEKQKDTEAA 780

Query: 781 APSHPEHSRNKDVLSGHAEISKHSPLHQHLDPHNPSAKIGVLATNFATGLTNPKRTGARM 840
           +    E S +KD   G     ++    Q       S +     ++   GL+  KR+G + 
Sbjct: 781 STHISERSTSKD---GQGAARENETEKQQDSRSKSSQQETSRGSSKLVGLS--KRSGTKG 840

Query: 841 ELGPNHNSSALTKLTTRLNFLKERRSQIANELQNMDRGRVSGQSIDGSEKGRGVE----A 848
           E G    +SAL+KLT RLNFLKERRSQIANELQNMD+G+  GQ    S + R  E     
Sbjct: 841 E-GSTTTTSALSKLTMRLNFLKERRSQIANELQNMDKGKTLGQPSPTSGQNRVSEETEKG 900

BLAST of CmaCh12G001900 vs. TAIR 10
Match: AT5G19390.1 (Rho GTPase activation protein (RhoGAP) with PH domain )

HSP 1 Score: 593.2 bits (1528), Expect = 3.3e-169
Identity = 389/842 (46.20%), Postives = 511/842 (60.69%), Query Frame = 0

Query: 33  AGNTVFKSGPLFLSSKGIGWTSWKKRWFILTRTSLVFFRSDPNAVPQKGGEANLTLGGID 92
           A NTVFKSGPLF+SSKG+GWTSWKKRWFILTRTSLVFF++DP  +PQKGGE NLTLGGID
Sbjct: 15  ASNTVFKSGPLFISSKGLGWTSWKKRWFILTRTSLVFFKNDPGTLPQKGGEVNLTLGGID 74

Query: 93  LNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEWKAALENALAQAPSSA--- 152
           LNNSGSVVV+ +KKLLTVLFPDGR+GRAFTLKAET EDLYEWK ALE ALAQAP++A   
Sbjct: 75  LNNSGSVVVREDKKLLTVLFPDGRDGRAFTLKAETFEDLYEWKTALEQALAQAPNAALIM 134

Query: 153 NANGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDVDGTPSFLEKALRFIE 212
             NGI + +       ++E ++  + +P +  V+GRPILLALED+DG+PSFLEKAL+FIE
Sbjct: 135 GQNGIFRAE-------TNEAIEGREKRPLKSLVVGRPILLALEDIDGSPSFLEKALQFIE 194

Query: 213 THGVKVEGILRQAADVDDVEHRLRGYEQGKIEFSPEEDAHVVGDCVKYVIRELPSSPVPA 272
            +G K+EGILRQ+ADV++VE R++ YEQGK EF+ +ED HVVGDC+K+V+RELPSSPV A
Sbjct: 195 KYGTKIEGILRQSADVEEVERRVQEYEQGKTEFTFDEDPHVVGDCIKHVLRELPSSPVSA 254

Query: 273 SCCNALLEASKTE-RGGRVNAMRSAIYETFPEPNRRLLQRILMMMQAVASHKAENRMSSS 332
           SCC ALLEA + E +  R++++RSAI ETFPEPNRRLLQRIL MM  ++SH  ENRM+ +
Sbjct: 255 SCCTALLEAYRIESKEARISSLRSAIAETFPEPNRRLLQRILKMMHTISSHSHENRMNPN 314

Query: 333 AVAACMAPLLLRPLLAGECEIETDFDVGGDGSIQLLRAAAAANHAQAIVITLLEEYGKIF 392
           AVAACMAPLLLRPLLAGEC++E DFD G D S QLL AA AAN+AQAI+  LLE+YG IF
Sbjct: 315 AVAACMAPLLLRPLLAGECDLEDDFDGGEDNSAQLLAAANAANNAQAIITVLLEDYGSIF 374

Query: 393 GEGSMSPI-LYSDSEESGSESEEASDDDMSYDDEEQDDATGSDVATDDELESA--GTCSG 452
            E ++    + ++S    S  +++SDDD +  +   +     +  TDD+ + A  G  S 
Sbjct: 375 DEENIQRCSISTESHIGNSGPDDSSDDDNNMKNGYHNADNEVEPVTDDDNDRALSGKMSE 434

Query: 453 SVGSGEHDLYDDKG-----SDVLSTSSKNSDMCDVNGMKPKSSKERIHDKIDRGR----G 512
           S G    DLY+ KG     SD+ S    N   C+ N       +    +  D+      G
Sbjct: 435 SSGCTGSDLYEYKGFVADDSDIESPRDTNGPRCNSNIRTDHLMRNPFVNSTDQQAGEQIG 494

Query: 513 NSPIKDEKSCEVECASESFEGS------PTTSN-------------------KSSNTTKR 572
           + P K   +  +   SES++ S      PT  N                    SS   KR
Sbjct: 495 DDPTKYGVNSCLAHVSESYQQSGTGLNVPTHGNTLAAPGLESPSAKSVNKGTPSSVHAKR 554

Query: 573 LTIWGRTPAKKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRK 632
            T WGR  A+K  +  S D    +E+ IQRLE TK EL+ +I + AR NA+LQ+SLE RK
Sbjct: 555 ATFWGRGSARKISTDGSFDSSGEDELAIQRLETTKNELRQRIAKEARGNAILQASLERRK 614

Query: 633 KILQERRLTLEQEVARLREQLQREKD----------------PSANISEKTKADLGESNR 692
           + L ERRL+LEQ+V+RL+EQLQ E+D                 S  +  KT+A+L E   
Sbjct: 615 QALHERRLSLEQDVSRLQEQLQAERDLRAALEVGLSMSSGQFSSHGVDSKTRAELEEIAL 674

Query: 693 GEKDTANINNTMDEFVGLLNRIRDQK-SNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAP- 752
            E D A +   + E    LN+ R     +  D R+  Q +QNH  + +  ++D  S    
Sbjct: 675 AEADVARLKQKVAELHHQLNQQRQTHFGSFSDARDTHQYLQNHNPQKRFLQQDFDSTLAY 734

Query: 753 -SHPEHSRNKDVLSG-----------------HAEISKHSPLHQHLDPHNPSAKIGVLAT 788
            +H    R+++ + G                      K  P   +      S + G ++ 
Sbjct: 735 VNHERKQRHEENVLGAEWRNSKGAGSFGVGNSRQPSRKQIPESTNTTDSKISEESGKISV 794

BLAST of CmaCh12G001900 vs. TAIR 10
Match: AT5G19390.2 (Rho GTPase activation protein (RhoGAP) with PH domain )

HSP 1 Score: 593.2 bits (1528), Expect = 3.3e-169
Identity = 389/842 (46.20%), Postives = 511/842 (60.69%), Query Frame = 0

Query: 33  AGNTVFKSGPLFLSSKGIGWTSWKKRWFILTRTSLVFFRSDPNAVPQKGGEANLTLGGID 92
           A NTVFKSGPLF+SSKG+GWTSWKKRWFILTRTSLVFF++DP  +PQKGGE NLTLGGID
Sbjct: 15  ASNTVFKSGPLFISSKGLGWTSWKKRWFILTRTSLVFFKNDPGTLPQKGGEVNLTLGGID 74

Query: 93  LNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEWKAALENALAQAPSSA--- 152
           LNNSGSVVV+ +KKLLTVLFPDGR+GRAFTLKAET EDLYEWK ALE ALAQAP++A   
Sbjct: 75  LNNSGSVVVREDKKLLTVLFPDGRDGRAFTLKAETFEDLYEWKTALEQALAQAPNAALIM 134

Query: 153 NANGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDVDGTPSFLEKALRFIE 212
             NGI + +       ++E ++  + +P +  V+GRPILLALED+DG+PSFLEKAL+FIE
Sbjct: 135 GQNGIFRAE-------TNEAIEGREKRPLKSLVVGRPILLALEDIDGSPSFLEKALQFIE 194

Query: 213 THGVKVEGILRQAADVDDVEHRLRGYEQGKIEFSPEEDAHVVGDCVKYVIRELPSSPVPA 272
            +G K+EGILRQ+ADV++VE R++ YEQGK EF+ +ED HVVGDC+K+V+RELPSSPV A
Sbjct: 195 KYGTKIEGILRQSADVEEVERRVQEYEQGKTEFTFDEDPHVVGDCIKHVLRELPSSPVSA 254

Query: 273 SCCNALLEASKTE-RGGRVNAMRSAIYETFPEPNRRLLQRILMMMQAVASHKAENRMSSS 332
           SCC ALLEA + E +  R++++RSAI ETFPEPNRRLLQRIL MM  ++SH  ENRM+ +
Sbjct: 255 SCCTALLEAYRIESKEARISSLRSAIAETFPEPNRRLLQRILKMMHTISSHSHENRMNPN 314

Query: 333 AVAACMAPLLLRPLLAGECEIETDFDVGGDGSIQLLRAAAAANHAQAIVITLLEEYGKIF 392
           AVAACMAPLLLRPLLAGEC++E DFD G D S QLL AA AAN+AQAI+  LLE+YG IF
Sbjct: 315 AVAACMAPLLLRPLLAGECDLEDDFDGGEDNSAQLLAAANAANNAQAIITVLLEDYGSIF 374

Query: 393 GEGSMSPI-LYSDSEESGSESEEASDDDMSYDDEEQDDATGSDVATDDELESA--GTCSG 452
            E ++    + ++S    S  +++SDDD +  +   +     +  TDD+ + A  G  S 
Sbjct: 375 DEENIQRCSISTESHIGNSGPDDSSDDDNNMKNGYHNADNEVEPVTDDDNDRALSGKMSE 434

Query: 453 SVGSGEHDLYDDKG-----SDVLSTSSKNSDMCDVNGMKPKSSKERIHDKIDRGR----G 512
           S G    DLY+ KG     SD+ S    N   C+ N       +    +  D+      G
Sbjct: 435 SSGCTGSDLYEYKGFVADDSDIESPRDTNGPRCNSNIRTDHLMRNPFVNSTDQQAGEQIG 494

Query: 513 NSPIKDEKSCEVECASESFEGS------PTTSN-------------------KSSNTTKR 572
           + P K   +  +   SES++ S      PT  N                    SS   KR
Sbjct: 495 DDPTKYGVNSCLAHVSESYQQSGTGLNVPTHGNTLAAPGLESPSAKSVNKGTPSSVHAKR 554

Query: 573 LTIWGRTPAKKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRK 632
            T WGR  A+K  +  S D    +E+ IQRLE TK EL+ +I + AR NA+LQ+SLE RK
Sbjct: 555 ATFWGRGSARKISTDGSFDSSGEDELAIQRLETTKNELRQRIAKEARGNAILQASLERRK 614

Query: 633 KILQERRLTLEQEVARLREQLQREKD----------------PSANISEKTKADLGESNR 692
           + L ERRL+LEQ+V+RL+EQLQ E+D                 S  +  KT+A+L E   
Sbjct: 615 QALHERRLSLEQDVSRLQEQLQAERDLRAALEVGLSMSSGQFSSHGVDSKTRAELEEIAL 674

Query: 693 GEKDTANINNTMDEFVGLLNRIRDQK-SNLPDLRNVSQQMQNHQTKLKDKRKDAGSLAP- 752
            E D A +   + E    LN+ R     +  D R+  Q +QNH  + +  ++D  S    
Sbjct: 675 AEADVARLKQKVAELHHQLNQQRQTHFGSFSDARDTHQYLQNHNPQKRFLQQDFDSTLAY 734

Query: 753 -SHPEHSRNKDVLSG-----------------HAEISKHSPLHQHLDPHNPSAKIGVLAT 788
            +H    R+++ + G                      K  P   +      S + G ++ 
Sbjct: 735 VNHERKQRHEENVLGAEWRNSKGAGSFGVGNSRQPSRKQIPESTNTTDSKISEESGKISV 794

BLAST of CmaCh12G001900 vs. TAIR 10
Match: AT5G12150.1 (Rho GTPase activation protein (RhoGAP) with PH domain )

HSP 1 Score: 586.6 bits (1511), Expect = 3.1e-167
Identity = 388/823 (47.14%), Postives = 510/823 (61.97%), Query Frame = 0

Query: 33  AGNTVFKSGPLFLSSKGIGWTSWKKRWFILTRTSLVFFRSDPNAVPQKGGEANLTLGGID 92
           A NTV+KSGPLF+SSKG+GWTSWKKRWFILTRTSLVFF++DP+A+PQKGGE NLTLGGID
Sbjct: 15  ASNTVYKSGPLFISSKGLGWTSWKKRWFILTRTSLVFFKNDPSALPQKGGEVNLTLGGID 74

Query: 93  LNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEWKAALENALAQAPSSA--- 152
           LN+SGSVVV+ +KKLLTVLFPDGR+GRAFTLKAETL+DLYEWKAALE ALAQAP++A   
Sbjct: 75  LNSSGSVVVREDKKLLTVLFPDGRDGRAFTLKAETLDDLYEWKAALEQALAQAPNAALVI 134

Query: 153 NANGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDVDGTPSFLEKALRFIE 212
             NGI + +       S  + +D +P   +  V+GRPILLALE++DG+PSFLEKAL+F+E
Sbjct: 135 GQNGIFRTEANNTIEASFNSWRDQRPL--KSSVVGRPILLALEEIDGSPSFLEKALQFLE 194

Query: 213 THGVKVEGILRQAADVDDVEHRLRGYEQGKIEFSPEEDAHVVGDCVKYVIRELPSSPVPA 272
           T+G KVEGILRQ+ADV++VE R++ YEQGK EFSPEED HVVGDCVK+V+R+LPSSPVPA
Sbjct: 195 TYGTKVEGILRQSADVEEVERRVQEYEQGKTEFSPEEDPHVVGDCVKHVLRQLPSSPVPA 254

Query: 273 SCCNALLEASKTERG-GRVNAMRSAIYETFPEPNRRLLQRILMMMQAVASHKAENRMSSS 332
           SCC ALLEA K ++   RVN++RSAI ETFPEPNRRLL R+L MM  + SH +ENRM+SS
Sbjct: 255 SCCTALLEAYKIDQNEARVNSLRSAIIETFPEPNRRLLLRMLKMMHTITSHSSENRMTSS 314

Query: 333 AVAACMAPLLLRPLLAGECEIETDFDVGGDGSIQLLRAAAAANHAQAIVITLLEEYGKIF 392
           AVAACM+PLLLRPLLAGEC++E  FD  GD S QLL AA AAN+AQAIV  LLE+YG + 
Sbjct: 315 AVAACMSPLLLRPLLAGECDLE-GFDTLGDNSAQLLAAANAANNAQAIVTALLEDYGNMI 374

Query: 393 GEGSMSPILYSDSEESGSESEEASDDD----MSYDDEEQDDATGSDVATDDELESAGTCS 452
            +  +     S     G    E S D+    + + D    D    +   D+++  +   S
Sbjct: 375 NDEGLGRCSTSTDSHIGDSGPENSSDEEEIVVKHPDLHTLDIEEGETDDDNDVLLSRKPS 434

Query: 453 GSVGSGEHDLYDDKGSDVLSTSSKNSDMCDVN-GMKPKSSKERIHDKIDRGRGNSPIKDE 512
            S      DLYD KG  V  + +++    D++  ++      R+   I+      PIKD 
Sbjct: 435 ESSDYAGSDLYDYKGFGVEDSDAESPR--DIHCSVESTDFSARVKKHIE-----EPIKDI 494

Query: 513 KSCEV----ECASESFEGSPT----------------------TSNKSSNTTKRLTIWGR 572
           +   V     C     E  P+                      T+  S+  +KR + WGR
Sbjct: 495 EVSSVSPTENCYQSGREAIPSVTPSTPLTALRYTTSAEKPASKTTGSSTVNSKRSSSWGR 554

Query: 573 TPAKKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRKKILQER 632
              KK  +  S D    +E+ IQRLE  K EL+ +I + A+ NA LQ+SLE RK+ L ER
Sbjct: 555 GNGKKTPAKGSFDSSGNDELLIQRLEHMKDELRQRIAKEAKGNAALQASLERRKQALHER 614

Query: 633 RLTLEQEVARLREQLQREKD----------------PSANISEKTKADLGESNRGEKDTA 692
           RL LEQ+V RL+EQLQ E+D                 S     KT+A+L E    E D A
Sbjct: 615 RLALEQDVGRLQEQLQAERDLRSALEVGLSISCGQFSSQAADSKTRAELEEIALAEADVA 674

Query: 693 NINNTMDEFVGLLNRIRDQK-SNLPDLRNVSQQMQNHQTKLKDKRKDAGS-LAPSHPEHS 752
            +   + E    L++ R    S+LPD ++  Q + NH T+LK  ++D  S LA  + E +
Sbjct: 675 RLKQKVAELHHQLSQQRQHHLSSLPDAQSHHQFLHNHNTQLKSFQQDFDSILAFVNHERN 734

Query: 753 RNKDVLSGHAEISKHSPLHQHLDPHNPS---AKIGVLATNFATGLTNPKRTGARMELGPN 796
           +  D  S  A+       ++ + P +PS   A +G+    ++            M+ G +
Sbjct: 735 QRTDETSLRADWRNGRGNNRQV-PGSPSLNAASLGIPMEEYS----------PVMDYGRH 794

BLAST of CmaCh12G001900 vs. TAIR 10
Match: AT5G19390.4 (Rho GTPase activation protein (RhoGAP) with PH domain )

HSP 1 Score: 576.2 bits (1484), Expect = 4.2e-164
Identity = 357/703 (50.78%), Postives = 456/703 (64.86%), Query Frame = 0

Query: 33  AGNTVFKSGPLFLSSKGIGWTSWKKRWFILTRTSLVFFRSDPNAVPQKGGEANLTLGGID 92
           A NTVFKSGPLF+SSKG+GWTSWKKRWFILTRTSLVFF++DP  +PQKGGE NLTLGGID
Sbjct: 15  ASNTVFKSGPLFISSKGLGWTSWKKRWFILTRTSLVFFKNDPGTLPQKGGEVNLTLGGID 74

Query: 93  LNNSGSVVVKSEKKLLTVLFPDGREGRAFTLKAETLEDLYEWKAALENALAQAPSSA--- 152
           LNNSGSVVV+ +KKLLTVLFPDGR+GRAFTLKAET EDLYEWK ALE ALAQAP++A   
Sbjct: 75  LNNSGSVVVREDKKLLTVLFPDGRDGRAFTLKAETFEDLYEWKTALEQALAQAPNAALIM 134

Query: 153 NANGILKNDKMEPNNGSSETLKDGQPQPGRPRVLGRPILLALEDVDGTPSFLEKALRFIE 212
             NGI + +       ++E ++  + +P +  V+GRPILLALED+DG+PSFLEKAL+FIE
Sbjct: 135 GQNGIFRAE-------TNEAIEGREKRPLKSLVVGRPILLALEDIDGSPSFLEKALQFIE 194

Query: 213 THGVKVEGILRQAADVDDVEHRLRGYEQGKIEFSPEEDAHVVGDCVKYVIRELPSSPVPA 272
            +G K+EGILRQ+ADV++VE R++ YEQGK EF+ +ED HVVGDC+K+V+RELPSSPV A
Sbjct: 195 KYGTKIEGILRQSADVEEVERRVQEYEQGKTEFTFDEDPHVVGDCIKHVLRELPSSPVSA 254

Query: 273 SCCNALLEASKTE-RGGRVNAMRSAIYETFPEPNRRLLQRILMMMQAVASHKAENRMSSS 332
           SCC ALLEA + E +  R++++RSAI ETFPEPNRRLLQRIL MM  ++SH  ENRM+ +
Sbjct: 255 SCCTALLEAYRIESKEARISSLRSAIAETFPEPNRRLLQRILKMMHTISSHSHENRMNPN 314

Query: 333 AVAACMAPLLLRPLLAGECEIETDFDVGGDGSIQLLRAAAAANHAQAIVITLLEEYGKIF 392
           AVAACMAPLLLRPLLAGEC++E DFD G D S QLL AA AAN+AQAI+  LLE+YG IF
Sbjct: 315 AVAACMAPLLLRPLLAGECDLEDDFDGGEDNSAQLLAAANAANNAQAIITVLLEDYGSIF 374

Query: 393 GEGSMSPI-LYSDSEESGSESEEASDDDMSYDDEEQDDATGSDVATDDELESA--GTCSG 452
            E ++    + ++S    S  +++SDDD +  +   +     +  TDD+ + A  G  S 
Sbjct: 375 DEENIQRCSISTESHIGNSGPDDSSDDDNNMKNGYHNADNEVEPVTDDDNDRALSGKMSE 434

Query: 453 SVGSGEHDLYDDKG-----SDVLSTSSKNSDMCDVNGMKPKSSKERIHDKIDRGR----G 512
           S G    DLY+ KG     SD+ S    N   C+ N       +    +  D+      G
Sbjct: 435 SSGCTGSDLYEYKGFVADDSDIESPRDTNGPRCNSNIRTDHLMRNPFVNSTDQQAGEQIG 494

Query: 513 NSPIKDEKSCEVECASESFEGS------PTTSN-------------------KSSNTTKR 572
           + P K   +  +   SES++ S      PT  N                    SS   KR
Sbjct: 495 DDPTKYGVNSCLAHVSESYQQSGTGLNVPTHGNTLAAPGLESPSAKSVNKGTPSSVHAKR 554

Query: 573 LTIWGRTPAKKKLSLESIDYDFGEEVEIQRLEATKCELQNKIVEAARENAVLQSSLENRK 632
            T WGR  A+K  +  S D    +E+ IQRLE TK EL+ +I + AR NA+LQ+SLE RK
Sbjct: 555 ATFWGRGSARKISTDGSFDSSGEDELAIQRLETTKNELRQRIAKEARGNAILQASLERRK 614

Query: 633 KILQERRLTLEQEVARLREQLQREKD----------------PSANISEKTKADLGESNR 678
           + L ERRL+LEQ+V+RL+EQLQ E+D                 S  +  KT+A+L E   
Sbjct: 615 QALHERRLSLEQDVSRLQEQLQAERDLRAALEVGLSMSSGQFSSHGVDSKTRAELEEIAL 674