CmaCh12G001830 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G001830
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionSerine hydroxymethyltransferase
LocationCma_Chr12: 838635 .. 844246 (-)
RNA-Seq ExpressionCmaCh12G001830
SyntenyCmaCh12G001830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACATATACCTCGAAAAAAAATTATTAAAAAATAGCTTAGTTAATATTTTTTCACCGGCATCGGGCCTCCATCACCAACCTACTAACCCCACTGGCAGTGGTGACAACGGAAGAGCACCAGATCAAAAGTGCGCGGAACCTTTCACGCATTTCGATATCGGGAGGAGATTCAATCGCTTACAGAAACCAATGGCTATGGCGCTTCGAAGACTCGCCGTCGGCGTTACCAAATCCAATGTTCCTCTCGTCAATGGCCGGTTTCTCCATCACATGGTAATTCTCACCGCCATTGTTAATTCATAATATCTATGGTGTTTACATTCGTCATTCTTGATAACTTCTGATGCCGAAATTTTCTTACTCGTTTTTGTGTATGAATTCGTTTTATATGGCCGCAGTCTTCTCTGTCCGGTGCCGCTGCGGAGGAAAGGGACAAGGCTCGAGCTACTGTGAGCATTCCATTTATTTATATCTAAAGAAATTTACATTTTCTGATGCAAAAATTTCTTATTGAAGTTAGAACTTAAGTAAGGACTAAGGAATGTATGTGGATCATGGAGTGTACGAGATCTGTTTGATGACTCAGTTTCTTGTATCGATGTTGATATTTGGAGGAAATTTTGCTTGCAGTGGATTAAGCAGCTGAACGCCCCCCTCGGGGTTATTGATCCGGAAATTGCAGACATCATTGAGCTCGAGAAAGCTCGTCAATGGAAGGTATTTTTTGTTTCACTACGAGCTGTTTTCGTGCTCTTTGTGCTTTTGAAAATTTGACGTTGATCTATTCGCTTCATTTTTTTTAAGGTTGAAATTGTCGTTAATGGTGTTGATATCCATTGAATTGAGTTGAGCTTCGTGATTAACATCAAATCTTGTACGATTCTTTTTTGAATGTGAAGGGTTTTGAACTTATACCTTCTGAGAATTTCACTTCATCATCAGTGATGGAAGCAGTTGGTTCAGTCATGACCAACAAGTACAGTGAAGGATATCCTGGTGCCAGATACTATGGTGGAAATGAGTATGTGGATCTATCTCAATTCCAGAGTACTTTTGTTTTTGGAATAAATTGTTGAAGTTTTGAAACAAAATAAGAACAGAGTATAAATTACATGAAAGACCATCATCAGTTTTTCTTAATATTAGCAAAAACGTTGCAAGTTCTTAGAATCTGTAACTTACTGCGATGGCACCGATAATTTCTGTATGTAATTCCTTTTGTTTTCATTTTTCTCTTTTTTCTTTTCGTTTCCTTTATGGGCTTCTAATTATAAACGCCAAATGGATTTAGGTACATTGACATGGCTGAAACCTTGTGCCAGAAACGTGCACTAGAAGCTTTCCGTTTGGACCCTGCAAAATGGGGAGGTATGAGAATTATTGATATATTATTGTTGTTATTCTTTTTTGAAGTATTACTATGCAGGGCGGGGGATTGGACCTTCGGCTTCTAGGCAGTTAACAGCTGCGTTTATCTACTTAGTTATGCTTTGATAGACAATTAATGATATGTGATTAATTTCCAAGTGGTAAATTTGTTTCAGACTCTCTTTGTATAAGTATAGCAGTTCACTGTGTCTTGTCCTGAGTTTCGTGTTAATTCAGCTGAACACGTGCAATCGTTTTAAGAACTTTCTGATTCAGTAAATGTACTTATAAACAAGCTTTTCTCGACATGGTGCCTTTTAATGGTGTGATCAATGAAAAATAAAAGACCCACGGGATGGCTGTTGCATATATTAATCAGAAAATTGTCTGCACCTCCCTTTTCTTTTGGACACATCTTGGAAAGAAGACATGTTGGTGATATTTGCTCTAGTGTGAACCACTGAAGTCTCGTGGTTTGGTTTGGCTTTCTCTTTTTATATAACCAACTCTGAGACCATTCAGTTTGGTCTATGTGAGAGAACTTGCCAAAGTACCTACCGTCTTTGATTAAACCCAGAATGGTATTTTTTTTGTAGTTAATGTGCAGTCTCTCTCTGGATCACCGGCTAACTTTCAGGCATATACTGCATTATTAAAACCTCATGAGAGGATTATGGCTCTAGATTTACCACATGGTGGACACCTTTCGCATGGTTACCAGGTGTTTATTTTCAAATTTACTACTTGGTTTTGACATTGCTGCTTTTGGTGATTTGGTAACTTGTACTTTTTATGATGTTTGATACGATACTTTCCTATGTTTATTTCTTTAACTTTTTGCAGACTGATACCAAGAAGATTTCTGCCGTTTCAATATTCTTCGAGACAGTGCCATATAGGTTGGATGAGAGCACTGGTTACATTGACTATGACCAGGTTCGAAATGCTTCTGTGTTCGCGCCTATTGGGATATCCTGTTGATTTGTCTTGAAACTGCAATTTCAAATAAACTAACTTATATGGAGCAAAATTCTAATAGTTGGAAAAAAGTGCATCATTGTTCCGACCAAAACTCATCGTTGCTGGTGCAAGTGCCTACGCCCGTCTCTATGACTATGCACGAATTCGCCAGGTTTTTCCTTTGTTTGGCGATAGATTATAAAATAGCTTCCATTAGGATTTTACACTTCTACGTTGTGCAACATTTGACCTAGTTCTGATTATTTTGATTTCTGACAGATCTGTGACAAACAGAAGGCAGTCATGTTGGCTGATATGGCACACATTAGTGGCTTAGTTGCAGCTGGCGTTATTCCATCCCCTTTTGAGTATGCTGATATTGTGACAACAACCACGCATAAATCCTTACGCGGACCACGAGGGGCAATGATTTTCTTCCGGAAGGGTGTTAAAGAGATCAACAAAAAAGGACAAGAAGTAAGTAAAGGTTCCAACTACGCCTTGAAGTGAATTTAAGTAAATTTCAAGGGCAAGTTCTTACTGGTCTAATATTTCATTATAATCTATGACAGGTGCTCTATGACTACGAAGACAAAATTAATCAGGCTGTCTTTCCTGGTCTTCAAGGTGGCCCACACAACCACACCATAACTGGTCTGGCAGTTGCACTAAAGCAGGTCGTTTATTTTTTCTTGTCTTTCTCGACAAATAAAGTTGGGGGCCATCTTCACGAAATTTTATTTCGGATACTAGATTTAGATATTTGGATGTCCGCCTAGTTTCCATTCATGATATTTCTCCTCTTTACCATATTGCAATAGGCCACAACTCCGGAATACAAAGCCTACCAAGAGCAGGTTCTTAGTAATTCTTCCAAATTTGCTCAGGTATGCAGTGAAAATTTTCTGACTCCTTTGACAAATCATCAATATGGTACTTTTTCATTGCATGTTTTGCTTGCTGTAAGGAGTAATTAATTCCTGAACTTTCTTCTTTGCTTAAATCAGTGGAGTTTACCGTGTCAAATCAATTGTTTATGCAGAGTTTGATTGAGAAGGGCTATGAACTTGTATCTGGTGGAACTGACAACCATTTAGTCCTTGTGAATTTGAAAAATAAGGTAAAGGATGACAACCTGTCCGCCAATATAGCTATTATACTCCTCATCCGTGGGTTTGTGTACTCTCTTCAGGTGTTTGTGTTTGAGTAGTGCTGGGTGCAAACAATTACAAATATAATGACAATTTAATTAACAAAAATCATCCCGAGTTATTTCCATTGTTCAAACTTATGATATAAATGTTATCCTTGGCTTGATCTGGACAAAACTTTAGAAATGACTTGCAGCACTGGTGTACAATCCTTTTCTTATTGTTTTATTTGTTTTATTTATTTATTTATTTTTTTTTTTTTNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNNGGGGGGGGGGGGGGGGGGGGGGGGTGTCATTCGGCAGGGTATCGATGGCTCGAGAGTCGAGAAGGTTTTGGAATCGGTTCATATTGCAGCTAACAAGAACACAGTTCCAGGGGACGTGTCTGCCATGGTTCCTGGTGGCATCCGTATGGGTATGGCCTTCCACCATTGTCTTTGGTCCTTCAATTACTGGTAGAATATCTAAATTTATGCCAATTTTGTGTTCTGAACTTCTGGTGTACATAGGGACCCCTGCCCTGACCTCAAGAGGTTTCCTTGAGGCAGATTTTGTGAAAGTAGCTGAATATTTCGATGAAGCTGTGAAGTTAGCCTTGAAGATCAAGGCCAATAGCGAAGGTTCCTGAACAAAATACTTCTCTACCTTAAAATATACTTTCCATTCTCGTCTCGTATTGCCCATTTATCGGACTAATGTCTTTTAGAACCTTCGATATACTCATCTTTGTTTTTAAATATTGTAAATACAGGTACTAAGTTGAAGGATTTCGTAGCAACCATGCAATCAAATAAAGACTTCCAATCTGAAATTTCAAAGCTCCGACTCAAAGTTGAGCAGTATGCCAAGCAATTTCCAACAATAGGATTTGAGAAAGAGACAATGAAATACAGAGACTGAAAAGCGAATCCTCAAAGTTGCAGGCAAGGATCTACCGCGGAGTCGGGTTAGAACATTTCTTAACATCCATAACCTGTTGAAAGAATACCGTAAATAACTTACAAGTTCTAACAAATAATTGGTTAGGGTGTCTTGATGATCTTTATGAGATGAAAAGTTGTTTTAGTAATCACCCTTATAAGGGTGTGGAAACCTTTCCCTAGTAGACACATTTTAAAGCCTTGAGGGGAAGCCTGAAACGGAAAGCCTAAAGAGGACTATATTTGCTAGCGGTAGGCCTGAATCGTTACAAGTGGTATCAGAGCCAAACACCGGACGATGTGTCAGCCTTCTCGTTGTTCCCCGACGGGGGATAGACACGAGGCGGTGTGCCAGTAAGGATGCTGGACCCCAAAGGGGGTGGATTTGGTGGCGGTCCCACATCGATTGAAGAAAGGAACAAGTGCCAGCGAGGACGCTGAGCCCCAAATGGGGGTGAATTGTGATGTCCCACATTGGTTGGGGAGGAGAATAAAACATCCTTTATAAGGGTGTGGAAATCTTCTCCTAATAGACGCGTTTTAAAACCTTGAGAGGAAGTCTGAAAGGGAAAGTCCAAAGAGGACAATATCTGCTAGCGATGGGCATGGGCCTGAGTCGTTAGAGTATGAAGAACACTTCTGAAAATCTGTTTAGATAATTAAAATGATATGGAAAAGCCTAAAAATACTCGTCTAGTGATTTGATTCTTCAAGTTTTAGAGTGTCATAATTCTGGGTTAGATTGATATTTGATATTCTATTAAGCAGCATCCATACTTCTGCTTATGCAGATAACCTTGTTGTCAAAGTTGCTGATTCAGACCCTCCAGCAAACGGCCAACTGCAGAAGTTTCCTCTAAATAAATGTCCTTGTGAGCAATTTTTTGTTTGACTTTCTAGTATAATAAAATCTCGACATTATTCTCTTTCGCATGCTTTAATTTATACGTTCACATCTTAATGTAAACATAATGTGATAAGGAATATCTCAAAGGAAGAACACTTCACAAGTAAGATTGTGACTATGTTTTCTTTTTGGAGACAATGATCGTCGATTATGTGCGTAGTTATTTGAATTTTAGTTTGAACCATGAGTTTATTTTCGCCTAAAACTTTCCACTAATGACCA

mRNA sequence

ACATATACCTCGAAAAAAAATTATTAAAAAATAGCTTAGTTAATATTTTTTCACCGGCATCGGGCCTCCATCACCAACCTACTAACCCCACTGGCAGTGGTGACAACGGAAGAGCACCAGATCAAAAGTGCGCGGAACCTTTCACGCATTTCGATATCGGGAGGAGATTCAATCGCTTACAGAAACCAATGGCTATGGCGCTTCGAAGACTCGCCGTCGGCGTTACCAAATCCAATGTTCCTCTCGTCAATGGCCGGTTTCTCCATCACATGTCTTCTCTGTCCGGTGCCGCTGCGGAGGAAAGGGACAAGGCTCGAGCTACTTGGATTAAGCAGCTGAACGCCCCCCTCGGGGTTATTGATCCGGAAATTGCAGACATCATTGAGCTCGAGAAAGCTCGTCAATGGAAGGGTTTTGAACTTATACCTTCTGAGAATTTCACTTCATCATCAGTGATGGAAGCAGTTGGTTCAGTCATGACCAACAAGTACAGTGAAGGATATCCTGGTGCCAGATACTATGGTGGAAATGAGTACATTGACATGGCTGAAACCTTGTGCCAGAAACGTGCACTAGAAGCTTTCCGTTTGGACCCTGCAAAATGGGGAGTTAATGTGCAGTCTCTCTCTGGATCACCGGCTAACTTTCAGGCATATACTGCATTATTAAAACCTCATGAGAGGATTATGGCTCTAGATTTACCACATGGTGGACACCTTTCGCATGGTTACCAGACTGATACCAAGAAGATTTCTGCCGTTTCAATATTCTTCGAGACAGTGCCATATAGGTTGGATGAGAGCACTGGTTACATTGACTATGACCAGTTGGAAAAAAGTGCATCATTGTTCCGACCAAAACTCATCGTTGCTGGTGCAAGTGCCTACGCCCGTCTCTATGACTATGCACGAATTCGCCAGATCTGTGACAAACAGAAGGCAGTCATGTTGGCTGATATGGCACACATTAGTGGCTTAGTTGCAGCTGGCGTTATTCCATCCCCTTTTGAGTATGCTGATATTGTGACAACAACCACGCATAAATCCTTACGCGGACCACGAGGGGCAATGATTTTCTTCCGGAAGGGTGTTAAAGAGATCAACAAAAAAGGACAAGAAGTGCTCTATGACTACGAAGACAAAATTAATCAGGCTGTCTTTCCTGGTCTTCAAGGTGGCCCACACAACCACACCATAACTGGTCTGGCAGTTGCACTAAAGCAGGCCACAACTCCGGAATACAAAGCCTACCAAGAGCAGGTTCTTAGTAATTCTTCCAAATTTGCTCAGAGTTTGATTGAGAAGGGCTATGAACTTGTATCTGGTGGAACTGACAACCATTTAGTCCTTGTGAATTTGAAAAATAAGGGTATCGATGGCTCGAGAGTCGAGAAGGTTTTGGAATCGGTTCATATTGCAGCTAACAAGAACACAGTTCCAGGGGACGTGTCTGCCATGGTTCCTGGTGGCATCCGTATGGGGACCCCTGCCCTGACCTCAAGAGGTTTCCTTGAGGCAGATTTTGTGAAAGTAGCTGAATATTTCGATGAAGCTGTGAAGTTAGCCTTGAAGATCAAGGCCAATAGCGAAGGTACTAAGTTGAAGGATTTCGTAGCAACCATGCAATCAAATAAAGACTTCCAATCTGAAATTTCAAAGCTCCGACTCAAAGTTGAGCAGTATGCCAAGCAATTTCCAACAATAGGATTTGAGAAAGAGACAATGAAATACAGAGACTGAAAAGCGAATCCTCAAAGTTGCAGGCAAGGATCTACCGCGGAGTCGGATAACCTTGTTGTCAAAGTTGCTGATTCAGACCCTCCAGCAAACGGCCAACTGCAGAAGTTTCCTCTAAATAAATGTCCTTGTGAGCAATTTTTTGTTTGACTTTCTAGTATAATAAAATCTCGACATTATTCTCTTTCGCATGCTTTAATTTATACGTTCACATCTTAATGTAAACATAATGTGATAAGGAATATCTCAAAGGAAGAACACTTCACAAGTAAGATTGTGACTATGTTTTCTTTTTGGAGACAATGATCGTCGATTATGTGCGTAGTTATTTGAATTTTAGTTTGAACCATGAGTTTATTTTCGCCTAAAACTTTCCACTAATGACCA

Coding sequence (CDS)

ATGGCTATGGCGCTTCGAAGACTCGCCGTCGGCGTTACCAAATCCAATGTTCCTCTCGTCAATGGCCGGTTTCTCCATCACATGTCTTCTCTGTCCGGTGCCGCTGCGGAGGAAAGGGACAAGGCTCGAGCTACTTGGATTAAGCAGCTGAACGCCCCCCTCGGGGTTATTGATCCGGAAATTGCAGACATCATTGAGCTCGAGAAAGCTCGTCAATGGAAGGGTTTTGAACTTATACCTTCTGAGAATTTCACTTCATCATCAGTGATGGAAGCAGTTGGTTCAGTCATGACCAACAAGTACAGTGAAGGATATCCTGGTGCCAGATACTATGGTGGAAATGAGTACATTGACATGGCTGAAACCTTGTGCCAGAAACGTGCACTAGAAGCTTTCCGTTTGGACCCTGCAAAATGGGGAGTTAATGTGCAGTCTCTCTCTGGATCACCGGCTAACTTTCAGGCATATACTGCATTATTAAAACCTCATGAGAGGATTATGGCTCTAGATTTACCACATGGTGGACACCTTTCGCATGGTTACCAGACTGATACCAAGAAGATTTCTGCCGTTTCAATATTCTTCGAGACAGTGCCATATAGGTTGGATGAGAGCACTGGTTACATTGACTATGACCAGTTGGAAAAAAGTGCATCATTGTTCCGACCAAAACTCATCGTTGCTGGTGCAAGTGCCTACGCCCGTCTCTATGACTATGCACGAATTCGCCAGATCTGTGACAAACAGAAGGCAGTCATGTTGGCTGATATGGCACACATTAGTGGCTTAGTTGCAGCTGGCGTTATTCCATCCCCTTTTGAGTATGCTGATATTGTGACAACAACCACGCATAAATCCTTACGCGGACCACGAGGGGCAATGATTTTCTTCCGGAAGGGTGTTAAAGAGATCAACAAAAAAGGACAAGAAGTGCTCTATGACTACGAAGACAAAATTAATCAGGCTGTCTTTCCTGGTCTTCAAGGTGGCCCACACAACCACACCATAACTGGTCTGGCAGTTGCACTAAAGCAGGCCACAACTCCGGAATACAAAGCCTACCAAGAGCAGGTTCTTAGTAATTCTTCCAAATTTGCTCAGAGTTTGATTGAGAAGGGCTATGAACTTGTATCTGGTGGAACTGACAACCATTTAGTCCTTGTGAATTTGAAAAATAAGGGTATCGATGGCTCGAGAGTCGAGAAGGTTTTGGAATCGGTTCATATTGCAGCTAACAAGAACACAGTTCCAGGGGACGTGTCTGCCATGGTTCCTGGTGGCATCCGTATGGGGACCCCTGCCCTGACCTCAAGAGGTTTCCTTGAGGCAGATTTTGTGAAAGTAGCTGAATATTTCGATGAAGCTGTGAAGTTAGCCTTGAAGATCAAGGCCAATAGCGAAGGTACTAAGTTGAAGGATTTCGTAGCAACCATGCAATCAAATAAAGACTTCCAATCTGAAATTTCAAAGCTCCGACTCAAAGTTGAGCAGTATGCCAAGCAATTTCCAACAATAGGATTTGAGAAAGAGACAATGAAATACAGAGACTGA

Protein sequence

MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPEIADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYARIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLSNSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQSNKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD
Homology
BLAST of CmaCh12G001830 vs. ExPASy Swiss-Prot
Match: Q94C74 (Serine hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM2 PE=1 SV=2)

HSP 1 Score: 890.2 bits (2299), Expect = 1.1e-257
Identity = 445/517 (86.07%), Postives = 482/517 (93.23%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKS-NVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDP 60
           MA+ALRRL+  V K  ++   NG  L  MSSLS AA  E +K+R++WIKQLNA L  IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  EIADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM 120
           E+ADIIELEKARQWKGFELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 AETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH 180
           AETLCQKRALEAF+LDP+KWGVNVQSLSGSPANFQ YTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDY 240
           GYQTDTKKISAVSIFFET+PYRLDE+TGYIDYDQLEKSA LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 300
           ARIR++C+KQKAVMLADMAHISGLVAAGVIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 360
           G+KEINK+G+EV+YDYED+INQAVFPGLQGGPHNHTITGLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 SNSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGD 420
            N SKFA++L+ KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKVLE VHIAANKNTVPGD
Sbjct: 361 RNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGD 420

Query: 421 VSAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQ 480
           VSAMVPGGIRMGTPALTSRGF+E DF KVAEYFD AVK+ALKIKA S+GTKLKDFVATMQ
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLKDFVATMQ 480

Query: 481 SNKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           SN+  QSE+SKLR  VE+YAKQFPTIGFEKETM+Y++
Sbjct: 481 SNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYKE 517

BLAST of CmaCh12G001830 vs. ExPASy Swiss-Prot
Match: P50433 (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 886.7 bits (2290), Expect = 1.2e-256
Identity = 438/516 (84.88%), Postives = 480/516 (93.02%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MA+ALRRL+  V K    L NG  L++MSSL   A  +++K+   W KQLNAPL V+DPE
Sbjct: 3   MAIALRRLSATVDKPVKSLYNGGSLYYMSSLPNEAVYDKEKSGVAWPKQLNAPLEVVDPE 62

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIE EKARQWKG ELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 63  IADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMA 122

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ETLCQKRALEAFRLDPAKWGVNVQ LSGSPANFQ YTALLKPHERIMALDLPHGGHLSHG
Sbjct: 123 ETLCQKRALEAFRLDPAKWGVNVQPLSGSPANFQVYTALLKPHERIMALDLPHGGHLSHG 182

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFET+PYRLDESTGYIDYDQLEKSA+LFRPKLIVAGASAYARLYDY 
Sbjct: 183 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYD 242

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIR++C+KQKA++LADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIF+RKG
Sbjct: 243 RIRKVCNKQKAILLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFYRKG 302

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKE+NK+G+EV YDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEY+AYQEQVLS
Sbjct: 303 VKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQVLS 362

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NSSKFAQ+L EKGYELVSGGTDNHLVLVN+KNKGIDGSRVEKVLE+VHIAANKNTVPGDV
Sbjct: 363 NSSKFAQALGEKGYELVSGGTDNHLVLVNMKNKGIDGSRVEKVLEAVHIAANKNTVPGDV 422

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLE DFVKVA++FD AVK+A+K+KA ++GTKLKDFVAT++S
Sbjct: 423 SAMVPGGIRMGTPALTSRGFLEEDFVKVADFFDAAVKIAVKVKAETQGTKLKDFVATLES 482

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           +   +SEI+KLR  VE+YAKQFPTIGFEKETMKY++
Sbjct: 483 SAPIKSEIAKLRHDVEEYAKQFPTIGFEKETMKYKN 518

BLAST of CmaCh12G001830 vs. ExPASy Swiss-Prot
Match: P34899 (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 883.6 bits (2282), Expect = 1.1e-255
Identity = 438/514 (85.21%), Postives = 476/514 (92.61%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALR+L+  V KS+ PL +   L++ SSL   A  +++  R TW KQLN+PL VIDPE
Sbjct: 3   MAMALRKLSSSVNKSSRPLFSASSLYYKSSLPDEAVYDKENPRVTWPKQLNSPLEVIDPE 62

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKG ELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 63  IADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMA 122

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ETLCQKRALEAFRLDPAKWGVNVQ LSGSP+NFQ YTALLKPH+RIMALDLPHGGHLSHG
Sbjct: 123 ETLCQKRALEAFRLDPAKWGVNVQPLSGSPSNFQVYTALLKPHDRIMALDLPHGGHLSHG 182

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFET+PYRLDESTGYIDYDQLEKSA+LFRPKLIVAGASAYARLYDYA
Sbjct: 183 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYA 242

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIR++CDKQKAV+LADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 243 RIRKVCDKQKAVLLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 302

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           +KE+NK+G+EV YDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEY+AYQEQVLS
Sbjct: 303 LKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQVLS 362

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NSSKFA++L EKGY+LVSGGT+NHLVLVNLKNKGIDGSRVEKVLE VHIAANKNTVPGDV
Sbjct: 363 NSSKFAKALSEKGYDLVSGGTENHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDV 422

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGF+E DFVKVAEYFD AV LALK+KA S+GTKLKDFV  +Q+
Sbjct: 423 SAMVPGGIRMGTPALTSRGFVEEDFVKVAEYFDAAVSLALKVKAESKGTKLKDFVEALQT 482

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKY 515
           +   QSEISKL+  VE++AKQFPTIGFEK TMKY
Sbjct: 483 SSYVQSEISKLKHDVEEFAKQFPTIGFEKATMKY 516

BLAST of CmaCh12G001830 vs. ExPASy Swiss-Prot
Match: Q9SZJ5 (Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM1 PE=1 SV=1)

HSP 1 Score: 874.8 bits (2259), Expect = 4.9e-253
Identity = 433/516 (83.91%), Postives = 477/516 (92.44%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALRRL+  + K   PL+      +MSSL   A +E++++R TW KQLNAPL  +DPE
Sbjct: 3   MAMALRRLSSSIDKPIRPLIRSTSC-YMSSLPSEAVDEKERSRVTWPKQLNAPLEEVDPE 62

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIE EKARQWKG ELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 63  IADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMA 122

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ETLCQKRALEAFRLDP KWGVNVQ LSGSPANF  YTALLKPHERIMALDLPHGGHLSHG
Sbjct: 123 ETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHG 182

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFET+PYRLDESTGYIDYDQ+EKSA+LFRPKLIVAGASAYARLYDYA
Sbjct: 183 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYA 242

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIR++C+KQKAVMLADMAHISGLVAA VIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 243 RIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 302

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKEINK+G+EVLYD+EDKINQAVFPGLQGGPHNHTITGLAVALKQATT EYKAYQEQVLS
Sbjct: 303 VKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVLS 362

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NS+KFAQ+L+E+GYELVSGGTDNHLVLVNLK KGIDGSRVEKVLE+VHIA+NKNTVPGDV
Sbjct: 363 NSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDV 422

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGF+E DF KVAEYFD+AV +ALK+K+ ++GTKLKDFV+ M+S
Sbjct: 423 SAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMES 482

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           +   QSEI+KLR +VE++AKQFPTIGFEKETMKY++
Sbjct: 483 SSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN 517

BLAST of CmaCh12G001830 vs. ExPASy Swiss-Prot
Match: P49357 (Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei OX=4226 PE=2 SV=1)

HSP 1 Score: 868.2 bits (2242), Expect = 4.6e-251
Identity = 432/516 (83.72%), Postives = 472/516 (91.47%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MA+ALRRL+    K    L NG  L+ MSSL   A  E+++   TW KQLNAPL V+DPE
Sbjct: 3   MALALRRLSSSADKPLQRLFNGGHLYSMSSLPSEAVYEKERPGVTWPKQLNAPLEVVDPE 62

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKG ELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 63  IADIIELEKARQWKGLELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMA 122

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ETLCQKRALEAFRLDPAKWGVNVQ LSGSPANF  YTALLK H+RIMALDLPHGGHLSHG
Sbjct: 123 ETLCQKRALEAFRLDPAKWGVNVQPLSGSPANFHVYTALLKAHDRIMALDLPHGGHLSHG 182

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFET+PYRL+ESTGYIDYDQLEKSA+LFRPKLIVAGASAYARLYDYA
Sbjct: 183 YQTDTKKISAVSIFFETMPYRLNESTGYIDYDQLEKSATLFRPKLIVAGASAYARLYDYA 242

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIR++CDKQKA+MLADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 243 RIRKVCDKQKAIMLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 302

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           +KE+NK+G+EV YDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATT EYKAYQEQV+S
Sbjct: 303 LKEVNKQGKEVFYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTAEYKAYQEQVMS 362

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NS+KFA++L++ GYELVSGGT+NHLVLVNLKNKGIDGS+VEKVLE+VHIAANKNTVPGDV
Sbjct: 363 NSAKFAETLVKSGYELVSGGTENHLVLVNLKNKGIDGSKVEKVLEAVHIAANKNTVPGDV 422

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGF+E DF KVA +FD AVKLA+KIK  ++GTKLKDFV  M+S
Sbjct: 423 SAMVPGGIRMGTPALTSRGFVEEDFAKVAYFFDLAVKLAVKIKGEAKGTKLKDFVTAMES 482

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           +   QSEISKLR  VE+YAKQFPTIGFEKETMKY++
Sbjct: 483 SA-IQSEISKLRHDVEEYAKQFPTIGFEKETMKYKN 517

BLAST of CmaCh12G001830 vs. ExPASy TrEMBL
Match: A0A6J1KI05 (Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495957 PE=3 SV=1)

HSP 1 Score: 1021.5 bits (2640), Expect = 1.2e-294
Identity = 516/516 (100.00%), Postives = 516/516 (100.00%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE
Sbjct: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD
Sbjct: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 516

BLAST of CmaCh12G001830 vs. ExPASy TrEMBL
Match: A0A6J1GHA4 (Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454162 PE=3 SV=1)

HSP 1 Score: 1015.4 bits (2624), Expect = 8.5e-293
Identity = 512/516 (99.22%), Postives = 514/516 (99.61%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALRR AVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPL VIDPE
Sbjct: 1   MAMALRRFAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRQICDKQKAVMLADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD
Sbjct: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 516

BLAST of CmaCh12G001830 vs. ExPASy TrEMBL
Match: A0A1S3BBK2 (Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103488144 PE=3 SV=1)

HSP 1 Score: 970.3 bits (2507), Expect = 3.2e-279
Identity = 487/516 (94.38%), Postives = 502/516 (97.29%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALRR+AVGVT   +PL+N RFLH MSSLS AAAEER+KARATWI+QLNAPL VIDPE
Sbjct: 1   MAMALRRIAVGVTNPMLPLLNARFLHRMSSLSEAAAEEREKARATWIQQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKG ELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           E+LCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ESLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFET+PYRLDESTGYIDYDQLE+SASLFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIR++CDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           N SKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NCSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADFVKVAE+FDE VKLALKIK  SEGTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEFFDETVKLALKIKGGSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           NKDFQ+EISKLR +VE+YAKQFPTIGFEKETM+YRD
Sbjct: 481 NKDFQAEISKLRHQVEEYAKQFPTIGFEKETMRYRD 516

BLAST of CmaCh12G001830 vs. ExPASy TrEMBL
Match: A0A5A7VGP9 (Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G004050 PE=3 SV=1)

HSP 1 Score: 965.7 bits (2495), Expect = 7.8e-278
Identity = 487/517 (94.20%), Postives = 502/517 (97.10%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALRR+AVGVT   +PL+N RFLH MSSLS AAAEER+KARATWI+QLNAPL VIDPE
Sbjct: 1   MAMALRRIAVGVTNPMLPLLNARFLHRMSSLSEAAAEEREKARATWIQQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKG ELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           E+LCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ESLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFET+PYRLDESTGYIDYDQLE+SASLFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIR++CDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           N SKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NCSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEG-TKLKDFVATMQ 480
           SAMVPGGIRMGTPALTSRGFLEADFVKVAE+FDE VKLALKIK  SEG TKLKDFVATMQ
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEFFDETVKLALKIKGGSEGCTKLKDFVATMQ 480

Query: 481 SNKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           SNKDFQ+EISKLR +VE+YAKQFPTIGFEKETM+YRD
Sbjct: 481 SNKDFQAEISKLRHQVEEYAKQFPTIGFEKETMRYRD 517

BLAST of CmaCh12G001830 vs. ExPASy TrEMBL
Match: A0A6J1BS86 (Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111004911 PE=3 SV=1)

HSP 1 Score: 962.6 bits (2487), Expect = 6.6e-277
Identity = 481/514 (93.58%), Postives = 499/514 (97.08%), Query Frame = 0

Query: 3   MALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPEIA 62
           MALRRL V V K   PLVNGRFLHHMSSLSGAA E+R+KARATWIKQLNAPL VIDPEIA
Sbjct: 1   MALRRLLVAVRKPTDPLVNGRFLHHMSSLSGAATEDREKARATWIKQLNAPLEVIDPEIA 60

Query: 63  DIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAET 122
           DIIELEKARQWKG ELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAE+
Sbjct: 61  DIIELEKARQWKGLELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMAES 120

Query: 123 LCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQ 182
           LCQKRALEAF LDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQ
Sbjct: 121 LCQKRALEAFSLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHGYQ 180

Query: 183 TDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYARI 242
           TDTKKISAVSIFFET+PYRLDESTGYIDYDQLE++A+LFRPKLIVAGASAYARLYDYARI
Sbjct: 181 TDTKKISAVSIFFETMPYRLDESTGYIDYDQLERNAALFRPKLIVAGASAYARLYDYARI 240

Query: 243 RQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVK 302
           R++CDKQKA+MLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVK
Sbjct: 241 RKVCDKQKAIMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKGVK 300

Query: 303 EINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLSNS 362
           EINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQVLSN 
Sbjct: 301 EINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLSNC 360

Query: 363 SKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSA 422
           S+FAQSLIEKGYELVS GTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSA
Sbjct: 361 SRFAQSLIEKGYELVSAGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDVSA 420

Query: 423 MVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQSNK 482
           MVPGGIRMGTPALTSRGFLEADF KVAE+FDEAVKLALKIKA+S+GTKLKDFVATMQSNK
Sbjct: 421 MVPGGIRMGTPALTSRGFLEADFAKVAEFFDEAVKLALKIKADSQGTKLKDFVATMQSNK 480

Query: 483 DFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           DFQSEISKLR +VE+YAKQFPTIGFEKETM+YR+
Sbjct: 481 DFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRN 514

BLAST of CmaCh12G001830 vs. NCBI nr
Match: XP_023001927.1 (serine hydroxymethyltransferase 2, mitochondrial [Cucurbita maxima] >XP_023001928.1 serine hydroxymethyltransferase 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 1021.5 bits (2640), Expect = 2.5e-294
Identity = 516/516 (100.00%), Postives = 516/516 (100.00%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE
Sbjct: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD
Sbjct: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 516

BLAST of CmaCh12G001830 vs. NCBI nr
Match: XP_022951277.1 (serine hydroxymethyltransferase 2, mitochondrial [Cucurbita moschata] >XP_022951278.1 serine hydroxymethyltransferase 2, mitochondrial [Cucurbita moschata])

HSP 1 Score: 1015.4 bits (2624), Expect = 1.8e-292
Identity = 512/516 (99.22%), Postives = 514/516 (99.61%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALRR AVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPL VIDPE
Sbjct: 1   MAMALRRFAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRQICDKQKAVMLADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD
Sbjct: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 516

BLAST of CmaCh12G001830 vs. NCBI nr
Match: XP_023538371.1 (serine hydroxymethyltransferase 2, mitochondrial isoform X2 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1000.7 bits (2586), Expect = 4.5e-288
Identity = 508/517 (98.26%), Postives = 512/517 (99.03%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHM-SSLSGAAAEERDKARATWIKQLNAPLGVIDP 60
           MAMALRR AVGVTKSNVPLVNGRFL  + SSLSGAAAEERDKARATWIKQLNAPL VIDP
Sbjct: 1   MAMALRRFAVGVTKSNVPLVNGRFLXMIFSSLSGAAAEERDKARATWIKQLNAPLEVIDP 60

Query: 61  EIADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM 120
           EIADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EIADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 AETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH 180
           AETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDY 240
           GYQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 300
           ARIRQICDKQKAVMLADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRQICDKQKAVMLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 360
           GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL
Sbjct: 301 GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 360

Query: 361 SNSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGD 420
           SNSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGD
Sbjct: 361 SNSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGD 420

Query: 421 VSAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQ 480
           VSAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQ
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQ 480

Query: 481 SNKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           +NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD
Sbjct: 481 ANKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517

BLAST of CmaCh12G001830 vs. NCBI nr
Match: XP_004148411.1 (serine hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KAE8652150.1 hypothetical protein Csa_022369 [Cucumis sativus])

HSP 1 Score: 976.5 bits (2523), Expect = 9.1e-281
Identity = 491/516 (95.16%), Postives = 504/516 (97.67%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALRR+AVGVT   +PL+N RFLH MSSLSGAAAEER+KARATWI+QLNAPL VIDPE
Sbjct: 1   MAMALRRIAVGVTNPILPLLNARFLHRMSSLSGAAAEEREKARATWIQQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           E+LCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ESLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFET+PYRLDESTGYIDYDQLE+SASLFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQLERSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIR++CDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRKVCDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTI GLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTIAGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           N SKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NCSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADFVKVAE FDE VKLALKIKA SEGTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAELFDETVKLALKIKAGSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           NKDFQSEISKLR +VE+YAKQFPTIGFEKETM+YRD
Sbjct: 481 NKDFQSEISKLRHQVEEYAKQFPTIGFEKETMRYRD 516

BLAST of CmaCh12G001830 vs. NCBI nr
Match: KAG6585322.1 (Serine hydroxymethyltransferase 2, mitochondrial, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 974.2 bits (2517), Expect = 4.5e-280
Identity = 492/497 (98.99%), Postives = 494/497 (99.40%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALRR AVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPL VIDPE
Sbjct: 1   MAMALRRFAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLEVIDPE 60

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ETLCQKRALEAF LDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG
Sbjct: 121 ETLCQKRALEAFHLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA
Sbjct: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIRQICDKQKAVMLADMAHISGLVAAGVIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 300

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS
Sbjct: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV
Sbjct: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS
Sbjct: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480

Query: 481 NKDFQSEISKLRLKVEQ 498
           NKDFQSEISKLRLKVEQ
Sbjct: 481 NKDFQSEISKLRLKVEQ 497

BLAST of CmaCh12G001830 vs. TAIR 10
Match: AT5G26780.1 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 890.2 bits (2299), Expect = 8.0e-259
Identity = 445/517 (86.07%), Postives = 482/517 (93.23%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKS-NVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDP 60
           MA+ALRRL+  V K  ++   NG  L  MSSLS AA  E +K+R++WIKQLNA L  IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  EIADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM 120
           E+ADIIELEKARQWKGFELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 AETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH 180
           AETLCQKRALEAF+LDP+KWGVNVQSLSGSPANFQ YTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDY 240
           GYQTDTKKISAVSIFFET+PYRLDE+TGYIDYDQLEKSA LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 300
           ARIR++C+KQKAVMLADMAHISGLVAAGVIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 360
           G+KEINK+G+EV+YDYED+INQAVFPGLQGGPHNHTITGLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 SNSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGD 420
            N SKFA++L+ KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKVLE VHIAANKNTVPGD
Sbjct: 361 RNCSKFAETLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGD 420

Query: 421 VSAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQ 480
           VSAMVPGGIRMGTPALTSRGF+E DF KVAEYFD AVK+ALKIKA S+GTKLKDFVATMQ
Sbjct: 421 VSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIKAESQGTKLKDFVATMQ 480

Query: 481 SNKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           SN+  QSE+SKLR  VE+YAKQFPTIGFEKETM+Y++
Sbjct: 481 SNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYKE 517

BLAST of CmaCh12G001830 vs. TAIR 10
Match: AT5G26780.3 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 880.9 bits (2275), Expect = 4.8e-256
Identity = 446/533 (83.68%), Postives = 482/533 (90.43%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKS-NVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDP 60
           MA+ALRRL+  V K  ++   NG  L  MSSLS AA  E +K+R++WIKQLNA L  IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  EIADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM 120
           E+ADIIELEKARQWKGFELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 AETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH 180
           AETLCQKRALEAF+LDP+KWGVNVQSLSGSPANFQ YTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDY 240
           GYQTDTKKISAVSIFFET+PYRLDE+TGYIDYDQLEKSA LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 300
           ARIR++C+KQKAVMLADMAHISGLVAAGVIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 360
           G+KEINK+G+EV+YDYED+INQAVFPGLQGGPHNHTITGLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 SNSSKFA----------------QSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKV 420
            N SKFA                Q+L+ KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKV
Sbjct: 361 RNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKV 420

Query: 421 LESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIK 480
           LE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+E DF KVAEYFD AVK+ALKIK
Sbjct: 421 LELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIK 480

Query: 481 ANSEGTKLKDFVATMQSNKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           A S+GTKLKDFVATMQSN+  QSE+SKLR  VE+YAKQFPTIGFEKETM+Y++
Sbjct: 481 AESQGTKLKDFVATMQSNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYKE 533

BLAST of CmaCh12G001830 vs. TAIR 10
Match: AT5G26780.2 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 880.9 bits (2275), Expect = 4.8e-256
Identity = 446/533 (83.68%), Postives = 482/533 (90.43%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKS-NVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDP 60
           MA+ALRRL+  V K  ++   NG  L  MSSLS AA  E +K+R++WIKQLNA L  IDP
Sbjct: 1   MALALRRLSSSVKKPISLLSSNGGSLRFMSSLSTAAMAESEKSRSSWIKQLNASLDEIDP 60

Query: 61  EIADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDM 120
           E+ADIIELEKARQWKGFELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDM
Sbjct: 61  EVADIIELEKARQWKGFELIPSENFTSLSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDM 120

Query: 121 AETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSH 180
           AETLCQKRALEAF+LDP+KWGVNVQSLSGSPANFQ YTALLKPHERIMALDLPHGGHLSH
Sbjct: 121 AETLCQKRALEAFQLDPSKWGVNVQSLSGSPANFQVYTALLKPHERIMALDLPHGGHLSH 180

Query: 181 GYQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDY 240
           GYQTDTKKISAVSIFFET+PYRLDE+TGYIDYDQLEKSA LFRPKLIVAGASAYARLYDY
Sbjct: 181 GYQTDTKKISAVSIFFETMPYRLDENTGYIDYDQLEKSAVLFRPKLIVAGASAYARLYDY 240

Query: 241 ARIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRK 300
           ARIR++C+KQKAVMLADMAHISGLVAAGVIPSPFEYAD+VTTTTHKSLRGPRGAMIFFRK
Sbjct: 241 ARIRKVCNKQKAVMLADMAHISGLVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRK 300

Query: 301 GVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVL 360
           G+KEINK+G+EV+YDYED+INQAVFPGLQGGPHNHTITGLAVALKQA TPEYKAYQ+QVL
Sbjct: 301 GLKEINKQGKEVMYDYEDRINQAVFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVL 360

Query: 361 SNSSKFA----------------QSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKV 420
            N SKFA                Q+L+ KGY+LVSGGTDNHLVLVNLKNKGIDGSRVEKV
Sbjct: 361 RNCSKFAELDIRPTVIISYGLSMQTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKV 420

Query: 421 LESVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIK 480
           LE VHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGF+E DF KVAEYFD AVK+ALKIK
Sbjct: 421 LELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIEEDFAKVAEYFDLAVKIALKIK 480

Query: 481 ANSEGTKLKDFVATMQSNKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           A S+GTKLKDFVATMQSN+  QSE+SKLR  VE+YAKQFPTIGFEKETM+Y++
Sbjct: 481 AESQGTKLKDFVATMQSNEKLQSEMSKLREMVEEYAKQFPTIGFEKETMRYKE 533

BLAST of CmaCh12G001830 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 874.8 bits (2259), Expect = 3.5e-254
Identity = 433/516 (83.91%), Postives = 477/516 (92.44%), Query Frame = 0

Query: 1   MAMALRRLAVGVTKSNVPLVNGRFLHHMSSLSGAAAEERDKARATWIKQLNAPLGVIDPE 60
           MAMALRRL+  + K   PL+      +MSSL   A +E++++R TW KQLNAPL  +DPE
Sbjct: 3   MAMALRRLSSSIDKPIRPLIRSTSC-YMSSLPSEAVDEKERSRVTWPKQLNAPLEEVDPE 62

Query: 61  IADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGGNEYIDMA 120
           IADIIE EKARQWKG ELIPSENFTS SVM+AVGSVMTNKYSEGYPGARYYGGNEYIDMA
Sbjct: 63  IADIIEHEKARQWKGLELIPSENFTSVSVMQAVGSVMTNKYSEGYPGARYYGGNEYIDMA 122

Query: 121 ETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPHGGHLSHG 180
           ETLCQKRALEAFRLDP KWGVNVQ LSGSPANF  YTALLKPHERIMALDLPHGGHLSHG
Sbjct: 123 ETLCQKRALEAFRLDPEKWGVNVQPLSGSPANFHVYTALLKPHERIMALDLPHGGHLSHG 182

Query: 181 YQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAYARLYDYA 240
           YQTDTKKISAVSIFFET+PYRLDESTGYIDYDQ+EKSA+LFRPKLIVAGASAYARLYDYA
Sbjct: 183 YQTDTKKISAVSIFFETMPYRLDESTGYIDYDQMEKSATLFRPKLIVAGASAYARLYDYA 242

Query: 241 RIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGAMIFFRKG 300
           RIR++C+KQKAVMLADMAHISGLVAA VIPSPF+YAD+VTTTTHKSLRGPRGAMIFFRKG
Sbjct: 243 RIRKVCNKQKAVMLADMAHISGLVAANVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKG 302

Query: 301 VKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKAYQEQVLS 360
           VKEINK+G+EVLYD+EDKINQAVFPGLQGGPHNHTITGLAVALKQATT EYKAYQEQVLS
Sbjct: 303 VKEINKQGKEVLYDFEDKINQAVFPGLQGGPHNHTITGLAVALKQATTSEYKAYQEQVLS 362

Query: 361 NSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANKNTVPGDV 420
           NS+KFAQ+L+E+GYELVSGGTDNHLVLVNLK KGIDGSRVEKVLE+VHIA+NKNTVPGDV
Sbjct: 363 NSAKFAQTLMERGYELVSGGTDNHLVLVNLKPKGIDGSRVEKVLEAVHIASNKNTVPGDV 422

Query: 421 SAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKDFVATMQS 480
           SAMVPGGIRMGTPALTSRGF+E DF KVAEYFD+AV +ALK+K+ ++GTKLKDFV+ M+S
Sbjct: 423 SAMVPGGIRMGTPALTSRGFVEEDFAKVAEYFDKAVTIALKVKSEAQGTKLKDFVSAMES 482

Query: 481 NKDFQSEISKLRLKVEQYAKQFPTIGFEKETMKYRD 517
           +   QSEI+KLR +VE++AKQFPTIGFEKETMKY++
Sbjct: 483 SSTIQSEIAKLRHEVEEFAKQFPTIGFEKETMKYKN 517

BLAST of CmaCh12G001830 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 566.2 bits (1458), Expect = 2.6e-161
Identity = 280/454 (61.67%), Postives = 345/454 (75.99%), Query Frame = 0

Query: 54  LGVIDPEIADIIELEKARQWKGFELIPSENFTSSSVMEAVGSVMTNKYSEGYPGARYYGG 113
           LG +DPE+  II  EK RQ++  ELI SENFTS +VMEAVGS +TNKYSEG PG RYYGG
Sbjct: 83  LGEVDPEVRTIITKEKDRQFRSLELIASENFTSRAVMEAVGSCLTNKYSEGLPGKRYYGG 142

Query: 114 NEYIDMAETLCQKRALEAFRLDPAKWGVNVQSLSGSPANFQAYTALLKPHERIMALDLPH 173
           NEYID  ETLCQ RAL AFRLD  KWGVNVQ LSGSPANF  YTA+L PH+RIM LDLPH
Sbjct: 143 NEYIDQLETLCQNRALAAFRLDSTKWGVNVQPLSGSPANFAVYTAILSPHDRIMGLDLPH 202

Query: 174 GGHLSHGYQTDTKKISAVSIFFETVPYRLDESTGYIDYDQLEKSASLFRPKLIVAGASAY 233
           GGHLSHG+ T  +++S  SI+FE++PYRLDESTG +DYD LEK+A+LFRPKLI+AGASAY
Sbjct: 203 GGHLSHGFMTAKRRVSGTSIYFESMPYRLDESTGIVDYDMLEKTATLFRPKLIIAGASAY 262

Query: 234 ARLYDYARIRQICDKQKAVMLADMAHISGLVAAGVIPSPFEYADIVTTTTHKSLRGPRGA 293
           +R +DY R+R+I D   A ++ DMAHISGLVAA V+  PFEY DIVTTTTHKSLRGPRG 
Sbjct: 263 SRDFDYPRMRKIADSVGAFLMMDMAHISGLVAASVVADPFEYCDIVTTTTHKSLRGPRGG 322

Query: 294 MIFFRKGVKEINKKGQEVLYDYEDKINQAVFPGLQGGPHNHTITGLAVALKQATTPEYKA 353
           MIFFRK    IN        D E  +N AVFPGLQGGPHNHTI GLAV LK A +PE+KA
Sbjct: 323 MIFFRK--DPING------VDLESAVNNAVFPGLQGGPHNHTIGGLAVCLKHAQSPEFKA 382

Query: 354 YQEQVLSNSSKFAQSLIEKGYELVSGGTDNHLVLVNLKNKGIDGSRVEKVLESVHIAANK 413
           YQ++V+SN    A  L+E G++LVSGG+DNHLVLV+L+  G+DG+RVEK+L+   I  NK
Sbjct: 383 YQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGARVEKILDMASITLNK 442

Query: 414 NTVPGDVSAMVPGGIRMGTPALTSRGFLEADFVKVAEYFDEAVKLALKIKANSEGTKLKD 473
           N+VPGD SA+VPGGIR+G+PA+T+RG  E DFV VA++  E V++ ++ K  + G+KL+D
Sbjct: 443 NSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEITMEAKKAAPGSKLQD 502

Query: 474 FVATMQSNK-DFQSEISKLRLKVEQYAKQFPTIG 507
           F   + S +   +  +  L+ +VE +  +FP  G
Sbjct: 503 FNKFVTSPEFPLKERVKSLKERVETFTSRFPIPG 528

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q94C741.1e-25786.07Serine hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
P504331.2e-25684.88Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2... [more]
P348991.1e-25585.21Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=... [more]
Q9SZJ54.9e-25383.91Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
P493574.6e-25183.72Serine hydroxymethyltransferase 1, mitochondrial OS=Flaveria pringlei OX=4226 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1KI051.2e-294100.00Serine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495957 PE=3... [more]
A0A6J1GHA48.5e-29399.22Serine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454162 PE... [more]
A0A1S3BBK23.2e-27994.38Serine hydroxymethyltransferase OS=Cucumis melo OX=3656 GN=LOC103488144 PE=3 SV=... [more]
A0A5A7VGP97.8e-27894.20Serine hydroxymethyltransferase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
A0A6J1BS866.6e-27793.58Serine hydroxymethyltransferase OS=Momordica charantia OX=3673 GN=LOC111004911 P... [more]
Match NameE-valueIdentityDescription
XP_023001927.12.5e-294100.00serine hydroxymethyltransferase 2, mitochondrial [Cucurbita maxima] >XP_02300192... [more]
XP_022951277.11.8e-29299.22serine hydroxymethyltransferase 2, mitochondrial [Cucurbita moschata] >XP_022951... [more]
XP_023538371.14.5e-28898.26serine hydroxymethyltransferase 2, mitochondrial isoform X2 [Cucurbita pepo subs... [more]
XP_004148411.19.1e-28195.16serine hydroxymethyltransferase 2, mitochondrial [Cucumis sativus] >KAE8652150.1... [more]
KAG6585322.14.5e-28098.99Serine hydroxymethyltransferase 2, mitochondrial, partial [Cucurbita argyrosperm... [more]
Match NameE-valueIdentityDescription
AT5G26780.18.0e-25986.07serine hydroxymethyltransferase 2 [more]
AT5G26780.34.8e-25683.68serine hydroxymethyltransferase 2 [more]
AT5G26780.24.8e-25683.68serine hydroxymethyltransferase 2 [more]
AT4G37930.13.5e-25483.91serine transhydroxymethyltransferase 1 [more]
AT4G32520.12.6e-16161.67serine hydroxymethyltransferase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 481..501
NoneNo IPR availablePANTHERPTHR11680:SF46SERINE HYDROXYMETHYLTRANSFERASE, MITOCHONDRIALcoord: 4..515
IPR001085Serine hydroxymethyltransferasePIRSFPIRSF000412SHMTcoord: 35..511
e-value: 8.5E-169
score: 559.5
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 4..515
IPR001085Serine hydroxymethyltransferaseHAMAPMF_00051SHMTcoord: 53..506
score: 42.474243
IPR001085Serine hydroxymethyltransferaseCDDcd00378SHMTcoord: 58..478
e-value: 0.0
score: 635.782
IPR015421Pyridoxal phosphate-dependent transferase, major domainGENE3D3.40.640.10coord: 83..356
e-value: 5.7E-215
score: 715.7
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 58..503
e-value: 5.7E-215
score: 715.7
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 57..451
e-value: 2.4E-212
score: 704.7
IPR019798Serine hydroxymethyltransferase, pyridoxal phosphate binding sitePROSITEPS00096SHMTcoord: 277..293
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 50..506

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G001830.1CmaCh12G001830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046655 folic acid metabolic process
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0006565 L-serine catabolic process
biological_process GO:0032259 methylation
biological_process GO:0035999 tetrahydrofolate interconversion
cellular_component GO:0005737 cytoplasm
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0008168 methyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0070905 serine binding
molecular_function GO:0008270 zinc ion binding
molecular_function GO:0003824 catalytic activity