CmaCh12G001780 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G001780
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionSerine hydroxymethyltransferase
LocationCma_Chr12: 812991 .. 819155 (-)
RNA-Seq ExpressionCmaCh12G001780
SyntenyCmaCh12G001780
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDS
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGACTACTCTAGTGACTGGTTATGTTCAGAGTTCGCAGCCTCTGCCTGCCTTAAGTATTCTTCGAAATGTTGCAGGCTGGAGATTACCCTACAAATTTTACACTAGGAATAGTGTTGAATGCTTGTTCTTCCTTGCAATCTATCGAATTGAGGAAGCAAGTTCGGTTAGGAACCACGACTCTACATAATGGTATGATATTATCCATTTTGAACACAAACTCTCGTGGCTCTACTTTTGGTTTCTCCAAAAAGTCTCATACTAGTGGAGATGTATTTCTTACTTATAAATCCATGATCATCTCCTTAATTAGTTGATGTGAGACTTCCCACCTACAATCCTCAACAATCCTCACCTCGAACAAAGTATACCACAGAGCCTCCCCTGAGGCCTATGAAACCCTCAAATAGCCTCTCTTTAATTGAGGTTCGACTTCTTTCTCTGGAGCCCTCGAACAAAGTACATAGGGTGTACTTTGTACGACACTTTAGTTACTTTTTTACTACACCTTCAAGGCTCACAACTTCTTTATTGGACATTAGTATTTTATTGGCATGACTAAGTTAAGAACATGGCTTTGATACCATGTTAGAAACCACGACTTTCTACAATTGTATGATATTGTTCACTTTGAAGATGAACTCTCAAAAGGCCTCATATCAATGAAGATGTATTCCTTACTTATAAACTCATGTTCATCCCCTTTATTAGCCGACGTGGGACTTCCTTCCAACAATGGTACTAGTATTGGCAACTCTCTTTCTAGCTTCTATTCTAAATTCGGGTGCTTGGAATTTTCTGTAAGGGCCTTTCATAAAATTAAGGAAAAGCACGTCATTTCATGGACTTCAGGTCAAGCAGTTTGGAGTCCTAAGTTTTTTTTTTTTGTCGAGATGATCTCAGAAGGTGTTGAACCGAATGAATATACTCTCACAAGTGCCTTGAGCACTTGTTGCGTGATGCTAACATTGGATCTAGGAGCACAGATTCACTCACTAAGCATTGAGCTTGAGCTCATAAAGATATATTTGAGAGATATTGGAGTTTGATGTCTAGATCTGGTAAAGATATATTTGGGAGATATTTCGATGTGATAGATATATAGTAGATATTTGGTAAAGATGTGATAGAGATATATTTGTTAGATATCTATGGTTTAAATCTCTAGAGGCTATATTTAGTAAATTGAAACCGATTTGTTGTATTTTCTGCTCTCTCCAGTTATTTGTGTTCATCTTGATGGAGTGTGTGCTTTGTTGATCGATCCTAACAATTGGTATCAGAGCTAACAACAATTGGTATCGATCCTAACAACTGGTACAAGTAATGAATTGGTATCGATCCTAACAATTGGTATCAGAGCTAACAACAATTGGCATCTCTTCTTGTACAAGTAATGAATAAGCTTGCCTCACATTAGGTAATGGGACTGAGTTCCTATCTTTGCAACCATTCTTTACTTCTTGTGGTATTGAATTTCAGCGCACTTGTGTCTATACTCCACAACAAAATGGAGTCGTAGAACGTAAGCATCGCCATATCCTAAATGTAGCTAGGTCTCTTTTTTTTCAGTCACAGGTTCCACTTAATTTTTTGGGGAGAGTGCGTTTTAACGACTGTTTATCTTATAAATAGAACGCCATCACCAGTGTTATCTAACAAGACACCCTTTGAAGCACTCTACAAACGATCACCTACATTTCATCATCTTAAAGTTTTTGGTTGTAAATGTTATGCAACTATAGTACATCCTAAGCAAAAAATTGAACCTCGGGCAATTCCTTGTGTTTTCGTAGGATATCCTTGTGGTCATAAAGGTAACAAATTGTATGACATGCAATCTCACAAATTCTTTATCAGTCGTGATGTTAAATTTTGTGAAGATGATTTTCCTTTTTCATCAGCTTCACAAACTTCGACATTAGCTCCTTCGACTCCTGTTGTTCCACTTCATGATCCATCCTACTTAAGCACCCATCCTCCACCTTCCATTCCTCCTTCACCTCCTATCCCTTCACCTACTACTTCGTCGTCTCCTCCACCTTCTCCAGATTTGTCCACTGATTCCAATCCTATCCCGCCTGATACATCAGCTCCACTCCGACGTTCTATTCGCACTAAACAGCCTCCAGCTTGGCATAAGGATTATGAGATGTCTTCTGGAGCCAATCATTTAACCTCTAGCTCAAGTCCCGGCACTGGCACCAGGTATCCCCTTCATCATTACCTTTCATTCTCTCGTTTTTCTCCTACTCAACGTGCTTTTCTAGCTCTTATTACATCCCAGACAGAACCTAAAACCTATGACGAGGCAGTTGGCGACCCTTTATGGCAGCAGGCTATGAATGATGAAATTGCAGCTTTGGAACGTAATCATACATGGTCTCTCGTTCCTCTACCACCTGGTCATAAAGTTATTGGTTGTCGCTGGGTGTACAAAATTAAATACAACTCTGATGGTTCTGTTGAACGTTATAAAGCTCGACTTGTAGCAAAGGGATACACTCAGGTTGAAGGTATTGATTACACAGAAACATTTTCCCCTACAGCAAAACTTACTACACTTCGTTGCTTACTCACTGTTGCTGCTGCTCGAAAATGGTTCACCCATCAGTTGGATGTTCAAAATGCCTTTCTCCATGGTAATCTAGGCGAGGAAGTTTATATGTCTTTACCACCAGGTCTTCGCCGACAGGGGGAGAATACAGTATGTCGGCTCCATAAATCTCTTTATGGATTAAAACAGGCTTCTCGCAATTGGTTCTCCATATTTTCTACAACTATACAAAATGCAGGCTACACTCAGTCCAAAGCAGACTACTCTTTGTTTACCAAGAGTAAAGGTACTTCTTTCACTGCAGTTCTAATCTATGTTGATGATATTCTGTTGACAGGCAATGATCTCAAAGAAATGCAATATCTCAAGACTAGTTTACTCCAGAAATTTCTTATCAAAGATTTAGGAAATTTGAAATATTTTCTAGGCATTGAATTTTCTCGGTCTAGAAAAGGAATTTTTATGTCTCAAAGGAAGTATGCTCTAGACATCCTTCAAGACACAGGCCTTACACGAGCACGTCCAGACAAATTTCCTATGGAGCAAAATCTGAAACTTTCTTTAACTGAAGGAGAGAAATTGAACGATCCAAGTAAATACAGACGGTTGATTGGTAGATTAATATATTTGACTGTCACTAGGCCTGACATAGCTTATTCAGTTCGTATGCTTAGCCAATTTATGCATGAACCAAGAAAACCACATTGGGAGGCAGCTCTTCGAGTTCTGAGGTACATCAAAGGCACTCCTGGTCAAGGACTCCTACTGCCATCTGAAAACAATTTAAGATTACAAGCATATTGCGATTCTGACTGGGGTGGTTGTCGAACTTCCAGACGGTCTATTTCTGGATTCTGCATTTTCCTTGGGAATTCAATTATTTCTTGGAAGTCTAAAAAGCAGACTAATGTGTCCAGATCATCAGCAGAAGCCGAGTATCGAGCTATGGCAAATACTTGTTTAGAGTTAACTTGGTTAAGATACATTCTTCAAGACTTGAATGTTCCACTGTCCGAACCAGCATTATTATATTGTGATAATCAAGCAGCATTACATATACCAGCCAATCCAGTTTTTCACGAACGTACGAAACACATTGAAATAGATTGTCATATAATTCGAGAAAAGTTACAAGCTGGAATCATCAAACCATGTTATATTTCGACCAAAATGCAATTGGCAGATGTTTTTACTAAAGCTCTGGGAAGACAGCAATTTGACCTTTTGAAGGACAAGTTGGGTGTGATCGACATACACTCTCCAACTTGAGGGGAGTATTGAGAGGATATTTTGGAGATTTAGTTAAATATATTATTTTTTTCTATTTTTAGGTATTAGTTAATAGTTTGTATCTATTTAAACGTGGTAAACCTGAATGAAGATCATACACTTTTCCAGTTCTATTTTCTATTTCTATTTCTTAACAGTAATCATGATGGCTGATATGGCACACCTTGCTGGTTTAGTTGCAGCAGACGTTATTCCATCTCGTTTTGAATATGCTGATATTGTGACAACAACCACAAATAAGTCCTTAGGTGGACCACGAGGGGCTTTGATTTTCTATCGAAAGGGCGTTAAAGAGATCAACAAAAAAGGAGAGGAAGTAAGTGAGACGTAAAAATGTAGCTAAAAAAATAACCAAACTTTATTTGTCTTCTAATATTCGGGAGCCCTCGAACAAAGTACACCCTTTGTTCGACACTTTAGTCACTTTTGACTACACCTTCGAGGCTCACAATTCTTTGTTCGATATTTGAGGATTCTATTGACATAGCTAAGTTTAAGGCATGGCTCTAATATCATGTTAGGAATCACGACTCTCCACAATGGTATGATATTGTTCACTTTGAGCATAACCTCTCATGGATTTGCTTTAGGCTTCCCCAAAAGGCCTCGTACCAATGGAAATAGTATTCCTCACTTATAAACTCATGATCTTTCATTAAATTAACTAACATGGGACTCACTCCCAATAATCCTTGATAAGGTGCTCTATGACTACAAAGACAAAATCAATGGCACGGTCTTCCCTGGTTTTCAAGGTTCCCCACACAACCATAAAATTGCTGGTCCAGTGATTGCGCTAAAGCAGGTTGTTTATTTAATTCAAAACATCCTCGGTAGCAGAATTAAATTGTTTTCATTCATGTTATTTCTCTTCTTTACCATATTACAGTAGGCAACAACTCCGGAATACAAAGCCTACCAACAACAGGTTCTCAATAATTCTTCAAGATTTGCTCAGGTATACAGAATAGTGCATGTTTTTCTCACTGCTTTGAATTCTTTGCTTAAATCAGTGTAGTTTACTGTGTCAAATTCATTGTTTATGCAGTGTTTGATTGAGAAGGGGTATGAACTTGTATCTGGTGGAACTGATAATCATTTAGTCCTCGTGAATCTGAAAAATGAGGTAAAGGGTGAGTTTTGGTTCATCGTTATAACTATTATACTTCTCATCCACGTATTTTTCCTTGGACAGTGATCGAGATTACAATTTTATTAACGGTTTAACTAATAGTAAGAGTCTATTTAGAATAACTTTTCAAGTGTTAGAAAGACGAGTTTAAAAACCAAATAGATATAATTTCAAAAGTCTGGGGACTAAATTTGTAATTTAACCTATTTTCAACCTTACGATATATAAATGTTAATCTGGCAGGGTATTGATGGCTCGAGAGTGGAAAAGGTTTTGGATTGGGTGCATATTGCAGCTAACAAGAACACAGTTCCAGGGGACGTGTCCGCCATTTCTCCGAGTGGCATTCGTATGGGTATGACTTTGGAGTTCATGATATAAAATAATATGTGATGTAATATAACATATCAGATACTGAATAAGAGTATAGACTATGATAATACTATAAATTTTGTGTATATAGGAACCCCTGCTCTTACTTCCAGAGGCTTCCTCGAAGCAGATTTTGTGAAAGTAGCTGAATTTTATGATGAAGCTACGAAGCTAGCCTTGAAGATTAAGGCTGCTGAACCTAAAGGTTGGGTTCTCGATCATAACAATTATCATTGTTAGATGATGAAAGTTCCACGTCGGCTAATTTAGGAAATATTCATGGATTTATAATCAAAGAATACTCTCTCCATTGGTATGAGGCATTTTGGGGAAGCCCAAAGCAAAGCCACGAGAGCTTATGCATAAAGTGGATAATATCATACCATTGTCTCTCCATTGGTACGAGGCTTTTTTGAGAAATCCAAATCAAAGTCATAAGAGCTTATGTTTAAAGTGTATAATATCATATCATTGTAGAGAGTCGTGTTCATCTAACAATCATATCTCCTTTAAAATATGATGACCTCCCGTCTTGCATCGCCTCGCTTTGCATGGCCCACTGGCTGTTAGAATTTTTTGATATGCTCAATATTTGTTTTAAAATATTGTAAACACAGGTGCTAAGTTAAAGGATTTCGAGGCAACCATGCAATTAAATAAAGAATTCCAATCTGAAATTTCAAAGCTCCGACGTGAGGTTGAGGAGTTTTCCAAGCAATTTCCAACCGTAGGTTTTGAGAAAGAGACAATGAAATATAGACTGAAGAACAGAGTTTTAAGGCACATGCAAAGTTTCGCAGCCGAGTCGGGTTAG

mRNA sequence

ATGGACTACTCTAGTGACTGGTTATGTTCAGAGTTCGCAGCCTCTGCCTGCCTTAAGGCCTTTCATAAAATTAAGGAAAAGCACGTCATTTCATGGACTTCAGGTCAAGCAGTTTGGAGTCCTAAGTTTTTTTTTTTTGTCGAGATGATCTCAGAAGGTGTTGAACCGAATGAATATACTCTCACAAGTGCCTTGAGCACTTGTTGCTCCTTAGGTGGACCACGAGGGGCTTTGATTTTCTATCGAAAGGGCGTTAAAGAGATCAACAAAAAAGGAGAGGAAGCAACAACTCCGGAATACAAAGCCTACCAACAACAGGTTCTCAATAATTCTTCAAGATTTGCTCAGTGTTTGATTGAGAAGGGGTATGAACTTGTATCTGGTGGAACTGATAATCATTTAGTCCTCGTGAATCTGAAAAATGAGGTAAAGGCTAACAAGAACACAGTTCCAGGGGACGTGTCCGCCATTTCTCCGAGTGGCATTCGTATGGGAACCCCTGCTCTTACTTCCAGAGGCTTCCTCGAAGCAGATTTTGTGAAAGATTTCGAGGCAACCATGCAATTAAATAAAGAATTCCAATCTGAAATTTCAAAGCTCCGACGTGAGGTTGAGGAGTTTTCCAAGCAATTTCCAACCGTAGGTTTTGAGAAAGAGACAATGAAATATAGACTGAAGAACAGAGTTTTAAGGCACATGCAAAGTTTCGCAGCCGAGTCGGGTTAG

Coding sequence (CDS)

ATGGACTACTCTAGTGACTGGTTATGTTCAGAGTTCGCAGCCTCTGCCTGCCTTAAGGCCTTTCATAAAATTAAGGAAAAGCACGTCATTTCATGGACTTCAGGTCAAGCAGTTTGGAGTCCTAAGTTTTTTTTTTTTGTCGAGATGATCTCAGAAGGTGTTGAACCGAATGAATATACTCTCACAAGTGCCTTGAGCACTTGTTGCTCCTTAGGTGGACCACGAGGGGCTTTGATTTTCTATCGAAAGGGCGTTAAAGAGATCAACAAAAAAGGAGAGGAAGCAACAACTCCGGAATACAAAGCCTACCAACAACAGGTTCTCAATAATTCTTCAAGATTTGCTCAGTGTTTGATTGAGAAGGGGTATGAACTTGTATCTGGTGGAACTGATAATCATTTAGTCCTCGTGAATCTGAAAAATGAGGTAAAGGCTAACAAGAACACAGTTCCAGGGGACGTGTCCGCCATTTCTCCGAGTGGCATTCGTATGGGAACCCCTGCTCTTACTTCCAGAGGCTTCCTCGAAGCAGATTTTGTGAAAGATTTCGAGGCAACCATGCAATTAAATAAAGAATTCCAATCTGAAATTTCAAAGCTCCGACGTGAGGTTGAGGAGTTTTCCAAGCAATTTCCAACCGTAGGTTTTGAGAAAGAGACAATGAAATATAGACTGAAGAACAGAGTTTTAAGGCACATGCAAAGTTTCGCAGCCGAGTCGGGTTAG

Protein sequence

MDYSSDWLCSEFAASACLKAFHKIKEKHVISWTSGQAVWSPKFFFFVEMISEGVEPNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEEATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLKNEVKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADFVKDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKETMKYRLKNRVLRHMQSFAAESG
Homology
BLAST of CmaCh12G001780 vs. ExPASy Swiss-Prot
Match: P50433 (Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2 SV=1)

HSP 1 Score: 212.2 bits (539), Expect = 6.4e-54
Identity = 124/253 (49.01%), Postives = 153/253 (60.47%), Query Frame = 0

Query: 49  MISEGVEPNEYTLTSALSTCC--SLGGPRGALIFYRKGVKEINKKGEE------------ 108
           +++ GV P+ +     ++T    SL GPRGA+IFYRKGVKE+NK+G+E            
Sbjct: 265 LVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFYRKGVKEVNKQGKEVFYDYEDKINQA 324

Query: 109 -----------------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTD 168
                                  ATTPEY+AYQ+QVL+NSS+FAQ L EKGYELVSGGTD
Sbjct: 325 VFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQVLSNSSKFAQALGEKGYELVSGGTD 384

Query: 169 NHLVLVNLKNE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLE 225
           NHLVLVN+KN+               + ANKNTVPGDVSA+ P GIRMGTPALTSRGFLE
Sbjct: 385 NHLVLVNMKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLE 444

BLAST of CmaCh12G001780 vs. ExPASy Swiss-Prot
Match: Q94C74 (Serine hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM2 PE=1 SV=2)

HSP 1 Score: 206.5 bits (524), Expect = 3.5e-52
Identity = 123/253 (48.62%), Postives = 150/253 (59.29%), Query Frame = 0

Query: 49  MISEGVEPN--EYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE------------ 108
           +++ GV P+  EY      +T  SL GPRGA+IF+RKG+KEINK+G+E            
Sbjct: 264 LVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGLKEINKQGKEVMYDYEDRINQA 323

Query: 109 -----------------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTD 168
                                  A TPEYKAYQ QVL N S+FA+ L+ KGY+LVSGGTD
Sbjct: 324 VFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVLRNCSKFAETLLAKGYDLVSGGTD 383

Query: 169 NHLVLVNLKNE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLE 225
           NHLVLVNLKN+               + ANKNTVPGDVSA+ P GIRMGTPALTSRGF+E
Sbjct: 384 NHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIE 443

BLAST of CmaCh12G001780 vs. ExPASy Swiss-Prot
Match: P34899 (Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=1)

HSP 1 Score: 203.0 bits (515), Expect = 3.9e-51
Identity = 119/252 (47.22%), Postives = 149/252 (59.13%), Query Frame = 0

Query: 49  MISEGVEPNEYTLTSALSTCC--SLGGPRGALIFYRKGVKEINKKGEE------------ 108
           +++ GV P+ +     ++T    SL GPRGA+IF+RKG+KE+NK+G+E            
Sbjct: 265 LVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGLKEVNKQGKEVFYDYEDKINQA 324

Query: 109 -----------------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTD 168
                                  ATTPEY+AYQ+QVL+NSS+FA+ L EKGY+LVSGGT+
Sbjct: 325 VFPGLQGGPHNHTITGLAVALKQATTPEYRAYQEQVLSNSSKFAKALSEKGYDLVSGGTE 384

Query: 169 NHLVLVNLKNE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLE 224
           NHLVLVNLKN+               + ANKNTVPGDVSA+ P GIRMGTPALTSRGF+E
Sbjct: 385 NHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE 444

BLAST of CmaCh12G001780 vs. ExPASy Swiss-Prot
Match: Q9SZJ5 (Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702 GN=SHM1 PE=1 SV=1)

HSP 1 Score: 202.2 bits (513), Expect = 6.6e-51
Identity = 120/244 (49.18%), Postives = 145/244 (59.43%), Query Frame = 0

Query: 56  PNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE--------------------- 115
           P +Y      +T  SL GPRGA+IF+RKGVKEINK+G+E                     
Sbjct: 273 PFDYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGKEVLYDFEDKINQAVFPGLQGGP 332

Query: 116 --------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK 175
                         ATT EYKAYQ+QVL+NS++FAQ L+E+GYELVSGGTDNHLVLVNLK
Sbjct: 333 HNHTITGLAVALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLK 392

Query: 176 NE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADF------ 225
            +               + +NKNTVPGDVSA+ P GIRMGTPALTSRGF+E DF      
Sbjct: 393 PKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEY 452

BLAST of CmaCh12G001780 vs. ExPASy Swiss-Prot
Match: P49358 (Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei OX=4226 PE=2 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 1.1e-50
Identity = 119/253 (47.04%), Postives = 151/253 (59.68%), Query Frame = 0

Query: 49  MISEGVEPNEYTLTSALSTCC--SLGGPRGALIFYRKGVKEINKKGEE------------ 108
           +++ GV P+ +     ++T    SL GPRGA+IF+RKGVKE+NK+G+E            
Sbjct: 265 LVAAGVIPSPFDYADVVTTTTHKSLRGPRGAMIFFRKGVKEVNKQGKEVLYDYEDKINQA 324

Query: 109 -----------------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTD 168
                                  ATT EYKAYQ+QV++N ++FA+ L++ GYELVSGGT+
Sbjct: 325 VFPGLQGGPHNHTITGLAVALKQATTAEYKAYQEQVMSNCAKFAETLVKSGYELVSGGTE 384

Query: 169 NHLVLVNLKNE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLE 225
           NHLVLVNLKN+               + ANKNTVPGDVSA+ P GIRMGTPALTSRGF+E
Sbjct: 385 NHLVLVNLKNKGIDGSRVEKVLEAVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFVE 444

BLAST of CmaCh12G001780 vs. ExPASy TrEMBL
Match: A0A6J1KHZ9 (Glycine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495956 PE=3 SV=1)

HSP 1 Score: 297.4 bits (760), Expect = 5.6e-77
Identity = 178/297 (59.93%), Postives = 189/297 (63.64%), Query Frame = 0

Query: 23  KIKEKHVISWTSGQAVWSPKFFFFVEMISEGVEPNEYTLTSALSTCC--SLGGPRGALIF 82
           KI +KH       +A+          +++  V P+ +     ++T    SLGGPRGALIF
Sbjct: 245 KICDKH-------KAIMMADMAHLAGLVAADVIPSRFEYADIVTTTTNKSLGGPRGALIF 304

Query: 83  YRKGVKEINKKGEE-----------------------------------ATTPEYKAYQQ 142
           YRKGVKEINKKGEE                                   ATTPEYKAYQQ
Sbjct: 305 YRKGVKEINKKGEEVLYDYKDKINGTVFPGFQGSPHNHKIAGPVIALKQATTPEYKAYQQ 364

Query: 143 QVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLKNE---------------VKANKNTV 202
           QVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLKNE               + ANKNTV
Sbjct: 365 QVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLKNEGIDGSRVEKVLDWVHIAANKNTV 424

Query: 203 PGDVSAISPSGIRMGTPALTSRGFLEADFV--------------------------KDFE 242
           PGDVSAISPSGIRMGTPALTSRGFLEADFV                          KDFE
Sbjct: 425 PGDVSAISPSGIRMGTPALTSRGFLEADFVKVAEFYDEATKLALKIKAAEPKGAKLKDFE 484

BLAST of CmaCh12G001780 vs. ExPASy TrEMBL
Match: A0A6J1KJZ9 (Glycine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495956 PE=3 SV=1)

HSP 1 Score: 280.8 bits (717), Expect = 5.4e-72
Identity = 165/262 (62.98%), Postives = 172/262 (65.65%), Query Frame = 0

Query: 56  PNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE--------------------- 115
           P EY      +T  SL GPRGALIF+RKGVKEINKKGEE                     
Sbjct: 273 PFEYADIVTTTTNKSLRGPRGALIFFRKGVKEINKKGEEVLYDYEDKINSAVFPGFQGSP 332

Query: 116 --------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK 175
                         ATTPEYKAYQQQVLNNSS+FAQCLIEKGYELVSGGTDNHLVL+NLK
Sbjct: 333 HNHKIAGLAIALKQATTPEYKAYQQQVLNNSSKFAQCLIEKGYELVSGGTDNHLVLINLK 392

Query: 176 NE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADFV----- 235
            +               + ANKNTVPGDVSA+ P GIRMGTPALTSRGFLEADFV     
Sbjct: 393 IKGIDGSRLEKVLDWVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLEADFVKVAEF 452

Query: 236 ---------------------KDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE 242
                                KDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE
Sbjct: 453 FDEALKLALKIKAAEPKGAKLKDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE 512

BLAST of CmaCh12G001780 vs. ExPASy TrEMBL
Match: A0A6J1KRZ7 (Glycine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495956 PE=3 SV=1)

HSP 1 Score: 269.6 bits (688), Expect = 1.2e-68
Identity = 157/262 (59.92%), Postives = 169/262 (64.50%), Query Frame = 0

Query: 56  PNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE--------------------- 115
           P EY      +T  SL GPRGA+IF+RKGVKEINKKGEE                     
Sbjct: 270 PFEYADIVTTTTNKSLRGPRGAMIFFRKGVKEINKKGEEVLYDYEDKINNAVFPGVQGAS 329

Query: 116 --------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK 175
                         A TPEYKAYQQQVL NSS+ AQCLIEKGYELVSGGTDNHLVL++LK
Sbjct: 330 HNQKLAGLAISLKQAMTPEYKAYQQQVLRNSSKLAQCLIEKGYELVSGGTDNHLVLIDLK 389

Query: 176 NE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADF------ 235
           N+               + +NKNTVPGDVSA+ P GIR+GTPALTSRG LEADF      
Sbjct: 390 NKGIDGSRVEKVLEWVRIASNKNTVPGDVSAVIPGGIRLGTPALTSRGLLEADFAQVAEF 449

Query: 236 --------------------VKDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE 242
                               +KDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE
Sbjct: 450 FDEGLKLALKIKAAEPKGAKLKDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE 509

BLAST of CmaCh12G001780 vs. ExPASy TrEMBL
Match: A0A6J1GI73 (Glycine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454159 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 6.4e-65
Identity = 152/245 (62.04%), Postives = 158/245 (64.49%), Query Frame = 0

Query: 56  PNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE--------------------- 115
           P EY      +T  SLGGPRGALIFYRKGVKEINKKGEE                     
Sbjct: 304 PFEYADIVTTTTNKSLGGPRGALIFYRKGVKEINKKGEEVLYDYEDKINGAVFPGFQGSP 363

Query: 116 --------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK 175
                         ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHL+LVNLK
Sbjct: 364 HNHKIAGLAIALKQATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLLLVNLK 423

Query: 176 NE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADFV----- 225
           N+               + ANKNTVPGDVSA+SPSGIRMGTPALTSRGFLEADFV     
Sbjct: 424 NKGIDGSRVEKVLDWVHIAANKNTVPGDVSAVSPSGIRMGTPALTSRGFLEADFVKVAEF 483

BLAST of CmaCh12G001780 vs. ExPASy TrEMBL
Match: A0A6J1GH35 (Glycine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454159 PE=3 SV=1)

HSP 1 Score: 257.3 bits (656), Expect = 6.4e-65
Identity = 152/245 (62.04%), Postives = 158/245 (64.49%), Query Frame = 0

Query: 56  PNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE--------------------- 115
           P EY      +T  SLGGPRGALIFYRKGVKEINKKGEE                     
Sbjct: 272 PFEYADIVTTTTNKSLGGPRGALIFYRKGVKEINKKGEEVLYDYEDKINGAVFPGFQGSP 331

Query: 116 --------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK 175
                         ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHL+LVNLK
Sbjct: 332 HNHKIAGLAIALKQATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLLLVNLK 391

Query: 176 NE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADFV----- 225
           N+               + ANKNTVPGDVSA+SPSGIRMGTPALTSRGFLEADFV     
Sbjct: 392 NKGIDGSRVEKVLDWVHIAANKNTVPGDVSAVSPSGIRMGTPALTSRGFLEADFVKVAEF 451

BLAST of CmaCh12G001780 vs. NCBI nr
Match: XP_023001922.1 (serine hydroxymethyltransferase 2, mitochondrial-like isoform X2 [Cucurbita maxima])

HSP 1 Score: 297.4 bits (760), Expect = 1.1e-76
Identity = 178/297 (59.93%), Postives = 189/297 (63.64%), Query Frame = 0

Query: 23  KIKEKHVISWTSGQAVWSPKFFFFVEMISEGVEPNEYTLTSALSTCC--SLGGPRGALIF 82
           KI +KH       +A+          +++  V P+ +     ++T    SLGGPRGALIF
Sbjct: 245 KICDKH-------KAIMMADMAHLAGLVAADVIPSRFEYADIVTTTTNKSLGGPRGALIF 304

Query: 83  YRKGVKEINKKGEE-----------------------------------ATTPEYKAYQQ 142
           YRKGVKEINKKGEE                                   ATTPEYKAYQQ
Sbjct: 305 YRKGVKEINKKGEEVLYDYKDKINGTVFPGFQGSPHNHKIAGPVIALKQATTPEYKAYQQ 364

Query: 143 QVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLKNE---------------VKANKNTV 202
           QVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLKNE               + ANKNTV
Sbjct: 365 QVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLKNEGIDGSRVEKVLDWVHIAANKNTV 424

Query: 203 PGDVSAISPSGIRMGTPALTSRGFLEADFV--------------------------KDFE 242
           PGDVSAISPSGIRMGTPALTSRGFLEADFV                          KDFE
Sbjct: 425 PGDVSAISPSGIRMGTPALTSRGFLEADFVKVAEFYDEATKLALKIKAAEPKGAKLKDFE 484

BLAST of CmaCh12G001780 vs. NCBI nr
Match: XP_023001921.1 (serine hydroxymethyltransferase 2, mitochondrial-like isoform X1 [Cucurbita maxima])

HSP 1 Score: 280.8 bits (717), Expect = 1.1e-71
Identity = 165/262 (62.98%), Postives = 172/262 (65.65%), Query Frame = 0

Query: 56  PNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE--------------------- 115
           P EY      +T  SL GPRGALIF+RKGVKEINKKGEE                     
Sbjct: 273 PFEYADIVTTTTNKSLRGPRGALIFFRKGVKEINKKGEEVLYDYEDKINSAVFPGFQGSP 332

Query: 116 --------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK 175
                         ATTPEYKAYQQQVLNNSS+FAQCLIEKGYELVSGGTDNHLVL+NLK
Sbjct: 333 HNHKIAGLAIALKQATTPEYKAYQQQVLNNSSKFAQCLIEKGYELVSGGTDNHLVLINLK 392

Query: 176 NE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADFV----- 235
            +               + ANKNTVPGDVSA+ P GIRMGTPALTSRGFLEADFV     
Sbjct: 393 IKGIDGSRLEKVLDWVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFLEADFVKVAEF 452

Query: 236 ---------------------KDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE 242
                                KDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE
Sbjct: 453 FDEALKLALKIKAAEPKGAKLKDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE 512

BLAST of CmaCh12G001780 vs. NCBI nr
Match: XP_023001923.1 (serine hydroxymethyltransferase 2, mitochondrial-like isoform X3 [Cucurbita maxima])

HSP 1 Score: 269.6 bits (688), Expect = 2.6e-68
Identity = 157/262 (59.92%), Postives = 169/262 (64.50%), Query Frame = 0

Query: 56  PNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE--------------------- 115
           P EY      +T  SL GPRGA+IF+RKGVKEINKKGEE                     
Sbjct: 270 PFEYADIVTTTTNKSLRGPRGAMIFFRKGVKEINKKGEEVLYDYEDKINNAVFPGVQGAS 329

Query: 116 --------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK 175
                         A TPEYKAYQQQVL NSS+ AQCLIEKGYELVSGGTDNHLVL++LK
Sbjct: 330 HNQKLAGLAISLKQAMTPEYKAYQQQVLRNSSKLAQCLIEKGYELVSGGTDNHLVLIDLK 389

Query: 176 NE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADF------ 235
           N+               + +NKNTVPGDVSA+ P GIR+GTPALTSRG LEADF      
Sbjct: 390 NKGIDGSRVEKVLEWVRIASNKNTVPGDVSAVIPGGIRLGTPALTSRGLLEADFAQVAEF 449

Query: 236 --------------------VKDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE 242
                               +KDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE
Sbjct: 450 FDEGLKLALKIKAAEPKGAKLKDFEATMQLNKEFQSEISKLRREVEEFSKQFPTVGFEKE 509

BLAST of CmaCh12G001780 vs. NCBI nr
Match: XP_022951271.1 (serine hydroxymethyltransferase 2, mitochondrial-like isoform X3 [Cucurbita moschata])

HSP 1 Score: 257.3 bits (656), Expect = 1.3e-64
Identity = 152/245 (62.04%), Postives = 158/245 (64.49%), Query Frame = 0

Query: 56  PNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE--------------------- 115
           P EY      +T  SLGGPRGALIFYRKGVKEINKKGEE                     
Sbjct: 272 PFEYADIVTTTTNKSLGGPRGALIFYRKGVKEINKKGEEVLYDYEDKINGAVFPGFQGSP 331

Query: 116 --------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK 175
                         ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHL+LVNLK
Sbjct: 332 HNHKIAGLAIALKQATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLLLVNLK 391

Query: 176 NE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADFV----- 225
           N+               + ANKNTVPGDVSA+SPSGIRMGTPALTSRGFLEADFV     
Sbjct: 392 NKGIDGSRVEKVLDWVHIAANKNTVPGDVSAVSPSGIRMGTPALTSRGFLEADFVKVAEF 451

BLAST of CmaCh12G001780 vs. NCBI nr
Match: XP_022951269.1 (serine hydroxymethyltransferase 2, mitochondrial-like isoform X1 [Cucurbita moschata])

HSP 1 Score: 257.3 bits (656), Expect = 1.3e-64
Identity = 152/245 (62.04%), Postives = 158/245 (64.49%), Query Frame = 0

Query: 56  PNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE--------------------- 115
           P EY      +T  SLGGPRGALIFYRKGVKEINKKGEE                     
Sbjct: 304 PFEYADIVTTTTNKSLGGPRGALIFYRKGVKEINKKGEEVLYDYEDKINGAVFPGFQGSP 363

Query: 116 --------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK 175
                         ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHL+LVNLK
Sbjct: 364 HNHKIAGLAIALKQATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLLLVNLK 423

Query: 176 NE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADFV----- 225
           N+               + ANKNTVPGDVSA+SPSGIRMGTPALTSRGFLEADFV     
Sbjct: 424 NKGIDGSRVEKVLDWVHIAANKNTVPGDVSAVSPSGIRMGTPALTSRGFLEADFVKVAEF 483

BLAST of CmaCh12G001780 vs. TAIR 10
Match: AT5G26780.1 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 206.5 bits (524), Expect = 2.5e-53
Identity = 123/253 (48.62%), Postives = 150/253 (59.29%), Query Frame = 0

Query: 49  MISEGVEPN--EYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE------------ 108
           +++ GV P+  EY      +T  SL GPRGA+IF+RKG+KEINK+G+E            
Sbjct: 264 LVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGLKEINKQGKEVMYDYEDRINQA 323

Query: 109 -----------------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTD 168
                                  A TPEYKAYQ QVL N S+FA+ L+ KGY+LVSGGTD
Sbjct: 324 VFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVLRNCSKFAETLLAKGYDLVSGGTD 383

Query: 169 NHLVLVNLKNE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLE 225
           NHLVLVNLKN+               + ANKNTVPGDVSA+ P GIRMGTPALTSRGF+E
Sbjct: 384 NHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPGGIRMGTPALTSRGFIE 443

BLAST of CmaCh12G001780 vs. TAIR 10
Match: AT4G37930.1 (serine transhydroxymethyltransferase 1 )

HSP 1 Score: 202.2 bits (513), Expect = 4.7e-52
Identity = 120/244 (49.18%), Postives = 145/244 (59.43%), Query Frame = 0

Query: 56  PNEYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE--------------------- 115
           P +Y      +T  SL GPRGA+IF+RKGVKEINK+G+E                     
Sbjct: 273 PFDYADVVTTTTHKSLRGPRGAMIFFRKGVKEINKQGKEVLYDFEDKINQAVFPGLQGGP 332

Query: 116 --------------ATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK 175
                         ATT EYKAYQ+QVL+NS++FAQ L+E+GYELVSGGTDNHLVLVNLK
Sbjct: 333 HNHTITGLAVALKQATTSEYKAYQEQVLSNSAKFAQTLMERGYELVSGGTDNHLVLVNLK 392

Query: 176 NE---------------VKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADF------ 225
            +               + +NKNTVPGDVSA+ P GIRMGTPALTSRGF+E DF      
Sbjct: 393 PKGIDGSRVEKVLEAVHIASNKNTVPGDVSAMVPGGIRMGTPALTSRGFVEEDFAKVAEY 452

BLAST of CmaCh12G001780 vs. TAIR 10
Match: AT5G26780.3 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 197.2 bits (500), Expect = 1.5e-50
Identity = 124/269 (46.10%), Postives = 150/269 (55.76%), Query Frame = 0

Query: 49  MISEGVEPN--EYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE------------ 108
           +++ GV P+  EY      +T  SL GPRGA+IF+RKG+KEINK+G+E            
Sbjct: 264 LVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGLKEINKQGKEVMYDYEDRINQA 323

Query: 109 -----------------------ATTPEYKAYQQQVLNNSSRFA---------------- 168
                                  A TPEYKAYQ QVL N S+FA                
Sbjct: 324 VFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVLRNCSKFAELDIRPTVIISYGLSM 383

Query: 169 QCLIEKGYELVSGGTDNHLVLVNLKNE---------------VKANKNTVPGDVSAISPS 225
           Q L+ KGY+LVSGGTDNHLVLVNLKN+               + ANKNTVPGDVSA+ P 
Sbjct: 384 QTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPG 443

BLAST of CmaCh12G001780 vs. TAIR 10
Match: AT5G26780.2 (serine hydroxymethyltransferase 2 )

HSP 1 Score: 197.2 bits (500), Expect = 1.5e-50
Identity = 124/269 (46.10%), Postives = 150/269 (55.76%), Query Frame = 0

Query: 49  MISEGVEPN--EYTLTSALSTCCSLGGPRGALIFYRKGVKEINKKGEE------------ 108
           +++ GV P+  EY      +T  SL GPRGA+IF+RKG+KEINK+G+E            
Sbjct: 264 LVAAGVIPSPFEYADVVTTTTHKSLRGPRGAMIFFRKGLKEINKQGKEVMYDYEDRINQA 323

Query: 109 -----------------------ATTPEYKAYQQQVLNNSSRFA---------------- 168
                                  A TPEYKAYQ QVL N S+FA                
Sbjct: 324 VFPGLQGGPHNHTITGLAVALKQARTPEYKAYQDQVLRNCSKFAELDIRPTVIISYGLSM 383

Query: 169 QCLIEKGYELVSGGTDNHLVLVNLKNE---------------VKANKNTVPGDVSAISPS 225
           Q L+ KGY+LVSGGTDNHLVLVNLKN+               + ANKNTVPGDVSA+ P 
Sbjct: 384 QTLLAKGYDLVSGGTDNHLVLVNLKNKGIDGSRVEKVLELVHIAANKNTVPGDVSAMVPG 443

BLAST of CmaCh12G001780 vs. TAIR 10
Match: AT4G32520.1 (serine hydroxymethyltransferase 3 )

HSP 1 Score: 107.1 bits (266), Expect = 2.0e-23
Identity = 82/229 (35.81%), Postives = 114/229 (49.78%), Query Frame = 0

Query: 55  EPNEYTLTSALSTCCSLGGPRGALIFYRK----GV---KEINK------KG--------- 114
           +P EY      +T  SL GPRG +IF+RK    GV     +N       +G         
Sbjct: 300 DPFEYCDIVTTTTHKSLRGPRGGMIFFRKDPINGVDLESAVNNAVFPGLQGGPHNHTIGG 359

Query: 115 -----EEATTPEYKAYQQQVLNNSSRFAQCLIEKGYELVSGGTDNHLVLVNLK------- 174
                + A +PE+KAYQ++V++N    A  L+E G++LVSGG+DNHLVLV+L+       
Sbjct: 360 LAVCLKHAQSPEFKAYQKRVVSNCRALANRLVELGFKLVSGGSDNHLVLVDLRPMGMDGA 419

Query: 175 --------NEVKANKNTVPGDVSAISPSGIRMGTPALTSRGFLEADF--VKDF-----EA 216
                     +  NKN+VPGD SA+ P GIR+G+PA+T+RG  E DF  V DF     E 
Sbjct: 420 RVEKILDMASITLNKNSVPGDKSALVPGGIRIGSPAMTTRGLSEKDFVVVADFIKEGVEI 479

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P504336.4e-5449.01Serine hydroxymethyltransferase, mitochondrial OS=Solanum tuberosum OX=4113 PE=2... [more]
Q94C743.5e-5248.62Serine hydroxymethyltransferase 2, mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
P348993.9e-5147.22Serine hydroxymethyltransferase, mitochondrial OS=Pisum sativum OX=3888 PE=1 SV=... [more]
Q9SZJ56.6e-5149.18Serine hydroxymethyltransferase 1, mitochondrial OS=Arabidopsis thaliana OX=3702... [more]
P493581.1e-5047.04Serine hydroxymethyltransferase 2, mitochondrial OS=Flaveria pringlei OX=4226 PE... [more]
Match NameE-valueIdentityDescription
A0A6J1KHZ95.6e-7759.93Glycine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495956 PE=... [more]
A0A6J1KJZ95.4e-7262.98Glycine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495956 PE=... [more]
A0A6J1KRZ71.2e-6859.92Glycine hydroxymethyltransferase OS=Cucurbita maxima OX=3661 GN=LOC111495956 PE=... [more]
A0A6J1GI736.4e-6562.04Glycine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454159 P... [more]
A0A6J1GH356.4e-6562.04Glycine hydroxymethyltransferase OS=Cucurbita moschata OX=3662 GN=LOC111454159 P... [more]
Match NameE-valueIdentityDescription
XP_023001922.11.1e-7659.93serine hydroxymethyltransferase 2, mitochondrial-like isoform X2 [Cucurbita maxi... [more]
XP_023001921.11.1e-7162.98serine hydroxymethyltransferase 2, mitochondrial-like isoform X1 [Cucurbita maxi... [more]
XP_023001923.12.6e-6859.92serine hydroxymethyltransferase 2, mitochondrial-like isoform X3 [Cucurbita maxi... [more]
XP_022951271.11.3e-6462.04serine hydroxymethyltransferase 2, mitochondrial-like isoform X3 [Cucurbita mosc... [more]
XP_022951269.11.3e-6462.04serine hydroxymethyltransferase 2, mitochondrial-like isoform X1 [Cucurbita mosc... [more]
Match NameE-valueIdentityDescription
AT5G26780.12.5e-5348.62serine hydroxymethyltransferase 2 [more]
AT4G37930.14.7e-5249.18serine transhydroxymethyltransferase 1 [more]
AT5G26780.31.5e-5046.10serine hydroxymethyltransferase 2 [more]
AT5G26780.21.5e-5046.10serine hydroxymethyltransferase 2 [more]
AT4G32520.12.0e-2335.81serine hydroxymethyltransferase 3 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 183..210
NoneNo IPR availablePANTHERPTHR11680:SF49SERINE HYDROXYMETHYLTRANSFERASEcoord: 51..94
coord: 180..224
NoneNo IPR availablePANTHERPTHR11680:SF49SERINE HYDROXYMETHYLTRANSFERASEcoord: 94..181
IPR039429Serine hydroxymethyltransferase-like domainPFAMPF00464SHMTcoord: 94..181
e-value: 1.6E-28
score: 99.4
IPR015422Pyridoxal phosphate-dependent transferase, small domainGENE3D3.90.1150.10Aspartate Aminotransferase, domain 1coord: 97..219
e-value: 3.9E-35
score: 122.9
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 94..181
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 51..94
IPR001085Serine hydroxymethyltransferasePANTHERPTHR11680SERINE HYDROXYMETHYLTRANSFERASEcoord: 180..224
IPR015424Pyridoxal phosphate-dependent transferaseSUPERFAMILY53383PLP-dependent transferasescoord: 66..215

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G001780.1CmaCh12G001780.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0019264 glycine biosynthetic process from serine
biological_process GO:0035999 tetrahydrofolate interconversion
molecular_function GO:0004372 glycine hydroxymethyltransferase activity
molecular_function GO:0030170 pyridoxal phosphate binding
molecular_function GO:0003824 catalytic activity