CmaCh12G001180 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G001180
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description1-phosphatidylinositol 4-kinase
LocationCma_Chr12: 484630 .. 487658 (+)
RNA-Seq ExpressionCmaCh12G001180
SyntenyCmaCh12G001180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGAACGGAGAAGGAACTGTTCTTTGCCTCATTACGAGGATCTTTATTTTGATTCTTCTTATTATACCTCTGCAGATTCTCACAGTTGAATGACGCATTCATTGATATGAGCTACGTTGTTTGATTTTCGCGCTTCTCATCTCAGATCTCTAGTTCGAGTGTGAGTTTTCTCCTTCTGATTTCGCTGTGTGTTTGTCTGTTCGTTGATTTGTTTTGTTTTTGTTGCTAATTCATCGTTGGATTAATCAATGTCGTTTTGGTGGAATTTCACGTCGAGTCTCAGAATTGTGTTTGTGGTAATTTCTCTTAAGATTTTGGGATGAAATATGTTGTGACATGTGTTGATTTTGGATTTGGGGAGCTGGTGGTGTGGAATGAGGAATAATTGTTTCTGCAGCATTGCGTGTGAGATGATACTAGTATTATGGTTGTTTTTTATTATTTCTTTACGAGTCTTCAGAGACCAATCTGGATTTAGACCGACTTATTGAAATAATTGAGCTGCGAATGGTGCGTTTTCTTTACTCTGCATCGGTTGCATTTAATTCTGAAGTTATTTAGCTTTTTATGAGGACTGGCAGTGGAGTTTCTCAACTACCAGAGTCTCGAGAAGTTGTGAAATTTACTGATCTGTGATATTTGCATTCATTGAGACTAATACAATGTTCAGAAGTTAGGATTTTACTTTCTTTCCCGGAAACAGAAATAAATGTCCTTTTCTTTGTTGAAATCGATTAAACCCTTTTTTTTGTGATGATTATTATTATTTTTTTTGCTCCGAGGTTGTAAATATAGTGGCCCTTGCACCGTACTGGAATTTATACATGATGTGTTCTAGCTGCCATGAATTAACTTTTTGTAATAAAATTTGATATTGGGTTTCTTTGGCAGATCTTGGGCTCTTCATATCCTGCTGAATGGTTATGTATTTGCATTCCCTTGGTTTCTTGTGGGCATTCCTTCGTTGAGAATCTTGAGTGGCATTTCGTTACTGTATGAACGTTAATGATTTTTAGGGTACAAGAATTTGTTGTTGCTCATTCAGCATCGGCTATAGAGTTGGGCACTTTTCTGGAAGAGAAGGAACTCTCTTCCCTTGCCACCTCTCTTGCCTGCTGCCAACACATTTGTATTTAATGCATCGTAAATTGGACAGTCCAGTCCAGACGCAAATGGCGGTGGCGGCAGCCTTTACGAGTCCTCTCAGTGGTGTAGAATATGGCGGCAGCAAGAGGATGGTAGGAAGGCAGGCTACAGGGAGGAGAAGGGTCTTTGTGCAAACCGACACTGGTTGTGTCCTGGGGATGGAATTAGATTGGAGTGATAATGCTCATACTGTGAAGAGGAGGTTGCAGCTTGCGCTCAATGTACCCACTGATGAGAGCTCTTTGACATTTGGTGATACGGTGCTCAAGAACGACCTCAGTGGCGTACGAAATGATTCCCCACTTCTTCTTACTCGAAACATTTTGCACAGAAGCTCGTCAACTCCATGTCTCTCGCCTACTGGAAGAGATATCCAGCAAAGAGATCAGAGTGGCCCTATTGAGATATTGGGGCATTCTGATTGCTTTGTTAGGATAAAACAACTGGTTTGCGAGATTATAAAAGCTACTAAAATTGGTGTAGATCCTCTTCCTGTTCATAGTGGACTTGGTGGTGCATACTATTTCAGAAATAACAGAGGTGAGAGCGTTGCGATTGTGAAGCCTACTGATGAAGAACCTTTTGCACCAAACAATCCAAAAGGGTATGTTGGTAAAGCGTTAGGTGAACCTGGTTTAAAACGATCAGTGCGTGTTGGAGAGACTGGATTTCGCGAGGTTGCAGCTTATCTTCTCGACTATAATCACTTTGCTAATGTGCCTGCCACTGCCCTGGTGAAGATTACGCACTCGATCTTCAATGTCAATGATGGAGTAAATGGTAACAAGTCAAGCAAGAACAAGTTGGTTAGCAAGATTGCATCGTTCCAAGAATTCATACCACATGACTTTGATGCCAGTGATTACGGGACTTCCAGCTTCCCAGTCGTGGGTGTTCATCGTATAGGCATTCTAGACATTAGAGTTTTTAACACAGATAGGCATGGTGGAAATCTTTTAGTTAGGAAACTTGATGGTGTTGGGATGTTTGGTCAAGTGGAACTTATTCCAATTGATCATGGACTCTGCCTGCCTGAAACATTAGAGGATCCATATTTCGAGTGGATTCATTGGCCACAGGCTTCAATTCCTTTCTCAGAAGATGAGCTCAAGTATATCAAAGATCTCAATCCATTTAAAGATAGTGAAATGCTCAGGATGGAGCTTCCCATGATCCGTGAGGGTTGTCTTAGAGTTCTGATTCTCTGCACTATCTTCCTCAAGGAAGCTGCTGCATTTGGTCTCTGTCTTGCTGAAATTGGCGAGATGATGACTAGAGAGTTTCACAGCGGAGAGGAGGATCCCAGTGAACTGGAACTCATATGCATCAAGGCTAGGCAGCTGATCGATGAAAGGGAGGTCTTATCTCCCCAGGCTGATGATTTAAGGGATGAGGGATTTCAGTTTGATATAGACTGCGATGAAATGGAGTCAGGCTTTACCCAAGAGGTAGAATCCGATGATTTTTTCCCTACAACGCCATTTCCTTTCGGGATCGAGGAAGAAAACGGTGAGGAAGACGAGCGAGGGGGCTACCTCGATTTGTTTACTTCCAAAAGGATTCCAACTATTTCAAAGCTTTCAATGTCACTGAAGAATACCAATCTAGGTGAGAAGAATAAGAAACATGCGAAGTACTTTGGAACAAGACCTGGGAACGGATACATAGTGACCAATGCATCATCCTCTGGGCGCAGGAGTGCAAATGAACAGATTCCAGCCAGTGTAACTTTTGTGAAGCTAGCAGACATGAATGAGGAAGTTTGGTCATTGTTTTTAGAGAAGTTTCAAGAGCTACTTCCCCCGGCATTTGCTAAGCGAAAATCCGCCACTCTTGGTCAATGGCAGAGGCAAAGGCTCGGCACTTCTTGCCAATTTTGA

mRNA sequence

ATGGAGAACGGAGAAGGAACTATCTCTAGTTCGAGTATCTTGGGCTCTTCATATCCTGCTGAATGGTTATGTATTTGCATTCCCTTGGGTACAAGAATTTGTTGTTGCTCATTCAGCATCGGCTATAGAGTTGGGCACTTTTCTGGAAGAGAAGGAACTCTCTTCCCTTGCCACCTCTCTTGCCTGCTGCCAACACATTTGTATTTAATGCATCGTAAATTGGACAGTCCAGTCCAGACGCAAATGGCGGTGGCGGCAGCCTTTACGAGTCCTCTCAGTGGTGTAGAATATGGCGGCAGCAAGAGGATGGTAGGAAGGCAGGCTACAGGGAGGAGAAGGGTCTTTGTGCAAACCGACACTGGTTGTGTCCTGGGGATGGAATTAGATTGGAGTGATAATGCTCATACTGTGAAGAGGAGGTTGCAGCTTGCGCTCAATGTACCCACTGATGAGAGCTCTTTGACATTTGGTGATACGGTGCTCAAGAACGACCTCAGTGGCGTACGAAATGATTCCCCACTTCTTCTTACTCGAAACATTTTGCACAGAAGCTCGTCAACTCCATGTCTCTCGCCTACTGGAAGAGATATCCAGCAAAGAGATCAGAGTGGCCCTATTGAGATATTGGGGCATTCTGATTGCTTTGTTAGGATAAAACAACTGGTTTGCGAGATTATAAAAGCTACTAAAATTGGTGTAGATCCTCTTCCTGTTCATAGTGGACTTGGTGGTGCATACTATTTCAGAAATAACAGAGGTGAGAGCGTTGCGATTGTGAAGCCTACTGATGAAGAACCTTTTGCACCAAACAATCCAAAAGGGTATGTTGGTAAAGCGTTAGGTGAACCTGGTTTAAAACGATCAGTGCGTGTTGGAGAGACTGGATTTCGCGAGGTTGCAGCTTATCTTCTCGACTATAATCACTTTGCTAATGTGCCTGCCACTGCCCTGGTGAAGATTACGCACTCGATCTTCAATGTCAATGATGGAGTAAATGGTAACAAGTCAAGCAAGAACAAGTTGGTTAGCAAGATTGCATCGTTCCAAGAATTCATACCACATGACTTTGATGCCAGTGATTACGGGACTTCCAGCTTCCCAGTCGTGGGTGTTCATCGTATAGGCATTCTAGACATTAGAGTTTTTAACACAGATAGGCATGGTGGAAATCTTTTAGTTAGGAAACTTGATGGTGTTGGGATGTTTGGTCAAGTGGAACTTATTCCAATTGATCATGGACTCTGCCTGCCTGAAACATTAGAGGATCCATATTTCGAGTGGATTCATTGGCCACAGGCTTCAATTCCTTTCTCAGAAGATGAGCTCAAGTATATCAAAGATCTCAATCCATTTAAAGATAGTGAAATGCTCAGGATGGAGCTTCCCATGATCCGTGAGGGTTGTCTTAGAGTTCTGATTCTCTGCACTATCTTCCTCAAGGAAGCTGCTGCATTTGGTCTCTGTCTTGCTGAAATTGGCGAGATGATGACTAGAGAGTTTCACAGCGGAGAGGAGGATCCCAGTGAACTGGAACTCATATGCATCAAGGCTAGGCAGCTGATCGATGAAAGGGAGGTCTTATCTCCCCAGGCTGATGATTTAAGGGATGAGGGATTTCAGTTTGATATAGACTGCGATGAAATGGAGTCAGGCTTTACCCAAGAGGTAGAATCCGATGATTTTTTCCCTACAACGCCATTTCCTTTCGGGATCGAGGAAGAAAACGGTGAGGAAGACGAGCGAGGGGGCTACCTCGATTTGTTTACTTCCAAAAGGATTCCAACTATTTCAAAGCTTTCAATGTCACTGAAGAATACCAATCTAGGTGAGAAGAATAAGAAACATGCGAAGTACTTTGGAACAAGACCTGGGAACGGATACATAGTGACCAATGCATCATCCTCTGGGCGCAGGAGTGCAAATGAACAGATTCCAGCCAGTGTAACTTTTGTGAAGCTAGCAGACATGAATGAGGAAGTTTGGTCATTGTTTTTAGAGAAGTTTCAAGAGCTACTTCCCCCGGCATTTGCTAAGCGAAAATCCGCCACTCTTGGTCAATGGCAGAGGCAAAGGCTCGGCACTTCTTGCCAATTTTGA

Coding sequence (CDS)

ATGGAGAACGGAGAAGGAACTATCTCTAGTTCGAGTATCTTGGGCTCTTCATATCCTGCTGAATGGTTATGTATTTGCATTCCCTTGGGTACAAGAATTTGTTGTTGCTCATTCAGCATCGGCTATAGAGTTGGGCACTTTTCTGGAAGAGAAGGAACTCTCTTCCCTTGCCACCTCTCTTGCCTGCTGCCAACACATTTGTATTTAATGCATCGTAAATTGGACAGTCCAGTCCAGACGCAAATGGCGGTGGCGGCAGCCTTTACGAGTCCTCTCAGTGGTGTAGAATATGGCGGCAGCAAGAGGATGGTAGGAAGGCAGGCTACAGGGAGGAGAAGGGTCTTTGTGCAAACCGACACTGGTTGTGTCCTGGGGATGGAATTAGATTGGAGTGATAATGCTCATACTGTGAAGAGGAGGTTGCAGCTTGCGCTCAATGTACCCACTGATGAGAGCTCTTTGACATTTGGTGATACGGTGCTCAAGAACGACCTCAGTGGCGTACGAAATGATTCCCCACTTCTTCTTACTCGAAACATTTTGCACAGAAGCTCGTCAACTCCATGTCTCTCGCCTACTGGAAGAGATATCCAGCAAAGAGATCAGAGTGGCCCTATTGAGATATTGGGGCATTCTGATTGCTTTGTTAGGATAAAACAACTGGTTTGCGAGATTATAAAAGCTACTAAAATTGGTGTAGATCCTCTTCCTGTTCATAGTGGACTTGGTGGTGCATACTATTTCAGAAATAACAGAGGTGAGAGCGTTGCGATTGTGAAGCCTACTGATGAAGAACCTTTTGCACCAAACAATCCAAAAGGGTATGTTGGTAAAGCGTTAGGTGAACCTGGTTTAAAACGATCAGTGCGTGTTGGAGAGACTGGATTTCGCGAGGTTGCAGCTTATCTTCTCGACTATAATCACTTTGCTAATGTGCCTGCCACTGCCCTGGTGAAGATTACGCACTCGATCTTCAATGTCAATGATGGAGTAAATGGTAACAAGTCAAGCAAGAACAAGTTGGTTAGCAAGATTGCATCGTTCCAAGAATTCATACCACATGACTTTGATGCCAGTGATTACGGGACTTCCAGCTTCCCAGTCGTGGGTGTTCATCGTATAGGCATTCTAGACATTAGAGTTTTTAACACAGATAGGCATGGTGGAAATCTTTTAGTTAGGAAACTTGATGGTGTTGGGATGTTTGGTCAAGTGGAACTTATTCCAATTGATCATGGACTCTGCCTGCCTGAAACATTAGAGGATCCATATTTCGAGTGGATTCATTGGCCACAGGCTTCAATTCCTTTCTCAGAAGATGAGCTCAAGTATATCAAAGATCTCAATCCATTTAAAGATAGTGAAATGCTCAGGATGGAGCTTCCCATGATCCGTGAGGGTTGTCTTAGAGTTCTGATTCTCTGCACTATCTTCCTCAAGGAAGCTGCTGCATTTGGTCTCTGTCTTGCTGAAATTGGCGAGATGATGACTAGAGAGTTTCACAGCGGAGAGGAGGATCCCAGTGAACTGGAACTCATATGCATCAAGGCTAGGCAGCTGATCGATGAAAGGGAGGTCTTATCTCCCCAGGCTGATGATTTAAGGGATGAGGGATTTCAGTTTGATATAGACTGCGATGAAATGGAGTCAGGCTTTACCCAAGAGGTAGAATCCGATGATTTTTTCCCTACAACGCCATTTCCTTTCGGGATCGAGGAAGAAAACGGTGAGGAAGACGAGCGAGGGGGCTACCTCGATTTGTTTACTTCCAAAAGGATTCCAACTATTTCAAAGCTTTCAATGTCACTGAAGAATACCAATCTAGGTGAGAAGAATAAGAAACATGCGAAGTACTTTGGAACAAGACCTGGGAACGGATACATAGTGACCAATGCATCATCCTCTGGGCGCAGGAGTGCAAATGAACAGATTCCAGCCAGTGTAACTTTTGTGAAGCTAGCAGACATGAATGAGGAAGTTTGGTCATTGTTTTTAGAGAAGTTTCAAGAGCTACTTCCCCCGGCATTTGCTAAGCGAAAATCCGCCACTCTTGGTCAATGGCAGAGGCAAAGGCTCGGCACTTCTTGCCAATTTTGA

Protein sequence

MENGEGTISSSSILGSSYPAEWLCICIPLGTRICCCSFSIGYRVGHFSGREGTLFPCHLSCLLPTHLYLMHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELDWSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPCLSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFRNNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHFANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVVGVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIHWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCLAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEMESGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF
Homology
BLAST of CmaCh12G001180 vs. ExPASy Swiss-Prot
Match: Q9SI52 (Phosphatidylinositol 4-kinase gamma 7 OS=Arabidopsis thaliana OX=3702 GN=PI4KG7 PE=1 SV=2)

HSP 1 Score: 876.7 bits (2264), Expect = 1.7e-253
Identity = 446/660 (67.58%), Postives = 529/660 (80.15%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSK-----RMVGRQATGRRRVFVQTDTGCVL 129
           M R LDSPVQTQMAV A F +PL+G      K     + + RQ++G RRVFVQT+TGCVL
Sbjct: 1   MSRNLDSPVQTQMAV-AVFKTPLTGASKMEGKQHHKHQHLQRQSSG-RRVFVQTETGCVL 60

Query: 130 GMELDWSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRS 189
           GMELD SDN HTVKRRLQ+ALN PT+ESSLT+GD VL NDLS VRNDSPLLL RN +HRS
Sbjct: 61  GMELDRSDNVHTVKRRLQIALNFPTEESSLTYGDMVLTNDLSAVRNDSPLLLKRNFMHRS 120

Query: 190 SSTPCLSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGG 249
           SSTPCLSPTGRD+QQ+D+SGPIEILGHSDCF  +K +V +I+KA K+GV+PLPVHSGLGG
Sbjct: 121 SSTPCLSPTGRDLQQKDRSGPIEILGHSDCFSIVKHMVKDIVKAMKMGVEPLPVHSGLGG 180

Query: 250 AYYFRNNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLL 309
           AYYFRN RGESVAIVKPTDEEPFAPNNPKG+VGKALG+PGLK SVRVGETGFREVAAYLL
Sbjct: 181 AYYFRNKRGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKSSVRVGETGFREVAAYLL 240

Query: 310 DYNHFANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTS 369
           DY  FANVP TALVKITHS+FNVNDGV GNK  + KLVSKIASFQ+F+ HDFDASD+GTS
Sbjct: 241 DYGRFANVPPTALVKITHSVFNVNDGVKGNKPREKKLVSKIASFQKFVAHDFDASDHGTS 300

Query: 370 SFPVVGVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPY 429
           SFPV  VHRIGILDIR+FNTDRHGGNLLV+KLDGVGMFGQVELIPIDHGLCLPETLEDPY
Sbjct: 301 SFPVTSVHRIGILDIRIFNTDRHGGNLLVKKLDGVGMFGQVELIPIDHGLCLPETLEDPY 360

Query: 430 FEWIHWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAA 489
           FEWIHWPQAS+PFS++E+ YI+ L+P KD +MLR ELPMIRE CLRVL+LCTIFLKEA+A
Sbjct: 361 FEWIHWPQASLPFSDEEVDYIQSLDPVKDCDMLRRELPMIREACLRVLVLCTIFLKEASA 420

Query: 490 FGLCLAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDID 549
           +GLCLAEIGEMMTREF  GEE+PSELE++CI+A++ + ER+V SP++D + +  FQFD+D
Sbjct: 421 YGLCLAEIGEMMTREFRPGEEEPSELEVVCIEAKRSVTERDVFSPRSDVVGEAEFQFDLD 480

Query: 550 CDEMESGFTQEVE-SDDFFPTTPFPFG--------------IEEENGEEDERGGYLDLFT 609
           CD++ES ++ +++ +DD+F   PF  G               E+E  EED+    + +  
Sbjct: 481 CDDLESVYSSKIQLTDDYFTKNPFSNGRSSLGKLEESIKEEEEDEEEEEDKTENTVPMII 540

Query: 610 SK-----------RIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRR 669
            K           + P++SKLS S+KNT+L +  +K+ K   TR       +  +SSG +
Sbjct: 541 MKDSFFSSAAFHDKAPSLSKLSTSMKNTHLSDTTRKNPKPL-TRG-----KSENTSSGHK 600

Query: 670 SANEQIPASVTFVKLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           SANEQ+P S +FVK+ADM E+ W LFLE+FQELL PAFAKRK+ATL   +RQRLGTSCQF
Sbjct: 601 SANEQLPVSASFVKVADMKEDEWVLFLERFQELLGPAFAKRKTATLS--KRQRLGTSCQF 650

BLAST of CmaCh12G001180 vs. ExPASy Swiss-Prot
Match: Q8W4R8 (Phosphatidylinositol 4-kinase gamma 6 OS=Arabidopsis thaliana OX=3702 GN=PI4KG6 PE=2 SV=1)

HSP 1 Score: 819.3 bits (2115), Expect = 3.3e-236
Identity = 422/638 (66.14%), Postives = 503/638 (78.84%), Query Frame = 0

Query: 86  AAFTSPLSGVEYGGSKRMVGR---------QATGRRRVFVQTDTGCVLGMELDWSDNAHT 145
           A F +PL G E+ G+++M G+         Q+TGRRRVFVQTDTGCVLG+ELD +DN HT
Sbjct: 4   AVFKAPLKG-EFHGARKMEGKQYKHHLLQQQSTGRRRVFVQTDTGCVLGVELDRNDNVHT 63

Query: 146 VKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPCLSPTGRD 205
           VK+RLQ+A N PT+ESSLTFGD VLKNDLS VRNDSPLLL RN++HRSSSTPCLSPTG D
Sbjct: 64  VKKRLQIAFNFPTEESSLTFGDMVLKNDLSAVRNDSPLLLKRNLMHRSSSTPCLSPTGND 123

Query: 206 IQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFRNNRGESV 265
           +Q++D+SGPIEIL HS CF+ +KQ   +I+KA K+GV+P+PV+ GLGGAYYFR+ +G+SV
Sbjct: 124 LQRKDRSGPIEILSHSPCFLSLKQTANDIVKAMKMGVEPIPVNGGLGGAYYFRDEKGQSV 183

Query: 266 AIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHFANVPATA 325
           AIVKPTDEEPFAPNNPKG+VGKALG+PGLK SVRVGETGFREVAAYLLDY+HFANVP TA
Sbjct: 184 AIVKPTDEEPFAPNNPKGFVGKALGQPGLKPSVRVGETGFREVAAYLLDYDHFANVPPTA 243

Query: 326 LVKITHSIFNVNDGVNGNKS-SKNKLV-SKIASFQEFIPHDFDASDYGTSSFPVVGVHRI 385
           LVKITHS+FNVNDG++GNKS  K KLV SKIASFQ+F+PHDFDASD+GTSSFPV  VHRI
Sbjct: 244 LVKITHSVFNVNDGMDGNKSREKKKLVSSKIASFQKFVPHDFDASDHGTSSFPVASVHRI 303

Query: 386 GILDIRVFNTDRHGGNLLVRKLD--GVGMFGQVELIPIDHGLCLPETLEDPYFEWIHWPQ 445
           GILDIR+ NTDRHGGNLLV+KLD  GVG FGQVELIPIDHGLCLPETLEDPYFEWIHWPQ
Sbjct: 304 GILDIRILNTDRHGGNLLVKKLDDGGVGRFGQVELIPIDHGLCLPETLEDPYFEWIHWPQ 363

Query: 446 ASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCLAEI 505
           ASIPFSE+EL YI+ L+P KD EMLR ELPMIRE CLRVL+LCT+FLKEAA FGLCLAEI
Sbjct: 364 ASIPFSEEELDYIQSLDPVKDCEMLRRELPMIREACLRVLVLCTVFLKEAAVFGLCLAEI 423

Query: 506 GEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEMESGF 565
           GEMMTREF +GEE+PSELE++CI+A++L  E++VLSP++D   +  FQFDID +E++S +
Sbjct: 424 GEMMTREFRAGEEEPSELEMLCIEAKRLTTEQDVLSPKSDGEGETEFQFDIDYNELDSVY 483

Query: 566 TQEVESDDFFPTTPFPFG------IEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNT 625
             E E+D+FF   PF  G      ++E   EE+E     D   +K   ++SKLS S+KN 
Sbjct: 484 GSETETDEFFAKNPFSNGRSSLGELKESIAEEEED----DEEEAKLTLSLSKLSTSMKNN 543

Query: 626 NLGEKNKKHAKYFGTRPGNGYI------VTNASSSGRRSANEQIPASVTFVKLADMNEEV 685
                            G+GY+       T+ +    +SAN Q+P SV FVKLADM E  
Sbjct: 544 ------------LSNTMGSGYLKPPKDNQTDKALVSHKSANVQLPLSVNFVKLADMKEVE 603

Query: 686 WSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           W +FLE+FQELL  AFA+RK+ TL     QRLGTSC+F
Sbjct: 604 WVVFLERFQELLYSAFAERKTMTLR--NTQRLGTSCKF 622

BLAST of CmaCh12G001180 vs. ExPASy Swiss-Prot
Match: Q9C671 (Phosphatidylinositol 4-kinase gamma 5 OS=Arabidopsis thaliana OX=3702 GN=PI4KG5 PE=1 SV=1)

HSP 1 Score: 818.5 bits (2113), Expect = 5.6e-236
Identity = 430/647 (66.46%), Postives = 508/647 (78.52%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           M RKLDSP++TQMAVA    SPL+G E+    ++  +   GRRRVFVQT+TGCVLG+ELD
Sbjct: 1   MSRKLDSPIKTQMAVALV-KSPLNG-EFREFNKVGMKTPVGRRRVFVQTETGCVLGLELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
            SDNAHTVKR+LQ+ALN P +ESSLTFGD VLKNDL+ VR+DSPLLLTRN  HRSSSTPC
Sbjct: 61  RSDNAHTVKRKLQVALNFPIEESSLTFGDLVLKNDLTAVRSDSPLLLTRNNFHRSSSTPC 120

Query: 190 LSPTGRDIQQ-RDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYF 249
           LSP   D+QQ RD+S PIEILG+S  F  ++Q+  +I KA K G+DP+ V+SGLGGAYYF
Sbjct: 121 LSPMRADLQQRRDESSPIEILGNSVSFSFVRQMAKDITKAVKKGIDPVAVNSGLGGAYYF 180

Query: 250 RNNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNH 309
           +N+RGESVAIVKPTDEEP+APNNPKG+VGKALG+PGLKRSVRVGETG+REVAAYLLD  H
Sbjct: 181 KNSRGESVAIVKPTDEEPYAPNNPKGFVGKALGQPGLKRSVRVGETGYREVAAYLLDKEH 240

Query: 310 FANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPV 369
           FANVP TALVKITHSIFNVNDGV  +K  +  LVSKIAS Q+FIPHD+DAS++GTS+FPV
Sbjct: 241 FANVPPTALVKITHSIFNVNDGVKASKPMEKMLVSKIASLQQFIPHDYDASEHGTSNFPV 300

Query: 370 VGVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWI 429
             VHRIGILDIR+ NTDRH GNLLV+KLDG GMFGQVEL+PIDHGLCLPETLEDPYFEWI
Sbjct: 301 SAVHRIGILDIRILNTDRHSGNLLVKKLDGDGMFGQVELVPIDHGLCLPETLEDPYFEWI 360

Query: 430 HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLC 489
           HWPQASIPFSEDELKYI +L+P  D EMLR ELPM+RE  LRVL+LCTIFLKEAAA GLC
Sbjct: 361 HWPQASIPFSEDELKYIANLDPLGDCEMLRRELPMVREASLRVLVLCTIFLKEAAANGLC 420

Query: 490 LAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEM 549
           LAEIGEMMTRE   G+E+PSE+E++C++A  LI E++  SP++D   D  FQFDIDC+E+
Sbjct: 421 LAEIGEMMTREVRPGDEEPSEIEVVCLEAMSLIGEKDAESPRSDLGNDIEFQFDIDCEEV 480

Query: 550 ES-----------------GFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSK 609
                              G   +VE      TT      EEE+ EE+E     D    +
Sbjct: 481 TDCTKKLALPLGLTFGNARGQLSKVEE-----TTEDGEEEEEEDREEEEN----DRADLE 540

Query: 610 RIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFV 669
           ++PTI KLSMSLK+T LGEK++K+ K+ G R  + Y     +SS  RSA+EQIP+S +FV
Sbjct: 541 KMPTI-KLSMSLKSTLLGEKSQKYQKHPGARVESAY-----ASSAHRSADEQIPSSTSFV 600

Query: 670 KLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           KL+DM+EE W++FLEK+QELL PA  KRKS TLGQ QRQRLGTSCQF
Sbjct: 601 KLSDMSEEEWTIFLEKYQELLYPAIEKRKSITLGQKQRQRLGTSCQF 630

BLAST of CmaCh12G001180 vs. ExPASy Swiss-Prot
Match: Q0WMZ6 (Phosphatidylinositol 4-kinase gamma 8 OS=Arabidopsis thaliana OX=3702 GN=PI4KG8 PE=2 SV=2)

HSP 1 Score: 367.5 bits (942), Expect = 3.4e-100
Identity = 225/542 (41.51%), Postives = 303/542 (55.90%), Query Frame = 0

Query: 177 TRNILHRSSSTPCLSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPL 236
           + ++ +RS STPC S +G ++     +  IEILG       ++ LV E+  A   G  PL
Sbjct: 48  SHSLNNRSVSTPCFSISGSNL-DGSATPHIEILG-GQRVPTVRSLVAEVTIAIVSGAQPL 107

Query: 237 PVHSGLGGAYYFRNNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGF 296
            + SGLGGAY  +  +G ++A+ KP DEEP A NNPKG  G  LG+PG+KRS+RVGE+G 
Sbjct: 108 LLPSGLGGAYLLQTEKGNNIAVAKPVDEEPLAFNNPKGSGGLTLGQPGMKRSIRVGESGI 167

Query: 297 REVAAYLLDYNHFANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDF 356
           RE+AAYLLD+  F++VP TALV+I+H  F        +    +    K+AS Q F+ HDF
Sbjct: 168 RELAAYLLDHQGFSSVPPTALVRISHVPF--------HDRGSDHAAYKVASLQRFVGHDF 227

Query: 357 DASDYGTSSFPVVGVHRIGILDIRVFNTDRHGGNLLVRKL---------DGVGMFGQVEL 416
           DA + G  SF VV VHRIGILD+RV N DRH GN+LV+K+         +GV   G  EL
Sbjct: 228 DAGELGPGSFTVVSVHRIGILDVRVLNLDRHAGNMLVKKIHDQDETTCSNGV---GAAEL 287

Query: 417 IPIDHGLCLPETLEDPYFEWIHWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREG 476
           +PIDHGLCLPE L+DPYFEW++WPQAS+PF++ EL+YI +L+PFKD+E+LR EL  I+E 
Sbjct: 288 VPIDHGLCLPECLDDPYFEWLNWPQASVPFTDIELQYISNLDPFKDAELLRTELDSIQES 347

Query: 477 CLRVLILCTIFLKEAAAFGLCLAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVL 536
            LRVLI+CTIFLKEAAA GL LAEIGE MTR+   GEE  S LE++C KA+         
Sbjct: 348 SLRVLIVCTIFLKEAAAAGLSLAEIGEKMTRDICRGEESSSVLEILCNKAKASAVSG--- 407

Query: 537 SPQADDLRDEGFQFDIDCDEMESGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDL 596
           S   DD   E  + + + +     F  EVE  +       P                  +
Sbjct: 408 SDDDDDYSSEWNEVEAELECGIFQFDDEVECKELPDMLQVP------------------I 467

Query: 597 FTSKRIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQI--- 656
           FT  R+P+I+    +L                   P N +I T  ++      +  I   
Sbjct: 468 FT--RVPSIAANLSALMRC----------------PPNQWISTYDTNIEEERRDRSIVRS 527

Query: 657 ---PASVTF-----VKLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSC 699
              P  V +     V   DM+ + W +FL  FQ LLP A       +  +  + R G+SC
Sbjct: 528 KSHPICVNYDEKEGVYFGDMSGDEWEMFLHSFQMLLPEAL----EGSTSKGPKPRFGSSC 533

BLAST of CmaCh12G001180 vs. ExPASy Swiss-Prot
Match: O22199 (Phosphatidylinositol 4-kinase gamma 1 OS=Arabidopsis thaliana OX=3702 GN=PI4KG1 PE=2 SV=1)

HSP 1 Score: 364.0 bits (933), Expect = 3.8e-99
Identity = 224/529 (42.34%), Postives = 292/529 (55.20%), Query Frame = 0

Query: 181 LHRSSSTPCLSPTGRDIQQRDQSGP----IEILGHSDCFVRIKQLVCEIIKATKIGVDPL 240
           +HRS S+PC S       + D        IEILG       ++ LV E+  A   G  PL
Sbjct: 67  IHRSVSSPCFSIAASANMEEDLKATTAPRIEILG-GQRVPTVRALVAEVTMAMVSGAQPL 126

Query: 241 PVHSGLGGAYYFRNNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGF 300
            + SG+GGAY  +  +G ++A+ KP DEEP A NNPK      LG+PG+K S+ VGETG 
Sbjct: 127 LLPSGMGGAYLLQTGKGHNIAVAKPVDEEPLAFNNPKKSGNLMLGQPGMKHSIPVGETGI 186

Query: 301 REVAAYLLDYNHFANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDF 360
           RE+AAYLLDY  F+ VP TALV I+H  F+V+D       S + +  K+AS Q F+ HDF
Sbjct: 187 RELAAYLLDYQGFSGVPPTALVSISHVPFHVSDAF-----SFSSMPYKVASLQRFVGHDF 246

Query: 361 DASDYGTSSFPVVGVHRIGILDIRVFNTDRHGGNLLVRKLD---GVGMFGQVELIPIDHG 420
           DA + G  SF    VHRIGILD+R+ N DRH GN+LV++ D        G  EL+PIDHG
Sbjct: 247 DAGELGPGSFTATSVHRIGILDVRLLNLDRHAGNMLVKRCDKKEAYNRLGTAELVPIDHG 306

Query: 421 LCLPETLEDPYFEWIHWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLI 480
           LCLPE L+DPYFEW++WPQA +PFS+ EL YI +L+PFKD+E+LR EL  + E  +RVL+
Sbjct: 307 LCLPECLDDPYFEWLNWPQALVPFSDTELDYISNLDPFKDAELLRTELHSLPESAIRVLV 366

Query: 481 LCTIFLKEAAAFGLCLAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADD 540
           +CT+FLK+AAA GLCLAEIGE MTR+F  GEE  S LE +C KA+  +  +   + +  D
Sbjct: 367 VCTVFLKQAAAAGLCLAEIGEKMTRDFSKGEESFSLLETLCTKAKASVFGK---TSEDSD 426

Query: 541 LRDEGFQF--DIDCD--EMESGFTQ-EVESDDFFPTTPFPFGIEEENGEEDERGGYLDLF 600
              EG +   ++ C   + + G T  E E  + F  +  P            RG      
Sbjct: 427 YSHEGNEVNTELQCGMFKFDGGDTPCEAEISEVFHVSKPPL---------VPRGP----- 486

Query: 601 TSKRIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASV 660
            +  IP     SMS          +K AK    R G     T  S S   S N      V
Sbjct: 487 RANTIPNDVTASMSSSQNQRITHQEKSAKE-KKRGGKQERCTVRSKSPPISPNHDESKGV 546

Query: 661 TFVKLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQ 698
           +FV   DM    W  FL+ FQ LL  A +K  +         RLG SC+
Sbjct: 547 SFV---DMTTVEWDTFLQSFQTLLQDALSKGST--------PRLGCSCE 560

BLAST of CmaCh12G001180 vs. ExPASy TrEMBL
Match: A0A6J1KIC4 (1-phosphatidylinositol 4-kinase OS=Cucurbita maxima OX=3661 GN=LOC111496041 PE=3 SV=1)

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 629/629 (100.00%), Postives = 629/629 (100.00%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD
Sbjct: 1   MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
           WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC
Sbjct: 61  WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 120

Query: 190 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 249
           LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR
Sbjct: 121 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 180

Query: 250 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 309
           NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF
Sbjct: 181 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 240

Query: 310 ANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 369
           ANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV
Sbjct: 241 ANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 300

Query: 370 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIH 429
           GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 430 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 489
           WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL
Sbjct: 361 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 420

Query: 490 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEME 549
           AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEME
Sbjct: 421 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEME 480

Query: 550 SGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 609
           SGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG
Sbjct: 481 SGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 540

Query: 610 EKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQ 669
           EKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQ
Sbjct: 541 EKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQ 600

Query: 670 ELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           ELLPPAFAKRKSATLGQWQRQRLGTSCQF
Sbjct: 601 ELLPPAFAKRKSATLGQWQRQRLGTSCQF 629

BLAST of CmaCh12G001180 vs. ExPASy TrEMBL
Match: A0A6J1GJQ5 (1-phosphatidylinositol 4-kinase OS=Cucurbita moschata OX=3662 GN=LOC111454481 PE=3 SV=1)

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 615/629 (97.77%), Postives = 619/629 (98.41%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           MHRKLDSPVQTQMAVAAAFTSPLSG EYGGSKRM GRQATGRRRVFVQTDTGCVLGMELD
Sbjct: 1   MHRKLDSPVQTQMAVAAAFTSPLSGGEYGGSKRMEGRQATGRRRVFVQTDTGCVLGMELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
           WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC
Sbjct: 61  WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 120

Query: 190 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 249
           LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDP+PVHSGLGGAYYFR
Sbjct: 121 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPIPVHSGLGGAYYFR 180

Query: 250 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 309
           NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF
Sbjct: 181 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 240

Query: 310 ANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 369
           ANVP TALV+ITHSIFNVNDGVNGNK SKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV
Sbjct: 241 ANVPVTALVRITHSIFNVNDGVNGNKPSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 300

Query: 370 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIH 429
           GVHRIGILDIRVFNTDRHGGNLLVRKLDGVG FGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 430 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 489
           WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL
Sbjct: 361 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 420

Query: 490 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEME 549
           AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDL DEGFQFDIDCDEME
Sbjct: 421 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLTDEGFQFDIDCDEME 480

Query: 550 SGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 609
           SGFTQEVESDDFFPTTPF FGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG
Sbjct: 481 SGFTQEVESDDFFPTTPFRFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 540

Query: 610 EKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQ 669
           EKNKKHAKYFG RP NGYI+TNASSSGRRSANEQ+PASVTFVKLADMNEEVWSLFLEKFQ
Sbjct: 541 EKNKKHAKYFGRRPENGYIMTNASSSGRRSANEQLPASVTFVKLADMNEEVWSLFLEKFQ 600

Query: 670 ELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           ELLPPAFAKRKS TLGQWQRQRLGTSCQF
Sbjct: 601 ELLPPAFAKRKSTTLGQWQRQRLGTSCQF 629

BLAST of CmaCh12G001180 vs. ExPASy TrEMBL
Match: A0A0A0KDZ1 (1-phosphatidylinositol 4-kinase OS=Cucumis sativus OX=3659 GN=Csa_6G401430 PE=3 SV=1)

HSP 1 Score: 1099.7 bits (2843), Expect = 0.0e+00
Identity = 558/648 (86.11%), Postives = 591/648 (91.20%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           MHRKLDSPVQTQMAVAAAF SPLSG EYGGSKRM G+Q TGRRRVFVQTDTGCVLGMELD
Sbjct: 1   MHRKLDSPVQTQMAVAAAFKSPLSG-EYGGSKRMEGKQPTGRRRVFVQTDTGCVLGMELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
            SDNAHTVKRRLQ+ALNVPTDESSLTFGD VLKNDLS VRNDSPLLLTRNILHRSSSTPC
Sbjct: 61  RSDNAHTVKRRLQIALNVPTDESSLTFGDMVLKNDLSAVRNDSPLLLTRNILHRSSSTPC 120

Query: 190 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 249
           LSPTGRDIQQRDQSGPIEILGHS+ FVR KQLV EIIKA KIGVDP+PVHSGLGGAYYFR
Sbjct: 121 LSPTGRDIQQRDQSGPIEILGHSNRFVRTKQLVGEIIKAIKIGVDPIPVHSGLGGAYYFR 180

Query: 250 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 309
           NNRGESVAIVKPTDEEPFAPNNPKG+VGKALG+PGLKRSVRVGETGFREVAAYLLDY+HF
Sbjct: 181 NNRGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKRSVRVGETGFREVAAYLLDYDHF 240

Query: 310 ANVPATALVKITHSIFNVNDGVNGN-KSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPV 369
           ANVP TALVKITHSIFNVNDGVNGN   SK KL+SKIASFQEFIPHDFDASD+GTSSFPV
Sbjct: 241 ANVPHTALVKITHSIFNVNDGVNGNMPPSKKKLISKIASFQEFIPHDFDASDHGTSSFPV 300

Query: 370 VGVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWI 429
           V VHRIGILDIRVFNTDRH GNLLVRKLDGVG FGQVELIPIDHGLCLPETLEDPYFEWI
Sbjct: 301 VAVHRIGILDIRVFNTDRHAGNLLVRKLDGVGRFGQVELIPIDHGLCLPETLEDPYFEWI 360

Query: 430 HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLC 489
           HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIRE CLRVL+LCTIFLKEAAAFGLC
Sbjct: 361 HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREACLRVLVLCTIFLKEAAAFGLC 420

Query: 490 LAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEM 549
           LAEIGEMMTREF SGEEDPSELELIC++ARQLI+E+E+ SP+A DL DE FQF+IDCD  
Sbjct: 421 LAEIGEMMTREFRSGEEDPSELELICMEARQLIEEKEIYSPRA-DLGDEEFQFEIDCDVE 480

Query: 550 ESGFTQEVESDDFFPTTPFPF-----------------GIEEENGEEDER-GGYLDLFTS 609
           ES F+QE+E+DDF+PTTPFPF                  IEEEN EEDER GG+ +L +S
Sbjct: 481 ESDFSQEIEADDFYPTTPFPFSVGSGIHGRFPLRKLEESIEEENDEEDERGGGFTNLLSS 540

Query: 610 KRIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTF 669
           +RIPTISKLSMSLKNT+LGEKNKKH+ YFGTRP NGY++TN +SSG RSANEQ+PASVTF
Sbjct: 541 ERIPTISKLSMSLKNTSLGEKNKKHSNYFGTRPDNGYMMTN-TSSGHRSANEQLPASVTF 600

Query: 670 VKLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           VKLADMNE+ WSLFL+KFQELL PAFAKRKSATLGQ QRQRLGTSCQF
Sbjct: 601 VKLADMNEDSWSLFLDKFQELLHPAFAKRKSATLGQRQRQRLGTSCQF 645

BLAST of CmaCh12G001180 vs. ExPASy TrEMBL
Match: A0A1S3BC69 (1-phosphatidylinositol 4-kinase OS=Cucumis melo OX=3656 GN=LOC103488446 PE=3 SV=1)

HSP 1 Score: 1095.1 bits (2831), Expect = 0.0e+00
Identity = 558/648 (86.11%), Postives = 590/648 (91.05%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           MHRKLDSPVQTQMAVAAAF SPLSG EYGGSKRM G+Q TGRRRVFVQTDTGCVLGMELD
Sbjct: 1   MHRKLDSPVQTQMAVAAAFKSPLSG-EYGGSKRMEGKQPTGRRRVFVQTDTGCVLGMELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
            SDNAHTVKRRLQ+ALNVPTDESSLTFGD VLKNDLS VRNDSPLLLTRNILHRSSSTPC
Sbjct: 61  RSDNAHTVKRRLQIALNVPTDESSLTFGDMVLKNDLSAVRNDSPLLLTRNILHRSSSTPC 120

Query: 190 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 249
           LSPTGRDIQQRDQSGPIEILGHS+ FVR KQLV EIIKA KIGVDP+PVHSGLGGAYYFR
Sbjct: 121 LSPTGRDIQQRDQSGPIEILGHSNRFVRTKQLVGEIIKAIKIGVDPIPVHSGLGGAYYFR 180

Query: 250 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 309
           NNRGESVAIVKPTDEEPFAPNNPKG+VGKALG+PGLKRSVRVGETGFREVAAYLLDY+HF
Sbjct: 181 NNRGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKRSVRVGETGFREVAAYLLDYDHF 240

Query: 310 ANVPATALVKITHSIFNVNDGVNGN-KSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPV 369
           ANVP TALVKITHSIFNVNDGVNGN   SK KLVSKIASFQEFIPHDFDASD+GTSSFPV
Sbjct: 241 ANVPPTALVKITHSIFNVNDGVNGNIPPSKKKLVSKIASFQEFIPHDFDASDHGTSSFPV 300

Query: 370 VGVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWI 429
           V VHRIGILDIRVFNTDRH GNLLVRKLDGVG FGQVELIPIDHGLCLPETLEDPYFEWI
Sbjct: 301 VAVHRIGILDIRVFNTDRHAGNLLVRKLDGVGRFGQVELIPIDHGLCLPETLEDPYFEWI 360

Query: 430 HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLC 489
           HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIRE CLRVL+LCTIFLKEAAAFGLC
Sbjct: 361 HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREACLRVLVLCTIFLKEAAAFGLC 420

Query: 490 LAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEM 549
           LAEIGEMMTREF SGEEDPSELELIC++ARQLI+E+EV SP+A DL DE FQFDIDCD  
Sbjct: 421 LAEIGEMMTREFRSGEEDPSELELICMEARQLIEEKEVYSPRA-DLGDEEFQFDIDCDVD 480

Query: 550 ESGFTQEVESDDFFPTTPFPF-----------------GIEEENGEEDER-GGYLDLFTS 609
           ES F++ +E+DDF+PTTP+ F                  IEEEN EEDER GG+ + F+S
Sbjct: 481 ESDFSEGIEADDFYPTTPYHFSVGSGIHGRFPLRKLEESIEEENDEEDERAGGFTNPFSS 540

Query: 610 KRIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTF 669
           +RIPTISKLSMSLKNT+LGEKNKKH+KYFGTRP NGY++TN +SSG RSANEQ+PASVTF
Sbjct: 541 ERIPTISKLSMSLKNTSLGEKNKKHSKYFGTRPDNGYMMTN-TSSGHRSANEQLPASVTF 600

Query: 670 VKLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           VKLADMNE+ WSLFL+KFQELL PAFAKRKSATLGQ QRQRLGTSCQF
Sbjct: 601 VKLADMNEDSWSLFLDKFQELLHPAFAKRKSATLGQRQRQRLGTSCQF 645

BLAST of CmaCh12G001180 vs. ExPASy TrEMBL
Match: A0A5A7VFX2 (1-phosphatidylinositol 4-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold82G00920 PE=3 SV=1)

HSP 1 Score: 1093.6 bits (2827), Expect = 0.0e+00
Identity = 558/648 (86.11%), Postives = 589/648 (90.90%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           MHRKLDSPVQTQMAVAAAF SPLSG EYGGSKRM G+Q TGRRRVFVQTDTGCVLGMELD
Sbjct: 1   MHRKLDSPVQTQMAVAAAFKSPLSG-EYGGSKRMEGKQPTGRRRVFVQTDTGCVLGMELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
            SDNAHTVKRRLQ+ALNVPTDESSLTFGD VLKNDLS VRNDSPLLLTRNILHRSSSTPC
Sbjct: 61  RSDNAHTVKRRLQIALNVPTDESSLTFGDMVLKNDLSAVRNDSPLLLTRNILHRSSSTPC 120

Query: 190 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 249
           LSPTGRDIQQRDQSGPIEILGHS+ FVR KQLV EIIKA KIGVDP+PVHSGLGGAYYFR
Sbjct: 121 LSPTGRDIQQRDQSGPIEILGHSNRFVRTKQLVGEIIKAIKIGVDPIPVHSGLGGAYYFR 180

Query: 250 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 309
           NNRGESVAIVKPTDEEPFAPNNPKG+VGKALG+PGLKRSVRVGETGFREVAAYLLDY+HF
Sbjct: 181 NNRGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKRSVRVGETGFREVAAYLLDYDHF 240

Query: 310 ANVPATALVKITHSIFNVNDGVNGN-KSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPV 369
           ANVP TALVKITHSIFNVNDGVNGN   SK KLVSKIASFQEFIPHDFDASD+GTSSFPV
Sbjct: 241 ANVPPTALVKITHSIFNVNDGVNGNIPPSKKKLVSKIASFQEFIPHDFDASDHGTSSFPV 300

Query: 370 VGVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWI 429
           V VHRIGILDIRVFNTDRH GNLLVRKLDGVG FGQVELIPIDHGLCLPETLEDPYFEWI
Sbjct: 301 VAVHRIGILDIRVFNTDRHAGNLLVRKLDGVGRFGQVELIPIDHGLCLPETLEDPYFEWI 360

Query: 430 HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLC 489
           HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIRE CLRVL+LCTIFLKEAAAFGLC
Sbjct: 361 HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREACLRVLVLCTIFLKEAAAFGLC 420

Query: 490 LAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEM 549
           LAEIGEMMTREF SGEEDPSELELIC++ARQLI+E+EV SP+A DL DE FQFDIDCD  
Sbjct: 421 LAEIGEMMTREFRSGEEDPSELELICMEARQLIEEKEVYSPRA-DLGDEEFQFDIDCDVD 480

Query: 550 ESGFTQEVESDDFFPTTPFPF-----------------GIEEENGEEDER-GGYLDLFTS 609
           E  F+Q +E+DDF+PTTP+ F                  IEEEN EEDER GG+ + F+S
Sbjct: 481 EWDFSQGIEADDFYPTTPYHFSVGSGIHGRFPLRKLEESIEEENDEEDERAGGFTNPFSS 540

Query: 610 KRIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTF 669
           +RIPTISKLSMSLKNT+LGEKNKKH+KYFGTRP NGY++TN +SSG RSANEQ+PASVTF
Sbjct: 541 ERIPTISKLSMSLKNTSLGEKNKKHSKYFGTRPDNGYMMTN-TSSGHRSANEQLPASVTF 600

Query: 670 VKLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           VKLADMNE+ WSLFL+KFQELL PAFAKRKSATLGQ QRQRLGTSCQF
Sbjct: 601 VKLADMNEDSWSLFLDKFQELLHPAFAKRKSATLGQRQRQRLGTSCQF 645

BLAST of CmaCh12G001180 vs. NCBI nr
Match: XP_023002042.1 (phosphatidylinositol 4-kinase gamma 7-like [Cucurbita maxima])

HSP 1 Score: 1273.8 bits (3295), Expect = 0.0e+00
Identity = 629/629 (100.00%), Postives = 629/629 (100.00%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD
Sbjct: 1   MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
           WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC
Sbjct: 61  WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 120

Query: 190 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 249
           LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR
Sbjct: 121 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 180

Query: 250 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 309
           NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF
Sbjct: 181 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 240

Query: 310 ANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 369
           ANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV
Sbjct: 241 ANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 300

Query: 370 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIH 429
           GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 430 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 489
           WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL
Sbjct: 361 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 420

Query: 490 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEME 549
           AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEME
Sbjct: 421 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEME 480

Query: 550 SGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 609
           SGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG
Sbjct: 481 SGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 540

Query: 610 EKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQ 669
           EKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQ
Sbjct: 541 EKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQ 600

Query: 670 ELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           ELLPPAFAKRKSATLGQWQRQRLGTSCQF
Sbjct: 601 ELLPPAFAKRKSATLGQWQRQRLGTSCQF 629

BLAST of CmaCh12G001180 vs. NCBI nr
Match: XP_022951740.1 (phosphatidylinositol 4-kinase gamma 7-like [Cucurbita moschata])

HSP 1 Score: 1246.5 bits (3224), Expect = 0.0e+00
Identity = 615/629 (97.77%), Postives = 619/629 (98.41%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           MHRKLDSPVQTQMAVAAAFTSPLSG EYGGSKRM GRQATGRRRVFVQTDTGCVLGMELD
Sbjct: 1   MHRKLDSPVQTQMAVAAAFTSPLSGGEYGGSKRMEGRQATGRRRVFVQTDTGCVLGMELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
           WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC
Sbjct: 61  WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 120

Query: 190 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 249
           LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDP+PVHSGLGGAYYFR
Sbjct: 121 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPIPVHSGLGGAYYFR 180

Query: 250 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 309
           NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF
Sbjct: 181 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 240

Query: 310 ANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 369
           ANVP TALV+ITHSIFNVNDGVNGNK SKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV
Sbjct: 241 ANVPVTALVRITHSIFNVNDGVNGNKPSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 300

Query: 370 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIH 429
           GVHRIGILDIRVFNTDRHGGNLLVRKLDGVG FGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 430 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 489
           WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL
Sbjct: 361 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 420

Query: 490 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEME 549
           AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDL DEGFQFDIDCDEME
Sbjct: 421 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLTDEGFQFDIDCDEME 480

Query: 550 SGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 609
           SGFTQEVESDDFFPTTPF FGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG
Sbjct: 481 SGFTQEVESDDFFPTTPFRFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 540

Query: 610 EKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQ 669
           EKNKKHAKYFG RP NGYI+TNASSSGRRSANEQ+PASVTFVKLADMNEEVWSLFLEKFQ
Sbjct: 541 EKNKKHAKYFGRRPENGYIMTNASSSGRRSANEQLPASVTFVKLADMNEEVWSLFLEKFQ 600

Query: 670 ELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           ELLPPAFAKRKS TLGQWQRQRLGTSCQF
Sbjct: 601 ELLPPAFAKRKSTTLGQWQRQRLGTSCQF 629

BLAST of CmaCh12G001180 vs. NCBI nr
Match: KAG7020184.1 (Phosphatidylinositol 4-kinase gamma 7, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1246.1 bits (3223), Expect = 0.0e+00
Identity = 616/629 (97.93%), Postives = 619/629 (98.41%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           MHRKLDSPVQTQMAVAAAFTSPLSG EYGGSKRM GRQATGRRRVFVQTDTGCVLGMELD
Sbjct: 1   MHRKLDSPVQTQMAVAAAFTSPLSGGEYGGSKRMEGRQATGRRRVFVQTDTGCVLGMELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
           WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC
Sbjct: 61  WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 120

Query: 190 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 249
           LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDP+PVHSGLGGAYYFR
Sbjct: 121 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPIPVHSGLGGAYYFR 180

Query: 250 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 309
           NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF
Sbjct: 181 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 240

Query: 310 ANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 369
           ANVPATALVKITHSIFNVNDGVNGNK SKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV
Sbjct: 241 ANVPATALVKITHSIFNVNDGVNGNKPSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 300

Query: 370 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIH 429
           GVHRIGILDIRVFNTDRHGGNLLVRKLDGVG FGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 430 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 489
           WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL
Sbjct: 361 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 420

Query: 490 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEME 549
           AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDL DEGFQFDIDCDEME
Sbjct: 421 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLTDEGFQFDIDCDEME 480

Query: 550 SGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 609
           SGFTQEVESDDFFPTT F FGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG
Sbjct: 481 SGFTQEVESDDFFPTTAFRFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 540

Query: 610 EKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQ 669
           EKNKKHAKYFG RP NGYI+TNASSSGRRSANEQ+PASVTFVKLADMNEEVWSLFLEKFQ
Sbjct: 541 EKNKKHAKYFGRRPENGYIMTNASSSGRRSANEQLPASVTFVKLADMNEEVWSLFLEKFQ 600

Query: 670 ELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           ELLPPAFAKRKS TLGQWQRQRLGTSCQF
Sbjct: 601 ELLPPAFAKRKSTTLGQWQRQRLGTSCQF 629

BLAST of CmaCh12G001180 vs. NCBI nr
Match: XP_023537135.1 (phosphatidylinositol 4-kinase gamma 7-like [Cucurbita pepo subsp. pepo] >XP_023537136.1 phosphatidylinositol 4-kinase gamma 7-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1244.2 bits (3218), Expect = 0.0e+00
Identity = 614/629 (97.62%), Postives = 618/629 (98.25%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           MHRKLDSPVQTQMAVAAAFTSPLSG EYGGSKRM GRQATGRRRVFVQTDTGCVLGMELD
Sbjct: 1   MHRKLDSPVQTQMAVAAAFTSPLSGGEYGGSKRMEGRQATGRRRVFVQTDTGCVLGMELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
           WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC
Sbjct: 61  WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 120

Query: 190 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFR 249
           LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDP+PVHSGLGGAYYFR
Sbjct: 121 LSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPIPVHSGLGGAYYFR 180

Query: 250 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 309
           NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF
Sbjct: 181 NNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHF 240

Query: 310 ANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVV 369
           ANVPATALVKITHSIFNVNDGVNGNK S+ KLVSKIASFQEFIPHDFDASDYGTSSFPVV
Sbjct: 241 ANVPATALVKITHSIFNVNDGVNGNKPSRKKLVSKIASFQEFIPHDFDASDYGTSSFPVV 300

Query: 370 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIH 429
           GVHRIGILDIRVFNTDRHGGNLLVRKLDGVG FGQVELIPIDHGLCLPETLEDPYFEWIH
Sbjct: 301 GVHRIGILDIRVFNTDRHGGNLLVRKLDGVGRFGQVELIPIDHGLCLPETLEDPYFEWIH 360

Query: 430 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 489
           WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL
Sbjct: 361 WPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCL 420

Query: 490 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEME 549
           AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDL DEGFQFDIDCDEME
Sbjct: 421 AEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLSDEGFQFDIDCDEME 480

Query: 550 SGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 609
           S FTQEVESDDFFPTTPF FGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG
Sbjct: 481 SAFTQEVESDDFFPTTPFRFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLG 540

Query: 610 EKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQ 669
           EKNKKHAKYFGTRP NGYI+TNASSSGRRSANEQ+PASVTFVKLADMNEEVWSLFLEKFQ
Sbjct: 541 EKNKKHAKYFGTRPENGYIMTNASSSGRRSANEQLPASVTFVKLADMNEEVWSLFLEKFQ 600

Query: 670 ELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           ELLPPAF KRKS TLGQWQRQRLGTSCQF
Sbjct: 601 ELLPPAFVKRKSTTLGQWQRQRLGTSCQF 629

BLAST of CmaCh12G001180 vs. NCBI nr
Match: KAG6585261.1 (Phosphatidylinositol 4-kinase gamma 7, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1231.5 bits (3185), Expect = 0.0e+00
Identity = 609/622 (97.91%), Postives = 612/622 (98.39%), Query Frame = 0

Query: 77  PVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELDWSDNAHT 136
           PVQTQMAVAAAFTSPLSG EYGGSKRM GRQATGRRRVFVQTDTGCVLGMELDWSDNAHT
Sbjct: 84  PVQTQMAVAAAFTSPLSGGEYGGSKRMEGRQATGRRRVFVQTDTGCVLGMELDWSDNAHT 143

Query: 137 VKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPCLSPTGRD 196
           VKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPCLSPTGRD
Sbjct: 144 VKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPCLSPTGRD 203

Query: 197 IQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFRNNRGESV 256
           IQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDP+PVHSGLGGAYYFRNNRGESV
Sbjct: 204 IQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPIPVHSGLGGAYYFRNNRGESV 263

Query: 257 AIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHFANVPATA 316
           AIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHFANVPATA
Sbjct: 264 AIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHFANVPATA 323

Query: 317 LVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVVGVHRIGI 376
           LVKITHSIFNVNDGVNGNK SKNKLVSKIASFQEFIPHDFDASDYGTSSFPVVGVHRIGI
Sbjct: 324 LVKITHSIFNVNDGVNGNKPSKNKLVSKIASFQEFIPHDFDASDYGTSSFPVVGVHRIGI 383

Query: 377 LDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWIHWPQASIP 436
           LDIRVFNTDRHGGNLLVRKLDGVG FGQVELIPIDHGLCLPETLEDPYFEWIHWPQASIP
Sbjct: 384 LDIRVFNTDRHGGNLLVRKLDGVGRFGQVELIPIDHGLCLPETLEDPYFEWIHWPQASIP 443

Query: 437 FSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCLAEIGEMM 496
           FSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCLAEIGEMM
Sbjct: 444 FSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCLAEIGEMM 503

Query: 497 TREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEMESGFTQEV 556
           TREFHSGEEDPSELELICIKARQLIDEREVLSPQADDL DEGFQFDIDCDEMESGFTQEV
Sbjct: 504 TREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLTDEGFQFDIDCDEMESGFTQEV 563

Query: 557 ESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLGEKNKKHA 616
           ESDDFFPTT F FGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLGEKNKKHA
Sbjct: 564 ESDDFFPTTAFRFGIEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNTNLGEKNKKHA 623

Query: 617 KYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFVKLADMNEEVWSLFLEKFQELLPPAF 676
           KYFG RP NGYI+TNASSSGRRSANEQ+PASVTFVKLADMNEEVWSLFLEKFQELLPPAF
Sbjct: 624 KYFGRRPENGYIMTNASSSGRRSANEQLPASVTFVKLADMNEEVWSLFLEKFQELLPPAF 683

Query: 677 AKRKSATLGQWQRQRLGTSCQF 699
           AKRKS TLGQWQRQRLGTSCQF
Sbjct: 684 AKRKSTTLGQWQRQRLGTSCQF 705

BLAST of CmaCh12G001180 vs. TAIR 10
Match: AT2G03890.1 (phosphoinositide 4-kinase gamma 7 )

HSP 1 Score: 876.7 bits (2264), Expect = 1.2e-254
Identity = 446/660 (67.58%), Postives = 529/660 (80.15%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSK-----RMVGRQATGRRRVFVQTDTGCVL 129
           M R LDSPVQTQMAV A F +PL+G      K     + + RQ++G RRVFVQT+TGCVL
Sbjct: 1   MSRNLDSPVQTQMAV-AVFKTPLTGASKMEGKQHHKHQHLQRQSSG-RRVFVQTETGCVL 60

Query: 130 GMELDWSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRS 189
           GMELD SDN HTVKRRLQ+ALN PT+ESSLT+GD VL NDLS VRNDSPLLL RN +HRS
Sbjct: 61  GMELDRSDNVHTVKRRLQIALNFPTEESSLTYGDMVLTNDLSAVRNDSPLLLKRNFMHRS 120

Query: 190 SSTPCLSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGG 249
           SSTPCLSPTGRD+QQ+D+SGPIEILGHSDCF  +K +V +I+KA K+GV+PLPVHSGLGG
Sbjct: 121 SSTPCLSPTGRDLQQKDRSGPIEILGHSDCFSIVKHMVKDIVKAMKMGVEPLPVHSGLGG 180

Query: 250 AYYFRNNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLL 309
           AYYFRN RGESVAIVKPTDEEPFAPNNPKG+VGKALG+PGLK SVRVGETGFREVAAYLL
Sbjct: 181 AYYFRNKRGESVAIVKPTDEEPFAPNNPKGFVGKALGQPGLKSSVRVGETGFREVAAYLL 240

Query: 310 DYNHFANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTS 369
           DY  FANVP TALVKITHS+FNVNDGV GNK  + KLVSKIASFQ+F+ HDFDASD+GTS
Sbjct: 241 DYGRFANVPPTALVKITHSVFNVNDGVKGNKPREKKLVSKIASFQKFVAHDFDASDHGTS 300

Query: 370 SFPVVGVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPY 429
           SFPV  VHRIGILDIR+FNTDRHGGNLLV+KLDGVGMFGQVELIPIDHGLCLPETLEDPY
Sbjct: 301 SFPVTSVHRIGILDIRIFNTDRHGGNLLVKKLDGVGMFGQVELIPIDHGLCLPETLEDPY 360

Query: 430 FEWIHWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAA 489
           FEWIHWPQAS+PFS++E+ YI+ L+P KD +MLR ELPMIRE CLRVL+LCTIFLKEA+A
Sbjct: 361 FEWIHWPQASLPFSDEEVDYIQSLDPVKDCDMLRRELPMIREACLRVLVLCTIFLKEASA 420

Query: 490 FGLCLAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDID 549
           +GLCLAEIGEMMTREF  GEE+PSELE++CI+A++ + ER+V SP++D + +  FQFD+D
Sbjct: 421 YGLCLAEIGEMMTREFRPGEEEPSELEVVCIEAKRSVTERDVFSPRSDVVGEAEFQFDLD 480

Query: 550 CDEMESGFTQEVE-SDDFFPTTPFPFG--------------IEEENGEEDERGGYLDLFT 609
           CD++ES ++ +++ +DD+F   PF  G               E+E  EED+    + +  
Sbjct: 481 CDDLESVYSSKIQLTDDYFTKNPFSNGRSSLGKLEESIKEEEEDEEEEEDKTENTVPMII 540

Query: 610 SK-----------RIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRR 669
            K           + P++SKLS S+KNT+L +  +K+ K   TR       +  +SSG +
Sbjct: 541 MKDSFFSSAAFHDKAPSLSKLSTSMKNTHLSDTTRKNPKPL-TRG-----KSENTSSGHK 600

Query: 670 SANEQIPASVTFVKLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           SANEQ+P S +FVK+ADM E+ W LFLE+FQELL PAFAKRK+ATL   +RQRLGTSCQF
Sbjct: 601 SANEQLPVSASFVKVADMKEDEWVLFLERFQELLGPAFAKRKTATLS--KRQRLGTSCQF 650

BLAST of CmaCh12G001180 vs. TAIR 10
Match: AT1G13640.1 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 819.3 bits (2115), Expect = 2.3e-237
Identity = 422/638 (66.14%), Postives = 503/638 (78.84%), Query Frame = 0

Query: 86  AAFTSPLSGVEYGGSKRMVGR---------QATGRRRVFVQTDTGCVLGMELDWSDNAHT 145
           A F +PL G E+ G+++M G+         Q+TGRRRVFVQTDTGCVLG+ELD +DN HT
Sbjct: 4   AVFKAPLKG-EFHGARKMEGKQYKHHLLQQQSTGRRRVFVQTDTGCVLGVELDRNDNVHT 63

Query: 146 VKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPCLSPTGRD 205
           VK+RLQ+A N PT+ESSLTFGD VLKNDLS VRNDSPLLL RN++HRSSSTPCLSPTG D
Sbjct: 64  VKKRLQIAFNFPTEESSLTFGDMVLKNDLSAVRNDSPLLLKRNLMHRSSSTPCLSPTGND 123

Query: 206 IQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYFRNNRGESV 265
           +Q++D+SGPIEIL HS CF+ +KQ   +I+KA K+GV+P+PV+ GLGGAYYFR+ +G+SV
Sbjct: 124 LQRKDRSGPIEILSHSPCFLSLKQTANDIVKAMKMGVEPIPVNGGLGGAYYFRDEKGQSV 183

Query: 266 AIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNHFANVPATA 325
           AIVKPTDEEPFAPNNPKG+VGKALG+PGLK SVRVGETGFREVAAYLLDY+HFANVP TA
Sbjct: 184 AIVKPTDEEPFAPNNPKGFVGKALGQPGLKPSVRVGETGFREVAAYLLDYDHFANVPPTA 243

Query: 326 LVKITHSIFNVNDGVNGNKS-SKNKLV-SKIASFQEFIPHDFDASDYGTSSFPVVGVHRI 385
           LVKITHS+FNVNDG++GNKS  K KLV SKIASFQ+F+PHDFDASD+GTSSFPV  VHRI
Sbjct: 244 LVKITHSVFNVNDGMDGNKSREKKKLVSSKIASFQKFVPHDFDASDHGTSSFPVASVHRI 303

Query: 386 GILDIRVFNTDRHGGNLLVRKLD--GVGMFGQVELIPIDHGLCLPETLEDPYFEWIHWPQ 445
           GILDIR+ NTDRHGGNLLV+KLD  GVG FGQVELIPIDHGLCLPETLEDPYFEWIHWPQ
Sbjct: 304 GILDIRILNTDRHGGNLLVKKLDDGGVGRFGQVELIPIDHGLCLPETLEDPYFEWIHWPQ 363

Query: 446 ASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLCLAEI 505
           ASIPFSE+EL YI+ L+P KD EMLR ELPMIRE CLRVL+LCT+FLKEAA FGLCLAEI
Sbjct: 364 ASIPFSEEELDYIQSLDPVKDCEMLRRELPMIREACLRVLVLCTVFLKEAAVFGLCLAEI 423

Query: 506 GEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEMESGF 565
           GEMMTREF +GEE+PSELE++CI+A++L  E++VLSP++D   +  FQFDID +E++S +
Sbjct: 424 GEMMTREFRAGEEEPSELEMLCIEAKRLTTEQDVLSPKSDGEGETEFQFDIDYNELDSVY 483

Query: 566 TQEVESDDFFPTTPFPFG------IEEENGEEDERGGYLDLFTSKRIPTISKLSMSLKNT 625
             E E+D+FF   PF  G      ++E   EE+E     D   +K   ++SKLS S+KN 
Sbjct: 484 GSETETDEFFAKNPFSNGRSSLGELKESIAEEEED----DEEEAKLTLSLSKLSTSMKNN 543

Query: 626 NLGEKNKKHAKYFGTRPGNGYI------VTNASSSGRRSANEQIPASVTFVKLADMNEEV 685
                            G+GY+       T+ +    +SAN Q+P SV FVKLADM E  
Sbjct: 544 ------------LSNTMGSGYLKPPKDNQTDKALVSHKSANVQLPLSVNFVKLADMKEVE 603

Query: 686 WSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           W +FLE+FQELL  AFA+RK+ TL     QRLGTSC+F
Sbjct: 604 WVVFLERFQELLYSAFAERKTMTLR--NTQRLGTSCKF 622

BLAST of CmaCh12G001180 vs. TAIR 10
Match: AT1G26270.1 (Phosphatidylinositol 3- and 4-kinase family protein )

HSP 1 Score: 818.5 bits (2113), Expect = 4.0e-237
Identity = 430/647 (66.46%), Postives = 508/647 (78.52%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSKRMVGRQATGRRRVFVQTDTGCVLGMELD 129
           M RKLDSP++TQMAVA    SPL+G E+    ++  +   GRRRVFVQT+TGCVLG+ELD
Sbjct: 1   MSRKLDSPIKTQMAVALV-KSPLNG-EFREFNKVGMKTPVGRRRVFVQTETGCVLGLELD 60

Query: 130 WSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRSSSTPC 189
            SDNAHTVKR+LQ+ALN P +ESSLTFGD VLKNDL+ VR+DSPLLLTRN  HRSSSTPC
Sbjct: 61  RSDNAHTVKRKLQVALNFPIEESSLTFGDLVLKNDLTAVRSDSPLLLTRNNFHRSSSTPC 120

Query: 190 LSPTGRDIQQ-RDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGGAYYF 249
           LSP   D+QQ RD+S PIEILG+S  F  ++Q+  +I KA K G+DP+ V+SGLGGAYYF
Sbjct: 121 LSPMRADLQQRRDESSPIEILGNSVSFSFVRQMAKDITKAVKKGIDPVAVNSGLGGAYYF 180

Query: 250 RNNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLLDYNH 309
           +N+RGESVAIVKPTDEEP+APNNPKG+VGKALG+PGLKRSVRVGETG+REVAAYLLD  H
Sbjct: 181 KNSRGESVAIVKPTDEEPYAPNNPKGFVGKALGQPGLKRSVRVGETGYREVAAYLLDKEH 240

Query: 310 FANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTSSFPV 369
           FANVP TALVKITHSIFNVNDGV  +K  +  LVSKIAS Q+FIPHD+DAS++GTS+FPV
Sbjct: 241 FANVPPTALVKITHSIFNVNDGVKASKPMEKMLVSKIASLQQFIPHDYDASEHGTSNFPV 300

Query: 370 VGVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPYFEWI 429
             VHRIGILDIR+ NTDRH GNLLV+KLDG GMFGQVEL+PIDHGLCLPETLEDPYFEWI
Sbjct: 301 SAVHRIGILDIRILNTDRHSGNLLVKKLDGDGMFGQVELVPIDHGLCLPETLEDPYFEWI 360

Query: 430 HWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAAFGLC 489
           HWPQASIPFSEDELKYI +L+P  D EMLR ELPM+RE  LRVL+LCTIFLKEAAA GLC
Sbjct: 361 HWPQASIPFSEDELKYIANLDPLGDCEMLRRELPMVREASLRVLVLCTIFLKEAAANGLC 420

Query: 490 LAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDIDCDEM 549
           LAEIGEMMTRE   G+E+PSE+E++C++A  LI E++  SP++D   D  FQFDIDC+E+
Sbjct: 421 LAEIGEMMTREVRPGDEEPSEIEVVCLEAMSLIGEKDAESPRSDLGNDIEFQFDIDCEEV 480

Query: 550 ES-----------------GFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDLFTSK 609
                              G   +VE      TT      EEE+ EE+E     D    +
Sbjct: 481 TDCTKKLALPLGLTFGNARGQLSKVEE-----TTEDGEEEEEEDREEEEN----DRADLE 540

Query: 610 RIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQIPASVTFV 669
           ++PTI KLSMSLK+T LGEK++K+ K+ G R  + Y     +SS  RSA+EQIP+S +FV
Sbjct: 541 KMPTI-KLSMSLKSTLLGEKSQKYQKHPGARVESAY-----ASSAHRSADEQIPSSTSFV 600

Query: 670 KLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           KL+DM+EE W++FLEK+QELL PA  KRKS TLGQ QRQRLGTSCQF
Sbjct: 601 KLSDMSEEEWTIFLEKYQELLYPAIEKRKSITLGQKQRQRLGTSCQF 630

BLAST of CmaCh12G001180 vs. TAIR 10
Match: AT2G03890.2 (phosphoinositide 4-kinase gamma 7 )

HSP 1 Score: 614.4 bits (1583), Expect = 1.1e-175
Identity = 347/660 (52.58%), Postives = 418/660 (63.33%), Query Frame = 0

Query: 70  MHRKLDSPVQTQMAVAAAFTSPLSGVEYGGSK-----RMVGRQATGRRRVFVQTDTGCVL 129
           M R LDSPVQTQMAV A F +PL+G      K     + + RQ++G RRVFVQT+TGCVL
Sbjct: 1   MSRNLDSPVQTQMAV-AVFKTPLTGASKMEGKQHHKHQHLQRQSSG-RRVFVQTETGCVL 60

Query: 130 GMELDWSDNAHTVKRRLQLALNVPTDESSLTFGDTVLKNDLSGVRNDSPLLLTRNILHRS 189
           GMELD SDN HTVKRRLQ+ALN PT+ESSLT+GD VL NDLS                  
Sbjct: 61  GMELDRSDNVHTVKRRLQIALNFPTEESSLTYGDMVLTNDLSS----------------- 120

Query: 190 SSTPCLSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPLPVHSGLGG 249
                                                                       
Sbjct: 121 ------------------------------------------------------------ 180

Query: 250 AYYFRNNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGFREVAAYLL 309
                                                      SVRVGETGFREVAAYLL
Sbjct: 181 -------------------------------------------SVRVGETGFREVAAYLL 240

Query: 310 DYNHFANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDFDASDYGTS 369
           DY  FANVP TALVKITHS+FNVNDGV GNK  + KLVSKIASFQ+F+ HDFDASD+GTS
Sbjct: 241 DYGRFANVPPTALVKITHSVFNVNDGVKGNKPREKKLVSKIASFQKFVAHDFDASDHGTS 300

Query: 370 SFPVVGVHRIGILDIRVFNTDRHGGNLLVRKLDGVGMFGQVELIPIDHGLCLPETLEDPY 429
           SFPV  VHRIGILDIR+FNTDRHGGNLLV+KLDGVGMFGQVELIPIDHGLCLPETLEDPY
Sbjct: 301 SFPVTSVHRIGILDIRIFNTDRHGGNLLVKKLDGVGMFGQVELIPIDHGLCLPETLEDPY 360

Query: 430 FEWIHWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREGCLRVLILCTIFLKEAAA 489
           FEWIHWPQAS+PFS++E+ YI+ L+P KD +MLR ELPMIRE CLRVL+LCTIFLKEA+A
Sbjct: 361 FEWIHWPQASLPFSDEEVDYIQSLDPVKDCDMLRRELPMIREACLRVLVLCTIFLKEASA 420

Query: 490 FGLCLAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVLSPQADDLRDEGFQFDID 549
           +GLCLAEIGEMMTREF  GEE+PSELE++CI+A++ + ER+V SP++D + +  FQFD+D
Sbjct: 421 YGLCLAEIGEMMTREFRPGEEEPSELEVVCIEAKRSVTERDVFSPRSDVVGEAEFQFDLD 480

Query: 550 CDEMESGFTQEVE-SDDFFPTTPFPFG--------------IEEENGEEDERGGYLDLFT 609
           CD++ES ++ +++ +DD+F   PF  G               E+E  EED+    + +  
Sbjct: 481 CDDLESVYSSKIQLTDDYFTKNPFSNGRSSLGKLEESIKEEEEDEEEEEDKTENTVPMII 530

Query: 610 SK-----------RIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRR 669
            K           + P++SKLS S+KNT+L +  +K+ K   TR       +  +SSG +
Sbjct: 541 MKDSFFSSAAFHDKAPSLSKLSTSMKNTHLSDTTRKNPKPL-TRG-----KSENTSSGHK 530

Query: 670 SANEQIPASVTFVKLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSCQF 699
           SANEQ+P S +FVK+ADM E+ W LFLE+FQELL PAFAKRK+ATL   +RQRLGTSCQF
Sbjct: 601 SANEQLPVSASFVKVADMKEDEWVLFLERFQELLGPAFAKRKTATLS--KRQRLGTSCQF 530

BLAST of CmaCh12G001180 vs. TAIR 10
Match: AT3G56600.1 (Protein kinase superfamily protein )

HSP 1 Score: 367.5 bits (942), Expect = 2.4e-101
Identity = 225/542 (41.51%), Postives = 303/542 (55.90%), Query Frame = 0

Query: 177 TRNILHRSSSTPCLSPTGRDIQQRDQSGPIEILGHSDCFVRIKQLVCEIIKATKIGVDPL 236
           + ++ +RS STPC S +G ++     +  IEILG       ++ LV E+  A   G  PL
Sbjct: 51  SHSLNNRSVSTPCFSISGSNL-DGSATPHIEILG-GQRVPTVRSLVAEVTIAIVSGAQPL 110

Query: 237 PVHSGLGGAYYFRNNRGESVAIVKPTDEEPFAPNNPKGYVGKALGEPGLKRSVRVGETGF 296
            + SGLGGAY  +  +G ++A+ KP DEEP A NNPKG  G  LG+PG+KRS+RVGE+G 
Sbjct: 111 LLPSGLGGAYLLQTEKGNNIAVAKPVDEEPLAFNNPKGSGGLTLGQPGMKRSIRVGESGI 170

Query: 297 REVAAYLLDYNHFANVPATALVKITHSIFNVNDGVNGNKSSKNKLVSKIASFQEFIPHDF 356
           RE+AAYLLD+  F++VP TALV+I+H  F        +    +    K+AS Q F+ HDF
Sbjct: 171 RELAAYLLDHQGFSSVPPTALVRISHVPF--------HDRGSDHAAYKVASLQRFVGHDF 230

Query: 357 DASDYGTSSFPVVGVHRIGILDIRVFNTDRHGGNLLVRKL---------DGVGMFGQVEL 416
           DA + G  SF VV VHRIGILD+RV N DRH GN+LV+K+         +GV   G  EL
Sbjct: 231 DAGELGPGSFTVVSVHRIGILDVRVLNLDRHAGNMLVKKIHDQDETTCSNGV---GAAEL 290

Query: 417 IPIDHGLCLPETLEDPYFEWIHWPQASIPFSEDELKYIKDLNPFKDSEMLRMELPMIREG 476
           +PIDHGLCLPE L+DPYFEW++WPQAS+PF++ EL+YI +L+PFKD+E+LR EL  I+E 
Sbjct: 291 VPIDHGLCLPECLDDPYFEWLNWPQASVPFTDIELQYISNLDPFKDAELLRTELDSIQES 350

Query: 477 CLRVLILCTIFLKEAAAFGLCLAEIGEMMTREFHSGEEDPSELELICIKARQLIDEREVL 536
            LRVLI+CTIFLKEAAA GL LAEIGE MTR+   GEE  S LE++C KA+         
Sbjct: 351 SLRVLIVCTIFLKEAAAAGLSLAEIGEKMTRDICRGEESSSVLEILCNKAKASAVSG--- 410

Query: 537 SPQADDLRDEGFQFDIDCDEMESGFTQEVESDDFFPTTPFPFGIEEENGEEDERGGYLDL 596
           S   DD   E  + + + +     F  EVE  +       P                  +
Sbjct: 411 SDDDDDYSSEWNEVEAELECGIFQFDDEVECKELPDMLQVP------------------I 470

Query: 597 FTSKRIPTISKLSMSLKNTNLGEKNKKHAKYFGTRPGNGYIVTNASSSGRRSANEQI--- 656
           FT  R+P+I+    +L                   P N +I T  ++      +  I   
Sbjct: 471 FT--RVPSIAANLSALMRC----------------PPNQWISTYDTNIEEERRDRSIVRS 530

Query: 657 ---PASVTF-----VKLADMNEEVWSLFLEKFQELLPPAFAKRKSATLGQWQRQRLGTSC 699
              P  V +     V   DM+ + W +FL  FQ LLP A       +  +  + R G+SC
Sbjct: 531 KSHPICVNYDEKEGVYFGDMSGDEWEMFLHSFQMLLPEAL----EGSTSKGPKPRFGSSC 536

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SI521.7e-25367.58Phosphatidylinositol 4-kinase gamma 7 OS=Arabidopsis thaliana OX=3702 GN=PI4KG7 ... [more]
Q8W4R83.3e-23666.14Phosphatidylinositol 4-kinase gamma 6 OS=Arabidopsis thaliana OX=3702 GN=PI4KG6 ... [more]
Q9C6715.6e-23666.46Phosphatidylinositol 4-kinase gamma 5 OS=Arabidopsis thaliana OX=3702 GN=PI4KG5 ... [more]
Q0WMZ63.4e-10041.51Phosphatidylinositol 4-kinase gamma 8 OS=Arabidopsis thaliana OX=3702 GN=PI4KG8 ... [more]
O221993.8e-9942.34Phosphatidylinositol 4-kinase gamma 1 OS=Arabidopsis thaliana OX=3702 GN=PI4KG1 ... [more]
Match NameE-valueIdentityDescription
A0A6J1KIC40.0e+00100.001-phosphatidylinositol 4-kinase OS=Cucurbita maxima OX=3661 GN=LOC111496041 PE=3... [more]
A0A6J1GJQ50.0e+0097.771-phosphatidylinositol 4-kinase OS=Cucurbita moschata OX=3662 GN=LOC111454481 PE... [more]
A0A0A0KDZ10.0e+0086.111-phosphatidylinositol 4-kinase OS=Cucumis sativus OX=3659 GN=Csa_6G401430 PE=3 ... [more]
A0A1S3BC690.0e+0086.111-phosphatidylinositol 4-kinase OS=Cucumis melo OX=3656 GN=LOC103488446 PE=3 SV=... [more]
A0A5A7VFX20.0e+0086.111-phosphatidylinositol 4-kinase OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_... [more]
Match NameE-valueIdentityDescription
XP_023002042.10.0e+00100.00phosphatidylinositol 4-kinase gamma 7-like [Cucurbita maxima][more]
XP_022951740.10.0e+0097.77phosphatidylinositol 4-kinase gamma 7-like [Cucurbita moschata][more]
KAG7020184.10.0e+0097.93Phosphatidylinositol 4-kinase gamma 7, partial [Cucurbita argyrosperma subsp. ar... [more]
XP_023537135.10.0e+0097.62phosphatidylinositol 4-kinase gamma 7-like [Cucurbita pepo subsp. pepo] >XP_0235... [more]
KAG6585261.10.0e+0097.91Phosphatidylinositol 4-kinase gamma 7, partial [Cucurbita argyrosperma subsp. so... [more]
Match NameE-valueIdentityDescription
AT2G03890.11.2e-25467.58phosphoinositide 4-kinase gamma 7 [more]
AT1G13640.12.3e-23766.14Phosphatidylinositol 3- and 4-kinase family protein [more]
AT1G26270.14.0e-23766.46Phosphatidylinositol 3- and 4-kinase family protein [more]
AT2G03890.21.1e-17552.58phosphoinositide 4-kinase gamma 7 [more]
AT3G56600.12.4e-10141.51Protein kinase superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000403Phosphatidylinositol 3-/4-kinase, catalytic domainPFAMPF00454PI3_PI4_kinasecoord: 242..492
e-value: 5.5E-44
score: 150.9
NoneNo IPR availablePANTHERPTHR45800:SF18PHOSPHATIDYLINOSITOL 4-KINASE GAMMA 7-LIKE ISOFORM X1coord: 75..698
NoneNo IPR availableCDDcd17039Ubl_ubiquitin_likecoord: 114..176
e-value: 5.59022E-4
score: 36.8054
IPR044571Phosphatidylinositol 4-kinase gamma 1-8PANTHERPTHR45800PHOSPHATIDYLINOSITOL 4-KINASE GAMMAcoord: 75..698
IPR029071Ubiquitin-like domain superfamilySUPERFAMILY54236Ubiquitin-likecoord: 111..176

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G001180.1CmaCh12G001180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046854 phosphatidylinositol phosphate biosynthetic process
biological_process GO:0016310 phosphorylation
molecular_function GO:0004430 1-phosphatidylinositol 4-kinase activity