CmaCh12G000830 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G000830
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAcyl-[acyl-carrier-protein] desaturase
LocationCma_Chr12: 319670 .. 321938 (+)
RNA-Seq ExpressionCmaCh12G000830
SyntenyCmaCh12G000830
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGCGCTCCAATTCCATCTTCTCACCTCTCAATCTCCTAAGTTGCCGTCCTTTGCGATGCCGCAGCTTGCGAGTTTCAGATCTCCTAAGTTCGTTGTGGCCTCCACTCTCCGCTCCACTTCCAGGTAGATTCTCCTTTTTTCTTATTCTGTTTTTCAAATCTGTGTATTGATTTTGATCTCTTTTATTATAATGGAGTGTGTTCCTGAATGGTGATGAATAAGAAGCCGTTTCAATTGCATTGTTATCGTTCTGTTTTTCTTCGTTTTCTATCGCATATCACGTTGACTGAGTGATTATGGCTCATTCCTGCCGGTACGAATAGGTTTCTTCCTTATTCTGTTATAATTCCAGAAAATGAAATATAGATGTTCAATTTTCTTTCAGGTTGCATGATTGTTCATTTGATATTCTTTGGTGTATTGGCGGAGCGGTGTTCACTTTTTCTGTCTTCTTGCGAGTGTTCTATCTTGTTCATTTATTCTTCGCTCTGATCTTCCGCAGCTCATATATTCCTTTCTCCTGACATTTCTCTGCTTATATGTTCATAAACCGTTAATAGACCAAGTTTTTCAGGGTATAATTCTCTGTAATCCGATTCCTTACTTGTTCTACCTTTGGACAGTATTTTTAATTGTAAAACACAGCTCGTTTGCAATGAAGACTTAATTATTTAGAAGAATTGTAATCTGCCTATGCAGGAACGAGAAATCTCTGTTGCTTAACTATTTTGCCTTGTTTTGTGTGTTTCGTAAACATACCAGGGAGGTCGAGGCCCTTAAGAAACCCTTTATACCTCCTAGGGAAGTGCATCCTCAAGTAACTCACTCGATGCCACCACAGAAGATAGAGATGTTTAAATGTCTGGAGGATTGGGCAGCAGAAAACATTTTAGTTCATTTGAAACCAGTGGAGAGGTGTTGGCAACCGCAGGATTTCTTGCCAGATCCGGCATCTGAGGGATTTCAGGAGCAAGTGATGGAGCTTAGAGAGAGGGCAAAGGAAGTCCCTGATGATTACTTCGTCGTTTTAGTTGGAGATATGATTACAGAAGATGCCCTTCCAACTTACCAGACAATGCTTAATACCATGGATGGAGTTAGGGATGAAACGGGAGCAAGCCTCAATTCTTGGGCGATTTGGACGAGGGCTTGGACTGCTGAAGAGAACAGGCATGGTGACCTACTAAACAAATATCTTTACCTATCAGGGCGAGTTGATATGAGGCAGGTTGAGAAAACTATTCAGTATTTGATTGGATCAGGAATGGTGAGATCCATCTAACAAACACCAACGAATTTTCCTTTTCCTCATCTCATTACTTTCAAATTTTGCTTTTCTTCAAACCCACGAGTGATTAAGAGCACAGTATGCACATAAGGCACACGAAAGCGCACACACACACTTGCTGAAGCATTTTGGAGTATTCCACGCATTTTGCTGGATATTGACACAATTTCTCCATTTTTTCACTAGACCTGCTAAATTTTTTATTAAAATTTCATATGGTGTGTTACTATAGGACCCAGGAACTGAAAACAACCCTTACCTAGGATTTATATACACCTCATTTCAAGAGAGAGCAACCTTCATCTCCCATGGGAACACTGCCAGATTAGCAATGGAGAACGGAGACATAAAGTTAGCCCAATTATGTGGGACAATTGCTGCAGATGAGAAGCGACACGAGGTGGCATATACAAAAATCGTTGAAAAGCTGTTTGAGATAGACCCTGAAGGAACCGTCCTGTGTTTCGAAGACATGATGAGGAAGAACATTACAATGCCAGCCCACATGATGTACGATGGCCGTGACAACAACCTCTTCGATCACTTCTCGGCAGTTGCTCAGAGGCTGGGGGTTTATACAGCCAAGGACTACGCCGAAATAGTGGAGTTTTTGGTCGGGAGATGGAAGGTGGAAAGGCTCACAGGACTGTCAGGCGAGGGGCAGAAGGCTCAGGATTATGTGTGTGGGTTACCAGCAAGAATTAGAAGGCTAGAAGAAAGAGCTCAAGCACGGGCGAAAGAAGGTCGTACCATTCCGTTCAGTTGGATTTTCAATCGGGAGGTTAAGCTGTAGTACGTGCAAACAAGCAAATGAATGAATAATCGCCACTGTCGGAGGATAAGAAAATATCAAGTTGTGTTGTTGTGAAAAAATATCGTGTGAGTCAACTAGAGAATGAAGTAGGAGAGTTTTAAGCGTTCTCTACGTTTTTTCTCCTTTTTGTGCAACTTAGCTCAGCTGTAATTTCTGGTTCCTCAG

mRNA sequence

ATGGCGCTCCAATTCCATCTTCTCACCTCTCAATCTCCTAAGTTGCCGTCCTTTGCGATGCCGCAGCTTGCGAGTTTCAGATCTCCTAAGTTCGTTGTGGCCTCCACTCTCCGCTCCACTTCCAGGGAGGTCGAGGCCCTTAAGAAACCCTTTATACCTCCTAGGGAAGTGCATCCTCAAGTAACTCACTCGATGCCACCACAGAAGATAGAGATGTTTAAATGTCTGGAGGATTGGGCAGCAGAAAACATTTTAGTTCATTTGAAACCAGTGGAGAGGTGTTGGCAACCGCAGGATTTCTTGCCAGATCCGGCATCTGAGGGATTTCAGGAGCAAGTGATGGAGCTTAGAGAGAGGGCAAAGGAAGTCCCTGATGATTACTTCGTCGTTTTAGTTGGAGATATGATTACAGAAGATGCCCTTCCAACTTACCAGACAATGCTTAATACCATGGATGGAGTTAGGGATGAAACGGGAGCAAGCCTCAATTCTTGGGCGATTTGGACGAGGGCTTGGACTGCTGAAGAGAACAGGCATGGTGACCTACTAAACAAATATCTTTACCTATCAGGGCGAGTTGATATGAGGCAGGTTGAGAAAACTATTCAGTATTTGATTGGATCAGGAATGGACCCAGGAACTGAAAACAACCCTTACCTAGGATTTATATACACCTCATTTCAAGAGAGAGCAACCTTCATCTCCCATGGGAACACTGCCAGATTAGCAATGGAGAACGGAGACATAAAGTTAGCCCAATTATGTGGGACAATTGCTGCAGATGAGAAGCGACACGAGGTGGCATATACAAAAATCGTTGAAAAGCTGTTTGAGATAGACCCTGAAGGAACCGTCCTGTGTTTCGAAGACATGATGAGGAAGAACATTACAATGCCAGCCCACATGATGTACGATGGCCGTGACAACAACCTCTTCGATCACTTCTCGGCAGTTGCTCAGAGGCTGGGGGTTTATACAGCCAAGGACTACGCCGAAATAGTGGAGTTTTTGGTCGGGAGATGGAAGGTGGAAAGGCTCACAGGACTGTCAGGCGAGGGGCAGAAGGCTCAGGATTATGTGTGTGGGTTACCAGCAAGAATTAGAAGGCTAGAAGAAAGAGCTCAAGCACGGGCGAAAGAAGGTCGTACCATTCCGTTCAGTTGGATTTTCAATCGGGAGGTTAAGCTGTAGTACGTGCAAACAAGCAAATGAATGAATAATCGCCACTGTCGGAGGATAAGAAAATATCAAGTTGTGTTGTTGTGAAAAAATATCGTGTGAGTCAACTAGAGAATGAAGTAGGAGAGTTTTAAGCGTTCTCTACGTTTTTTCTCCTTTTTGTGCAACTTAGCTCAGCTGTAATTTCTGGTTCCTCAG

Coding sequence (CDS)

ATGGCGCTCCAATTCCATCTTCTCACCTCTCAATCTCCTAAGTTGCCGTCCTTTGCGATGCCGCAGCTTGCGAGTTTCAGATCTCCTAAGTTCGTTGTGGCCTCCACTCTCCGCTCCACTTCCAGGGAGGTCGAGGCCCTTAAGAAACCCTTTATACCTCCTAGGGAAGTGCATCCTCAAGTAACTCACTCGATGCCACCACAGAAGATAGAGATGTTTAAATGTCTGGAGGATTGGGCAGCAGAAAACATTTTAGTTCATTTGAAACCAGTGGAGAGGTGTTGGCAACCGCAGGATTTCTTGCCAGATCCGGCATCTGAGGGATTTCAGGAGCAAGTGATGGAGCTTAGAGAGAGGGCAAAGGAAGTCCCTGATGATTACTTCGTCGTTTTAGTTGGAGATATGATTACAGAAGATGCCCTTCCAACTTACCAGACAATGCTTAATACCATGGATGGAGTTAGGGATGAAACGGGAGCAAGCCTCAATTCTTGGGCGATTTGGACGAGGGCTTGGACTGCTGAAGAGAACAGGCATGGTGACCTACTAAACAAATATCTTTACCTATCAGGGCGAGTTGATATGAGGCAGGTTGAGAAAACTATTCAGTATTTGATTGGATCAGGAATGGACCCAGGAACTGAAAACAACCCTTACCTAGGATTTATATACACCTCATTTCAAGAGAGAGCAACCTTCATCTCCCATGGGAACACTGCCAGATTAGCAATGGAGAACGGAGACATAAAGTTAGCCCAATTATGTGGGACAATTGCTGCAGATGAGAAGCGACACGAGGTGGCATATACAAAAATCGTTGAAAAGCTGTTTGAGATAGACCCTGAAGGAACCGTCCTGTGTTTCGAAGACATGATGAGGAAGAACATTACAATGCCAGCCCACATGATGTACGATGGCCGTGACAACAACCTCTTCGATCACTTCTCGGCAGTTGCTCAGAGGCTGGGGGTTTATACAGCCAAGGACTACGCCGAAATAGTGGAGTTTTTGGTCGGGAGATGGAAGGTGGAAAGGCTCACAGGACTGTCAGGCGAGGGGCAGAAGGCTCAGGATTATGTGTGTGGGTTACCAGCAAGAATTAGAAGGCTAGAAGAAAGAGCTCAAGCACGGGCGAAAGAAGGTCGTACCATTCCGTTCAGTTGGATTTTCAATCGGGAGGTTAAGCTGTAG

Protein sequence

MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERAKEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPAHMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYVCGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL
Homology
BLAST of CmaCh12G000830 vs. ExPASy Swiss-Prot
Match: P32061 (Acyl-[acyl-carrier-protein] desaturase, chloroplastic OS=Cucumis sativus OX=3659 PE=2 SV=1)

HSP 1 Score: 720.3 bits (1858), Expect = 1.2e-206
Identity = 348/396 (87.88%), Postives = 371/396 (93.69%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MAL+FH LTSQSPKLPSF MPQLAS RSPKFV+ASTLRSTSREVE LKKPF+PPREVH Q
Sbjct: 1   MALKFHPLTSQSPKLPSFRMPQLASLRSPKFVMASTLRSTSREVETLKKPFMPPREVHLQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQK+E+FK LEDWA EN+LVHLKPVERCWQPQDFLPD A EGF EQV ELRERA
Sbjct: 61  VTHSMPPQKMEIFKSLEDWAEENLLVHLKPVERCWQPQDFLPDSAFEGFHEQVRELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KE+PD+YFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGAS   WAIWTRAWTAEENRHG
Sbjct: 121 KELPDEYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTPWAIWTRAWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDP TENNPYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLA E+GDIKLAQ+CGTI ADEKRHE AYTKIVEKLFEIDPEGTV+ FE+MMRK ++MPA
Sbjct: 241 RLAKEHGDIKLAQICGTITADEKRHETAYTKIVEKLFEIDPEGTVIAFEEMMRKKVSMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           H+MYDGRD+NLF HFSAVAQRLGVYTAKDYA+I+EFLVGRWKVE LTGLSGEGQKAQDYV
Sbjct: 301 HLMYDGRDDNLFHHFSAVAQRLGVYTAKDYADILEFLVGRWKVESLTGLSGEGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           C LPARIR+LEERAQ RAKEG TIPFSWIF+R+VKL
Sbjct: 361 CALPARIRKLEERAQGRAKEGPTIPFSWIFDRQVKL 396

BLAST of CmaCh12G000830 vs. ExPASy Swiss-Prot
Match: P22337 (Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Ricinus communis OX=3988 PE=1 SV=1)

HSP 1 Score: 694.5 bits (1791), Expect = 6.9e-199
Identity = 334/396 (84.34%), Postives = 366/396 (92.42%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MAL+ +   SQ+ KLPSFA+P +AS RSPKF +ASTL+S S+EVE LKKPF+PPREVH Q
Sbjct: 1   MALKLNPFLSQTQKLPSFALPPMASTRSPKFYMASTLKSGSKEVENLKKPFMPPREVHVQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIE+FK L++WA ENILVHLKPVE+CWQPQDFLPDPAS+GF EQV ELRERA
Sbjct: 61  VTHSMPPQKIEIFKSLDNWAEENILVHLKPVEKCWQPQDFLPDPASDGFDEQVRELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KE+PDDYFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGAS  SWAIWTRAWTAEENRHG
Sbjct: 121 KEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDMRQ+EKTIQYLIGSGMDP TEN+PYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           R A E+GDIKLAQ+CGTIAADEKRHE AYTKIVEKLFEIDP+GTVL F DMMRK I+MPA
Sbjct: 241 RQAKEHGDIKLAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVLAFADMMRKKISMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           H+MYDGRD+NLFDHFSAVAQRLGVYTAKDYA+I+EFLVGRWKV++LTGLS EGQKAQDYV
Sbjct: 301 HLMYDGRDDNLFDHFSAVAQRLGVYTAKDYADILEFLVGRWKVDKLTGLSAEGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           C LP RIRRLEERAQ RAKE  T+PFSWIF+R+VKL
Sbjct: 361 CRLPPRIRRLEERAQGRAKEAPTMPFSWIFDRQVKL 396

BLAST of CmaCh12G000830 vs. ExPASy Swiss-Prot
Match: Q42807 (Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Glycine max OX=3847 GN=ACPD PE=2 SV=1)

HSP 1 Score: 664.5 bits (1713), Expect = 7.7e-190
Identity = 316/393 (80.41%), Postives = 359/393 (91.35%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MAL+ + + +Q     +F++PQ+AS RSP+F +ASTLRS S+EVE +KKPF PPREVH Q
Sbjct: 1   MALRLNPIPTQ-----TFSLPQMASLRSPRFRMASTLRSGSKEVENIKKPFTPPREVHVQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIE+F+ LEDWA ENIL HLKPVE+CWQPQDFLPDP+S+GF+EQV ELRERA
Sbjct: 61  VTHSMPPQKIEIFQSLEDWAEENILAHLKPVEKCWQPQDFLPDPSSDGFEEQVKELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KE+PDDYFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGASL SWAIWTRAWTAEENRHG
Sbjct: 121 KELPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASLTSWAIWTRAWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDM+Q+EKTIQYLIGSGMDP TEN+PYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMKQIEKTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLA E+GDIKLAQ+CG IA+DEKRHE AYTKIVEKLFE DP+GTV+ F DMMRK I MPA
Sbjct: 241 RLAKEHGDIKLAQICGMIASDEKRHETAYTKIVEKLFESDPDGTVMAFADMMRKKIAMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           H+MYDGRD+NLFD++S+VAQR+GVYTAKDYA+I+EFLVGRWKVE+LTGLSGEG+KAQ+Y+
Sbjct: 301 HLMYDGRDDNLFDNYSSVAQRIGVYTAKDYADILEFLVGRWKVEQLTGLSGEGRKAQEYI 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNRE 394
           CGLP RIRRLEERAQAR KE  T+ FSWI +RE
Sbjct: 361 CGLPPRIRRLEERAQARVKESSTLKFSWIHDRE 388

BLAST of CmaCh12G000830 vs. ExPASy Swiss-Prot
Match: P22243 (Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Carthamus tinctorius OX=4222 PE=1 SV=1)

HSP 1 Score: 662.9 bits (1709), Expect = 2.2e-189
Identity = 316/396 (79.80%), Postives = 356/396 (89.90%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MAL+   +T QS +  SF+ P+ A+ RSPKF +ASTL S++ +V+  KKPF PPREVH Q
Sbjct: 1   MALRITPVTLQSERYRSFSFPKKANLRSPKFAMASTLGSSTPKVDNAKKPFQPPREVHVQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIE+FK +E WA +NILVHLKPVE+CWQ QDFLPDPASEGF EQV ELR RA
Sbjct: 61  VTHSMPPQKIEIFKSIEGWAEQNILVHLKPVEKCWQAQDFLPDPASEGFDEQVKELRARA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KE+PDDYFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGASL  WA+WTRAWTAEENRHG
Sbjct: 121 KEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASLTPWAVWTRAWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLL+ YLYLSGRVDMRQ++KTIQYLIGSGMDP TEN+PYLGFIYTSFQERATF+SHGNTA
Sbjct: 181 DLLHTYLYLSGRVDMRQIQKTIQYLIGSGMDPRTENSPYLGFIYTSFQERATFVSHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           R A ++GD+KLAQ+CGTIA+DEKRHE AYTKIVEKLFEIDP+GTVL F DMMRK I+MPA
Sbjct: 241 RHAKDHGDVKLAQICGTIASDEKRHETAYTKIVEKLFEIDPDGTVLAFADMMRKKISMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           H+MYDGRD+NLF+HFSAVAQRLGVYTAKDYA+I+EFLVGRWKV  LTGLSGEG+KAQDYV
Sbjct: 301 HLMYDGRDDNLFEHFSAVAQRLGVYTAKDYADILEFLVGRWKVADLTGLSGEGRKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLP RIRRLEERAQ RAKEG  +PFSWIF+R+VKL
Sbjct: 361 CGLPPRIRRLEERAQGRAKEGPVVPFSWIFDRQVKL 396

BLAST of CmaCh12G000830 vs. ExPASy Swiss-Prot
Match: O22832 (Stearoyl-[acyl-carrier-protein] 9-desaturase 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=FAB2 PE=1 SV=1)

HSP 1 Score: 649.8 bits (1675), Expect = 2.0e-185
Identity = 315/401 (78.55%), Postives = 357/401 (89.03%), Query Frame = 0

Query: 1   MALQFH-LLTSQSPKLPSFAMPQLASFRSPKFVVAST----LRSTSREVEALKKPFIPPR 60
           MAL+F+ L+ SQ  K PS   P   SFRSPKF+  ++    L S  +EVE+LKKPF PPR
Sbjct: 1   MALKFNPLVASQPYKFPSSTRPPTPSFRSPKFLCLASSSPALSSGPKEVESLKKPFTPPR 60

Query: 61  EVHPQVTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVME 120
           EVH QV HSMPPQKIE+FK +E+WA EN+L+HLK VE+ WQPQDFLPDPAS+GF++QV E
Sbjct: 61  EVHVQVLHSMPPQKIEIFKSMENWAEENLLIHLKDVEKSWQPQDFLPDPASDGFEDQVRE 120

Query: 121 LRERAKEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAE 180
           LRERA+E+PDDYFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGAS  SWAIWTRAWTAE
Sbjct: 121 LRERARELPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAE 180

Query: 181 ENRHGDLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFIS 240
           ENRHGDLLNKYLYLSGRVDMRQ+EKTIQYLIGSGMDP TENNPYLGFIYTSFQERATFIS
Sbjct: 181 ENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFIS 240

Query: 241 HGNTARLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKN 300
           HGNTAR A E+GDIKLAQ+CGTIAADEKRHE AYTKIVEKLFEIDP+GTV+ F DMMRK 
Sbjct: 241 HGNTARQAKEHGDIKLAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVMAFADMMRKK 300

Query: 301 ITMPAHMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQK 360
           I+MPAH+MYDGR++NLFD+FS+VAQRLGVYTAKDYA+I+EFLVGRWK++ LTGLSGEG K
Sbjct: 301 ISMPAHLMYDGRNDNLFDNFSSVAQRLGVYTAKDYADILEFLVGRWKIQDLTGLSGEGNK 360

Query: 361 AQDYVCGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           AQDY+CGL  RI+RL+ERAQARAK+G  IPFSWI +REV+L
Sbjct: 361 AQDYLCGLAPRIKRLDERAQARAKKGPKIPFSWIHDREVQL 401

BLAST of CmaCh12G000830 vs. ExPASy TrEMBL
Match: A0A6J1KQ22 (Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita maxima OX=3661 GN=LOC111496572 PE=3 SV=1)

HSP 1 Score: 810.1 bits (2091), Expect = 4.2e-231
Identity = 396/396 (100.00%), Postives = 396/396 (100.00%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ
Sbjct: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA
Sbjct: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG
Sbjct: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA
Sbjct: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV
Sbjct: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL
Sbjct: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 396

BLAST of CmaCh12G000830 vs. ExPASy TrEMBL
Match: A0A6J1GHE1 (Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC111453985 PE=3 SV=1)

HSP 1 Score: 770.4 bits (1988), Expect = 3.7e-219
Identity = 372/396 (93.94%), Postives = 387/396 (97.73%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ
Sbjct: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIEMFKCLEDWAAENILV+LKPVERCWQP D LPDPASEGFQEQVMELRERA
Sbjct: 61  VTHSMPPQKIEMFKCLEDWAAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KEVPDDYF+VLVGDMITEDALPTYQTMLNTMDGVRDETGASL+SWAIWTR+WTAEENRHG
Sbjct: 121 KEVPDDYFIVLVGDMITEDALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           D+LNKYLYLSGRVDMRQVEKTIQYLI SGMDPGTENNPYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DVLNKYLYLSGRVDMRQVEKTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLA E+GDIKLAQLCGTIAADEKRHE AYTKIVEKLFEIDPEGT+LCFEDMMRKNITMPA
Sbjct: 241 RLAKEHGDIKLAQLCGTIAADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           HMMYDGRDNNLF+HFSA+AQRLGVYTAKDYA+I+E+LVGRWKVE+L GLSG GQKAQDYV
Sbjct: 301 HMMYDGRDNNLFNHFSALAQRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLPARIRRLEERA+ RAKEG T+PFSWIFNREVKL
Sbjct: 361 CGLPARIRRLEERARTRAKEGPTVPFSWIFNREVKL 396

BLAST of CmaCh12G000830 vs. ExPASy TrEMBL
Match: A0A6J1BQY3 (Acyl-[acyl-carrier-protein] desaturase OS=Momordica charantia OX=3673 GN=LOC111004796 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 6.9e-210
Identity = 355/396 (89.65%), Postives = 378/396 (95.45%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MAL+FH LTSQSPKLPSFA+PQLAS RSPKF++ASTLRSTSREVE LKKPF+PPREVH Q
Sbjct: 1   MALKFHPLTSQSPKLPSFALPQLASLRSPKFLMASTLRSTSREVETLKKPFMPPREVHLQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIEMFK +E+WA ENILVHLKPVERCWQPQDFLPDPAS+GF EQVMELRERA
Sbjct: 61  VTHSMPPQKIEMFKSMENWAEENILVHLKPVERCWQPQDFLPDPASDGFHEQVMELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KE+PDDYFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGASL SWAIWTRAWTAEENRHG
Sbjct: 121 KEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASLTSWAIWTRAWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDMRQ+EKTIQYLIGSGMDP TENNPYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           R A ++GDIKLAQ+CGTIAADEKRHE AYTKIVEKLFEIDPEGTV+ FEDMMRK ++MPA
Sbjct: 241 RQAKQHGDIKLAQICGTIAADEKRHETAYTKIVEKLFEIDPEGTVIAFEDMMRKKVSMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           H+MYDGRD+NLFDHFSAVAQRLGVYTAKDYA+I+EFLVGRWKVE+LTGLS EGQKAQDYV
Sbjct: 301 HLMYDGRDDNLFDHFSAVAQRLGVYTAKDYADILEFLVGRWKVEKLTGLSAEGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLPARIRRLEERAQ RAKE  TIPFSWIFNREVKL
Sbjct: 361 CGLPARIRRLEERAQGRAKEAATIPFSWIFNREVKL 396

BLAST of CmaCh12G000830 vs. ExPASy TrEMBL
Match: A0A1S3BAE5 (Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo OX=3656 GN=LOC103487766 PE=3 SV=1)

HSP 1 Score: 739.6 bits (1908), Expect = 6.9e-210
Identity = 355/396 (89.65%), Postives = 379/396 (95.71%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MAL+FH LTSQSPKLPSFAMPQLAS RSPKFV+ASTLRSTSREVE LKKPF+PPREVH Q
Sbjct: 1   MALKFHPLTSQSPKLPSFAMPQLASLRSPKFVMASTLRSTSREVETLKKPFMPPREVHLQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIE+FK LEDWA EN+LVHLKPVERCWQPQDFLPDPA +GF EQVMELRERA
Sbjct: 61  VTHSMPPQKIEIFKSLEDWAEENLLVHLKPVERCWQPQDFLPDPAFDGFHEQVMELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KE+PDDYFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGAS  SWAIWTRAWTAEENRHG
Sbjct: 121 KEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDMRQ+EKTIQYLIGSGMDP TENNPYLGFIYTSFQERATF+SHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFVSHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLA E+GDIKLAQ+CGTIAADEKRHE AYTKIVEKLFEIDPEGTV+ FEDMMRK ++MPA
Sbjct: 241 RLAKEHGDIKLAQICGTIAADEKRHETAYTKIVEKLFEIDPEGTVIAFEDMMRKKVSMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           H+MYDGRD+NLFDHFSAVAQRLGVYTAKDYA+I+EFLVGRWKVE LTGLSGEGQKAQDYV
Sbjct: 301 HLMYDGRDDNLFDHFSAVAQRLGVYTAKDYADILEFLVGRWKVESLTGLSGEGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLPARIR+LEERAQ+RAKEG TIPFSWIF+R+VKL
Sbjct: 361 CGLPARIRKLEERAQSRAKEGPTIPFSWIFDRQVKL 396

BLAST of CmaCh12G000830 vs. ExPASy TrEMBL
Match: A0A6J1HDY5 (Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC111462685 PE=3 SV=1)

HSP 1 Score: 733.4 bits (1892), Expect = 5.0e-208
Identity = 354/396 (89.39%), Postives = 374/396 (94.44%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MAL+FH L  QSPK PSFAMPQLAS RSPKFV+ASTL STSREVE LKKPF+PPREVH Q
Sbjct: 1   MALKFHPLACQSPKFPSFAMPQLASLRSPKFVMASTLHSTSREVETLKKPFMPPREVHLQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIE+FK LEDWA ENIL HLKPVERCWQPQDFLPDPASEGF EQVMELRERA
Sbjct: 61  VTHSMPPQKIEIFKSLEDWAEENILCHLKPVERCWQPQDFLPDPASEGFHEQVMELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KE+PDDYFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGAS  SWAIWTRAWTAEENRHG
Sbjct: 121 KELPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDMRQ+EKTIQYLIGSGMDP TENNPYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLA ++GDIKLAQ+CGTIAADEKRHE AYTKIVEKLFEIDPEGTV+ FEDMMRK ++MPA
Sbjct: 241 RLAKDHGDIKLAQICGTIAADEKRHETAYTKIVEKLFEIDPEGTVIAFEDMMRKKVSMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           H+MYDGRD+NLFDHFSAVAQRLGVYTAKDYA+I+EFLV RWKVE+LTGLSGEGQKAQDYV
Sbjct: 301 HLMYDGRDDNLFDHFSAVAQRLGVYTAKDYADILEFLVARWKVEKLTGLSGEGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLPARIRRLEERAQARAKEG  IPFSWIF+REVKL
Sbjct: 361 CGLPARIRRLEERAQARAKEGPAIPFSWIFDREVKL 396

BLAST of CmaCh12G000830 vs. NCBI nr
Match: XP_023002825.1 (acyl-[acyl-carrier-protein] desaturase, chloroplastic-like [Cucurbita maxima])

HSP 1 Score: 810.1 bits (2091), Expect = 8.6e-231
Identity = 396/396 (100.00%), Postives = 396/396 (100.00%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ
Sbjct: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA
Sbjct: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG
Sbjct: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA
Sbjct: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV
Sbjct: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL
Sbjct: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 396

BLAST of CmaCh12G000830 vs. NCBI nr
Match: KAG6585228.1 (hypothetical protein SDJN03_17961, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 777.7 bits (2007), Expect = 4.8e-221
Identity = 377/396 (95.20%), Postives = 388/396 (97.98%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ
Sbjct: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIEMFKCLEDWAAENILV+LKPVERCWQP D LPDPASEGFQEQVMELRERA
Sbjct: 61  VTHSMPPQKIEMFKCLEDWAAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KEVPDDYF+VLVGDMITEDALPTYQTMLNTMDGVRDETGASL+SWAIWTR+WTAEENRHG
Sbjct: 121 KEVPDDYFIVLVGDMITEDALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLA E+GDIKLAQLCGTIAADEKRHE AYTKIVEKLFEIDPEGT+LCFEDMMRKNITMPA
Sbjct: 241 RLAKEHGDIKLAQLCGTIAADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           HMMYDGRDNNLF+HFSAVAQRLGVYTAKDYA+IVE+LVGRWKVE+LTGLSG GQKAQDYV
Sbjct: 301 HMMYDGRDNNLFNHFSAVAQRLGVYTAKDYADIVEYLVGRWKVEQLTGLSGHGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLPARIRRLEERAQ RAKEG T+ FSWIFNREVKL
Sbjct: 361 CGLPARIRRLEERAQTRAKEGPTVAFSWIFNREVKL 396

BLAST of CmaCh12G000830 vs. NCBI nr
Match: XP_023536832.1 (acyl-[acyl-carrier-protein] desaturase, chloroplastic-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 773.9 bits (1997), Expect = 6.9e-220
Identity = 377/396 (95.20%), Postives = 387/396 (97.73%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MALQFHLL+SQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPF PPREVH Q
Sbjct: 1   MALQFHLLSSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFKPPREVHLQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPP KIEMFKCLEDWAAENILV+LKPVERCWQP DFLPDPASEGFQE VMELRERA
Sbjct: 61  VTHSMPPHKIEMFKCLEDWAAENILVYLKPVERCWQPLDFLPDPASEGFQEHVMELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASL+SWAIWTR+WTAEENRHG
Sbjct: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLA E+GDIKLAQLCGTIAADEKRHE AYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA
Sbjct: 241 RLAKEHGDIKLAQLCGTIAADEKRHEAAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           HMMYDGRDNNLF+HFSAVAQRLGVYTAKDYA+IVEFLVGRWKVE+LTGLSG+GQKAQDYV
Sbjct: 301 HMMYDGRDNNLFNHFSAVAQRLGVYTAKDYADIVEFLVGRWKVEQLTGLSGQGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLPARIRRLEERAQ RAKEG T+PFSWIFNREVKL
Sbjct: 361 CGLPARIRRLEERAQTRAKEGPTVPFSWIFNREVKL 396

BLAST of CmaCh12G000830 vs. NCBI nr
Match: XP_022951010.1 (acyl-[acyl-carrier-protein] desaturase, chloroplastic-like [Cucurbita moschata])

HSP 1 Score: 770.4 bits (1988), Expect = 7.6e-219
Identity = 372/396 (93.94%), Postives = 387/396 (97.73%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ
Sbjct: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIEMFKCLEDWAAENILV+LKPVERCWQP D LPDPASEGFQEQVMELRERA
Sbjct: 61  VTHSMPPQKIEMFKCLEDWAAENILVYLKPVERCWQPLDLLPDPASEGFQEQVMELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KEVPDDYF+VLVGDMITEDALPTYQTMLNTMDGVRDETGASL+SWAIWTR+WTAEENRHG
Sbjct: 121 KEVPDDYFIVLVGDMITEDALPTYQTMLNTMDGVRDETGASLSSWAIWTRSWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           D+LNKYLYLSGRVDMRQVEKTIQYLI SGMDPGTENNPYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DVLNKYLYLSGRVDMRQVEKTIQYLIRSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLA E+GDIKLAQLCGTIAADEKRHE AYTKIVEKLFEIDPEGT+LCFEDMMRKNITMPA
Sbjct: 241 RLAKEHGDIKLAQLCGTIAADEKRHEAAYTKIVEKLFEIDPEGTILCFEDMMRKNITMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           HMMYDGRDNNLF+HFSA+AQRLGVYTAKDYA+I+E+LVGRWKVE+L GLSG GQKAQDYV
Sbjct: 301 HMMYDGRDNNLFNHFSALAQRLGVYTAKDYADILEYLVGRWKVEQLKGLSGHGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLPARIRRLEERA+ RAKEG T+PFSWIFNREVKL
Sbjct: 361 CGLPARIRRLEERARTRAKEGPTVPFSWIFNREVKL 396

BLAST of CmaCh12G000830 vs. NCBI nr
Match: XP_038885157.1 (acyl-[acyl-carrier-protein] desaturase, chloroplastic [Benincasa hispida] >XP_038885158.1 acyl-[acyl-carrier-protein] desaturase, chloroplastic [Benincasa hispida])

HSP 1 Score: 740.3 bits (1910), Expect = 8.4e-210
Identity = 357/396 (90.15%), Postives = 378/396 (95.45%), Query Frame = 0

Query: 1   MALQFHLLTSQSPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQ 60
           MAL+FH LTSQSPKLPSFAMPQLAS RSPKFV+ASTLRSTSREVE LKKPF+PPREVH Q
Sbjct: 1   MALKFHPLTSQSPKLPSFAMPQLASLRSPKFVMASTLRSTSREVETLKKPFMPPREVHLQ 60

Query: 61  VTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERA 120
           VTHSMPPQKIE+FK LEDWA ENILVHLKPVERCWQPQDFLPDPASEGF EQVMELRERA
Sbjct: 61  VTHSMPPQKIEIFKSLEDWAEENILVHLKPVERCWQPQDFLPDPASEGFHEQVMELRERA 120

Query: 121 KEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHG 180
           KE+PDDYFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGAS  SWAIWTRAWTAEENRHG
Sbjct: 121 KEIPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAEENRHG 180

Query: 181 DLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTA 240
           DLLNKYLYLSGRVDMRQ+EKTIQYLIGSGMDP TENNPYLGFIYTSFQERATFISHGNTA
Sbjct: 181 DLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFISHGNTA 240

Query: 241 RLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPA 300
           RLA E+GDIKLAQ+CGTIAADEKRHE AYTKIVEKLFEIDPEGTV+ FEDMMRK ++MPA
Sbjct: 241 RLAKEHGDIKLAQICGTIAADEKRHETAYTKIVEKLFEIDPEGTVIAFEDMMRKKVSMPA 300

Query: 301 HMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYV 360
           H+MYDG D++LFDHFSAVAQRLGVYTAKDYA+I+EFLVGRW+VE LTGLSGEGQKAQDYV
Sbjct: 301 HLMYDGHDDSLFDHFSAVAQRLGVYTAKDYADILEFLVGRWRVENLTGLSGEGQKAQDYV 360

Query: 361 CGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           CGLPARIR+LEERAQ RAKEG TIPFSWIF+REVKL
Sbjct: 361 CGLPARIRKLEERAQGRAKEGPTIPFSWIFDREVKL 396

BLAST of CmaCh12G000830 vs. TAIR 10
Match: AT2G43710.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 649.8 bits (1675), Expect = 1.4e-186
Identity = 315/401 (78.55%), Postives = 357/401 (89.03%), Query Frame = 0

Query: 1   MALQFH-LLTSQSPKLPSFAMPQLASFRSPKFVVAST----LRSTSREVEALKKPFIPPR 60
           MAL+F+ L+ SQ  K PS   P   SFRSPKF+  ++    L S  +EVE+LKKPF PPR
Sbjct: 1   MALKFNPLVASQPYKFPSSTRPPTPSFRSPKFLCLASSSPALSSGPKEVESLKKPFTPPR 60

Query: 61  EVHPQVTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVME 120
           EVH QV HSMPPQKIE+FK +E+WA EN+L+HLK VE+ WQPQDFLPDPAS+GF++QV E
Sbjct: 61  EVHVQVLHSMPPQKIEIFKSMENWAEENLLIHLKDVEKSWQPQDFLPDPASDGFEDQVRE 120

Query: 121 LRERAKEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAE 180
           LRERA+E+PDDYFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGAS  SWAIWTRAWTAE
Sbjct: 121 LRERARELPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAE 180

Query: 181 ENRHGDLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFIS 240
           ENRHGDLLNKYLYLSGRVDMRQ+EKTIQYLIGSGMDP TENNPYLGFIYTSFQERATFIS
Sbjct: 181 ENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFIS 240

Query: 241 HGNTARLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKN 300
           HGNTAR A E+GDIKLAQ+CGTIAADEKRHE AYTKIVEKLFEIDP+GTV+ F DMMRK 
Sbjct: 241 HGNTARQAKEHGDIKLAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVMAFADMMRKK 300

Query: 301 ITMPAHMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQK 360
           I+MPAH+MYDGR++NLFD+FS+VAQRLGVYTAKDYA+I+EFLVGRWK++ LTGLSGEG K
Sbjct: 301 ISMPAHLMYDGRNDNLFDNFSSVAQRLGVYTAKDYADILEFLVGRWKIQDLTGLSGEGNK 360

Query: 361 AQDYVCGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           AQDY+CGL  RI+RL+ERAQARAK+G  IPFSWI +REV+L
Sbjct: 361 AQDYLCGLAPRIKRLDERAQARAKKGPKIPFSWIHDREVQL 401

BLAST of CmaCh12G000830 vs. TAIR 10
Match: AT2G43710.2 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 647.9 bits (1670), Expect = 5.3e-186
Identity = 314/401 (78.30%), Postives = 356/401 (88.78%), Query Frame = 0

Query: 1   MALQFH-LLTSQSPKLPSFAMPQLASFRSPKFVVAST----LRSTSREVEALKKPFIPPR 60
           MAL+F+ L+ SQ  K PS   P   SFRSPKF+  ++    L S  + VE+LKKPF PPR
Sbjct: 1   MALKFNPLVASQPYKFPSSTRPPTPSFRSPKFLCLASSSPALSSGPKSVESLKKPFTPPR 60

Query: 61  EVHPQVTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVME 120
           EVH QV HSMPPQKIE+FK +E+WA EN+L+HLK VE+ WQPQDFLPDPAS+GF++QV E
Sbjct: 61  EVHVQVLHSMPPQKIEIFKSMENWAEENLLIHLKDVEKSWQPQDFLPDPASDGFEDQVRE 120

Query: 121 LRERAKEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAE 180
           LRERA+E+PDDYFVVLVGDMITE+ALPTYQTMLNT+DGVRDETGAS  SWAIWTRAWTAE
Sbjct: 121 LRERARELPDDYFVVLVGDMITEEALPTYQTMLNTLDGVRDETGASPTSWAIWTRAWTAE 180

Query: 181 ENRHGDLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFIS 240
           ENRHGDLLNKYLYLSGRVDMRQ+EKTIQYLIGSGMDP TENNPYLGFIYTSFQERATFIS
Sbjct: 181 ENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPRTENNPYLGFIYTSFQERATFIS 240

Query: 241 HGNTARLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKN 300
           HGNTAR A E+GDIKLAQ+CGTIAADEKRHE AYTKIVEKLFEIDP+GTV+ F DMMRK 
Sbjct: 241 HGNTARQAKEHGDIKLAQICGTIAADEKRHETAYTKIVEKLFEIDPDGTVMAFADMMRKK 300

Query: 301 ITMPAHMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQK 360
           I+MPAH+MYDGR++NLFD+FS+VAQRLGVYTAKDYA+I+EFLVGRWK++ LTGLSGEG K
Sbjct: 301 ISMPAHLMYDGRNDNLFDNFSSVAQRLGVYTAKDYADILEFLVGRWKIQDLTGLSGEGNK 360

Query: 361 AQDYVCGLPARIRRLEERAQARAKEGRTIPFSWIFNREVKL 397
           AQDY+CGL  RI+RL+ERAQARAK+G  IPFSWI +REV+L
Sbjct: 361 AQDYLCGLAPRIKRLDERAQARAKKGPKIPFSWIHDREVQL 401

BLAST of CmaCh12G000830 vs. TAIR 10
Match: AT3G02630.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 601.3 bits (1549), Expect = 5.7e-172
Identity = 289/385 (75.06%), Postives = 328/385 (85.19%), Query Frame = 0

Query: 12  SPKLPSFAMPQLASFRSPKFVVASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIE 71
           SP    +   Q    RS +  +AST+RS + EV   +K +IPPREVH QV HSMPPQK+E
Sbjct: 11  SPSSYVYRPCQARGSRSSRVSMASTIRSATTEVTNGRKLYIPPREVHVQVKHSMPPQKLE 70

Query: 72  MFKCLEDWAAENILVHLKPVERCWQPQDFLPDPASEGFQEQVMELRERAKEVPDDYFVVL 131
           +FK LE WA E +L +LKPVE+ WQP DFLP+P SEGF +QV ELRER KE+PDDYFVVL
Sbjct: 71  IFKSLEGWADETLLTYLKPVEKSWQPTDFLPEPESEGFYDQVKELRERCKELPDDYFVVL 130

Query: 132 VGDMITEDALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHGDLLNKYLYLSG 191
           VGDMITE+ALPTYQTMLNT+DGVRDETGAS   WAIWTRAWTAEENRHGDLLNKYLYLSG
Sbjct: 131 VGDMITEEALPTYQTMLNTLDGVRDETGASPTPWAIWTRAWTAEENRHGDLLNKYLYLSG 190

Query: 192 RVDMRQVEKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAMENGDIKL 251
           RVDMRQ+EKTIQYLIGSGMDP TENNPYLGFIYTSFQERATFISHGNTARLA + GD+KL
Sbjct: 191 RVDMRQIEKTIQYLIGSGMDPKTENNPYLGFIYTSFQERATFISHGNTARLAKDRGDLKL 250

Query: 252 AQLCGTIAADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPAHMMYDGRDNNL 311
           AQ+CGTIAADE+RHE AYTKIVEKLFEIDP+GT+L   DMM+K I+MPAH+MYDG+D+NL
Sbjct: 251 AQICGTIAADERRHETAYTKIVEKLFEIDPDGTILGLADMMKKKISMPAHLMYDGQDDNL 310

Query: 312 FDHFSAVAQRLGVYTAKDYAEIVEFLVGRWKVERLTGLSGEGQKAQDYVCGLPARIRRLE 371
           F+HFS VAQRLGVYTAKDYA+I+EFLV RW VE LT LS EG +AQD+VCGLPARIR++E
Sbjct: 311 FEHFSTVAQRLGVYTAKDYADILEFLVERWNVETLTDLSSEGHRAQDFVCGLPARIRKIE 370

Query: 372 ERAQARAKE-GRTIPFSWIFNREVK 396
           ERAQ RAKE  + IPFSWIF R ++
Sbjct: 371 ERAQGRAKEAAKNIPFSWIFGRNIR 395

BLAST of CmaCh12G000830 vs. TAIR 10
Match: AT5G16240.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 575.9 bits (1483), Expect = 2.6e-164
Identity = 275/361 (76.18%), Postives = 315/361 (87.26%), Query Frame = 0

Query: 36  TLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDWAAENILVHLKPVERCW 95
           T+RS S  V   +K +IPPREVH QV +SMPPQK+E+FK LE WA +N+L +LKPVE+ W
Sbjct: 35  TIRSDSTAVG--RKLYIPPREVHLQVKYSMPPQKLEIFKSLEGWANDNLLAYLKPVEKSW 94

Query: 96  QPQDFLPDPASEGFQEQVMELRERAKEVPDDYFVVLVGDMITEDALPTYQTMLNTMDGVR 155
           QP DFLP+P SEGF +QV ELRER KE+ DDY +VLVGDMITE+ALPTYQTM+NT+DGVR
Sbjct: 95  QPTDFLPEPESEGFYDQVKELRERCKELSDDYLIVLVGDMITEEALPTYQTMINTLDGVR 154

Query: 156 DETGASLNSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQVEKTIQYLIGSGMDPGTE 215
           DETGAS   WA+WTRAWTAEENRHGDLLNKYLYLSGRVDMRQ+EKTIQYLIGSGMDP TE
Sbjct: 155 DETGASPTPWAVWTRAWTAEENRHGDLLNKYLYLSGRVDMRQIEKTIQYLIGSGMDPKTE 214

Query: 216 NNPYLGFIYTSFQERATFISHGNTARLAMENGDIKLAQLCGTIAADEKRHEVAYTKIVEK 275
           NNPYLGFIYTSFQERATFISHGNTARLA + GD+ L ++CGTIAADE+RHE AYTKIVEK
Sbjct: 215 NNPYLGFIYTSFQERATFISHGNTARLAKDLGDLTLGKICGTIAADERRHEHAYTKIVEK 274

Query: 276 LFEIDPEGTVLCFEDMMRKNITMPAHMMYDGRDNNLFDHFSAVAQRLGVYTAKDYAEIVE 335
           LFEIDP+ TV+ F DMMRK I+MPAH+MYDGRD+NLFDHFS+VAQRLGVYTAKDYA+I++
Sbjct: 275 LFEIDPDTTVVGFADMMRKKISMPAHLMYDGRDDNLFDHFSSVAQRLGVYTAKDYADILQ 334

Query: 336 FLVGRWKVERLTGLSGEGQKAQDYVCGLPARIRRLEERAQARAKE-GRTIPFSWIFNREV 395
            LV RW VE+L+ LS EG +AQDY+CGLPARIR+LEERAQ R KE  + IPFSWIF REV
Sbjct: 335 HLVERWNVEKLSDLSSEGNRAQDYLCGLPARIRKLEERAQGRTKEAAKNIPFSWIFGREV 393

BLAST of CmaCh12G000830 vs. TAIR 10
Match: AT3G02610.1 (Plant stearoyl-acyl-carrier-protein desaturase family protein )

HSP 1 Score: 525.0 bits (1351), Expect = 5.2e-149
Identity = 262/383 (68.41%), Postives = 308/383 (80.42%), Query Frame = 0

Query: 24  ASFRSPKFV----VASTLRSTSREVEALKKPFIPPREVHPQVTHSMPPQKIEMFKCLEDW 83
           +SF  P+ +    VA+    TS E +  KK F P +EV  QVTH++  +K+E+FK +E+W
Sbjct: 33  SSFSPPRLLRVSCVATNPSKTSEETD--KKKFRPIKEVPNQVTHTITQEKLEIFKSMENW 92

Query: 84  AAENILVHLKPVERCWQPQDFLPDPASEG-FQEQVMELRERAKEVPDDYFVVLVGDMITE 143
           A EN+L +LKPVE  WQPQDFLP+   E  F EQV ELR+R KE+PDDYFVVLVGDMITE
Sbjct: 93  AQENLLSYLKPVEASWQPQDFLPETNDEDRFYEQVKELRDRTKEIPDDYFVVLVGDMITE 152

Query: 144 DALPTYQTMLNTMDGVRDETGASLNSWAIWTRAWTAEENRHGDLLNKYLYLSGRVDMRQV 203
           +ALPTYQT LNT+DGV+DETG SL  WA+W RAWTAEENRHGDLLNKYLYLSGRVDMR V
Sbjct: 153 EALPTYQTTLNTLDGVKDETGGSLTPWAVWVRAWTAEENRHGDLLNKYLYLSGRVDMRHV 212

Query: 204 EKTIQYLIGSGMDPGTENNPYLGFIYTSFQERATFISHGNTARLAMENGDIKLAQLCGTI 263
           EKTIQYLIGSGMD   ENNPY GFIYTSFQERATFISHGNTA+LA   GD  LA++CGTI
Sbjct: 213 EKTIQYLIGSGMDSKFENNPYNGFIYTSFQERATFISHGNTAKLATTYGDTTLAKICGTI 272

Query: 264 AADEKRHEVAYTKIVEKLFEIDPEGTVLCFEDMMRKNITMPAHMMYDGRDNNLFDHFSAV 323
           AADEKRHE AYT+IVEKLFEIDP+GTV     MMRK ITMPAH+M+DGRD++LFDH++AV
Sbjct: 273 AADEKRHETAYTRIVEKLFEIDPDGTVQALASMMRKRITMPAHLMHDGRDDDLFDHYAAV 332

Query: 324 AQRLGVYTAKDYAEIVEFLVGRWKVERL-TGLSGEGQKAQDYVCGLPARIRRLEERAQAR 383
           AQR+GVYTA DYA I+EFL+ RW+VE+L  GLSGEG++AQDY+C LP RIRRLEERA  R
Sbjct: 333 AQRIGVYTATDYAGILEFLLRRWEVEKLGMGLSGEGRRAQDYLCTLPQRIRRLEERANDR 392

Query: 384 ----AKEGRTIPFSWIFNREVKL 397
               +K   ++ FSWI+ REV+L
Sbjct: 393 VKLASKSKPSVSFSWIYGREVEL 413

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P320611.2e-20687.88Acyl-[acyl-carrier-protein] desaturase, chloroplastic OS=Cucumis sativus OX=3659... [more]
P223376.9e-19984.34Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Ricinus communis ... [more]
Q428077.7e-19080.41Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Glycine max OX=38... [more]
P222432.2e-18979.80Stearoyl-[acyl-carrier-protein] 9-desaturase, chloroplastic OS=Carthamus tinctor... [more]
O228322.0e-18578.55Stearoyl-[acyl-carrier-protein] 9-desaturase 7, chloroplastic OS=Arabidopsis tha... [more]
Match NameE-valueIdentityDescription
A0A6J1KQ224.2e-231100.00Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita maxima OX=3661 GN=LOC1114965... [more]
A0A6J1GHE13.7e-21993.94Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC11145... [more]
A0A6J1BQY36.9e-21089.65Acyl-[acyl-carrier-protein] desaturase OS=Momordica charantia OX=3673 GN=LOC1110... [more]
A0A1S3BAE56.9e-21089.65Acyl-[acyl-carrier-protein] desaturase OS=Cucumis melo OX=3656 GN=LOC103487766 P... [more]
A0A6J1HDY55.0e-20889.39Acyl-[acyl-carrier-protein] desaturase OS=Cucurbita moschata OX=3662 GN=LOC11146... [more]
Match NameE-valueIdentityDescription
XP_023002825.18.6e-231100.00acyl-[acyl-carrier-protein] desaturase, chloroplastic-like [Cucurbita maxima][more]
KAG6585228.14.8e-22195.20hypothetical protein SDJN03_17961, partial [Cucurbita argyrosperma subsp. sorori... [more]
XP_023536832.16.9e-22095.20acyl-[acyl-carrier-protein] desaturase, chloroplastic-like [Cucurbita pepo subsp... [more]
XP_022951010.17.6e-21993.94acyl-[acyl-carrier-protein] desaturase, chloroplastic-like [Cucurbita moschata][more]
XP_038885157.18.4e-21090.15acyl-[acyl-carrier-protein] desaturase, chloroplastic [Benincasa hispida] >XP_03... [more]
Match NameE-valueIdentityDescription
AT2G43710.11.4e-18678.55Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT2G43710.25.3e-18678.30Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT3G02630.15.7e-17275.06Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT5G16240.12.6e-16476.18Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
AT3G02610.15.2e-14968.41Plant stearoyl-acyl-carrier-protein desaturase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR005067Fatty acid desaturase, type 2PIRSFPIRSF000346Dlt9_acylACP_descoord: 22..396
e-value: 1.7E-182
score: 604.2
IPR005067Fatty acid desaturase, type 2PFAMPF03405FA_desaturase_2coord: 69..390
e-value: 3.2E-156
score: 519.2
IPR005067Fatty acid desaturase, type 2PANTHERPTHR31155ACYL- ACYL-CARRIER-PROTEIN DESATURASE-RELATEDcoord: 19..396
IPR005067Fatty acid desaturase, type 2CDDcd01050Acyl_ACP_Desatcoord: 68..374
e-value: 0.0
score: 511.427
IPR012348Ribonucleotide reductase-likeGENE3D1.10.620.20Ribonucleotide Reductase, subunit Acoord: 51..396
e-value: 1.6E-176
score: 588.3
NoneNo IPR availablePIRSRPIRSR000346-1PIRSR000346-1coord: 2..396
e-value: 1.0E-214
score: 710.6
NoneNo IPR availablePANTHERPTHR31155:SF33ACYL-[ACYL-CARRIER-PROTEIN] DESATURASEcoord: 19..396
IPR005803Fatty acid desaturase type 2, conserved sitePROSITEPS00574FATTY_ACID_DESATUR_2coord: 316..335
IPR009078Ferritin-like superfamilySUPERFAMILY47240Ferritin-likecoord: 54..395

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G000830.1CmaCh12G000830.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006952 defense response
biological_process GO:0006633 fatty acid biosynthetic process
biological_process GO:0006631 fatty acid metabolic process
cellular_component GO:0009507 chloroplast
molecular_function GO:0045300 acyl-[acyl-carrier-protein] desaturase activity
molecular_function GO:0046872 metal ion binding
molecular_function GO:0102786 stearoyl-[acp] desaturase activity
molecular_function GO:0004768 stearoyl-CoA 9-desaturase activity
molecular_function GO:0016491 oxidoreductase activity