CmaCh12G000420 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh12G000420
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionvignain-like
LocationCma_Chr12: 161778 .. 163170 (+)
RNA-Seq ExpressionCmaCh12G000420
SyntenyCmaCh12G000420
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGCCCATTAGCAAATGCCTTTTGGTTCCATTTCTGATTGTACTAGTTTCAGGATTGGCTAAGAGCTTTGAATTCGATGAGGAGGAGTTAGCAACAGATGGAAGTCTATGGAAGCTCTATGAAAGGTGGAGTCACCACCATGCAATCTCCAGGGAACTAAAGGAGAAGCATAAACGTTACAACGTGTTTAAAGAGAATGCGAACCATGTGCTCACAGTGAATCAAATGAACAAACCATACAAGTTGAAGCTGAACAAATTCGCAGATATGTCCAATTATGAGTTTGTGAACCTGTATGCTCGCTCTAATATTACCCACTACAGGAGGTTACATGGCAGGAGAAGAGAAGGTGCCAGTGGATTCATGTACGAAAAGGCTACAGATCTTCCATCGTTCATTGATTGGAGGGAAAGAGGAGCTGTCAATGACATCAAGTATCAAGGCAGATGTGGTAACTTAGAGCTTACGATGAGGGAAAAAGAAGTTGATTACTAAATCAACATTTAATAGTTTCGAGCGTTGTCTCTATAAGAATATGATAAATTAGAGCTTACGATGTCCATGAAAAGCATTCTTCGACTTTTGAAACCACAAATAACCAATGATTTTGAAATGTATCGCAGGTAGCTGTTGGGCGTTTTCGGCTGTGGCTGCAGTTGAAGGGATCAACCAAATCAAAACCAACCAACTATTGTCTCTATCAGAGCAGGAGCTACTCGACTGCAACACAAGAAACAGAGGCTGCTATGGAGGATTCATGGAAACCGCTTATAATTTCATAAGGCGAAATGGAGGAATCGCCAGCGAGAACAACTATCCCTACCGTGGCGCAAGAGGATCCTGCCGCTCATCTAGAGTAAGTTCTGTAATTGATCTAACATCGGGAAGAAATTCAAACCTAAGATACGGTTCATCCTTCCAGAAATGATCAAACTAAGCATACATTTGTATGGTTTAGCAGATGCCTTCACCAATAGTGACAATAGATGGATTCGAAAGCGTACCTGAAAACGAGAATGCTCTGATGCAAGCCGTCGCAAACCAACCAGTGTCAGTCTCCATCGAGGCCCTAGGGAGAGATTTCCAATTCTACTGGCAGGCAAGTTGTAATAGCTTGTATCACATAGAACCTTCCATCTCTAGGCATTGTTAAATTGGAAGTGTGGGTAATTTGCAGGGAGTGTTCGATGGATATTGTGGAACAGAGCTTAATCACGGAGTGGTGGTGATCGGCTATGGAACAACCGACGGCGGAACAGACTACTGGACTGTGAGGAACTCATGGGGAGTTGGATGGGGAGAGGATGGTTACATAAGGATGAAACGTGGGGTGGAAGATCCAGAAGGTCTGTGTGGAATTGCGATGGAAGCCTCTTACCCCCTCAAGTTCTAA

mRNA sequence

ATGCCCATTAGCAAATGCCTTTTGGTTCCATTTCTGATTGTACTAGTTTCAGGATTGGCTAAGAGCTTTGAATTCGATGAGGAGGAGTTAGCAACAGATGGAAGTCTATGGAAGCTCTATGAAAGGTGGAGTCACCACCATGCAATCTCCAGGGAACTAAAGGAGAAGCATAAACGTTACAACGTGTTTAAAGAGAATGCGAACCATGTGCTCACAGTGAATCAAATGAACAAACCATACAAGTTGAAGCTGAACAAATTCGCAGATATGTCCAATTATGAGTTTGTGAACCTGTATGCTCGCTCTAATATTACCCACTACAGGAGGTTACATGGCAGGAGAAGAGAAGGTGCCAGTGGATTCATGTACGAAAAGGCTACAGATCTTCCATCGTTCATTGATTGGAGGGAAAGAGGAGCTGTCAATGACATCAAGTATCAAGGCAGATGTGGTAGCTGTTGGGCGTTTTCGGCTGTGGCTGCAGTTGAAGGGATCAACCAAATCAAAACCAACCAACTATTGTCTCTATCAGAGCAGGAGCTACTCGACTGCAACACAAGAAACAGAGGCTGCTATGGAGGATTCATGGAAACCGCTTATAATTTCATAAGGCGAAATGGAGGAATCGCCAGCGAGAACAACTATCCCTACCGTGGCGCAAGAGGATCCTGCCGCTCATCTAGAATGCCTTCACCAATAGTGACAATAGATGGATTCGAAAGCGTACCTGAAAACGAGAATGCTCTGATGCAAGCCGTCGCAAACCAACCAGTGTCAGTCTCCATCGAGGCCCTAGGGAGAGATTTCCAATTCTACTGGCAGGGAGTGTTCGATGGATATTGTGGAACAGAGCTTAATCACGGAGTGGTGGTGATCGGCTATGGAACAACCGACGGCGGAACAGACTACTGGACTGTGAGGAACTCATGGGGAGTTGGATGGGGAGAGGATGGTTACATAAGGATGAAACGTGGGGTGGAAGATCCAGAAGGTCTGTGTGGAATTGCGATGGAAGCCTCTTACCCCCTCAAGTTCTAA

Coding sequence (CDS)

ATGCCCATTAGCAAATGCCTTTTGGTTCCATTTCTGATTGTACTAGTTTCAGGATTGGCTAAGAGCTTTGAATTCGATGAGGAGGAGTTAGCAACAGATGGAAGTCTATGGAAGCTCTATGAAAGGTGGAGTCACCACCATGCAATCTCCAGGGAACTAAAGGAGAAGCATAAACGTTACAACGTGTTTAAAGAGAATGCGAACCATGTGCTCACAGTGAATCAAATGAACAAACCATACAAGTTGAAGCTGAACAAATTCGCAGATATGTCCAATTATGAGTTTGTGAACCTGTATGCTCGCTCTAATATTACCCACTACAGGAGGTTACATGGCAGGAGAAGAGAAGGTGCCAGTGGATTCATGTACGAAAAGGCTACAGATCTTCCATCGTTCATTGATTGGAGGGAAAGAGGAGCTGTCAATGACATCAAGTATCAAGGCAGATGTGGTAGCTGTTGGGCGTTTTCGGCTGTGGCTGCAGTTGAAGGGATCAACCAAATCAAAACCAACCAACTATTGTCTCTATCAGAGCAGGAGCTACTCGACTGCAACACAAGAAACAGAGGCTGCTATGGAGGATTCATGGAAACCGCTTATAATTTCATAAGGCGAAATGGAGGAATCGCCAGCGAGAACAACTATCCCTACCGTGGCGCAAGAGGATCCTGCCGCTCATCTAGAATGCCTTCACCAATAGTGACAATAGATGGATTCGAAAGCGTACCTGAAAACGAGAATGCTCTGATGCAAGCCGTCGCAAACCAACCAGTGTCAGTCTCCATCGAGGCCCTAGGGAGAGATTTCCAATTCTACTGGCAGGGAGTGTTCGATGGATATTGTGGAACAGAGCTTAATCACGGAGTGGTGGTGATCGGCTATGGAACAACCGACGGCGGAACAGACTACTGGACTGTGAGGAACTCATGGGGAGTTGGATGGGGAGAGGATGGTTACATAAGGATGAAACGTGGGGTGGAAGATCCAGAAGGTCTGTGTGGAATTGCGATGGAAGCCTCTTACCCCCTCAAGTTCTAA

Protein sequence

MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF
Homology
BLAST of CmaCh12G000420 vs. ExPASy Swiss-Prot
Match: O65039 (Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1)

HSP 1 Score: 437.6 bits (1124), Expect = 1.3e-121
Identity = 209/344 (60.76%), Postives = 264/344 (76.74%), Query Frame = 0

Query: 3   ISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNV 62
           + K +L+   + LV  + +SF+F E+EL ++ SLW LYERW  HH +SR L EK KR+NV
Sbjct: 1   MQKFILLALSLALVLAITESFDFHEKELESEESLWGLYERWRSHHTVSRSLHEKQKRFNV 60

Query: 63  FKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFM 122
           FK NA HV   N+M+KPYKLKLNKFADM+N+EF N Y+ S + H+R   G  R G   FM
Sbjct: 61  FKHNAMHVHNANKMDKPYKLKLNKFADMTNHEFRNTYSGSKVKHHRMFRGGPR-GNGTFM 120

Query: 123 YEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELL 182
           YEK   +P+ +DWR++GAV  +K QG+CGSCWAFS + AVEGINQIKTN+L+SLSEQEL+
Sbjct: 121 YEKVDTVPASVDWRKKGAVTSVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQELV 180

Query: 183 DCNT-RNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFES 242
           DC+T +N+GC GG M+ A+ FI++ GGI +E NYPY    G+C  S+  +P V+IDG E+
Sbjct: 181 DCDTDQNQGCNGGLMDYAFEFIKQRGGITTEANYPYEAYDGTCDVSKENAPAVSIDGHEN 240

Query: 243 VPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 302
           VPEN ENAL++AVANQPVSV+I+A G DFQFY +GVF G CGTEL+HGV ++GYGTT  G
Sbjct: 241 VPENDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGSCGTELDHGVAIVGYGTTIDG 300

Query: 303 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           T YWTV+NSWG  WGE GYIRM+RG+ D EGLCGIAMEASYP+K
Sbjct: 301 TKYWTVKNSWGPEWGEKGYIRMERGISDKEGLCGIAMEASYPIK 343

BLAST of CmaCh12G000420 vs. ExPASy Swiss-Prot
Match: P12412 (Vignain OS=Vigna mungo OX=3915 PE=1 SV=1)

HSP 1 Score: 429.5 bits (1103), Expect = 3.6e-119
Identity = 205/346 (59.25%), Postives = 261/346 (75.43%), Query Frame = 0

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M + K L V   + LV G+A SF+F E++L ++ SLW LYERW  HH +SR L EKHKR+
Sbjct: 1   MAMKKLLWVVLSLSLVLGVANSFDFHEKDLESEESLWDLYERWRSHHTVSRSLGEKHKRF 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFK N  HV   N+M+KPYKLKLNKFADM+N+EF + YA S + H++   G  + G+  
Sbjct: 61  NVFKANVMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHHKMFRG-SQHGSGT 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEK   +P+ +DWR++GAV D+K QG+CGSCWAFS + AVEGINQIKTN+L+SLSEQE
Sbjct: 121 FMYEKVGSVPASVDWRKKGAVTDVKDQGQCGSCWAFSTIVAVEGINQIKTNKLVSLSEQE 180

Query: 181 LLDCN-TRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           L+DC+   N+GC GG ME+A+ FI++ GGI +E+NYPY    G+C  S++    V+IDG 
Sbjct: 181 LVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYTAQEGTCDESKVNDLAVSIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 300
           E+VP N ENAL++AVANQPVSV+I+A G DFQFY +GVF G C T+LNHGV ++GYGTT 
Sbjct: 241 ENVPVNDENALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCNTDLNHGVAIVGYGTTV 300

Query: 301 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            GT+YW VRNSWG  WGE GYIRM+R +   EGLCGIAM ASYP+K
Sbjct: 301 DGTNYWIVRNSWGPEWGEQGYIRMQRNISKKEGLCGIAMMASYPIK 345

BLAST of CmaCh12G000420 vs. ExPASy Swiss-Prot
Match: P25803 (Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2)

HSP 1 Score: 423.3 bits (1087), Expect = 2.6e-117
Identity = 204/346 (58.96%), Postives = 258/346 (74.57%), Query Frame = 0

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M   K L V     LV G+A SF+F +++LA++ SLW LYERW  HH +SR L EKHKR+
Sbjct: 1   MATKKLLWVVLSFSLVLGVANSFDFHDKDLASEESLWDLYERWRSHHTVSRSLGEKHKRF 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFK N  HV   N+M+KPYKLKLNKFADM+N+EF + YA S + H R   G   E  + 
Sbjct: 61  NVFKANLMHVHNTNKMDKPYKLKLNKFADMTNHEFRSTYAGSKVNHPRMFRGTPHENGA- 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEK   +P  +DWR++GAV D+K QG+CGSCWAFS V AVEGINQIKTN+L++LSEQE
Sbjct: 121 FMYEKVVSVPPSVDWRKKGAVTDVKDQGQCGSCWAFSTVVAVEGINQIKTNKLVALSEQE 180

Query: 181 LLDCN-TRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           L+DC+   N+GC GG ME+A+ FI++ GGI +E+NYPY+   G+C +S++    V+IDG 
Sbjct: 181 LVDCDKEENQGCNGGLMESAFEFIKQKGGITTESNYPYKAQEGTCDASKVNDLAVSIDGH 240

Query: 241 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 300
           E+VP N E+AL++AVANQPVSV+I+A G DFQFY +GVF G C T+LNHGV ++GYGTT 
Sbjct: 241 ENVPANDEDALLKAVANQPVSVAIDAGGSDFQFYSEGVFTGDCSTDLNHGVAIVGYGTTV 300

Query: 301 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            GT+YW VRNSWG  WGE GYIRM+R +   EGLCGIAM  SYP+K
Sbjct: 301 DGTNYWIVRNSWGPEWGEHGYIRMQRNISKKEGLCGIAMLPSYPIK 345

BLAST of CmaCh12G000420 vs. ExPASy Swiss-Prot
Match: Q9STL5 (KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 PE=2 SV=1)

HSP 1 Score: 406.0 bits (1042), Expect = 4.3e-112
Identity = 199/342 (58.19%), Postives = 255/342 (74.56%), Query Frame = 0

Query: 11  FLIVLVSGL-----AKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNVFKE 70
           F IVL+S L     +K F+FDE+EL T+ ++WKLYERW  HH++SR   E  KR+NVF+ 
Sbjct: 4   FFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRH 63

Query: 71  NANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYEK 130
           N  HV   N+ NKPYKLK+N+FAD++++EF + YA SN+ H+R L G +R G+ GFMYE 
Sbjct: 64  NVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKR-GSGGFMYEN 123

Query: 131 ATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDCN 190
            T +PS +DWRE+GAV ++K Q  CGSCWAFS VAAVEGIN+I+TN+L+SLSEQEL+DC+
Sbjct: 124 VTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCD 183

Query: 191 T-RNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGS-CRSSRMPSPIVTIDGFESVP 250
           T  N+GC GG ME A+ FI+ NGGI +E  YPY  +    CR++ +    VTIDG E VP
Sbjct: 184 TEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVP 243

Query: 251 EN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGTD 310
           EN E  L++AVA+QPVSV+I+A   DFQ Y +GVF G CGT+LNHGVV++GYG T  GT 
Sbjct: 244 ENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGETKNGTK 303

Query: 311 YWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           YW VRNSWG  WGE GY+R++RG+ + EG CGIAMEASYP K
Sbjct: 304 YWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CmaCh12G000420 vs. ExPASy Swiss-Prot
Match: Q9STL4 (KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 PE=1 SV=1)

HSP 1 Score: 402.5 bits (1033), Expect = 4.8e-111
Identity = 196/346 (56.65%), Postives = 257/346 (74.28%), Query Frame = 0

Query: 3   ISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNV 62
           + K LL+    +++   A  F++D++E+ ++  L  LY+RW  HH++ R L E+ KR+NV
Sbjct: 1   MKKLLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNV 60

Query: 63  FKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFM 122
           F+ N  HV   N+ N+ YKLKLNKFAD++  EF N Y  SNI H+R L G +R G+  FM
Sbjct: 61  FRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKR-GSKQFM 120

Query: 123 Y--EKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 182
           Y  E  + LPS +DWR++GAV +IK QG+CGSCWAFS VAAVEGIN+IKTN+L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 LLDCNTR-NRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 242
           L+DC+T+ N GC GG ME A+ FI++NGGI +E++YPY G  G C +S+    +VTIDG 
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 302
           E VPEN ENAL++AVANQPVSV+I+A   DFQFY +GVF G CGTELNHGV  +GYG ++
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYG-SE 300

Query: 303 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            G  YW VRNSWG  WGE GYI+++R +++PEG CGIAMEASYP+K
Sbjct: 301 RGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CmaCh12G000420 vs. ExPASy TrEMBL
Match: A0A6J1KIL0 (vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111496079 PE=3 SV=1)

HSP 1 Score: 718.8 bits (1854), Expect = 1.1e-203
Identity = 345/345 (100.00%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY
Sbjct: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG
Sbjct: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE
Sbjct: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240
           LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE
Sbjct: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240

Query: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 300
           SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG
Sbjct: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 300

Query: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF
Sbjct: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 345

BLAST of CmaCh12G000420 vs. ExPASy TrEMBL
Match: A0A6J1GHN5 (vignain-like OS=Cucurbita moschata OX=3662 GN=LOC111454276 PE=3 SV=1)

HSP 1 Score: 701.8 bits (1810), Expect = 1.4e-198
Identity = 337/345 (97.68%), Postives = 341/345 (98.84%), Query Frame = 0

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M ISKCLLVPFLIVL+SGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY
Sbjct: 1   MAISKCLLVPFLIVLISGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVN+YARSNITHYRRLHG+RREGASG
Sbjct: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNVYARSNITHYRRLHGKRREGASG 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEKATDLPSFIDWRERGAV+DIK QGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE
Sbjct: 121 FMYEKATDLPSFIDWRERGAVSDIKDQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240
           LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE
Sbjct: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240

Query: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 300
           SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDG CGTELNHGVVVIGYGTTDGG
Sbjct: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGDCGTELNHGVVVIGYGTTDGG 300

Query: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGI MEASYPLKF
Sbjct: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIVMEASYPLKF 345

BLAST of CmaCh12G000420 vs. ExPASy TrEMBL
Match: A0A1S3BA70 (vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1)

HSP 1 Score: 595.9 bits (1535), Expect = 1.1e-166
Identity = 285/346 (82.37%), Postives = 314/346 (90.75%), Query Frame = 0

Query: 1   MPISKCLLVP-FLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60
           M I K LLVP  LIVLVSGLA+SFEFDE+ELAT+ SLW+LYERW +HH ISR LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGNHHTISRNLKEKHKR 60

Query: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGAS 120
           ++VFKEN NHV TVNQMNKPYKLKLNKFADMSNYEFVN YARSNI+H+R+LHGRRR GA 
Sbjct: 61  FSVFKENVNHVFTVNQMNKPYKLKLNKFADMSNYEFVNFYARSNISHFRKLHGRRR-GAG 120

Query: 121 GFMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180
           GFMYE+ TDLPS +DWRERGAVN IK QG CGSCWAFS+VAAVE IN+IKTNQLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAIKEQGTCGSCWAFSSVAAVEAINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           ELLDCN RN+GC GGFME A++FI+RNGGIA+EN+YPY G+RG CRSSR+ SPIV IDG+
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDG 300
           ESVPENE+ALMQAVANQPVSV+I+A GRDFQFYWQGVFDGYCGTELNHGVV IGYGTT+ 
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYWQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           GTDYW VRNSWGVGWGEDGY+RMKRGVE PEGLCGIAMEASYP+KF
Sbjct: 301 GTDYWIVRNSWGVGWGEDGYVRMKRGVEQPEGLCGIAMEASYPIKF 345

BLAST of CmaCh12G000420 vs. ExPASy TrEMBL
Match: A0A0A0LMU4 (Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1)

HSP 1 Score: 586.3 bits (1510), Expect = 8.6e-164
Identity = 282/346 (81.50%), Postives = 312/346 (90.17%), Query Frame = 0

Query: 1   MPISKCLLVP-FLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60
           M I K LLVP  LIVLVSGLA+SFEFDE+ELAT+ SLW+LYERW  HH ISR LKEKHKR
Sbjct: 1   MAIGKFLLVPLLLIVLVSGLAESFEFDEKELATEESLWQLYERWGKHHTISRNLKEKHKR 60

Query: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGAS 120
           ++VFKEN NHV TVNQM+KPYKLKLNKFADMSNYEFVN YARSNI+HYR+LH RRR GA 
Sbjct: 61  FSVFKENVNHVFTVNQMDKPYKLKLNKFADMSNYEFVNFYARSNISHYRKLHERRR-GAG 120

Query: 121 GFMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180
           GFMYE+ TDLPS +DWRERGAVN +K QGRCGSCWAFS+VAAVEGIN+IKTNQLLSLSEQ
Sbjct: 121 GFMYEQDTDLPSSVDWRERGAVNAVKEQGRCGSCWAFSSVAAVEGINKIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           ELLDCN RN+GC GGFME A++FI+RNGGIA+EN+YPY G+RG CRSSR+ SPIV IDG+
Sbjct: 181 ELLDCNYRNKGCNGGFMEIAFDFIKRNGGIATENSYPYHGSRGLCRSSRISSPIVKIDGY 240

Query: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDG 300
           ESVPENE+ALMQAVANQPVSV+I+A GRDFQFY QGVFDGYCGTELNHGVV IGYGTT+ 
Sbjct: 241 ESVPENEDALMQAVANQPVSVAIDAAGRDFQFYSQGVFDGYCGTELNHGVVAIGYGTTED 300

Query: 301 GTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           GTDYW VRNSWGVGWGEDGY+RMKRGVE  EGLCGIAMEASYP+K+
Sbjct: 301 GTDYWLVRNSWGVGWGEDGYVRMKRGVEQAEGLCGIAMEASYPIKY 345

BLAST of CmaCh12G000420 vs. ExPASy TrEMBL
Match: A0A6J1K7P4 (vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111491900 PE=3 SV=1)

HSP 1 Score: 578.2 bits (1489), Expect = 2.3e-161
Identity = 285/376 (75.80%), Postives = 315/376 (83.78%), Query Frame = 0

Query: 1   MPISKCLLVPF-LIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKR 60
           M ISK +LVPF LIVLVSGL +SFEFDE+ELAT+ SLW+LYERW +HH ISR LKEKHKR
Sbjct: 1   MAISKFILVPFLLIVLVSGLGESFEFDEKELATEESLWRLYERWGNHHTISRGLKEKHKR 60

Query: 61  YNVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGAS 120
           +NVFKEN NHV TVNQMNKPYKLKLNKFADMSN EFV+ YARSNI+HYR+LHG+R   + 
Sbjct: 61  FNVFKENVNHVFTVNQMNKPYKLKLNKFADMSNSEFVSFYARSNISHYRKLHGKR---SG 120

Query: 121 GFMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQ 180
           GFMYE+ATDLPS+IDWRERGAVNDIK QG+CGSCWAFS VAAVEGINQIKTNQLLSLSEQ
Sbjct: 121 GFMYEQATDLPSYIDWRERGAVNDIKEQGKCGSCWAFSTVAAVEGINQIKTNQLLSLSEQ 180

Query: 181 ELLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 240
           ELLDCN RN+GC GGFME A++FI+RNGGIA+ENNYPY GARG CRSSR+ SP VTIDG+
Sbjct: 181 ELLDCNFRNKGCNGGFMEIAFDFIKRNGGIATENNYPYHGARGLCRSSRISSPTVTIDGY 240

Query: 241 ESVPENENALMQAVANQPVSVSIEALGRDFQFY------------W-------------- 300
           ESVPENENALMQAVANQPVSV+I+A+GRDFQFY            W              
Sbjct: 241 ESVPENENALMQAVANQPVSVAIDAVGRDFQFYSQASLGFIREIDWLTIIISSGNVNLKS 300

Query: 301 ----QGVFDGYCGTELNHGVVVIGYGTTDGGTDYWTVRNSWGVGWGEDGYIRMKRGVEDP 346
               QGVFDGYCGTELNHGVV IGYGTT+ GTDYW VRNSWGVGWGE+GY+RMKRGVE  
Sbjct: 301 VGKVQGVFDGYCGTELNHGVVAIGYGTTEEGTDYWMVRNSWGVGWGEEGYVRMKRGVEKA 360

BLAST of CmaCh12G000420 vs. NCBI nr
Match: XP_023002122.1 (vignain-like [Cucurbita maxima])

HSP 1 Score: 718.8 bits (1854), Expect = 2.3e-203
Identity = 345/345 (100.00%), Postives = 345/345 (100.00%), Query Frame = 0

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY
Sbjct: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG
Sbjct: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE
Sbjct: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240
           LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE
Sbjct: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240

Query: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 300
           SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG
Sbjct: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 300

Query: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF
Sbjct: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 345

BLAST of CmaCh12G000420 vs. NCBI nr
Match: XP_022951466.1 (vignain-like [Cucurbita moschata])

HSP 1 Score: 701.8 bits (1810), Expect = 2.9e-198
Identity = 337/345 (97.68%), Postives = 341/345 (98.84%), Query Frame = 0

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M ISKCLLVPFLIVL+SGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY
Sbjct: 1   MAISKCLLVPFLIVLISGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVN+YARSNITHYRRLHG+RREGASG
Sbjct: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNVYARSNITHYRRLHGKRREGASG 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEKATDLPSFIDWRERGAV+DIK QGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE
Sbjct: 121 FMYEKATDLPSFIDWRERGAVSDIKDQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240
           LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE
Sbjct: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240

Query: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 300
           SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDG CGTELNHGVVVIGYGTTDGG
Sbjct: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGDCGTELNHGVVVIGYGTTDGG 300

Query: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGI MEASYPLKF
Sbjct: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIVMEASYPLKF 345

BLAST of CmaCh12G000420 vs. NCBI nr
Match: XP_023537428.1 (vignain-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 699.1 bits (1803), Expect = 1.9e-197
Identity = 337/345 (97.68%), Postives = 340/345 (98.55%), Query Frame = 0

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M ISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY
Sbjct: 1   MAISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHG+RREGASG
Sbjct: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGKRREGASG 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEKATDLPSFIDWRERGAV+DIK QGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE
Sbjct: 121 FMYEKATDLPSFIDWRERGAVSDIKDQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240
           LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE
Sbjct: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240

Query: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 300
           SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDG CGTELNHGVVVIGYGTTD G
Sbjct: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGDCGTELNHGVVVIGYGTTDDG 300

Query: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           TDYW+VRNSWGVGWGEDGYIRMKRGVEDPEGLCGI MEASYPLKF
Sbjct: 301 TDYWSVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIVMEASYPLKF 345

BLAST of CmaCh12G000420 vs. NCBI nr
Match: KAG6585186.1 (hypothetical protein SDJN03_17919, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 696.0 bits (1795), Expect = 1.6e-196
Identity = 334/345 (96.81%), Postives = 339/345 (98.26%), Query Frame = 0

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M ISKCLLVPFLIVL+SGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY
Sbjct: 1   MAISKCLLVPFLIVLISGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVN+YARSNITHYRRLHG+RRE ASG
Sbjct: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNVYARSNITHYRRLHGKRRESASG 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEKATDLPSFIDWRERGAV+DIK QGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE
Sbjct: 121 FMYEKATDLPSFIDWRERGAVSDIKDQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240
           LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE
Sbjct: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240

Query: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 300
           SVPENENALMQAVANQPVSVSIEALGRDFQFYWQ +FDG CGTELNHGVVVIGYGTTDGG
Sbjct: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQAMFDGDCGTELNHGVVVIGYGTTDGG 300

Query: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGI MEASYPLKF
Sbjct: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIVMEASYPLKF 345

BLAST of CmaCh12G000420 vs. NCBI nr
Match: KAG7020106.1 (hypothetical protein SDJN02_16788, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 657.5 bits (1695), Expect = 6.2e-185
Identity = 321/345 (93.04%), Postives = 325/345 (94.20%), Query Frame = 0

Query: 1   MPISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRY 60
           M ISKCLLVPFLIVL+SGLAKSFEFDEEEL TDGSLWKLYERWSHHHAISRELKEKHKRY
Sbjct: 1   MAISKCLLVPFLIVLISGLAKSFEFDEEELETDGSLWKLYERWSHHHAISRELKEKHKRY 60

Query: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASG 120
           NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVN+YARSNITHYRRLHG+RREGASG
Sbjct: 61  NVFKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNVYARSNITHYRRLHGKRREGASG 120

Query: 121 FMYEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180
           FMYEKATDLPSFIDWRERGAV+DIK QGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE
Sbjct: 121 FMYEKATDLPSFIDWRERGAVSDIKDQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 180

Query: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240
           LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE
Sbjct: 181 LLDCNTRNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFE 240

Query: 241 SVPENENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 300
           SVPENENALMQAVANQPVS               GVFDG CGTELNHGVVVIGYGTTDGG
Sbjct: 241 SVPENENALMQAVANQPVS---------------GVFDGDCGTELNHGVVVIGYGTTDGG 300

Query: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLKF 346
           TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGI MEASYPLKF
Sbjct: 301 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIVMEASYPLKF 330

BLAST of CmaCh12G000420 vs. TAIR 10
Match: AT3G48350.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 406.0 bits (1042), Expect = 3.1e-113
Identity = 199/342 (58.19%), Postives = 255/342 (74.56%), Query Frame = 0

Query: 11  FLIVLVSGL-----AKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNVFKE 70
           F IVL+S L     +K F+FDE+EL T+ ++WKLYERW  HH++SR   E  KR+NVF+ 
Sbjct: 4   FFIVLISFLSLLQASKGFDFDEKELETEENVWKLYERWRGHHSVSRASHEAIKRFNVFRH 63

Query: 71  NANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYEK 130
           N  HV   N+ NKPYKLK+N+FAD++++EF + YA SN+ H+R L G +R G+ GFMYE 
Sbjct: 64  NVLHVHRTNKKNKPYKLKINRFADITHHEFRSSYAGSNVKHHRMLRGPKR-GSGGFMYEN 123

Query: 131 ATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDCN 190
            T +PS +DWRE+GAV ++K Q  CGSCWAFS VAAVEGIN+I+TN+L+SLSEQEL+DC+
Sbjct: 124 VTRVPSSVDWREKGAVTEVKNQQDCGSCWAFSTVAAVEGINKIRTNKLVSLSEQELVDCD 183

Query: 191 T-RNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGS-CRSSRMPSPIVTIDGFESVP 250
           T  N+GC GG ME A+ FI+ NGGI +E  YPY  +    CR++ +    VTIDG E VP
Sbjct: 184 TEENQGCAGGLMEPAFEFIKNNGGIKTEETYPYDSSDVQFCRANSIGGETVTIDGHEHVP 243

Query: 251 EN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGTD 310
           EN E  L++AVA+QPVSV+I+A   DFQ Y +GVF G CGT+LNHGVV++GYG T  GT 
Sbjct: 244 ENDEEELLKAVAHQPVSVAIDAGSSDFQLYSEGVFIGECGTQLNHGVVIVGYGETKNGTK 303

Query: 311 YWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           YW VRNSWG  WGE GY+R++RG+ + EG CGIAMEASYP K
Sbjct: 304 YWIVRNSWGPEWGEGGYVRIERGISENEGRCGIAMEASYPTK 344

BLAST of CmaCh12G000420 vs. TAIR 10
Match: AT3G48340.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 402.5 bits (1033), Expect = 3.4e-112
Identity = 196/346 (56.65%), Postives = 257/346 (74.28%), Query Frame = 0

Query: 3   ISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNV 62
           + K LL+    +++   A  F++D++E+ ++  L  LY+RW  HH++ R L E+ KR+NV
Sbjct: 1   MKKLLLIFLFSLVILQTACGFDYDDKEIESEEGLSTLYDRWRSHHSVPRSLNEREKRFNV 60

Query: 63  FKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFM 122
           F+ N  HV   N+ N+ YKLKLNKFAD++  EF N Y  SNI H+R L G +R G+  FM
Sbjct: 61  FRHNVMHVHNTNKKNRSYKLKLNKFADLTINEFKNAYTGSNIKHHRMLQGPKR-GSKQFM 120

Query: 123 Y--EKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQE 182
           Y  E  + LPS +DWR++GAV +IK QG+CGSCWAFS VAAVEGIN+IKTN+L+SLSEQE
Sbjct: 121 YDHENLSKLPSSVDWRKKGAVTEIKNQGKCGSCWAFSTVAAVEGINKIKTNKLVSLSEQE 180

Query: 183 LLDCNTR-NRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGF 242
           L+DC+T+ N GC GG ME A+ FI++NGGI +E++YPY G  G C +S+    +VTIDG 
Sbjct: 181 LVDCDTKQNEGCNGGLMEIAFEFIKKNGGITTEDSYPYEGIDGKCDASKDNGVLVTIDGH 240

Query: 243 ESVPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTD 302
           E VPEN ENAL++AVANQPVSV+I+A   DFQFY +GVF G CGTELNHGV  +GYG ++
Sbjct: 241 EDVPENDENALLKAVANQPVSVAIDAGSSDFQFYSEGVFTGSCGTELNHGVAAVGYG-SE 300

Query: 303 GGTDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
            G  YW VRNSWG  WGE GYI+++R +++PEG CGIAMEASYP+K
Sbjct: 301 RGKKYWIVRNSWGAEWGEGGYIKIEREIDEPEGRCGIAMEASYPIK 344

BLAST of CmaCh12G000420 vs. TAIR 10
Match: AT5G50260.1 (Cysteine proteinases superfamily protein )

HSP 1 Score: 399.8 bits (1026), Expect = 2.2e-111
Identity = 189/344 (54.94%), Postives = 250/344 (72.67%), Query Frame = 0

Query: 3   ISKCLLVPFLIVLVSGLAKSFEFDEEELATDGSLWKLYERWSHHHAISRELKEKHKRYNV 62
           + + +++   +++V    K  +F  +++ ++ SLW+LYERW  HH ++R L+EK KR+NV
Sbjct: 1   MKRFIVLALCMLMVLETTKGLDFHNKDVESENSLWELYERWRSHHTVARSLEEKAKRFNV 60

Query: 63  FKENANHVLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFM 122
           FK N  H+   N+ +K YKLKLNKF DM++ EF   YA SNI H+R   G ++     FM
Sbjct: 61  FKHNVKHIHETNKKDKSYKLKLNKFGDMTSEEFRRTYAGSNIKHHRMFQGEKK-ATKSFM 120

Query: 123 YEKATDLPSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELL 182
           Y     LP+ +DWR+ GAV  +K QG+CGSCWAFS V AVEGINQI+T +L SLSEQEL+
Sbjct: 121 YANVNTLPTSVDWRKNGAVTPVKNQGQCGSCWAFSTVVAVEGINQIRTKKLTSLSEQELV 180

Query: 183 DCNT-RNRGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFES 242
           DC+T +N+GC GG M+ A+ FI+  GG+ SE  YPY+ +  +C +++  +P+V+IDG E 
Sbjct: 181 DCDTNQNQGCNGGLMDLAFEFIKEKGGLTSELVYPYKASDETCDTNKENAPVVSIDGHED 240

Query: 243 VPEN-ENALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGG 302
           VP+N E+ LM+AVANQPVSV+I+A G DFQFY +GVF G CGTELNHGV V+GYGTT  G
Sbjct: 241 VPKNSEDDLMKAVANQPVSVAIDAGGSDFQFYSEGVFTGRCGTELNHGVAVVGYGTTIDG 300

Query: 303 TDYWTVRNSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           T YW V+NSWG  WGE GYIRM+RG+   EGLCGIAMEASYPLK
Sbjct: 301 TKYWIVKNSWGEEWGEKGYIRMQRGIRHKEGLCGIAMEASYPLK 343

BLAST of CmaCh12G000420 vs. TAIR 10
Match: AT5G43060.1 (Granulin repeat cysteine protease family protein )

HSP 1 Score: 315.5 bits (807), Expect = 5.4e-86
Identity = 161/324 (49.69%), Postives = 213/324 (65.74%), Query Frame = 0

Query: 28  EELATDGSLWKLYERWSHHHAISRELK-----EKHKRYNVFKENANHVLTVNQMNKPYKL 87
           E   +D  + ++YE W   H   +  +     EK +R+ +FK+N   +   N  N  YKL
Sbjct: 38  ETSRSDSEVERIYEAWMVEHGKKKMNQNGLGAEKDQRFEIFKDNLRFIDEHNTKNLSYKL 97

Query: 88  KLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYEKATDLPSFIDWRERGAVN 147
            L +FAD++N E+ ++Y  +  T       R  + +  +       LP  +DWR+ GAV 
Sbjct: 98  GLTRFADLTNEEYRSMYLGAKPTK------RVLKTSDRYQARVGDALPDSVDWRKEGAVA 157

Query: 148 DIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDCNTR-NRGCYGGFMETAYN 207
           D+K QG CGSCWAFS + AVEGIN+I T  L+SLSEQEL+DC+T  N+GC GG M+ A+ 
Sbjct: 158 DVKDQGSCGSCWAFSTIGAVEGINKIVTGDLISLSEQELVDCDTSYNQGCNGGLMDYAFE 217

Query: 208 FIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFESVPEN-ENALMQAVANQPVSV 267
           FI +NGGI +E +YPY+ A G C  +R  + +VTID +E VPEN E +L +A+A+QP+SV
Sbjct: 218 FIIKNGGIDTEADYPYKAADGRCDQNRKNAKVVTIDSYEDVPENSEASLKKALAHQPISV 277

Query: 268 SIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGTDYWTVRNSWGVGWGEDGYI 327
           +IEA GR FQ Y  GVFDG CGTEL+HGVV +GYG T+ G DYW VRNSWG  WGE GYI
Sbjct: 278 AIEAGGRAFQLYSSGVFDGLCGTELDHGVVAVGYG-TENGKDYWIVRNSWGNRWGESGYI 337

Query: 328 RMKRGVEDPEGLCGIAMEASYPLK 345
           +M R +E P G CGIAMEASYP+K
Sbjct: 338 KMARNIEAPTGKCGIAMEASYPIK 354

BLAST of CmaCh12G000420 vs. TAIR 10
Match: AT4G35350.1 (xylem cysteine peptidase 1 )

HSP 1 Score: 307.8 bits (787), Expect = 1.1e-83
Identity = 163/337 (48.37%), Postives = 217/337 (64.39%), Query Frame = 0

Query: 14  VLVSGLAKSFE---FDEEELATDGSLWKLYERW-SHHHAISRELKEKHKRYNVFKENANH 73
           +L    A+ F    +  E L     L +L+E W S H    + ++EK  R+ VF+EN  H
Sbjct: 22  LLCCAFARDFSIVGYTPEHLTNTDKLLELFESWMSEHSKAYKSVEEKVHRFEVFRENLMH 81

Query: 74  VLTVNQMNKPYKLKLNKFADMSNYEFVNLYARSNITHYRRLHGRRREGASGFMYEKATDL 133
           +   N     Y L LN+FAD+++ EF   Y    +   +    R+R+ ++ F Y   TDL
Sbjct: 82  IDQRNNEINSYWLGLNEFADLTHEEFKGRY----LGLAKPQFSRKRQPSANFRYRDITDL 141

Query: 134 PSFIDWRERGAVNDIKYQGRCGSCWAFSAVAAVEGINQIKTNQLLSLSEQELLDCNTR-N 193
           P  +DWR++GAV  +K QG+CGSCWAFS VAAVEGINQI T  L SLSEQEL+DC+T  N
Sbjct: 142 PKSVDWRKKGAVAPVKDQGQCGSCWAFSTVAAVEGINQITTGNLSSLSEQELIDCDTTFN 201

Query: 194 RGCYGGFMETAYNFIRRNGGIASENNYPYRGARGSCRSSRMPSPIVTIDGFESVPENEN- 253
            GC GG M+ A+ +I   GG+  E++YPY    G C+  +     VTI G+E VPEN++ 
Sbjct: 202 SGCNGGLMDYAFQYIISTGGLHKEDDYPYLMEEGICQEQKEDVERVTISGYEDVPENDDE 261

Query: 254 ALMQAVANQPVSVSIEALGRDFQFYWQGVFDGYCGTELNHGVVVIGYGTTDGGTDYWTVR 313
           +L++A+A+QPVSV+IEA GRDFQFY  GVF+G CGT+L+HGV  +GYG++  G+DY  V+
Sbjct: 262 SLVKALAHQPVSVAIEASGRDFQFYKGGVFNGKCGTDLDHGVAAVGYGSSK-GSDYVIVK 321

Query: 314 NSWGVGWGEDGYIRMKRGVEDPEGLCGIAMEASYPLK 345
           NSWG  WGE G+IRMKR    PEGLCGI   ASYP K
Sbjct: 322 NSWGPRWGEKGFIRMKRNTGKPEGLCGINKMASYPTK 353

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O650391.3e-12160.76Vignain OS=Ricinus communis OX=3988 GN=CYSEP PE=1 SV=1[more]
P124123.6e-11959.25Vignain OS=Vigna mungo OX=3915 PE=1 SV=1[more]
P258032.6e-11758.96Vignain OS=Phaseolus vulgaris OX=3885 PE=2 SV=2[more]
Q9STL54.3e-11258.19KDEL-tailed cysteine endopeptidase CEP3 OS=Arabidopsis thaliana OX=3702 GN=CEP3 ... [more]
Q9STL44.8e-11156.65KDEL-tailed cysteine endopeptidase CEP2 OS=Arabidopsis thaliana OX=3702 GN=CEP2 ... [more]
Match NameE-valueIdentityDescription
A0A6J1KIL01.1e-203100.00vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111496079 PE=3 SV=1[more]
A0A6J1GHN51.4e-19897.68vignain-like OS=Cucurbita moschata OX=3662 GN=LOC111454276 PE=3 SV=1[more]
A0A1S3BA701.1e-16682.37vignain-like OS=Cucumis melo OX=3656 GN=LOC103487731 PE=3 SV=1[more]
A0A0A0LMU48.6e-16481.50Uncharacterized protein OS=Cucumis sativus OX=3659 GN=Csa_2G349680 PE=3 SV=1[more]
A0A6J1K7P42.3e-16175.80vignain-like OS=Cucurbita maxima OX=3661 GN=LOC111491900 PE=3 SV=1[more]
Match NameE-valueIdentityDescription
XP_023002122.12.3e-203100.00vignain-like [Cucurbita maxima][more]
XP_022951466.12.9e-19897.68vignain-like [Cucurbita moschata][more]
XP_023537428.11.9e-19797.68vignain-like [Cucurbita pepo subsp. pepo][more]
KAG6585186.11.6e-19696.81hypothetical protein SDJN03_17919, partial [Cucurbita argyrosperma subsp. sorori... [more]
KAG7020106.16.2e-18593.04hypothetical protein SDJN02_16788, partial [Cucurbita argyrosperma subsp. argyro... [more]
Match NameE-valueIdentityDescription
AT3G48350.13.1e-11358.19Cysteine proteinases superfamily protein [more]
AT3G48340.13.4e-11256.65Cysteine proteinases superfamily protein [more]
AT5G50260.12.2e-11154.94Cysteine proteinases superfamily protein [more]
AT5G43060.15.4e-8649.69Granulin repeat cysteine protease family protein [more]
AT4G35350.11.1e-8348.37xylem cysteine peptidase 1 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000668Peptidase C1A, papain C-terminalPRINTSPR00705PAPAINcoord: 147..162
score: 65.62
coord: 303..309
score: 65.52
coord: 287..297
score: 50.32
IPR000668Peptidase C1A, papain C-terminalSMARTSM00645pept_c1coord: 129..343
e-value: 2.0E-109
score: 379.5
IPR000668Peptidase C1A, papain C-terminalPFAMPF00112Peptidase_C1coord: 129..342
e-value: 5.6E-76
score: 255.5
IPR013201Cathepsin propeptide inhibitor domain (I29)SMARTSM00848Inhibitor_I29_2coord: 40..95
e-value: 1.8E-10
score: 50.8
IPR013201Cathepsin propeptide inhibitor domain (I29)PFAMPF08246Inhibitor_I29coord: 40..95
e-value: 8.2E-11
score: 42.2
NoneNo IPR availableGENE3D3.90.70.10Cysteine proteinasescoord: 15..344
e-value: 1.0E-114
score: 385.6
NoneNo IPR availablePANTHERPTHR12411CYSTEINE PROTEASE FAMILY C1-RELATEDcoord: 20..344
NoneNo IPR availablePANTHERPTHR12411:SF706KDEL-TAILED CYSTEINE ENDOPEPTIDASE CEP1coord: 20..344
IPR000169Cysteine peptidase, cysteine active sitePROSITEPS00139THIOL_PROTEASE_CYScoord: 147..158
IPR025661Cysteine peptidase, asparagine active sitePROSITEPS00640THIOL_PROTEASE_ASNcoord: 303..322
IPR025660Cysteine peptidase, histidine active sitePROSITEPS00639THIOL_PROTEASE_HIScoord: 285..295
IPR039417Papain-like cysteine endopeptidaseCDDcd02248Peptidase_C1Acoord: 130..342
e-value: 1.0641E-86
score: 258.323
IPR038765Papain-like cysteine peptidase superfamilySUPERFAMILY54001Cysteine proteinasescoord: 32..343

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh12G000420.1CmaCh12G000420.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0051603 proteolysis involved in cellular protein catabolic process
biological_process GO:0006508 proteolysis
cellular_component GO:0005615 extracellular space
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005764 lysosome
molecular_function GO:0004197 cysteine-type endopeptidase activity
molecular_function GO:0008234 cysteine-type peptidase activity