CmaCh12G000300 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh12G000300
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	pectinesterase inhibitor 12-like
Location	Cma_Chr12: 113229 .. 114029 (+)
RNA-Seq Expression	CmaCh12G000300
Synteny	CmaCh12G000300

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGGATGCCAAGCAATCGCTACTCTGAAGGGTAAGACCCACGAAACCAAACTATCTGTTACAGTTTTAGACCAAACAAAACACACCAATCAAATTCAGTATATAAGACTACGTTCCTGTAATCAATGGCTCAAACCCATCTTTTCAATTCAAGGAATACCACCTCCCTGTGTCTTGGAATCCAGGCGCAGACACACACACACACACACACGCCTCGGATTGCAGGGATTGAGCAAGTCTTTTGCTTTTTAAGATTAAAAAAATAATAATAAATAAATAGTAAAAAGAATGGGTTTCCGAAGCCGTTTCTGGTGTGGTTGTTTGTTGTTGGTAGCCACGGCGGGTATGTGGCAGGGCGGGCAGGGGCAGGAGCGAGAGCTTCTAAGTCTGAATATGGGAGACCTGTGCGGAAAGGCAAACTACCCGGTTTTGTGTCGATCTATGGTGAAGGGTCTGAGTAACCCATCTGTAGCCTTAGAATCCGCCATCAGGCAGTTGATGTTGCAAACAAAAATGGCGAGGACTACAGCCAGGAGGCTGAGGAAGTCACCGGGCATGGAGGTATGCTCCGAGACTTACCAAGACGCCTTCAGCAACCTCAAGACATGCCTTTCAACCCTCAAAACCGGAGACAAACCTACCCTCAACATCAACCTCTCTGCAGCTTTGACCGACTACGTCACCTGCGACGATGCCGTGGCCGAGAGAGGCAGCACTTCTCCCGTAACCCGAACAAACCTTACACTCCGCCAAATGGCCACCAACTGTTTGTACTTGTCTAGTTTGATTGGCGTTCGCTGA

mRNA sequence

ATGGGATGCCAAGCAATCGCTACTCTGAAGGCCACGGCGGGTATGTGGCAGGGCGGGCAGGGGCAGGAGCGAGAGCTTCTAAGTCTGAATATGGGAGACCTGTGCGGAAAGGCAAACTACCCGGTTTTGTGTCGATCTATGGTGAAGGGTCTGAGTAACCCATCTGTAGCCTTAGAATCCGCCATCAGGCAGTTGATGTTGCAAACAAAAATGGCGAGGACTACAGCCAGGAGGCTGAGGAAGTCACCGGGCATGGAGGTATGCTCCGAGACTTACCAAGACGCCTTCAGCAACCTCAAGACATGCCTTTCAACCCTCAAAACCGGAGACAAACCTACCCTCAACATCAACCTCTCTGCAGCTTTGACCGACTACGTCACCTGCGACGATGCCGTGGCCGAGAGAGGCAGCACTTCTCCCGTAACCCGAACAAACCTTACACTCCGCCAAATGGCCACCAACTGTTTGTACTTGTCTAGTTTGATTGGCGTTCGCTGA

Coding sequence (CDS)

ATGGGATGCCAAGCAATCGCTACTCTGAAGGCCACGGCGGGTATGTGGCAGGGCGGGCAGGGGCAGGAGCGAGAGCTTCTAAGTCTGAATATGGGAGACCTGTGCGGAAAGGCAAACTACCCGGTTTTGTGTCGATCTATGGTGAAGGGTCTGAGTAACCCATCTGTAGCCTTAGAATCCGCCATCAGGCAGTTGATGTTGCAAACAAAAATGGCGAGGACTACAGCCAGGAGGCTGAGGAAGTCACCGGGCATGGAGGTATGCTCCGAGACTTACCAAGACGCCTTCAGCAACCTCAAGACATGCCTTTCAACCCTCAAAACCGGAGACAAACCTACCCTCAACATCAACCTCTCTGCAGCTTTGACCGACTACGTCACCTGCGACGATGCCGTGGCCGAGAGAGGCAGCACTTCTCCCGTAACCCGAACAAACCTTACACTCCGCCAAATGGCCACCAACTGTTTGTACTTGTCTAGTTTGATTGGCGTTCGCTGA

Protein sequence

MGCQAIATLKATAGMWQGGQGQERELLSLNMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQTKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTCDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR

Homology

[Close]

BLAST of CmaCh12G000300 vs. ExPASy Swiss-Prot
Match: Q8GT41 (Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 3.7e-05
Identity = 28/77 (36.36%), Postives = 44/77 (57.14%), Query Frame = 0


Query: 88  CSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTCDDAVAE-RGSTSPVTRTNL 147
           C   Y DA S+++  ++  K+ D  + N+ +SAAL D VTC+D   E +G  SPVT+ N 
Sbjct: 102 CVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIVSPVTKENK 161

Query: 148 TLRQMATNCLYLSSLIG 164
              Q+    L ++ L+G
Sbjct: 162 DYVQLTAISLAITKLLG 178

[Close]

BLAST of CmaCh12G000300 vs. ExPASy Swiss-Prot
Match: A9YUH4 (Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1)

HSP 1 Score: 49.7 bits (117), Expect = 3.7e-05
Identity = 28/77 (36.36%), Postives = 44/77 (57.14%), Query Frame = 0


Query: 88  CSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTCDDAVAE-RGSTSPVTRTNL 147
           C   Y DA S+++  ++  K+ D  + N+ +SAAL D VTC+D   E +G  SPVT+ N 
Sbjct: 93  CVGLYADAKSSVQEAIADFKSKDYASANVKMSAALDDSVTCEDGFKEKKGIASPVTKENK 152

Query: 148 TLRQMATNCLYLSSLIG 164
              Q+    L ++ L+G
Sbjct: 153 DYVQLTAISLAITKLLG 169

[Close]

BLAST of CmaCh12G000300 vs. ExPASy Swiss-Prot
Match: Q9FJR5 (Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1)

HSP 1 Score: 45.4 bits (106), Expect = 7.0e-04
Identity = 26/64 (40.62%), Postives = 35/64 (54.69%), Query Frame = 0


Query: 85  MEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTCDDAVAERGSTSPVTRT 144
           +++C   Y DA  +L T L+ +K+ D    NINLSAAL     C+D   E   TSP+T  
Sbjct: 97  LKLCLGFYNDANDDLTTALANIKSRDYQGANINLSAALDVPGNCEDDFKEAKKTSPITNE 156

Query: 145 NLTL 149
           N  L
Sbjct: 157 NSIL 160

[Close]

BLAST of CmaCh12G000300 vs. ExPASy TrEMBL
Match: A0A6J1KPW1 (pectinesterase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111496527 PE=4 SV=1)

HSP 1 Score: 303.9 bits (777), Expect = 4.1e-79
Identity = 156/157 (99.36%), Postives = 156/157 (99.36%), Query Frame = 0


Query: 9   LKATAGMWQGGQGQERELLSLNMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQ 68
           L ATAGMWQGGQGQERELLSLNMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQ
Sbjct: 14  LVATAGMWQGGQGQERELLSLNMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQ 73

Query: 69  TKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTC 128
           TKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTC
Sbjct: 74  TKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTC 133

Query: 129 DDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 166
           DDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR
Sbjct: 134 DDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 170

[Close]

BLAST of CmaCh12G000300 vs. ExPASy TrEMBL
Match: A0A6J1GK21 (pectinesterase inhibitor 12-like OS=Cucurbita moschata OX=3662 GN=LOC111454582 PE=4 SV=1)

HSP 1 Score: 270.4 bits (690), Expect = 5.0e-69
Identity = 140/156 (89.74%), Postives = 146/156 (93.59%), Query Frame = 0


Query: 13  AGMWQGGQGQERELLSL----NMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQ 72
           AGMW+GGQGQ RELLSL    NMGDLCGKA+YPVLCRS+VKG+S PSVALESAIRQLMLQ
Sbjct: 2   AGMWEGGQGQGRELLSLNLDPNMGDLCGKADYPVLCRSVVKGVSKPSVALESAIRQLMLQ 61

Query: 73  TKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTC 132
           TKMARTTA+RLRKSPG+EVCSETYQDAF NLKTCLSTLKTGDKPTLNINLSAALT YVTC
Sbjct: 62  TKMARTTAKRLRKSPGVEVCSETYQDAFRNLKTCLSTLKTGDKPTLNINLSAALTGYVTC 121

Query: 133 DDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGV 165
           DDAV ERG TSPVTRTNLTLRQMATNCLYLSSLIGV
Sbjct: 122 DDAVVERGGTSPVTRTNLTLRQMATNCLYLSSLIGV 157

[Close]

BLAST of CmaCh12G000300 vs. ExPASy TrEMBL
Match: A0A6J1BQY5 (pectinesterase inhibitor 12-like OS=Momordica charantia OX=3673 GN=LOC111004955 PE=4 SV=1)

HSP 1 Score: 183.0 bits (463), Expect = 1.0e-42
Identity = 103/165 (62.42%), Postives = 125/165 (75.76%), Query Frame = 0


Query: 13  AGMWQGG--QGQ----ERELL----SLNMG-DLCGKANYPVLCRSMVKGLSNPSVALESA 72
           AGMW GG  QGQ    ERELL    +LN+G D+C KA+YP +CRS+VKGL+NP VA E+A
Sbjct: 15  AGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSVVKGLNNPRVAFEAA 74

Query: 73  IRQLMLQTKMARTTARRLR-KSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSA 132
           +RQLM QT  A++ A+R + KS  MEVC E Y DA+SNL+T LS LK+ DK +LN++LSA
Sbjct: 75  VRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSA 134

Query: 133 ALTDYVTCDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 166
            LTDYVTCDDA+ E G TSPVTRTN  L QMA+NCLY SSLI +R
Sbjct: 135 VLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 179

[Close]

BLAST of CmaCh12G000300 vs. ExPASy TrEMBL
Match: A0A5A7V5S8 (Putative pectinesterase/pectinesterase inhibitor 40 OS=Cucumis melo var. makuwa OX=1194695 GN=E6C27_scaffold501G002070 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 2.0e-41
Identity = 94/155 (60.65%), Postives = 120/155 (77.42%), Query Frame = 0


Query: 9   LKATAGMWQGGQGQERELLSLNMG-DLCGKANYPVLCRSMVKGLSNPSVALESAIRQLML 68
           L   A +W     +    L+LN+  DLC KA+YP LCRS+VKGL++PS A+E++I++LM+
Sbjct: 19  LLVVAMLWSSPGSEAALTLNLNLSLDLCKKADYPALCRSVVKGLTDPSTAMETSIKKLMV 78

Query: 69  QTKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVT 128
           +TK A + A+R +KS  M+VC E Y DA+SNL+T LS+LK+ DK TLNINLSA+LTDYVT
Sbjct: 79  ETKQAMSVAKR-QKSSAMDVCIEVYDDAYSNLETSLSSLKSHDKGTLNINLSASLTDYVT 138

Query: 129 CDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLI 163
           CDDA+AERGSTSPVTR N  LR+M TNCLYLS LI
Sbjct: 139 CDDAIAERGSTSPVTRNNKLLREMTTNCLYLSGLI 172

[Close]

BLAST of CmaCh12G000300 vs. ExPASy TrEMBL
Match: A0A1S3BAX0 (uncharacterized protein LOC103487709 OS=Cucumis melo OX=3656 GN=LOC103487709 PE=4 SV=1)

HSP 1 Score: 178.7 bits (452), Expect = 2.0e-41
Identity = 94/155 (60.65%), Postives = 120/155 (77.42%), Query Frame = 0


Query: 9   LKATAGMWQGGQGQERELLSLNMG-DLCGKANYPVLCRSMVKGLSNPSVALESAIRQLML 68
           L   A +W     +    L+LN+  DLC KA+YP LCRS+VKGL++PS A+E++I++LM+
Sbjct: 19  LLVVAMLWSSPGSEAALTLNLNLSLDLCKKADYPALCRSVVKGLTDPSTAMETSIKKLMV 78

Query: 69  QTKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVT 128
           +TK A + A+R +KS  M+VC E Y DA+SNL+T LS+LK+ DK TLNINLSA+LTDYVT
Sbjct: 79  ETKQAMSVAKR-QKSSAMDVCIEVYDDAYSNLETSLSSLKSHDKGTLNINLSASLTDYVT 138

Query: 129 CDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLI 163
           CDDA+AERGSTSPVTR N  LR+M TNCLYLS LI
Sbjct: 139 CDDAIAERGSTSPVTRNNKLLREMTTNCLYLSGLI 172

[Close]

BLAST of CmaCh12G000300 vs. NCBI nr
Match: XP_023002765.1 (pectinesterase 3-like [Cucurbita maxima])

HSP 1 Score: 303.9 bits (777), Expect = 8.4e-79
Identity = 156/157 (99.36%), Postives = 156/157 (99.36%), Query Frame = 0


Query: 9   LKATAGMWQGGQGQERELLSLNMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQ 68
           L ATAGMWQGGQGQERELLSLNMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQ
Sbjct: 14  LVATAGMWQGGQGQERELLSLNMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQ 73

Query: 69  TKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTC 128
           TKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTC
Sbjct: 74  TKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTC 133

Query: 129 DDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 166
           DDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR
Sbjct: 134 DDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 170

[Close]

BLAST of CmaCh12G000300 vs. NCBI nr
Match: XP_023536926.1 (uncharacterized protein LOC111798158 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 274.2 bits (700), Expect = 7.1e-70
Identity = 146/165 (88.48%), Postives = 150/165 (90.91%), Query Frame = 0


Query: 9   LKATAGMWQGGQG----QERELLSL----NMGDLCGKANYPVLCRSMVKGLSNPSVALES 68
           L A AGMW+GGQG    QERELLSL    NMGDLCGKA YPVLCRS+VKGLS PSVALES
Sbjct: 14  LVAMAGMWEGGQGQGQEQERELLSLNLDPNMGDLCGKAEYPVLCRSVVKGLSKPSVALES 73

Query: 69  AIRQLMLQTKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSA 128
           AIRQLMLQTKMARTTA+RLRKSPGMEVCSETY+DAF NLKTCLSTLKTGDKPTLNINLSA
Sbjct: 74  AIRQLMLQTKMARTTAKRLRKSPGMEVCSETYKDAFGNLKTCLSTLKTGDKPTLNINLSA 133

Query: 129 ALTDYVTCDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 166
           ALT YVTCDDAVAERG TSPVTRTNLTLRQMATNCLYLSSLIGVR
Sbjct: 134 ALTGYVTCDDAVAERGGTSPVTRTNLTLRQMATNCLYLSSLIGVR 178

[Close]

BLAST of CmaCh12G000300 vs. NCBI nr
Match: KAG6585173.1 (hypothetical protein SDJN03_17906, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 273.1 bits (697), Expect = 1.6e-69
Identity = 144/159 (90.57%), Postives = 148/159 (93.08%), Query Frame = 0


Query: 13  AGMWQGGQG--QERELLSL----NMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLM 72
           AGMW+GGQG  QERELLSL    NMGDLCGKA+YPVLCRS+VKGLS PSVALESAIRQLM
Sbjct: 2   AGMWEGGQGQEQERELLSLNLYPNMGDLCGKADYPVLCRSVVKGLSRPSVALESAIRQLM 61

Query: 73  LQTKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYV 132
           LQTKMARTTA+RLRKSPGMEVCSETYQDAF NLKTCLSTLKTGDKPTLNINLSAALT YV
Sbjct: 62  LQTKMARTTAKRLRKSPGMEVCSETYQDAFRNLKTCLSTLKTGDKPTLNINLSAALTGYV 121

Query: 133 TCDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 166
           TCDDAV ERG TSPVTRTNLTLRQMATNCLYLSSLIGVR
Sbjct: 122 TCDDAVVERGGTSPVTRTNLTLRQMATNCLYLSSLIGVR 160

[Close]

BLAST of CmaCh12G000300 vs. NCBI nr
Match: XP_022951850.1 (pectinesterase inhibitor 12-like [Cucurbita moschata])

HSP 1 Score: 270.4 bits (690), Expect = 1.0e-68
Identity = 140/156 (89.74%), Postives = 146/156 (93.59%), Query Frame = 0


Query: 13  AGMWQGGQGQERELLSL----NMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQ 72
           AGMW+GGQGQ RELLSL    NMGDLCGKA+YPVLCRS+VKG+S PSVALESAIRQLMLQ
Sbjct: 2   AGMWEGGQGQGRELLSLNLDPNMGDLCGKADYPVLCRSVVKGVSKPSVALESAIRQLMLQ 61

Query: 73  TKMARTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTC 132
           TKMARTTA+RLRKSPG+EVCSETYQDAF NLKTCLSTLKTGDKPTLNINLSAALT YVTC
Sbjct: 62  TKMARTTAKRLRKSPGVEVCSETYQDAFRNLKTCLSTLKTGDKPTLNINLSAALTGYVTC 121

Query: 133 DDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGV 165
           DDAV ERG TSPVTRTNLTLRQMATNCLYLSSLIGV
Sbjct: 122 DDAVVERGGTSPVTRTNLTLRQMATNCLYLSSLIGV 157

[Close]

BLAST of CmaCh12G000300 vs. NCBI nr
Match: XP_022131971.1 (pectinesterase inhibitor 12-like [Momordica charantia])

HSP 1 Score: 183.0 bits (463), Expect = 2.2e-42
Identity = 103/165 (62.42%), Postives = 125/165 (75.76%), Query Frame = 0


Query: 13  AGMWQGG--QGQ----ERELL----SLNMG-DLCGKANYPVLCRSMVKGLSNPSVALESA 72
           AGMW GG  QGQ    ERELL    +LN+G D+C KA+YP +CRS+VKGL+NP VA E+A
Sbjct: 15  AGMWYGGHAQGQSQERERELLGLSINLNLGFDICRKADYPAMCRSVVKGLNNPRVAFEAA 74

Query: 73  IRQLMLQTKMARTTARRLR-KSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSA 132
           +RQLM QT  A++ A+R + KS  MEVC E Y DA+SNL+T LS LK+ DK +LN++LSA
Sbjct: 75  VRQLMFQTSRAKSVAQRNKDKSSAMEVCFEVYDDAYSNLETGLSNLKSHDKSSLNVDLSA 134

Query: 133 ALTDYVTCDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLIGVR 166
            LTDYVTCDDA+ E G TSPVTRTN  L QMA+NCLY SSLI +R
Sbjct: 135 VLTDYVTCDDAINEEGITSPVTRTNAILSQMASNCLYFSSLIHLR 179

[Close]

BLAST of CmaCh12G000300 vs. TAIR 10
Match: AT1G23350.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 104.8 bits (260), Expect = 7.0e-23
Identity = 55/139 (39.57%), Postives = 89/139 (64.03%), Query Frame = 0


Query: 25  ELLSLNMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQTKMARTTA-RRLRKSP 84
           + LS+++ D C  +++P LCRS +KG +N + A + AI++LM +T+ A+  A + L++  
Sbjct: 19  QALSISLADPCTVSDFPALCRSTIKGQTNVNAATDVAIKELMKRTRQAKKIAEKELKRDG 78

Query: 85  GMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTCDDAVAERGSTSPVTR 144
           G+  C   +  AF NL+  L+ +K  D  +LNINLSAALTDY TC D++ E    + + +
Sbjct: 79  GVATCLSNFNSAFDNLEKALTNIKENDGFSLNINLSAALTDYDTCSDSMKETKEVNVIYK 138

Query: 145 TNLTLRQMATNCLYLSSLI 163
           +   L +MA NCL LS+L+
Sbjct: 139 SAGVLYRMADNCLALSTLV 157

[Close]

BLAST of CmaCh12G000300 vs. TAIR 10
Match: AT1G70540.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 99.8 bits (247), Expect = 2.2e-21
Identity = 56/131 (42.75%), Postives = 83/131 (63.36%), Query Frame = 0


Query: 33  DLCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQTKMARTTARRLRKS-PGMEVCSET 92
           D C  +++ VLCRS+VKG  N + A E +IR+LM +T  A+  A+  RKS  G++ C   
Sbjct: 35  DPCSVSDFKVLCRSVVKGQKNVNAATEVSIRELMKRTIKAKEAAKISRKSGGGLKTCYSN 94

Query: 93  YQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTCDDAVAERGSTSPVTRTNLTLRQM 152
           Y  A  NL+  L  +K  D  +LNINLSA+LTD+ TC+DA+    +++   ++  TL +M
Sbjct: 95  YDSALENLQKALKNIKQNDGFSLNINLSASLTDFDTCNDAMGGGTASNVFAKSTSTLHEM 154

Query: 153 ATNCLYLSSLI 163
           A NCL LS+L+
Sbjct: 155 ADNCLALSTLV 165

[Close]

BLAST of CmaCh12G000300 vs. TAIR 10
Match: AT3G36659.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 82.4 bits (202), Expect = 3.7e-16
Identity = 54/143 (37.76%), Postives = 75/143 (52.45%), Query Frame = 0


Query: 26  LLSLNMGDLCGKANYPVLCRSMVKGLSNP------SVALESAIRQLMLQTKMARTTARRL 85
           LLS  +  +CGK + P LC S V  L  P      S  L  AI+  +  TK A     ++
Sbjct: 125 LLSPEIKTICGKTDNPPLCESSVSPLLTPQLKPNTSSVLILAIQASITATKAAMAIVEKV 184

Query: 86  RKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTCDDAVAERGSTS 145
             S     C E Y DA  NL+  ++ +K+ D  T+N NLSAA+TDY TC+D   E G  +
Sbjct: 185 DASD----CQELYDDAVVNLEDAVNAVKSSDIATVNTNLSAAMTDYSTCNDGFEESGEPN 244

Query: 146 PVTRTNLTLRQMATNCLYLSSLI 163
           P+      L +M +NCL +S+LI
Sbjct: 245 PLAYVADKLTKMVSNCLAISTLI 263

[Close]

BLAST of CmaCh12G000300 vs. TAIR 10
Match: AT1G10770.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 78.2 bits (191), Expect = 7.0e-15
Identity = 51/159 (32.08%), Postives = 81/159 (50.94%), Query Frame = 0


Query: 5   AIATLKATAGMWQGGQGQERELLSLNMGDLCGKANYPVLCRSMVKGLSNPSVALESAIRQ 64
           A+A   A       GQ  E  ++     ++C    YP LCR +VK +++P  A    I+ 
Sbjct: 13  AVAASAAVLIFLMAGQVAESRMI-----NICSHTAYPSLCRPLVKRVTSPRKATHRTIQA 72

Query: 65  LMLQTKMA-RTTARRLRKSPGMEVCSETYQDAFSNLKTCLSTLKTGDKPTLNINLSAALT 124
           L  +TK+A   TAR    +  +  C ET  DA  NL +   +++  D P +N  L+AA++
Sbjct: 73  LEAKTKLALAETARFKNGNQAVSTCYETLGDAVYNLASARKSIRKRDVPAMNTYLTAAVS 132

Query: 125 DYVTCDDAVAERGSTSPVTRTNLTLRQMATNCLYLSSLI 163
           DY  C D   E    + +    + LR++++NCL LS+LI
Sbjct: 133 DYGACVDGFIETQQVNAIQNAVVDLRKISSNCLALSTLI 166

[Close]

BLAST of CmaCh12G000300 vs. TAIR 10
Match: AT1G60760.1 (Plant invertase/pectin methylesterase inhibitor superfamily protein )

HSP 1 Score: 72.4 bits (176), Expect = 3.8e-13
Identity = 46/130 (35.38%), Postives = 72/130 (55.38%), Query Frame = 0


Query: 34  LCGKANYPVLCRSMVKGLSNPSVALESAIRQLMLQTKMARTTARRLRK-SPGMEVCSETY 93
           LC +  YP LCR +VKG  NP  A  S IR L  +TK+A T + R +  +P + +C  T 
Sbjct: 34  LCAQTAYPTLCRPLVKG-PNPRRATHSTIRALEAKTKLAITDSARFKSGNPTVAICYATL 93

Query: 94  QDAFSNLKTCLSTLKTGDKPTLNINLSAALTDYVTCDDAVAERGSTSPVTRTNLTLRQMA 153
            DA  NL+   ++++  +   L + L+AA++DY  C +   +    + +      LR+M 
Sbjct: 94  VDASFNLRRARTSIRKRNVMMLKMFLTAAVSDYGVCVNGFIDSHQVNTLQNDVDELRKMG 153

Query: 154 TNCLYLSSLI 163
           TNCL L++LI
Sbjct: 154 TNCLLLATLI 162

The following BLAST results are available for this feature:

BLAST of CmaCh12G000300 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q8GT41	3.7e-05	36.36	Putative invertase inhibitor OS=Platanus acerifolia OX=140101 PE=1 SV=1	[more]
A9YUH4	3.7e-05	36.36	Putative invertase inhibitor OS=Platanus orientalis OX=122832 PE=1 SV=1	[more]
Q9FJR5	7.0e-04	40.63	Pectinesterase inhibitor 12 OS=Arabidopsis thaliana OX=3702 GN=PMEI12 PE=2 SV=1	[more]

BLAST of CmaCh12G000300 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1KPW1	4.1e-79	99.36	pectinesterase 3-like OS=Cucurbita maxima OX=3661 GN=LOC111496527 PE=4 SV=1	[more]
A0A6J1GK21	5.0e-69	89.74	pectinesterase inhibitor 12-like OS=Cucurbita moschata OX=3662 GN=LOC111454582 P...	[more]
A0A6J1BQY5	1.0e-42	62.42	pectinesterase inhibitor 12-like OS=Momordica charantia OX=3673 GN=LOC111004955 ...	[more]
A0A5A7V5S8	2.0e-41	60.65	Putative pectinesterase/pectinesterase inhibitor 40 OS=Cucumis melo var. makuwa ...	[more]
A0A1S3BAX0	2.0e-41	60.65	uncharacterized protein LOC103487709 OS=Cucumis melo OX=3656 GN=LOC103487709 PE=...	[more]

BLAST of CmaCh12G000300 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_023002765.1	8.4e-79	99.36	pectinesterase 3-like [Cucurbita maxima]	[more]
XP_023536926.1	7.1e-70	88.48	uncharacterized protein LOC111798158 [Cucurbita pepo subsp. pepo]	[more]
KAG6585173.1	1.6e-69	90.57	hypothetical protein SDJN03_17906, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022951850.1	1.0e-68	89.74	pectinesterase inhibitor 12-like [Cucurbita moschata]	[more]
XP_022131971.1	2.2e-42	62.42	pectinesterase inhibitor 12-like [Momordica charantia]	[more]

BLAST of CmaCh12G000300 vs. TAIR 10


Match Name	E-value	Identity	Description
AT1G23350.1	7.0e-23	39.57	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G70540.1	2.2e-21	42.75	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT3G36659.1	3.7e-16	37.76	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G10770.1	7.0e-15	32.08	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]
AT1G60760.1	3.8e-13	35.38	Plant invertase/pectin methylesterase inhibitor superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 26..158 e-value: 6.3E-21 score: 85.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 33..157 e-value: 6.1E-17 score: 62.3
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 59..160 e-value: 1.2E-14 score: 52.0
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 32..163 e-value: 1.4E-19 score: 72.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 32..162
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 30..162
None	No IPR available	PANTHER	PTHR31707:SF270	PLANT INVERTASE/PECTIN METHYLESTERASE INHIBITOR SUPERFAMILY PROTEIN	coord: 30..162
None	No IPR available	CDD	cd15800	PMEI-like_2	coord: 33..162 e-value: 1.12273E-45 score: 144.81

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh12G000300.1	CmaCh12G000300.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0043086	negative regulation of catalytic activity
molecular_function	GO:0004857	enzyme inhibitor activity