CmaCh11G019180 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh11G019180
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionmitotic checkpoint serine/threonine-protein kinase BUB1
LocationCma_Chr11: 12659749 .. 12663917 (+)
RNA-Seq ExpressionCmaCh11G019180
SyntenyCmaCh11G019180
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ACAGTCTGGGTCGGCGTCGTTCACTTCCGTTTCTTCCACGAGCAGTAAGCTACTGGCCATGGCGATAATCTTCCCTGATTCTCAAATCACCAGCACTACAACATCTGTTCAGGACCCTTTGTTACCTTGGCTCTGGTATTCCTTCATTTCTTTTCTGTAATCGAGCTGATTCACAATCATTCTTCCATCCAGTTTCAGTGAAATTGTTAATGCACATTTGGTTCCAATCGCAGGTCCATAAAGGATGCTCTTGAAAATTCGACCTCCGGCAATGTTTCTGGGCCAGGTCTCGCGGATCTCGTTTCTGACTGTATTAAAACCTTCGGCAGCACTGTCAAGTACAAAAACGATGTCCGATTTCTCAAGATTTGGTTTCTCTCTGTGCGTAACTATCTTTCTTTCACTGTTATATCTGTGGGTTTAAAAGTTTAATCATCTTTGTTTCGTAGATGCAGTTGATTAGATAACTTTGACAGAGTTTTAATAATTTCGGTTTATTGATTACGAATAATAATTAAGATTATATTGTTCGTGTAAGTAGATTGGTATGAGGGAGGATTTTGAAACCGGTTTCAAGGAATTATTGGAGCAGGAGATATGCATTGGCAATTCTTTGCTTTATATTTGGTATGCAGTGTTCCTTGAATCCAAGGGGAGACTAAACGACGCTAACATGATTTATGAGATTGGCTTGTTGAGGTATCTCTATCTCCCTCTCATGGGCATACTGCTGAATACATGTACGATTATGCTTGTTCCTTACATTTTTTAACTGATAACAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAATGTTGTTTATTGATAGGATGTCTGAGTTAGTCAATACTCATTCAATGAAATTGGTGCGTGTTCTTAAAGCACAGAGCAAATGAGATTATGTTCCTTCAAGTATATAACGTAGGAATTTTGACTTATAAATTTCGTTTTTGTAGAATGACGGTGGTGAATCCACTAAATTTGTAGAAACTTACGTGAATCCATGGTCTTCCTCTGTTATGAAAGATTTATTAAGCAAGATTAATCTCAAGATTTTGAAGTATGAGGTAAGAAAGTGCAGCTTTCCTTGTCCAATGATGTAAGCAAGCTAGTTATTACTCTCTTACTAACTTGACTATGAATCTAATGCATGTTTCAGGGTTACCATTCAAGCATTAAACCTTACCCTAAAAAGATGGCTCTAAATTCATTGCGGAATTCATCCAGGAATAAAGTTATTGAGATTGGTAATGGCATAGTTAGATTTTTCACCTAAATAGATAGAACTACCTTCAGTCAATCCTTCAAAACAGCGAGATTTCTCGAAATATTTTGTAAGAAATGAAGGAAAAAATGTTCTTATTTCATTCATACTCTATTCATCATGATCGTTCTAATTCAGTTGCCAAAATTTTCAGGTGGGAAGAAATATGAGATCAAAGGTTGTGCAGGAACTGGCGGCTTTGCCCAAGTATATAAATCATATCTTGACAGTAACCCAGATGATGTTGTCGCACTAAAGGTAGAAAATCTGCATATACCCTTTACTTTAAGCATTCATAATACGCCATACCTGAATGTGAGCTTGCTGGATGTCCTGATTCCCTTCAAATGCTTGTCAGATACAAACACCTCCTTTTCCTTGGGAATTCTACATTTATCGTCAACTTGATCAACGTGTCACCGTGAAGGAAGTATGTTGTAGCCCTTTAGATTTTCAGTAAGCGCTTATTACTTCTGAATGTTAGCAACTCGATTTATGCTCATCCTCAATTTCTCACAATGTCAGAGGTCAAGTTTTGGTTGTGCTCATGAGATGTATCTCTATTCTGACTATAGCATACTTATTTGTGACTACTTGGCTCATGGCACATTACAGGTGAATTTTCAGATTCTGAACCTTTCTGTACTGTATGGTCCTTTAAAATACTCACCGTAAGTAACTGTGTGGTTAAAACCTGGTGCAGGATGCCATTAATTTATATGTAGTCCTTGGGAAGACAATGGAAGAAGTTTTGTGTATTTATTACACCATAGAAATGCTTTACATGCTAGAGACTTTGCACAACATTGGCATTATCCATGGGGATTTCAAACCTGACAATCTGCTTATTCGTTATGCAAGGTAAAAGTTGATTTCATTAAATAGTTCACTCTAGTTTTCATTTGCAACTTTGGCCCACAAATAATGATCCGCTTCGGTGGGATCCCATATCGGTTGAAAAGGAGAATGAAGCATTTTTTATAAGGGTGTGGAAACCTCACGTTTTAGAAACTTTGAGGAGAAGCTCGAAAGGAAAAGTCCAAAGAGAACATTATCTGCTAACAGTGGACTTGGGCTATTACAAATGGTATTAGACCAGGACACCGGACGATATGTCAGTGAGAAGGCTGAGTCTTGAAAGGGGTGGACACGAGGCAGTGTGCCAGCAAGAACGTTGGACCCCAAAGGGGCGTGGATTGGGGGTCCCACATAGATTGGAGAAGGGAACGAGTACTGGCGAGGACGCCGAGCCCCGAAGGGGGTAGATTGTGAGATCCCACATCGGTTGAGGAGGAGAACAAAACATTATTTATAAGGATGTGAAAACCTCTCCCTAGCAGACGCGTTTTAAAAACCTTGAGGAAAGTCCGAATATACAATATCTGCTTGCTAGCGATGGGCTTAAGCTGTTCCAGAATCCGACTGAAAAATTTTCGTACTCCCATTCTATAATTCTCTACCAATTGACTTTAGTTTTGTTAACCATTATGCAGAGGCAAACTAACGGAAGATGGATTTCGGGAACGTAGTGGTCCTTGGAATGATCAGGTATGTAGGTTCATTATTCCTTGTTTTTGTTCCCAGTACATTACATTAGCTGAGAGCTAGGAAGATTACCTGTAGCTTTATCTTGTAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATCTGCAACTTTTTCCTGAAAATGTAGAATTTGAAGGAGATTGCCGAACTTCTGGCTTTCGTTGTACAGAAATGCTAGAGAATAAACCTTGGAAATATCAGGTCAGTCTCTTCTGCAGTGTGGTTATTTATAACAACTCTAGTGCTGCAAACCCATTGTGAAGAGGAAAACAAAAGTTCTATCAGTAGCATTCTGTGTTTGTACAGGTAGACATATATGGCTTCTGTGTGGTCGTCCACACTATGCTTCATGGTTCCTACATGGACGTTGAGAAAAAAGTATCATCTGATGGGAGCTTTGTTTATTTACCCAAAACTTCTTTTAAACGGTTAGTAACTTTTCTGGCGTCTTTACTCTGTTTCCTTTTCCTGTCCTGTGCAAAATACTTAGCCGTCACGCTTTTCTTTACATTATTAGTTGTTGCACATCAATTGCTACATCAATTATGTGAATAATGGAAATGATATTACGTATGCCTTTCATTTTTAAGCATTAGCCGTCACCTCAGTGTCCATCTTAGTTTGACGAAGAAGCTCTTCAAAGATCTTTTTGGAACAAATTTTCGTAAGGGTAGAAAAGAAACCTTTTGAAACTTATCCGTAGATAGATTCTTTGTAGAGTTTGAGTTTGAGTTTGAGTTTGAGTGAGCAACCAACCATCCTCTTCCCTTCCTCTCTCTGACTTGAACTCTCTGTTGTTCCGGTATAGGTATTGGAACGTTGAACTGTGGAAGAATCTATTCACGAAGTTGCTTAATATCAAGCTAGGTGATTACAACAAGGAGTTGTTGCAGAGTATAAGGGGATCTTTCCAGGATTACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTAGCAAAACAGAGGGCTTCCTTGTGTACCGCTTAGTCAAGAATCCATCTCCTCTGGAAGATAGATAGATGTAAATTCGGTCAGCTACAGGCCCCTTGCGTCTTGTTTGTACCAAAAATGTAAATTGGTTCATGTTTGCCACTTGCAAACCTGGCCTAATCCATTTGTTTTGGTGATGAAATCTTGGTTTTTTCTCGTCGGTTTCAAACACAAAAATGTTTGAATAATTGTAATATATAAGGGGTCTTCTCTGCATTGCTGTTTTGTTGTAGGCTTCATTGTTGATCAAGAGTTTCTTACTGTAATGTTGCGGGATGCAGATGTTCATGTAGATTATATATTGTTCGACTATTTTTAATATCTTTA

mRNA sequence

ACAGTCTGGGTCGGCGTCGTTCACTTCCGTTTCTTCCACGAGCAGTAAGCTACTGGCCATGGCGATAATCTTCCCTGATTCTCAAATCACCAGCACTACAACATCTGTTCAGGACCCTTTGTTACCTTGGCTCTGGTCCATAAAGGATGCTCTTGAAAATTCGACCTCCGGCAATGTTTCTGGGCCAGGTCTCGCGGATCTCGTTTCTGACTGTATTAAAACCTTCGGCAGCACTGTCAAGTACAAAAACGATGTCCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACCGGTTTCAAGGAATTATTGGAGCAGGAGATATGCATTGGCAATTCTTTGCTTTATATTTGGTATGCAGTGTTCCTTGAATCCAAGGGGAGACTAAACGACGCTAACATGATTTATGAGATTGGCTTGTTGAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAATGTTGTTTATTGATAGGATGTCTGAGTTAGTCAATACTCATTCAATGAAATTGAATGACGGTGGTGAATCCACTAAATTTGTAGAAACTTACGTGAATCCATGGTCTTCCTCTGTTATGAAAGATTTATTAAGCAAGATTAATCTCAAGATTTTGAAGTATGAGGGTTACCATTCAAGCATTAAACCTTACCCTAAAAAGATGGCTCTAAATTCATTGCGGAATTCATCCAGGAATAAAGTTATTGAGATTGGTGGGAAGAAATATGAGATCAAAGGTTGTGCAGGAACTGGCGGCTTTGCCCAAGTATATAAATCATATCTTGACAGTAACCCAGATGATGTTGTCGCACTAAAGATACAAACACCTCCTTTTCCTTGGGAATTCTACATTTATCGTCAACTTGATCAACGTGTCACCGTGAAGGAAAGGTCAAGTTTTGGTTGTGCTCATGAGATGTATCTCTATTCTGACTATAGCATACTTATTTGTGACTACTTGGCTCATGGCACATTACAGGATGCCATTAATTTATATGTAGTCCTTGGGAAGACAATGGAAGAAGTTTTGTGTATTTATTACACCATAGAAATGCTTTACATGCTAGAGACTTTGCACAACATTGGCATTATCCATGGGGATTTCAAACCTGACAATCTGCTTATTCGTTATGCAAGAGGCAAACTAACGGAAGATGGATTTCGGGAACGTAGTGGTCCTTGGAATGATCAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATCTGCAACTTTTTCCTGAAAATGTAGAATTTGAAGGAGATTGCCGAACTTCTGGCTTTCGTTGTACAGAAATGCTAGAGAATAAACCTTGGAAATATCAGGTAGACATATATGGCTTCTGTGTGGTCGTCCACACTATGCTTCATGGTTCCTACATGGACGTTGAGAAAAAAGTATCATCTGATGGGAGCTTTGTTTATTTACCCAAAACTTCTTTTAAACGGTATTGGAACGTTGAACTGTGGAAGAATCTATTCACGAAGTTGCTTAATATCAAGCTAGGTGATTACAACAAGGAGTTGTTGCAGAGTATAAGGGGATCTTTCCAGGATTACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTAGCAAAACAGAGGGCTTCCTTGTGTACCGCTTAGTCAAGAATCCATCTCCTCTGGAAGATAGATAGATGTAAATTCGGTCAGCTACAGGCCCCTTGCGTCTTGTTTGTACCAAAAATGTAAATTGGTTCATGTTTGCCACTTGCAAACCTGGCCTAATCCATTTGTTTTGGTGATGAAATCTTGGTTTTTTCTCGTCGGTTTCAAACACAAAAATGTTTGAATAATTGTAATATATAAGGGGTCTTCTCTGCATTGCTGTTTTGTTGTAGGCTTCATTGTTGATCAAGAGTTTCTTACTGTAATGTTGCGGGATGCAGATGTTCATGTAGATTATATATTGTTCGACTATTTTTAATATCTTTA

Coding sequence (CDS)

ATGGCGATAATCTTCCCTGATTCTCAAATCACCAGCACTACAACATCTGTTCAGGACCCTTTGTTACCTTGGCTCTGGTCCATAAAGGATGCTCTTGAAAATTCGACCTCCGGCAATGTTTCTGGGCCAGGTCTCGCGGATCTCGTTTCTGACTGTATTAAAACCTTCGGCAGCACTGTCAAGTACAAAAACGATGTCCGATTTCTCAAGATTTGGTTTCTCTCTATTGGTATGAGGGAGGATTTTGAAACCGGTTTCAAGGAATTATTGGAGCAGGAGATATGCATTGGCAATTCTTTGCTTTATATTTGGTATGCAGTGTTCCTTGAATCCAAGGGGAGACTAAACGACGCTAACATGATTTATGAGATTGGCTTGTTGAGGAACGCTGAGCCCCTTGAGTGGCTGAAAAAGGCAAAAATGTTGTTTATTGATAGGATGTCTGAGTTAGTCAATACTCATTCAATGAAATTGAATGACGGTGGTGAATCCACTAAATTTGTAGAAACTTACGTGAATCCATGGTCTTCCTCTGTTATGAAAGATTTATTAAGCAAGATTAATCTCAAGATTTTGAAGTATGAGGGTTACCATTCAAGCATTAAACCTTACCCTAAAAAGATGGCTCTAAATTCATTGCGGAATTCATCCAGGAATAAAGTTATTGAGATTGGTGGGAAGAAATATGAGATCAAAGGTTGTGCAGGAACTGGCGGCTTTGCCCAAGTATATAAATCATATCTTGACAGTAACCCAGATGATGTTGTCGCACTAAAGATACAAACACCTCCTTTTCCTTGGGAATTCTACATTTATCGTCAACTTGATCAACGTGTCACCGTGAAGGAAAGGTCAAGTTTTGGTTGTGCTCATGAGATGTATCTCTATTCTGACTATAGCATACTTATTTGTGACTACTTGGCTCATGGCACATTACAGGATGCCATTAATTTATATGTAGTCCTTGGGAAGACAATGGAAGAAGTTTTGTGTATTTATTACACCATAGAAATGCTTTACATGCTAGAGACTTTGCACAACATTGGCATTATCCATGGGGATTTCAAACCTGACAATCTGCTTATTCGTTATGCAAGAGGCAAACTAACGGAAGATGGATTTCGGGAACGTAGTGGTCCTTGGAATGATCAGGGGCTTTGCCTTGTTGATTGGGGAAGAGGTATAGATCTGCAACTTTTTCCTGAAAATGTAGAATTTGAAGGAGATTGCCGAACTTCTGGCTTTCGTTGTACAGAAATGCTAGAGAATAAACCTTGGAAATATCAGGTAGACATATATGGCTTCTGTGTGGTCGTCCACACTATGCTTCATGGTTCCTACATGGACGTTGAGAAAAAAGTATCATCTGATGGGAGCTTTGTTTATTTACCCAAAACTTCTTTTAAACGGTATTGGAACGTTGAACTGTGGAAGAATCTATTCACGAAGTTGCTTAATATCAAGCTAGGTGATTACAACAAGGAGTTGTTGCAGAGTATAAGGGGATCTTTCCAGGATTACATGTGCTCCAACCCTCAGCTTATAAGGAAGTTAAAGGAATTACTAGCAAAACAGAGGGCTTCCTTGTGTACCGCTTAG

Protein sequence

MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTVKYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVMKDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNLFTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA
Homology
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BLAST of CmaCh11G019180 vs. ExPASy Swiss-Prot
Match: F4IVI0 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana OX=3702 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 670.6 bits (1729), Expect = 1.4e-191
Identity = 308/515 (59.81%), Postives = 399/515 (77.48%), Query Frame = 0 

Query: 19  DPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTVKYKNDVRFLKIWFLSIGM 78
           DPL PWL  IK+++E+  +G  SG  L  L+ DCI T+    +Y+ND+RFLKIWFL +  
Sbjct: 11  DPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLYLEG 70

Query: 79  REDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYEIGLLRNAEPLEWLKK 138
           REDFE  ++E+ E EIC G+SLLY WYA+FLE KG    AN +Y+ GL R AEP + LK+
Sbjct: 71  REDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDRLKE 130

Query: 139 AKMLFIDRMSELVNTHSM-KLNDGGESTKFVETYVNPWSSSVMKDLLSKINLKILKYEGY 198
           A  LF+ R+S+     S+ K+ D  ++T     +VNPW +S +  L+ KI  +++KY+GY
Sbjct: 131 AHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKYDGY 190

Query: 199 HSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVA 258
           H S K +P K  L+SL+N SRNK+IEIGG+KY++KGCAG GGFAQV+K+++DSNPD+VVA
Sbjct: 191 HVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDEVVA 250

Query: 259 LKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYSILICDYLAHGTLQDAIN 318
           LK+Q PPFPWEF++YRQLD R+   +RSSFG A  +++YSDYSIL+CDYL+HGTLQD IN
Sbjct: 251 LKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQDVIN 310

Query: 319 LYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNLLIRYARGKLTEDGFRER 378
            YVV+GK+MEEVLC+YYTIEML MLETLH++GIIHGDFKPDNLLIRY    LTE GF E+
Sbjct: 311 SYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGFHEK 370

Query: 379 SGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEMLENKPWKYQVDIYGFCV 438
           +G W+ +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E+KPWK+QVD YG CV
Sbjct: 371 TGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYGLCV 430

Query: 439 VVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNLFTKLLNIKLGDYNKELL 498
           +VH MLH  YM++EKK S DG ++ +P+TSFKRYW V+LWK LFTKLLN +  + + E L
Sbjct: 431 IVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDTETL 490

Query: 499 QSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           +++R S ++Y+CS+P+L++KL ELLAKQR SLC++
Sbjct: 491 RNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

[Close]
BLAST of CmaCh11G019180 vs. ExPASy Swiss-Prot
Match: O43683 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 GN=BUB1 PE=1 SV=1)

HSP 1 Score: 191.0 bits (484), Expect = 3.3e-47
Identity = 113/321 (35.20%), Postives = 167/321 (52.02%), Query Frame = 0 

Query: 173  NPWSSSVMKDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKV-IEIGGKKYEI 232
            NPW   ++  LLS          G    +  YP          + + K   ++G K   +
Sbjct: 740  NPWDDKLIFKLLS----------GLSKPVSSYPNTFEWQCKLPAIKPKTEFQLGSKLVYV 799

Query: 233  KGCAGTGGFAQVYKS----YLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSF 292
                G G FAQVY++      D+       LK+Q P  PWEFYI  QL +R+    +  F
Sbjct: 800  HHLLGEGAFAQVYEATQGDLNDAKNKQKFVLKVQKPANPWEFYIGTQLMERLKPSMQHMF 859

Query: 293  GCAHEMYLYSDYSILICDYLAHGTLQDAINLYV-VLGKTMEEVLCIYYTIEMLYMLETLH 352
               +  +L+ + S+L+ +  ++GTL +AINLY     K M + L I + + MLYM+E +H
Sbjct: 860  MKFYSAHLFQNGSVLVGELYSYGTLLNAINLYKNTPEKVMPQGLVISFAMRMLYMIEQVH 919

Query: 353  NIGIIHGDFKPDNLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEF 412
            +  IIHGD KPDN ++    G L +D   + S      GL L+D G+ ID++LFP+   F
Sbjct: 920  DCEIIHGDIKPDNFIL--GNGFLEQDDEDDLSA-----GLALIDLGQSIDMKLFPKGTIF 979

Query: 413  EGDCRTSGFRCTEMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKT 472
               C TSGF+C EML NKPW YQ+D +G    V+ ML G+YM    KV ++G     P+ 
Sbjct: 980  TAKCETSGFQCVEMLSNKPWNYQIDYFGVAATVYCMLFGTYM----KVKNEGG-ECKPEG 1038

Query: 473  SFKRYWNVELWKNLFTKLLNI 488
             F+R  ++++W   F  +LNI
Sbjct: 1040 LFRRLPHLDMWNEFFHVMLNI 1038

[Close]
BLAST of CmaCh11G019180 vs. ExPASy Swiss-Prot
Match: O08901 (Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090 GN=Bub1 PE=1 SV=1)

HSP 1 Score: 190.7 bits (483), Expect = 4.4e-47
Identity = 108/323 (33.44%), Postives = 173/323 (53.56%), Query Frame = 0 

Query: 170  TYVNPWSSSVMKDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKY 229
            T  NPW   ++  LLS ++  +  Y         +  +  L +++  +     ++G    
Sbjct: 711  TVENPWDDELILKLLSGLSKPVTSYS------NTFEWQSKLPAIKTKTE---YQLGSLLV 770

Query: 230  EIKGCAGTGGFAQVYKS----YLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERS 289
             +    G G FAQV+++      ++  +    LK+Q P   WEFYI  QL +R+  +   
Sbjct: 771  YVNHLLGEGAFAQVFEAIHGDVRNAKSEQKCILKVQRPANSWEFYIGMQLMERLKPEVHH 830

Query: 290  SFGCAHEMYLYSDYSILICDYLAHGTLQDAINLYV-VLGKTMEEVLCIYYTIEMLYMLET 349
             F   +  +L+ + SIL+ +  ++GTL + INLY     K M + L + + I MLYM+E 
Sbjct: 831  MFIKFYSAHLFKNGSILVGELYSYGTLLNVINLYKNTSEKVMPQALVLTFAIRMLYMVEQ 890

Query: 350  LHNIGIIHGDFKPDNLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENV 409
            +H+  IIHGD KPDN ++ +         F E++      GL L+D G+ ID++LFP+  
Sbjct: 891  VHSCEIIHGDIKPDNFILGHR--------FLEQADEDLATGLALIDLGQSIDMKLFPKGT 950

Query: 410  EFEGDCRTSGFRCTEMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLP 469
             F G C TSGF+C EML NKPW YQ+D +G    ++ ML GSYM    KV ++G  V+ P
Sbjct: 951  VFTGKCETSGFQCPEMLSNKPWNYQIDYFGVAATIYCMLFGSYM----KVKNEGG-VWKP 1010

Query: 470  KTSFKRYWNVELWKNLFTKLLNI 488
            +  F+R  ++++W+  F  +LNI
Sbjct: 1011 EGLFRRLPHLDMWEEFFHIMLNI 1011


HSP 2 Score: 54.3 bits (129), Expect = 4.9e-06
Identity = 40/130 (30.77%), Postives = 58/130 (44.62%), Query Frame = 0 

Query: 19  DPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTVKYKNDVRFLKIWFLSIGM 78
           DPL  W   IK   EN          L  L+   +K F     Y ND RF+         
Sbjct: 22  DPLGEWESFIKWVEENFPDNK---EYLMTLLEHLMKEFLHKKNYHNDSRFINYCLKFAEY 81

Query: 79  REDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYEIGLLRNAEPLEWLKK 138
             D    F+ L  Q I   +S +Y+ +A  LE++G L  A+ I++ G+   AEP E L++
Sbjct: 82  NSDRHQFFEFLYNQGIGTKSSYIYMSWAGHLEAQGELQHASAIFQTGIHNEAEPKELLQQ 141

Query: 139 AKMLFIDRMS 149
              LF  R++
Sbjct: 142 QYRLFQARLT 148

[Close]
BLAST of CmaCh11G019180 vs. ExPASy Swiss-Prot
Match: P41695 (Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=BUB1 PE=1 SV=2)

HSP 1 Score: 188.0 bits (476), Expect = 2.8e-46
Identity = 107/331 (32.33%), Postives = 178/331 (53.78%), Query Frame = 0 

Query: 173 NPWSSSVMKDLLSKINLKILKYEGYHSSIKPYPKKMALNSL--------RNSSRNKVIEI 232
           NP S+++    LS+I+  + +Y  +++    Y +++ ++SL        RN ++N +++ 
Sbjct: 643 NPLSNNLRAKFLSEISPPLFQYNTFYN----YNQELKMSSLLKKIHRVSRNENKNPIVDF 702

Query: 233 --GGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVK 292
              G  Y I+G  G GG+A VY +  +S+   + ALK++ P   WE+YI  Q++ R+   
Sbjct: 703 KKTGDLYCIRGELGEGGYATVYLA--ESSQGHLRALKVEKPASVWEYYIMSQVEFRLRKS 762

Query: 293 E-RSSFGCAHEMYLYSDYSILICDYLAHGTLQDAINLY----VVLGKTMEEVLCIYYTIE 352
               S   A  ++L+ D S L+ +Y + GT+ D INL     +     M+E LC++ T+E
Sbjct: 763 TILKSIINASALHLFLDESYLVLNYASQGTVLDLINLQREKAIDGNGIMDEYLCMFITVE 822

Query: 353 MLYMLETLHNIGIIHGDFKPDNLLIRYAR--GKLTEDGFRERSGPWNDQGLCLVDWGRGI 412
           ++ +LE +H +GIIHGD KPDN +IR  +    L     R     W ++G+ L+D+GR  
Sbjct: 823 LMKVLEKIHEVGIIHGDLKPDNCMIRLEKPGEPLGAHYMRNGEDGWENKGIYLIDFGRSF 882

Query: 413 DLQLFPENVEFEGDCRTSGFRCTEMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVS 472
           D+ L P   +F+ + +     C EM   KPW Y+ D YG   V+H+ML G +++  +  +
Sbjct: 883 DMTLLPPGTKFKSNWKADQQDCWEMRAGKPWSYEADYYGLAGVIHSMLFGKFIETIQLQN 942

Query: 473 SDGSFVYLPKTSFKRYWNVELWKNLFTKLLN 487
                    K  FKRYW  E+W  +F  LLN
Sbjct: 943 GRCKL----KNPFKRYWKKEIWGVIFDLLLN 963

[Close]
BLAST of CmaCh11G019180 vs. ExPASy Swiss-Prot
Match: O94751 (Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=bub1 PE=1 SV=1)

HSP 1 Score: 174.9 bits (442), Expect = 2.5e-42
Identity = 129/420 (30.71%), Postives = 202/420 (48.10%), Query Frame = 0 

Query: 155  SMKLNDGGESTKFVETYVNPWSSSVMKDLLSKINLKILKYEGYH---------------- 214
            +M   +   S   + T +NP   S+   L   +   +L+ + YH                
Sbjct: 623  TMPYEEAMASLSNLPTLINPLDQSLRDLLFQVLRPSLLRDKDYHEHETSFALVEHIESFV 682

Query: 215  SSIKPYPKKMALNSLRNSSRNKVIEIGGKKY----------EIKGCAGTGGFAQVY--KS 274
            S IK  PK       R+S+R+    + G ++           +    G G FA VY  KS
Sbjct: 683  SKIK--PKAGGPGRRRSSNRH---SLDGPEFHLFYPPNTNLSVISKLGQGAFAPVYLVKS 742

Query: 275  YLDSNPDDV------------VALKIQTPPFPWEFYIYRQLDQRVT-VKERSSFGCAHEM 334
             +++   DV             ALKI+TPP  +EFY+ RQ   R+  ++E +S    H++
Sbjct: 743  KIETENGDVSQGGAENNESKLFALKIETPPSCFEFYLTRQAMTRLKGLRETNSILPVHQL 802

Query: 335  YLYSDYSILICDYLAHGTLQDAINLY---VVLGKTMEEVLCIYYTIEMLYMLETLHNIGI 394
            +++ D S L+ DY   G++ D +N           M+E+L ++++IE L ++E LH   I
Sbjct: 803  HMFHDTSHLLMDYRPQGSILDLVNSMHNSTFSSSGMDEILVVFFSIEFLRIIEALHTHKI 862

Query: 395  IHGDFKPDNLLIR---YARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFE 454
            IHGD K DN L+R    A  + +     E    W+ +G+ L+D+GRGIDL LF E V+F 
Sbjct: 863  IHGDLKADNALLRLETVADSEWSPIYSPEGLYGWSFKGIYLIDFGRGIDLSLFEEKVKFI 922

Query: 455  GDCRTSGFRCTEMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTS 514
             D  T    C EM E +PW YQ+D +G   +++TML G Y++   +V  +G    +    
Sbjct: 923  ADWDTDLQDCIEMREGRPWTYQIDYHGLAAIIYTMLFGQYIETRIEV-INGQRRQVLTQR 982

Query: 515  FKRYWNVELWKNLFTKLLN--IKLGDYN---KELLQSIRGSFQDYMCSNPQLIRKLKELL 523
             KRYWN +LW  LF  LLN  + + + N    E L  IR   ++++ ++      LK LL
Sbjct: 983  MKRYWNQDLWHRLFDLLLNPTLHVSEENLPMTEELSKIRIEMEEWLVNHSTGGSGLKGLL 1036

[Close]
BLAST of CmaCh11G019180 vs. ExPASy TrEMBL
Match: A0A6J1JF74 (mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita maxima OX=3661 GN=LOC111486358 PE=4 SV=1)

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 532/532 (100.00%), Postives = 532/532 (100.00%), Query Frame = 0 

Query: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60
           MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV
Sbjct: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180
           IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM
Sbjct: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480

Query: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 532

[Close]
BLAST of CmaCh11G019180 vs. ExPASy TrEMBL
Match: A0A6J1EXJ7 (mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita moschata OX=3662 GN=LOC111437097 PE=4 SV=1)

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 524/532 (98.50%), Postives = 528/532 (99.25%), Query Frame = 0 

Query: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60
           MAII PDSQIT+TTTS QDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFG TV
Sbjct: 58  MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 117

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 118 KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 177

Query: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180
           IYE+GLLRNAEPLEWLKKAK+LFIDRMSELVNTHSMKLNDGGESTKFVE+YVNPWSSSVM
Sbjct: 178 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 237

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 238 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 297

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS
Sbjct: 298 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 357

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL
Sbjct: 358 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 417

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 418 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 477

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 478 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 537

Query: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           FTKLLNIKLGDYNKELLQSIR SFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 538 FTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 589

[Close]
BLAST of CmaCh11G019180 vs. ExPASy TrEMBL
Match: A0A6J1D2B6 (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3 OS=Momordica charantia OX=3673 GN=LOC111016707 PE=4 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 1.8e-282
Identity = 466/532 (87.59%), Postives = 491/532 (92.29%), Query Frame = 0 

Query: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60
           MAIIFPDSQ TS T S QDPLLPWLWSIKDALENSTSGN SGP LA L+SDCIK FG  V
Sbjct: 1   MAIIFPDSQNTS-TISAQDPLLPWLWSIKDALENSTSGNASGPDLAKLLSDCIKNFGGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKND+RFLKIWFLSIG+REDFETGFKELLEQEICIGNSLLYIWYA FLESKG+L+DANM
Sbjct: 61  KYKNDIRFLKIWFLSIGLREDFETGFKELLEQEICIGNSLLYIWYAAFLESKGKLHDANM 120

Query: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180
           +YE+GLLRNAEP EWLKKAK LFIDRMSELV+ HS ++NDGGES KF ETYVNPWS S+M
Sbjct: 121 VYELGLLRNAEPFEWLKKAKTLFIDRMSELVSIHSTQVNDGGESNKFAETYVNPWSPSIM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKIN KILKYEGYHSS K Y +KMALNSLRNSSRNK IEIGGK+YEIKGCAGTGGF
Sbjct: 181 KDLLSKINPKILKYEGYHSSSKAYSRKMALNSLRNSSRNKTIEIGGKRYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYK+Y+D NP+DVVALKIQTPPFPWEFY+YRQLDQ VT+KERSSFGCAH MY YSDYS
Sbjct: 241 AQVYKAYVDGNPEDVVALKIQTPPFPWEFYMYRQLDQHVTMKERSSFGCAHAMYHYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLH  GIIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHGFGIIHGDFKPDNL 360

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYAR  LTEDGFRERSGPW DQGLCLVDWGRGIDL LFPENVEF+GDCRTSGFRCTEM
Sbjct: 361 LIRYARDNLTEDGFRERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYG CVVVHTMLHGSYMDV+KK SS+G F+YLPKTSFKRYWN+ELWKNL
Sbjct: 421 LENKPWKYQVDIYGLCVVVHTMLHGSYMDVDKKASSNGGFIYLPKTSFKRYWNIELWKNL 480

Query: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           FTKLLNI  GDYN++LLQSIR SFQDYMCSNP LIRKLKELL KQRASLCTA
Sbjct: 481 FTKLLNITPGDYNQKLLQSIRVSFQDYMCSNPHLIRKLKELLVKQRASLCTA 531

[Close]
BLAST of CmaCh11G019180 vs. ExPASy TrEMBL
Match: A0A6J1D1C8 (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 OS=Momordica charantia OX=3673 GN=LOC111016707 PE=4 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 1.8e-282
Identity = 466/532 (87.59%), Postives = 491/532 (92.29%), Query Frame = 0 

Query: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60
           MAIIFPDSQ TS T S QDPLLPWLWSIKDALENSTSGN SGP LA L+SDCIK FG  V
Sbjct: 143 MAIIFPDSQNTS-TISAQDPLLPWLWSIKDALENSTSGNASGPDLAKLLSDCIKNFGGNV 202

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKND+RFLKIWFLSIG+REDFETGFKELLEQEICIGNSLLYIWYA FLESKG+L+DANM
Sbjct: 203 KYKNDIRFLKIWFLSIGLREDFETGFKELLEQEICIGNSLLYIWYAAFLESKGKLHDANM 262

Query: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180
           +YE+GLLRNAEP EWLKKAK LFIDRMSELV+ HS ++NDGGES KF ETYVNPWS S+M
Sbjct: 263 VYELGLLRNAEPFEWLKKAKTLFIDRMSELVSIHSTQVNDGGESNKFAETYVNPWSPSIM 322

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKIN KILKYEGYHSS K Y +KMALNSLRNSSRNK IEIGGK+YEIKGCAGTGGF
Sbjct: 323 KDLLSKINPKILKYEGYHSSSKAYSRKMALNSLRNSSRNKTIEIGGKRYEIKGCAGTGGF 382

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYK+Y+D NP+DVVALKIQTPPFPWEFY+YRQLDQ VT+KERSSFGCAH MY YSDYS
Sbjct: 383 AQVYKAYVDGNPEDVVALKIQTPPFPWEFYMYRQLDQHVTMKERSSFGCAHAMYHYSDYS 442

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLH  GIIHGDFKPDNL
Sbjct: 443 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHGFGIIHGDFKPDNL 502

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYAR  LTEDGFRERSGPW DQGLCLVDWGRGIDL LFPENVEF+GDCRTSGFRCTEM
Sbjct: 503 LIRYARDNLTEDGFRERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCTEM 562

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYG CVVVHTMLHGSYMDV+KK SS+G F+YLPKTSFKRYWN+ELWKNL
Sbjct: 563 LENKPWKYQVDIYGLCVVVHTMLHGSYMDVDKKASSNGGFIYLPKTSFKRYWNIELWKNL 622

Query: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           FTKLLNI  GDYN++LLQSIR SFQDYMCSNP LIRKLKELL KQRASLCTA
Sbjct: 623 FTKLLNITPGDYNQKLLQSIRVSFQDYMCSNPHLIRKLKELLVKQRASLCTA 673

[Close]
BLAST of CmaCh11G019180 vs. ExPASy TrEMBL
Match: A0A6J1D2I5 (mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2 OS=Momordica charantia OX=3673 GN=LOC111016707 PE=4 SV=1)

HSP 1 Score: 981.1 bits (2535), Expect = 1.8e-282
Identity = 466/532 (87.59%), Postives = 491/532 (92.29%), Query Frame = 0 

Query: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60
           MAIIFPDSQ TS T S QDPLLPWLWSIKDALENSTSGN SGP LA L+SDCIK FG  V
Sbjct: 131 MAIIFPDSQNTS-TISAQDPLLPWLWSIKDALENSTSGNASGPDLAKLLSDCIKNFGGNV 190

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKND+RFLKIWFLSIG+REDFETGFKELLEQEICIGNSLLYIWYA FLESKG+L+DANM
Sbjct: 191 KYKNDIRFLKIWFLSIGLREDFETGFKELLEQEICIGNSLLYIWYAAFLESKGKLHDANM 250

Query: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180
           +YE+GLLRNAEP EWLKKAK LFIDRMSELV+ HS ++NDGGES KF ETYVNPWS S+M
Sbjct: 251 VYELGLLRNAEPFEWLKKAKTLFIDRMSELVSIHSTQVNDGGESNKFAETYVNPWSPSIM 310

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKIN KILKYEGYHSS K Y +KMALNSLRNSSRNK IEIGGK+YEIKGCAGTGGF
Sbjct: 311 KDLLSKINPKILKYEGYHSSSKAYSRKMALNSLRNSSRNKTIEIGGKRYEIKGCAGTGGF 370

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYK+Y+D NP+DVVALKIQTPPFPWEFY+YRQLDQ VT+KERSSFGCAH MY YSDYS
Sbjct: 371 AQVYKAYVDGNPEDVVALKIQTPPFPWEFYMYRQLDQHVTMKERSSFGCAHAMYHYSDYS 430

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLH  GIIHGDFKPDNL
Sbjct: 431 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHGFGIIHGDFKPDNL 490

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYAR  LTEDGFRERSGPW DQGLCLVDWGRGIDL LFPENVEF+GDCRTSGFRCTEM
Sbjct: 491 LIRYARDNLTEDGFRERSGPWQDQGLCLVDWGRGIDLHLFPENVEFKGDCRTSGFRCTEM 550

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYG CVVVHTMLHGSYMDV+KK SS+G F+YLPKTSFKRYWN+ELWKNL
Sbjct: 551 LENKPWKYQVDIYGLCVVVHTMLHGSYMDVDKKASSNGGFIYLPKTSFKRYWNIELWKNL 610

Query: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           FTKLLNI  GDYN++LLQSIR SFQDYMCSNP LIRKLKELL KQRASLCTA
Sbjct: 611 FTKLLNITPGDYNQKLLQSIRVSFQDYMCSNPHLIRKLKELLVKQRASLCTA 661

[Close]
BLAST of CmaCh11G019180 vs. NCBI nr
Match: XP_022989217.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita maxima])

HSP 1 Score: 1101.7 bits (2848), Expect = 0.0e+00
Identity = 532/532 (100.00%), Postives = 532/532 (100.00%), Query Frame = 0 

Query: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60
           MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV
Sbjct: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180
           IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM
Sbjct: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480

Query: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 532

[Close]
BLAST of CmaCh11G019180 vs. NCBI nr
Match: XP_022930700.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita moschata])

HSP 1 Score: 1088.2 bits (2813), Expect = 0.0e+00
Identity = 524/532 (98.50%), Postives = 528/532 (99.25%), Query Frame = 0 

Query: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60
           MAII PDSQIT+TTTS QDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFG TV
Sbjct: 58  MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 117

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 118 KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 177

Query: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180
           IYE+GLLRNAEPLEWLKKAK+LFIDRMSELVNTHSMKLNDGGESTKFVE+YVNPWSSSVM
Sbjct: 178 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 237

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 238 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 297

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS
Sbjct: 298 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 357

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL
Sbjct: 358 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 417

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 418 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 477

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 478 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 537

Query: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           FTKLLNIKLGDYNKELLQSIR SFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 538 FTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 589

[Close]
BLAST of CmaCh11G019180 vs. NCBI nr
Match: KAG6589257.1 (Mitotic checkpoint serine/threonine-protein kinase BUB1, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1085.9 bits (2807), Expect = 0.0e+00
Identity = 523/532 (98.31%), Postives = 527/532 (99.06%), Query Frame = 0 

Query: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60
           MAII PDSQIT+TTTS QDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFG TV
Sbjct: 1   MAIIIPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180
           IYE+GLLRNAEPLEWLKKAK+LFIDRMSELVNTHSMKLNDGGESTKFVE+YVNPWSSSVM
Sbjct: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480

Query: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           FTKLLNIKLGDYN ELLQSIR SFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 481 FTKLLNIKLGDYNMELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532

[Close]
BLAST of CmaCh11G019180 vs. NCBI nr
Match: KAG7022957.1 (Mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1082.4 bits (2798), Expect = 0.0e+00
Identity = 524/534 (98.13%), Postives = 528/534 (98.88%), Query Frame = 0 

Query: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60
           MAII PDSQIT+TTTS QDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFG TV
Sbjct: 1   MAIIIPDSQITTTTTSSQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGTV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180
           IYE+GLLRNAEPLEWLKKAK+LFIDRMSELVNTHSMKLNDGGESTKFVE+YVNPWSSSVM
Sbjct: 121 IYELGLLRNAEPLEWLKKAKVLFIDRMSELVNTHSMKLNDGGESTKFVESYVNPWSSSVM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEI--GGKKYEIKGCAGTG 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEI  GGKKYEIKGCAGTG
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGNGGKKYEIKGCAGTG 240

Query: 241 GFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSD 300
           GFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSD
Sbjct: 241 GFAQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSD 300

Query: 301 YSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPD 360
           YSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPD
Sbjct: 301 YSILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPD 360

Query: 361 NLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCT 420
           NLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCT
Sbjct: 361 NLLIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCT 420

Query: 421 EMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWK 480
           EMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWK
Sbjct: 421 EMLENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWK 480

Query: 481 NLFTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           NLFTKLLNIKLGDYNKELLQSIR SFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 481 NLFTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 534

[Close]
BLAST of CmaCh11G019180 vs. NCBI nr
Match: XP_023531077.1 (mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1082.0 bits (2797), Expect = 0.0e+00
Identity = 522/532 (98.12%), Postives = 524/532 (98.50%), Query Frame = 0 

Query: 1   MAIIFPDSQITSTTTSVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTV 60
           MAIIFPDSQIT+TTTS QDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFG  V
Sbjct: 1   MAIIFPDSQITTTTTSAQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGGNV 60

Query: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120
           KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM
Sbjct: 61  KYKNDVRFLKIWFLSIGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANM 120

Query: 121 IYEIGLLRNAEPLEWLKKAKMLFIDRMSELVNTHSMKLNDGGESTKFVETYVNPWSSSVM 180
           IYE+GLLRNAEPLEWLKKAKMLFIDRMSELVNT SMKLNDGGESTK VETYVNPWSSSVM
Sbjct: 121 IYELGLLRNAEPLEWLKKAKMLFIDRMSELVNTRSMKLNDGGESTKIVETYVNPWSSSVM 180

Query: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240
           KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF
Sbjct: 181 KDLLSKINLKILKYEGYHSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGF 240

Query: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYS 300
           AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLD RVTVKERSSFGCAHEMYLYSDYS
Sbjct: 241 AQVYKSYLDSNPDDVVALKIQTPPFPWEFYIYRQLDHRVTVKERSSFGCAHEMYLYSDYS 300

Query: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNL 360
           ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNI IIHGDFKPDNL
Sbjct: 301 ILICDYLAHGTLQDAINLYVVLGKTMEEVLCIYYTIEMLYMLETLHNISIIHGDFKPDNL 360

Query: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420
           LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM
Sbjct: 361 LIRYARGKLTEDGFRERSGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEM 420

Query: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480
           LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL
Sbjct: 421 LENKPWKYQVDIYGFCVVVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNL 480

Query: 481 FTKLLNIKLGDYNKELLQSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           FTKLLNIKLGDYNKELLQSIR SFQDYMCSNPQLIRKLKELLAKQRASLCTA
Sbjct: 481 FTKLLNIKLGDYNKELLQSIRESFQDYMCSNPQLIRKLKELLAKQRASLCTA 532

[Close]
BLAST of CmaCh11G019180 vs. TAIR 10
Match: AT2G20635.1 (ATP binding;protein kinases;protein serine/threonine kinases )

HSP 1 Score: 670.6 bits (1729), Expect = 1.0e-192
Identity = 308/515 (59.81%), Postives = 399/515 (77.48%), Query Frame = 0 

Query: 19  DPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTVKYKNDVRFLKIWFLSIGM 78
           DPL PWL  IK+++E+  +G  SG  L  L+ DCI T+    +Y+ND+RFLKIWFL +  
Sbjct: 11  DPLFPWLMEIKNSMEDLYAGKNSGYDLDKLLFDCISTYKKDSRYRNDLRFLKIWFLYLEG 70

Query: 79  REDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYEIGLLRNAEPLEWLKK 138
           REDFE  ++E+ E EIC G+SLLY WYA+FLE KG    AN +Y+ GL R AEP + LK+
Sbjct: 71  REDFERVYREIEETEICKGHSLLYEWYAIFLEVKGLWRRANSVYQTGLSRKAEPFDRLKE 130

Query: 139 AKMLFIDRMSELVNTHSM-KLNDGGESTKFVETYVNPWSSSVMKDLLSKINLKILKYEGY 198
           A  LF+ R+S+     S+ K+ D  ++T     +VNPW +S +  L+ KI  +++KY+GY
Sbjct: 131 AHSLFLQRISKRTKASSLEKVGDDAQATDLETGFVNPWETSTVNGLIHKIKPQLVKYDGY 190

Query: 199 HSSIKPYPKKMALNSLRNSSRNKVIEIGGKKYEIKGCAGTGGFAQVYKSYLDSNPDDVVA 258
           H S K +P K  L+SL+N SRNK+IEIGG+KY++KGCAG GGFAQV+K+++DSNPD+VVA
Sbjct: 191 HVSNKVFPGKANLSSLQNYSRNKIIEIGGRKYQMKGCAGQGGFAQVFKAFIDSNPDEVVA 250

Query: 259 LKIQTPPFPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYSILICDYLAHGTLQDAIN 318
           LK+Q PPFPWEF++YRQLD R+   +RSSFG A  +++YSDYSIL+CDYL+HGTLQD IN
Sbjct: 251 LKVQKPPFPWEFHMYRQLDCRIPDSQRSSFGLAQRVHVYSDYSILVCDYLSHGTLQDVIN 310

Query: 319 LYVVLGKTMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNLLIRYARGKLTEDGFRER 378
            YVV+GK+MEEVLC+YYTIEML MLETLH++GIIHGDFKPDNLLIRY    LTE GF E+
Sbjct: 311 SYVVVGKSMEEVLCMYYTIEMLNMLETLHSVGIIHGDFKPDNLLIRYPPENLTETGFHEK 370

Query: 379 SGPWNDQGLCLVDWGRGIDLQLFPENVEFEGDCRTSGFRCTEMLENKPWKYQVDIYGFCV 438
           +G W+ +GLCLVDWGRGIDL LFP   EF GDCRTSGFRC EM E+KPWK+QVD YG CV
Sbjct: 371 TGSWSKKGLCLVDWGRGIDLSLFPRTTEFTGDCRTSGFRCVEMKEDKPWKFQVDTYGLCV 430

Query: 439 VVHTMLHGSYMDVEKKVSSDGSFVYLPKTSFKRYWNVELWKNLFTKLLNIKLGDYNKELL 498
           +VH MLH  YM++EKK S DG ++ +P+TSFKRYW V+LWK LFTKLLN +  + + E L
Sbjct: 431 IVHMMLHNVYMEIEKKQSLDGGYINMPRTSFKRYWKVDLWKELFTKLLNRETCEDDTETL 490

Query: 499 QSIRGSFQDYMCSNPQLIRKLKELLAKQRASLCTA 533
           +++R S ++Y+CS+P+L++KL ELLAKQR SLC++
Sbjct: 491 RNLRKSMEEYICSDPKLMKKLNELLAKQRISLCSS 525

[Close]
BLAST of CmaCh11G019180 vs. TAIR 10
Match: AT2G33560.1 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 81.6 bits (200), Expect = 2.0e-15
Identity = 53/187 (28.34%), Postives = 91/187 (48.66%), Query Frame = 0 

Query: 19  DPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTVKYKNDVRFLKIWFLSIGM 78
           DPL PW+  IK   E    G     GL  +   C++ F  + +YK+D+R+LK+W      
Sbjct: 80  DPLSPWIECIKWVQEAFPPGGECS-GLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEH 139

Query: 79  REDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYEIGLLRNAEPLEWLKK 138
             D E  +K L   EI   +++ YI YA+ +E K ++  AN I+ +G+ R+A+P+E L  
Sbjct: 140 CADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLND 199

Query: 139 AKMLFIDRMSELVNTHSMKLNDGGE-STKFVETYVNPWSSSVMKDLLSKINLKILKYEGY 198
           A   F+ R     NT   +  +  +  ++   T ++   ++  +  L   N +  K +  
Sbjct: 200 AYKKFMVRTMRRSNTADEEPKENNDLPSRSFGTLLSRGDNNARRQALGSSNPQAKKLKPN 259

Query: 199 HSSIKPY 205
            SS  P+
Sbjct: 260 QSSKTPF 265

[Close]
BLAST of CmaCh11G019180 vs. TAIR 10
Match: AT2G33560.2 (BUB1-related (BUB1: budding uninhibited by benzymidazol 1) )

HSP 1 Score: 81.3 bits (199), Expect = 2.7e-15
Identity = 57/189 (30.16%), Postives = 91/189 (48.15%), Query Frame = 0 

Query: 19  DPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTVKYKNDVRFLKIWFLSIGM 78
           DPL PW+  IK   E    G     GL  +   C++ F  + +YK+D+R+LK+W      
Sbjct: 80  DPLSPWIECIKWVQEAFPPGGECS-GLLVIYEQCVRKFWHSERYKDDLRYLKVWLEYAEH 139

Query: 79  REDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYEIGLLRNAEPLEWLKK 138
             D E  +K L   EI   +++ YI YA+ +E K ++  AN I+ +G+ R+A+P+E L  
Sbjct: 140 CADAEVIYKFLEVNEIGKTHAVYYIAYALHIEFKNKVKTANEIFNLGISRDAKPVEKLND 199

Query: 139 AKMLFIDRMSELVNT---HSMKLNDGGESTKFVETYVNPWSSSVMKDLLSKINLKILKYE 198
           A   F+ R     NT      K N+   S  F  T ++   ++  +  L   N +  K +
Sbjct: 200 AYKKFMVRTMRRSNTADEQEPKENNDLPSRSF-GTLLSRGDNNARRQALGSSNPQAKKLK 259

Query: 199 GYHSSIKPY 205
              SS  P+
Sbjct: 260 PNQSSKTPF 266

[Close]
BLAST of CmaCh11G019180 vs. TAIR 10
Match: AT5G05510.1 (Mad3/BUB1 homology region 1 )

HSP 1 Score: 75.1 bits (183), Expect = 1.9e-13
Identity = 39/132 (29.55%), Postives = 68/132 (51.52%), Query Frame = 0 

Query: 16  SVQDPLLPWLWSIKDALENSTSGNVSGPGLADLVSDCIKTFGSTVKYKNDVRFLKIWFLS 75
           S +D LLPW+  +K  ++ S    +    L   +  C ++F S  +YKND R++++W   
Sbjct: 23  SGKDHLLPWIRGVK-KMKESLPSQILNEKLPRFLQKCAESFESDKRYKNDSRYIRVWLQL 82

Query: 76  IGMREDFETGFKELLEQEICIGNSLLYIWYAVFLESKGRLNDANMIYEIGLLRNAEPLEW 135
           +   +D     + +  + I    SL Y  YA+  E   R  DA  +Y +G+   AEP++ 
Sbjct: 83  MDFVDDPRALLRTMEAKSIGTKRSLFYQAYALHYEKMKRFEDAEKMYRLGVQNLAEPMDE 142

Query: 136 LKKAKMLFIDRM 148
           L+K+ + F+ RM
Sbjct: 143 LQKSYLQFVTRM 153

[Close]
BLAST of CmaCh11G019180 vs. TAIR 10
Match: AT3G03940.1 (Protein kinase family protein )

HSP 1 Score: 52.8 bits (125), Expect = 1.0e-06
Identity = 44/158 (27.85%), Postives = 66/158 (41.77%), Query Frame = 0 

Query: 222 IEIGGKK-YEIKGCAGTGGFAQVYKSYLDSNPDD-------VVALKIQ---------TPP 281
           +++G    Y+ +   G GGF QVY     S   D        VALK++          PP
Sbjct: 132 VQVGNSPVYKTERKLGKGGFGQVYVGRRVSGGSDRIGADAIEVALKLEHRNSKGCNFGPP 191

Query: 282 FPWEFYIYRQLDQRVTVKERSSFGCAHEMYLYSDYSILICDYLAHGTLQDAINLYVVLGK 341
           + W+ Y         T+         H      D+ IL+ D L   +L D  N    L +
Sbjct: 192 YEWQVY--------NTLNSCYGIPAVHHKGRQGDFYILVMDMLG-PSLWDVWN---SLAQ 251

Query: 342 TMEEVLCIYYTIEMLYMLETLHNIGIIHGDFKPDNLLI 363
           +M   +     +E + +LE LH  G +HGD KP+N L+
Sbjct: 252 SMSPNMVACIAVEAISILEKLHMKGFVHGDVKPENFLL 277

The following BLAST results are available for this feature:
BLAST of CmaCh11G019180 vs. ExPASy Swiss-Prot
Match NameE-valueIdentityDescription
F4IVI01.4e-19159.81Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Arabidopsis thaliana ... [more]
O436833.3e-4735.20Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Homo sapiens OX=9606 ... [more]
O089014.4e-4733.44Mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Mus musculus OX=10090... [more]
P416952.8e-4632.33Checkpoint serine/threonine-protein kinase BUB1 OS=Saccharomyces cerevisiae (str... [more]
O947512.5e-4230.71Checkpoint serine/threonine-protein kinase bub1 OS=Schizosaccharomyces pombe (st... [more]
BLAST of CmaCh11G019180 vs. ExPASy TrEMBL
Match NameE-valueIdentityDescription
A0A6J1JF740.0e+00100.00mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita maxima OX=3... [more]
A0A6J1EXJ70.0e+0098.50mitotic checkpoint serine/threonine-protein kinase BUB1 OS=Cucurbita moschata OX... [more]
A0A6J1D2B61.8e-28287.59mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X3 OS=Momordica ... [more]
A0A6J1D1C81.8e-28287.59mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X1 OS=Momordica ... [more]
A0A6J1D2I51.8e-28287.59mitotic checkpoint serine/threonine-protein kinase BUB1 isoform X2 OS=Momordica ... [more]
BLAST of CmaCh11G019180 vs. NCBI nr
Match NameE-valueIdentityDescription
XP_022989217.10.0e+00100.00mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita maxima][more]
XP_022930700.10.0e+0098.50mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita moschata][more]
KAG6589257.10.0e+0098.31Mitotic checkpoint serine/threonine-protein kinase BUB1, partial [Cucurbita argy... [more]
KAG7022957.10.0e+0098.13Mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita argyrosperma ... [more]
XP_023531077.10.0e+0098.12mitotic checkpoint serine/threonine-protein kinase BUB1 [Cucurbita pepo subsp. p... [more]
BLAST of CmaCh11G019180 vs. TAIR 10
Match NameE-valueIdentityDescription
AT2G20635.11.0e-19259.81ATP binding;protein kinases;protein serine/threonine kinases [more]
AT2G33560.12.0e-1528.34BUB1-related (BUB1: budding uninhibited by benzymidazol 1) [more]
AT2G33560.22.7e-1530.16BUB1-related (BUB1: budding uninhibited by benzymidazol 1) [more]
AT5G05510.11.9e-1329.55Mad3/BUB1 homology region 1 [more]
AT3G03940.11.0e-0627.85Protein kinase family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000719Protein kinase domainSMARTSM00220serkin_6coord: 229..522
e-value: 5.4E-9
score: -6.3
IPR000719Protein kinase domainPFAMPF00069Pkinasecoord: 230..447
e-value: 1.2E-15
score: 57.6
IPR000719Protein kinase domainPROSITEPS50011PROTEIN_KINASE_DOMcoord: 229..532
score: 17.078833
IPR013212Mad3/Bub1 homology region 1SMARTSM00777mad3_bub1_icoord: 8..126
e-value: 1.8E-9
score: 36.9
IPR013212Mad3/Bub1 homology region 1PFAMPF08311Mad3_BUB1_Icoord: 10..126
e-value: 9.8E-22
score: 77.2
IPR013212Mad3/Bub1 homology region 1PROSITEPS51489BUB1_Ncoord: 6..164
score: 31.51321
NoneNo IPR availableGENE3D1.10.510.10Transferase(Phosphotransferase) domain 1coord: 227..529
e-value: 1.1E-97
score: 328.2
NoneNo IPR availableGENE3D1.25.40.430coord: 7..160
e-value: 4.1E-33
score: 116.3
NoneNo IPR availablePANTHERPTHR14030:SF4BUB1 KINASE, ISOFORM A-RELATEDcoord: 17..148
coord: 169..531
IPR015661Mitotic spindle checkpoint protein Bub1/Mad3PANTHERPTHR14030MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1coord: 169..531
IPR015661Mitotic spindle checkpoint protein Bub1/Mad3PANTHERPTHR14030MITOTIC CHECKPOINT SERINE/THREONINE-PROTEIN KINASE BUB1coord: 17..148
IPR008271Serine/threonine-protein kinase, active sitePROSITEPS00108PROTEIN_KINASE_STcoord: 350..362
IPR011009Protein kinase-like domain superfamilySUPERFAMILY56112Protein kinase-like (PK-like)coord: 226..455

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G019180.1CmaCh11G019180.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0032147 activation of protein kinase activity
biological_process GO:0051754 meiotic sister chromatid cohesion, centromeric
biological_process GO:0007094 mitotic spindle assembly checkpoint signaling
biological_process GO:0060236 regulation of mitotic spindle organization
biological_process GO:0006468 protein phosphorylation
cellular_component GO:0005737 cytoplasm
cellular_component GO:0000776 kinetochore
cellular_component GO:0005874 microtubule
cellular_component GO:0005634 nucleus
cellular_component GO:0005819 spindle
molecular_function GO:0005524 ATP binding
molecular_function GO:0004672 protein kinase activity