CmaCh11G018620 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh11G018620
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Lipoyl synthase, mitochondrial
Location	Cma_Chr11: 12390664 .. 12392709 (+)
RNA-Seq Expression	CmaCh11G018620
Synteny	CmaCh11G018620

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGAACACCCGTCGCCATAGCCTTGCTCGAATCTTCGAATCCATTGGCAGGTCCTTATCTTATTCATCTTCCTCGACCTCCGCCTCTGCTACCTCCTCCCAAGTCCCTCGGACTTTGGCCGGACTCCGGGAACGACTTGCCGCCGAATCCCCCACGCTTTCTGACTTTGTTAGCCTGAAATCTTCTGATTCGTATTCCGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCCGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAACTCCGCGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTGGGGGAATGCTGGTCTGGTGGAGAAACGGGCACGGCCACTGCTACGATTATGATTCTTGGAGACACTTGTACCCGGGGCTGCAGGCTAGTCATAATTCAATTTACTCATTCATTTCGTTGTGTTTACTTAATTTCCAGTGTTTTTGATGATTGAAGAATGATATCTTCTCGTATGTGCTGTGCATAGTGTATATAATTTACTGAGGAATGTAAATCTCTAATTGTTTTTGTCATAGAAATGATCTTGTCGATTACTATGATATCATCTTATCACTTAGAATTCTATTCATTCCTTGCTATTTTCGGATTATTTGCACTCCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCAGACCCCAATGAGCCGCAAAATGTTGCTGAGGCAATCGCATCATGGGGGTTGGATTATGTGGTTATCACTAGTGTGGATCGCGACGATTTACCTGATCAGGGGAGTGGTCATTTTGCAGAGACCGTGCTGAAACTTAAGGTGTTGAAGCCAAATATGTTGATCGAAGCATTGGGTATGAATTCCCTAGCAGTTTCCTTAGTGTCCCGTTCCTTGTTACATTTAAGGATTCTTGTGTGACACTTGATTTTTGGGAGAGTTATTCTATCTTGTTTTCATCCAATCCAGCTTAGAAATTTAGTGAAAGCTACATACTTACCCGTGTGTAAGTGCAGTCACAAAAATCAGCATCATAGGCTCGTTTGATTCTGATTACTTTTGTCCAGTTTGATTTAATCTGTTCTTTGATGTTCCTTGAACTAATTGATTTTGCAGTCCCTGATTTTCGAGGGGACCCTGGCAGTGTAGAGAAAGTTGCAAAGTCGGGCCTTGACGTCTTTGCTCATAATATAGAGACAGTTGAAGAGCTTCAGGGAGCAGTTCGGGACCATCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCAAAAGAGTTTGCTCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCTGCTGGAGTTGATGTGATGACATTTGGTCAGTACATGAGACCTTCAAAGCGCCACATGCCTGTATCAGAATACATTACACCAGAGGCTTTCGAGAAGTATCACAATCTTGGCATGAAAATGGTACTCTCTCTTTTTTACTTGAACATTTTGTTTGCGTTTATTGTCTTCTTTGTTGTTTAATTAAGTCTTTAAAGGTTAAAGACTACTTTTTTTTTTTCATTTCTAGCATGGTATCATATTCCCATCTTTGTTATTCTTATAGCTCGCGAATAGTCTTGATTTGCATTTCCTATACCATCCCATCTTCCATTTCAACCATTGTAGTTTTATGTGTATTATATTCAAATTGTTCGGGGAGACGATGCTTTACTTTTAGGTATATTAATTCATGTTGTGATGCATAATCCTCATCTCCTCCATCAAGTATCACATGATCAATAGTTTTTAATGTATGGAGTTACTTGCCATGCCTTGAGGATACAACAATGATTTGAAAATCCATTTTCTGTTTGGTTGTGACAGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACATCCTACACAACCTTGA

mRNA sequence

ATGAACACCCGTCGCCATAGCCTTGCTCGAATCTTCGAATCCATTGGCAGGTCCTTATCTTATTCATCTTCCTCGACCTCCGCCTCTGCTACCTCCTCCCAAGTCCCTCGGACTTTGGCCGGACTCCGGGAACGACTTGCCGCCGAATCCCCCACGCTTTCTGACTTTGTTAGCCTGAAATCTTCTGATTCGTATTCCGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCCGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAACTCCGCGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTGGGGGAATGCTGGTCTGGTGGAGAAACGGGCACGGCCACTGCTACGATTATGATTCTTGGAGACACTTGTACCCGGGGCTGCAGGCTAAATTCTATTCATTCCTTGCTATTTTCGGATTATTTGCACTCCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCAGACCCCAATGAGCCGCAAAATGTTGCTGAGGCAATCGCATCATGGGGGTTGGATTATGTGGTTATCACTAGTGTGGATCGCGACGATTTACCTGATCAGGGGAGTGGTCATTTTGCAGAGACCGTGCTGAAACTTAAGGTGTTGAAGCCAAATATGTTGATCGAAGCATTGGTCCCTGATTTTCGAGGGGACCCTGGCAGTGTAGAGAAAGTTGCAAAGTCGGGCCTTGACGTCTTTGCTCATAATATAGAGACAGTTGAAGAGCTTCAGGGAGCAGTTCGGGACCATCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCAAAAGAGTTTGCTCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCTGCTGGAGTTGATGTGATGACATTTGGTCAGTACATGAGACCTTCAAAGCGCCACATGCCTGTATCAGAATACATTACACCAGAGGCTTTCGAGAAGTATCACAATCTTGGCATGAAAATGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACATCCTACACAACCTTGA

Coding sequence (CDS)

ATGAACACCCGTCGCCATAGCCTTGCTCGAATCTTCGAATCCATTGGCAGGTCCTTATCTTATTCATCTTCCTCGACCTCCGCCTCTGCTACCTCCTCCCAAGTCCCTCGGACTTTGGCCGGACTCCGGGAACGACTTGCCGCCGAATCCCCCACGCTTTCTGACTTTGTTAGCCTGAAATCTTCTGATTCGTATTCCGTGGAGGTTGGTACGAAGAAGAAGCCCCTTCCTAAACCTAAATGGATGAAGGAATCGGTGCCCGGTGGCGAGAAGTATGTTCAAATCAAGAAGAAACTCCGCGAATTGAAGCTTCATACGGTTTGTGAGGAGGCTAAATGCCCTAATTTGGGGGAATGCTGGTCTGGTGGAGAAACGGGCACGGCCACTGCTACGATTATGATTCTTGGAGACACTTGTACCCGGGGCTGCAGGCTAAATTCTATTCATTCCTTGCTATTTTCGGATTATTTGCACTCCAGGTTCTGTAACGTGAAGACATCACGAACTCCACCTCCACCAGACCCCAATGAGCCGCAAAATGTTGCTGAGGCAATCGCATCATGGGGGTTGGATTATGTGGTTATCACTAGTGTGGATCGCGACGATTTACCTGATCAGGGGAGTGGTCATTTTGCAGAGACCGTGCTGAAACTTAAGGTGTTGAAGCCAAATATGTTGATCGAAGCATTGGTCCCTGATTTTCGAGGGGACCCTGGCAGTGTAGAGAAAGTTGCAAAGTCGGGCCTTGACGTCTTTGCTCATAATATAGAGACAGTTGAAGAGCTTCAGGGAGCAGTTCGGGACCATCGTGCTAATTTTAAGCAATCATTAGATGTTCTAGTTATGGCAAAAGAGTTTGCTCCTTCTGGAACACTTACGAAGACTTCCATTATGTTAGGCTGTGGGGAAACACCAGATCAAGTCGTGCAAACAATGGAGAAGGTGAGAGCTGCTGGAGTTGATGTGATGACATTTGGTCAGTACATGAGACCTTCAAAGCGCCACATGCCTGTATCAGAATACATTACACCAGAGGCTTTCGAGAAGTATCACAATCTTGGCATGAAAATGGGTTTTCGGTATGTGGCATCTGGCCCAATGGTTAGGTCCTCATACAAGGCAGGAGAATTCTATATCAAGTCAATGATTGAATCAGATCGGGCAGCTTCTAATTCACATCCTACACAACCTTGA

Protein sequence

MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLKSSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQP

Homology

[Close]

BLAST of CmaCh11G018620 vs. ExPASy Swiss-Prot
Match: Q3LSN5 (Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1)

HSP 1 Score: 621.7 bits (1602), Expect = 5.7e-177
Identity = 314/391 (80.31%), Postives = 344/391 (87.98%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           M +R  ++A       + LS SS++T+ ++  S++ + LA LR RLA ESP+LSDF+SLK
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDP+EP N
Sbjct: 122 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPDEPTN 181

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHF ETV KLK LKP+MLIEALVPDFRG+   
Sbjct: 182 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFTETVQKLKALKPSMLIEALVPDFRGNAEC 241

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKV+KSGLDVFAHNIETVEELQ AVRDHRANFKQSLDVL+MAKE+AP+GTLTKTSIMLG
Sbjct: 242 VEKVSKSGLDVFAHNIETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLG 301

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQ+V+TMEKVRAAGVDVMTFGQ+MRPSKRHMPVSEYITPEAFEKY  LGM+MGFR
Sbjct: 302 CGETPDQIVKTMEKVRAAGVDVMTFGQHMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFR 361

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASN 392
           YVASGPMVRSSYKAGEFYIKSMI+SDRAAS+
Sbjct: 362 YVASGPMVRSSYKAGEFYIKSMIDSDRAASS 376

[Close]

BLAST of CmaCh11G018620 vs. ExPASy Swiss-Prot
Match: Q3LSN4 (Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1)

HSP 1 Score: 621.3 bits (1601), Expect = 7.5e-177
Identity = 314/391 (80.31%), Postives = 343/391 (87.72%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           M +R  ++A       + LS SS++T+ ++  S++ + LA LR RLA ESP+LSDF+SLK
Sbjct: 2   MYSRFRTVAGNLNCAAKRLSSSSTTTTTTSAPSELQQNLAALRARLAMESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S ++YSVEVGTKKKPLPKPKWMKES+PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 62  SDNAYSVEVGTKKKPLPKPKWMKESIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDP+EP N
Sbjct: 122 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPDEPTN 181

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHF ETV KLK LKP+ LIEALVPDFRG+   
Sbjct: 182 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFTETVQKLKALKPSTLIEALVPDFRGNAEC 241

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKV+KSGLDVFAHNIETVEELQ AVRDHRANFKQSLDVL+MAKE+AP+GTLTKTSIMLG
Sbjct: 242 VEKVSKSGLDVFAHNIETVEELQSAVRDHRANFKQSLDVLMMAKEYAPAGTLTKTSIMLG 301

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQ+V+TMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY  LGM+MGFR
Sbjct: 302 CGETPDQIVKTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFR 361

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASN 392
           YVASGPMVRSSYKAGEFYIKSMI+SDRAAS+
Sbjct: 362 YVASGPMVRSSYKAGEFYIKSMIDSDRAASS 376

[Close]

BLAST of CmaCh11G018620 vs. ExPASy Swiss-Prot
Match: B9H5L9 (Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 619.4 bits (1596), Expect = 2.8e-176
Identity = 314/374 (83.96%), Postives = 333/374 (89.04%), Query Frame = 0


Query: 23  SSSTSASATSS--QVPRTLAGLRERLAAESPTLSDFVSLKSSDSYSVEVGTKKKPLPKPK 82
           SSST  S+T    Q  +TLAGLR RLA ESPTLSDF+ L+S+++YSVEVGTKKKPLPKPK
Sbjct: 26  SSSTVESSTKQPPQFSQTLAGLRARLAVESPTLSDFIHLQSNNTYSVEVGTKKKPLPKPK 85

Query: 83  WMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCT 142
           WM+E++PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCT
Sbjct: 86  WMREAIPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCT 145

Query: 143 RGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVITSVDR 202
           RGC                RFCNVKTSRTPPPPDPNEP NVAEAIASWGLDYVVITSVDR
Sbjct: 146 RGC----------------RFCNVKTSRTPPPPDPNEPTNVAEAIASWGLDYVVITSVDR 205

Query: 203 DDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNIETVE 262
           DDL DQGSGHFAETV KLK LKPNMLIEALVPDFRGD G VEKVAKSGLDVFAHNIETVE
Sbjct: 206 DDLADQGSGHFAETVHKLKTLKPNMLIEALVPDFRGDRGCVEKVAKSGLDVFAHNIETVE 265

Query: 263 ELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGV 322
           ELQ +VRDHRANFKQSLDVL+MAKE+AP GTLTKTSIMLGCGE P+QVV+TMEKVRAAGV
Sbjct: 266 ELQSSVRDHRANFKQSLDVLMMAKEYAPPGTLTKTSIMLGCGEAPEQVVKTMEKVRAAGV 325

Query: 323 DVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGEFYIK 382
           DVMTFGQYMRPSKRHMPVSEYITP+AFEKY  LGM+MGFRYVASGPMVRSSYKAGEFYIK
Sbjct: 326 DVMTFGQYMRPSKRHMPVSEYITPDAFEKYKTLGMEMGFRYVASGPMVRSSYKAGEFYIK 383

Query: 383 SMIESDRAASNSHP 395
           SMIESDR+ S+  P
Sbjct: 386 SMIESDRSVSSQLP 383

[Close]

BLAST of CmaCh11G018620 vs. ExPASy Swiss-Prot
Match: B7FM45 (Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1)

HSP 1 Score: 609.8 bits (1571), Expect = 2.3e-173
Identity = 305/391 (78.01%), Postives = 342/391 (87.47%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           M +R  ++A+  +S  +  S+++++T+ + +SS+ P+ L  LR RLA ESP+LSDF+SLK
Sbjct: 2   MYSRFRTVAKNLKSTTKPFSFTTATTTTTVSSSEFPQNLTELRARLARESPSLSDFISLK 61

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           S+++YSVEVGTKK PLPKPKWMKES+PGG KYVQIKKKLRELKLHTVCEEAKCPN+GECW
Sbjct: 62  SNNAYSVEVGTKKNPLPKPKWMKESIPGGWKYVQIKKKLRELKLHTVCEEAKCPNMGECW 121

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDP+EP N
Sbjct: 122 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPDEPTN 181

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSV RDDLPDQGS HF ETV KLK+LKP++LIEALVPDFRG+   
Sbjct: 182 VAEAIASWGLDYVVITSVGRDDLPDQGSSHFTETVQKLKILKPSILIEALVPDFRGNAEC 241

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKV+KSGLDVFAHNIETVEELQ AVRDHRANF QSLDVL MAK++AP+GTLTKTSIMLG
Sbjct: 242 VEKVSKSGLDVFAHNIETVEELQSAVRDHRANFNQSLDVLRMAKDYAPAGTLTKTSIMLG 301

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQ+V+TMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY  LGM+MGFR
Sbjct: 302 CGETPDQIVKTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQTLGMEMGFR 361

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASN 392
           YVASGPMVRSSYKAGEFYIKSMI+SDRA S+
Sbjct: 362 YVASGPMVRSSYKAGEFYIKSMIDSDRAVSS 376

[Close]

BLAST of CmaCh11G018620 vs. ExPASy Swiss-Prot
Match: A5CB81 (Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 607.8 bits (1566), Expect = 8.6e-173
Identity = 303/364 (83.24%), Postives = 324/364 (89.01%), Query Frame = 0


Query: 27  SASATSSQVPRTLAGLRERLAAESPTLSDFVSLKSSDSYSVEVGTKKKPLPKPKWMKESV 86
           S+  T+ + P+TLA LR RLA ESP+LSDFV L++SD YSVEVGTKKKPL KPKWMKES+
Sbjct: 34  SSPPTNPEPPQTLASLRHRLAVESPSLSDFVRLQTSDDYSVEVGTKKKPLSKPKWMKESI 93

Query: 87  PGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCRLN 146
           PGG KY QIKKKLR+L LHTVCEEA+CPN+GECWSGGETGTATATIMILGDTCTRGC   
Sbjct: 94  PGGAKYTQIKKKLRQLNLHTVCEEARCPNMGECWSGGETGTATATIMILGDTCTRGC--- 153

Query: 147 SIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVITSVDRDDLPDQ 206
                        RFCNVKTSRTPPPPDP+EP  VAEAIASWGLDYVVITSVDRDDLPDQ
Sbjct: 154 -------------RFCNVKTSRTPPPPDPDEPSKVAEAIASWGLDYVVITSVDRDDLPDQ 213

Query: 207 GSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNIETVEELQGAV 266
           GSGHFAETV KLK+LKPNMLIEALVPDFRGDPG VEKV+KSGLDVFAHNIETVEELQ AV
Sbjct: 214 GSGHFAETVQKLKILKPNMLIEALVPDFRGDPGCVEKVSKSGLDVFAHNIETVEELQSAV 273

Query: 267 RDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMTFG 326
           RDHRANFKQSL+VL +AKE+A +GTLTKTSIMLGCGETPDQVV+TMEKVRAAGVDVMTFG
Sbjct: 274 RDHRANFKQSLEVLKLAKEYADAGTLTKTSIMLGCGETPDQVVRTMEKVRAAGVDVMTFG 333

Query: 327 QYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIESD 386
           QYMRPSKRHMPVSEYITPEAFEKY  LGM MGFRYVASGPMVRSSYKAGEFYIKSMI++D
Sbjct: 334 QYMRPSKRHMPVSEYITPEAFEKYRILGMDMGFRYVASGPMVRSSYKAGEFYIKSMIDAD 381

Query: 387 RAAS 391
           RA S
Sbjct: 394 RAMS 381

[Close]

BLAST of CmaCh11G018620 vs. ExPASy TrEMBL
Match: A0A6J1JNE9 (Lipoyl synthase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111486046 PE=3 SV=1)

HSP 1 Score: 746.5 bits (1926), Expect = 5.7e-212
Identity = 381/397 (95.97%), Postives = 381/397 (95.97%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK
Sbjct: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDPNEPQN
Sbjct: 121 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPNEPQN 180

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS
Sbjct: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG
Sbjct: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR
Sbjct: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQP 398
           YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQP
Sbjct: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQP 381

[Close]

BLAST of CmaCh11G018620 vs. ExPASy TrEMBL
Match: A0A6J1ERM2 (Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437162 PE=3 SV=1)

HSP 1 Score: 736.1 bits (1899), Expect = 7.7e-209
Identity = 375/396 (94.70%), Postives = 377/396 (95.20%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           MN RRHSLARIFES+GRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFV LK
Sbjct: 1   MNARRHSLARIFESVGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDPNEPQN
Sbjct: 121 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPNEPQN 180

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGDPGS
Sbjct: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGS 240

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG
Sbjct: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR
Sbjct: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQ 397
           YVASGPMVRSSYKAGEFYIKSMIESDRAAS+SHPTQ
Sbjct: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASSSHPTQ 380

[Close]

BLAST of CmaCh11G018620 vs. ExPASy TrEMBL
Match: A0A5A7SKJ1 (Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE=3 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 8.0e-198
Identity = 359/396 (90.66%), Postives = 365/396 (92.17%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           MN R  SLARI +S+ RS S SSSSTSASATSSQ PRTLAGLRERLAAESP+LSDFV L+
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDPNEP N
Sbjct: 121 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPNEPTN 180

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGD G 
Sbjct: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGC 240

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKVA+SGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLG
Sbjct: 241 VEKVARSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLG 300

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY NLGMKMGFR
Sbjct: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFR 360

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQ 397
           YVASGPMVRSSYKAGEFYIKSMIESDRAASNS PTQ
Sbjct: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSQPTQ 380

[Close]

BLAST of CmaCh11G018620 vs. ExPASy TrEMBL
Match: A0A1S4E162 (Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1)

HSP 1 Score: 699.5 bits (1804), Expect = 8.0e-198
Identity = 359/396 (90.66%), Postives = 365/396 (92.17%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           MN R  SLARI +S+ RS S SSSSTSASATSSQ PRTLAGLRERLAAESP+LSDFV L+
Sbjct: 1   MNKRCQSLARILKSVSRSFSSSSSSTSASATSSQFPRTLAGLRERLAAESPSLSDFVDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDPNEP N
Sbjct: 121 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPNEPTN 180

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGD G 
Sbjct: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDSGC 240

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKVA+SGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEF+PSGTLTKTSIMLG
Sbjct: 241 VEKVARSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFSPSGTLTKTSIMLG 300

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY NLGMKMGFR
Sbjct: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFR 360

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQ 397
           YVASGPMVRSSYKAGEFYIKSMIESDRAASNS PTQ
Sbjct: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSQPTQ 380

[Close]

BLAST of CmaCh11G018620 vs. ExPASy TrEMBL
Match: A0A6J1GL74 (Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455343 PE=3 SV=1)

HSP 1 Score: 692.2 bits (1785), Expect = 1.3e-195
Identity = 352/397 (88.66%), Postives = 365/397 (91.94%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           MN+   SLAR+ ES+GR  S SSSS +ASATSSQ PRTLAGLRERLA E+P+LSDF+ L+
Sbjct: 1   MNSHGRSLARLLESVGRFFSSSSSSAAASATSSQFPRTLAGLRERLAVETPSLSDFIDLQ 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SS+SYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 61  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTR                 SRFCNVKTSRTPPPPDPNEP+N
Sbjct: 121 SGGETGTATATIMILGDTCTR----------------VSRFCNVKTSRTPPPPDPNEPKN 180

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGDPG 
Sbjct: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGC 240

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VE VAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG
Sbjct: 241 VENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY NLGMKMGFR
Sbjct: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFR 360

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQP 398
           YVASGPMVRSSYKAGEFYIKSMIESDR+AS+SHPTQP
Sbjct: 361 YVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 381

[Close]

BLAST of CmaCh11G018620 vs. NCBI nr
Match: XP_022988813.1 (lipoyl synthase 2, mitochondrial [Cucurbita maxima])

HSP 1 Score: 746.5 bits (1926), Expect = 1.2e-211
Identity = 381/397 (95.97%), Postives = 381/397 (95.97%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK
Sbjct: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDPNEPQN
Sbjct: 121 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPNEPQN 180

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS
Sbjct: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG
Sbjct: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR
Sbjct: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQP 398
           YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQP
Sbjct: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQP 381

[Close]

BLAST of CmaCh11G018620 vs. NCBI nr
Match: XP_023531015.1 (lipoyl synthase 2, mitochondrial [Cucurbita pepo subsp. pepo])

HSP 1 Score: 739.6 bits (1908), Expect = 1.4e-209
Identity = 376/396 (94.95%), Postives = 378/396 (95.45%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           MNTRRHSLARIFES+GRSLSYSSSSTSASATSSQ+PRTLAGLRERLAAESPTLSDFV LK
Sbjct: 1   MNTRRHSLARIFESVGRSLSYSSSSTSASATSSQIPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDPNEPQN
Sbjct: 121 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPNEPQN 180

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGDPGS
Sbjct: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGS 240

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG
Sbjct: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR
Sbjct: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQ 397
           YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQ
Sbjct: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQ 380

[Close]

BLAST of CmaCh11G018620 vs. NCBI nr
Match: KAG7022903.1 (Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 738.0 bits (1904), Expect = 4.2e-209
Identity = 376/396 (94.95%), Postives = 377/396 (95.20%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           MN RRHSLARIFES+GRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFV LK
Sbjct: 1   MNARRHSLARIFESVGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDPNEPQN
Sbjct: 121 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPNEPQN 180

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGDPGS
Sbjct: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGS 240

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG
Sbjct: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR
Sbjct: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQ 397
           YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQ
Sbjct: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQ 380

[Close]

BLAST of CmaCh11G018620 vs. NCBI nr
Match: XP_022930787.1 (lipoyl synthase 2, mitochondrial-like [Cucurbita moschata])

HSP 1 Score: 736.1 bits (1899), Expect = 1.6e-208
Identity = 375/396 (94.70%), Postives = 377/396 (95.20%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           MN RRHSLARIFES+GRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFV LK
Sbjct: 1   MNARRHSLARIFESVGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVGLK 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW
Sbjct: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDPNEPQN
Sbjct: 121 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPNEPQN 180

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGDPGS
Sbjct: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGS 240

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG
Sbjct: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR
Sbjct: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQ 397
           YVASGPMVRSSYKAGEFYIKSMIESDRAAS+SHPTQ
Sbjct: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASSSHPTQ 380

[Close]

BLAST of CmaCh11G018620 vs. NCBI nr
Match: KAG7011959.1 (Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 704.1 bits (1816), Expect = 6.7e-199
Identity = 357/397 (89.92%), Postives = 370/397 (93.20%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           MN+   SLAR+ ES+GRS S SSSST+ASATSSQ  RTLAGLRERLA E+P+LSDF+ L+
Sbjct: 28  MNSHGRSLARLLESVGRSFSSSSSSTAASATSSQFSRTLAGLRERLAVETPSLSDFLDLQ 87

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
           SS+SYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEA+CPNLGECW
Sbjct: 88  SSESYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEARCPNLGECW 147

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGCRL             SRFCNVKTSRTPPPPDPNEP+N
Sbjct: 148 SGGETGTATATIMILGDTCTRGCRL-------------SRFCNVKTSRTPPPPDPNEPKN 207

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETV KLKVLKPNMLIEALVPDFRGDPG 
Sbjct: 208 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVQKLKVLKPNMLIEALVPDFRGDPGC 267

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VE VAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG
Sbjct: 268 VENVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 327

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKY NLGMKMGFR
Sbjct: 328 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYQNLGMKMGFR 387

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNSHPTQP 398
           YVASGPMVRSSYKAGEFYIKSMIESDR+AS+SHPTQP
Sbjct: 388 YVASGPMVRSSYKAGEFYIKSMIESDRSASSSHPTQP 411

[Close]

BLAST of CmaCh11G018620 vs. TAIR 10
Match: AT2G20860.1 (lipoic acid synthase 1 )

HSP 1 Score: 600.1 bits (1546), Expect = 1.3e-171
Identity = 306/392 (78.06%), Postives = 333/392 (84.95%), Query Frame = 0


Query: 1   MNTRRHSLARIFESIGRSLSYSSSSTSASATSSQVPRTLAGLRERLAAESPTLSDFVSLK 60
           M++R   L R      R   +SSSS     T +Q P++L  LR RLA ESP+L+DF+   
Sbjct: 1   MHSRSALLYRFLRPASR--CFSSSSAVTPVTVTQSPKSLEALRARLANESPSLTDFI--- 60

Query: 61  SSDSYSVEVGTKKKPLPKPKWMKESVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECW 120
             D+YSVEVGTKKKPLPKPKWMKES+PGGE+YVQIKKKLR+LKLHTVCEEAKCPNLGECW
Sbjct: 61  HGDTYSVEVGTKKKPLPKPKWMKESIPGGERYVQIKKKLRDLKLHTVCEEAKCPNLGECW 120

Query: 121 SGGETGTATATIMILGDTCTRGCRLNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQN 180
           SGGETGTATATIMILGDTCTRGC                RFCNVKTSRTPPPPDPNEP N
Sbjct: 121 SGGETGTATATIMILGDTCTRGC----------------RFCNVKTSRTPPPPDPNEPNN 180

Query: 181 VAEAIASWGLDYVVITSVDRDDLPDQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGS 240
           VAEAIASWG+DYVVITSVDRDDLPDQGSGHFAETV +LK LKP MLIEALVPDFRGD G 
Sbjct: 181 VAEAIASWGVDYVVITSVDRDDLPDQGSGHFAETVQRLKFLKPEMLIEALVPDFRGDGGC 240

Query: 241 VEKVAKSGLDVFAHNIETVEELQGAVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLG 300
           VEKV+KSGLDV AHNIETVEELQ  VRDHRANFKQSLDVL MAKE+AP+GTLTKTS+MLG
Sbjct: 241 VEKVSKSGLDVLAHNIETVEELQSFVRDHRANFKQSLDVLRMAKEYAPAGTLTKTSVMLG 300

Query: 301 CGETPDQVVQTMEKVRAAGVDVMTFGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFR 360
           CGETPDQVV+TMEKVRAAGVDVMTFGQYMRPSKRHMPV+EY+TP+AFE+Y  LGM+MGFR
Sbjct: 301 CGETPDQVVKTMEKVRAAGVDVMTFGQYMRPSKRHMPVAEYVTPDAFERYRLLGMEMGFR 360

Query: 361 YVASGPMVRSSYKAGEFYIKSMIESDRAASNS 393
           YVASGPMVRSSYKAGE+YIKSMIE+DR AS S
Sbjct: 361 YVASGPMVRSSYKAGEYYIKSMIEADRVASPS 371

[Close]

BLAST of CmaCh11G018620 vs. TAIR 10
Match: AT5G08415.1 (Radical SAM superfamily protein )

HSP 1 Score: 369.0 bits (946), Expect = 4.8e-102
Identity = 189/368 (51.36%), Postives = 249/368 (67.66%), Query Frame = 0


Query: 42  LRERLAAESPTLSDFVSLKSSDSYSVEVGTKKKPLP-----------------KPKWMKE 101
           LR +  + S  + D  SLK S    +E G K +P P                 KP W+++
Sbjct: 46  LRTKAVSLSSEMEDSSSLKKS-LMELE-GKKSEPYPGGMPKMGPFTGRDPNVKKPAWLRQ 105

Query: 102 SVPGGEKYVQIKKKLRELKLHTVCEEAKCPNLGECWSGGETGTATATIMILGDTCTRGCR 161
             P GE++ ++K+ L  L L+TVCEEA+CPN+GECW+GG  G ATATIM+LGDTCTRGC 
Sbjct: 106 KAPQGERFQEVKESLSRLNLNTVCEEAQCPNIGECWNGGGDGVATATIMVLGDTCTRGC- 165

Query: 162 LNSIHSLLFSDYLHSRFCNVKTSRTPPPPDPNEPQNVAEAIASWGLDYVVITSVDRDDLP 221
                          RFC VKTSR PPPPDP EP+N A+AIASWG+DY+VITSVDRDD+P
Sbjct: 166 ---------------RFCAVKTSRNPPPPDPMEPENTAKAIASWGVDYIVITSVDRDDIP 225

Query: 222 DQGSGHFAETVLKLKVLKPNMLIEALVPDFRGDPGSVEKVAKSGLDVFAHNIETVEELQG 281
           D GSGHFA+TV  +K  KP+++IE L  DFRGD  +V+ +  SGLDVFAHN+ETV+ LQ 
Sbjct: 226 DGGSGHFAQTVKAMKRHKPDIMIECLTSDFRGDLEAVDTLVHSGLDVFAHNVETVKRLQR 285

Query: 282 AVRDHRANFKQSLDVLVMAKEFAPSGTLTKTSIMLGCGETPDQVVQTMEKVRAAGVDVMT 341
            VRD RA ++QS+ VL  AK   P G +TKTSIMLG GET +++ + M  +RA  VD++T
Sbjct: 286 LVRDPRAGYEQSMSVLKHAKISKP-GMITKTSIMLGLGETDEELKEAMADLRAIDVDILT 345

Query: 342 FGQYMRPSKRHMPVSEYITPEAFEKYHNLGMKMGFRYVASGPMVRSSYKAGEFYIKSMIE 393
            GQY++P+  H+ V EY+TPE F+ +   G  +GFRYVASGP+VRSSY+AGE ++K+M++
Sbjct: 346 LGQYLQPTPLHLTVKEYVTPEKFDFWKTYGESIGFRYVASGPLVRSSYRAGELFVKTMVK 394

The following BLAST results are available for this feature:

BLAST of CmaCh11G018620 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3LSN5	5.7e-177	80.31	Lipoyl synthase 2, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-2 PE=2 SV=1	[more]
Q3LSN4	7.5e-177	80.31	Lipoyl synthase 1, mitochondrial OS=Pisum sativum OX=3888 GN=LIP1-1 PE=2 SV=1	[more]
B9H5L9	2.8e-176	83.96	Lipoyl synthase, mitochondrial OS=Populus trichocarpa OX=3694 GN=LIP1 PE=3 SV=1	[more]
B7FM45	2.3e-173	78.01	Lipoyl synthase, mitochondrial OS=Medicago truncatula OX=3880 GN=LIP1 PE=2 SV=1	[more]
A5CB81	8.6e-173	83.24	Lipoyl synthase, mitochondrial OS=Vitis vinifera OX=29760 GN=LIP1 PE=3 SV=1	[more]

BLAST of CmaCh11G018620 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JNE9	5.7e-212	95.97	Lipoyl synthase, mitochondrial OS=Cucurbita maxima OX=3661 GN=LOC111486046 PE=3 ...	[more]
A0A6J1ERM2	7.7e-209	94.70	Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111437162 PE=...	[more]
A0A5A7SKJ1	8.0e-198	90.66	Lipoyl synthase, mitochondrial OS=Cucumis melo var. makuwa OX=1194695 GN=LIP1 PE...	[more]
A0A1S4E162	8.0e-198	90.66	Lipoyl synthase, mitochondrial OS=Cucumis melo OX=3656 GN=LOC103495529 PE=3 SV=1	[more]
A0A6J1GL74	1.3e-195	88.66	Lipoyl synthase, mitochondrial OS=Cucurbita moschata OX=3662 GN=LOC111455343 PE=...	[more]

BLAST of CmaCh11G018620 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022988813.1	1.2e-211	95.97	lipoyl synthase 2, mitochondrial [Cucurbita maxima]	[more]
XP_023531015.1	1.4e-209	94.95	lipoyl synthase 2, mitochondrial [Cucurbita pepo subsp. pepo]	[more]
KAG7022903.1	4.2e-209	94.95	Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]
XP_022930787.1	1.6e-208	94.70	lipoyl synthase 2, mitochondrial-like [Cucurbita moschata]	[more]
KAG7011959.1	6.7e-199	89.92	Lipoyl synthase, mitochondrial, partial [Cucurbita argyrosperma subsp. argyrospe...	[more]

BLAST of CmaCh11G018620 vs. TAIR 10


Match Name	E-value	Identity	Description
AT2G20860.1	1.3e-171	78.06	lipoic acid synthase 1	[more]
AT5G08415.1	4.8e-102	51.36	Radical SAM superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006638	Elp3/MiaB/NifB	SMART	SM00729	MiaB	coord: 129..352 e-value: 0.002 score: 13.7
IPR007197	Radical SAM	PFAM	PF04055	Radical_SAM	coord: 160..314 e-value: 3.5E-8 score: 34.0
IPR007197	Radical SAM	PROSITE	PS51918	RADICAL_SAM	coord: 124..360 score: 24.615335
IPR013785	Aldolase-type TIM barrel	GENE3D	3.20.20.70	Aldolase class I	coord: 160..369 e-value: 2.4E-9 score: 38.6
IPR031691	Lipoyl synthase, N-terminal	PFAM	PF16881	LIAS_N	coord: 22..113 e-value: 1.9E-17 score: 63.6
IPR003698	Lipoyl synthase	PIRSF	PIRSF005963	Lipoyl_synth	coord: 20..396 e-value: 4.5E-139 score: 461.3
IPR003698	Lipoyl synthase	SFLD	SFLDF00271	lipoyl_synthase	coord: 70..382 e-value: 0.0 score: 410.9
IPR003698	Lipoyl synthase	PANTHER	PTHR10949	LIPOYL SYNTHASE	coord: 33..391
IPR003698	Lipoyl synthase	HAMAP	MF_00206	Lipoyl_synth	coord: 78..388 score: 124.878769
None	No IPR available	SFLD	SFLDG01058	lipoyl_synthase_like	coord: 70..382 e-value: 0.0 score: 410.9
None	No IPR available	PANTHER	PTHR10949:SF0	LIPOYL SYNTHASE, MITOCHONDRIAL	coord: 33..391
None	No IPR available	SUPERFAMILY	102114	Radical SAM enzymes	coord: 94..385
IPR027527	Lipoyl synthase, mitochondrial	HAMAP	MF_03128	Lipoyl_synth_plantM	coord: 22..391 score: 59.565144

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh11G018620.1	CmaCh11G018620.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0009107	lipoate biosynthetic process
biological_process	GO:0009249	protein lipoylation
cellular_component	GO:0005739	mitochondrion
molecular_function	GO:0051539	4 iron, 4 sulfur cluster binding
molecular_function	GO:0016992	lipoate synthase activity
molecular_function	GO:0102552	lipoyl synthase activity (acting on glycine-cleavage complex H protein
molecular_function	GO:0102553	lipoyl synthase activity (acting on pyruvate dehydrogenase E2 protein)
molecular_function	GO:0046872	metal ion binding
molecular_function	GO:0003824	catalytic activity
molecular_function	GO:0051536	iron-sulfur cluster binding