CmaCh11G011920 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh11G011920
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectinesterase
Location	Cma_Chr11: 7073950 .. 7076059 (+)
RNA-Seq Expression	CmaCh11G011920
Synteny	CmaCh11G011920

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCTTATGATGATGCTGCTGCTGAGAACAGGAAACGCTATGCCATCATCGGCGTCTCGTCGATGCTACTGGTTGCGATGGTGGTGGCGGTGACCGTCGGAGTTAACCTAAACCAAGATGGAACCAGCGATCCGGCCACCGGAAAGAAGGCTCATGAAATATCTTCTTCTATGAAAGCCATCAAAGCCATTTGCCGGCCTACTGATTACAAGCGGGAATGTGTTGATAGCCTTAAGGGGTCCGGAAAATCGGACCCCAAGGAATTGGTTCAGGTTGGGTTCAAGGCTGCCATGAAACTCATCCAAGCCGCTGCTAAGAAATCTCTCACCTTAAGACAGATTGAGAAAGACCCTAGAGCTTCCCAAGCCCTTGCTGGGTGTAAGGAGCTCATGGATTTTTCTGTTGACGAACTTAAATTCTCCATACAAAAGCTTGGCGCCTTTGACATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTTAGTGCCGTTATTACTTATCAGGTAATTCATATTGTCCGCTTTCAGCATAAGCTCTCAGTTGATTAAGAAGTTGATGGGTTATTGTTTTTATTAGGAAACTTGCTTGGATGGGTTTGCGAACTCGACAGGGGACGCATCAGAGAAGATGAAGAAGGCATTGAAGATATCAATGCGATTGAGCAGCAATGGGTTGGCGATGGTGACGGAGTTGTCCTCCATGTTGACGGAGTTGCAAATTCCAGGGATCAACCGACGACTTCTCAGCATTCCAGTTCTCGGACACGAGGATTACCCCGAGTGGGCCGGCCCCGGTATGCAGCGTCTCCTCGCCGGTTCTCCAGCGAAGAGGAAGCCAAACATTGTGGTGGCTAAGGATGGAAGTGGGCAGTACAAAACCATTCAAGAGGCTATAAACAAAGTTCCCAAGAGGAAGAACAACGCAACTTTTGTAATCCATATCAAGCAAGGAGTGTACAAAGAATACGTTGTGATTGGAAAAAAGCTAACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATAATCTCGGGAAATAAGAACTTCGTCGATGGCACGACCACGTTCAAGACCGCTACCGTTGGTGGGTTAATAATGCATTTTTTTAGAAGATAGGGTTTGAACGATGTGGATCAATGATTTGTTGTTGTCGTCGTCGTTTGTTGTTTGCAGCTGTTTCCGCAGAGCACTTCATGGCTAGAGACATCGGATTCGAGAACACGGCAGGACCACATAAGCACCAAGCCGTAGCATTACGAGTCCAAGCAGACAAAGTAGTATTCTTCAACTGCGAGATGCATGGCTACCAAGACACCCTCTACGTACACACCATGCGTCAATTCTACCGTGATTGCACCATCTCCGGCACGATCGACTTCGTGTTTGGGGACGCAGCGGCCGTGTTCCAAAACTGCAGCTTCTTGGTGCGCAAGCCGCTCCCGAACCAACAAAACATCGTGACGGCACACGGGCGGAAGGAGCGGCGGCAGCCCTCGGCCCTGATCATCCAAAATTCAAAGTTCAAGCCAGATGCGGATCTGGTGCCAGTGAAGAAGCAGTTCCGGTCGTACCTAGGGCGGCCGTGGAAGGAATATTCTAGAACGATCATAATGGAATCATACATCGATGACATAATAGAGCCGGAAGGGTGGCTGCCGTGGGAGGGGGACTGGGGGCTGAGGACATGCTTTTACACGGAGTTCAACAACTACGGGCCGGGATCGGGGAAGTCGAAGAGGGTGAAGTGGAGGGGAATCAAGAGCATTACGGCGCAGCACGCGGTGGACTTTACTCCGGGGAGGTTCATATTGGGGGATAGATGGATTAAAGCCACCGGAATTCCGTACGTTTCAGGCATGACCAAACCCAACGCCGGAGTTGCTAATTGATTATATTATTAGTTTGATTTGTTAATGTAATTGTATTGTTCATGTAATTCTATTGGCGGCGGGCTGCCCTTTTCTTCTTCTTTTTCTTTTTCTCTCTCCAACGTGGTTTGAGTTGTGTGTGGGCCTAATTTTATATATTTTCTTTCTTTTAAAATTTAAATATTTTATTTATTTATTTATTTATTTATTTTGGAGTTGGGATTTGGAT

mRNA sequence

ATGGCTTATGATGATGCTGCTGCTGAGAACAGGAAACGCTATGCCATCATCGGCGTCTCGTCGATGCTACTGGTTGCGATGGTGGTGGCGGTGACCGTCGGAGTTAACCTAAACCAAGATGGAACCAGCGATCCGGCCACCGGAAAGAAGGCTCATGAAATATCTTCTTCTATGAAAGCCATCAAAGCCATTTGCCGGCCTACTGATTACAAGCGGGAATGTGTTGATAGCCTTAAGGGGTCCGGAAAATCGGACCCCAAGGAATTGGTTCAGGTTGGGTTCAAGGCTGCCATGAAACTCATCCAAGCCGCTGCTAAGAAATCTCTCACCTTAAGACAGATTGAGAAAGACCCTAGAGCTTCCCAAGCCCTTGCTGGGTGTAAGGAGCTCATGGATTTTTCTGTTGACGAACTTAAATTCTCCATACAAAAGCTTGGCGCCTTTGACATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTTAGTGCCGTTATTACTTATCAGGAAACTTGCTTGGATGGGTTTGCGAACTCGACAGGGGACGCATCAGAGAAGATGAAGAAGGCATTGAAGATATCAATGCGATTGAGCAGCAATGGGTTGGCGATGGTGACGGAGTTGTCCTCCATGTTGACGGAGTTGCAAATTCCAGGGATCAACCGACGACTTCTCAGCATTCCAGTTCTCGGACACGAGGATTACCCCGAGTGGGCCGGCCCCGGTATGCAGCGTCTCCTCGCCGGTTCTCCAGCGAAGAGGAAGCCAAACATTGTGGTGGCTAAGGATGGAAGTGGGCAGTACAAAACCATTCAAGAGGCTATAAACAAAGTTCCCAAGAGGAAGAACAACGCAACTTTTGTAATCCATATCAAGCAAGGAGTGTACAAAGAATACGTTGTGATTGGAAAAAAGCTAACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATAATCTCGGGAAATAAGAACTTCGTCGATGGCACGACCACGTTCAAGACCGCTACCGTTGCTGTTTCCGCAGAGCACTTCATGGCTAGAGACATCGGATTCGAGAACACGGCAGGACCACATAAGCACCAAGCCGTAGCATTACGAGTCCAAGCAGACAAAGTAGTATTCTTCAACTGCGAGATGCATGGCTACCAAGACACCCTCTACGTACACACCATGCGTCAATTCTACCGTGATTGCACCATCTCCGGCACGATCGACTTCGTGTTTGGGGACGCAGCGGCCGTGTTCCAAAACTGCAGCTTCTTGGTGCGCAAGCCGCTCCCGAACCAACAAAACATCGTGACGGCACACGGGCGGAAGGAGCGGCGGCAGCCCTCGGCCCTGATCATCCAAAATTCAAAGTTCAAGCCAGATGCGGATCTGGTGCCAGTGAAGAAGCAGTTCCGGTCGTACCTAGGGCGGCCGTGGAAGGAATATTCTAGAACGATCATAATGGAATCATACATCGATGACATAATAGAGCCGGAAGGGTGGCTGCCGTGGGAGGGGGACTGGGGGCTGAGGACATGCTTTTACACGGAGTTCAACAACTACGGGCCGGGATCGGGGAAGTCGAAGAGGGTGAAGTGGAGGGGAATCAAGAGCATTACGGCGCAGCACGCGGTGGACTTTACTCCGGGGAGGTTCATATTGGGGGATAGATGGATTAAAGCCACCGGAATTCCGTACGTTTCAGGCATGACCAAACCCAACGCCGGAGTTGCTAATTGATTATATTATTAGTTTGATTTGTTAATGTAATTGTATTGTTCATGTAATTCTATTGGCGGCGGGCTGCCCTTTTCTTCTTCTTTTTCTTTTTCTCTCTCCAACGTGGTTTGAGTTGTGTGTGGGCCTAATTTTATATATTTTCTTTCTTTTAAAATTTAAATATTTTATTTATTTATTTATTTATTTATTTTGGAGTTGGGATTTGGAT

Coding sequence (CDS)

ATGGCTTATGATGATGCTGCTGCTGAGAACAGGAAACGCTATGCCATCATCGGCGTCTCGTCGATGCTACTGGTTGCGATGGTGGTGGCGGTGACCGTCGGAGTTAACCTAAACCAAGATGGAACCAGCGATCCGGCCACCGGAAAGAAGGCTCATGAAATATCTTCTTCTATGAAAGCCATCAAAGCCATTTGCCGGCCTACTGATTACAAGCGGGAATGTGTTGATAGCCTTAAGGGGTCCGGAAAATCGGACCCCAAGGAATTGGTTCAGGTTGGGTTCAAGGCTGCCATGAAACTCATCCAAGCCGCTGCTAAGAAATCTCTCACCTTAAGACAGATTGAGAAAGACCCTAGAGCTTCCCAAGCCCTTGCTGGGTGTAAGGAGCTCATGGATTTTTCTGTTGACGAACTTAAATTCTCCATACAAAAGCTTGGCGCCTTTGACATTAGTAAACTCGATGAGATGTTGATCGATATTAAGATCTGGCTTAGTGCCGTTATTACTTATCAGGAAACTTGCTTGGATGGGTTTGCGAACTCGACAGGGGACGCATCAGAGAAGATGAAGAAGGCATTGAAGATATCAATGCGATTGAGCAGCAATGGGTTGGCGATGGTGACGGAGTTGTCCTCCATGTTGACGGAGTTGCAAATTCCAGGGATCAACCGACGACTTCTCAGCATTCCAGTTCTCGGACACGAGGATTACCCCGAGTGGGCCGGCCCCGGTATGCAGCGTCTCCTCGCCGGTTCTCCAGCGAAGAGGAAGCCAAACATTGTGGTGGCTAAGGATGGAAGTGGGCAGTACAAAACCATTCAAGAGGCTATAAACAAAGTTCCCAAGAGGAAGAACAACGCAACTTTTGTAATCCATATCAAGCAAGGAGTGTACAAAGAATACGTTGTGATTGGAAAAAAGCTAACCCATTTGATGCTCATTGGTGATGGCCCTAAAAAGACCATAATCTCGGGAAATAAGAACTTCGTCGATGGCACGACCACGTTCAAGACCGCTACCGTTGCTGTTTCCGCAGAGCACTTCATGGCTAGAGACATCGGATTCGAGAACACGGCAGGACCACATAAGCACCAAGCCGTAGCATTACGAGTCCAAGCAGACAAAGTAGTATTCTTCAACTGCGAGATGCATGGCTACCAAGACACCCTCTACGTACACACCATGCGTCAATTCTACCGTGATTGCACCATCTCCGGCACGATCGACTTCGTGTTTGGGGACGCAGCGGCCGTGTTCCAAAACTGCAGCTTCTTGGTGCGCAAGCCGCTCCCGAACCAACAAAACATCGTGACGGCACACGGGCGGAAGGAGCGGCGGCAGCCCTCGGCCCTGATCATCCAAAATTCAAAGTTCAAGCCAGATGCGGATCTGGTGCCAGTGAAGAAGCAGTTCCGGTCGTACCTAGGGCGGCCGTGGAAGGAATATTCTAGAACGATCATAATGGAATCATACATCGATGACATAATAGAGCCGGAAGGGTGGCTGCCGTGGGAGGGGGACTGGGGGCTGAGGACATGCTTTTACACGGAGTTCAACAACTACGGGCCGGGATCGGGGAAGTCGAAGAGGGTGAAGTGGAGGGGAATCAAGAGCATTACGGCGCAGCACGCGGTGGACTTTACTCCGGGGAGGTTCATATTGGGGGATAGATGGATTAAAGCCACCGGAATTCCGTACGTTTCAGGCATGACCAAACCCAACGCCGGAGTTGCTAATTGA

Protein sequence

MAYDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKAIKAICRPTDYKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDPRASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGINRRLLSIPVLGHEDYPEWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRPWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIKSITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTKPNAGVAN

Homology

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BLAST of CmaCh11G011920 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 645.6 bits (1664), Expect = 5.4e-184
Identity = 315/576 (54.69%), Postives = 425/576 (73.78%), Query Frame = 0


Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKAHEISSSMKAI 62
           YDD  A+ +KRY II +SS+LL++MVVAVT+GV++N+ D   D        EI++S+KAI
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD-------EEITTSVKAI 64

Query: 63  KAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDPR 122
           K +C PTDYK  C D+L+   K  SDP ELV+  F A MK I   AKKS T+ +++KDPR
Sbjct: 65  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 124

Query: 123 ASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
           A  AL  CKELMD+++ EL  S ++LG F+  K+DE L+ ++IWLSA I++++TCLDGF 
Sbjct: 125 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 184

Query: 183 NSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDYP 242
            + G+A E +KKALK +++L+ NGLAMVTE+S+ L ++QIP +N RRLLS      +++P
Sbjct: 185 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 244

Query: 243 EWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVY 302
            W     +RLL    ++ KP+IVVA+DGSGQYKTI EA+N VPK+K N TFV+HIK+G+Y
Sbjct: 245 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKEGIY 304

Query: 303 KEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFENT 362
           KEYV + + +THL+ IGDGP KT+ISG+K++ DG TT+KTATVA+  +HF+A++I FENT
Sbjct: 305 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 364

Query: 363 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 422
           AG  KHQAVA+RV AD+ +F+NC+  GYQDTLY H+ RQFYRDCTISGTIDF+FGDAAAV
Sbjct: 365 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 424

Query: 423 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 482
           FQNC+ LVRKPL NQ   +TAHGRK+ R+ +  ++Q      + D + VK+Q ++YLGRP
Sbjct: 425 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 484

Query: 483 WKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIKS 542
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+  +KRV W GIK 
Sbjct: 485 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 544

Query: 543 ITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTKPN 575
           ++ +  + FTP ++I GD WI   G+PY+ G+   N
Sbjct: 545 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGLFSGN 566

[Close]

BLAST of CmaCh11G011920 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 632.9 bits (1631), Expect = 3.6e-180
Identity = 313/582 (53.78%), Postives = 426/582 (73.20%), Query Frame = 0


Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKAHEISSSMKAI 62
           YDD  ++ ++RY +I +SS+LL++MVVAVTVGV+LN+ DG S     K   E+++S+KA+
Sbjct: 5   YDD-ESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDS-----KGKAEVNASVKAV 64

Query: 63  KAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDPR 122
           K +C PTDY++ C D+L  +GK  +DP ELV+  F   MK I  AAKKS T+ +++KD R
Sbjct: 65  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 124

Query: 123 ASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
              AL  CKELMD+++DEL  S ++LG F+   LDE LI+++IWLSA I+++ETCL+GF 
Sbjct: 125 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 184

Query: 183 NSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDYP 242
            + G+A E MKKALK ++ L+ NGLA+++E+S+ + ++QIPG+N RRLL+      E +P
Sbjct: 185 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 244

Query: 243 EWAGPGMQRLLAGSPAKR--KPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQG 302
            W     ++LL  + A    KP+IVVA+DGSGQYKTI EA+  VPK K N TFV+HIK G
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 304

Query: 303 VYKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFE 362
           +YKEYV + K ++HL+ IGDGP KTIISGNKN+ DG TT++TATVA+   +F+A++IGFE
Sbjct: 305 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 364

Query: 363 NTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAA 422
           NTAG  KHQAVA+RVQ+D+ +FFNC   GYQDTLY H+ RQF+RDCTISGTIDF+FGDAA
Sbjct: 365 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 424

Query: 423 AVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLG 482
           AVFQNC+ LVRKPLPNQ   +TAHGRK+ R+ +  + Q      + D + VK+  ++YLG
Sbjct: 425 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 484

Query: 483 RPWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGI 542
           RPWKEYSRTIIM ++I D ++P+GW PW GD+GL+T FY+E  N GPGS  + RV W GI
Sbjct: 485 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 544

Query: 543 KSITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTKPNAGVA 579
           K+++ +  + FTP ++I GD WI   G+PY +G+   N   A
Sbjct: 545 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPAAA 573

[Close]

BLAST of CmaCh11G011920 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 513.1 bits (1320), Expect = 4.2e-144
Identity = 258/569 (45.34%), Postives = 372/569 (65.38%), Query Frame = 0


Query: 11  RKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKAIKAICRPTDY 70
           +K+  I GV + LLV MVVAV +  + N   +          +I ++  A++A+C PTDY
Sbjct: 9   KKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIV----PVQIKTATTAVEAVCAPTDY 68

Query: 71  KRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSLT--LRQIEKDPRASQALAG 130
           K  CV+SL  +    + P +L+++GF   ++ I+ + KK+      +   D     AL  
Sbjct: 69  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 128

Query: 131 CKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANSTGDAS 190
           C++LM+ + D+LK  +     F I ++++ + D+++WLS  I YQ+TC+D F  +    S
Sbjct: 129 CEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLS 188

Query: 191 EKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-------RRLLSIPVLGHEDYPE 250
           + M+K  K S  L+SNGLAM+T +S++L E  + G+        R+LLS      +  P 
Sbjct: 189 QDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS----AEDGIPS 248

Query: 251 WAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYK 310
           W GP  +RL+A +    K N+VVA DGSGQYKTI EA+N VPK  N   FVI+IKQGVY 
Sbjct: 249 WVGPNTRRLMA-TKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIYIKQGVYN 308

Query: 311 EYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDG-TTTFKTATVAVSAEHFMARDIGFENT 370
           E V + KK+TH+  IGDGP KT I+G+ N+  G   T+ TATVA++ ++F A++IGFENT
Sbjct: 309 EKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENT 368

Query: 371 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 430
           AGP  HQAVALRV AD  VF+NC++ GYQDTLYVH+ RQF+RDCT+SGT+DF+FGD   V
Sbjct: 369 AGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVV 428

Query: 431 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 490
            QNC+ +VRKP+ +Q  ++TA GR ++R+ + L++QN     +   +PVK   ++YLGRP
Sbjct: 429 LQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRP 488

Query: 491 WKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIKS 550
           WKE+SRTIIM + IDD+I+P GWLPW GD+ L T +Y E+ N GPGS +++RVKW GIK 
Sbjct: 489 WKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKK 548

Query: 551 ITAQHAVDFTPGRFILGDRWIKATGIPYV 568
           ++ + A+ FTP RF+ G+ WI    +PY+
Sbjct: 549 LSPKQALRFTPARFLRGNLWIPPNRVPYM 567

[Close]

BLAST of CmaCh11G011920 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 492.7 bits (1267), Expect = 5.9e-138
Identity = 252/575 (43.83%), Postives = 363/575 (63.13%), Query Frame = 0


Query: 9   ENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKAIKAICRPT 68
           + +K++ + G  S  LV MVV+V V        TS  +     + I  + KA++A+C PT
Sbjct: 6   DKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCAPT 65

Query: 69  DYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSL--TLRQIEKDPRASQAL 128
           D+K  CV+SL G+     DP +L+++GFK  +K I  + +K+      + +K+P A  A 
Sbjct: 66  DFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAF 125

Query: 129 AGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANSTGD 188
             C++LM  ++D+LK  +     F + +++  + D+++WLS  I +Q+TC+D F     +
Sbjct: 126 ELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSN 185

Query: 189 ASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-------RRLLSIPVLGHEDY 248
             + M K  K S  LSSN LAMVT +S+++    + G+        R+LLS      +  
Sbjct: 186 LMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----EDSI 245

Query: 249 PEWAGPGMQRLLA---GSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIK 308
           P W GP  +RL+A   G P   K N VVA+DG+GQ+KTI +A+N VPK  N   F+IHIK
Sbjct: 246 PTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIHIK 305

Query: 309 QGVYKEYVVIGKKLTHLMLIGDGPKKTIISGNKNF-VDGTTTFKTATVAVSAEHFMARDI 368
           +G+YKE V + KK+ H+  IGDGP KT+I+G+ NF +    TF TAT+ +  +HF A++I
Sbjct: 306 EGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNI 365

Query: 369 GFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFG 428
           G ENTAGP   QAVALRV AD  VF +C++ G+QDTLYVH+ RQFYRDCT+SGT+DF+FG
Sbjct: 366 GIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFG 425

Query: 429 DAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRS 488
           DA  + QNC  +VRKP   Q  +VTA GR   R+ + L++       D   +P+K   ++
Sbjct: 426 DAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKA 485

Query: 489 YLGRPWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKW 548
           YLGRPWKE+SRTIIM++ IDD+I+P GWLPW GD+ L+T +Y E  N GPGS +++RVKW
Sbjct: 486 YLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKW 545

Query: 549 RGIKSITAQHAVDFTPGRFILGDRWIKATGIPYVS 569
            GIK +T Q A+ +T  RF+ GD WI  T +PY +
Sbjct: 546 PGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of CmaCh11G011920 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 462.6 bits (1189), Expect = 6.5e-129
Identity = 268/703 (38.12%), Postives = 375/703 (53.34%), Query Frame = 0


Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSM---KAIKAICRPTD 72
           +Y ++GV++ L++AMV+ V        DG S     ++ H  S  M     +  IC  TD
Sbjct: 3   KYVLLGVTA-LIMAMVICVEA-----NDGNSPSRKMEEVHRESKLMITKTTVSIICASTD 62

Query: 73  YKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSL--TLRQIEKDPRASQALAGC 132
           YK++C  SL      DP+ L++  F  A+  I++   + +     + + D    +AL  C
Sbjct: 63  YKQDCTTSLATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTC 122

Query: 133 KELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANSTGDASE 192
           +ELMD ++D+L+ +  K   F  ++L + + D+ +WLS  ITYQ+TC+DGF     +A+ 
Sbjct: 123 RELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAV 182

Query: 193 KMKKALKISMRLSSNGLAMVTELSSMLTELQI--PGINRRLLSIPVLG------------ 252
            M++ ++    L+SNGLA+   L  +L   +I  P +  R   + +LG            
Sbjct: 183 MMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSS 242

Query: 253 -----HED---------------------------------------------------- 312
                +ED                                                    
Sbjct: 243 QDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKS 302

Query: 313 ------------------------------------------------------------ 372
                                                                       
Sbjct: 303 AFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSL 362

Query: 373 ----------YPEWAGPGMQRLLAGSPAKR--KPNIVVAKDGSGQYKTIQEAINKVPKRK 432
                     +P W  P  +RLLA  P     K N+VVAKDGSG+ KTI +A+  VP  K
Sbjct: 363 KDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-MK 422

Query: 433 NNATFVIHIKQGVYKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFV-DGTTTFKTATVAV 492
           N   FVIHIK+GVYKE V + KK+ H+M +GDGP KT+I+G+  F+ D   T++TA+VAV
Sbjct: 423 NTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAV 482

Query: 493 SAEHFMARDIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCT 552
           + ++FMA+DIGFENTAG  +HQAVALRV AD  VFFNC M+GYQDTLYVHT RQFYR+C 
Sbjct: 483 NGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCR 542

Query: 553 ISGTIDFVFGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDAD 567
           +SGTIDFVFGDA AVFQNC F++R+P+ +QQ IVTA GRK+RR+ + ++I NS+   DA 
Sbjct: 543 VSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDAS 602

[Close]

BLAST of CmaCh11G011920 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 645.6 bits (1664), Expect = 3.8e-185
Identity = 315/576 (54.69%), Postives = 425/576 (73.78%), Query Frame = 0


Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKAHEISSSMKAI 62
           YDD  A+ +KRY II +SS+LL++MVVAVT+GV++N+ D   D        EI++S+KAI
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAGD-------EEITTSVKAI 64

Query: 63  KAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDPR 122
           K +C PTDYK  C D+L+   K  SDP ELV+  F A MK I   AKKS T+ +++KDPR
Sbjct: 65  KDVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPR 124

Query: 123 ASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
           A  AL  CKELMD+++ EL  S ++LG F+  K+DE L+ ++IWLSA I++++TCLDGF 
Sbjct: 125 AKMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQ 184

Query: 183 NSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDYP 242
            + G+A E +KKALK +++L+ NGLAMVTE+S+ L ++QIP +N RRLLS      +++P
Sbjct: 185 GTQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS------QEFP 244

Query: 243 EWAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVY 302
            W     +RLL    ++ KP+IVVA+DGSGQYKTI EA+N VPK+K N TFV+HIK+G+Y
Sbjct: 245 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKK-NTTFVVHIKEGIY 304

Query: 303 KEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFENT 362
           KEYV + + +THL+ IGDGP KT+ISG+K++ DG TT+KTATVA+  +HF+A++I FENT
Sbjct: 305 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 364

Query: 363 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 422
           AG  KHQAVA+RV AD+ +F+NC+  GYQDTLY H+ RQFYRDCTISGTIDF+FGDAAAV
Sbjct: 365 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 424

Query: 423 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 482
           FQNC+ LVRKPL NQ   +TAHGRK+ R+ +  ++Q      + D + VK+Q ++YLGRP
Sbjct: 425 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 484

Query: 483 WKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIKS 542
           WKEYSRTIIM ++I D + PEGW PW G++GL T FY+E  N GPG+  +KRV W GIK 
Sbjct: 485 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 544

Query: 543 ITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTKPN 575
           ++ +  + FTP ++I GD WI   G+PY+ G+   N
Sbjct: 545 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGLFSGN 566

[Close]

BLAST of CmaCh11G011920 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 632.9 bits (1631), Expect = 2.6e-181
Identity = 313/582 (53.78%), Postives = 426/582 (73.20%), Query Frame = 0


Query: 3   YDDAAAENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQ-DGTSDPATGKKAHEISSSMKAI 62
           YDD  ++ ++RY +I +SS+LL++MVVAVTVGV+LN+ DG S     K   E+++S+KA+
Sbjct: 5   YDD-ESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHDGDS-----KGKAEVNASVKAV 64

Query: 63  KAICRPTDYKRECVDSLKGSGK--SDPKELVQVGFKAAMKLIQAAAKKSLTLRQIEKDPR 122
           K +C PTDY++ C D+L  +GK  +DP ELV+  F   MK I  AAKKS T+ +++KD R
Sbjct: 65  KDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKDSR 124

Query: 123 ASQALAGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFA 182
              AL  CKELMD+++DEL  S ++LG F+   LDE LI+++IWLSA I+++ETCL+GF 
Sbjct: 125 TRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEGFQ 184

Query: 183 NSTGDASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-RRLLSIPVLGHEDYP 242
            + G+A E MKKALK ++ L+ NGLA+++E+S+ + ++QIPG+N RRLL+      E +P
Sbjct: 185 GTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLLA------EGFP 244

Query: 243 EWAGPGMQRLLAGSPAKR--KPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQG 302
            W     ++LL  + A    KP+IVVA+DGSGQYKTI EA+  VPK K N TFV+HIK G
Sbjct: 245 SWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHIKAG 304

Query: 303 VYKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDGTTTFKTATVAVSAEHFMARDIGFE 362
           +YKEYV + K ++HL+ IGDGP KTIISGNKN+ DG TT++TATVA+   +F+A++IGFE
Sbjct: 305 LYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNIGFE 364

Query: 363 NTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAA 422
           NTAG  KHQAVA+RVQ+D+ +FFNC   GYQDTLY H+ RQF+RDCTISGTIDF+FGDAA
Sbjct: 365 NTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFGDAA 424

Query: 423 AVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLG 482
           AVFQNC+ LVRKPLPNQ   +TAHGRK+ R+ +  + Q      + D + VK+  ++YLG
Sbjct: 425 AVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKAYLG 484

Query: 483 RPWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGI 542
           RPWKEYSRTIIM ++I D ++P+GW PW GD+GL+T FY+E  N GPGS  + RV W GI
Sbjct: 485 RPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTWAGI 544

Query: 543 KSITAQHAVDFTPGRFILGDRWIKATGIPYVSGMTKPNAGVA 579
           K+++ +  + FTP ++I GD WI   G+PY +G+   N   A
Sbjct: 545 KTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGLLAGNPAAA 573

[Close]

BLAST of CmaCh11G011920 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 513.1 bits (1320), Expect = 3.0e-145
Identity = 258/569 (45.34%), Postives = 372/569 (65.38%), Query Frame = 0


Query: 11  RKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKAIKAICRPTDY 70
           +K+  I GV + LLV MVVAV +  + N   +          +I ++  A++A+C PTDY
Sbjct: 9   KKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIV----PVQIKTATTAVEAVCAPTDY 68

Query: 71  KRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSLT--LRQIEKDPRASQALAG 130
           K  CV+SL  +    + P +L+++GF   ++ I+ + KK+      +   D     AL  
Sbjct: 69  KETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKDTKGALEL 128

Query: 131 CKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANSTGDAS 190
           C++LM+ + D+LK  +     F I ++++ + D+++WLS  I YQ+TC+D F  +    S
Sbjct: 129 CEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFEETNSKLS 188

Query: 191 EKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-------RRLLSIPVLGHEDYPE 250
           + M+K  K S  L+SNGLAM+T +S++L E  + G+        R+LLS      +  P 
Sbjct: 189 QDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLS----AEDGIPS 248

Query: 251 WAGPGMQRLLAGSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIKQGVYK 310
           W GP  +RL+A +    K N+VVA DGSGQYKTI EA+N VPK  N   FVI+IKQGVY 
Sbjct: 249 WVGPNTRRLMA-TKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIYIKQGVYN 308

Query: 311 EYVVIGKKLTHLMLIGDGPKKTIISGNKNFVDG-TTTFKTATVAVSAEHFMARDIGFENT 370
           E V + KK+TH+  IGDGP KT I+G+ N+  G   T+ TATVA++ ++F A++IGFENT
Sbjct: 309 EKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAKNIGFENT 368

Query: 371 AGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAAV 430
           AGP  HQAVALRV AD  VF+NC++ GYQDTLYVH+ RQF+RDCT+SGT+DF+FGD   V
Sbjct: 369 AGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFIFGDGIVV 428

Query: 431 FQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRSYLGRP 490
            QNC+ +VRKP+ +Q  ++TA GR ++R+ + L++QN     +   +PVK   ++YLGRP
Sbjct: 429 LQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSINKAYLGRP 488

Query: 491 WKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKWRGIKS 550
           WKE+SRTIIM + IDD+I+P GWLPW GD+ L T +Y E+ N GPGS +++RVKW GIK 
Sbjct: 489 WKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRVKWPGIKK 548

Query: 551 ITAQHAVDFTPGRFILGDRWIKATGIPYV 568
           ++ + A+ FTP RF+ G+ WI    +PY+
Sbjct: 549 LSPKQALRFTPARFLRGNLWIPPNRVPYM 567

[Close]

BLAST of CmaCh11G011920 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 492.7 bits (1267), Expect = 4.2e-139
Identity = 252/575 (43.83%), Postives = 363/575 (63.13%), Query Frame = 0


Query: 9   ENRKRYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSMKAIKAICRPT 68
           + +K++ + G  S  LV MVV+V V        TS  +     + I  + KA++A+C PT
Sbjct: 6   DKKKKFIVAGSVSGFLVIMVVSVAV-------VTSKHSPKDDENHIRKTTKAVQAVCAPT 65

Query: 69  DYKRECVDSLKGSG--KSDPKELVQVGFKAAMKLIQAAAKKSL--TLRQIEKDPRASQAL 128
           D+K  CV+SL G+     DP +L+++GFK  +K I  + +K+      + +K+P A  A 
Sbjct: 66  DFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKGAF 125

Query: 129 AGCKELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANSTGD 188
             C++LM  ++D+LK  +     F + +++  + D+++WLS  I +Q+TC+D F     +
Sbjct: 126 ELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIKSN 185

Query: 189 ASEKMKKALKISMRLSSNGLAMVTELSSMLTELQIPGIN-------RRLLSIPVLGHEDY 248
             + M K  K S  LSSN LAMVT +S+++    + G+        R+LLS      +  
Sbjct: 186 LMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLST----EDSI 245

Query: 249 PEWAGPGMQRLLA---GSPAKRKPNIVVAKDGSGQYKTIQEAINKVPKRKNNATFVIHIK 308
           P W GP  +RL+A   G P   K N VVA+DG+GQ+KTI +A+N VPK  N   F+IHIK
Sbjct: 246 PTWVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIHIK 305

Query: 309 QGVYKEYVVIGKKLTHLMLIGDGPKKTIISGNKNF-VDGTTTFKTATVAVSAEHFMARDI 368
           +G+YKE V + KK+ H+  IGDGP KT+I+G+ NF +    TF TAT+ +  +HF A++I
Sbjct: 306 EGIYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNI 365

Query: 369 GFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFG 428
           G ENTAGP   QAVALRV AD  VF +C++ G+QDTLYVH+ RQFYRDCT+SGT+DF+FG
Sbjct: 366 GIENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFG 425

Query: 429 DAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDADLVPVKKQFRS 488
           DA  + QNC  +VRKP   Q  +VTA GR   R+ + L++       D   +P+K   ++
Sbjct: 426 DAKCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKA 485

Query: 489 YLGRPWKEYSRTIIMESYIDDIIEPEGWLPWEGDWGLRTCFYTEFNNYGPGSGKSKRVKW 548
           YLGRPWKE+SRTIIM++ IDD+I+P GWLPW GD+ L+T +Y E  N GPGS +++RVKW
Sbjct: 486 YLGRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKW 545

Query: 549 RGIKSITAQHAVDFTPGRFILGDRWIKATGIPYVS 569
            GIK +T Q A+ +T  RF+ GD WI  T +PY +
Sbjct: 546 PGIKKLTPQDALLYTGDRFLRGDTWIPQTQVPYTA 566

[Close]

BLAST of CmaCh11G011920 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 462.6 bits (1189), Expect = 4.6e-130
Identity = 268/703 (38.12%), Postives = 375/703 (53.34%), Query Frame = 0


Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNQDGTSDPATGKKAHEISSSM---KAIKAICRPTD 72
           +Y ++GV++ L++AMV+ V        DG S     ++ H  S  M     +  IC  TD
Sbjct: 3   KYVLLGVTA-LIMAMVICVEA-----NDGNSPSRKMEEVHRESKLMITKTTVSIICASTD 62

Query: 73  YKRECVDSLKGSGKSDPKELVQVGFKAAMKLIQAAAKKSL--TLRQIEKDPRASQALAGC 132
           YK++C  SL      DP+ L++  F  A+  I++   + +     + + D    +AL  C
Sbjct: 63  YKQDCTTSLATVRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTC 122

Query: 133 KELMDFSVDELKFSIQKLGAFDISKLDEMLIDIKIWLSAVITYQETCLDGFANSTGDASE 192
           +ELMD ++D+L+ +  K   F  ++L + + D+ +WLS  ITYQ+TC+DGF     +A+ 
Sbjct: 123 RELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAV 182

Query: 193 KMKKALKISMRLSSNGLAMVTELSSMLTELQI--PGINRRLLSIPVLG------------ 252
            M++ ++    L+SNGLA+   L  +L   +I  P +  R   + +LG            
Sbjct: 183 MMERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSS 242

Query: 253 -----HED---------------------------------------------------- 312
                +ED                                                    
Sbjct: 243 QDSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKS 302

Query: 313 ------------------------------------------------------------ 372
                                                                       
Sbjct: 303 AFSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSL 362

Query: 373 ----------YPEWAGPGMQRLLAGSPAKR--KPNIVVAKDGSGQYKTIQEAINKVPKRK 432
                     +P W  P  +RLLA  P     K N+VVAKDGSG+ KTI +A+  VP  K
Sbjct: 363 KDRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-MK 422

Query: 433 NNATFVIHIKQGVYKEYVVIGKKLTHLMLIGDGPKKTIISGNKNFV-DGTTTFKTATVAV 492
           N   FVIHIK+GVYKE V + KK+ H+M +GDGP KT+I+G+  F+ D   T++TA+VAV
Sbjct: 423 NTKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAV 482

Query: 493 SAEHFMARDIGFENTAGPHKHQAVALRVQADKVVFFNCEMHGYQDTLYVHTMRQFYRDCT 552
           + ++FMA+DIGFENTAG  +HQAVALRV AD  VFFNC M+GYQDTLYVHT RQFYR+C 
Sbjct: 483 NGDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCR 542

Query: 553 ISGTIDFVFGDAAAVFQNCSFLVRKPLPNQQNIVTAHGRKERRQPSALIIQNSKFKPDAD 567
           +SGTIDFVFGDA AVFQNC F++R+P+ +QQ IVTA GRK+RR+ + ++I NS+   DA 
Sbjct: 543 VSGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDAS 602

The following BLAST results are available for this feature:

BLAST of CmaCh11G011920 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E8Z8	5.4e-184	54.69	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q8GX86	3.6e-180	53.78	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	4.2e-144	45.34	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]
Q8GXA1	5.9e-138	43.83	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3...	[more]
O23447	6.5e-129	38.12	Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CmaCh11G011920 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G27870.1	3.8e-185	54.69	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05610.1	2.6e-181	53.78	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	3.0e-145	45.34	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	4.2e-139	43.83	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	4.6e-130	38.12	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 56..206 e-value: 2.1E-35 score: 133.6
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 60..206 e-value: 4.3E-24 score: 85.6
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 57..210 e-value: 1.7E-21 score: 74.3
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 259..556 e-value: 8.1E-140 score: 464.7
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 56..211 e-value: 6.5E-33 score: 115.9
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 59..211
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 253..570 e-value: 9.1E-119 score: 398.5
None	No IPR available	PANTHER	PTHR31707:SF131	PECTINESTERASE	coord: 16..572
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 16..572
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 63..212 e-value: 1.66024E-40 score: 142.577
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 285..304
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 404..413
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 257..569

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh11G011920.1	CmaCh11G011920.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity