CmaCh11G011530 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh11G011530
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	tRNA (Guanosine(18)-2'-O)-methyltransferase
Location	Cma_Chr11: 6658431 .. 6661359 (-)
RNA-Seq Expression	CmaCh11G011530
Synteny	CmaCh11G011530

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ACCTAGGTGATTAAATTGAATTTAGATCTATAGTCCCAAATGTGCTCATCAATTGGAGCTCATTTGATTTTGACCTATAATTCATATTTCGAAAGGACGCCGGCACTGCATTTGTGGATGAGGAGGGTGTCTATCTCTGCTTCTACAGCTGTCTGAACCATCGAAGCTCTTCAACAAGCAGCCATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTCTTTCTACTTGGAAGCTGCAGTTCCCGAACAACTCAACTAATTCTCCCTTCTGTTTCCTTCCCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTGTACTCTTCTCTCACTTCCTCTCTTTCTCGCTCTCGGAGGCCATACAATTTCTTTTGCATTTCTTTCTTAAATCTGTTCTTGACTTTCCTTCTGTTTTGATTGCTTTATAGCTTCAAGAAACAACCCTAAGGCAGTTTTTACAAGATGTTTTTCCTCGACTTCGGCCGCCGCATCTTCAGTAGAAGTAACAGACGCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGCTGAATATCCGCACCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAATTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGAGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCAGTTTGTTTGGTGGTAGAGGGTTTGAGTGATTTCGGCAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTGCAAGAATGATTGAGGTGAAATTGTTTTCGAATACATTTGCCTTTTCCCCCTTGATTTTCCCAATGGAAAATTGTTTGGTTCAGGAATTCGACAGCTGCTTCTAGTCCATTGCGCCGGCGGAATTTCATTCCTCTACATAGTGTGGTTTTACTTCGTTGTTCAATGGAGCACTTGAATTCAATCAATTAGTCAATTTGCATATCTGGTTTTATATGATTAGGAGGTTAAATTGATGAAAATGCATTTGATTTCCATGGTAATTGTTAGATATGGGTGACAATGTGACACATTCTACTTGCTAATAATGACAGAAATTTCCATTGAATACATTTCATTCATGAGTCATAATCTTTCATTCCATGTTTCTTCTTCTGAGATTATTGCAATTTCAATGCAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTATAGAATAGTATCGACGCATCTTGGATCGAATGCGGTATTGATTCATTTTGTAAACTTTCTCCCTTCCACAATGTCCCATGAAGTAGCTTTACATTGAAATCAATTCTGTTCTTATATCGATTGTCTTATGCCAATTTTATTATTTTTCAACCTTTTTGAGCTTATCCTGACAGCTGAGTTGTGATTGTCCTGTTTTAGGTTTCTGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGGAATGAAAGTAGGTAATTATCTTCTTTGCTTCAATAATACCGAGTTTGATCAAAGTTTTAGTACACTTTTTTAAATCTCTGCTAGCTGGGAGATATCTGTTATATTTAGCAAACCATAACTTAGTCCATGTTTAATAAACTGGCCTACATTTTTGTTGTCCAGCAATTCTAATCCGCTCATCCTTTGATTAGTGAGTTTCGAGTTGTGAATATTGTCCGAATCAGGAGAAATTATACTTGTAGCTAGTTATGATATTTACAATTTATGTTAGTATCTGAGGTTTAGTTCTCTTCGGTCTCAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCACTATTCCTATGAATGGGATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGACTGGTAACTCTAAAGTCATGTTTGATGTTTTGAATTCGTTTACGCCTAAGTGGTAGTTCAACTAGTTGGGGCGCTACCAGGGGTGTTGTCTGGAAATATGTCGATTCGAGTCTTCCGGTGGGTTCGACACAAAGGATGTCAAAATGGACATATACTGTTTCTAGTACTGAATTGATGGTATGTTCTTTATCAAACTTTTCAGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCTCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCGGGTTTGGAGGTGTTGCTGTAGAATGGCAAGAGTGGTCTCTTGATGTGTGTAGCAAATGAGTATGAGAAGCAGAAACACACAGAAGAGCCCCCTTCAAAGTTGATCAAAACAGGTAGGCCTGGGATTAAATGGGGATCAGAAAATTTGGAAGCAGAAGAACAATTTCCCCATCTGAAGCATTTTTATCAGCCTTCTTCTTTCTGTGGTAAGCTTCAGCATTCAATTCACATGGCTCTATAATGTTTTACTGTGATTCTGTAATTGACTTGGAGAAACTTTTTAACTTAGTTGTTCATATCAGTTTTTCTTTTCTAATGCTCAAAGATGTCTGTGAAACGCAGTGTCTGGTAATCGAAAAATATGATGCTGAGTTATCTGAAATGCTAAAAGTGAAAATGTAAAAGAGATGGTAATGTGTGGATGATATTATGGATGTTCATATATGTTTCTACTCCTAAGTATTCACGATCACGAG

mRNA sequence

ACCTAGGTGATTAAATTGAATTTAGATCTATAGTCCCAAATGTGCTCATCAATTGGAGCTCATTTGATTTTGACCTATAATTCATATTTCGAAAGGACGCCGGCACTGCATTTGTGGATGAGGAGGGTGTCTATCTCTGCTTCTACAGCTGTCTGAACCATCGAAGCTCTTCAACAAGCAGCCATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTCTTTCTACTTGGAAGCTGCAGTTCCCGAACAACTCAACTAATTCTCCCTTCTGTTTCCTTCCCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTCTTCAAGAAACAACCCTAAGGCAGTTTTTACAAGATGTTTTTCCTCGACTTCGGCCGCCGCATCTTCAGTAGAAGTAACAGACGCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGCTGAATATCCGCACCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAATTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGAGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCAGTTTGTTTGGTGGTAGAGGGTTTGAGTGATTTCGGCAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTATAGAATAGTATCGACGCATCTTGGATCGAATGCGGTTTCTGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGGAATGAAAGTAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCACTATTCCTATGAATGGGATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGACTGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCTCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCGGGTTTGGAGGTGTTGCTGTAGAATGGCAAGAGTGGTCTCTTGATGTGTGTAGCAAATGAGTATGAGAAGCAGAAACACACAGAAGAGCCCCCTTCAAAGTTGATCAAAACAGGTAGGCCTGGGATTAAATGGGGATCAGAAAATTTGGAAGCAGAAGAACAATTTCCCCATCTGAAGCATTTTTATCAGCCTTCTTCTTTCTGTGGTAAGCTTCAGCATTCAATTCACATGGCTCTATAATGTTTTACTGTGATTCTGTAATTGACTTGGAGAAACTTTTTAACTTAGTTGTTCATATCAGTTTTTCTTTTCTAATGCTCAAAGATGTCTGTGAAACGCAGTGTCTGGTAATCGAAAAATATGATGCTGAGTTATCTGAAATGCTAAAAGTGAAAATGTAAAAGAGATGGTAATGTGTGGATGATATTATGGATGTTCATATATGTTTCTACTCCTAAGTATTCACGATCACGAG

Coding sequence (CDS)

ATGGCGTCCTGCAGAATCGCAGTCCGACCTTCACTCTTTCTACTTGGAAGCTGCAGTTCCCGAACAACTCAACTAATTCTCCCTTCTGTTTCCTTCCCCTCACCACCGCTTTCTTCCTACTCTCCATTCTTCACCGCCGTCTCAAACCATTCTTCAAGAAACAACCCTAAGGCAGTTTTTACAAGATGTTTTTCCTCGACTTCGGCCGCCGCATCTTCAGTAGAAGTAACAGACGCTGTAGGAAGTGACCTAGAACCGCCGGAGATTCATACACAAGACACGGTGGAGCACCTTCTCACACACTCCGATGACGTTGCCAGGTTGATGAAGATGGAGCGGAGGCCGATTAATTTAGATGGAGCTGAATATCCGCACCAAGGGCGATGGTTTCCGTACCTGGATAAGTTTAGCTGTGAAAATTCTTGGTTGAATAGTGGTGAGGTTTTGGACGCCTTGGATCCTCTCATATTGGACTCGAGGAAAGAGAGGTTCAAGAACGTCGTGCGACACAGGAGCTATTCAGTTTGTTTGGTGGTAGAGGGTTTGAGTGATTTCGGCAACGTCTCGGCGGCGTTTCGATCTGCCGATGCACTTGGATTTCAGTCGGTTCATGTGGTCTCTTGCGACGCTTCCAAAAGGTACAGAGAGAATCGTCATGTTAGCATGGGAGCAGAGAAATGGTTGGACATTGAACTTTGGAACTCAACCCCAGAATGTTTTAGAATTCTGAAATCTCGGGGTTATAGAATAGTATCGACGCATCTTGGATCGAATGCGGTTTCTGTTTATGATATGGATTGGTCGTGTCCAACTGCAATTGTGGTTGGGAATGAAAGTAGGGGAATAAGTGATGAAGCCCTGGAGCTATCAGATTTGCATTGCACTATTCCTATGAATGGGATGGTAGATTCCTTCAACGTATCAGTTGCTGCTGGCATCCTCATGCACCATGCTGTTTGCGATCGAACATCTCGACTGGGCTGCCATGGAGATCTCACATCAATAGAAAGGCAAATTCTACTGGCGGAGTTCTCACTACGGCATAACAAGAACGCAATCAGCGTTGCTCACGAGTATGCGAAGAGGAAGAGTAGTAGCACGAGCTCGGGTTTGGAGGTGTTGCTGTAG

Protein sequence

MASCRIAVRPSLFLLGSCSSRTTQLILPSVSFPSPPLSSYSPFFTAVSNHSSRNNPKAVFTRCFSSTSAAASSVEVTDAVGSDLEPPEIHTQDTVEHLLTHSDDVARLMKMERRPINLDGAEYPHQGRWFPYLDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYAKRKSSSTSSGLEVLL

Homology

[Close]

BLAST of CmaCh11G011530 vs. ExPASy Swiss-Prot
Match: O67577 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=224324 GN=trmH PE=1 SV=1)

HSP 1 Score: 112.8 bits (281), Expect = 8.2e-24
Identity = 56/174 (32.18%), Postives = 102/174 (58.62%), Query Frame = 0


Query: 152 LDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDAS 211
           ++ L+L+ R +R + V+  R   + +  + + +  N SA  R+ DA+G   ++    +  
Sbjct: 3   MEYLVLEKRLKRLREVLEKRQKDLIVFADNVKNEHNFSAIVRTCDAVGVLYLYYYHAE-G 62

Query: 212 KRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPT 271
           K+ + N  ++ G+ KW+ IE  ++  +     K+RG++IV+T L   +V+  ++D++ PT
Sbjct: 63  KKAKINEGITQGSHKWVFIEKVDNPVQKLLEFKNRGFQIVATWLSKESVNFREVDYTKPT 122

Query: 272 AIVVGNESRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSR 326
            +VVGNE +G+S E +E++D    IPM GM  S NVSVA GI+++ A   R  +
Sbjct: 123 VLVVGNELQGVSPEIVEIADKKIVIPMYGMAQSLNVSVATGIILYEAQRQREEK 175

[Close]

BLAST of CmaCh11G011530 vs. ExPASy Swiss-Prot
Match: P0AGJ3 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334 GN=trmH PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.9e-22
Identity = 64/188 (34.04%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 159 SRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 218
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 219 HVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNE 278
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 279 SRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSI 338
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 339 ERQILLAE 344
           E+Q LL E
Sbjct: 184 EQQRLLFE 189

[Close]

BLAST of CmaCh11G011530 vs. ExPASy Swiss-Prot
Match: P0AGJ2 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=83333 GN=trmH PE=1 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.9e-22
Identity = 64/188 (34.04%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 159 SRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 218
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 219 HVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNE 278
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 279 SRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSI 338
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 339 ERQILLAE 344
           E+Q LL E
Sbjct: 184 EQQRLLFE 189

[Close]

BLAST of CmaCh11G011530 vs. ExPASy Swiss-Prot
Match: P0AGJ4 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH PE=3 SV=1)

HSP 1 Score: 106.7 bits (265), Expect = 5.9e-22
Identity = 64/188 (34.04%), Postives = 100/188 (53.19%), Query Frame = 0


Query: 159 SRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYRENR 218
           +R  R   ++  R   + + +E +    NVSA  R+ADA+G   VH V      R R   
Sbjct: 4   TRYARICEMLARRQPDLTVCMEQVHKPHNVSAIIRTADAVGVHEVHAVW--PGSRMRTMA 63

Query: 219 HVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGNE 278
             + G+  W+ ++   +  +    LK +G +I++THL  NAV   ++D++ PT I++G E
Sbjct: 64  SAAAGSNSWVQVKTHRTIGDAVAHLKGQGMQILATHLSDNAVDFREIDYTRPTCILMGQE 123

Query: 279 SRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHAVCDRTSR---LGCHGDLTSI 338
             GI+ EAL L+D    IPM GMV S NVSVA+ ++++ A   R +    L  +  L   
Sbjct: 124 KTGITQEALALADQDIIIPMIGMVQSLNVSVASALILYEAQRQRQNAGMYLRENSMLPEA 183

Query: 339 ERQILLAE 344
           E+Q LL E
Sbjct: 184 EQQRLLFE 189

[Close]

BLAST of CmaCh11G011530 vs. ExPASy Swiss-Prot
Match: Q72GI1 (tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC BAA-163 / DSM 7039 / HB27) OX=262724 GN=trmH PE=1 SV=1)

HSP 1 Score: 104.8 bits (260), Expect = 2.2e-21
Identity = 56/161 (34.78%), Postives = 91/161 (56.52%), Query Frame = 0


Query: 158 DSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSVHVVSCDASKRYREN 217
           ++R+ R + V+R R   + +++E +    N+SA  R+ DA+G    H V  + +      
Sbjct: 6   EARRRRIEEVLRRRQPDLTVLLENVHKPHNLSAILRTCDAVGVLEAHAV--NPTGGVPTF 65

Query: 218 RHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVYDMDWSCPTAIVVGN 277
              S G+ KW+ + +     E FR LK RG+ + +T L  +A    ++D++ PTA++ G 
Sbjct: 66  NETSGGSHKWVYLRVHPDLHEAFRFLKERGFTVYATALREDARDFREVDYTKPTAVLFGA 125

Query: 278 ESRGISDEALELSDLHCTIPMNGMVDSFNVSVAAGILMHHA 319
           E  G+S+EAL L+D    IPM GMV S NVSVAA +++  A
Sbjct: 126 EKWGVSEEALALADGAIKIPMLGMVQSLNVSVAAAVILFEA 164

[Close]

BLAST of CmaCh11G011530 vs. TAIR 10
Match: AT5G15390.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 389.0 bits (998), Expect = 4.2e-108
Identity = 210/357 (58.82%), Postives = 262/357 (73.39%), Query Frame = 0


Query: 15  LGSCSSRTTQLILPSV-SFPSPPLSSYSPFFTAVSNHSSRNNPKAVFTRCFSSTSAAASS 74
           + SC S    LI  S+ SFP  PL + +   T +S  +   +P +V     S    A  +
Sbjct: 1   MNSCKS----LIRASISSFPFVPLPNPNFSITFISVRAF--SPLSVLHPNSSCIVTARRT 60

Query: 75  VEVTDAVGSDLEPPEIHTQDTVEHLL-THSDDVARLMKMERRPINLDGAEYPHQGRWFPY 134
                A+  +    E   +DTV+ LL T+  + + LMKMERR  +L   E   +G WFPY
Sbjct: 61  FHGAVALSPE-SLTEESPKDTVKGLLTTNRGEASSLMKMERR-CSLSNGEGDCRGSWFPY 120

Query: 135 LDKFSCENSWLNSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAF 194
            D+F C    L+S EVL+A+ P +++ R +RF+ VV +RSYSVCLVVEGLSDFGN+SAAF
Sbjct: 121 EDRFRCGEVHLSSREVLEAVSPHMMEERTDRFRRVVENRSYSVCLVVEGLSDFGNISAAF 180

Query: 195 RSADALGFQSVHVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVS 254
           RSADALG QSVHVVSCD+SKRY  NRHVSMGAEKWLDIE W++  ECF++LKSRGYRI +
Sbjct: 181 RSADALGIQSVHVVSCDSSKRYNGNRHVSMGAEKWLDIEFWDTPKECFKVLKSRGYRIAT 240

Query: 255 THLGSNAVSVYDMDWSCPTAIVVGNESRGISDEALELSDLHCTIPMNGMVDSFNVSVAAG 314
           THLG + VS+YDMDWSCPTAIVVGNE RGISDEALELSDL C+IPMNGMVDSFNVSVAAG
Sbjct: 241 THLGMDTVSIYDMDWSCPTAIVVGNEGRGISDEALELSDLRCSIPMNGMVDSFNVSVAAG 300

Query: 315 ILMHHAVCDRTSRLGCHGDLTSIERQILLAEFSLRHNKNAISVAHEYAKRKSSSTSS 370
           ILMHHAV DRT+RLG HGDL+  E++IL+AEFSLRH++++I +A+E+AKRK   ++S
Sbjct: 301 ILMHHAVSDRTTRLGSHGDLSEAEKEILMAEFSLRHSRSSICIAYEFAKRKQQHSTS 349

[Close]

BLAST of CmaCh11G011530 vs. TAIR 10
Match: AT2G19870.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 60.5 bits (145), Expect = 3.4e-09
Identity = 51/206 (24.76%), Postives = 90/206 (43.69%), Query Frame = 0


Query: 144 NSGEVLDALDPLILDSRKERFKNVVRHRSYSVCLVVEGLSDFGNVSAAFRSADALGFQSV 203
           + G VLDA  PL L   KE          YS+ + ++ ++D  N+ A  RSA   G   V
Sbjct: 388 HQGLVLDA-SPLELVKVKELDPISSEEEKYSLWVALDEVTDPQNLGAIIRSAYFFGATGV 447

Query: 204 HVVSCDASKRYRENRHVSMGAEKWLDIELWNSTPECFRILKSRGYRIVSTHLGSNAVSVY 263
            V + +++         S G+ + +++    +  +        G+R+V   +   AV++ 
Sbjct: 448 VVCAKNSAPLSAVVSKASAGSLEVMELRYCKNMMQFLEASAENGWRVVGGSVSPKAVALN 507

Query: 264 DMDWSCPTAIVVGNESRGISDEALELSDLHCT--IPMNG--------------------- 323
           ++    PT +V+GNE  G+      L +  CT  + ++G                     
Sbjct: 508 EVLPGSPTILVLGNEGTGLR----PLVERSCTDLVRISGNMPNEVAVTESDDAEGEGFRS 567

Query: 324 --MVDSFNVSVAAGILMHHAVCDRTS 325
              V+S NVSVAAG+ +HH + ++ S
Sbjct: 568 FLAVESLNVSVAAGLFLHHLIGNKAS 588

[Close]

BLAST of CmaCh11G011530 vs. TAIR 10
Match: AT4G38020.1 (tRNA/rRNA methyltransferase (SpoU) family protein )

HSP 1 Score: 46.6 bits (109), Expect = 5.1e-05
Identity = 40/152 (26.32%), Postives = 72/152 (47.37%), Query Frame = 0


Query: 177 LVVEGLSDFGNVSAAFRSADALGFQSVHVV--SCDA--SKRYRENRHVS----MGAEKWL 236
           LV++ + D GN+    RSA A  +    ++   CD    K  R +R  S    + +  W 
Sbjct: 199 LVLDSIQDPGNLGTLVRSAMAFNWDGAFLLPGCCDPYNDKALRASRGASFQLPIVSGNWN 258

Query: 237 DIELWNSTPECFRILKSRGYRIVSTH----LGSNAVSVYDMDWSCPTAIVVGNESRGISD 296
            ++L  +    F++    G+   +T     +   +V         P  +++G+E  G+S+
Sbjct: 259 HLKLLENE---FQMKLLAGHPATTTQKLKPVSKLSVEFAQSLAEKPLCLILGSEGNGLSE 318

Query: 297 EALELSDLHCTIPMNGMVDSFNVSVAAGILMH 317
           +A ++  L  +IPM G  +S NVSVA GI ++
Sbjct: 319 QARKVCVL-VSIPMAGDFESLNVSVAGGIFLY 346

The following BLAST results are available for this feature:

BLAST of CmaCh11G011530 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
O67577	8.2e-24	32.18	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Aquifex aeolicus (strain VF5) OX=...	[more]
P0AGJ3	5.9e-22	34.04	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli O157:H7 OX=83334...	[more]
P0AGJ2	5.9e-22	34.04	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Escherichia coli (strain K12) OX=...	[more]
P0AGJ4	5.9e-22	34.04	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Shigella flexneri OX=623 GN=trmH ...	[more]
Q72GI1	2.2e-21	34.78	tRNA (guanosine(18)-2'-O)-methyltransferase OS=Thermus thermophilus (strain ATCC...	[more]

BLAST of CmaCh11G011530 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G15390.1	4.2e-108	58.82	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT2G19870.1	3.4e-09	24.76	tRNA/rRNA methyltransferase (SpoU) family protein	[more]
AT4G38020.1	5.1e-05	26.32	tRNA/rRNA methyltransferase (SpoU) family protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR001537	tRNA/rRNA methyltransferase, SpoU type	PFAM	PF00588	SpoU_methylase	coord: 175..316 e-value: 2.4E-34 score: 118.5
IPR029026	tRNA (guanine-N1-)-methyltransferase, N-terminal	GENE3D	3.40.1280.10		coord: 151..358 e-value: 2.1E-58 score: 199.0
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	PANTHER	PTHR43453	RRNA METHYLASE-LIKE	coord: 1..367
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	HAMAP	MF_02060	tRNA_methyltr_TrmH	coord: 157..351 score: 26.785631
IPR033671	tRNA (guanosine(18)-2'-O)-methyltransferase	CDD	cd18092	SpoU-like_TrmH	coord: 159..322 e-value: 2.83255E-79 score: 238.521
None	No IPR available	PANTHER	PTHR43453:SF1	RRNA METHYLASE-LIKE PROTEIN	coord: 1..367
IPR029028	Alpha/beta knot methyltransferases	SUPERFAMILY	75217	alpha/beta knot	coord: 159..324

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh11G011530.1	CmaCh11G011530.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030488	tRNA methylation
biological_process	GO:0006396	RNA processing
molecular_function	GO:0008173	RNA methyltransferase activity
molecular_function	GO:0000049	tRNA binding
molecular_function	GO:0008168	methyltransferase activity
molecular_function	GO:0003723	RNA binding