CmaCh11G011260 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh11G011260
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Isoaspartyl peptidase/L-asparaginase
Location	Cma_Chr11: 6377037 .. 6380052 (+)
RNA-Seq Expression	CmaCh11G011260
Synteny	CmaCh11G011260

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

CATCGAACAAAGCAAGCCTTCTAATAATCTCACGTCATGTCATCAAAACGACTAAGCGGCTCTCTCAGCCTCCGTCATCGATTGAGTGGAGCAATTGCATGAACCCTCCCGTAAATCGTCGGCGATCGATCTTCTAAATTACCTAGGGTTGGTGGTTTGATCCATTGGCGCAGGACCTAAATGGGTTGGGCAATTGCTCTTCACGGCGGCGCCGGCGACATCCCTCTCTCTTTGTCACTGGACCGCCGCCAACCCCGCGAGGAAACCCTCCGTCGTTGCCTTCATATCGGCGTTCAAGCTCTCAAAGAGCAGAAACCTCCATTGGATGTTGTTGAACTTGTGGTGAATATTATTTACTCCCTCTCTCTGTTTTTCTACCACTCTGGTTTAATATAAAATTGGTTCGATTAAGTTTGTGTAATCTTAGAGTTCGTAACGATTTTGTCTATTGATTTGTTTGCATAACGATTTCTTTCATGATGTTGTTTAAAATTTGTAATGATTGATCTGTAATGTAAAAAGTTCCAGTAGGTTGTAATGGAATTTCTGTAAGTGTATATAGATGGATGAATTGCTTGGAGAACAAATGTATATTTCATTTTTGTAAGAAACTAAAATGAAATTTTCAATGACATACAAAAAATAAACAGGGACTCCAATAAAAAAAAACAAGATCATGAATTGAATTATAAATTCTCAAGCTAAGTACCCCATGAAACACCGCAACACTTTGGCTTTCTCCCTCAAAGAAGAATGCAAGAGAATTAGGTGGATTTCATAAGTTCATGTTTATGCAACTTAGATTTATATAATCTATAGGATTAACCTATATTCAGAGTCAAGTAAACCCTTTTGTTTGTGCAAAATGCTTGGTTAACTGAATACTTGGTTGCTCTGTAAGTACTTTAGTACAGATTATCTCGACTTGACGATCAATTCATGGTGTATATGGCTTCTTTTCCATGGTAAGATGGTCAGCTTTTGAACTGTAGGTTCTCTTACACTTTCCCGTGCTGTTGAATTTGTTCTTGTACAGGTACGAGAGCTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGCTCTGTCTTAACAACCAATGGCACTGTCGAAATGGAAGCTAGCATCATGGATGGTAAGAAGAGATGTGGAGCCGTTTCTGGGCTCACCACTGTTGTTAATGCCATATCTCTAGCAAGGCTTGTTATGGAGAAAACACCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGTTAGAATTTCTACCCCGTTCCTATGTACACAGATCGATGCTTTGTGATTCATTACTGTTTTATTTGTGCACCAATTCTTATCTGTTCAATTTTCTTAAAATTGTCAAGGGAGTGGAGACCACTGATCCAAGCTACTTTATCACCCCACAGAACATTGAAAGGCTAAAACAAGCTAAAGAGGCAAACAGTGTGCAGGTTTGCTTCTTATCTTTGTTGAGGATTGTTCGGAGATAGTCCCACGTTAGCTAATTTAGTGAATGATCATGGGTTTATAAGTAAGGAATACATCTCCATTGGTATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATTAGAGCTTATACTCAAAGTGGACAATATCATACCATTGTGGTGAGTCATTATTCCTAACATGGTATCAGAGTCATGCCCTTAATTTAGCCATGTCAATAGAATCCTCAAATATCGAACAAAGTTGAGAGCCTCGAAGGTGTAGTCAAAAGTGACTTATGTGTCAAATAAAGGGTGTACTTTGTTCGAGGGTTTTAGAGAAAAGTAGTCAACCTCGATTAAGGGGAGGCTGTTCGAGGGCTATATAGAACTCAGGGGCTCTATGGTGTACTTTATTCGAGATGAGGATTGTTGAAGATTGTTGGGAGAGAGTCCCACATTGGCTAATTTAGGGAATGACCATAGGTTTATAAGTAAGGAATACATCTCCGTTGATATGAGGCCTTTTGGGGAAGCCCAAAGCAAAGCCATTCGAGCTTATACTCAAAGTGGACAACATCATACCGTTGTAGAGAGTCGTTATTCCTAACAATCTTCTATCCCAAACATGACTTCGACTGCTCTCTTTATGAACCAAAAGTTTATAGGTGTAGATATCCGTCTAATAGGGTTGAAAAAATCACATTATGGAGTTTCCCTGAGATTTTTATGTTTTAGCATCGGTTGATTTCTATGGTACTTTTTGTTTCTGCACATTGATAGCTGTTGTCATGAACACTTATAGATTGATTATACTCAACCAAGTCCCCAAGCCACAGTACAAGAAACAGGGATTGGAAATGGTGACAGTCAAATCGGGACTGTTGGATGTGTGGCTGTTGATAACCATGGGAATTTAGCTGCTGCAACTTCCACTGGTGGATTAGTAAACAAAATGGTCGGTAGGATCGGCGACACTCCATTAATAGGGGCCGGGACGTATGCTAACCATCTCTGTGCAGTTTCTGCAACTGGAAAGGGCGAAGCCATCATACGTGGTACTGTGGCAAGGGATGTGGCAGCTCTAATGGAGTTCAAGGATCTATCTCTTGAAGCAGCAGCAGCCTGTGCTGTCGAAAGTGTTCCGAAAGGCAATGTTGGCTTGATTGCAGTATCGGCCTCCGGCGAAGTTGCTATGCCTTTCAACACGACTGGAATGTTCCGAGCTTGCGCAACTGAAGATGGTTATTCAGAGATTGCAATATGGCCTTCTAACATGACTGAATGAATGTTGTTGCCCCGAAGCCTCTGCTGAAGTTTGGTTATATCTTCAGCTGTCAGAGAAATCCTCTGTTGTTTGCCTTCCTAAATATTAAGAATAGCCTTCCGAAGGATCTTTTCGTTTTCATTTCTTTGGTTGGATTTCAATGGCCAGCTTTTCTTTAAACACCGTTAACAATATATATATGTTTTTTATTTTTGACCTAACTTTCAAAACCCAAGGCCAACTCATGTTCGTTTTCACGTCTGTTAAATGTCATTTTAAAATAGAACAACA

mRNA sequence

CATCGAACAAAGCAAGCCTTCTAATAATCTCACGTCATGTCATCAAAACGACTAAGCGGCTCTCTCAGCCTCCGTCATCGATTGAGTGGAGCAATTGCATGAACCCTCCCGTAAATCGTCGGCGATCGATCTTCTAAATTACCTAGGGTTGGTGGTTTGATCCATTGGCGCAGGACCTAAATGGGTTGGGCAATTGCTCTTCACGGCGGCGCCGGCGACATCCCTCTCTCTTTGTCACTGGACCGCCGCCAACCCCGCGAGGAAACCCTCCGTCGTTGCCTTCATATCGGCGTTCAAGCTCTCAAAGAGCAGAAACCTCCATTGGATGTTGTTGAACTTGTGGTACGAGAGCTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGCTCTGTCTTAACAACCAATGGCACTGTCGAAATGGAAGCTAGCATCATGGATGGTAAGAAGAGATGTGGAGCCGTTTCTGGGCTCACCACTGTTGTTAATGCCATATCTCTAGCAAGGCTTGTTATGGAGAAAACACCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGGAGTGGAGACCACTGATCCAAGCTACTTTATCACCCCACAGAACATTGAAAGGCTAAAACAAGCTAAAGAGGCAAACAGTGTGCAGATTGATTATACTCAACCAAGTCCCCAAGCCACAGTACAAGAAACAGGGATTGGAAATGGTGACAGTCAAATCGGGACTGTTGGATGTGTGGCTGTTGATAACCATGGGAATTTAGCTGCTGCAACTTCCACTGGTGGATTAGTAAACAAAATGGTCGGTAGGATCGGCGACACTCCATTAATAGGGGCCGGGACGTATGCTAACCATCTCTGTGCAGTTTCTGCAACTGGAAAGGGCGAAGCCATCATACGTGGTACTGTGGCAAGGGATGTGGCAGCTCTAATGGAGTTCAAGGATCTATCTCTTGAAGCAGCAGCAGCCTGTGCTGTCGAAAGTGTTCCGAAAGGCAATGTTGGCTTGATTGCAGTATCGGCCTCCGGCGAAGTTGCTATGCCTTTCAACACGACTGGAATGTTCCGAGCTTGCGCAACTGAAGATGGTTATTCAGAGATTGCAATATGGCCTTCTAACATGACTGAATGAATGTTGTTGCCCCGAAGCCTCTGCTGAAGTTTGGTTATATCTTCAGCTGTCAGAGAAATCCTCTGTTGTTTGCCTTCCTAAATATTAAGAATAGCCTTCCGAAGGATCTTTTCGTTTTCATTTCTTTGGTTGGATTTCAATGGCCAGCTTTTCTTTAAACACCGTTAACAATATATATATGTTTTTTATTTTTGACCTAACTTTCAAAACCCAAGGCCAACTCATGTTCGTTTTCACGTCTGTTAAATGTCATTTTAAAATAGAACAACA

Coding sequence (CDS)

ATGGGTTGGGCAATTGCTCTTCACGGCGGCGCCGGCGACATCCCTCTCTCTTTGTCACTGGACCGCCGCCAACCCCGCGAGGAAACCCTCCGTCGTTGCCTTCATATCGGCGTTCAAGCTCTCAAAGAGCAGAAACCTCCATTGGATGTTGTTGAACTTGTGGTACGAGAGCTCGAAAACTGTCCACATTTCAATGCAGGTAGGGGCTCTGTCTTAACAACCAATGGCACTGTCGAAATGGAAGCTAGCATCATGGATGGTAAGAAGAGATGTGGAGCCGTTTCTGGGCTCACCACTGTTGTTAATGCCATATCTCTAGCAAGGCTTGTTATGGAGAAAACACCTCATATATATCTAGCATTTGATGGAGCTGAGGCTTTTGCAAGGGAACAAGGAGTGGAGACCACTGATCCAAGCTACTTTATCACCCCACAGAACATTGAAAGGCTAAAACAAGCTAAAGAGGCAAACAGTGTGCAGATTGATTATACTCAACCAAGTCCCCAAGCCACAGTACAAGAAACAGGGATTGGAAATGGTGACAGTCAAATCGGGACTGTTGGATGTGTGGCTGTTGATAACCATGGGAATTTAGCTGCTGCAACTTCCACTGGTGGATTAGTAAACAAAATGGTCGGTAGGATCGGCGACACTCCATTAATAGGGGCCGGGACGTATGCTAACCATCTCTGTGCAGTTTCTGCAACTGGAAAGGGCGAAGCCATCATACGTGGTACTGTGGCAAGGGATGTGGCAGCTCTAATGGAGTTCAAGGATCTATCTCTTGAAGCAGCAGCAGCCTGTGCTGTCGAAAGTGTTCCGAAAGGCAATGTTGGCTTGATTGCAGTATCGGCCTCCGGCGAAGTTGCTATGCCTTTCAACACGACTGGAATGTTCCGAGCTTGCGCAACTGAAGATGGTTATTCAGAGATTGCAATATGGCCTTCTAACATGACTGAATGA

Protein sequence

MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELENCPHFNAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAISLARLVMEKTPHIYLAFDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGEAIIRGTVARDVAALMEFKDLSLEAAAACAVESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEIAIWPSNMTE

Homology

[Close]

BLAST of CmaCh11G011260 vs. ExPASy Swiss-Prot
Match: P50287 (Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08100 PE=1 SV=2)

HSP 1 Score: 475.7 bits (1223), Expect = 4.1e-133
Identity = 249/319 (78.06%), Postives = 271/319 (84.95%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELEN 60
           +GWAIALHGGAGDIP+ L  +RR PRE  LR CL +G+ ALK  KPPLDV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYL 120
            P FNAG+GSVLT  GTVEMEASIMDGK KRCGAVSGLTTVVN ISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETGIGN 180
           AFD AEAFAR  GVET D S+FITP+NI RLKQAKE N VQ+DYT PSP+          
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVP-----DNC 181

Query: 181 GDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKG 240
           GDSQIGTVGCVAVD+ GNLA+ATSTGG VNKMVGRIGDTP+IGAGTYANHLCA+SATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 EAIIRGTVARDVAALMEFKDLSLEAAAACAV-ESVPKGNVGLIAVSASGEVAMPFNTTGM 300
           E IIRGTVARDVAALME+K LSL  AAA  V +SVP+G+ GL+AVSA+GEV MPFNTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

Query: 301 FRACATEDGYSEIAIWPSN 318
           FRACA+EDGYSEIAIWP+N
Sbjct: 302 FRACASEDGYSEIAIWPNN 315

[Close]

BLAST of CmaCh11G011260 vs. ExPASy Swiss-Prot
Match: P30364 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1)

HSP 1 Score: 472.6 bits (1215), Expect = 3.5e-132
Identity = 249/322 (77.33%), Postives = 274/322 (85.09%), Query Frame = 0


Query: 2   GWAIALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SL  +RRQPREE LR CL IGV+ALK QKPPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRQPREEGLRHCLQIGVEALKSQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 121
            HFNAG GSVLT +GTVEMEASIMDGK  +CGAVSGL+TV+N ISLARLVM+KTPHIYLA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGKTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYT-----QPSPQATVQET 181
           F GA+ FA++QGVET D S+FIT +N+ERLK A EAN VQ+DY+     QP+     +E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHFITAENVERLKLAIEANRVQVDYSQYNYPQPAQDDAEKEL 182

Query: 182 GIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSA 241
            + NGDSQIGTVGCVAVD+HGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN LCAVSA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKDLSLEAAAACAV-ESVPKGNVGLIAVSASGEVAMPFN 301
           TGKGEAII  TVARDVAALMEFK LSL+ AA   V E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEAIISATVARDVAALMEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEIAIWPS 317
           TTGMFRA ATEDGYSEIAIWP+
Sbjct: 303 TTGMFRASATEDGYSEIAIWPT 324

[Close]

BLAST of CmaCh11G011260 vs. ExPASy Swiss-Prot
Match: P50288 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1)

HSP 1 Score: 469.2 bits (1206), Expect = 3.8e-131
Identity = 245/322 (76.09%), Postives = 270/322 (83.85%), Query Frame = 0


Query: 2   GWAIALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SL  +RR+PREE LR CL IG++ALK Q PPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGIEALKAQNPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 121
            HFNAG GSVLT +GTVEMEASIMDG   +CGAVSGL TV+N ISLAR VM+KTPHI+LA
Sbjct: 63  QHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLNTVMNPISLARQVMDKTPHIFLA 122

Query: 122 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQID-----YTQPSPQATVQET 181
           F GA+   ++QGVET D S+FIT +N+ERLK A EAN VQ+D     YTQP      +E 
Sbjct: 123 FQGAQDLGKQQGVETVDSSHFITEENVERLKLAIEANRVQVDYSQYNYTQPVQDDAEKEL 182

Query: 182 GIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSA 241
            + NGDSQIGTVGCVAVD+HGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN LCAVSA
Sbjct: 183 PLANGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKDLSLEAAAACAV-ESVPKGNVGLIAVSASGEVAMPFN 301
           TGKGEAIIR TVARDVAALMEFK LSL+ AA C V E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEAIIRATVARDVAALMEFKGLSLKEAADCVVHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEIAIWPS 317
           TTGMFRACATEDGYSEIAIWP+
Sbjct: 303 TTGMFRACATEDGYSEIAIWPT 324

[Close]

BLAST of CmaCh11G011260 vs. ExPASy Swiss-Prot
Match: Q9ZSD6 (Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1)

HSP 1 Score: 468.4 bits (1204), Expect = 6.5e-131
Identity = 246/322 (76.40%), Postives = 272/322 (84.47%), Query Frame = 0


Query: 2   GWAIALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELENC 61
           GW+IALHGGAGDIP SL  +RR+PREE LR CL IGV+ALK QKPPLDVVELVVRELEN 
Sbjct: 3   GWSIALHGGAGDIPFSLPPERRKPREEGLRHCLQIGVEALKAQKPPLDVVELVVRELENI 62

Query: 62  PHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 121
            HFNAG GSVLT +GTVEMEASIMDG   +CGAVSGL+TV+N ISLARLVM+KTPHIYLA
Sbjct: 63  EHFNAGIGSVLTNSGTVEMEASIMDGNTMKCGAVSGLSTVLNPISLARLVMDKTPHIYLA 122

Query: 122 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQ-----PSPQATVQET 181
           F GA+ FA++QGVET D S+ IT +N+ERLK A EAN VQ+DY+Q     P      +E 
Sbjct: 123 FQGAQDFAKQQGVETVDSSHLITAENVERLKLAIEANRVQVDYSQYNYPEPVKDDAEKEL 182

Query: 182 GIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSA 241
            + NGDSQIGTVGCVAVD+HGNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN LCAVSA
Sbjct: 183 PLTNGDSQIGTVGCVAVDSHGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSA 242

Query: 242 TGKGEAIIRGTVARDVAALMEFKDLSLEAAAACAV-ESVPKGNVGLIAVSASGEVAMPFN 301
           TGKGE IIR TVARDVAALMEFK LSL+ AA   + E  PKG VGLIAVSA+GE+AMPFN
Sbjct: 243 TGKGEEIIRATVARDVAALMEFKGLSLKEAADFVIHERTPKGTVGLIAVSAAGEIAMPFN 302

Query: 302 TTGMFRACATEDGYSEIAIWPS 317
           TTGMFRACATEDGYSEIAIWP+
Sbjct: 303 TTGMFRACATEDGYSEIAIWPT 324

[Close]

BLAST of CmaCh11G011260 vs. ExPASy Swiss-Prot
Match: P30362 (Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2 SV=1)

HSP 1 Score: 432.6 bits (1111), Expect = 4.0e-120
Identity = 232/302 (76.82%), Postives = 255/302 (84.44%), Query Frame = 0


Query: 21  DRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELENCPHFNAGRGSVLTTNGTVEM 80
           +RR+PREE LR CL IGV+ALK +K PLDVVELVVRELEN  HFNAG GSVLT +GTVEM
Sbjct: 3   ERRKPREEGLRHCLQIGVEALKARKSPLDVVELVVRELENNEHFNAGIGSVLTNSGTVEM 62

Query: 81  EASIMDGKK-RCGAVSGLTTVVNAISLARLVMEKTPHIYLAFDGAEAFAREQGVETTDPS 140
           EASIMDGK  +CGAVSGL+TV+N ISLARLVMEKTPH+YLAF GA+ FA++QGVET D S
Sbjct: 63  EASIMDGKSMKCGAVSGLSTVLNPISLARLVMEKTPHMYLAFQGAQDFAKQQGVETVDSS 122

Query: 141 YFITPQNIERLKQAKEANSVQID-----YTQPSPQATVQETGIGNGDSQIGTVGCVAVDN 200
           +FIT +N+ERLK A EAN VQID     YTQP      +E  + NGDSQIGTVGCVAVD+
Sbjct: 123 HFITAENVERLKLAIEANRVQIDYSQYNYTQPVQDDAEKELPVANGDSQIGTVGCVAVDS 182

Query: 201 HGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGEAIIRGTVARDVAAL 260
            GNLA+ATSTGGLVNKMVGRIGDTPLIGAGTYAN LCAVSATGKGEAII+ TVARDVAAL
Sbjct: 183 QGNLASATSTGGLVNKMVGRIGDTPLIGAGTYANELCAVSATGKGEAIIQATVARDVAAL 242

Query: 261 MEFKDLSLEAAAACAV-ESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEIAI 316
           MEFK LSL+ AA   V E  PKG VGLIAVSA+GE+AMPFNTTGMFRACATEDG SEIAI
Sbjct: 243 MEFKGLSLKEAADYVVHERTPKGTVGLIAVSAAGEIAMPFNTTGMFRACATEDGNSEIAI 302

[Close]

BLAST of CmaCh11G011260 vs. TAIR 10
Match: AT5G08100.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 475.7 bits (1223), Expect = 2.9e-134
Identity = 249/319 (78.06%), Postives = 271/319 (84.95%), Query Frame = 0


Query: 1   MGWAIALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELEN 60
           +GWAIALHGGAGDIP+ L  +RR PRE  LR CL +G+ ALK  KPPLDV ELVVRELEN
Sbjct: 2   VGWAIALHGGAGDIPIDLPDERRIPRESALRHCLDLGISALKSGKPPLDVAELVVRELEN 61

Query: 61  CPHFNAGRGSVLTTNGTVEMEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYL 120
            P FNAG+GSVLT  GTVEMEASIMDGK KRCGAVSGLTTVVN ISLARLVMEKTPHIYL
Sbjct: 62  HPDFNAGKGSVLTAQGTVEMEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYL 121

Query: 121 AFDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETGIGN 180
           AFD AEAFAR  GVET D S+FITP+NI RLKQAKE N VQ+DYT PSP+          
Sbjct: 122 AFDAAEAFARAHGVETVDSSHFITPENIARLKQAKEFNRVQLDYTVPSPKVP-----DNC 181

Query: 181 GDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKG 240
           GDSQIGTVGCVAVD+ GNLA+ATSTGG VNKMVGRIGDTP+IGAGTYANHLCA+SATGKG
Sbjct: 182 GDSQIGTVGCVAVDSAGNLASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKG 241

Query: 241 EAIIRGTVARDVAALMEFKDLSLEAAAACAV-ESVPKGNVGLIAVSASGEVAMPFNTTGM 300
           E IIRGTVARDVAALME+K LSL  AAA  V +SVP+G+ GL+AVSA+GEV MPFNTTGM
Sbjct: 242 EDIIRGTVARDVAALMEYKGLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGM 301

Query: 301 FRACATEDGYSEIAIWPSN 318
           FRACA+EDGYSEIAIWP+N
Sbjct: 302 FRACASEDGYSEIAIWPNN 315

[Close]

BLAST of CmaCh11G011260 vs. TAIR 10
Match: AT5G08100.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 359.8 bits (922), Expect = 2.3e-99
Identity = 192/240 (80.00%), Postives = 208/240 (86.67%), Query Frame = 0


Query: 80  MEASIMDGK-KRCGAVSGLTTVVNAISLARLVMEKTPHIYLAFDGAEAFAREQGVETTDP 139
           MEASIMDGK KRCGAVSGLTTVVN ISLARLVMEKTPHIYLAFD AEAFAR  GVET D 
Sbjct: 1   MEASIMDGKTKRCGAVSGLTTVVNPISLARLVMEKTPHIYLAFDAAEAFARAHGVETVDS 60

Query: 140 SYFITPQNIERLKQAKEANSVQIDYTQPSPQATVQETGIGNGDSQIGTVGCVAVDNHGNL 199
           S+FITP+NI RLKQAKE N VQ+DYT PSP+          GDSQIGTVGCVAVD+ GNL
Sbjct: 61  SHFITPENIARLKQAKEFNRVQLDYTVPSPKVP-----DNCGDSQIGTVGCVAVDSAGNL 120

Query: 200 AAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCAVSATGKGEAIIRGTVARDVAALMEFK 259
           A+ATSTGG VNKMVGRIGDTP+IGAGTYANHLCA+SATGKGE IIRGTVARDVAALME+K
Sbjct: 121 ASATSTGGYVNKMVGRIGDTPVIGAGTYANHLCAISATGKGEDIIRGTVARDVAALMEYK 180

Query: 260 DLSLEAAAACAV-ESVPKGNVGLIAVSASGEVAMPFNTTGMFRACATEDGYSEIAIWPSN 318
            LSL  AAA  V +SVP+G+ GL+AVSA+GEV MPFNTTGMFRACA+EDGYSEIAIWP+N
Sbjct: 181 GLSLTEAAAYVVDQSVPRGSCGLVAVSANGEVTMPFNTTGMFRACASEDGYSEIAIWPNN 235

[Close]

BLAST of CmaCh11G011260 vs. TAIR 10
Match: AT3G16150.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 349.4 bits (895), Expect = 3.1e-96
Identity = 188/323 (58.20%), Postives = 233/323 (72.14%), Query Frame = 0


Query: 2   GWAIALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELENC 61
           GWAIA+HGGAG  P +L  +R++  ++ L RCL++G+ AL+     +DVVELV+RELE  
Sbjct: 3   GWAIAVHGGAGIDP-NLPAERQEEAKQLLTRCLNLGIIALRSNVSAIDVVELVIRELETD 62

Query: 62  PHFNAGRGSVLTTNGTVEMEASIMDG-KKRCGAVSGLTTVVNAISLARLVMEKTPHIYLA 121
           P FN+GRGS LT  GTVEMEASIMDG K+RCGAVSG+TTV N ISLARLVM+K+PH YLA
Sbjct: 63  PLFNSGRGSALTEKGTVEMEASIMDGTKRRCGAVSGITTVKNPISLARLVMDKSPHSYLA 122

Query: 122 FDGAEAFAREQGVETTDPSYFITPQNIERLKQAKEANSVQIDYTQP--------SPQATV 181
           F GAE FAR+QGVE  D  YF+T  N+  LK AKEANS+  DY  P        +  + +
Sbjct: 123 FSGAEDFARKQGVEIVDNEYFVTDDNVGMLKLAKEANSILFDYRIPPMGCAGAAATDSPI 182

Query: 182 QETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAGTYANHLCA 241
           Q  G+        TVGCV VD  G+ AA TSTGGL+NKM+GRIGD+PLIGAGTYA+  C 
Sbjct: 183 QMNGLPISIYAPETVGCVVVDGKGHCAAGTSTGGLMNKMMGRIGDSPLIGAGTYASEFCG 242

Query: 242 VSATGKGEAIIRGTVARDVAALMEFKDLSLEAAAACAVE-SVPKGNVGLIAVSASGEVAM 301
           VS TG+GEAIIR T+ARDV+A+ME+K L+L+ A    ++  + +G  GLIAVS  GEV  
Sbjct: 243 VSCTGEGEAIIRATLARDVSAVMEYKGLNLQEAVDYVIKHRLDEGFAGLIAVSNKGEVVC 302

Query: 302 PFNTTGMFRACATEDGYSEIAIW 315
            FN+ GMFR CATEDG+ E+AIW
Sbjct: 303 GFNSNGMFRGCATEDGFMEVAIW 324

[Close]

BLAST of CmaCh11G011260 vs. TAIR 10
Match: AT4G00590.1 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 95.9 bits (237), Expect = 6.3e-20
Identity = 87/335 (25.97%), Postives = 144/335 (42.99%), Query Frame = 0


Query: 5   IALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELENCPHF 64
           +A+H GAG      + + +  R    R CL       ++    +D V   ++ LE+ P  
Sbjct: 15  VAVHVGAG---YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 65  NAGRGSVLTTNGTVEMEASIMDGKK-RCGAVSGLTTVVNAISLARLVME----------K 124
           NAGRGS LT +G VE +AS+MDG     G V  +  V NAI +A L+++          +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDSGMFGGVGAVPGVRNAIKIAALLVKEQITGSTLLGR 134

Query: 125 TPHIYLAFDGAEAFAREQ-----GVETTDPSYFITPQNIERLKQAK----EANSVQIDYT 184
            P + L  +GA  + + +     G  T    + +T +   + ++ K    E  +  I   
Sbjct: 135 IPPMLLVGEGARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSA 194

Query: 185 QPSPQAT---------VQETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRI 244
           +  P+ T         V      + D  + TVG + VDN G++A  +S+GG+  K+ GR+
Sbjct: 195 EEHPRGTENNETCEENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSGGIAMKISGRV 254

Query: 245 GDTPLIGAGTYAN--------HLCAVSATGKGEAIIRGTVARDVAALMEFKDLSLEAAAA 293
           G     G+G +A+         L     +G GE ++RG  AR+    +        +AA 
Sbjct: 255 GLAATYGSGCWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAM 314

[Close]

BLAST of CmaCh11G011260 vs. TAIR 10
Match: AT4G00590.2 (N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein )

HSP 1 Score: 87.4 bits (215), Expect = 2.2e-17
Identity = 84/326 (25.77%), Postives = 141/326 (43.25%), Query Frame = 0


Query: 5   IALHGGAGDIPLSLSLDRRQPREETLRRCLHIGVQALKEQKPPLDVVELVVRELENCPHF 64
           +A+H GAG      + + +  R    R CL       ++    +D V   ++ LE+ P  
Sbjct: 15  VAVHVGAG---YHAAANEKALRSVMRRACLAASTILRQDSGECIDAVSAAIQVLEDDPST 74

Query: 65  NAGRGSVLTTNGTVEMEASIMDGKKRCGAVSGLTTVVNAIS--LARLVMEKTPHIYLAFD 124
           NAGRGS LT +G VE +AS+MDG    G   G+  V + +S  L++L++       L  +
Sbjct: 75  NAGRGSNLTEDGHVECDASLMDGDS--GMFGGVGAVPDQVSEMLSKLLL----CWLLVGE 134

Query: 125 GAEAFAREQ-----GVETTDPSYFITPQNIERLKQAK----EANSVQIDYTQPSPQAT-- 184
           GA  + + +     G  T    + +T +   + ++ K    E  +  I   +  P+ T  
Sbjct: 135 GARRWGKSKSVLIPGTVTEADQWLVTERARNQWRRFKAMLSEVGAKSILSAEEHPRGTEN 194

Query: 185 -------VQETGIGNGDSQIGTVGCVAVDNHGNLAAATSTGGLVNKMVGRIGDTPLIGAG 244
                  V      + D  + TVG + VDN G++A  +S+GG+  K+ GR+G     G+G
Sbjct: 195 NETCEENVSSCAAADEDKIMDTVGVICVDNEGHIACGSSSGGIAMKISGRVGLAATYGSG 254

Query: 245 TYAN--------HLCAVSATGKGEAIIRGTVARDVAALMEFKDLSLEAAAACAVESV--- 293
            +A+         L     +G GE ++RG  AR+    +        +AA   + SV   
Sbjct: 255 CWASSKGPFGAPFLVGCCVSGAGEYLMRGFAARECCTSLALSQAGPASAAMKVLRSVMHQ 314

The following BLAST results are available for this feature:

BLAST of CmaCh11G011260 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
P50287	4.1e-133	78.06	Isoaspartyl peptidase/L-asparaginase 1 OS=Arabidopsis thaliana OX=3702 GN=At5g08...	[more]
P30364	3.5e-132	77.33	Isoaspartyl peptidase/L-asparaginase OS=Lupinus angustifolius OX=3871 PE=2 SV=1	[more]
P50288	3.8e-131	76.09	Isoaspartyl peptidase/L-asparaginase OS=Lupinus albus OX=3870 PE=3 SV=1	[more]
Q9ZSD6	6.5e-131	76.40	Isoaspartyl peptidase/L-asparaginase OS=Lupinus luteus OX=3873 PE=1 SV=1	[more]
P30362	4.0e-120	76.82	Isoaspartyl peptidase/L-asparaginase (Fragment) OS=Lupinus arboreus OX=3872 PE=2...	[more]

BLAST of CmaCh11G011260 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G08100.1	2.9e-134	78.06	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT5G08100.2	2.3e-99	80.00	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT3G16150.1	3.1e-96	58.20	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.1	6.3e-20	25.97	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]
AT4G00590.2	2.2e-17	25.77	N-terminal nucleophile aminohydrolases (Ntn hydrolases) superfamily protein	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR000246	Peptidase T2, asparaginase 2	PFAM	PF01112	Asparaginase_2	coord: 3..314 e-value: 1.4E-111 score: 372.4
IPR000246	Peptidase T2, asparaginase 2	PANTHER	PTHR10188	L-ASPARAGINASE	coord: 1..314
None	No IPR available	GENE3D	3.60.20.30	(Glycosyl)asparaginase	coord: 186..317 e-value: 6.4E-44 score: 151.3
None	No IPR available	PIRSR	PIRSR600246-1	PIRSR600246-1	coord: 2..316 e-value: 4.7E-106 score: 352.4
None	No IPR available	PIRSR	PIRSR600246-2	PIRSR600246-2	coord: 2..316 e-value: 4.7E-106 score: 352.4
None	No IPR available	PIRSR	PIRSR600246-3	PIRSR600246-3	coord: 2..316 e-value: 4.7E-106 score: 352.4
None	No IPR available	PANTHER	PTHR10188:SF33	ISOASPARTYL PEPTIDASE/L-ASPARAGINASE	coord: 1..314
None	No IPR available	CDD	cd04701	Asparaginase_2	coord: 4..307 e-value: 2.05109E-128 score: 365.246
IPR029055	Nucleophile aminohydrolases, N-terminal	SUPERFAMILY	56235	N-terminal nucleophile aminohydrolases (Ntn hydrolases)	coord: 25..317

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh11G011260.1	CmaCh11G011260.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016540	protein autoprocessing
cellular_component	GO:0005737	cytoplasm
molecular_function	GO:0004067	asparaginase activity
molecular_function	GO:0008798	beta-aspartyl-peptidase activity
molecular_function	GO:0016787	hydrolase activity