CmaCh11G009860 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh11G009860
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	mitochondrial import inner membrane translocase subunit TIM23-2-like
Location	Cma_Chr11: 5246207 .. 5246758 (-)
RNA-Seq Expression	CmaCh11G009860
Synteny	CmaCh11G009860

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGAAGATTCCCCCCAATCAGCCGGTTATCGGAAGACCCGTTTCTACCACCCCTACCAAGACCTTCACGTCCCGATCACCAAGCTTTACGAGCTCCCCACCGCCCCAGAACATCTTTTCTTCGAAGAGGCGGCTAGGCCCCACCGTTCTTGGGGCGAAAATCTCCAGTACTACACCGGAATCGGCTACTTGTCCGGTGCCCTTTTTGGCGGCGCCAGGGGATCTCTCCAGGGAATTAGGGCGGCGGAGCCTGGCGACACCATGAAGCTTCGTCTTAATCGAGTTTTGAACTCTGGGGGTCAGGTCGGCCGCAGGGCTGGGAACTCTCTTGGGATTCTTGGCTTGATTTTTGCTGGTTTGGAAAGTGGGGTTACTCATTTGAGAGGCTCTGATGATGTTTTGAATAGTATTGTTGCTGGATTGGGGACTGGTGCAATTTATAAGGCGGCTTCAGGGCCGAGGTCGGCCGCCATCGCTGGTGCTATTGGAGGGATTGCTGCTGCAGCTGCGGTGGCGGGCAAGCAGGCAGCGAAGAGATATGTGCCAATATAG

mRNA sequence

ATGGAAGATTCCCCCCAATCAGCCGGTTATCGGAAGACCCGTTTCTACCACCCCTACCAAGACCTTCACGTCCCGATCACCAAGCTTTACGAGCTCCCCACCGCCCCAGAACATCTTTTCTTCGAAGAGGCGGCTAGGCCCCACCGTTCTTGGGGCGAAAATCTCCAGTACTACACCGGAATCGGCTACTTGTCCGGTGCCCTTTTTGGCGGCGCCAGGGGATCTCTCCAGGGAATTAGGGCGGCGGAGCCTGGCGACACCATGAAGCTTCGTCTTAATCGAGTTTTGAACTCTGGGGGTCAGGTCGGCCGCAGGGCTGGGAACTCTCTTGGGATTCTTGGCTTGATTTTTGCTGGTTTGGAAAGTGGGGTTACTCATTTGAGAGGCTCTGATGATGTTTTGAATAGTATTGTTGCTGGATTGGGGACTGGTGCAATTTATAAGGCGGCTTCAGGGCCGAGGTCGGCCGCCATCGCTGGTGCTATTGGAGGGATTGCTGCTGCAGCTGCGGTGGCGGGCAAGCAGGCAGCGAAGAGATATGTGCCAATATAG

Coding sequence (CDS)

ATGGAAGATTCCCCCCAATCAGCCGGTTATCGGAAGACCCGTTTCTACCACCCCTACCAAGACCTTCACGTCCCGATCACCAAGCTTTACGAGCTCCCCACCGCCCCAGAACATCTTTTCTTCGAAGAGGCGGCTAGGCCCCACCGTTCTTGGGGCGAAAATCTCCAGTACTACACCGGAATCGGCTACTTGTCCGGTGCCCTTTTTGGCGGCGCCAGGGGATCTCTCCAGGGAATTAGGGCGGCGGAGCCTGGCGACACCATGAAGCTTCGTCTTAATCGAGTTTTGAACTCTGGGGGTCAGGTCGGCCGCAGGGCTGGGAACTCTCTTGGGATTCTTGGCTTGATTTTTGCTGGTTTGGAAAGTGGGGTTACTCATTTGAGAGGCTCTGATGATGTTTTGAATAGTATTGTTGCTGGATTGGGGACTGGTGCAATTTATAAGGCGGCTTCAGGGCCGAGGTCGGCCGCCATCGCTGGTGCTATTGGAGGGATTGCTGCTGCAGCTGCGGTGGCGGGCAAGCAGGCAGCGAAGAGATATGTGCCAATATAG

Protein sequence

MEDSPQSAGYRKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYVPI

Homology

[Close]

BLAST of CmaCh11G009860 vs. ExPASy Swiss-Prot
Match: Q9S837 (Mitochondrial import inner membrane translocase subunit TIM23-3 OS=Arabidopsis thaliana OX=3702 GN=TIM23-3 PE=2 SV=1)

HSP 1 Score: 201.8 bits (512), Expect = 6.5e-51
Identity = 99/174 (56.90%), Postives = 137/174 (78.74%), Query Frame = 0


Query: 11  RKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALFG 70
           +K R Y+PYQ +++P  KLYELPT+PE LF EEA +   +WGENL ++TG GY +G++ G
Sbjct: 15  QKYRQYNPYQQVNLPYRKLYELPTSPEFLFEEEATKKRLTWGENLTFFTGWGYCTGSVLG 74

Query: 71  GARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTHLR-G 130
             +G++ G+RAAE G+++K+R NR+LNSGG V RR GN LG +GL+FA +ESGVT++R G
Sbjct: 75  AFKGTIAGMRAAERGESLKIRTNRILNSGGLVARRGGNCLGSVGLMFAAMESGVTYMRDG 134

Query: 131 SDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYVPI 184
            D  L +++AGL TG +Y+AASGPRSA +AGA+GG+AA AAVAG++  KR+VPI
Sbjct: 135 DDGSLTTVIAGLATGVLYRAASGPRSAVVAGAVGGVAALAAVAGRRIVKRFVPI 188

[Close]

BLAST of CmaCh11G009860 vs. ExPASy Swiss-Prot
Match: Q38820 (Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Arabidopsis thaliana OX=3702 GN=TIM23-2 PE=1 SV=1)

HSP 1 Score: 201.4 bits (511), Expect = 8.5e-51
Identity = 103/174 (59.20%), Postives = 130/174 (74.71%), Query Frame = 0


Query: 13  TRFYHPYQDLHVPITK---LYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALF 72
           TR Y+PYQ+  VPI K   LY+LPT+PE LF EEA R  RSWGENL +YTG  YL G++ 
Sbjct: 15  TRLYNPYQNYEVPINKSQYLYKLPTSPEFLFTEEALRQRRSWGENLTFYTGTAYLGGSVA 74

Query: 73  GGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTHLRG 132
           G + G + G+++ E GDT KL++NR+LNS GQ GR  GN +GI+GL++AG+ESG+     
Sbjct: 75  GASVGVITGVKSFESGDTTKLKINRILNSSGQTGRTWGNRIGIIGLVYAGIESGIVAATD 134

Query: 133 SDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYVPI 184
            DDV  S+VAGLGTGA+ +AA G RSAA+AGA+GG+AA A VAGKQ  KRYVPI
Sbjct: 135 RDDVWTSVVAGLGTGAVCRAARGVRSAAVAGALGGLAAGAVVAGKQIVKRYVPI 188

[Close]

BLAST of CmaCh11G009860 vs. ExPASy Swiss-Prot
Match: Q9LNQ1 (Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Arabidopsis thaliana OX=3702 GN=TIM23-1 PE=2 SV=1)

HSP 1 Score: 200.7 bits (509), Expect = 1.5e-50
Identity = 104/173 (60.12%), Postives = 129/173 (74.57%), Query Frame = 0


Query: 13  TRFYHPYQDLHVPITK--LYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALFG 72
           TR YHPYQ+  VPI    LY+LPT+PE LF EE+ +  RSWGENL +YTG GYL+G++ G
Sbjct: 15  TRLYHPYQNYQVPIKSQYLYKLPTSPEFLFTEESLKQRRSWGENLTFYTGTGYLAGSVAG 74

Query: 73  GARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTHLRGS 132
            + G   GI++ E GDT KL++NR+LNS GQ GR  GN +GI+GLI+AG+ESGV  +   
Sbjct: 75  ASAGIFSGIKSFENGDTTKLKINRILNSSGQAGRTWGNRVGIVGLIYAGIESGVVAVTDK 134

Query: 133 DDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYVPI 184
           DDV  S+VAGLGTGA+++AA G RSAA+AGA GGIAA A VAGKQ  KRY  I
Sbjct: 135 DDVWTSVVAGLGTGAVFRAARGVRSAAVAGAFGGIAAGAVVAGKQVFKRYAHI 187

[Close]

BLAST of CmaCh11G009860 vs. ExPASy Swiss-Prot
Match: Q9USM7 (Mitochondrial import inner membrane translocase subunit tim23 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=tim23 PE=3 SV=1)

HSP 1 Score: 84.0 bits (206), Expect = 2.0e-15
Identity = 57/160 (35.62%), Postives = 84/160 (52.50%), Query Frame = 0


Query: 17  HPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALFGGARGSL 76
           HP  DL  P+  L     A   L  +  A P R W ++L Y TG  YLSG   GG  G  
Sbjct: 45  HPLADLDKPLDYLLIEEDALSTLPGDSMAIPSRGWQDDLCYGTGTSYLSGLAIGGLWGLN 104

Query: 77  QGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTHLRGSDDVLNS 136
           +G++  +   + +LRLN +LN   + G   GNSLG+L L++ G+ S + + R      NS
Sbjct: 105 EGMKKTKDITSTRLRLNGILNGVTRRGPFVGNSLGVLALVYNGINSLIGYKRQKHGWENS 164

Query: 137 IVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQA 177
           + AG  TGA+YK+  G R+ AI+ ++   AA      K++
Sbjct: 165 VAAGALTGALYKSTRGLRAMAISSSLVATAAGIWTLAKRS 204

[Close]

BLAST of CmaCh11G009860 vs. ExPASy Swiss-Prot
Match: P32897 (Mitochondrial import inner membrane translocase subunit TIM23 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=TIM23 PE=1 SV=1)

HSP 1 Score: 73.2 bits (178), Expect = 3.5e-12
Identity = 50/125 (40.00%), Postives = 69/125 (55.20%), Query Frame = 0


Query: 47  PHRSWGENLQYYTGIGYLSGALFGGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRA 106
           P R W ++L Y TG  YL G   GG  G +QG++   P    KL+LN VLN   + G   
Sbjct: 89  PSRGWTDDLCYGTGAVYLLGLGIGGFSGMMQGLQNIPPNSPGKLQLNTVLNHITKRGPFL 148

Query: 107 GNSLGILGLIFAGLESGVTHLRGSDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIA 166
           GN+ GIL L +  + S +  LRG  D   SI AG  TGA++K++ G +    + A+  +A
Sbjct: 149 GNNAGILALSYNIINSTIDALRGKHDTAGSIGAGALTGALFKSSKGLKPMGYSSAM--VA 208

Query: 167 AAAAV 172
           AA AV
Sbjct: 209 AACAV 211

[Close]

BLAST of CmaCh11G009860 vs. TAIR 10
Match: AT3G04800.1 (translocase inner membrane subunit 23-3 )

HSP 1 Score: 201.8 bits (512), Expect = 4.6e-52
Identity = 99/174 (56.90%), Postives = 137/174 (78.74%), Query Frame = 0


Query: 11  RKTRFYHPYQDLHVPITKLYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALFG 70
           +K R Y+PYQ +++P  KLYELPT+PE LF EEA +   +WGENL ++TG GY +G++ G
Sbjct: 15  QKYRQYNPYQQVNLPYRKLYELPTSPEFLFEEEATKKRLTWGENLTFFTGWGYCTGSVLG 74

Query: 71  GARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTHLR-G 130
             +G++ G+RAAE G+++K+R NR+LNSGG V RR GN LG +GL+FA +ESGVT++R G
Sbjct: 75  AFKGTIAGMRAAERGESLKIRTNRILNSGGLVARRGGNCLGSVGLMFAAMESGVTYMRDG 134

Query: 131 SDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYVPI 184
            D  L +++AGL TG +Y+AASGPRSA +AGA+GG+AA AAVAG++  KR+VPI
Sbjct: 135 DDGSLTTVIAGLATGVLYRAASGPRSAVVAGAVGGVAALAAVAGRRIVKRFVPI 188

[Close]

BLAST of CmaCh11G009860 vs. TAIR 10
Match: AT1G72750.1 (translocase inner membrane subunit 23-2 )

HSP 1 Score: 201.4 bits (511), Expect = 6.1e-52
Identity = 103/174 (59.20%), Postives = 130/174 (74.71%), Query Frame = 0


Query: 13  TRFYHPYQDLHVPITK---LYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALF 72
           TR Y+PYQ+  VPI K   LY+LPT+PE LF EEA R  RSWGENL +YTG  YL G++ 
Sbjct: 15  TRLYNPYQNYEVPINKSQYLYKLPTSPEFLFTEEALRQRRSWGENLTFYTGTAYLGGSVA 74

Query: 73  GGARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTHLRG 132
           G + G + G+++ E GDT KL++NR+LNS GQ GR  GN +GI+GL++AG+ESG+     
Sbjct: 75  GASVGVITGVKSFESGDTTKLKINRILNSSGQTGRTWGNRIGIIGLVYAGIESGIVAATD 134

Query: 133 SDDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYVPI 184
            DDV  S+VAGLGTGA+ +AA G RSAA+AGA+GG+AA A VAGKQ  KRYVPI
Sbjct: 135 RDDVWTSVVAGLGTGAVCRAARGVRSAAVAGALGGLAAGAVVAGKQIVKRYVPI 188

[Close]

BLAST of CmaCh11G009860 vs. TAIR 10
Match: AT1G17530.1 (translocase of inner mitochondrial membrane 23 )

HSP 1 Score: 200.7 bits (509), Expect = 1.0e-51
Identity = 104/173 (60.12%), Postives = 129/173 (74.57%), Query Frame = 0


Query: 13  TRFYHPYQDLHVPITK--LYELPTAPEHLFFEEAARPHRSWGENLQYYTGIGYLSGALFG 72
           TR YHPYQ+  VPI    LY+LPT+PE LF EE+ +  RSWGENL +YTG GYL+G++ G
Sbjct: 15  TRLYHPYQNYQVPIKSQYLYKLPTSPEFLFTEESLKQRRSWGENLTFYTGTGYLAGSVAG 74

Query: 73  GARGSLQGIRAAEPGDTMKLRLNRVLNSGGQVGRRAGNSLGILGLIFAGLESGVTHLRGS 132
            + G   GI++ E GDT KL++NR+LNS GQ GR  GN +GI+GLI+AG+ESGV  +   
Sbjct: 75  ASAGIFSGIKSFENGDTTKLKINRILNSSGQAGRTWGNRVGIVGLIYAGIESGVVAVTDK 134

Query: 133 DDVLNSIVAGLGTGAIYKAASGPRSAAIAGAIGGIAAAAAVAGKQAAKRYVPI 184
           DDV  S+VAGLGTGA+++AA G RSAA+AGA GGIAA A VAGKQ  KRY  I
Sbjct: 135 DDVWTSVVAGLGTGAVFRAARGVRSAAVAGAFGGIAAGAVVAGKQVFKRYAHI 187

The following BLAST results are available for this feature:

BLAST of CmaCh11G009860 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9S837	6.5e-51	56.90	Mitochondrial import inner membrane translocase subunit TIM23-3 OS=Arabidopsis t...	[more]
Q38820	8.5e-51	59.20	Mitochondrial import inner membrane translocase subunit TIM23-2 OS=Arabidopsis t...	[more]
Q9LNQ1	1.5e-50	60.12	Mitochondrial import inner membrane translocase subunit TIM23-1 OS=Arabidopsis t...	[more]
Q9USM7	2.0e-15	35.63	Mitochondrial import inner membrane translocase subunit tim23 OS=Schizosaccharom...	[more]
P32897	3.5e-12	40.00	Mitochondrial import inner membrane translocase subunit TIM23 OS=Saccharomyces c...	[more]

BLAST of CmaCh11G009860 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G04800.1	4.6e-52	56.90	translocase inner membrane subunit 23-3	[more]
AT1G72750.1	6.1e-52	59.20	translocase inner membrane subunit 23-2	[more]
AT1G17530.1	1.0e-51	60.12	translocase of inner mitochondrial membrane 23	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
None	No IPR available	PFAM	PF02466	Tim17	coord: 57..165 e-value: 1.2E-23 score: 83.7
None	No IPR available	PANTHER	PTHR15371	TIM23	coord: 1..183
None	No IPR available	PANTHER	PTHR15371:SF24	MITOCHONDRIAL IMPORT INNER MEMBRANE TRANSLOCASE SUBUNIT TIM23-3	coord: 1..183

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh11G009860.1	CmaCh11G009860.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0030150	protein import into mitochondrial matrix
cellular_component	GO:0031305	integral component of mitochondrial inner membrane
cellular_component	GO:0005744	TIM23 mitochondrial import inner membrane translocase complex
molecular_function	GO:0008320	protein transmembrane transporter activity