CmaCh11G008800 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh11G008800
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionolee1-like protein
LocationCma_Chr11: 4457313 .. 4457923 (+)
RNA-Seq ExpressionCmaCh11G008800
SyntenyCmaCh11G008800
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
AACAAGCACACCACAAGAGAAAAAAATGGCAAAATCCTTTGCTATCATTCTTTTTGCCCTTTGCTTCCTCTCGACCCTCGACGTCTCCGTCTCGAAAAAAGATCACTTCGTCGTCGAGGGCAAAGTTTATTGTGACACGTGCCGCATCCAATTCTTCACCCGTGTCAGTAAATGGATGCATGGTAACACAACTCCTTCGTTCATTCCTTTTGTATCATATGTTCAAACTCTTGTAATGTCATTTCTTTATAATGTATCGTGTATCAGGAGCTAAGGTGAAGTTGGTATGCCGTGAGGAGGAGGGAGGAAGCGAGACGTTAGCCGTCGAGGCGGAGACCGGCAAGAACGGAGAATACACGATCAAGGTGGACGGTGACCATGAGGAGGAGGTTTGTGAAGTGAATTTGGTGAAGAGTACTGATGATGACTGTGCTGAGATTCCCACGGATGGATTTGGGCATAGGGCGAGAGTGAGCATCACGGCCAACGATGGTATCACCAACCCTGTGCGTCAACCTAACCCACTTGGGTTCATGAAGAAGGAGACACTTCCCCAATGCAAAGAGGTTCTTCGTGAGCTTGGGTTCGATGAGGCTGGTCTTCCCGTTTGA

mRNA sequence

AACAAGCACACCACAAGAGAAAAAAATGGCAAAATCCTTTGCTATCATTCTTTTTGCCCTTTGCTTCCTCTCGACCCTCGACGTCTCCGTCTCGAAAAAAGATCACTTCGTCGTCGAGGGCAAAGTTTATTGTGACACGTGCCGCATCCAATTCTTCACCCGTGTCAGTAAATGGATGCATGGAGCTAAGGTGAAGTTGGTATGCCGTGAGGAGGAGGGAGGAAGCGAGACGTTAGCCGTCGAGGCGGAGACCGGCAAGAACGGAGAATACACGATCAAGGTGGACGGTGACCATGAGGAGGAGGTTTGTGAAGTGAATTTGGTGAAGAGTACTGATGATGACTGTGCTGAGATTCCCACGGATGGATTTGGGCATAGGGCGAGAGTGAGCATCACGGCCAACGATGGTATCACCAACCCTGTGCGTCAACCTAACCCACTTGGGTTCATGAAGAAGGAGACACTTCCCCAATGCAAAGAGGTTCTTCGTGAGCTTGGGTTCGATGAGGCTGGTCTTCCCGTTTGA

Coding sequence (CDS)

ATGGCAAAATCCTTTGCTATCATTCTTTTTGCCCTTTGCTTCCTCTCGACCCTCGACGTCTCCGTCTCGAAAAAAGATCACTTCGTCGTCGAGGGCAAAGTTTATTGTGACACGTGCCGCATCCAATTCTTCACCCGTGTCAGTAAATGGATGCATGGAGCTAAGGTGAAGTTGGTATGCCGTGAGGAGGAGGGAGGAAGCGAGACGTTAGCCGTCGAGGCGGAGACCGGCAAGAACGGAGAATACACGATCAAGGTGGACGGTGACCATGAGGAGGAGGTTTGTGAAGTGAATTTGGTGAAGAGTACTGATGATGACTGTGCTGAGATTCCCACGGATGGATTTGGGCATAGGGCGAGAGTGAGCATCACGGCCAACGATGGTATCACCAACCCTGTGCGTCAACCTAACCCACTTGGGTTCATGAAGAAGGAGACACTTCCCCAATGCAAAGAGGTTCTTCGTGAGCTTGGGTTCGATGAGGCTGGTCTTCCCGTTTGA

Protein sequence

MAKSFAIILFALCFLSTLDVSVSKKDHFVVEGKVYCDTCRIQFFTRVSKWMHGAKVKLVCREEEGGSETLAVEAETGKNGEYTIKVDGDHEEEVCEVNLVKSTDDDCAEIPTDGFGHRARVSITANDGITNPVRQPNPLGFMKKETLPQCKEVLRELGFDEAGLPV
Homology
BLAST of CmaCh11G008800 vs. ExPASy Swiss-Prot
Match: O49813 (Olee1-like protein OS=Betula pendula OX=3505 PE=2 SV=1)

HSP 1 Score: 177.2 bits (448), Expect = 1.6e-43
Identity = 90/161 (55.90%), Postives = 114/161 (70.81%), Query Frame = 0

Query: 1   MAKSFAIILFALCFLSTLDVSVSKKDHFVVEGKVYCDTCRIQFFTRVSKWMHGAKVKLVC 60
           MAKS  I   ALCFLS L  + S +  F VEGKVYCD CR QF T++S +M GAKV L C
Sbjct: 1   MAKSIIIQAPALCFLSLLGFAYS-ESRFFVEGKVYCDNCRTQFVTKLSTYMKGAKVSLEC 60

Query: 61  REEEGGSETLAVEAETGKNGEYTIKVDGDHEEEVCEVNLVKSTDDDCAEIPTDGFGHR-A 120
           R  EGG+   + ++ET K+G Y I VDGDHEEE+CE+ L KS+D DC+E+  D F  + A
Sbjct: 61  RNREGGTLIYSSDSETDKSGTYRIPVDGDHEEEICEIALKKSSDPDCSEVSKDPFLKKSA 120

Query: 121 RVSITANDGITNPVRQPNPLGFMKKETLPQCKEVLRELGFD 161
           R+S+T N+GI+ PVR  NPLGFMKK+ LP+C + LRELG +
Sbjct: 121 RISLTKNNGISTPVRLANPLGFMKKKPLPECAKALRELGMN 160

BLAST of CmaCh11G008800 vs. ExPASy Swiss-Prot
Match: Q8LGR0 (Pollen allergen Che a 1 OS=Chenopodium album OX=3559 PE=1 SV=1)

HSP 1 Score: 156.0 bits (393), Expect = 3.7e-37
Identity = 79/160 (49.38%), Postives = 110/160 (68.75%), Query Frame = 0

Query: 1   MAKSFAIILF--ALCFLSTLDVSVSKKDHFVVEGKVYCDTCRIQFFTRVSKWMHGAKVKL 60
           MAK  A+ L   ALC LS   V+ + ++HF V+G VYCDTCRIQF TR+S  M GA VKL
Sbjct: 1   MAKCQAVFLLVGALCVLSLAGVANAAENHFKVQGMVYCDTCRIQFMTRISTIMEGATVKL 60

Query: 61  VCREEEGGSETLAVEAETGKNGEYTIKVDGDHEEEVCEVNLVKSTDDDCAEIPTDGFGHR 120
            CR    G++T   EA T K G+Y+I V+GD E+++CE+ LVKS + +C+E+  D +  +
Sbjct: 61  ECRNITAGTQTFKAEAVTDKVGQYSIPVNGDFEDDICEIELVKSPNSECSEVSHDVYAKQ 120

Query: 121 -ARVSITANDGITNPVRQPNPLGFMKKETLPQCKEVLREL 158
            A+VS+T+N+G  + +R  N LGFM+KE L +C EVL+EL
Sbjct: 121 SAKVSLTSNNGEASDIRSANALGFMRKEPLKECPEVLKEL 160

BLAST of CmaCh11G008800 vs. ExPASy Swiss-Prot
Match: Q29W25 (Pollen allergen Cro s 1 OS=Crocus sativus OX=82528 PE=1 SV=1)

HSP 1 Score: 152.9 bits (385), Expect = 3.2e-36
Identity = 78/160 (48.75%), Postives = 108/160 (67.50%), Query Frame = 0

Query: 1   MAKSFAIILF--ALCFLSTLDVSVSKKDHFVVEGKVYCDTCRIQFFTRVSKWMHGAKVKL 60
           M K  A+ L   ALC LS   V+ + ++HF V+G VYCDTCRIQF TRVS  M GA VKL
Sbjct: 1   MGKCQAVFLLVGALCVLSLAGVANAAENHFKVQGMVYCDTCRIQFMTRVSTIMEGATVKL 60

Query: 61  VCREEEGGSETLAVEAETGKNGEYTIKVDGDHEEEVCEVNLVKSTDDDCAEIPTDGFGHR 120
            CR    G++T   EA T K G+Y+I V GD ++++CE+ LVKS + +C+E+  D +  +
Sbjct: 61  ECRNITAGTQTFKAEAVTDKVGQYSIPVHGDFQDDICEIELVKSPNSECSEVSHDVYAKQ 120

Query: 121 -ARVSITANDGITNPVRQPNPLGFMKKETLPQCKEVLREL 158
            A+VS+T+N+G  + +R  N LGFM+KE L +C EVL+EL
Sbjct: 121 SAKVSLTSNNGEASDIRSANALGFMRKEPLKECPEVLKEL 160

BLAST of CmaCh11G008800 vs. ExPASy Swiss-Prot
Match: E2D0Z0 (Pollen allergen Sal k 5.0101 (Fragment) OS=Kali turgidum OX=151250 PE=1 SV=1)

HSP 1 Score: 149.1 bits (375), Expect = 4.6e-35
Identity = 72/129 (55.81%), Postives = 92/129 (71.32%), Query Frame = 0

Query: 30  VEGKVYCDTCRIQFFTRVSKWMHGAKVKLVCREEEGGSETLAVEAETGKNGEYTIKVDGD 89
           V+G VYCDTCRIQF TRVS  M GA V L CR    G+ET   EA T K G YTIKVDGD
Sbjct: 11  VKGMVYCDTCRIQFITRVSTMMEGATVSLQCRNLTAGTETFKAEAVTDKLGMYTIKVDGD 70

Query: 90  HEEEVCEVNLVKSTDDDCAEIPTDGFGHR-ARVSITANDGITNPVRQPNPLGFMKKETLP 149
           HE+++C++ LVKS   +C+EIP D +  + A+V++T+N+G  + VR  N LGF+KK  LP
Sbjct: 71  HEDDICQIVLVKSPGQECSEIPNDVYSEQAAKVTLTSNNGEASDVRNANALGFLKKAPLP 130

Query: 150 QCKEVLREL 158
           +C EVL+EL
Sbjct: 131 ECPEVLKEL 139

BLAST of CmaCh11G008800 vs. ExPASy Swiss-Prot
Match: P13447 (Anther-specific protein LAT52 OS=Solanum lycopersicum OX=4081 GN=LAT52 PE=2 SV=1)

HSP 1 Score: 142.1 bits (357), Expect = 5.6e-33
Identity = 76/158 (48.10%), Postives = 105/158 (66.46%), Query Frame = 0

Query: 1   MAKSFAIILFALCFLSTLDVSVSKKDHFVVEGKVYCDTCRIQFFTRVSKWMHGAKVKLVC 60
           MAK+  ++L ALC L+  + +  + + F VEGKVYCDTCR+QF T++S+ + GA VKL C
Sbjct: 1   MAKAI-VLLSALCILALANFAHCRPEVFDVEGKVYCDTCRVQFETKLSENLEGATVKLQC 60

Query: 61  REEEGGSETLAVEAETGKNGEYTIKVDGDHEEEVCEVNLVKSTDDDCAEIPTDGFGHRAR 120
           R     +ET +VE  T K+G+Y + V+GDHE ++CEV +VKS  +DC E    G+  +AR
Sbjct: 61  RNISTEAETFSVEGVTDKDGKYKLTVNGDHENDICEVTVVKSPREDCKE-SVSGY-EKAR 120

Query: 121 VSITANDGITNPVRQPNPLGFMKKETLPQCKEVLRELG 159
           +  + N GI N VR  NPL FMK E++  CKE L ELG
Sbjct: 121 IECSDNVGIHNAVRFANPLFFMKAESVQGCKEALDELG 155

BLAST of CmaCh11G008800 vs. TAIR 10
Match: AT1G29140.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 149.4 bits (376), Expect = 2.5e-36
Identity = 79/164 (48.17%), Postives = 108/164 (65.85%), Query Frame = 0

Query: 1   MAKSFAIIL-FALCFLSTLDVSVSKK---DHFVVEGKVYCDTCRIQFFTRVSKWMHGAKV 60
           MAK F + L  ALCF + +  + +     D F ++G VYCDTCR+QF TR+SK++ GAKV
Sbjct: 1   MAKFFIVFLASALCFTTLVHFTAADADDFDKFHIKGSVYCDTCRVQFITRISKFLEGAKV 60

Query: 61  KLVCREEEGGSETLAVEAETGKNGEYTIKVDGDHEEEVCEVNLVKSTDDDCAEIPTDGF- 120
           KL C+  E  + TL  EA T   G Y ++V GDHEEEVCE+ L++S D +C ++    F 
Sbjct: 61  KLECKGRENQTVTLTKEAVTDNAGNYQMEVMGDHEEEVCEIVLLQSPDPECGDVNNQEFL 120

Query: 121 GHRARVSITANDGI-TNPVRQPNPLGFMKKETLPQCKEVLRELG 159
            + AR+S+TANDGI +N  R  NPLGFM+K  L +C +V +ELG
Sbjct: 121 RNAARISLTANDGIVSNETRTINPLGFMRKTPLAECPQVFKELG 164

BLAST of CmaCh11G008800 vs. TAIR 10
Match: AT5G45880.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 142.5 bits (358), Expect = 3.0e-34
Identity = 69/135 (51.11%), Postives = 93/135 (68.89%), Query Frame = 0

Query: 26  DHFVVEGKVYCDTCRIQFFTRVSKWMHGAKVKLVCREEEGGSETLAVEAETGKNGEYTIK 85
           D F ++G VYCDTCR+QF TR+SK++ GAKVKL CR    G+ TL  EA T K G Y ++
Sbjct: 33  DRFQIQGSVYCDTCRVQFVTRLSKFLEGAKVKLECRSRTNGTITLTKEAVTDKTGSYKME 92

Query: 86  VDGDHEEEVCEVNLVKSTDDDCAEIPTDGF-GHRARVSITANDGI-TNPVRQPNPLGFMK 145
           V GDHEEEVCE+ LV+S D  C+++ T+ +  + A++S+TANDGI ++  R  NPLGFM 
Sbjct: 93  VTGDHEEEVCELVLVQSPDSGCSDVSTEAYLRNAAKISLTANDGIVSHETRIVNPLGFMV 152

Query: 146 KETLPQCKEVLRELG 159
           +  L  C    +ELG
Sbjct: 153 QTPLADCPAAFKELG 167

BLAST of CmaCh11G008800 vs. TAIR 10
Match: AT4G18596.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 141.4 bits (355), Expect = 6.7e-34
Identity = 72/159 (45.28%), Postives = 102/159 (64.15%), Query Frame = 0

Query: 5   FAIILFALCFLSTLDVSVSKK---DHFVVEGKVYCDTCRIQFFTRVSKWMHGAKVKLVCR 64
           F   + A+C  S   V+++     D F ++G VYCDTCR+QF TR+SK++ GAKVKL CR
Sbjct: 7   FFFFVSAVCLSSLAGVAIADADDFDRFQIQGSVYCDTCRVQFVTRLSKFLEGAKVKLECR 66

Query: 65  EEEGGSETLAVEAETGKNGEYTIKVDGDHEEEVCEVNLVKSTDDDCAEIPTDGF-GHRAR 124
               G+ TL  EA T K G Y ++V GDHEEEVCE+ LV+S D  C+++  + +  + A+
Sbjct: 67  SRTNGTVTLTKEAVTDKTGSYRMEVTGDHEEEVCELVLVESPDSGCSDVSKEAYLRNAAK 126

Query: 125 VSITANDGI-TNPVRQPNPLGFMKKETLPQCKEVLRELG 159
           +S+TANDGI ++  R  NPLGFM +    +C    +ELG
Sbjct: 127 ISLTANDGIVSHETRIVNPLGFMVQTPSAECPAAFKELG 165

BLAST of CmaCh11G008800 vs. TAIR 10
Match: AT4G08685.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 108.2 bits (269), Expect = 6.3e-24
Identity = 59/154 (38.31%), Postives = 91/154 (59.09%), Query Frame = 0

Query: 4   SFAIILFALCFLSTLDVSV-SKKDHFVVEGKVYCDTCRIQFFTRVSKWMHGAKVKLVCRE 63
           S A++L ALCFL  L ++    K+ FVV G+VYCDTC   F T  S ++ GA V+L C++
Sbjct: 2   SKAVLLVALCFLPALAIAARPNKNPFVVRGRVYCDTCLAGFETPASTYISGAVVRLECKD 61

Query: 64  EEGGSETLAVEAETGKNGEYTIKVDGDHEEEVCEVNLVKSTDDDCAEIPTDGFGH-RARV 123
                 T + EA T   G Y I V+ DH+E+ C+  LV+S+   C+ +     GH RARV
Sbjct: 62  RRTMELTYSHEARTDSTGSYKILVNEDHDEQFCDAMLVRSSQLRCSNVSP---GHDRARV 121

Query: 124 SITANDGITNPVRQPNPLGFMKKETLPQCKEVLR 156
           ++T  +GI +  R  N +GF++   +P C ++++
Sbjct: 122 TLTRFNGIASDDRFANNMGFLRDAAMPGCADIMK 152

BLAST of CmaCh11G008800 vs. TAIR 10
Match: AT5G10130.1 (Pollen Ole e 1 allergen and extensin family protein )

HSP 1 Score: 88.6 bits (218), Expect = 5.2e-18
Identity = 51/150 (34.00%), Postives = 78/150 (52.00%), Query Frame = 0

Query: 1   MAKSFAIILFALCFLSTLDVSVSKKDHFVVEGKVYCDTCRIQFFTRVSKWMHGAKVKLVC 60
           MAKSF  ++  LC L     +++    F +EG VYCDTCR  F T  ++++ GA+V++VC
Sbjct: 1   MAKSFVPLIAVLCVLVLPLAAMAVGTPFHIEGSVYCDTCRFGFETIATQYIRGARVRIVC 60

Query: 61  REEEGGSETLAVEAETGKNGEYTIKVDGDHEEEVCEVNLVKSTDDDCAEIPTDGFGHRAR 120
           ++       L   A TG +G+Y + V GD +++ C   LV S    C E   D     A 
Sbjct: 61  KDRVTLKSELVGVAVTGPDGKYKVAVRGDRQDQQCLAELVHSPLSRCQE--ADPGRSTAT 120

Query: 121 VSITANDGITNPVRQPNPLGFMKKETLPQC 151
           V +T ++G  +     N +GF + E L  C
Sbjct: 121 VILTRSNGAASTRHYANAMGFFRDEPLRGC 148

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O498131.6e-4355.90Olee1-like protein OS=Betula pendula OX=3505 PE=2 SV=1[more]
Q8LGR03.7e-3749.38Pollen allergen Che a 1 OS=Chenopodium album OX=3559 PE=1 SV=1[more]
Q29W253.2e-3648.75Pollen allergen Cro s 1 OS=Crocus sativus OX=82528 PE=1 SV=1[more]
E2D0Z04.6e-3555.81Pollen allergen Sal k 5.0101 (Fragment) OS=Kali turgidum OX=151250 PE=1 SV=1[more]
P134475.6e-3348.10Anther-specific protein LAT52 OS=Solanum lycopersicum OX=4081 GN=LAT52 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
AT1G29140.12.5e-3648.17Pollen Ole e 1 allergen and extensin family protein [more]
AT5G45880.13.0e-3451.11Pollen Ole e 1 allergen and extensin family protein [more]
AT4G18596.16.7e-3445.28Pollen Ole e 1 allergen and extensin family protein [more]
AT4G08685.16.3e-2438.31Pollen Ole e 1 allergen and extensin family protein [more]
AT5G10130.15.2e-1834.00Pollen Ole e 1 allergen and extensin family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availablePFAMPF01190Pollen_Ole_e_1coord: 30..111
e-value: 1.4E-16
score: 60.7
NoneNo IPR availablePANTHERPTHR31614:SF2F28N24.16 PROTEINcoord: 4..162
IPR006041Pollen allergen Ole e 1 familyPANTHERPTHR31614PROTEIN DOWNSTREAM OF FLC-RELATEDcoord: 4..162
IPR006040Allergen Ole e 1, conserved sitePROSITEPS00925OLEEIcoord: 31..40

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G008800.1CmaCh11G008800.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0005615 extracellular space