CmaCh11G008200 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh11G008200
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionFormyltetrahydrofolate synthetase
LocationCma_Chr11: 4024415 .. 4028982 (+)
RNA-Seq ExpressionCmaCh11G008200
SyntenyCmaCh11G008200
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
TCCGCCATACCATCCCTAAATGACAACAGTTCACTGGCCCGCACTTCGATGGTTCTTAAAAAACTCGTCCACCAATTTGAATCTTCAACTTTCATCCATCAATAATTTCTTTAATCCGTAAAACAATGCATTTTTTTCTTTCTGGAAAGAGCTTGATTGCGACGTTTTTGACAATCTTATCCATTCCTTCCACTGAGATAGATCCGTAGCGACTATTTGAAGAAGAATTTCAATCCCGCGGAAACGCATTTTTGGAGAAGGAAGCTCGTTTCTGTTCTGGTTTTGTGAGATTTTGTTATAGTTTTGCAATATGAGTTCGTCTAAGACAGTGAGGAAACTTCAGGTGGAGTCTCCAGTGCCTGCGGATATAGTCATTGCCAATTCTGTGGCGCCGCTGCACATCGATGATATTGCTAAAGATCTCAACCTTAGCCCCACTCATTATGATCTTTATGGCAAATACAAGGCCAAGGTGATTCTGATTTGAGTGATTCTATGTTCTAGTTTGTTTTGATTGCCTGATTAGGAAAATGGTCGTAAAATTGTGTTGTCTTTGATCTTTGTTTAGGAAATAAAGTTGAATTGAATTCTAAACTGTTTGGAGGGCCAAATCTGAGGGTGTAATTATGTGTGGATTCAATTGTGTTGGTGTTTGGTGTTTGGTTTGCAGGTATTGTTGTCTGTTCTTGATGAGCTTAAAGGATCTAAGGATGGATATTATGTGGTGGTTGGGGGGATTACTCCTACTCCTCTTGGGGAAGGCAAATCCACCACAACTGTCGGTCTCTGTCAAGCTCTTGGTGCTTTCCTTGAAAAAAAGGTAATTTTCTCTCTTTTTAGATTGAGTTGTATGATTGCATGTTTGATTTCATTTTCAAGTAGTTTTGTTGGTGCACCTCGACACTGCTCGATAACCATTTGATTTTTAAGAATTAGCCTACAAATACTACTTCTACTGATGAATTTAGGTGTTTTTGTAACATTCCAAATCTGCCACTAGCGGATAGTGTCCTTTTTGGCTCGTTACGTACCGCCATTAACCTCACGGTTTTAAAACACATGTATTAGGGAGAGATTTCCATACCCTTATAAGGAGTGTTTCGGTCTCCTCTCCAACCCTTATAAGGAGTGTCTCCAACCCTTATAAGGAGTGTTTCCATACCCTTATAAGGAGGTTTCCATTCCCTTATGTGGGATCTCACCGGGAAACAAAGCATTCTTTATAAGGGTGTGGAAATCTCTCCCTAACAGAAGCGTTCGAAAACCTTGAGGGAAAGCTCAGAAGGGAAAGCCAAAAGAAGACAATATGAGCTTGAGCTGTTACAGTTATGTTATCTACTTTCTGCATATGTTCTAAAAAACAAAGTCAAGTTTTAAAGGAAAGGAAAACTAAGAATTCAAATGTTTTCTTAAGAAATATGAAACCAAACCATTGTAAAGAAATGATGAGAAAACAATTACAATTTCCAGAAATTAAAGTAAAAACTTAAATGGTTATCAAATATGACCGTAGTTGTTCATTTCTAAGATTACAGTTCAAATTCTGCAACACAAGGAGATGAAACATTCTATTTACTGTTGAGATTTGAACTTATCACCCAATGGGGTTGTATGGGTAAAAAGATCCTAAACTAATAATTGTATGAATTTAAACTCCGAACTTTCATGAGAGTATCAGTTCATAGACAAATTTTGCTTAAAAAACGTTAATCTGCACATGTGCAAACTTATAATTGTTTAGGCTGATTTCAGGTGGTCACCTGCCTTCGCCAGCCATCACAGGGACCAACATTTGGAATAAAAGGTGGTGCAGCTGGAGGGGGTTACAGTCAAGTGATTCCAATGGACGAATTTAATCTCCACCTAACAGGAGATATTCATGCTATAACAGCTGCAAACAATCTTTTAGCTGCTGCTATTGATACCAGGATTTTCCATGAGTCAACACAGTCAGATAAGGCTCTTTTTAATCGCCTTTGCCCGCCGAACAAAGAAGGTAAACGAAGCTTTAGTGATATCATGTTCAGACGTTTGAAGAAACTCAACATTGCCAAGACAAAACCAGAAGATCTGACACCCGAAGAAGTTACGAAGTTTGCCAGGCTCGATATTGATCCAGATTCTATTACCTGGAGAAGAGTAATGGACGTAAATGATCGGTTCTTAAGGAAAATTTCTATTGGTCAGGGTCCTGATGAGAAAGGGATGGTGAGAGAAACTGGGTTTGATATTTCAGTTGCCAGTGAAATAATGGCAGTTTTGGCCCTTACAACATCCCTGGCTGATATGAGAGAGAGGTTAGGGAAAATGGTAGTAGGAAACAGCAAGGCTGGTGACCCTGTAACTGCTGATGATCTCGGTGTCGGTGGCGCTTTGACCGTGCTCATGAAGGATGCTATTAACCCGACACTAATGCAGACTCTTGAGGGAACTCCTGTTCTTGTTCATGCTGGTCCTTTTGCTAATATTGCTCACGGTAATTCCTCTATAGTGGCTGATAAGATAGCTCTGAAGTTGGTAGGACCAGGTGGGTTTGTAGTCACAGAAGCAGGTTTCGGGGCCGATATTGGTGCCGAGAAATTTATGAACATCAAATGTCGCTATAGCGGTTTAACTCCACAATGTGCTGTAATTGTGGCGACGATAAGAGCGTTGAAAATGCATGGGGGCGGGCCACAAGTTGTTGCAGGGAGACCTCTTGACCATGCCTATTTGAATGAAAATGTGAGTCTTGTTGAAGCTGGCTGTGTTAATTTGGCCAGGCATATTGCAAACACCAAGGCTTATGGTGTGAATGTAGTTGTTGCTGTGAACAAGTTTGCCACAGATACTGAAGCAGAGATGAATGCAGTCAAAAGCGCTGCCTTAGCTGCCGGGGCGTACGATGCTGTTATTTGTTCTCATCACGCTCACGGTGGAAAAGGAGCGGTAAGACACTCGATCCCGATAATTAATAATCTTTTAGACAACCAACCCTGTAAAATGCAAAAGTGATGTTGTCTTTGATGACAAATTATCATGTTCTTTTGATTGGGTAGTAGACTTTATCACGTTCTTCATTTCTGTAGGTCGACCTCGGAATTGCAGTTCAAAAAGCGTGTGAGAACGTAACAAAGCCATTGAATTTCTTATATCCGCTTGATGTTGGTATTAAATACAAAATAGAAGCTATAGCCAAGTCCTATGGAGCCAGTGGGGTGGAATACTCTGAACAGGTGAGCTTTTGTTGCTATTACTATATATTGTTCGCATGCTATCAGCTAAGAAATCGTTTGATTTGCGTACCATATTTTGAATGATTGATGCAATGATATCATAGATTCTTGTTTCTTTGACTGAAATAATCCATCTAAGAACAGTAAGACAATGAACAGTAAGTGCTGGACTCCCAAATCTTAAGACATGTACAATTGAAGATACAAAGTTTGAGCTTCCTTGATAAAAAGAAACTAAACCCAAGTTCATCCATGTTAGTTCAGTTCAGTTCAACAAAATAAGGACGATCTTCAACCGTGTTGAATCGATCTTTCACTACGGGAAGCATCGGATCCTGTAGTTATTGATTTACCTTTATGTTTACTTATATGAGTTGCCCGCGAATCCTGCTAGTTCCTAGGCCATCAACCCCTATCAAAACTCCAATCAACAACTGAGGGCGACTCAAAGAGGCACTGCTAGAGGAGCCAGAGGGCCAAGCGGCTAAATTTCTCCAAGAGTCCTATTATTCCTCTTGGGAAAAGACTTTGATCCTAGTTGTAAGGCTCGGATATGGTGCCATCATTACTAAAAACTTAAAAAACTGAACATTAAATTAGTTAAAACTCCGATCTCCTAGCATTTCCTTCTGCAGTTGAGAATACAATTGGTTGATGTGAAAGAGTTTCTGTATAGAAACACTGATAAGAAACTGAAAAAGGTTTACTGTTTGAATCACAGGCGGAGAAACAGATTGAGATGTACAGTAGGCAAGGATTTTCAAATCTACCAATCTGCATGGCAAAAACACAATACTCCTTCTCACACGATGCTGCATTAAAGGGAGCGCCGAGCGGATTCAAGTTGCCGATAAGGGACATCAGGGCTAGCATTGGTGCTGGCTTCATTTACCCTTTGGTGGGAACCATGAGTACAATGCCAGGACTCCCTACAAGACCTTGCTTTTACGACATCGATCTCGACACCGCTACTGGGAAGGTCATTGGTCTTTCTTGAATGAGCCTTCCTCCCAAGCAGTTGCCTCGCCATCTTTCTGGTTTGTTGAACGGCCTGCCTCAAAAAAGTTCTTGTCCAAGTACATGTTTTTTTTGTTTAGTTTTCTCAACTGTTGTTATTCCGGTCTATCCTGTAATGCAACCTTTGCTGAACTTTGAACAGTCTGAAGTTATATGTCCTTAATAAAGTGGAAGGAGGGAAATAATGTGTGTGTGTTGTAGCGTGTATTTCCCTTCCTTTGTTTAGAATATATCTATTTTGATGGAGAATAATAGAAAGAGCCATTTTCAAATTGGTGGTAGATCCTTCCTTTTAGACCTTATGCCTTTATACACTACATATTCTTTTTTGACCTG

mRNA sequence

TCCGCCATACCATCCCTAAATGACAACAGTTCACTGGCCCGCACTTCGATGGTTCTTAAAAAACTCGTCCACCAATTTGAATCTTCAACTTTCATCCATCAATAATTTCTTTAATCCGTAAAACAATGCATTTTTTTCTTTCTGGAAAGAGCTTGATTGCGACGTTTTTGACAATCTTATCCATTCCTTCCACTGAGATAGATCCGTAGCGACTATTTGAAGAAGAATTTCAATCCCGCGGAAACGCATTTTTGGAGAAGGAAGCTCGTTTCTGTTCTGGTTTTGTGAGATTTTGTTATAGTTTTGCAATATGAGTTCGTCTAAGACAGTGAGGAAACTTCAGGTGGAGTCTCCAGTGCCTGCGGATATAGTCATTGCCAATTCTGTGGCGCCGCTGCACATCGATGATATTGCTAAAGATCTCAACCTTAGCCCCACTCATTATGATCTTTATGGCAAATACAAGGCCAAGGTATTGTTGTCTGTTCTTGATGAGCTTAAAGGATCTAAGGATGGATATTATGTGGTGGTTGGGGGGATTACTCCTACTCCTCTTGGGGAAGGCAAATCCACCACAACTGTCGGTCTCTGTCAAGCTCTTGGTGCTTTCCTTGAAAAAAAGGTGGTCACCTGCCTTCGCCAGCCATCACAGGGACCAACATTTGGAATAAAAGGTGGTGCAGCTGGAGGGGGTTACAGTCAAGTGATTCCAATGGACGAATTTAATCTCCACCTAACAGGAGATATTCATGCTATAACAGCTGCAAACAATCTTTTAGCTGCTGCTATTGATACCAGGATTTTCCATGAGTCAACACAGTCAGATAAGGCTCTTTTTAATCGCCTTTGCCCGCCGAACAAAGAAGGTAAACGAAGCTTTAGTGATATCATGTTCAGACGTTTGAAGAAACTCAACATTGCCAAGACAAAACCAGAAGATCTGACACCCGAAGAAGTTACGAAGTTTGCCAGGCTCGATATTGATCCAGATTCTATTACCTGGAGAAGAGTAATGGACGTAAATGATCGGTTCTTAAGGAAAATTTCTATTGGTCAGGGTCCTGATGAGAAAGGGATGGTGAGAGAAACTGGGTTTGATATTTCAGTTGCCAGTGAAATAATGGCAGTTTTGGCCCTTACAACATCCCTGGCTGATATGAGAGAGAGGTTAGGGAAAATGGTAGTAGGAAACAGCAAGGCTGGTGACCCTGTAACTGCTGATGATCTCGGTGTCGGTGGCGCTTTGACCGTGCTCATGAAGGATGCTATTAACCCGACACTAATGCAGACTCTTGAGGGAACTCCTGTTCTTGTTCATGCTGGTCCTTTTGCTAATATTGCTCACGGTAATTCCTCTATAGTGGCTGATAAGATAGCTCTGAAGTTGGTAGGACCAGGTGGGTTTGTAGTCACAGAAGCAGGTTTCGGGGCCGATATTGGTGCCGAGAAATTTATGAACATCAAATGTCGCTATAGCGGTTTAACTCCACAATGTGCTGTAATTGTGGCGACGATAAGAGCGTTGAAAATGCATGGGGGCGGGCCACAAGTTGTTGCAGGGAGACCTCTTGACCATGCCTATTTGAATGAAAATGTGAGTCTTGTTGAAGCTGGCTGTGTTAATTTGGCCAGGCATATTGCAAACACCAAGGCTTATGGTGTGAATGTAGTTGTTGCTGTGAACAAGTTTGCCACAGATACTGAAGCAGAGATGAATGCAGTCAAAAGCGCTGCCTTAGCTGCCGGGGCGTACGATGCTGTTATTTGTTCTCATCACGCTCACGGTGGAAAAGGAGCGGTCGACCTCGGAATTGCAGTTCAAAAAGCGTGTGAGAACGTAACAAAGCCATTGAATTTCTTATATCCGCTTGATGTTGGTATTAAATACAAAATAGAAGCTATAGCCAAGTCCTATGGAGCCAGTGGGGTGGAATACTCTGAACAGGCGGAGAAACAGATTGAGATGTACAGTAGGCAAGGATTTTCAAATCTACCAATCTGCATGGCAAAAACACAATACTCCTTCTCACACGATGCTGCATTAAAGGGAGCGCCGAGCGGATTCAAGTTGCCGATAAGGGACATCAGGGCTAGCATTGGTGCTGGCTTCATTTACCCTTTGGTGGGAACCATGAGTACAATGCCAGGACTCCCTACAAGACCTTGCTTTTACGACATCGATCTCGACACCGCTACTGGGAAGGTCATTGGTCTTTCTTGAATGAGCCTTCCTCCCAAGCAGTTGCCTCGCCATCTTTCTGGTTTGTTGAACGGCCTGCCTCAAAAAAGTTCTTGTCCAAGTACATGTTTTTTTTGTTTAGTTTTCTCAACTGTTGTTATTCCGGTCTATCCTGTAATGCAACCTTTGCTGAACTTTGAACAGTCTGAAGTTATATGTCCTTAATAAAGTGGAAGGAGGGAAATAATGTGTGTGTGTTGTAGCGTGTATTTCCCTTCCTTTGTTTAGAATATATCTATTTTGATGGAGAATAATAGAAAGAGCCATTTTCAAATTGGTGGTAGATCCTTCCTTTTAGACCTTATGCCTTTATACACTACATATTCTTTTTTGACCTG

Coding sequence (CDS)

ATGAGTTCGTCTAAGACAGTGAGGAAACTTCAGGTGGAGTCTCCAGTGCCTGCGGATATAGTCATTGCCAATTCTGTGGCGCCGCTGCACATCGATGATATTGCTAAAGATCTCAACCTTAGCCCCACTCATTATGATCTTTATGGCAAATACAAGGCCAAGGTATTGTTGTCTGTTCTTGATGAGCTTAAAGGATCTAAGGATGGATATTATGTGGTGGTTGGGGGGATTACTCCTACTCCTCTTGGGGAAGGCAAATCCACCACAACTGTCGGTCTCTGTCAAGCTCTTGGTGCTTTCCTTGAAAAAAAGGTGGTCACCTGCCTTCGCCAGCCATCACAGGGACCAACATTTGGAATAAAAGGTGGTGCAGCTGGAGGGGGTTACAGTCAAGTGATTCCAATGGACGAATTTAATCTCCACCTAACAGGAGATATTCATGCTATAACAGCTGCAAACAATCTTTTAGCTGCTGCTATTGATACCAGGATTTTCCATGAGTCAACACAGTCAGATAAGGCTCTTTTTAATCGCCTTTGCCCGCCGAACAAAGAAGGTAAACGAAGCTTTAGTGATATCATGTTCAGACGTTTGAAGAAACTCAACATTGCCAAGACAAAACCAGAAGATCTGACACCCGAAGAAGTTACGAAGTTTGCCAGGCTCGATATTGATCCAGATTCTATTACCTGGAGAAGAGTAATGGACGTAAATGATCGGTTCTTAAGGAAAATTTCTATTGGTCAGGGTCCTGATGAGAAAGGGATGGTGAGAGAAACTGGGTTTGATATTTCAGTTGCCAGTGAAATAATGGCAGTTTTGGCCCTTACAACATCCCTGGCTGATATGAGAGAGAGGTTAGGGAAAATGGTAGTAGGAAACAGCAAGGCTGGTGACCCTGTAACTGCTGATGATCTCGGTGTCGGTGGCGCTTTGACCGTGCTCATGAAGGATGCTATTAACCCGACACTAATGCAGACTCTTGAGGGAACTCCTGTTCTTGTTCATGCTGGTCCTTTTGCTAATATTGCTCACGGTAATTCCTCTATAGTGGCTGATAAGATAGCTCTGAAGTTGGTAGGACCAGGTGGGTTTGTAGTCACAGAAGCAGGTTTCGGGGCCGATATTGGTGCCGAGAAATTTATGAACATCAAATGTCGCTATAGCGGTTTAACTCCACAATGTGCTGTAATTGTGGCGACGATAAGAGCGTTGAAAATGCATGGGGGCGGGCCACAAGTTGTTGCAGGGAGACCTCTTGACCATGCCTATTTGAATGAAAATGTGAGTCTTGTTGAAGCTGGCTGTGTTAATTTGGCCAGGCATATTGCAAACACCAAGGCTTATGGTGTGAATGTAGTTGTTGCTGTGAACAAGTTTGCCACAGATACTGAAGCAGAGATGAATGCAGTCAAAAGCGCTGCCTTAGCTGCCGGGGCGTACGATGCTGTTATTTGTTCTCATCACGCTCACGGTGGAAAAGGAGCGGTCGACCTCGGAATTGCAGTTCAAAAAGCGTGTGAGAACGTAACAAAGCCATTGAATTTCTTATATCCGCTTGATGTTGGTATTAAATACAAAATAGAAGCTATAGCCAAGTCCTATGGAGCCAGTGGGGTGGAATACTCTGAACAGGCGGAGAAACAGATTGAGATGTACAGTAGGCAAGGATTTTCAAATCTACCAATCTGCATGGCAAAAACACAATACTCCTTCTCACACGATGCTGCATTAAAGGGAGCGCCGAGCGGATTCAAGTTGCCGATAAGGGACATCAGGGCTAGCATTGGTGCTGGCTTCATTTACCCTTTGGTGGGAACCATGAGTACAATGCCAGGACTCCCTACAAGACCTTGCTTTTACGACATCGATCTCGACACCGCTACTGGGAAGGTCATTGGTCTTTCTTGA

Protein sequence

MSSSKTVRKLQVESPVPADIVIANSVAPLHIDDIAKDLNLSPTHYDLYGKYKAKVLLSVLDELKGSKDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFLEKKVVTCLRQPSQGPTFGIKGGAAGGGYSQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQSDKALFNRLCPPNKEGKRSFSDIMFRRLKKLNIAKTKPEDLTPEEVTKFARLDIDPDSITWRRVMDVNDRFLRKISIGQGPDEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGKMVVGNSKAGDPVTADDLGVGGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGPGGFVVTEAGFGADIGAEKFMNIKCRYSGLTPQCAVIVATIRALKMHGGGPQVVAGRPLDHAYLNENVSLVEAGCVNLARHIANTKAYGVNVVVAVNKFATDTEAEMNAVKSAALAAGAYDAVICSHHAHGGKGAVDLGIAVQKACENVTKPLNFLYPLDVGIKYKIEAIAKSYGASGVEYSEQAEKQIEMYSRQGFSNLPICMAKTQYSFSHDAALKGAPSGFKLPIRDIRASIGAGFIYPLVGTMSTMPGLPTRPCFYDIDLDTATGKVIGLS
Homology
BLAST of CmaCh11G008200 vs. ExPASy Swiss-Prot
Match: P28723 (Formate--tetrahydrofolate ligase OS=Spinacia oleracea OX=3562 PE=1 SV=3)

HSP 1 Score: 1147.9 bits (2968), Expect = 0.0e+00
Identity = 568/634 (89.59%), Postives = 606/634 (95.58%), Query Frame = 0

Query: 3   SSKTVRKLQVESPVPADIVIANSVAPLHIDDIAKDLNLSPTHYDLYGKYKAKVLLSVLDE 62
           SSKTVRKL+V +PVPADI IAN+V PLHI DIA DLNLSP HYDLYGKYKAKVLLSVLDE
Sbjct: 4   SSKTVRKLEVATPVPADIDIANAVEPLHIADIAADLNLSPRHYDLYGKYKAKVLLSVLDE 63

Query: 63  LKGSKDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFLEKKVVTCLRQPSQGPTFGIKG 122
           ++ ++DGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFL+KKVVTCLRQPSQGPTFGIKG
Sbjct: 64  VQETQDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFLDKKVVTCLRQPSQGPTFGIKG 123

Query: 123 GAAGGGYSQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQSDKALFNRLCPP 182
           GAAGGGYSQVIPMDEFNLHLTGDIHAITA+NNLLAAAIDTR+FHESTQSDKALFNRLCPP
Sbjct: 124 GAAGGGYSQVIPMDEFNLHLTGDIHAITASNNLLAAAIDTRMFHESTQSDKALFNRLCPP 183

Query: 183 NKEGKRSFSDIMFRRLKKLNIAKTKPEDLTPEEVTKFARLDIDPDSITWRRVMDVNDRFL 242
           NKEGKR+F +IM RRLKKL I KT P+DLTPEEVTKFARLDIDPDSITWRRVMDVNDRFL
Sbjct: 184 NKEGKRTFCNIMHRRLKKLGIDKTNPDDLTPEEVTKFARLDIDPDSITWRRVMDVNDRFL 243

Query: 243 RKISIGQGPDEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGKMVVGNSKAGDPVT 302
           RKIS+GQGPDEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGKMV+GNSKAG+P+T
Sbjct: 244 RKISVGQGPDEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGKMVIGNSKAGEPIT 303

Query: 303 ADDLGVGGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGP 362
           ADDLG+GGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGP
Sbjct: 304 ADDLGLGGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGP 363

Query: 363 GGFVVTEAGFGADIGAEKFMNIKCRYSGLTPQCAVIVATIRALKMHGGGPQVVAGRPLDH 422
           GGFVVTEAGFG+DIG EKFMNIKCRYSGLTPQCA++VAT+RALKMHGGGPQVVAG+PLD 
Sbjct: 364 GGFVVTEAGFGSDIGTEKFMNIKCRYSGLTPQCAIVVATVRALKMHGGGPQVVAGKPLDR 423

Query: 423 AYLNENVSLVEAGCVNLARHIANTKAYGVNVVVAVNKFATDTEAEMNAVKSAALAAGAYD 482
           AYL ENV LVEAGCVNLARHI NTKAYG NVVVA+N F++DTEAE+NAVK AA+ AGA+D
Sbjct: 424 AYLTENVGLVEAGCVNLARHIINTKAYGSNVVVAINMFSSDTEAELNAVKKAAMDAGAFD 483

Query: 483 AVICSHHAHGGKGAVDLGIAVQKACENVTKPLNFLYPLDVGIKYKIEAIAKSYGASGVEY 542
           AVIC+HHAHGGKGAVDLGIAVQKACENVT+PL FLYPLD+ IK KIEAIAKSYGA+GVEY
Sbjct: 484 AVICTHHAHGGKGAVDLGIAVQKACENVTQPLRFLYPLDISIKEKIEAIAKSYGAAGVEY 543

Query: 543 SEQAEKQIEMYSRQGFSNLPICMAKTQYSFSHDAALKGAPSGFKLPIRDIRASIGAGFIY 602
           SEQAEK+IEMYS+QGFSNLPICMAKTQYSFSH+AA KGAPSGF LPIRD+R SIGAGFIY
Sbjct: 544 SEQAEKKIEMYSKQGFSNLPICMAKTQYSFSHNAAEKGAPSGFILPIRDVRGSIGAGFIY 603

Query: 603 PLVGTMSTMPGLPTRPCFYDIDLDTATGKVIGLS 637
           PLVGTMSTMPGLPTRPCF+DIDLDT TGKVIGLS
Sbjct: 604 PLVGTMSTMPGLPTRPCFFDIDLDTTTGKVIGLS 637

BLAST of CmaCh11G008200 vs. ExPASy Swiss-Prot
Match: Q9SPK5 (Formate--tetrahydrofolate ligase OS=Arabidopsis thaliana OX=3702 GN=THFS PE=2 SV=1)

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 554/633 (87.52%), Postives = 601/633 (94.94%), Query Frame = 0

Query: 4   SKTVRKLQVESPVPADIVIANSVAPLHIDDIAKDLNLSPTHYDLYGKYKAKVLLSVLDEL 63
           S + RKL+V SPVPADI IANSV PLHI +IAKDLN++P HYDLYGKYKAKVLLS  DEL
Sbjct: 2   SSSTRKLEVVSPVPADIDIANSVEPLHISEIAKDLNINPLHYDLYGKYKAKVLLSAFDEL 61

Query: 64  KGSKDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFLEKKVVTCLRQPSQGPTFGIKGG 123
           +G +DGYYVVVGGITPTPLGEGKSTTTVGLCQALGA+L+KKVVTCLRQPSQGPTFGIKGG
Sbjct: 62  QGQEDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAYLDKKVVTCLRQPSQGPTFGIKGG 121

Query: 124 AAGGGYSQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQSDKALFNRLCPPN 183
           AAGGGYSQVIPMDEFNLHLTGDIHAITA+NNLLAAAIDTRIFHE++QSDKALFNRLCPPN
Sbjct: 122 AAGGGYSQVIPMDEFNLHLTGDIHAITASNNLLAAAIDTRIFHETSQSDKALFNRLCPPN 181

Query: 184 KEGKRSFSDIMFRRLKKLNIAKTKPEDLTPEEVTKFARLDIDPDSITWRRVMDVNDRFLR 243
           KEGKRSFSDIMFRRL KL I+KT PE+LTPEE+ KFARLDIDP SITWRRVMDVNDRFLR
Sbjct: 182 KEGKRSFSDIMFRRLTKLGISKTSPEELTPEEIKKFARLDIDPASITWRRVMDVNDRFLR 241

Query: 244 KISIGQGPDEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGKMVVGNSKAGDPVTA 303
           KI+IGQGP+EKGM RETGFDISVASEIMAVLALTTSL DMRERLGKMV+GNSKAGDP+TA
Sbjct: 242 KITIGQGPEEKGMTRETGFDISVASEIMAVLALTTSLGDMRERLGKMVIGNSKAGDPITA 301

Query: 304 DDLGVGGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGPG 363
           DDLGVGGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGPG
Sbjct: 302 DDLGVGGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGPG 361

Query: 364 GFVVTEAGFGADIGAEKFMNIKCRYSGLTPQCAVIVATIRALKMHGGGPQVVAGRPLDHA 423
           GFVVTEAGFG+DIG EKFMNIKCRYSGLTPQCA++VAT+RALKMHGGGP VVAGRPLD A
Sbjct: 362 GFVVTEAGFGSDIGTEKFMNIKCRYSGLTPQCAIVVATVRALKMHGGGPDVVAGRPLDRA 421

Query: 424 YLNENVSLVEAGCVNLARHIANTKAYGVNVVVAVNKFATDTEAEMNAVKSAALAAGAYDA 483
           Y++ENVSLVEAGCVNLA+HI+NTKAYGVNV+VAVN FATDTEAE+NAV+  ++ AGA+DA
Sbjct: 422 YVSENVSLVEAGCVNLAKHISNTKAYGVNVIVAVNMFATDTEAELNAVRKFSMDAGAFDA 481

Query: 484 VICSHHAHGGKGAVDLGIAVQKACENVTKPLNFLYPLDVGIKYKIEAIAKSYGASGVEYS 543
           V+CSHHAH GKGAVDLGIAV+KAC+N+T+PL FLYPLD+GIK KIEAIAKSYGASGVEYS
Sbjct: 482 VVCSHHAHSGKGAVDLGIAVEKACQNITQPLRFLYPLDIGIKDKIEAIAKSYGASGVEYS 541

Query: 544 EQAEKQIEMYSRQGFSNLPICMAKTQYSFSHDAALKGAPSGFKLPIRDIRASIGAGFIYP 603
           +QAEKQIEMY++QGFSNLPICM+KTQYSFSHDA+ KGAPSGF LPIRD+R SIGAGFIYP
Sbjct: 542 DQAEKQIEMYTQQGFSNLPICMSKTQYSFSHDASKKGAPSGFVLPIRDVRGSIGAGFIYP 601

Query: 604 LVGTMSTMPGLPTRPCFYDIDLDTATGKVIGLS 637
           LVGTMSTMPGLPTRPCFY+ID+DT TGKV GLS
Sbjct: 602 LVGTMSTMPGLPTRPCFYEIDIDTETGKVRGLS 634

BLAST of CmaCh11G008200 vs. ExPASy Swiss-Prot
Match: P11586 (C-1-tetrahydrofolate synthase, cytoplasmic OS=Homo sapiens OX=9606 GN=MTHFD1 PE=1 SV=4)

HSP 1 Score: 823.9 bits (2127), Expect = 1.2e-237
Identity = 425/627 (67.78%), Postives = 491/627 (78.31%), Query Frame = 0

Query: 10  LQVESPVPADIVIANSVAPLHIDDIAKDLNLSPTHYDLYGKYKAKVLLSVLDELKGSKDG 69
           L +++PVP+DI I+ S  P  I  +A+++ L     +LYG+ KAKVLLS L+ LK   DG
Sbjct: 310 LNLKTPVPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKHRPDG 369

Query: 70  YYVVVGGITPTPLGEGKSTTTVGLCQALGAFLEKKVVTCLRQPSQGPTFGIKGGAAGGGY 129
            YVVV GITPTPLGEGKSTTT+GL QALGA L + V  C+RQPSQGPTFGIKGGAAGGGY
Sbjct: 370 KYVVVTGITPTPLGEGKSTTTIGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAAGGGY 429

Query: 130 SQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQSDKALFNRLCPPNKEGKRS 189
           SQVIPM+EFNLHLTGDIHAITAANNL+AAAID RIFHE TQ+DKALFNRL  P+  G R 
Sbjct: 430 SQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLV-PSVNGVRR 489

Query: 190 FSDIMFRRLKKLNIAKTKPEDLTPEEVTKFARLDIDPDSITWRRVMDVNDRFLRKISIGQ 249
           FSDI  RRLK+L I KT P  LT EE+ +FARLDIDP++ITW+RV+D NDRFLRKI+IGQ
Sbjct: 490 FSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQ 549

Query: 250 GPDEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGKMVVGNSKAGDPVTADDLGVG 309
            P EKG  R   FDISVASEIMAVLALTTSL DMRERLGKMVV +SK G+PV+A+DLGV 
Sbjct: 550 APTEKGHTRTAQFDISVASEIMAVLALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVS 609

Query: 310 GALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGPGGFVVTE 369
           GALTVLMKDAI P LMQTLEGTPV VHAGPFANIAHGNSSI+AD+IALKLVGP GFVVTE
Sbjct: 610 GALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTE 669

Query: 370 AGFGADIGAEKFMNIKCRYSGLTPQCAVIVATIRALKMHGGGPQVVAGRPLDHAYLNENV 429
           AGFGADIG EKF NIKCRYSGL P   V+VAT+RALKMHGGGP V AG PL  AY+ EN+
Sbjct: 670 AGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIQENL 729

Query: 430 SLVEAGCVNLARHIANTKAYGVNVVVAVNKFATDTEAEMNAVKSAALAAGAYDAVICSHH 489
            LVE G  NL + I N + +G+ VVVAVN F TDTE+E++ +   +   GA+DAV C+H 
Sbjct: 730 ELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTESELDLISRLSREHGAFDAVKCTHW 789

Query: 490 AHGGKGAVDLGIAVQKACENVTKPLNFLYPLDVGIKYKIEAIA-KSYGASGVEYSEQAEK 549
           A GGKGA+ L  AVQ+A +        LY L + ++ KI  IA K YGA  +E   +A+ 
Sbjct: 790 AEGGKGALALAQAVQRAAQ-APSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQH 849

Query: 550 QIEMYSRQGFSNLPICMAKTQYSFSHDAALKGAPSGFKLPIRDIRASIGAGFIYPLVGTM 609
           + E+Y++QGF NLPICMAKT  S SH+   KG P+GF LPIRDIRAS+GAGF+YPLVGTM
Sbjct: 850 KAEVYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTM 909

Query: 610 STMPGLPTRPCFYDIDLDTATGKVIGL 636
           STMPGLPTRPCFYDIDLD  T +V GL
Sbjct: 910 STMPGLPTRPCFYDIDLDPETEQVNGL 934

BLAST of CmaCh11G008200 vs. ExPASy Swiss-Prot
Match: Q922D8 (C-1-tetrahydrofolate synthase, cytoplasmic OS=Mus musculus OX=10090 GN=Mthfd1 PE=1 SV=4)

HSP 1 Score: 819.3 bits (2115), Expect = 3.0e-236
Identity = 421/628 (67.04%), Postives = 491/628 (78.18%), Query Frame = 0

Query: 9   KLQVESPVPADIVIANSVAPLHIDDIAKDLNLSPTHYDLYGKYKAKVLLSVLDELKGSKD 68
           KL +++PVP+DI I+ S  P  I ++A+++ L     +LYG+ KAKVLLS LD LK   D
Sbjct: 309 KLNLKTPVPSDIAISRSCKPKLIGNLAREIGLLTEEVELYGETKAKVLLSALDRLKHQPD 368

Query: 69  GYYVVVGGITPTPLGEGKSTTTVGLCQALGAFLEKKVVTCLRQPSQGPTFGIKGGAAGGG 128
           G YVVV GITPTPLGEGKSTTT+GL QALGA L + V  C+RQPSQGPTFGIKGGAAGGG
Sbjct: 369 GKYVVVTGITPTPLGEGKSTTTIGLVQALGAHLRQNVFACVRQPSQGPTFGIKGGAAGGG 428

Query: 129 YSQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQSDKALFNRLCPPNKEGKR 188
           YSQVIPM+EFNLHLTGDIHAITAANNL+AAAID RIFHE TQ+DKALFNRL  P+  G R
Sbjct: 429 YSQVIPMEEFNLHLTGDIHAITAANNLVAAAIDARIFHELTQTDKALFNRLV-PSVNGIR 488

Query: 189 SFSDIMFRRLKKLNIAKTKPEDLTPEEVTKFARLDIDPDSITWRRVMDVNDRFLRKISIG 248
            FSDI  RRL++L I KT P  LT +E+ +FARLDIDP++ITW+RV+D NDRFLRKI+IG
Sbjct: 489 KFSDIQIRRLRRLGIEKTDPTTLTDDEINRFARLDIDPETITWQRVLDTNDRFLRKITIG 548

Query: 249 QGPDEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGKMVVGNSKAGDPVTADDLGV 308
           Q P EKG  R   FDISVASEIMAVLALT+SL DMRERLG+MVV +SK G+P++ +DLGV
Sbjct: 549 QSPTEKGHTRTAQFDISVASEIMAVLALTSSLEDMRERLGRMVVASSKKGEPISCEDLGV 608

Query: 309 GGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGPGGFVVT 368
            GALTVLMKDAI P LMQTLEGTPV VHAGPFANIAHGNSSI+AD+IALKLVGP GFVVT
Sbjct: 609 SGALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVT 668

Query: 369 EAGFGADIGAEKFMNIKCRYSGLTPQCAVIVATIRALKMHGGGPQVVAGRPLDHAYLNEN 428
           EAGFGADIG EKF NIKCRYSGL P   V+VAT+RALKMHGGGP V AG PL  AY  E+
Sbjct: 669 EAGFGADIGMEKFFNIKCRYSGLQPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYTEED 728

Query: 429 VSLVEAGCVNLARHIANTKAYGVNVVVAVNKFATDTEAEMNAVKSAALAAGAYDAVICSH 488
           + LVE G  NL + I N + +GV VVVAVN F TDT+AE++ V   +   GA+DAV C+H
Sbjct: 729 LDLVEKGFSNLRKQIENARMFGVPVVVAVNVFKTDTDAELDLVSRLSREHGAFDAVKCTH 788

Query: 489 HAHGGKGAVDLGIAVQKACENVTKPLNFLYPLDVGIKYKIEAIA-KSYGASGVEYSEQAE 548
            A GG+GA+ L  AVQ+A +        LY L + I+ KI  IA + YGA  +E   +A+
Sbjct: 789 WAEGGQGALALAQAVQRASQ-APSSFQLLYDLKLSIEDKIRIIAQRIYGADDIELLPEAQ 848

Query: 549 KQIEMYSRQGFSNLPICMAKTQYSFSHDAALKGAPSGFKLPIRDIRASIGAGFIYPLVGT 608
            + E+Y++QGF NLPICMAKT  S SH+   KG P+GF LPIRDIRAS+GAGF+YPLVGT
Sbjct: 849 NKAEIYTKQGFGNLPICMAKTHLSLSHNPEQKGVPTGFVLPIRDIRASVGAGFLYPLVGT 908

Query: 609 MSTMPGLPTRPCFYDIDLDTATGKVIGL 636
           MSTMPGLPTRPCFYDIDLD  T +V GL
Sbjct: 909 MSTMPGLPTRPCFYDIDLDPETEQVNGL 934

BLAST of CmaCh11G008200 vs. ExPASy Swiss-Prot
Match: Q5R8P0 (C-1-tetrahydrofolate synthase, cytoplasmic OS=Pongo abelii OX=9601 GN=MTHFD1 PE=2 SV=3)

HSP 1 Score: 817.8 bits (2111), Expect = 8.7e-236
Identity = 422/627 (67.30%), Postives = 487/627 (77.67%), Query Frame = 0

Query: 10  LQVESPVPADIVIANSVAPLHIDDIAKDLNLSPTHYDLYGKYKAKVLLSVLDELKGSKDG 69
           L +++P P+DI I+ S  P  I  +A+++ L     +LYG+ KAKVLLS L+ LK   DG
Sbjct: 310 LNLKTPDPSDIDISRSCKPKPIGKLAREIGLLSEEVELYGETKAKVLLSALERLKHRPDG 369

Query: 70  YYVVVGGITPTPLGEGKSTTTVGLCQALGAFLEKKVVTCLRQPSQGPTFGIKGGAAGGGY 129
            YVVV GITPTPLGEGKSTTTVGL QALGA L + V  C+RQPSQGPTFGIKGGAAGGGY
Sbjct: 370 KYVVVTGITPTPLGEGKSTTTVGLVQALGAHLYQNVFACVRQPSQGPTFGIKGGAAGGGY 429

Query: 130 SQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQSDKALFNRLCPPNKEGKRS 189
           SQVIPM+EFNLHLTGDIHA TAANNL+AAAID RIFHE TQ+DKALFNRL  P+  G R 
Sbjct: 430 SQVIPMEEFNLHLTGDIHAFTAANNLVAAAIDARIFHELTQTDKALFNRLV-PSVNGVRK 489

Query: 190 FSDIMFRRLKKLNIAKTKPEDLTPEEVTKFARLDIDPDSITWRRVMDVNDRFLRKISIGQ 249
           FSDI  RRLK+L I KT P  LT EE+ +FARLDIDP++ITW+RV+D NDRFLRKI+IGQ
Sbjct: 490 FSDIQIRRLKRLGIEKTDPTTLTDEEINRFARLDIDPETITWQRVLDTNDRFLRKITIGQ 549

Query: 250 GPDEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGKMVVGNSKAGDPVTADDLGVG 309
            P EKG  R   FDISVASEIMA LALTTSL DMRERLGKMVV +SK G+PV+A+DLGV 
Sbjct: 550 APTEKGHTRTAQFDISVASEIMAALALTTSLEDMRERLGKMVVASSKKGEPVSAEDLGVS 609

Query: 310 GALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGPGGFVVTE 369
           GALTVLMKDAI P LMQTLEGTPV VHAGPFANIAHGNSSI+AD+IALKLVGP GFVVTE
Sbjct: 610 GALTVLMKDAIKPNLMQTLEGTPVFVHAGPFANIAHGNSSIIADRIALKLVGPEGFVVTE 669

Query: 370 AGFGADIGAEKFMNIKCRYSGLTPQCAVIVATIRALKMHGGGPQVVAGRPLDHAYLNENV 429
           AGFGADIG EKF NIKCRYSGL P   V+VAT+RALKMHGGGP V AG PL  AY+ EN+
Sbjct: 670 AGFGADIGMEKFFNIKCRYSGLCPHVVVLVATVRALKMHGGGPTVTAGLPLPKAYIEENL 729

Query: 430 SLVEAGCVNLARHIANTKAYGVNVVVAVNKFATDTEAEMNAVKSAALAAGAYDAVICSHH 489
            LVE G  NL + I N + +G+ VVVAVN F TDTEAE++ +   +   GA+DAV C+H 
Sbjct: 730 ELVEKGFSNLKKQIENARMFGIPVVVAVNAFKTDTEAELDLISRLSREHGAFDAVKCTHW 789

Query: 490 AHGGKGAVDLGIAVQKACENVTKPLNFLYPLDVGIKYKIEAIA-KSYGASGVEYSEQAEK 549
           A GG GA+ L  AVQ+A +        LY L + ++ KI  IA K YGA  +E   +A+ 
Sbjct: 790 AEGGNGALALAQAVQRAAQ-APSSFQLLYDLKLPVEDKIRIIAQKIYGADDIELLPEAQH 849

Query: 550 QIEMYSRQGFSNLPICMAKTQYSFSHDAALKGAPSGFKLPIRDIRASIGAGFIYPLVGTM 609
           + E+Y++QGF NLP+CMAKT  S SH+   KG P+GF LPIRDIRAS+GAGF+YPLVGTM
Sbjct: 850 KAEVYTKQGFGNLPVCMAKTHLSLSHNPEQKGVPTGFILPIRDIRASVGAGFLYPLVGTM 909

Query: 610 STMPGLPTRPCFYDIDLDTATGKVIGL 636
           STMPGLPTRPCFYDIDLD  T +V GL
Sbjct: 910 STMPGLPTRPCFYDIDLDPETQQVNGL 934

BLAST of CmaCh11G008200 vs. TAIR 10
Match: AT1G50480.1 (10-formyltetrahydrofolate synthetase )

HSP 1 Score: 1133.2 bits (2930), Expect = 0.0e+00
Identity = 554/633 (87.52%), Postives = 601/633 (94.94%), Query Frame = 0

Query: 4   SKTVRKLQVESPVPADIVIANSVAPLHIDDIAKDLNLSPTHYDLYGKYKAKVLLSVLDEL 63
           S + RKL+V SPVPADI IANSV PLHI +IAKDLN++P HYDLYGKYKAKVLLS  DEL
Sbjct: 2   SSSTRKLEVVSPVPADIDIANSVEPLHISEIAKDLNINPLHYDLYGKYKAKVLLSAFDEL 61

Query: 64  KGSKDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAFLEKKVVTCLRQPSQGPTFGIKGG 123
           +G +DGYYVVVGGITPTPLGEGKSTTTVGLCQALGA+L+KKVVTCLRQPSQGPTFGIKGG
Sbjct: 62  QGQEDGYYVVVGGITPTPLGEGKSTTTVGLCQALGAYLDKKVVTCLRQPSQGPTFGIKGG 121

Query: 124 AAGGGYSQVIPMDEFNLHLTGDIHAITAANNLLAAAIDTRIFHESTQSDKALFNRLCPPN 183
           AAGGGYSQVIPMDEFNLHLTGDIHAITA+NNLLAAAIDTRIFHE++QSDKALFNRLCPPN
Sbjct: 122 AAGGGYSQVIPMDEFNLHLTGDIHAITASNNLLAAAIDTRIFHETSQSDKALFNRLCPPN 181

Query: 184 KEGKRSFSDIMFRRLKKLNIAKTKPEDLTPEEVTKFARLDIDPDSITWRRVMDVNDRFLR 243
           KEGKRSFSDIMFRRL KL I+KT PE+LTPEE+ KFARLDIDP SITWRRVMDVNDRFLR
Sbjct: 182 KEGKRSFSDIMFRRLTKLGISKTSPEELTPEEIKKFARLDIDPASITWRRVMDVNDRFLR 241

Query: 244 KISIGQGPDEKGMVRETGFDISVASEIMAVLALTTSLADMRERLGKMVVGNSKAGDPVTA 303
           KI+IGQGP+EKGM RETGFDISVASEIMAVLALTTSL DMRERLGKMV+GNSKAGDP+TA
Sbjct: 242 KITIGQGPEEKGMTRETGFDISVASEIMAVLALTTSLGDMRERLGKMVIGNSKAGDPITA 301

Query: 304 DDLGVGGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGPG 363
           DDLGVGGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGPG
Sbjct: 302 DDLGVGGALTVLMKDAINPTLMQTLEGTPVLVHAGPFANIAHGNSSIVADKIALKLVGPG 361

Query: 364 GFVVTEAGFGADIGAEKFMNIKCRYSGLTPQCAVIVATIRALKMHGGGPQVVAGRPLDHA 423
           GFVVTEAGFG+DIG EKFMNIKCRYSGLTPQCA++VAT+RALKMHGGGP VVAGRPLD A
Sbjct: 362 GFVVTEAGFGSDIGTEKFMNIKCRYSGLTPQCAIVVATVRALKMHGGGPDVVAGRPLDRA 421

Query: 424 YLNENVSLVEAGCVNLARHIANTKAYGVNVVVAVNKFATDTEAEMNAVKSAALAAGAYDA 483
           Y++ENVSLVEAGCVNLA+HI+NTKAYGVNV+VAVN FATDTEAE+NAV+  ++ AGA+DA
Sbjct: 422 YVSENVSLVEAGCVNLAKHISNTKAYGVNVIVAVNMFATDTEAELNAVRKFSMDAGAFDA 481

Query: 484 VICSHHAHGGKGAVDLGIAVQKACENVTKPLNFLYPLDVGIKYKIEAIAKSYGASGVEYS 543
           V+CSHHAH GKGAVDLGIAV+KAC+N+T+PL FLYPLD+GIK KIEAIAKSYGASGVEYS
Sbjct: 482 VVCSHHAHSGKGAVDLGIAVEKACQNITQPLRFLYPLDIGIKDKIEAIAKSYGASGVEYS 541

Query: 544 EQAEKQIEMYSRQGFSNLPICMAKTQYSFSHDAALKGAPSGFKLPIRDIRASIGAGFIYP 603
           +QAEKQIEMY++QGFSNLPICM+KTQYSFSHDA+ KGAPSGF LPIRD+R SIGAGFIYP
Sbjct: 542 DQAEKQIEMYTQQGFSNLPICMSKTQYSFSHDASKKGAPSGFVLPIRDVRGSIGAGFIYP 601

Query: 604 LVGTMSTMPGLPTRPCFYDIDLDTATGKVIGLS 637
           LVGTMSTMPGLPTRPCFY+ID+DT TGKV GLS
Sbjct: 602 LVGTMSTMPGLPTRPCFYEIDIDTETGKVRGLS 634

BLAST of CmaCh11G008200 vs. TAIR 10
Match: AT2G12280.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 143.7 bits (361), Expect = 5.2e-34
Identity = 64/74 (86.49%), Postives = 71/74 (95.95%), Query Frame = 0

Query: 552 MYSRQGFSNLPICMAKTQYSFSHDAALKGAPSGFKLPIRDIRASIGAGFIYPLVGTMSTM 611
           MY++QGFSNLPICM+KTQYSFSHDA+ KGAPSGF LPIRD+R SIGAGFIYPLVGTMSTM
Sbjct: 1   MYTQQGFSNLPICMSKTQYSFSHDASKKGAPSGFVLPIRDVRGSIGAGFIYPLVGTMSTM 60

Query: 612 PGLPTRPCFYDIDL 626
           PGLPTRPCFY+ID+
Sbjct: 61  PGLPTRPCFYEIDI 74

BLAST of CmaCh11G008200 vs. TAIR 10
Match: AT2G12200.1 (P-loop containing nucleoside triphosphate hydrolases superfamily protein )

HSP 1 Score: 94.7 bits (234), Expect = 2.8e-19
Identity = 43/63 (68.25%), Postives = 52/63 (82.54%), Query Frame = 0

Query: 552 MYSRQGFSNLPICMAKTQYSFSHDAALKGAPSGFKLPIRDIRASIGAGFIYPLVGTMSTM 611
           MY++QGFSNLPICM+KTQYSFSHD + K APSGF LPIRD+R SIGAGFIY    T++ +
Sbjct: 1   MYTQQGFSNLPICMSKTQYSFSHDTSKKRAPSGFVLPIRDVRGSIGAGFIYTTKKTVNPL 60

Query: 612 PGL 615
            G+
Sbjct: 61  AGM 63

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
P287230.0e+0089.59Formate--tetrahydrofolate ligase OS=Spinacia oleracea OX=3562 PE=1 SV=3[more]
Q9SPK50.0e+0087.52Formate--tetrahydrofolate ligase OS=Arabidopsis thaliana OX=3702 GN=THFS PE=2 SV... [more]
P115861.2e-23767.78C-1-tetrahydrofolate synthase, cytoplasmic OS=Homo sapiens OX=9606 GN=MTHFD1 PE=... [more]
Q922D83.0e-23667.04C-1-tetrahydrofolate synthase, cytoplasmic OS=Mus musculus OX=10090 GN=Mthfd1 PE... [more]
Q5R8P08.7e-23667.30C-1-tetrahydrofolate synthase, cytoplasmic OS=Pongo abelii OX=9601 GN=MTHFD1 PE=... [more]
Match NameE-valueIdentityDescription
AT1G50480.10.0e+0087.5210-formyltetrahydrofolate synthetase [more]
AT2G12280.15.2e-3486.49P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
AT2G12200.12.8e-1968.25P-loop containing nucleoside triphosphate hydrolases superfamily protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR000559Formate-tetrahydrofolate ligase, FTHFSPFAMPF01268FTHFScoord: 18..636
e-value: 9.0E-255
score: 846.1
IPR000559Formate-tetrahydrofolate ligase, FTHFSHAMAPMF_01543FTHFScoord: 16..636
score: 40.741474
NoneNo IPR availableGENE3D3.10.410.10Formyltetrahydrofolate synthetase, domain 3coord: 523..611
e-value: 3.6E-35
score: 121.7
NoneNo IPR availableGENE3D1.10.8.770coord: 167..232
e-value: 1.4E-32
score: 113.9
NoneNo IPR availablePANTHERPTHR48099C-1-TETRAHYDROFOLATE SYNTHASE, CYTOPLASMIC-RELATEDcoord: 9..629
NoneNo IPR availablePANTHERPTHR48099:SF12MONOFUNCTIONAL C1-TETRAHYDROFOLATE SYNTHASE, MITOCHONDRIALcoord: 9..629
NoneNo IPR availableCDDcd00477FTHFScoord: 31..635
e-value: 0.0
score: 925.786
IPR027417P-loop containing nucleoside triphosphate hydrolaseGENE3D3.40.50.300coord: 271..522
e-value: 1.8E-85
score: 288.8
coord: 15..166
e-value: 7.5E-56
score: 191.4
IPR027417P-loop containing nucleoside triphosphate hydrolaseSUPERFAMILY52540P-loop containing nucleoside triphosphate hydrolasescoord: 19..633
IPR020628Formate-tetrahydrofolate ligase, FTHFS, conserved sitePROSITEPS00721FTHFS_1coord: 119..129
IPR020628Formate-tetrahydrofolate ligase, FTHFS, conserved sitePROSITEPS00722FTHFS_2coord: 399..410

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G008200.1CmaCh11G008200.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0035999 tetrahydrofolate interconversion
molecular_function GO:0005524 ATP binding
molecular_function GO:0004329 formate-tetrahydrofolate ligase activity
molecular_function GO:0016787 hydrolase activity
molecular_function GO:0016491 oxidoreductase activity