CmaCh11G006120 (gene) Cucurbita maxima (Rimu) v1.1

BLAST of CmaCh11G006120 vs. ExPASy Swiss-Prot
Match: A5ERG8 (tRNA(Ile)-lysidine synthase OS=Bradyrhizobium sp. (strain BTAi1 / ATCC BAA-1182) OX=288000 GN=tilS PE=3 SV=1)

HSP 1 Score: 128.6 bits (322), Expect = 2.9e-28
Identity = 81/205 (39.51%), Postives = 106/205 (51.71%), Query Frame = 0

Query: 83  IALGVSGGPDSMALCVLMAGWKTDSLNGTGKSSTFIGGLLAIVVDHGLRAESRDEANVVR 142
           + L VSGGPDSMAL  L A W+     G          LLA+ VDHGLR ESR EA +V+
Sbjct: 30  LTLAVSGGPDSMALMWLAARWRATRAGGP--------RLLAVTVDHGLRPESRREALMVK 89

Query: 143 NRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQMLQKVCVQHKIGVILVAHHADDQ 202
               Q+G+     +  W   KP  G + EAAR  RY++L +   Q     ++ AH  DDQ
Sbjct: 90  QLARQLGLTHR--TLRWSGEKPAAG-IPEAARIARYRLLARAAEQAGASHVVTAHTRDDQ 149

Query: 203 AELFILRLSRSSGVLGLAGMPFTSQIFSPHRYSYSEVSKNYGILLVRPLMEFSKDDLYKI 262
           AE  ++RL R SG+ GLA M              + VS+  G+LL RPL+  SK  L   
Sbjct: 150 AETVLMRLLRGSGIAGLAAM--------------APVSRRDGLLLARPLLSLSKARLLAT 209

Query: 263 CQGANQDWVEDPTNRSSLFARNRIR 288
            + A   + +DPTNR   F R R+R
Sbjct: 210 LRSAGIAFADDPTNRDPAFTRPRLR 209

BLAST of CmaCh11G006120 vs. ExPASy Swiss-Prot
Match: B3CLY6 (tRNA(Ile)-lysidine synthase OS=Wolbachia pipientis subsp. Culex pipiens (strain wPip) OX=570417 GN=tilS PE=3 SV=1)

HSP 1 Score: 124.0 bits (310), Expect = 7.3e-27
Identity = 96/328 (29.27%), Postives = 162/328 (49.39%), Query Frame = 0

Query: 80  HHRIALGVSGGPDSMALCVLMAGW-KTDSLNGTGKSSTFIGGLLAIVVDHGLRAESRDEA 139
           H++IA+ VSGG DS+ L  LM  W K + L+            +A+ V+HGLR+ES+ EA
Sbjct: 17  HNQIAVAVSGGVDSIVLLHLMTNWAKKNKLSLP----------IALTVNHGLRSESQKEA 76

Query: 140 NVVRNRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQMLQKVCVQHKIGVILVAHH 199
           + + +   ++G + E    +W + +  +G++Q  AR  RY++L + C  + +  +LVAHH
Sbjct: 77  DFIISYAKELGAK-ESFILNW-EKQNIKGNIQLQARKARYKLLAEWCKNNNVKCLLVAHH 136

Query: 200 ADDQAELFILRLSRSSGVLGLAGMPFTSQIFSPHRYSYSEVSKNYGILLVRPLMEFSKDD 259
            DDQAE F+LRL R SGV GL+ M + S +               GI + RPL+ FS+ +
Sbjct: 137 KDDQAETFLLRLERGSGVDGLSSMDYKSFL--------------NGIYIFRPLLNFSRSE 196

Query: 260 LYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDMSSCTLKSELHAIISACRKTRLFVDH 319
           + +  +     W+ED +N    + R   R  L    +  + +E   +      T L +  
Sbjct: 197 IERYAKLHQLRWIEDRSNYDLKYRRTLYRNLLKASDNQDVLTERICL------TALHMKR 256

Query: 320 ACLSLANLT-------LTITELGYAIINLEILNQLKIQDICLSKFLSLVLQFISQRHRPV 379
           A  +L + T       + + + GY  I L    QL  ++I L   L  ++   S+ ++P 
Sbjct: 257 AAKALMHYTRLAFDDCVNVHDFGYIEIKLSEFYQLP-EEIALRLLLYSIMAIASKHYKP- 310

Query: 380 RGNTAKLVLDYIQTVPCRTSLTAAGCYI 400
           R N+  ++ + I       + T +GC I
Sbjct: 317 RYNSLIVIFNKILQKGSNVNCTLSGCKI 310

BLAST of CmaCh11G006120 vs. ExPASy Swiss-Prot
Match: Q9XBG6 (tRNA(Ile)-lysidine synthase OS=Bradyrhizobium diazoefficiens (strain JCM 10833 / BCRC 13528 / IAM 13628 / NBRC 14792 / USDA 110) OX=224911 GN=tilS PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.2e-26
Identity = 78/221 (35.29%), Postives = 112/221 (50.68%), Query Frame = 0

Query: 75  AGLKPHHRIALGVSGGPDSMALCVLMAGWKTDSLNGTGKSSTFIGGLLAIVVDHGLRAES 134
           A LK    + L VSGGPDS+AL  L A W+     G          L  + VDHGLRAE+
Sbjct: 19  AELKSAPALVLAVSGGPDSIALMWLAARWQRSLARGP--------RLTVVTVDHGLRAEA 78

Query: 135 RDEANVVRNRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQMLQKVCVQHKIGVIL 194
             EA  V+   T++G+     +  W   KPK G L  AAR+ RY++L +         +L
Sbjct: 79  AREAREVKRLATELGL--PHRTLRWRGAKPKTG-LPAAAREARYRLLMQAARSAGASHVL 138

Query: 195 VAHHADDQAELFILRLSRSSGVLGLAGMPFTSQIFSPHRYSYSEVSKNYGILLVRPLMEF 254
            AH  DDQAE  ++RL R SG+ GL+ M              + +++  GI+L RPL++ 
Sbjct: 139 TAHTRDDQAETLLMRLVRGSGLAGLSAM--------------ARLTERDGIVLARPLLDV 198

Query: 255 SKDDLYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDMSS 296
            K  L    + A   + +DPTNR + F R R+R  L  +++
Sbjct: 199 PKAQLIATLKRAKIGFADDPTNRDTAFTRPRLRALLPQLAA 214

BLAST of CmaCh11G006120 vs. ExPASy Swiss-Prot
Match: C0R4S1 (tRNA(Ile)-lysidine synthase OS=Wolbachia sp. subsp. Drosophila simulans (strain wRi) OX=66084 GN=tilS PE=3 SV=1)

HSP 1 Score: 123.2 bits (308), Expect = 1.2e-26
Identity = 97/328 (29.57%), Postives = 161/328 (49.09%), Query Frame = 0

Query: 80  HHRIALGVSGGPDSMALCVLMAGW-KTDSLNGTGKSSTFIGGLLAIVVDHGLRAESRDEA 139
           ++++A+ VSGG DS+ L  LM  W K + L+             A+ V+HGLR ES+ EA
Sbjct: 18  NNQVAVAVSGGVDSIVLLHLMTNWAKKNKLSLP----------TALTVNHGLRPESQKEA 77

Query: 140 NVVRNRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQMLQKVCVQHKIGVILVAHH 199
           + V +   ++G + E    +W + +  +G++Q  AR  RY++L + C  + +  +LVAHH
Sbjct: 78  DFVVSYAKELGAK-ESFILNW-EKQNIKGNIQLQARKARYKLLAEWCKNNNVKYLLVAHH 137

Query: 200 ADDQAELFILRLSRSSGVLGLAGMPFTSQIFSPHRYSYSEVSKNYGILLVRPLMEFSKDD 259
            DDQAE F+LRL R SGV GL+ M + S +               GI + RPL+ FS+ +
Sbjct: 138 KDDQAETFLLRLERGSGVDGLSSMDYKSFL--------------NGIYIFRPLLNFSRSE 197

Query: 260 LYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDMSSCTLKSELHAIISACRKTRLFVDH 319
           + K  +     W+ED +N    + R   R  L    +  + +E   +      T L +  
Sbjct: 198 IEKYAKLHRLKWIEDRSNYDLKYRRTLYRNLLKASDNQEILTERICL------TALHMKR 257

Query: 320 ACLSLANLT-------LTITELGYAIINLEILNQLKIQDICLSKFLSLVLQFISQRHRPV 379
           A  +L + T       + + +LGY  I L    QL  ++I L   L  ++   S+ ++P 
Sbjct: 258 AAKALMHYTRLAFNDCVNVHDLGYIEIKLSEFYQLP-EEIALRLLLYSIMAIASKHYKP- 311

Query: 380 RGNTAKLVLDYIQTVPCRTSLTAAGCYI 400
           R N+  ++ + I       + T +GC I
Sbjct: 318 RYNSLIVIFNKILQKDSNVNCTLSGCKI 311

BLAST of CmaCh11G006120 vs. ExPASy Swiss-Prot
Match: Q73FR9 (tRNA(Ile)-lysidine synthase OS=Wolbachia pipientis wMel OX=163164 GN=tilS PE=3 SV=1)

HSP 1 Score: 121.3 bits (303), Expect = 4.7e-26
Identity = 96/328 (29.27%), Postives = 161/328 (49.09%), Query Frame = 0

Query: 80  HHRIALGVSGGPDSMALCVLMAGW-KTDSLNGTGKSSTFIGGLLAIVVDHGLRAESRDEA 139
           ++++A+ VSGG DS+ L  LM  W K + L+            +A+ V+HGLR ES+ EA
Sbjct: 18  NNQVAVAVSGGVDSIVLLHLMTNWAKKNKLSLP----------IALTVNHGLRPESQKEA 77

Query: 140 NVVRNRVTQMGIRCEIASCDWLDGKPKQGHLQEAARDMRYQMLQKVCVQHKIGVILVAHH 199
           + V +   ++G + E    +W + +  +G++Q  AR  RY++L + C  + +  +LVAHH
Sbjct: 78  DFVVSYAKELGAK-ESFILNW-EKQNIKGNIQLQARKARYKLLAEWCKNNNVKYLLVAHH 137

Query: 200 ADDQAELFILRLSRSSGVLGLAGMPFTSQIFSPHRYSYSEVSKNYGILLVRPLMEFSKDD 259
            DDQAE F+LRL R SGV GL+ M + S +               GI + RPL+ FS+ +
Sbjct: 138 KDDQAETFLLRLERGSGVDGLSSMDYKSFL--------------NGIDIFRPLLNFSRSE 197

Query: 260 LYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDMSSCTLKSELHAIISACRKTRLFVDH 319
           + K  +     W+ED +N    + R   R  L    +  + +E   +      T L +  
Sbjct: 198 IEKYAKLHRLKWIEDRSNHDLKYRRTLYRNLLKASDNQEILTERICL------TALHMKR 257

Query: 320 ACLSLANLT-------LTITELGYAIINLEILNQLKIQDICLSKFLSLVLQFISQRHRPV 379
           A  +L + T       + + +LGY  I L    QL  ++I L   L  ++   S+ ++P 
Sbjct: 258 AAKALMHYTRLAFNDCVNVHDLGYIEIKLSEFYQLP-EEIALRLLLYSIMAIASKHYKP- 311

Query: 380 RGNTAKLVLDYIQTVPCRTSLTAAGCYI 400
           R ++   + + I       + T +GC I
Sbjct: 318 RYSSLIAIFNRILQKDSNVNCTLSGCKI 311

BLAST of CmaCh11G006120 vs. TAIR 10
Match: AT3G24560.2 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 682.6 bits (1760), Expect = 3.8e-196
Identity = 364/651 (55.91%), Postives = 455/651 (69.89%), Query Frame = 0

Query: 47  RCFCRYSSLQEPIDMCKYREAFSKRMDMAGLKPHHRIALGVSGGPDSMALCVLMAGWKTD 106
           R FC ++ + E +D  +Y+E F+KRMDMAGLKPH+RIALGVSGGPDSMALCVL A WKT+
Sbjct: 51  RLFCNHACVPETVDETRYKELFNKRMDMAGLKPHNRIALGVSGGPDSMALCVLTAKWKTE 110

Query: 107 SLNGTGKSSTFIGGLLAIVVDHGLRAESRDEANVVRNRVTQMGIRCEIASCDWLDGKPKQ 166
            L+   K+  FI GL+AIVVDHGLR ES+DEA +V +RV+QMGIRCEIASCDW+DG+PK 
Sbjct: 111 GLSCVNKTDGFIDGLVAIVVDHGLRQESKDEAELVCSRVSQMGIRCEIASCDWVDGRPKL 170

Query: 167 GHLQEAARDMRYQMLQKVCVQHKIGVILVAHHADDQAELFILRLSRSSGVLGLAGMPFTS 226
           GHLQEAAR+MRY+M+  VC + +I V+L+AHHADDQAELFILRLSRSSGVLGLAG  F S
Sbjct: 171 GHLQEAAREMRYEMISNVCFRQQIEVLLIAHHADDQAELFILRLSRSSGVLGLAGTAFAS 230

Query: 227 QIFSPHRYSYSEVSKNYGILLVRPLMEFSKDDLYKICQGANQDWVEDPTNRSSLFARNRI 286
           +IFS +    ++  KN  I LVRPL++  K+D+YKICQ   QDWVEDPTNRS LF RNRI
Sbjct: 231 EIFSRNLQLDAKHMKNQSIRLVRPLLDLWKEDMYKICQWGRQDWVEDPTNRSQLFVRNRI 290

Query: 287 RMSLGDMSSCTLKSELHAIISACRKTRLFVDHACLSLANLTLTITELGYAIINLEILNQL 346
           R S+G++ S + KSEL A+IS CR+TR FVD  C  L + T+T+T+ GYAI++LE LN  
Sbjct: 291 RTSIGNLESGSFKSELQAVISECRRTRSFVDKVCTDLIHQTVTVTDKGYAILDLERLNPS 350

Query: 347 KIQDICLSKFLSLVLQFISQRHRPVRGNTAKLVLDYIQTVPCRTSLTAAGCYICPAPGSK 406
            ++DICLSK+L  VLQFISQR RP+RGNT+KL+L+YI+ +PCRTSLTAAGCY+ PAPGSK
Sbjct: 351 GVKDICLSKYLFAVLQFISQRQRPIRGNTSKLLLNYIRAIPCRTSLTAAGCYLSPAPGSK 410

Query: 407 GTRCIVSCSVDCPLPSAVEVFHVPSYKEERHAISGGLEQIINDGKSYLEHFIPKA-SDVH 466
           GT+ IVSCSVDCPLPS  E+ ++ S+ E     S  L QII D KS+ +H  P +  +V 
Sbjct: 411 GTKIIVSCSVDCPLPSKTELLNI-SFNE---TPSDDLGQIIADAKSFSDHVAPTSLFEVQ 470

Query: 467 FLDTTPESVLDEAKRLNILGESTYRIIVSLQMQETQLF--KAEPKVTPGYETKHGSHSVS 526
           FLD   ESVL +A+ LN+L ESTY  I  LQ  ET+ F  K E K     E +HG++  +
Sbjct: 471 FLDVASESVLSKARELNLLSESTYTTIGLLQRDETKRFLTKTEEK---SVELEHGTNIAA 530

Query: 527 TLSSGLLKWGQICNYMNRFFITWKVKDENEDNSISDAASCNTI-CKCCALGCDMELEVRH 586
           +     L  GQ   +MNRF I W + D            CN   C+ C +     +EVRH
Sbjct: 531 SSDKVHLCLGQNLYFMNRFLIRWNLSDH----------QCNEADCRNCPVSTATSMEVRH 590

Query: 587 MTESNWLYLSKLSEGTVENFHDQRDTSASSKEPTMENASRCLKYASSSARKALVLLKLIP 646
           M E +WLYL++LS+ +  N                        ++ SS++KAL  LKLIP
Sbjct: 591 MVEPDWLYLAELSKCSTSN------------------------HSISSSQKALRSLKLIP 650

Query: 647 VAARKSLPVLVNQKGLLLSIPSIKFSHCPWLMASAEFKPRVPFGGGHSSFI 694
            AARKSLPVLVN  GLLL IP+I FS+C  L ASA F PR+P GGGHSSF+
Sbjct: 651 AAARKSLPVLVNHCGLLLCIPAIGFSYCSCLEASAVFLPRIPLGGGHSSFL 660

BLAST of CmaCh11G006120 vs. TAIR 10
Match: AT3G24560.1 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 459.1 bits (1180), Expect = 6.8e-129
Identity = 260/496 (52.42%), Postives = 326/496 (65.73%), Query Frame = 0

Query: 202 QAELFILRLSRSSGVLGLAGMPFTSQIFSPHRYSYSEVSKNYGILLVRPLMEFSKDDLYK 261
           QAELFILRLSRSSGVLGLAG  F S+IFS +    ++  KN  I LVRPL++  K+D+YK
Sbjct: 8   QAELFILRLSRSSGVLGLAGTAFASEIFSRNLQLDAKHMKNQSIRLVRPLLDLWKEDMYK 67

Query: 262 ICQGANQDWVEDPTNRSSLFARNRIRMSLGDMSSCTLKSELHAIISACRKTRLFVDHACL 321
           ICQ   QDWVEDPTNRS LF RNRIR S+G++ S + KSEL A+IS CR+TR FVD  C 
Sbjct: 68  ICQWGRQDWVEDPTNRSQLFVRNRIRTSIGNLESGSFKSELQAVISECRRTRSFVDKVCT 127

Query: 322 SLANLTLTITELGYAIINLEILNQLKIQDICLSKFLSLVLQFISQRHRPVRGNTAKLVLD 381
            L + T+T+T+ GYAI++LE LN   ++DICLSK+L  VLQFISQR RP+RGNT+KL+L+
Sbjct: 128 DLIHQTVTVTDKGYAILDLERLNPSGVKDICLSKYLFAVLQFISQRQRPIRGNTSKLLLN 187

Query: 382 YIQTVPCRTSLTAAGCYICPAPGSKGTRCIVSCSVDCPLPSAVEVFHVPSYKEERHAISG 441
           YI+ +PCRTSLTAAGCY+ PAPGSKGT+ IVSCSVDCPLPS  E+ ++ S+ E     S 
Sbjct: 188 YIRAIPCRTSLTAAGCYLSPAPGSKGTKIIVSCSVDCPLPSKTELLNI-SFNE---TPSD 247

Query: 442 GLEQIINDGKSYLEHFIPKA-SDVHFLDTTPESVLDEAKRLNILGESTYRIIVSLQMQET 501
            L QII D KS+ +H  P +  +V FLD   ESVL +A+ LN+L ESTY  I  LQ  ET
Sbjct: 248 DLGQIIADAKSFSDHVAPTSLFEVQFLDVASESVLSKARELNLLSESTYTTIGLLQRDET 307

Query: 502 QLF--KAEPKVTPGYETKHGSHSVSTLSSGLLKWGQICNYMNRFFITWKVKDENEDNSIS 561
           + F  K E K     E +HG++  ++     L  GQ   +MNRF I W + D        
Sbjct: 308 KRFLTKTEEK---SVELEHGTNIAASSDKVHLCLGQNLYFMNRFLIRWNLSDH------- 367

Query: 562 DAASCNTI-CKCCALGCDMELEVRHMTESNWLYLSKLSEGTVENFHDQRDTSASSKEPTM 621
               CN   C+ C +     +EVRHM E +WLYL++LS+ +  N                
Sbjct: 368 ---QCNEADCRNCPVSTATSMEVRHMVEPDWLYLAELSKCSTSN---------------- 427

Query: 622 ENASRCLKYASSSARKALVLLKLIPVAARKSLPVLVNQKGLLLSIPSIKFSHCPWLMASA 681
                   ++ SS++KAL  LKLIP AARKSLPVLVN  GLLL IP+I FS+C  L ASA
Sbjct: 428 --------HSISSSQKALRSLKLIPAAARKSLPVLVNHCGLLLCIPAIGFSYCSCLEASA 462

Query: 682 EFKPRVPFGGGHSSFI 694
            F PR+P GGGHSSF+
Sbjct: 488 VFLPRIPLGGGHSSFL 462

BLAST of CmaCh11G006120 vs. TAIR 10
Match: AT3G24560.3 (Adenine nucleotide alpha hydrolases-like superfamily protein )

HSP 1 Score: 398.3 bits (1022), Expect = 1.4e-110
Identity = 237/486 (48.77%), Postives = 299/486 (61.52%), Query Frame = 0

Query: 241 KNYGILLVRPLMEFSKDDLYKICQGANQDWVEDPTNRSSLFARNRIRMSLGDMSSCTLKS 300
           KN  I LVRPL++  K+D+YKICQ   QDWVEDPTNRS LF RNRIR S+G++ S + KS
Sbjct: 2   KNQSIRLVRPLLDLWKEDMYKICQWGRQDWVEDPTNRSQLFVRNRIRTSIGNLESGSFKS 61

Query: 301 ELHAIISACRKTRLFVDHACLSLANLTLTIT-ELGYAIINLEILNQLKIQDICLSKFL-- 360
           EL A+IS CR+TR FVD  C  L + T+T+T + GYAI++LE LN   ++DICLSK+L  
Sbjct: 62  ELQAVISECRRTRSFVDKVCTDLIHQTVTVTDQKGYAILDLERLNPSGVKDICLSKYLFA 121

Query: 361 ----------SLV----------------LQFISQRHRPVRGNTAKLVLDYIQTVPCRTS 420
                     SLV                LQFISQR RP+RGNT+KL+L+YI+ +PCRTS
Sbjct: 122 VLQVLHLEFFSLVKLLPFECFLDYSLLSNLQFISQRQRPIRGNTSKLLLNYIRAIPCRTS 181

Query: 421 LTAAGCYICPAPGSKGTRCIVSCSVDCPLPSAVEVFHVPSYKEERHAISGGLEQIINDGK 480
           LTAAGCY+ PAPGSKGT+ IVSCSVDCPLPS  E+ ++ S+ E     S  L QII D K
Sbjct: 182 LTAAGCYLSPAPGSKGTKIIVSCSVDCPLPSKTELLNI-SFNE---TPSDDLGQIIADAK 241

Query: 481 SYLEHFIPKA-SDVHFLDTTPESVLDEAKRLNILGESTYRIIVSLQMQETQLF--KAEPK 540
           S+ +H  P +  +V FLD   ESVL +A+ LN+L ESTY  I  LQ  ET+ F  K E K
Sbjct: 242 SFSDHVAPTSLFEVQFLDVASESVLSKARELNLLSESTYTTIGLLQRDETKRFLTKTEEK 301

Query: 541 VTPGYETKHGSHSVSTLSSGLLKWGQICNYMNRFFITWKVKDENEDNSISDAASCNTI-C 600
                E +HG++  ++     L  GQ   +MNRF I W + D            CN   C
Sbjct: 302 ---SVELEHGTNIAASSDKVHLCLGQNLYFMNRFLIRWNLSDH----------QCNEADC 361

Query: 601 KCCALGCDMELEVRHMTESNWLYLSKLSEGTVENFHDQRDTSASSKEPTMENASRCLKYA 660
           + C +     +EVRHM E +WLYL++LS+ +  N                        ++
Sbjct: 362 RNCPVSTATSMEVRHMVEPDWLYLAELSKCSTSN------------------------HS 421

Query: 661 SSSARKALVLLKLIPVAARKSLPVLVNQKGLLLSIPSIKFSHCPWLMASAEFKPRVPFGG 694
            SS++KAL  LKLIP AARKSLPVLVN  GLLL IP+I FS+C  L ASA F PR+P GG
Sbjct: 422 ISSSQKALRSLKLIPAAARKSLPVLVNHCGLLLCIPAIGFSYCSCLEASAVFLPRIPLGG 446