CmaCh11G005220 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh11G005220
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionBifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein
LocationCma_Chr11: 2505328 .. 2505948 (+)
RNA-Seq ExpressionCmaCh11G005220
SyntenyCmaCh11G005220
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGAGTCCTCAAAAATTTACTCCCTCTTCCTACTTTGCATGCTTTTCCTCTCCACCGCCACACCCATTTTCGGCTGCGGCGCCTGCGGCAACCCGCCACCCAAAAAGGTCAAACCCGGCTCCGGTTCCAAGTCCCCTAAAGGAGGCCCCATTGGCCTTCCACCCATTTCCCTCCCCCCCATCGTTAAACCGCCCATCGACCTCCCTCCCGTCGTAAAACCGCCAATTTCCCTCCCCCCCATCGTCAAACCGCCTGTCGACCTCCCACCCCTCCCTCCCGTTACCCTTCCTCCAGTCACCGTCCCTCCAGTTCTCACAAAGCCATCTCCGGCGTCGAAGCCCTGCCCCCCTTCAGGGAAAGAGACCTGCCCAATTGACACTCTGAAATTGGGCGCATGTGTGGATCTTCTGGGCGGGCTGGTGCACATTGGGCTAGGAGACCCTGCAGTGAACGAATGCTGCCCGGTACTCTCAGGGCTGGCTGAGGTGGAAGCGGCGGTTTGCCTTTGCACTACCCTTAAAATTAAGGCTCTTAGCCTTAACATCTATGTTCCCATTGCCCTTCAGCTCCTTATCAGCTGTGGGAAGACCCCTCCCCCTGGCTACACTTGCTCCCTTTAG

mRNA sequence

ATGGAGTCCTCAAAAATTTACTCCCTCTTCCTACTTTGCATGCTTTTCCTCTCCACCGCCACACCCATTTTCGGCTGCGGCGCCTGCGGCAACCCGCCACCCAAAAAGGTCAAACCCGGCTCCGGTTCCAAGTCCCCTAAAGGAGGCCCCATTGGCCTTCCACCCATTTCCCTCCCCCCCATCGTTAAACCGCCCATCGACCTCCCTCCCGTCGTAAAACCGCCAATTTCCCTCCCCCCCATCGTCAAACCGCCTGTCGACCTCCCACCCCTCCCTCCCGTTACCCTTCCTCCAGTCACCGTCCCTCCAGTTCTCACAAAGCCATCTCCGGCGTCGAAGCCCTGCCCCCCTTCAGGGAAAGAGACCTGCCCAATTGACACTCTGAAATTGGGCGCATGTGTGGATCTTCTGGGCGGGCTGGTGCACATTGGGCTAGGAGACCCTGCAGTGAACGAATGCTGCCCGGTACTCTCAGGGCTGGCTGAGGTGGAAGCGGCGGTTTGCCTTTGCACTACCCTTAAAATTAAGGCTCTTAGCCTTAACATCTATGTTCCCATTGCCCTTCAGCTCCTTATCAGCTGTGGGAAGACCCCTCCCCCTGGCTACACTTGCTCCCTTTAG

Coding sequence (CDS)

ATGGAGTCCTCAAAAATTTACTCCCTCTTCCTACTTTGCATGCTTTTCCTCTCCACCGCCACACCCATTTTCGGCTGCGGCGCCTGCGGCAACCCGCCACCCAAAAAGGTCAAACCCGGCTCCGGTTCCAAGTCCCCTAAAGGAGGCCCCATTGGCCTTCCACCCATTTCCCTCCCCCCCATCGTTAAACCGCCCATCGACCTCCCTCCCGTCGTAAAACCGCCAATTTCCCTCCCCCCCATCGTCAAACCGCCTGTCGACCTCCCACCCCTCCCTCCCGTTACCCTTCCTCCAGTCACCGTCCCTCCAGTTCTCACAAAGCCATCTCCGGCGTCGAAGCCCTGCCCCCCTTCAGGGAAAGAGACCTGCCCAATTGACACTCTGAAATTGGGCGCATGTGTGGATCTTCTGGGCGGGCTGGTGCACATTGGGCTAGGAGACCCTGCAGTGAACGAATGCTGCCCGGTACTCTCAGGGCTGGCTGAGGTGGAAGCGGCGGTTTGCCTTTGCACTACCCTTAAAATTAAGGCTCTTAGCCTTAACATCTATGTTCCCATTGCCCTTCAGCTCCTTATCAGCTGTGGGAAGACCCCTCCCCCTGGCTACACTTGCTCCCTTTAG

Protein sequence

MESSKIYSLFLLCMLFLSTATPIFGCGACGNPPPKKVKPGSGSKSPKGGPIGLPPISLPPIVKPPIDLPPVVKPPISLPPIVKPPVDLPPLPPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLISCGKTPPPGYTCSL
Homology
BLAST of CmaCh11G005220 vs. ExPASy Swiss-Prot
Match: Q00451 (36.4 kDa proline-rich protein OS=Solanum lycopersicum OX=4081 GN=TPRP-F1 PE=2 SV=1)

HSP 1 Score: 139.4 bits (350), Expect = 4.5e-32
Identity = 98/226 (43.36%), Postives = 121/226 (53.54%), Query Frame = 0

Query: 33  PPKKVKPGSGSKSPKGGPI-GLPPISLPPIVKPPIDLPPVV------------KPPISLP 92
           PP  V P S  K PK  P    PP  +PPIV PPI  PP+             KPP   P
Sbjct: 118 PPPIVSPPSTPKPPKTPPFTPKPPSPIPPIVSPPIVYPPITPTPPIVHPPVTPKPPSPTP 177

Query: 93  PIVKPPVDLPPL-------------------------PPVTLPP--------VTVPPVLT 152
           PIV PP+  PP+                         PPV +PP        VT P V T
Sbjct: 178 PIVSPPIVYPPITPTPPVVSPPIIPTPPIVSPPFVPNPPVVIPPPYVPSPPVVTPPIVPT 237

Query: 153 KPSPASKPCP-------PSGKETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSG 205
            P+P   P P       P  + TCPID LKLGACVD+LGGL+HIG+G  A   CCP+L G
Sbjct: 238 PPTPCPPPPPPPAIIPSPPAQPTCPIDALKLGACVDVLGGLIHIGIGGSAKQTCCPLLGG 297

BLAST of CmaCh11G005220 vs. ExPASy Swiss-Prot
Match: Q9SU35 (pEARLI1-like lipid transfer protein 1 OS=Arabidopsis thaliana OX=3702 GN=AZI1 PE=1 SV=1)

HSP 1 Score: 89.7 bits (221), Expect = 4.1e-17
Identity = 56/133 (42.11%), Postives = 80/133 (60.15%), Query Frame = 0

Query: 80  PIVKP-PVDLPPLPPVTLPPVTVPPVLTKPSPASKPCPP-----SGKETCPIDTLKLGAC 139
           P  KP PV  P   PV  PP   PP  + PSP  +P  P     S   +CPID LKLG C
Sbjct: 32  PSPKPKPVPSPKPKPVQCPP---PPRPSVPSPNPRPVTPPRTPGSSGNSCPIDALKLGVC 91

Query: 140 VDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLIS 199
            ++L  L++I LG P+  +CC ++ GL +V+AA+CLCT L+   L +N+ VPI+L +L++
Sbjct: 92  ANVLSSLLNIQLGQPSSQQCCSLIQGLVDVDAAICLCTALRANVLGINLNVPISLSVLLN 151

Query: 200 -CGKTPPPGYTCS 206
            C +  P G+ C+
Sbjct: 152 VCNRKLPSGFQCA 161

BLAST of CmaCh11G005220 vs. ExPASy Swiss-Prot
Match: Q39176 (Lipid transfer protein EARLI 1 OS=Arabidopsis thaliana OX=3702 GN=EARLI1 PE=1 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.6e-16
Identity = 52/129 (40.31%), Postives = 76/129 (58.91%), Query Frame = 0

Query: 80  PIVKP-PVDLPPLPPVTLPPVTVPPVLT-KPSPASKPCPPSGKETCPIDTLKLGACVDLL 139
           P  KP PV  P   PV  P V  P V +  P P + P  P     CPID L+LG C ++L
Sbjct: 40  PSPKPKPVPSPKPKPVPSPSVPSPSVPSPNPRPVTPPRTPGSSGNCPIDALRLGVCANVL 99

Query: 140 GGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLIS-CGK 199
             L++I LG P+   CC ++ GL +++AA+CLCT L+   L +N+ VPI+L +L++ C +
Sbjct: 100 SSLLNIQLGQPSAQPCCSLIQGLVDLDAAICLCTALRANVLGINLNVPISLSVLLNVCNR 159

Query: 200 TPPPGYTCS 206
             P G+ C+
Sbjct: 160 KVPSGFQCA 168

BLAST of CmaCh11G005220 vs. ExPASy Swiss-Prot
Match: Q9SU34 (pEARLI1-like lipid transfer protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g12490 PE=2 SV=1)

HSP 1 Score: 87.0 bits (214), Expect = 2.6e-16
Identity = 53/147 (36.05%), Postives = 84/147 (57.14%), Query Frame = 0

Query: 60  PIVKPPIDLPPVVKPPISLPPIVKPPVDLPPLPPVTLPPVTVPPVLTKPSPASKPCPPSG 119
           P+  P +  P V  P +  P +  P V  P +P  ++P  +VP     P+P   P  P  
Sbjct: 38  PLPNPKVPSPKVPTPSVPSPYVPTPSVPSPSVPTPSVPSPSVPS--PNPTPVIPPRTPGS 97

Query: 120 KETCPIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALS 179
              CPID L+LG C ++L GL+++ LG P+   CC ++ GL +++AAVCLCT L+   L 
Sbjct: 98  SGNCPIDALRLGVCANVLSGLLNVQLGQPSPQPCCSLIQGLVDLDAAVCLCTALRANVLG 157

Query: 180 LNIYVPIALQLLIS-CGKTPPPGYTCS 206
           +N+ VPI+L +L++ C +  P  + C+
Sbjct: 158 INLNVPISLSVLLNVCNRRLPSNFQCA 182

BLAST of CmaCh11G005220 vs. ExPASy Swiss-Prot
Match: Q9SU33 (pEARLI1-like lipid transfer protein 3 OS=Arabidopsis thaliana OX=3702 GN=At4g12500 PE=2 SV=1)

HSP 1 Score: 86.7 bits (213), Expect = 3.4e-16
Identity = 51/138 (36.96%), Postives = 80/138 (57.97%), Query Frame = 0

Query: 69  PPVVKPPISLPPIVKPPVDLPPLPPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTL 128
           P V  P    P I  P V  P +P  ++P  +VP     P+P + P  P     CPID L
Sbjct: 42  PKVPSPKYPSPSIPSPSVPTPSVPTPSVPTPSVPS--PNPTPVTPPRTPGSSGNCPIDAL 101

Query: 129 KLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIAL 188
           +LG C ++L GL+++ LG P+   CC ++ GL +++AA+CLCT L+   L +N+ VPI+L
Sbjct: 102 RLGVCANVLSGLLNVQLGQPSAQPCCSLIQGLVDLDAAICLCTALRANVLGINLNVPISL 161

Query: 189 QLLIS-CGKTPPPGYTCS 206
            +L++ C +  P  + C+
Sbjct: 162 SVLLNVCNRRLPSDFQCA 177

BLAST of CmaCh11G005220 vs. TAIR 10
Match: AT2G10940.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.1e-44
Identity = 104/199 (52.26%), Postives = 129/199 (64.82%), Query Frame = 0

Query: 33  PPKKVKPGSGSKSPKGGPIGLPPISLPPIVKPPIDLPPVVKPPISLPPIVKPPVDLPPLP 92
           P   V P +  K P   P+ +P + +PP+  P + +PPV  P + +PP+  P + LPP+ 
Sbjct: 94  PKLPVPPVTVPKLPV-PPVTVPKLPVPPVTVPKLPVPPVTVPKLPVPPVTVPKLPLPPIS 153

Query: 93  PVTLPPVT---------------VPPVLTKPSPASKPCPP----------SGKETCPIDT 152
            + +PPV                +PP  T P+   K CPP           GK TCPIDT
Sbjct: 154 GLPIPPVVGPNLPLPPLPIVGPILPPGTTPPATGGKDCPPPPGSVKPPSGGGKATCPIDT 213

Query: 153 LKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIA 207
           LKLGACVDLLGGLV IGLGDPAVN+CCP+L GL EVEAA CLCTTLK+KAL LN+YVP+A
Sbjct: 214 LKLGACVDLLGGLVKIGLGDPAVNKCCPLLKGLVEVEAAACLCTTLKLKALDLNLYVPVA 273

BLAST of CmaCh11G005220 vs. TAIR 10
Match: AT2G10940.2 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 177.6 bits (449), Expect = 1.1e-44
Identity = 104/199 (52.26%), Postives = 129/199 (64.82%), Query Frame = 0

Query: 33  PPKKVKPGSGSKSPKGGPIGLPPISLPPIVKPPIDLPPVVKPPISLPPIVKPPVDLPPLP 92
           P   V P +  K P   P+ +P + +PP+  P + +PPV  P + +PP+  P + LPP+ 
Sbjct: 94  PKLPVPPVTVPKLPV-PPVTVPKLPVPPVTVPKLPVPPVTVPKLPVPPVTVPKLPLPPIS 153

Query: 93  PVTLPPVT---------------VPPVLTKPSPASKPCPP----------SGKETCPIDT 152
            + +PPV                +PP  T P+   K CPP           GK TCPIDT
Sbjct: 154 GLPIPPVVGPNLPLPPLPIVGPILPPGTTPPATGGKDCPPPPGSVKPPSGGGKATCPIDT 213

Query: 153 LKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIA 207
           LKLGACVDLLGGLV IGLGDPAVN+CCP+L GL EVEAA CLCTTLK+KAL LN+YVP+A
Sbjct: 214 LKLGACVDLLGGLVKIGLGDPAVNKCCPLLKGLVEVEAAACLCTTLKLKALDLNLYVPVA 273

BLAST of CmaCh11G005220 vs. TAIR 10
Match: AT1G62500.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 162.5 bits (410), Expect = 3.5e-40
Identity = 100/201 (49.75%), Postives = 127/201 (63.18%), Query Frame = 0

Query: 32  PPPKKVKPGSGSKSP---------KGGPIGLPPISLPPIVKPPIDLPPVVKPPISLPPIV 91
           PPP  V+P    + P         +  PI  PPI +PPI  PP+  PP + PPI+ PP +
Sbjct: 94  PPPVVVRPPPIIRPPPVVYPPPIVRPPPITRPPIIIPPIQPPPVTTPPGLLPPITTPPGL 153

Query: 92  KPPVDLPP--LPPVTLPPVTVPPVLTKPSPASKPCPPSG-----------------KETC 151
            PPV  PP  LPPVT PP  +PP++  P P + P P SG                 + TC
Sbjct: 154 LPPVTTPPGLLPPVTTPPGLLPPII-NPPPVTVPPPSSGYPPYGPPSGGGGGGGGKQPTC 213

Query: 152 PIDTLKLGACVDLLGGLVHIGLGDPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIY 205
           PI+ LKLGACVD+LGGL+HIGLG+P  N CCPVL GL E+EAAVCLCTT+++K L+LNI+
Sbjct: 214 PINALKLGACVDVLGGLIHIGLGNPVENVCCPVLQGLLELEAAVCLCTTIRLKLLNLNIF 273

BLAST of CmaCh11G005220 vs. TAIR 10
Match: AT3G22142.1 (Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamily protein )

HSP 1 Score: 162.2 bits (409), Expect = 4.6e-40
Identity = 89/177 (50.28%), Postives = 122/177 (68.93%), Query Frame = 0

Query: 38   KPGSGSKSPKGGPIGLPPISLPPIVKPPIDLPPVVKPPISLPPIVKPPVDLPPL------ 97
            KP   +  P   P+  PP++ PP V PP+  PP+ KPP + PP+  PPV  PP+      
Sbjct: 1303 KPPVATPPPATPPVAKPPVATPPTVTPPVATPPIAKPPGARPPVATPPVATPPIAKSPVA 1362

Query: 98   -PPVTLPPVTVPPVLTKP---SPASKPCPPSGKETCPIDTLKLGACVDLLGGLVHIGLGD 157
             PP+T PPV   P+ T P   +P + P P +  +TCPIDTLKLG+CVDLLGGLVHIG+G 
Sbjct: 1363 TPPMTKPPVASSPIATPPIAKTPIATP-PTTMPKTCPIDTLKLGSCVDLLGGLVHIGIGK 1422

Query: 158  PAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLISCGKTPPPGYTC 205
             A  +CCPV+ GL +++AAVCLCTT+K K L++++ +PIAL++L++CGK PPPG+ C
Sbjct: 1423 SAKEKCCPVVEGLVDLDAAVCLCTTIKAKLLNIDVILPIALEVLLNCGKNPPPGFKC 1478

BLAST of CmaCh11G005220 vs. TAIR 10
Match: AT3G22120.1 (cell wall-plasma membrane linker protein )

HSP 1 Score: 161.8 bits (408), Expect = 6.0e-40
Identity = 93/181 (51.38%), Postives = 119/181 (65.75%), Query Frame = 0

Query: 32  PPPKKVKPGSGSKSPKGGPIGLPPISLPPIVKPPIDLPPVVKPPISLPPIVKPPVDLPPL 91
           PPP    P + + +P   P+  PP   PP++ PP   PPVV PP   PP++ PP   PP+
Sbjct: 158 PPPTPCPPPTPTPTP---PVVTPPTPTPPVITPPTPTPPVVTPPTPTPPVITPPTPTPPV 217

Query: 92  -------PPVTLPPVTVPPVLTKPSPASKPCPPSGKETCPIDTLKLGACVDLLGGLVHIG 151
                  PPV  PP   PPV+T P+P     P    ETCPIDTLKLGACVD+LGGL+HIG
Sbjct: 218 ITPPTPTPPVVTPPTPTPPVVTPPTPTP---PTPIPETCPIDTLKLGACVDVLGGLIHIG 277

Query: 152 LG-DPAVNECCPVLSGLAEVEAAVCLCTTLKIKALSLNIYVPIALQLLISCGKTPPPGYT 205
           LG   A  ECCPVL GL +++AAVCLCTT+K+K L++++ +PIAL+LL+ CGKTPP  + 
Sbjct: 278 LGKSHAKAECCPVLGGLLDLDAAVCLCTTIKLKLLNIDLVLPIALELLLDCGKTPPSDFK 332

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q004514.5e-3243.3636.4 kDa proline-rich protein OS=Solanum lycopersicum OX=4081 GN=TPRP-F1 PE=2 SV... [more]
Q9SU354.1e-1742.11pEARLI1-like lipid transfer protein 1 OS=Arabidopsis thaliana OX=3702 GN=AZI1 PE... [more]
Q391762.6e-1640.31Lipid transfer protein EARLI 1 OS=Arabidopsis thaliana OX=3702 GN=EARLI1 PE=1 SV... [more]
Q9SU342.6e-1636.05pEARLI1-like lipid transfer protein 2 OS=Arabidopsis thaliana OX=3702 GN=At4g124... [more]
Q9SU333.4e-1636.96pEARLI1-like lipid transfer protein 3 OS=Arabidopsis thaliana OX=3702 GN=At4g125... [more]
Match NameE-valueIdentityDescription
AT2G10940.11.1e-4452.26Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT2G10940.21.1e-4452.26Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT1G62500.13.5e-4049.75Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT3G22142.14.6e-4050.28Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumin superfamil... [more]
AT3G22120.16.0e-4051.38cell wall-plasma membrane linker protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR016140Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domainSMARTSM00499aai_6coord: 123..204
e-value: 2.5E-5
score: 33.7
IPR027923Hydrophobic seed protein domainPFAMPF14547Hydrophob_seedcoord: 122..204
e-value: 2.1E-27
score: 95.2
IPR027923Hydrophobic seed protein domainCDDcd01958HPS_likecoord: 122..204
e-value: 5.60383E-26
score: 93.9324
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilyGENE3D1.10.110.10coord: 122..205
e-value: 1.6E-22
score: 81.1
IPR036312Bifunctional inhibitor/plant lipid transfer protein/seed storage helical domain superfamilySUPERFAMILY47699Bifunctional inhibitor/lipid-transfer protein/seed storage 2S albumincoord: 125..205
NoneNo IPR availablePANTHERPTHR31731FAMILY NOT NAMEDcoord: 112..205
NoneNo IPR availablePANTHERPTHR31731:SF136.4 KDA PROLINE-RICH PROTEINcoord: 1..107
coord: 112..205
NoneNo IPR availablePANTHERPTHR31731FAMILY NOT NAMEDcoord: 1..107

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G005220.1CmaCh11G005220.1mRNA