CmaCh11G003130 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh11G003130
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionAuxin efflux carrier component
LocationCma_Chr11: 1558630 .. 1561563 (-)
RNA-Seq ExpressionCmaCh11G003130
SyntenyCmaCh11G003130
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRpolypeptideCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CACTTTTCTCTCTCTCTCTCTAAACTGAAACAAGAAATTAGTTTTAAAAACACACGTGCTTTCCCATTCTTCTTCTTCTTCTTCATCCAACTTCCTCTGTTTTCCTCTGCATTTCATCCTCTGTTTTCCTCTGAATTTGAAGTTACTTAAAGATGATTACATTACTGGACTTCTACCATGTCATGACCGCCGTCGTCCCCCTCTACGTCGCCATGATCTTAGCTTACGGCTCCGTCAAATGGTGGAAGATCTTCACCCCCGATCAGTGCTCCGGTATCAACCGTTTCGTCGCCCTCTTCGCCGTTCCCCTTCTCTCCTTCCACTTCATAGCCTCCAATGATCCTTATACCATGAGCTTTCGCTTCATCGCCGCCGATACTCTCCAGAAAATCATGGTCCTCGTCGTCCTCGCCATCTGGACTAAGGTAATATCAAACTACCACCAATTTTTCCCAATGGGTTACAATGAATTTTAATGTAGAAATTTAAAATTTGAAGTTAATGGGTTTGGGAGAATTTAATTTCCTTGTTCTTTGATTCCTTTTTAACAGGTGAGCCGGCGGGGGTGCTTGGAATGGACAATTACTCTGTTTTCACTTTCAACTCTGCCCAACACTCTGGTTATGGGAATTCCTCTGCTCAAAGGAATGTACGGCGACTTTTCCGGCAGCTTAATGGTTCAAATTGTGGTTCTTCAATGCATAATTTGGTACACTTTAATGCTCTTCATGTTCGAATTCAGAGGAGCTAGGAATTTAATCTCCGAGCAATTTCCCGACACGGCGGCGTCCATTGTCTCCATTCACGTCGAATCCGACGTCGTTTCTCTCGACGGCCAGCAAGTATTGGAAACAGAGGCGGAGATAAAAGACGACGGAAAGCTCCATGTCACTGTCAGACGGTCCAACGCCTCTCGTTCTGATATTTTCTCCCGCCGGTCACAGGGGCTGTCGTCGACGACTCCCCGGCCATCCAATCTCACCAACGCCGAGATTTATTCGTTGCAGTCATCGAGAAATCCAACGCCAAGAGGCTCGAGTTTTAATCACACTGATTTTTACTCCATGCTTGGCACCGGTGGGGTGGCACCAGCCGTGTCGAGAGGGCCGACGCCACGGCCGTCGAATTATGAGGAGGAGAATCACGGCGGAGGAGGGAAAAGTGGGCCGAGATATTATTATCATGGGAATCCAGCGCCGCCGCATTACCCTGCTCCGAACATGGGAATGTTTTCACCGACGGGTTCAAGAAATAAGAGATCAACGGCGAACGGACAGGTCGGCCAGTACAAATCGGAGGATGGTAATAATAATAACAACAATAATAAAGATCTTCATATGTTTGTTTGGAGTTCAAGTGCTTCCCCTGTTTCTGATGTGTTTGGAGGCCATGATTATGGAGGTCCCCATGATCATAAAGAACTTAAATTGGCTGTCTCCCCCGGAAAAGGTTCGTTTTTTTTTCAAAATTTTCTCGGATTCTATACCAATTTTCAATCTCGCATTGGTGGAAAAAGAGGACGAAGCATTGTTTATAAGGGTAGGGTGTGGAAACCTCCTCCTAACAGACATGTTTTAGTTCAAAAAAGTCAATATTTGCTAGCAGTAGACATCAGTTGAGTAATGATTTAACGGGTCAGATGTTACAGGCGAGAGTGGGCCGAGACATAACGATAGAGAAGACGTGGAGCGAGAGGAGTTTAGCTTTGGAAACAGAGGGATGAACAGCAGCAACAATGGACATGAAATGGCGGCGGGAGAGAAAGGAGGGACCAGTGGAGTGTCAAAGACAATGCCACCGGCCAGTGTGATGACAAGGCTGATTCTTATAATGGTTTGGAGAAAGCTGATCAGAAACCCCAACACTTACTCTAGCTTGATTGGCCTCACTTGGTCCCTTGTTTCATTCAGGTACTTTTTTCATTAAAAACATCAAAACTTTGGGCTTGGTTGATGTGTTTTTTTGTTGTCTTTGTTAGGTGGCATGTTGAGATGCCTGCCATTGTAGCACAGTCCATCTCGATACTCTCCGACGCCGGGCTCGGCATGGCTATGTTTAGTCTCGGTACGGGATTCTTAAAACGTTCTACACTATATACAAGCGTTAACTCTGAATGATTCGAAAAAAGTTGGATTTTGTTAGGTTTGTTCATGGCTTTGCAACCAAGGATCATAGCATGTGGAAACTCCATAGCAACTTTTGCCATGGCTGTTAGATTCCTTGTAGGCCCAGCGGTAATGGCAGCTGCTTCCATTGCTGTTGGCCTCAAGGGCGTTCTCTTGCATGTTGCCATTGTTCAGGTCCTCTTTCGTTTCTCGTATTTCTACATTAACGTGATATCGCCAGTTTTAACTCTAAACCCGTCGCATAAATTTTCTTTTGAAACAGGCAGCTCTTCCTCAAGGGATTGTACCGTTTGTGTTTGCTAAAGAGTATAACGTACACCCAGATATTTTGAGCACTGCGTAAGTTCTATATCTGGAAATGAGATCAGTTCAAACTAAGACCATTTCGATTAACGTTTATAATCATTGTGTTGCAGAGTGATATTCGGGATGTTGATTGCACTACCGATAACGTTGGTGTACTACATTTTGTTGGGAGTTTGAAAGGTTTGTGTTTAAACACGAAGGATTGAAGAGTTCGTATATACGAGCAAAACAAATGGAGAAGAATCGGCGAGCATTAGACAAGAAGATAACGAAGATTATTAATCGAAGAATGGAAAATAACTAAGAGATTTGAGATTTTTTTTTTTCATGAAGTTGTATTTAATCATGTTTGTAGCCAAATTGTTTTACATAAACTTTTCTTTCCTTTTGTTTCCTTCTTTTTCGTCGTTGTCTTCTTGTTTTTTTCCGTTCAAGAGATCTCGTGTTGCAAATTTGAAAAAGATCGAGGATAACAGAATAAGTTTGTGTCCATCGTGTAAAA

mRNA sequence

CACTTTTCTCTCTCTCTCTCTAAACTGAAACAAGAAATTAGTTTTAAAAACACACGTGCTTTCCCATTCTTCTTCTTCTTCTTCATCCAACTTCCTCTGTTTTCCTCTGCATTTCATCCTCTGTTTTCCTCTGAATTTGAAGTTACTTAAAGATGATTACATTACTGGACTTCTACCATGTCATGACCGCCGTCGTCCCCCTCTACGTCGCCATGATCTTAGCTTACGGCTCCGTCAAATGGTGGAAGATCTTCACCCCCGATCAGTGCTCCGGTATCAACCGTTTCGTCGCCCTCTTCGCCGTTCCCCTTCTCTCCTTCCACTTCATAGCCTCCAATGATCCTTATACCATGAGCTTTCGCTTCATCGCCGCCGATACTCTCCAGAAAATCATGGTCCTCGTCGTCCTCGCCATCTGGACTAAGGTGAGCCGGCGGGGGTGCTTGGAATGGACAATTACTCTGTTTTCACTTTCAACTCTGCCCAACACTCTGGTTATGGGAATTCCTCTGCTCAAAGGAATGTACGGCGACTTTTCCGGCAGCTTAATGGTTCAAATTGTGGTTCTTCAATGCATAATTTGGTACACTTTAATGCTCTTCATGTTCGAATTCAGAGGAGCTAGGAATTTAATCTCCGAGCAATTTCCCGACACGGCGGCGTCCATTGTCTCCATTCACGTCGAATCCGACGTCGTTTCTCTCGACGGCCAGCAAGTATTGGAAACAGAGGCGGAGATAAAAGACGACGGAAAGCTCCATGTCACTGTCAGACGGTCCAACGCCTCTCGTTCTGATATTTTCTCCCGCCGGTCACAGGGGCTGTCGTCGACGACTCCCCGGCCATCCAATCTCACCAACGCCGAGATTTATTCGTTGCAGTCATCGAGAAATCCAACGCCAAGAGGCTCGAGTTTTAATCACACTGATTTTTACTCCATGCTTGGCACCGGTGGGGTGGCACCAGCCGTGTCGAGAGGGCCGACGCCACGGCCGTCGAATTATGAGGAGGAGAATCACGGCGGAGGAGGGAAAAGTGGGCCGAGATATTATTATCATGGGAATCCAGCGCCGCCGCATTACCCTGCTCCGAACATGGGAATGTTTTCACCGACGGGTTCAAGAAATAAGAGATCAACGGCGAACGGACAGGTCGGCCAGTACAAATCGGAGGATGGTAATAATAATAACAACAATAATAAAGATCTTCATATGTTTGTTTGGAGTTCAAGTGCTTCCCCTGTTTCTGATGTGTTTGGAGGCCATGATTATGGAGGTCCCCATGATCATAAAGAACTTAAATTGGCTGTCTCCCCCGGAAAAGGCGAGAGTGGGCCGAGACATAACGATAGAGAAGACGTGGAGCGAGAGGAGTTTAGCTTTGGAAACAGAGGGATGAACAGCAGCAACAATGGACATGAAATGGCGGCGGGAGAGAAAGGAGGGACCAGTGGAGTGTCAAAGACAATGCCACCGGCCAGTGTGATGACAAGGCTGATTCTTATAATGGTTTGGAGAAAGCTGATCAGAAACCCCAACACTTACTCTAGCTTGATTGGCCTCACTTGGTCCCTTGTTTCATTCAGGTGGCATGTTGAGATGCCTGCCATTGTAGCACAGTCCATCTCGATACTCTCCGACGCCGGGCTCGGCATGGCTATGTTTAGTCTCGGTTTGTTCATGGCTTTGCAACCAAGGATCATAGCATGTGGAAACTCCATAGCAACTTTTGCCATGGCTGTTAGATTCCTTGTAGGCCCAGCGGTAATGGCAGCTGCTTCCATTGCTGTTGGCCTCAAGGGCGTTCTCTTGCATGTTGCCATTGTTCAGGCAGCTCTTCCTCAAGGGATTGTACCGTTTGTGTTTGCTAAAGAGTATAACGTACACCCAGATATTTTGAGCACTGCAGTGATATTCGGGATGTTGATTGCACTACCGATAACGTTGGTGTACTACATTTTGTTGGGAGTTTGAAAGGTTTGTGTTTAAACACGAAGGATTGAAGAGTTCGTATATACGAGCAAAACAAATGGAGAAGAATCGGCGAGCATTAGACAAGAAGATAACGAAGATTATTAATCGAAGAATGGAAAATAACTAAGAGATTTGAGATTTTTTTTTTTCATGAAGTTGTATTTAATCATGTTTGTAGCCAAATTGTTTTACATAAACTTTTCTTTCCTTTTGTTTCCTTCTTTTTCGTCGTTGTCTTCTTGTTTTTTTCCGTTCAAGAGATCTCGTGTTGCAAATTTGAAAAAGATCGAGGATAACAGAATAAGTTTGTGTCCATCGTGTAAAA

Coding sequence (CDS)

ATGATTACATTACTGGACTTCTACCATGTCATGACCGCCGTCGTCCCCCTCTACGTCGCCATGATCTTAGCTTACGGCTCCGTCAAATGGTGGAAGATCTTCACCCCCGATCAGTGCTCCGGTATCAACCGTTTCGTCGCCCTCTTCGCCGTTCCCCTTCTCTCCTTCCACTTCATAGCCTCCAATGATCCTTATACCATGAGCTTTCGCTTCATCGCCGCCGATACTCTCCAGAAAATCATGGTCCTCGTCGTCCTCGCCATCTGGACTAAGGTGAGCCGGCGGGGGTGCTTGGAATGGACAATTACTCTGTTTTCACTTTCAACTCTGCCCAACACTCTGGTTATGGGAATTCCTCTGCTCAAAGGAATGTACGGCGACTTTTCCGGCAGCTTAATGGTTCAAATTGTGGTTCTTCAATGCATAATTTGGTACACTTTAATGCTCTTCATGTTCGAATTCAGAGGAGCTAGGAATTTAATCTCCGAGCAATTTCCCGACACGGCGGCGTCCATTGTCTCCATTCACGTCGAATCCGACGTCGTTTCTCTCGACGGCCAGCAAGTATTGGAAACAGAGGCGGAGATAAAAGACGACGGAAAGCTCCATGTCACTGTCAGACGGTCCAACGCCTCTCGTTCTGATATTTTCTCCCGCCGGTCACAGGGGCTGTCGTCGACGACTCCCCGGCCATCCAATCTCACCAACGCCGAGATTTATTCGTTGCAGTCATCGAGAAATCCAACGCCAAGAGGCTCGAGTTTTAATCACACTGATTTTTACTCCATGCTTGGCACCGGTGGGGTGGCACCAGCCGTGTCGAGAGGGCCGACGCCACGGCCGTCGAATTATGAGGAGGAGAATCACGGCGGAGGAGGGAAAAGTGGGCCGAGATATTATTATCATGGGAATCCAGCGCCGCCGCATTACCCTGCTCCGAACATGGGAATGTTTTCACCGACGGGTTCAAGAAATAAGAGATCAACGGCGAACGGACAGGTCGGCCAGTACAAATCGGAGGATGGTAATAATAATAACAACAATAATAAAGATCTTCATATGTTTGTTTGGAGTTCAAGTGCTTCCCCTGTTTCTGATGTGTTTGGAGGCCATGATTATGGAGGTCCCCATGATCATAAAGAACTTAAATTGGCTGTCTCCCCCGGAAAAGGCGAGAGTGGGCCGAGACATAACGATAGAGAAGACGTGGAGCGAGAGGAGTTTAGCTTTGGAAACAGAGGGATGAACAGCAGCAACAATGGACATGAAATGGCGGCGGGAGAGAAAGGAGGGACCAGTGGAGTGTCAAAGACAATGCCACCGGCCAGTGTGATGACAAGGCTGATTCTTATAATGGTTTGGAGAAAGCTGATCAGAAACCCCAACACTTACTCTAGCTTGATTGGCCTCACTTGGTCCCTTGTTTCATTCAGGTGGCATGTTGAGATGCCTGCCATTGTAGCACAGTCCATCTCGATACTCTCCGACGCCGGGCTCGGCATGGCTATGTTTAGTCTCGGTTTGTTCATGGCTTTGCAACCAAGGATCATAGCATGTGGAAACTCCATAGCAACTTTTGCCATGGCTGTTAGATTCCTTGTAGGCCCAGCGGTAATGGCAGCTGCTTCCATTGCTGTTGGCCTCAAGGGCGTTCTCTTGCATGTTGCCATTGTTCAGGCAGCTCTTCCTCAAGGGATTGTACCGTTTGTGTTTGCTAAAGAGTATAACGTACACCCAGATATTTTGAGCACTGCAGTGATATTCGGGATGTTGATTGCACTACCGATAACGTTGGTGTACTACATTTTGTTGGGAGTTTGA

Protein sequence

MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIASNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPLLKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESDVVSLDGQQVLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEIYSLQSSRNPTPRGSSFNHTDFYSMLGTGGVAPAVSRGPTPRPSNYEEENHGGGGKSGPRYYYHGNPAPPHYPAPNMGMFSPTGSRNKRSTANGQVGQYKSEDGNNNNNNNKDLHMFVWSSSASPVSDVFGGHDYGGPHDHKELKLAVSPGKGESGPRHNDREDVEREEFSFGNRGMNSSNNGHEMAAGEKGGTSGVSKTMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWHVEMPAIVAQSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIATFAMAVRFLVGPAVMAAASIAVGLKGVLLHVAIVQAALPQGIVPFVFAKEYNVHPDILSTAVIFGMLIALPITLVYYILLGV
Homology
BLAST of CmaCh11G003130 vs. ExPASy Swiss-Prot
Match: Q9C6B8 (Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV=1)

HSP 1 Score: 802.0 bits (2070), Expect = 4.7e-231
Identity = 450/641 (70.20%), Postives = 511/641 (79.72%), Query Frame = 0

Query: 1   MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60
           MIT  DFYHVMTA+VPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  SNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPL 120
           +N+PY M+ RF+AAD+LQK++VL +L +W K+SR G L+WTITLFSLSTLPNTLVMGIPL
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESD 180
           LKGMYG+FSG LMVQIVVLQCIIWYTLMLF+FE+RGA+ LISEQFPDTA SIVSIHV+SD
Sbjct: 121 LKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSD 180

Query: 181 VVSLDGQQVLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEIY 240
           ++SLDG+Q LETEAEIK+DGKLHVTVRRSNASRSDI+SRRSQGLS+ TPRPSNLTNAEIY
Sbjct: 181 IMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQGLSA-TPRPSNLTNAEIY 240

Query: 241 SLQSSRNPTPRGSSFNHTDFYSMLGTG---------GVAPAVSRGPTPRPSNYEEE---- 300
           SLQSSRNPTPRGSSFNHTDFYSM+ +G         G A   S+GPTPRPSNYEE+    
Sbjct: 241 SLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEEDGGPA 300

Query: 301 --NHGGGGKSGPRYYYH----GNPAPPHYPAPNMGMFSPT--GSRNKRSTANGQVGQYKS 360
                G      R++Y     G     HYPAPN GMFSP   G     +  N  V   K 
Sbjct: 301 KPTAAGTAAGAGRFHYQSGGSGGGGGAHYPAPNPGMFSPNTGGGGGTAAKGNAPVVGGKR 360

Query: 361 EDGNNNNNNNKDLHMFVWSSSASPVSDVFGGHDYGGPH---------DH-KELKLAVSPG 420
           +DG     N +DLHMFVWSSSASPVSDVFGG   GG H         DH K++K++V  G
Sbjct: 361 QDG-----NGRDLHMFVWSSSASPVSDVFGGG--GGNHHADYSTATNDHQKDVKISVPQG 420

Query: 421 KGESGPRHNDREDVEREEFSFGNRGMNS----SNNGHEMAAGEKGGTSGVSKTMPPASVM 480
                   ND + VEREEFSFGN+  +S    ++ G+ ++       +  +K MPP SVM
Sbjct: 421 NS------NDNQYVEREEFSFGNKDDDSKVLATDGGNNIS-----NKTTQAKVMPPTSVM 480

Query: 481 TRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWHVEMPAIVAQSISILSDAGLGMAMFS 540
           TRLILIMVWRKLIRNPN+YSSL G+TWSL+SF+W++EMPA++A+SISILSDAGLGMAMFS
Sbjct: 481 TRLILIMVWRKLIRNPNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLGMAMFS 540

Query: 541 LGLFMALQPRIIACGNSIATFAMAVRFLVGPAVMAAASIAVGLKGVLLHVAIVQAALPQG 600
           LGLFMAL PRIIACGN  A FA A+RF+VGPAVM  AS AVGL+GVLLHVAI+QAALPQG
Sbjct: 541 LGLFMALNPRIIACGNRRAAFAAAMRFVVGPAVMLVASYAVGLRGVLLHVAIIQAALPQG 600

Query: 601 IVPFVFAKEYNVHPDILSTAVIFGMLIALPITLVYYILLGV 607
           IVPFVFAKEYNVHPDILSTAVIFGMLIALPITL+YYILLG+
Sbjct: 601 IVPFVFAKEYNVHPDILSTAVIFGMLIALPITLLYYILLGL 622

BLAST of CmaCh11G003130 vs. ExPASy Swiss-Prot
Match: Q67UL3 (Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1C PE=2 SV=1)

HSP 1 Score: 800.0 bits (2065), Expect = 1.8e-230
Identity = 453/622 (72.83%), Postives = 505/622 (81.19%), Query Frame = 0

Query: 1   MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60
           MIT  DFYHVMTA+VPLYVAMILAYGSVKWW+IFTPDQCSGINRFVALFAVPLLSFHFI+
Sbjct: 1   MITGADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPL 120
           +N+PYTM+ RFIAADTLQK++VL +L +W+ +SRRG LEWTITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPYTMNLRFIAADTLQKLIVLALLTLWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESD 180
           LKGMYG+FSGSLMVQIVVLQCIIWYTLMLFMFE+RGAR LI+EQFPDTA +I SI V++D
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARILITEQFPDTAGAIASIVVDAD 180

Query: 181 VVSLDGQQ-VLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEI 240
           VVSLDG++ ++ETEAE+K+DGK+HVTVRRSNASRSD++SRRS G SSTTPRPSNLTNAEI
Sbjct: 181 VVSLDGRRDMIETEAEVKEDGKIHVTVRRSNASRSDVYSRRSMGFSSTTPRPSNLTNAEI 240

Query: 241 YSLQSSRNPTPRGSSFNHTDFYSMLG-----TGGVAPAVSRGPTPRPSNYEEENHGGGGK 300
           YSLQSSRNPTPRGSSFNHTDFYSM+G       G A  V  G TPRPSNYEE+      K
Sbjct: 241 YSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFAAGDAFGVRTGATPRPSNYEED-AAAPNK 300

Query: 301 SGPRYYYHGNPAPPHYPAPNMGMFSPTGSRNKRSTANGQVGQYKSEDGNNNNNNNKDLHM 360
           +G +Y          YPAPN  M +P      +  ANGQ    K EDG       KDLHM
Sbjct: 301 AGSKY--------GQYPAPNPAMAAPP---KPKKAANGQA---KGEDG-------KDLHM 360

Query: 361 FVWSSSASPVSDVFG-GHDYGGPHDHKELKLAVSPGKGESGPRHNDREDVEREEFSFGNR 420
           FVWSSSASPVSDVFG G +Y      KE+++AV      + PR  D   VER++FSFGNR
Sbjct: 361 FVWSSSASPVSDVFGNGAEYNDAAAVKEVRMAV------ASPRKAD--GVERDDFSFGNR 420

Query: 421 GM--NSSNNGHEMA-----AGEKG--GTSGVSKTMPPASVMTRLILIMVWRKLIRNPNTY 480
           G+    +  G E +     +GE G  G +     MPP SVMTRLILIMVWRKLIRNPNTY
Sbjct: 421 GVAERDAEAGDEKSVAAAVSGEHGKPGLTPAPTAMPPTSVMTRLILIMVWRKLIRNPNTY 480

Query: 481 SSLIGLTWSLVSFRWHVEMPAIVAQSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIA 540
           SSLIGL WSLV FRW+ EMPAI+ +SISILSDAGLGMAMFSLGLFMALQPRIIACGN +A
Sbjct: 481 SSLIGLIWSLVCFRWNFEMPAIILKSISILSDAGLGMAMFSLGLFMALQPRIIACGNKVA 540

Query: 541 TFAMAVRFLVGPAVMAAASIAVGLKGVLLHVAIVQAALPQGIVPFVFAKEYNVHPDILST 600
           TFAMAVRFL GPAVMAAASIAVGL+G LLHVAIVQAALPQGIVPFVFAKEY+VHPDILST
Sbjct: 541 TFAMAVRFLTGPAVMAAASIAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSVHPDILST 592

Query: 601 AVIFGMLIALPITLVYYILLGV 607
           AVIFGMLIALPITLVYYILLG+
Sbjct: 601 AVIFGMLIALPITLVYYILLGL 592

BLAST of CmaCh11G003130 vs. ExPASy Swiss-Prot
Match: Q5SMQ9 (Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PIN1A PE=2 SV=1)

HSP 1 Score: 792.7 bits (2046), Expect = 2.9e-228
Identity = 450/623 (72.23%), Postives = 497/623 (79.78%), Query Frame = 0

Query: 1   MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60
           MIT  DFYHVMTA+VPLYVAMILAYGSVKWW+IFTPDQCSGINRFVALFAVPLLSFHFI+
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWRIFTPDQCSGINRFVALFAVPLLSFHFIS 60

Query: 61  SNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPL 120
           +N+PYTM+ RFIAADTLQK+MVL +L  W+ +SRRG LEWTITLFSLSTLPNTLVMGIPL
Sbjct: 61  TNNPYTMNLRFIAADTLQKLMVLAMLTAWSHLSRRGSLEWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESD 180
           LKGMYG+FSGSLMVQIVVLQCIIWYTLMLFMFE+RGAR LI+EQFPDTAA+I SI V+ D
Sbjct: 121 LKGMYGEFSGSLMVQIVVLQCIIWYTLMLFMFEYRGARMLITEQFPDTAANIASIVVDPD 180

Query: 181 VVSLDGQQ-VLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEI 240
           VVSLDG++  +ETE E+K+DG++HVTVRRSNASRSDI+SRRS G SSTTPRPSNLTNAEI
Sbjct: 181 VVSLDGRRDAIETETEVKEDGRIHVTVRRSNASRSDIYSRRSMGFSSTTPRPSNLTNAEI 240

Query: 241 YSLQSSRNPTPRGSSFNHTDFYSMLGTG---GVAPA--VSRGPTPRPSNYEEENHGGGGK 300
           YSLQSSRNPTPRGSSFNHTDFYSM+G     G A A  V  G TPRPSNYE++       
Sbjct: 241 YSLQSSRNPTPRGSSFNHTDFYSMVGRSSNFGAADAFGVRTGATPRPSNYEDD------A 300

Query: 301 SGPRYYYHGNPAPP---HYPAPNMGMFS-PTGSRNKRSTANGQVGQYKSEDGNNNNNNNK 360
           S P+Y    + A P   HYPAPN  + S P G+  K++  NGQ                +
Sbjct: 301 SKPKYPLPASNAAPMAGHYPAPNPAVSSAPKGA--KKAATNGQA-------------KGE 360

Query: 361 DLHMFVWSSSASPVSDVFGGHDYGGPHDHKELKLAVSPGKGESGPRHNDRED-VEREEFS 420
           DLHMFVWSSSASPVSDVFG    GG  D+ +     SP K +      DRED VER++FS
Sbjct: 361 DLHMFVWSSSASPVSDVFG----GGAPDYNDAAAVKSPRKMDGA---KDREDYVERDDFS 420

Query: 421 FGNRGM--NSSNNGHEMAAGEKGG----TSGVSKTMPPASVMTRLILIMVWRKLIRNPNT 480
           FGNRG+    +  G E AA   G            MPP SVMTRLILIMVWRKLIRNPNT
Sbjct: 421 FGNRGVMDRDAEAGDEKAAAAAGADPSKAMAAPTAMPPTSVMTRLILIMVWRKLIRNPNT 480

Query: 481 YSSLIGLTWSLVSFRWHVEMPAIVAQSISILSDAGLGMAMFSLGLFMALQPRIIACGNSI 540
           YSSLIGL WSLV FRW+ EMPAIV +SISILSDAGLGMAMFSLGLFMALQP IIACGN +
Sbjct: 481 YSSLIGLIWSLVCFRWNFEMPAIVLKSISILSDAGLGMAMFSLGLFMALQPHIIACGNKV 540

Query: 541 ATFAMAVRFLVGPAVMAAASIAVGLKGVLLHVAIVQAALPQGIVPFVFAKEYNVHPDILS 600
           AT+AMAVRFL GPAVMAAAS AVGL+G LLHVAIVQAALPQGIVPFVFAKEY+VHP ILS
Sbjct: 541 ATYAMAVRFLAGPAVMAAASFAVGLRGTLLHVAIVQAALPQGIVPFVFAKEYSVHPSILS 595

Query: 601 TAVIFGMLIALPITLVYYILLGV 607
           TAVIFGMLIALPITLVYYILLG+
Sbjct: 601 TAVIFGMLIALPITLVYYILLGL 595

BLAST of CmaCh11G003130 vs. ExPASy Swiss-Prot
Match: Q8RWZ6 (Auxin efflux carrier component 4 OS=Arabidopsis thaliana OX=3702 GN=PIN4 PE=1 SV=1)

HSP 1 Score: 696.8 bits (1797), Expect = 2.1e-199
Identity = 405/642 (63.08%), Postives = 473/642 (73.68%), Query Frame = 0

Query: 1   MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60
           MIT  D Y V+TAVVPLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFI+
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPL 120
           +NDPY M+FRF+AADTLQKI++LV+LA+W  +++ G LEW IT+FSLSTLPNTLVMGIPL
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESD 180
           L  MYG ++GSLMVQ+VVLQCIIWYTL+LF+FE+RGA+ LI EQFP+T ASIVS  VESD
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 VVSLDGQQVLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEIY 240
           VVSLDG   LET+AEI +DGKLHVTVR+SNASR  +           TPRPSNLT AEIY
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLM---------MTPRPSNLTGAEIY 240

Query: 241 SLQSSRNPTPRGSSFNHTDFYSMLGTGG-----VAPA------VSRGPTPRPSNYEEENH 300
           SL S    TPRGS+FNH+DFYS++G  G       PA       SRGPTPRPSN+EE N 
Sbjct: 241 SLSS----TPRGSNFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENN- 300

Query: 301 GGGGKSGPRYYYHGN-----PAPPHYPAPNMGMFSPTGSRNKRSTANGQVGQYKSEDGNN 360
                   +Y ++ N     PA   YPAPN    + TG   K +    +  Q   E  + 
Sbjct: 301 ------AVKYGFYNNTNSSVPAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSK 360

Query: 361 NNNNNKDLHMFVWSSSASPVSDVFGGHDYGGPHDH----------KELKLAVSP------ 420
            +++ K+LHMFVWSSSASPVSDVFG    GG  D+          KE+++ VS       
Sbjct: 361 ASHDAKELHMFVWSSSASPVSDVFG----GGAGDNVATEQSEQGAKEIRMVVSDQPRKSN 420

Query: 421 ---GKGESGPRHNDREDVEREEFSFGNRGMNSSNNGH-EMAAGEKGGTSGVSKTMPPASV 480
              G  + G   +   + E E+ + G   M S++    E A G+ GG +G    MPP SV
Sbjct: 421 ARGGGDDIGGLDSGEGEREIEKATAGLNKMGSNSTAELEAAGGDGGGNNGTH--MPPTSV 480

Query: 481 MTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWHVEMPAIVAQSISILSDAGLGMAMF 540
           MTRLILIMVWRKLIRNPNTYSSLIGL W+LV++RWHV MP I+ QSISILSDAGLGMAMF
Sbjct: 481 MTRLILIMVWRKLIRNPNTYSSLIGLIWALVAYRWHVAMPKILQQSISILSDAGLGMAMF 540

Query: 541 SLGLFMALQPRIIACGNSIATFAMAVRFLVGPAVMAAASIAVGLKGVLLHVAIVQAALPQ 600
           SLGLFMALQP+IIACGNS+ATFAMAVRF+ GPA+MA A IA+GL G LL +AIVQAALPQ
Sbjct: 541 SLGLFMALQPKIIACGNSVATFAMAVRFITGPAIMAVAGIAIGLHGDLLRIAIVQAALPQ 600

Query: 601 GIVPFVFAKEYNVHPDILSTAVIFGMLIALPITLVYYILLGV 607
           GIVPFVFAKEYNVHP ILST VIFGMLIALPITLVYYILLG+
Sbjct: 601 GIVPFVFAKEYNVHPTILSTGVIFGMLIALPITLVYYILLGL 616

BLAST of CmaCh11G003130 vs. ExPASy Swiss-Prot
Match: Q9S7Z8 (Auxin efflux carrier component 3 OS=Arabidopsis thaliana OX=3702 GN=PIN3 PE=1 SV=1)

HSP 1 Score: 689.1 bits (1777), Expect = 4.5e-197
Identity = 412/658 (62.61%), Postives = 472/658 (71.73%), Query Frame = 0

Query: 1   MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60
           MI+  D Y V+TAV+PLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFI+
Sbjct: 1   MISWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPL 120
           +N+PY M+ RFIAADTLQKI++L +L +W   +R G LEW+IT+FSLSTLPNTLVMGIPL
Sbjct: 61  TNNPYAMNLRFIAADTLQKIIMLSLLVLWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESD 180
           L  MYG++SGSLMVQIVVLQCIIWYTL+LF+FEFRGA+ LI EQFP+TAASIVS  VESD
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEFRGAKMLIMEQFPETAASIVSFKVESD 180

Query: 181 VVSLDGQQVLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEIY 240
           VVSLDG   LET+AEI DDGKLHVTVR+SNA      SRRS    + TPRPSNLT AEIY
Sbjct: 181 VVSLDGHDFLETDAEIGDDGKLHVTVRKSNA------SRRSFCGPNMTPRPSNLTGAEIY 240

Query: 241 SLQSSRNPTPRGSSFNHTDFYSMLGTGG-----VAPA------VSRGPTPRPSNYEEENH 300
           SL +    TPRGS+FNH+DFY+M+G  G       PA       SRGPTPRPSN+EE   
Sbjct: 241 SLST----TPRGSNFNHSDFYNMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEE--- 300

Query: 301 GGGGKSGPRYYYHGNPAPPHYPAPNMGMFSPTGSRNKRSTANGQVGQYKSE--------- 360
                S PR+ Y+       YPAPN     P  S    STAN  V +   +         
Sbjct: 301 NCAMASSPRFGYYPGGGAGSYPAPN-----PEFSSTTTSTANKSVNKNPKDVNTNQQTTL 360

Query: 361 --DGNNNNNNNKDLHMFVWSSSASPVSD-----VFGG---HDYGGPHDH--KELKLAV-- 420
              G +N+++ K+LHMFVWSS+ SPVSD     VFGG   +D GG  D   KE+++ V  
Sbjct: 361 PTGGKSNSHDAKELHMFVWSSNGSPVSDRAGLNVFGGAPDNDQGGRSDQGAKEIRMLVPD 420

Query: 421 -----------SPGKGESGPRHN---DREDVEREEFSFGNRGMNSSNNGHEMAAGEKGGT 480
                       P  G+ G         ++ E E       G+N        A   K G 
Sbjct: 421 QSHNGETKAVAHPASGDFGGEQQFSFAGKEEEAERPKDAENGLNKLAPNSTAALQSKTGL 480

Query: 481 SGVS----KTMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWHVEMPAIVA 540
            G      K MPPASVMTRLILIMVWRKLIRNPNTYSSLIGL W+LV+FRWHV MP I+ 
Sbjct: 481 GGAEASQRKNMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLIWALVAFRWHVAMPKIIQ 540

Query: 541 QSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIATFAMAVRFLVGPAVMAAASIAVGL 600
           QSISILSDAGLGMAMFSLGLFMALQP++IACGNS+ATFAMAVRFL GPAVMA A+IA+GL
Sbjct: 541 QSISILSDAGLGMAMFSLGLFMALQPKLIACGNSVATFAMAVRFLTGPAVMAVAAIAIGL 600

Query: 601 KGVLLHVAIVQAALPQGIVPFVFAKEYNVHPDILSTAVIFGMLIALPITLVYYILLGV 607
           +G LL VAIVQAALPQGIVPFVFAKEYNVHP ILST VIFGMLIALPITLVYYILLG+
Sbjct: 601 RGDLLRVAIVQAALPQGIVPFVFAKEYNVHPAILSTGVIFGMLIALPITLVYYILLGL 640

BLAST of CmaCh11G003130 vs. TAIR 10
Match: AT1G73590.1 (Auxin efflux carrier family protein )

HSP 1 Score: 802.0 bits (2070), Expect = 3.4e-232
Identity = 450/641 (70.20%), Postives = 511/641 (79.72%), Query Frame = 0

Query: 1   MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60
           MIT  DFYHVMTA+VPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA
Sbjct: 1   MITAADFYHVMTAMVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60

Query: 61  SNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPL 120
           +N+PY M+ RF+AAD+LQK++VL +L +W K+SR G L+WTITLFSLSTLPNTLVMGIPL
Sbjct: 61  ANNPYAMNLRFLAADSLQKVIVLSLLFLWCKLSRNGSLDWTITLFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESD 180
           LKGMYG+FSG LMVQIVVLQCIIWYTLMLF+FE+RGA+ LISEQFPDTA SIVSIHV+SD
Sbjct: 121 LKGMYGNFSGDLMVQIVVLQCIIWYTLMLFLFEYRGAKLLISEQFPDTAGSIVSIHVDSD 180

Query: 181 VVSLDGQQVLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEIY 240
           ++SLDG+Q LETEAEIK+DGKLHVTVRRSNASRSDI+SRRSQGLS+ TPRPSNLTNAEIY
Sbjct: 181 IMSLDGRQPLETEAEIKEDGKLHVTVRRSNASRSDIYSRRSQGLSA-TPRPSNLTNAEIY 240

Query: 241 SLQSSRNPTPRGSSFNHTDFYSMLGTG---------GVAPAVSRGPTPRPSNYEEE---- 300
           SLQSSRNPTPRGSSFNHTDFYSM+ +G         G A   S+GPTPRPSNYEE+    
Sbjct: 241 SLQSSRNPTPRGSSFNHTDFYSMMASGGGRNSNFGPGEAVFGSKGPTPRPSNYEEDGGPA 300

Query: 301 --NHGGGGKSGPRYYYH----GNPAPPHYPAPNMGMFSPT--GSRNKRSTANGQVGQYKS 360
                G      R++Y     G     HYPAPN GMFSP   G     +  N  V   K 
Sbjct: 301 KPTAAGTAAGAGRFHYQSGGSGGGGGAHYPAPNPGMFSPNTGGGGGTAAKGNAPVVGGKR 360

Query: 361 EDGNNNNNNNKDLHMFVWSSSASPVSDVFGGHDYGGPH---------DH-KELKLAVSPG 420
           +DG     N +DLHMFVWSSSASPVSDVFGG   GG H         DH K++K++V  G
Sbjct: 361 QDG-----NGRDLHMFVWSSSASPVSDVFGGG--GGNHHADYSTATNDHQKDVKISVPQG 420

Query: 421 KGESGPRHNDREDVEREEFSFGNRGMNS----SNNGHEMAAGEKGGTSGVSKTMPPASVM 480
                   ND + VEREEFSFGN+  +S    ++ G+ ++       +  +K MPP SVM
Sbjct: 421 NS------NDNQYVEREEFSFGNKDDDSKVLATDGGNNIS-----NKTTQAKVMPPTSVM 480

Query: 481 TRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWHVEMPAIVAQSISILSDAGLGMAMFS 540
           TRLILIMVWRKLIRNPN+YSSL G+TWSL+SF+W++EMPA++A+SISILSDAGLGMAMFS
Sbjct: 481 TRLILIMVWRKLIRNPNSYSSLFGITWSLISFKWNIEMPALIAKSISILSDAGLGMAMFS 540

Query: 541 LGLFMALQPRIIACGNSIATFAMAVRFLVGPAVMAAASIAVGLKGVLLHVAIVQAALPQG 600
           LGLFMAL PRIIACGN  A FA A+RF+VGPAVM  AS AVGL+GVLLHVAI+QAALPQG
Sbjct: 541 LGLFMALNPRIIACGNRRAAFAAAMRFVVGPAVMLVASYAVGLRGVLLHVAIIQAALPQG 600

Query: 601 IVPFVFAKEYNVHPDILSTAVIFGMLIALPITLVYYILLGV 607
           IVPFVFAKEYNVHPDILSTAVIFGMLIALPITL+YYILLG+
Sbjct: 601 IVPFVFAKEYNVHPDILSTAVIFGMLIALPITLLYYILLGL 622

BLAST of CmaCh11G003130 vs. TAIR 10
Match: AT2G01420.1 (Auxin efflux carrier family protein )

HSP 1 Score: 698.4 bits (1801), Expect = 5.2e-201
Identity = 405/638 (63.48%), Postives = 473/638 (74.14%), Query Frame = 0

Query: 1   MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60
           MIT  D Y V+TAVVPLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFI+
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPL 120
           +NDPY M+FRF+AADTLQKI++LV+LA+W  +++ G LEW IT+FSLSTLPNTLVMGIPL
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESD 180
           L  MYG ++GSLMVQ+VVLQCIIWYTL+LF+FE+RGA+ LI EQFP+T ASIVS  VESD
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 VVSLDGQQVLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEIY 240
           VVSLDG   LET+AEI +DGKLHVTVR+SNASR  +           TPRPSNLT AEIY
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLM---------MTPRPSNLTGAEIY 240

Query: 241 SLQSSRNPTPRGSSFNHTDFYSMLGTGG-----VAPA------VSRGPTPRPSNYEEENH 300
           SL S    TPRGS+FNH+DFYS++G  G       PA       SRGPTPRPSN+EE N 
Sbjct: 241 SLSS----TPRGSNFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENN- 300

Query: 301 GGGGKSGPRYYYHGN-----PAPPHYPAPNMGMFSPTGSRNKRSTANGQVGQYKSEDGNN 360
                   +Y ++ N     PA   YPAPN    + TG   K +    +  Q   E  + 
Sbjct: 301 ------AVKYGFYNNTNSSVPAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSK 360

Query: 361 NNNNNKDLHMFVWSSSASPVSDVFGGHDYGGPHDH----------KELKLAVS-----PG 420
            +++ K+LHMFVWSSSASPVSDVFG    GG  D+          KE+++ VS      G
Sbjct: 361 ASHDAKELHMFVWSSSASPVSDVFG----GGAGDNVATEQSEQGAKEIRMVVSDQPRKSG 420

Query: 421 KGESGPRHNDREDVEREEFSFGNRGMNSSNNGH-EMAAGEKGGTSGVSKTMPPASVMTRL 480
             + G   +   + E E+ + G   M S++    E A G+ GG +G    MPP SVMTRL
Sbjct: 421 GDDIGGLDSGEGEREIEKATAGLNKMGSNSTAELEAAGGDGGGNNGTH--MPPTSVMTRL 480

Query: 481 ILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWHVEMPAIVAQSISILSDAGLGMAMFSLGL 540
           ILIMVWRKLIRNPNTYSSLIGL W+LV++RWHV MP I+ QSISILSDAGLGMAMFSLGL
Sbjct: 481 ILIMVWRKLIRNPNTYSSLIGLIWALVAYRWHVAMPKILQQSISILSDAGLGMAMFSLGL 540

Query: 541 FMALQPRIIACGNSIATFAMAVRFLVGPAVMAAASIAVGLKGVLLHVAIVQAALPQGIVP 600
           FMALQP+IIACGNS+ATFAMAVRF+ GPA+MA A IA+GL G LL +AIVQAALPQGIVP
Sbjct: 541 FMALQPKIIACGNSVATFAMAVRFITGPAIMAVAGIAIGLHGDLLRIAIVQAALPQGIVP 600

Query: 601 FVFAKEYNVHPDILSTAVIFGMLIALPITLVYYILLGV 607
           FVFAKEYNVHP ILST VIFGMLIALPITLVYYILLG+
Sbjct: 601 FVFAKEYNVHPTILSTGVIFGMLIALPITLVYYILLGL 612

BLAST of CmaCh11G003130 vs. TAIR 10
Match: AT2G01420.2 (Auxin efflux carrier family protein )

HSP 1 Score: 696.8 bits (1797), Expect = 1.5e-200
Identity = 405/642 (63.08%), Postives = 473/642 (73.68%), Query Frame = 0

Query: 1   MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60
           MIT  D Y V+TAVVPLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFI+
Sbjct: 1   MITWHDLYTVLTAVVPLYVAMILAYGSVQWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPL 120
           +NDPY M+FRF+AADTLQKI++LV+LA+W  +++ G LEW IT+FSLSTLPNTLVMGIPL
Sbjct: 61  TNDPYAMNFRFVAADTLQKIIMLVLLALWANLTKNGSLEWMITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESD 180
           L  MYG ++GSLMVQ+VVLQCIIWYTL+LF+FE+RGA+ LI EQFP+T ASIVS  VESD
Sbjct: 121 LIAMYGTYAGSLMVQVVVLQCIIWYTLLLFLFEYRGAKLLIMEQFPETGASIVSFKVESD 180

Query: 181 VVSLDGQQVLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEIY 240
           VVSLDG   LET+AEI +DGKLHVTVR+SNASR  +           TPRPSNLT AEIY
Sbjct: 181 VVSLDGHDFLETDAEIGNDGKLHVTVRKSNASRRSLM---------MTPRPSNLTGAEIY 240

Query: 241 SLQSSRNPTPRGSSFNHTDFYSMLGTGG-----VAPA------VSRGPTPRPSNYEEENH 300
           SL S    TPRGS+FNH+DFYS++G  G       PA       SRGPTPRPSN+EE N 
Sbjct: 241 SLSS----TPRGSNFNHSDFYSVMGFPGGRLSNFGPADLYSVQSSRGPTPRPSNFEENN- 300

Query: 301 GGGGKSGPRYYYHGN-----PAPPHYPAPNMGMFSPTGSRNKRSTANGQVGQYKSEDGNN 360
                   +Y ++ N     PA   YPAPN    + TG   K +    +  Q   E  + 
Sbjct: 301 ------AVKYGFYNNTNSSVPAAGSYPAPNPEFSTGTGVSTKPNKIPKENQQQLQEKDSK 360

Query: 361 NNNNNKDLHMFVWSSSASPVSDVFGGHDYGGPHDH----------KELKLAVSP------ 420
            +++ K+LHMFVWSSSASPVSDVFG    GG  D+          KE+++ VS       
Sbjct: 361 ASHDAKELHMFVWSSSASPVSDVFG----GGAGDNVATEQSEQGAKEIRMVVSDQPRKSN 420

Query: 421 ---GKGESGPRHNDREDVEREEFSFGNRGMNSSNNGH-EMAAGEKGGTSGVSKTMPPASV 480
              G  + G   +   + E E+ + G   M S++    E A G+ GG +G    MPP SV
Sbjct: 421 ARGGGDDIGGLDSGEGEREIEKATAGLNKMGSNSTAELEAAGGDGGGNNGTH--MPPTSV 480

Query: 481 MTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWHVEMPAIVAQSISILSDAGLGMAMF 540
           MTRLILIMVWRKLIRNPNTYSSLIGL W+LV++RWHV MP I+ QSISILSDAGLGMAMF
Sbjct: 481 MTRLILIMVWRKLIRNPNTYSSLIGLIWALVAYRWHVAMPKILQQSISILSDAGLGMAMF 540

Query: 541 SLGLFMALQPRIIACGNSIATFAMAVRFLVGPAVMAAASIAVGLKGVLLHVAIVQAALPQ 600
           SLGLFMALQP+IIACGNS+ATFAMAVRF+ GPA+MA A IA+GL G LL +AIVQAALPQ
Sbjct: 541 SLGLFMALQPKIIACGNSVATFAMAVRFITGPAIMAVAGIAIGLHGDLLRIAIVQAALPQ 600

Query: 601 GIVPFVFAKEYNVHPDILSTAVIFGMLIALPITLVYYILLGV 607
           GIVPFVFAKEYNVHP ILST VIFGMLIALPITLVYYILLG+
Sbjct: 601 GIVPFVFAKEYNVHPTILSTGVIFGMLIALPITLVYYILLGL 616

BLAST of CmaCh11G003130 vs. TAIR 10
Match: AT1G70940.1 (Auxin efflux carrier family protein )

HSP 1 Score: 689.1 bits (1777), Expect = 3.2e-198
Identity = 412/658 (62.61%), Postives = 472/658 (71.73%), Query Frame = 0

Query: 1   MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60
           MI+  D Y V+TAV+PLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFI+
Sbjct: 1   MISWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPL 120
           +N+PY M+ RFIAADTLQKI++L +L +W   +R G LEW+IT+FSLSTLPNTLVMGIPL
Sbjct: 61  TNNPYAMNLRFIAADTLQKIIMLSLLVLWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESD 180
           L  MYG++SGSLMVQIVVLQCIIWYTL+LF+FEFRGA+ LI EQFP+TAASIVS  VESD
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEFRGAKMLIMEQFPETAASIVSFKVESD 180

Query: 181 VVSLDGQQVLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEIY 240
           VVSLDG   LET+AEI DDGKLHVTVR+SNA      SRRS    + TPRPSNLT AEIY
Sbjct: 181 VVSLDGHDFLETDAEIGDDGKLHVTVRKSNA------SRRSFCGPNMTPRPSNLTGAEIY 240

Query: 241 SLQSSRNPTPRGSSFNHTDFYSMLGTGG-----VAPA------VSRGPTPRPSNYEEENH 300
           SL +    TPRGS+FNH+DFY+M+G  G       PA       SRGPTPRPSN+EE   
Sbjct: 241 SLST----TPRGSNFNHSDFYNMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEE--- 300

Query: 301 GGGGKSGPRYYYHGNPAPPHYPAPNMGMFSPTGSRNKRSTANGQVGQYKSE--------- 360
                S PR+ Y+       YPAPN     P  S    STAN  V +   +         
Sbjct: 301 NCAMASSPRFGYYPGGGAGSYPAPN-----PEFSSTTTSTANKSVNKNPKDVNTNQQTTL 360

Query: 361 --DGNNNNNNNKDLHMFVWSSSASPVSD-----VFGG---HDYGGPHDH--KELKLAV-- 420
              G +N+++ K+LHMFVWSS+ SPVSD     VFGG   +D GG  D   KE+++ V  
Sbjct: 361 PTGGKSNSHDAKELHMFVWSSNGSPVSDRAGLNVFGGAPDNDQGGRSDQGAKEIRMLVPD 420

Query: 421 -----------SPGKGESGPRHN---DREDVEREEFSFGNRGMNSSNNGHEMAAGEKGGT 480
                       P  G+ G         ++ E E       G+N        A   K G 
Sbjct: 421 QSHNGETKAVAHPASGDFGGEQQFSFAGKEEEAERPKDAENGLNKLAPNSTAALQSKTGL 480

Query: 481 SGVS----KTMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLTWSLVSFRWHVEMPAIVA 540
            G      K MPPASVMTRLILIMVWRKLIRNPNTYSSLIGL W+LV+FRWHV MP I+ 
Sbjct: 481 GGAEASQRKNMPPASVMTRLILIMVWRKLIRNPNTYSSLIGLIWALVAFRWHVAMPKIIQ 540

Query: 541 QSISILSDAGLGMAMFSLGLFMALQPRIIACGNSIATFAMAVRFLVGPAVMAAASIAVGL 600
           QSISILSDAGLGMAMFSLGLFMALQP++IACGNS+ATFAMAVRFL GPAVMA A+IA+GL
Sbjct: 541 QSISILSDAGLGMAMFSLGLFMALQPKLIACGNSVATFAMAVRFLTGPAVMAVAAIAIGL 600

Query: 601 KGVLLHVAIVQAALPQGIVPFVFAKEYNVHPDILSTAVIFGMLIALPITLVYYILLGV 607
           +G LL VAIVQAALPQGIVPFVFAKEYNVHP ILST VIFGMLIALPITLVYYILLG+
Sbjct: 601 RGDLLRVAIVQAALPQGIVPFVFAKEYNVHPAILSTGVIFGMLIALPITLVYYILLGL 640

BLAST of CmaCh11G003130 vs. TAIR 10
Match: AT1G23080.3 (Auxin efflux carrier family protein )

HSP 1 Score: 686.8 bits (1771), Expect = 1.6e-197
Identity = 406/635 (63.94%), Postives = 469/635 (73.86%), Query Frame = 0

Query: 1   MITLLDFYHVMTAVVPLYVAMILAYGSVKWWKIFTPDQCSGINRFVALFAVPLLSFHFIA 60
           MIT  D Y V+TAV+PLYVAMILAYGSV+WWKIF+PDQCSGINRFVA+FAVPLLSFHFI+
Sbjct: 1   MITWHDLYTVLTAVIPLYVAMILAYGSVRWWKIFSPDQCSGINRFVAIFAVPLLSFHFIS 60

Query: 61  SNDPYTMSFRFIAADTLQKIMVLVVLAIWTKVSRRGCLEWTITLFSLSTLPNTLVMGIPL 120
           SN+PY M+ RFIAADTLQK+++L +L IW   +R G LEW+IT+FSLSTLPNTLVMGIPL
Sbjct: 61  SNNPYAMNLRFIAADTLQKLIMLTLLIIWANFTRSGSLEWSITIFSLSTLPNTLVMGIPL 120

Query: 121 LKGMYGDFSGSLMVQIVVLQCIIWYTLMLFMFEFRGARNLISEQFPDTAASIVSIHVESD 180
           L  MYG++SGSLMVQIVVLQCIIWYTL+LF+FE+RGA+ LI EQFP+T ASIVS  VESD
Sbjct: 121 LIAMYGEYSGSLMVQIVVLQCIIWYTLLLFLFEYRGAKILIMEQFPETGASIVSFKVESD 180

Query: 181 VVSLDGQQVLETEAEIKDDGKLHVTVRRSNASRSDIFSRRSQGLSSTTPRPSNLTNAEIY 240
           VVSLDG   LET+A+I DDGKLHVTVR+SNASR   +     G ++ TPRPSNLT AEIY
Sbjct: 181 VVSLDGHDFLETDAQIGDDGKLHVTVRKSNASRRSFY---GGGGTNMTPRPSNLTGAEIY 240

Query: 241 SLQSSRNPTPRGSSFNHTDFYSMLGTGG-----VAPA------VSRGPTPRPSNYEEENH 300
           SL    N TPRGS+FNH+DFYSM+G  G       PA       SRGPTPRPSN+EE   
Sbjct: 241 SL----NTTPRGSNFNHSDFYSMMGFPGGRLSNFGPADMYSVQSSRGPTPRPSNFEE--- 300

Query: 301 GGGGKSGPRYYYHGNPAPPHYPAPNMGMFSPTGSRNKRSTANGQVGQYKSEDGNNNNNNN 360
                S PR+ Y+   AP  YPAPN    +   + +K    N          G +N+N+ 
Sbjct: 301 SCAMASSPRFGYYPGGAPGSYPAPNPEFSTGNKTGSKAPKEN------HHHVGKSNSNDA 360

Query: 361 KDLHMFVWSSSASPVSD------------VFGGHDYGGPHDHKELKLAVSPGKGESGPRH 420
           K+LHMFVW S+ SPVSD              G  D GG    KE+++ +S     +GP +
Sbjct: 361 KELHMFVWGSNGSPVSDRAGLQVDNGANEQVGKSDQGGA---KEIRMLISDHTQNAGPMN 420

Query: 421 ND---REDVER-EEFSFGNRGM--NSSNNGHEMAAGEKGGTSGVSKTMPPASVMTRLILI 480
            D    E+ ER +E   G   +  NS+   +   A E G T  V K MPPASVMTRLILI
Sbjct: 421 GDYGGEEESERVKEVPNGLHKLRCNSTAELNPKEAIETGETVPV-KHMPPASVMTRLILI 480

Query: 481 MVWRKLIRNPNTYSSLIGLTWSLVSFRWHVEMPAIVAQSISILSDAGLGMAMFSLGLFMA 540
           MVWRKLIRNPNTYSSLIGL W+LV+FRW V MP I+ QSISILSDAGLGMAMFSLGLFMA
Sbjct: 481 MVWRKLIRNPNTYSSLIGLIWALVAFRWDVAMPKIIQQSISILSDAGLGMAMFSLGLFMA 540

Query: 541 LQPRIIACGNSIATFAMAVRFLVGPAVMAAASIAVGLKGVLLHVAIVQAALPQGIVPFVF 600
           LQP++IACGNS ATFAMAVRF  GPAVMA A++A+GL+G LL VAIVQAALPQGIVPFVF
Sbjct: 541 LQPKLIACGNSTATFAMAVRFFTGPAVMAVAAMAIGLRGDLLRVAIVQAALPQGIVPFVF 600

Query: 601 AKEYNVHPDILSTAVIFGMLIALPITLVYYILLGV 607
           AKEYNVHP ILST VIFGMLIALPITLVYYILLG+
Sbjct: 601 AKEYNVHPAILSTGVIFGMLIALPITLVYYILLGL 615

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9C6B84.7e-23170.20Auxin efflux carrier component 1 OS=Arabidopsis thaliana OX=3702 GN=PIN1 PE=1 SV... [more]
Q67UL31.8e-23072.83Probable auxin efflux carrier component 1c OS=Oryza sativa subsp. japonica OX=39... [more]
Q5SMQ92.9e-22872.23Auxin efflux carrier component 1a OS=Oryza sativa subsp. japonica OX=39947 GN=PI... [more]
Q8RWZ62.1e-19963.08Auxin efflux carrier component 4 OS=Arabidopsis thaliana OX=3702 GN=PIN4 PE=1 SV... [more]
Q9S7Z84.5e-19762.61Auxin efflux carrier component 3 OS=Arabidopsis thaliana OX=3702 GN=PIN3 PE=1 SV... [more]
Match NameE-valueIdentityDescription
AT1G73590.13.4e-23270.20Auxin efflux carrier family protein [more]
AT2G01420.15.2e-20163.48Auxin efflux carrier family protein [more]
AT2G01420.21.5e-20063.08Auxin efflux carrier family protein [more]
AT1G70940.13.2e-19862.61Auxin efflux carrier family protein [more]
AT1G23080.31.6e-19763.94Auxin efflux carrier family protein [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
IPR004776Membrane transport proteinPFAMPF03547Mem_transcoord: 9..601
e-value: 7.0E-197
score: 653.4
IPR014024Auxin efflux carrier, plant typeTIGRFAMTIGR00946TIGR00946coord: 1..605
e-value: 2.6E-163
score: 540.9
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 318..348
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 266..348
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 410..437
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 213..235
NoneNo IPR availablePANTHERPTHR31752AUXIN EFFLUX CARRIER COMPONENT 1B-RELATEDcoord: 1..606
NoneNo IPR availablePANTHERPTHR31752:SF48AUXIN EFFLUX CARRIER COMPONENT 1Acoord: 1..606

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G003130.1CmaCh11G003130.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0010315 auxin efflux
biological_process GO:0010252 auxin homeostasis
biological_process GO:0009926 auxin polar transport
biological_process GO:0055085 transmembrane transport
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane
cellular_component GO:0005886 plasma membrane
molecular_function GO:0010329 auxin efflux transmembrane transporter activity