CmaCh11G001980 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh11G001980
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionFlavonoid 3' hydroxylase
LocationCma_Chr11: 1007866 .. 1010701 (+)
RNA-Seq ExpressionCmaCh11G001980
SyntenyCmaCh11G001980
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
GCTTTTTATTTTGTCGGCTTTTACGTTCGTTAAAACTTTCTTGTCGGTAGACTGTAAATATGTTTCTTTTAACGGGTTTAATATATCGTGGACTCTTATCTGGCTCCCTTCGTACCTACTTCATAGCCCAAATTACAAATTTACCCCCGAACCATTCAATTCAAAGCCCCATTTAAGCGTCTCCTTCCTCGTCCCTTTGATCCGTGAAATCCGCCAACTGCCCAGACCAATACCATTAAAAATATCATGGACGAAATTTCCCTCTTCTTCTCTGCCGCCATAACCTTGGCCACCGTCGTCCTCCTCTACCGCCTTTTATTCCACCAACGAAATTCCCGATCTCTCCCTCTCCCGCCGGGCCCGAAACCATGGCCGACAGTCGGAAACTTGCCGCAAATTGGCGGAGCGCCTCTCCACTCCCAACTCGCCGCCATGTCCGGAGAGTACGGACCTCTGATGTACCTCCGATTCGGCTCTATCGATGTCGTTGTCGCGGCATCGGCAGCAATAGCAGAGCAATTCTTGAAGATTCGAGACACTAATTTCCTCAGCCGGCCGCCGAACGCCGGCGCTAAATATATAGCATATAATTATCAGGACATGGTTTTTGCACCGTATGGTCCACGGTGGCGGTTACTCCGGAAGATCAGTGCCGTCCATCTGTTCTCCTCAAAAGCCCTAGAGGATAATCGGTGCGTACGCGAGGTTCGTCGGTTTCTTCTTCTCATCATTTATGTGCATGCATGCCTGAGTTTTGACCGAATTATTTTCCCGATCAATCATTAAATTGCGTTTCGTTTTGGGAGGAAAAAAAGTAATGACGATGATGCAAGCAAGGTTGGTTTCCGTACAAATTTTCTTAAAGAGGTTTAAAATTTTTCATTACTGTATAGATGATCTTTATTCACAAGATTTTTAATAAAAGGAAACAAACTTGAACCTGTCACGTTTGTCGTTAATGCCGTACGCTTTAAAACGCCAACTAAATCTTTCCAATTTTGCATTATTAGCCATAAAAACTGCTGACTCACTTTGAGAATGACGTGGACTAGTATTTTGTCTTTGAAAATATTAAAATTTTGTTTCAATAATATCCTTTTAGATGAAAAATGTCAAAATTTCTTTTTATTAATATCATCTTTACCAATTTTTCCTATAAAACATTTGAAAGGTGGATGAAATAAGCTCTAAAATTCATAAATCATAATTAGGAACAAATAAAAAGGCTAAATTGTTTTTGGAATGGAGTTTGGAAGCGTTGACAGGTGAAATGAGAGGTAGGGGACCGATTTCTTGTTTGAATTGATTTAAAGTTCAACTACCCATACTGGAAGATCATTTATTACTCTGTTTTAAAGTGGAGGGTTTTTATTTACCAGTTATTATTGTAGGGTACTGCGAGTATTGCCCACAAAGTTTACTCAATTATTCTTATTTTACTTTCCAAGCTAGGTTGGGTTGAAGCTATATAAATAAATGTTACTAATTATCCTCCTACCTTTAACTTCCAAAGTACAGGAGGAGGTGGGGGTGCTGGTACAGGCCCTTGCAAGGCCCGGAGAGGCACCGGCCACCTTGGGGAGTTTGCTCACTGTGTGCACAGCCAATGCCCTCGGGCGGACGATGATCGGCCGACGTGTTTTCGGCGATGGCAGTGGCGGTGGCGATCCAATGTCAGACCAGTTTAAGGCGATGGTAGAACAAGTTATGATTCTGTCCGGAAAGTTCAACCCCGGCAACTTTTTCCCGTCGCTTGAGTGGCTTGATTTGCTGGGCGTTGCTAGAGAGATGAAAAAGGTCCACAAATGGTTCGATGATTTATTGTCCAAGATCCTGGAAGAGCACCGAACTTTATTGGCCAGTGGCGGCGAACACCAAGACTTGCTGAGCACGCTTCTGTCCACAAGGGACCACGCTGAAGATGAAAATGAGAAGCTCAATGACACTGAGATCAAAGCTTTGCTATTGGTTTTCATTTCTATTTAAATTTAGTATTATTTTTTAAAGAAAATCTCTAAAATTCTTTTCCTTTTCTACCCTTCAATTTTGTTAACTAAAAGGGAAGGATCTGTGTTTCATTTTCATGCAGAATATGTTCACAGCTGGAACCGACACGTCAGCAAGCACAGTCGAATGGGCCTTGGCGGAGCTCATCAGACACCCAAAAATGATGTACCAGGCCCAACAAGAACTGGACTCTGTAGTGGGCCGTGATCGGGTAGTTTCTGATCTAGACCTGCCGAAGCTCGGCTATCTCCAAGCGGTGGTGAAGGAAACATTCCGTCTCCACCCACCAACGCCGCTCTCCCTCCCCCGCATGGCTGCCGAGAGCTGCGAGATCAACGGCTACCATATCCCTAAAGGCTCCACTCTGTTGGTGGACGTATGGGCCATAAGCCGTGACCCGAAAGAATGGACATGCCCACTCGAGTTTAGGCCCGATAGGTTCCTCCCCGGTGGCGAGAAGGCCCACGTCGATGTGAAGGGAAATGACTTTGGGGTGATTCCGTTTGGGGCTGGACGGAGGATCTGCGTGGGGATGAGTATGGGCCTGAGAATGGTCCAGATGTTAACTGCCTCGCTGGTCCATTCGTTTGATTGGGCCTTGCCTAGCGGGCTTTCGCCCGGTGAGTTGAACATGGACGAGGCCTTTGGACTGACTCTGCGTCGGGCCCAGCCCTTGGTTGCGCATCCTCGGCCCAGATTATCGCCTGGGGTTTATCAATGATGTTTTTTACTTCAACCGTTTAGAAGATTTATGATGGGTTAAATATAATGTTCAAACTTTTAAAAATATAAAATATTTTTAAAATAATGGAGAAAATCTATTGGCACA

mRNA sequence

GCTTTTTATTTTGTCGGCTTTTACGTTCGTTAAAACTTTCTTGTCGGTAGACTGTAAATATGTTTCTTTTAACGGGTTTAATATATCGTGGACTCTTATCTGGCTCCCTTCGTACCTACTTCATAGCCCAAATTACAAATTTACCCCCGAACCATTCAATTCAAAGCCCCATTTAAGCGTCTCCTTCCTCGTCCCTTTGATCCGTGAAATCCGCCAACTGCCCAGACCAATACCATTAAAAATATCATGGACGAAATTTCCCTCTTCTTCTCTGCCGCCATAACCTTGGCCACCGTCGTCCTCCTCTACCGCCTTTTATTCCACCAACGAAATTCCCGATCTCTCCCTCTCCCGCCGGGCCCGAAACCATGGCCGACAGTCGGAAACTTGCCGCAAATTGGCGGAGCGCCTCTCCACTCCCAACTCGCCGCCATGTCCGGAGAGTACGGACCTCTGATGTACCTCCGATTCGGCTCTATCGATGTCGTTGTCGCGGCATCGGCAGCAATAGCAGAGCAATTCTTGAAGATTCGAGACACTAATTTCCTCAGCCGGCCGCCGAACGCCGGCGCTAAATATATAGCATATAATTATCAGGACATGGTTTTTGCACCGTATGGTCCACGGTGGCGGTTACTCCGGAAGATCAGTGCCGTCCATCTGTTCTCCTCAAAAGCCCTAGAGGATAATCGGTGCGTACGCGAGGAGGAGGTGGGGGTGCTGGTACAGGCCCTTGCAAGGCCCGGAGAGGCACCGGCCACCTTGGGGAGTTTGCTCACTGTGTGCACAGCCAATGCCCTCGGGCGGACGATGATCGGCCGACGTGTTTTCGGCGATGGCAGTGGCGGTGGCGATCCAATGTCAGACCAGTTTAAGGCGATGGTAGAACAAGTTATGATTCTGTCCGGAAAGTTCAACCCCGGCAACTTTTTCCCGTCGCTTGAGTGGCTTGATTTGCTGGGCGTTGCTAGAGAGATGAAAAAGGTCCACAAATGGTTCGATGATTTATTGTCCAAGATCCTGGAAGAGCACCGAACTTTATTGGCCAGTGGCGGCGAACACCAAGACTTGCTGAGCACGCTTCTGTCCACAAGGGACCACGCTGAAGATGAAAATGAGAAGCTCAATGACACTGAGATCAAAGCTTTGCTATTGAATATGTTCACAGCTGGAACCGACACGTCAGCAAGCACAGTCGAATGGGCCTTGGCGGAGCTCATCAGACACCCAAAAATGATGTACCAGGCCCAACAAGAACTGGACTCTGTAGTGGGCCGTGATCGGGTAGTTTCTGATCTAGACCTGCCGAAGCTCGGCTATCTCCAAGCGGTGGTGAAGGAAACATTCCGTCTCCACCCACCAACGCCGCTCTCCCTCCCCCGCATGGCTGCCGAGAGCTGCGAGATCAACGGCTACCATATCCCTAAAGGCTCCACTCTGTTGGTGGACGTATGGGCCATAAGCCGTGACCCGAAAGAATGGACATGCCCACTCGAGTTTAGGCCCGATAGGTTCCTCCCCGGTGGCGAGAAGGCCCACGTCGATGTGAAGGGAAATGACTTTGGGGTGATTCCGTTTGGGGCTGGACGGAGGATCTGCGTGGGGATGAGTATGGGCCTGAGAATGGTCCAGATGTTAACTGCCTCGCTGGTCCATTCGTTTGATTGGGCCTTGCCTAGCGGGCTTTCGCCCGGTGAGTTGAACATGGACGAGGCCTTTGGACTGACTCTGCGTCGGGCCCAGCCCTTGGTTGCGCATCCTCGGCCCAGATTATCGCCTGGGGTTTATCAATGATGTTTTTTACTTCAACCGTTTAGAAGATTTATGATGGGTTAAATATAATGTTCAAACTTTTAAAAATATAAAATATTTTTAAAATAATGGAGAAAATCTATTGGCACA

Coding sequence (CDS)

ATGGACGAAATTTCCCTCTTCTTCTCTGCCGCCATAACCTTGGCCACCGTCGTCCTCCTCTACCGCCTTTTATTCCACCAACGAAATTCCCGATCTCTCCCTCTCCCGCCGGGCCCGAAACCATGGCCGACAGTCGGAAACTTGCCGCAAATTGGCGGAGCGCCTCTCCACTCCCAACTCGCCGCCATGTCCGGAGAGTACGGACCTCTGATGTACCTCCGATTCGGCTCTATCGATGTCGTTGTCGCGGCATCGGCAGCAATAGCAGAGCAATTCTTGAAGATTCGAGACACTAATTTCCTCAGCCGGCCGCCGAACGCCGGCGCTAAATATATAGCATATAATTATCAGGACATGGTTTTTGCACCGTATGGTCCACGGTGGCGGTTACTCCGGAAGATCAGTGCCGTCCATCTGTTCTCCTCAAAAGCCCTAGAGGATAATCGGTGCGTACGCGAGGAGGAGGTGGGGGTGCTGGTACAGGCCCTTGCAAGGCCCGGAGAGGCACCGGCCACCTTGGGGAGTTTGCTCACTGTGTGCACAGCCAATGCCCTCGGGCGGACGATGATCGGCCGACGTGTTTTCGGCGATGGCAGTGGCGGTGGCGATCCAATGTCAGACCAGTTTAAGGCGATGGTAGAACAAGTTATGATTCTGTCCGGAAAGTTCAACCCCGGCAACTTTTTCCCGTCGCTTGAGTGGCTTGATTTGCTGGGCGTTGCTAGAGAGATGAAAAAGGTCCACAAATGGTTCGATGATTTATTGTCCAAGATCCTGGAAGAGCACCGAACTTTATTGGCCAGTGGCGGCGAACACCAAGACTTGCTGAGCACGCTTCTGTCCACAAGGGACCACGCTGAAGATGAAAATGAGAAGCTCAATGACACTGAGATCAAAGCTTTGCTATTGAATATGTTCACAGCTGGAACCGACACGTCAGCAAGCACAGTCGAATGGGCCTTGGCGGAGCTCATCAGACACCCAAAAATGATGTACCAGGCCCAACAAGAACTGGACTCTGTAGTGGGCCGTGATCGGGTAGTTTCTGATCTAGACCTGCCGAAGCTCGGCTATCTCCAAGCGGTGGTGAAGGAAACATTCCGTCTCCACCCACCAACGCCGCTCTCCCTCCCCCGCATGGCTGCCGAGAGCTGCGAGATCAACGGCTACCATATCCCTAAAGGCTCCACTCTGTTGGTGGACGTATGGGCCATAAGCCGTGACCCGAAAGAATGGACATGCCCACTCGAGTTTAGGCCCGATAGGTTCCTCCCCGGTGGCGAGAAGGCCCACGTCGATGTGAAGGGAAATGACTTTGGGGTGATTCCGTTTGGGGCTGGACGGAGGATCTGCGTGGGGATGAGTATGGGCCTGAGAATGGTCCAGATGTTAACTGCCTCGCTGGTCCATTCGTTTGATTGGGCCTTGCCTAGCGGGCTTTCGCCCGGTGAGTTGAACATGGACGAGGCCTTTGGACTGACTCTGCGTCGGGCCCAGCCCTTGGTTGCGCATCCTCGGCCCAGATTATCGCCTGGGGTTTATCAATGA

Protein sequence

MDEISLFFSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQLAAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVCTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGVAREMKKVHKWFDDLLSKILEEHRTLLASGGEHQDLLSTLLSTRDHAEDENEKLNDTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVYQ
Homology
BLAST of CmaCh11G001980 vs. ExPASy Swiss-Prot
Match: Q9SBQ9 (Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1)

HSP 1 Score: 658.3 bits (1697), Expect = 7.2e-188
Identity = 323/515 (62.72%), Postives = 402/515 (78.06%), Query Frame = 0

Query: 1   MDEISLFFSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQL 60
           M+ +SL     I    +  + R  F +R    LPLPPGPKPWP +GNL  +G  P H   
Sbjct: 1   MEILSLILYTVIFSFLLQFILRSFFRKR--YPLPLPPGPKPWPIIGNLVHLGPKP-HQST 60

Query: 61  AAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 120
           AAM+  YGPLMYL+ G +DVVVAASA++A QFLK  D NF SRPPN+GA+++AYNYQD+V
Sbjct: 61  AAMAQTYGPLMYLKMGFVDVVVAASASVAAQFLKTHDANFSSRPPNSGAEHMAYNYQDLV 120

Query: 121 FAPYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVC 180
           FAPYGPRWR+LRKI +VHLFS+KAL+D R VR++EV  L +ALA  G+ P  LG LL VC
Sbjct: 121 FAPYGPRWRMLRKICSVHLFSTKALDDFRHVRQDEVKTLTRALASAGQKPVKLGQLLNVC 180

Query: 181 TANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGV 240
           T NAL R M+G+RVF DGSG  DP + +FK+MV ++M+++G FN G+F P L WLD+ GV
Sbjct: 181 TTNALARVMLGKRVFADGSGDVDPQAAEFKSMVVEMMVVAGVFNIGDFIPQLNWLDIQGV 240

Query: 241 AREMKKVHKWFDDLLSKILEEHRTLLASGGEHQDLLSTLLSTR-DHAEDENEKLNDTEIK 300
           A +MKK+H  FD  L+ ILEEH+  +   GE +DLLSTL+S + D A+++  KL DTEIK
Sbjct: 241 AAKMKKLHARFDAFLTDILEEHKGKIF--GEMKDLLSTLISLKNDDADNDGGKLTDTEIK 300

Query: 301 ALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYL 360
           ALLLN+F AGTDTS+STVEWA+AELIR+PK++ QAQQE+D VVGRDR+V +LDL +L YL
Sbjct: 301 ALLLNLFVAGTDTSSSTVEWAIAELIRNPKILAQAQQEIDKVVGRDRLVGELDLAQLTYL 360

Query: 361 QAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFR 420
           +A+VKETFRLHP TPLSLPR+A+ESCEINGY IPKGSTLL++VWAI+RDP  W  PLEFR
Sbjct: 361 EAIVKETFRLHPSTPLSLPRIASESCEINGYFIPKGSTLLLNVWAIARDPNAWADPLEFR 420

Query: 421 PDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSG 480
           P+RFLPGGEK  VDV+GNDF VIPFGAGRRIC GM++G+RMVQ++ A+L+H+F+W L SG
Sbjct: 421 PERFLPGGEKPKVDVRGNDFEVIPFGAGRRICAGMNLGIRMVQLMIATLIHAFNWDLVSG 480

Query: 481 LSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVY 515
             P  LNM+EA+GLTL+RA PLV HPRPRL    Y
Sbjct: 481 QLPEMLNMEEAYGLTLQRADPLVVHPRPRLEAQAY 510

BLAST of CmaCh11G001980 vs. ExPASy Swiss-Prot
Match: Q9SD85 (Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1)

HSP 1 Score: 647.9 bits (1670), Expect = 9.7e-185
Identity = 318/506 (62.85%), Postives = 399/506 (78.85%), Query Frame = 0

Query: 12  ITLATVV-LLYRLLFHQRN-SRSLPLPPGPKPWPTVGNLPQIGGAPLHSQLAAMSGEYGP 71
           I LATV+ L+ R+  H+RN S +  LPPGP PWP +GNLP +G  P H  L+AM   YGP
Sbjct: 8   ILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSAMVTTYGP 67

Query: 72  LMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWR 131
           +++LR G +DVVVAAS ++AEQFLKI D NF SRPPN+GAK++AYNYQD+VFAPYG RWR
Sbjct: 68  ILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWR 127

Query: 132 LLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVCTANALGRTM 191
           LLRKIS+VHLFS+KALED + VR+EEVG L + L R G  P  LG L+ +C  NALGR M
Sbjct: 128 LLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGREM 187

Query: 192 IGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGVAREMKKVHK 251
           IGRR+FG      D  +D+F++MV ++M L+G FN G+F PSL+WLDL GVA +MK++HK
Sbjct: 188 IGRRLFG---ADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHK 247

Query: 252 WFDDLLSKILEEHRTLLASGGEHQDLLSTLLSTR-DHAEDENEKLNDTEIKALLLNMFTA 311
            FD  LS IL+EH  +     +H D+LSTL+S +    + +   L DTEIKALLLNMFTA
Sbjct: 248 RFDAFLSSILKEHE-MNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFTA 307

Query: 312 GTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFR 371
           GTDTSASTV+WA+AELIRHP +M +AQ+ELD VVGRDR V++ D+ +L YLQAV+KE FR
Sbjct: 308 GTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFR 367

Query: 372 LHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPDRFLPGGE 431
           LHPPTPLSLP +A+ESCEINGYHIPKGSTLL ++WAI+RDP +W+ PL F+P+RFLPGGE
Sbjct: 368 LHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGE 427

Query: 432 KAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMD 491
           K+ VDVKG+DF +IPFGAGRRIC G+S+GLR +Q LTA+LV  FDW L  G++P +LNM+
Sbjct: 428 KSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNME 487

Query: 492 EAFGLTLRRAQPLVAHPRPRLSPGVY 515
           E++GLTL+RA PLV HP+PRL+P VY
Sbjct: 488 ESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of CmaCh11G001980 vs. ExPASy Swiss-Prot
Match: A0A4D6Q415 (Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B137 PE=1 SV=1)

HSP 1 Score: 596.3 bits (1536), Expect = 3.3e-169
Identity = 296/513 (57.70%), Postives = 379/513 (73.88%), Query Frame = 0

Query: 4   ISLFFSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQLAAM 63
           ++ FF    TL     +  LL+ +R+++  PLPPGP  WP +GNLPQ+G  P H  L A+
Sbjct: 2   LTFFFLWISTLLLSSFIVYLLYRRRSAQCPPLPPGPNGWPILGNLPQLGAKP-HQTLDAL 61

Query: 64  SGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAP 123
           S +YGPL  LR GS++VVVA+S+A+A QFL+  D NF +RPPN+GA+++AYNYQD+VFAP
Sbjct: 62  SKQYGPLFRLRLGSVNVVVASSSAVAAQFLRTHDVNFSNRPPNSGAEHVAYNYQDLVFAP 121

Query: 124 YGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAP--ATLGSLLTVCT 183
           YGPRWR+LRK+ +VHLFS KAL+D R VR+ EV  LV+ L R  +      LG  L VC 
Sbjct: 122 YGPRWRMLRKLCSVHLFSLKALDDLRPVRQGEVACLVRNLRRHADTGVLVNLGKALNVCA 181

Query: 184 ANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGVA 243
            NAL R M+GRRVF D        +D+FK MV ++M L+G FN G+F P L WLDL GV 
Sbjct: 182 TNALARAMLGRRVFAD-EDAQLAEADEFKEMVVELMRLAGVFNVGDFVPGLGWLDLQGVV 241

Query: 244 REMKKVHKWFDDLLSKILEEHRTLLASGGEHQDLLSTLLSTRDHAEDENEKLNDTEIKAL 303
            +MK++H+ +D  L +++EE++    SG    DLLS L+  ++   +   KLN+T+IKAL
Sbjct: 242 GKMKRLHRRYDAFLDRVIEENQANAKSG----DLLSVLIRLKEADAEGEIKLNNTDIKAL 301

Query: 304 LLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQA 363
           LLN+FTAGTDTS+STVEW LAELIRHP ++ + Q ELDSV+GRDR+V++ DLP L YLQA
Sbjct: 302 LLNLFTAGTDTSSSTVEWVLAELIRHPDILQKTQHELDSVIGRDRLVAESDLPNLPYLQA 361

Query: 364 VVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPD 423
           VVKETFRLHP TPLSLPRMA+E C +NGY IPK +TLLV+VW+I RD   W  PLEFRP 
Sbjct: 362 VVKETFRLHPSTPLSLPRMASEECIVNGYKIPKHATLLVNVWSIGRDAAVWNDPLEFRPS 421

Query: 424 RFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLS 483
           RFLPGGE+ HVDVKGNDF VIPFGAGRRIC G+S+GLRMVQ +TA++VH++DW+LP G  
Sbjct: 422 RFLPGGEREHVDVKGNDFEVIPFGAGRRICAGLSLGLRMVQFMTATIVHAYDWSLPKGQE 481

Query: 484 PGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVY 515
             +L+M+EA+GLTL+RA PL+  P PRLS   Y
Sbjct: 482 CQKLDMEEAYGLTLQRAVPLMVQPIPRLSHKAY 508

BLAST of CmaCh11G001980 vs. ExPASy Swiss-Prot
Match: Q7G602 (Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=CYP75B3 PE=2 SV=1)

HSP 1 Score: 574.3 bits (1479), Expect = 1.4e-162
Identity = 281/489 (57.46%), Postives = 362/489 (74.03%), Query Frame = 0

Query: 34  PLPPGPKPWPTVGNLPQIGGAPLHSQLAAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFL 93
           PLPPGP+ WP +GNLPQ+G  P H  + A++ +YGPL  LRFG  +VVVAASA +A QFL
Sbjct: 40  PLPPGPRGWPVLGNLPQLGDKP-HHTMCALARQYGPLFRLRFGCAEVVVAASAPVAAQFL 99

Query: 94  KIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRKISAVHLFSSKALEDNRCVRE 153
           +  D NF +RPPN+GA+++AYNYQD+VFAPYG RWR LRK+ A+HLFS+KAL+D R VRE
Sbjct: 100 RGHDANFSNRPPNSGAEHVAYNYQDLVFAPYGARWRALRKLCALHLFSAKALDDLRAVRE 159

Query: 154 EEVGVLVQALARPGEAPATLGSLLTVCTANALGRTMIGRRVFGDGSGGGDPMSDQFKAMV 213
            EV ++V+ LAR   A   LG    VC  N L R  IG RVF    G G   + +FK MV
Sbjct: 160 GEVALMVRNLARQQAASVALGQEANVCATNTLARATIGHRVFAVDGGEG---AREFKEMV 219

Query: 214 EQVMILSGKFNPGNFFPSLEWLDLLGVAREMKKVHKWFDDLLSKILEEHRT-----LLAS 273
            ++M L+G FN G+F P+L WLD  GV  +MK++H+ +D++++  + E +       +A+
Sbjct: 220 VELMQLAGVFNVGDFVPALRWLDPQGVVAKMKRLHRRYDNMMNGFINERKAGAQPDGVAA 279

Query: 274 GGEHQDLLSTLLS---TRDHAEDENEKLNDTEIKALLLNMFTAGTDTSASTVEWALAELI 333
           G    DLLS LL+        + + EK+ +T+IKALLLN+FTAGTDT++STVEWALAELI
Sbjct: 280 GEHGNDLLSVLLARMQEEQKLDGDGEKITETDIKALLLNLFTAGTDTTSSTVEWALAELI 339

Query: 334 RHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFRLHPPTPLSLPRMAAESC 393
           RHP ++ +AQ ELD+VVGR R+VS+ DLP+L YL AV+KETFRLHP TPLSLPR AAE C
Sbjct: 340 RHPDVLKEAQHELDTVVGRGRLVSESDLPRLPYLTAVIKETFRLHPSTPLSLPREAAEEC 399

Query: 394 EINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPDRFLPGGEKAHVDVKGNDFGVIPFG 453
           E++GY IPKG+TLLV+VWAI+RDP +W  PL+++P RFLPG   A VDVKG DFG+IPFG
Sbjct: 400 EVDGYRIPKGATLLVNVWAIARDPTQWPDPLQYQPSRFLPGRMHADVDVKGADFGLIPFG 459

Query: 454 AGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMDEAFGLTLRRAQPLVAHP 513
           AGRRIC G+S GLRMV ++TA+LVH FDW L +G +P +LNM+EA+GLTL+RA PL+  P
Sbjct: 460 AGRRICAGLSWGLRMVTLMTATLVHGFDWTLANGATPDKLNMEEAYGLTLQRAVPLMVQP 519

Query: 514 RPRLSPGVY 515
            PRL P  Y
Sbjct: 520 VPRLLPSAY 524

BLAST of CmaCh11G001980 vs. ExPASy Swiss-Prot
Match: A0A4D6Q414 (Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 GN=CYP75B138 PE=1 SV=1)

HSP 1 Score: 569.3 bits (1466), Expect = 4.4e-161
Identity = 284/523 (54.30%), Postives = 379/523 (72.47%), Query Frame = 0

Query: 3   EISLFFSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQLAA 62
           +I LF SA + L+  +  Y L  + + S  L LPPGPK +P +GNLPQ+G  P H  L A
Sbjct: 7   DIILFISAIVFLS--IYYYNLFSNAKRSNGLKLPPGPKGYPVLGNLPQLGAKP-HQALQA 66

Query: 63  MSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFA 122
            S  YGPLM LR GS+D+VVA+S ++A QFLK  D+NF +RPPN+GA+++A+NY D+VFA
Sbjct: 67  FSRVYGPLMRLRLGSVDLVVASSPSVAAQFLK-NDSNFCARPPNSGAEHMAFNYHDLVFA 126

Query: 123 PYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQAL---ARPGEAPATLGSLLTV 182
           PYGPRWRLLRK+SAVHL   KAL+DN+ VREEE+ VL + L   +R GE P  +G  + V
Sbjct: 127 PYGPRWRLLRKLSAVHLLGPKALDDNQNVREEELAVLARMLYERSRGGE-PVNVGKEMHV 186

Query: 183 CTANALGRTMIGRRVF---GDGSGGGD-----PMSDQFKAMVEQVMILSGKFNPGNFFPS 242
           C+ NAL R M+GRRVF     G GG +       +++FK MV +VM L+G FN G+F P 
Sbjct: 187 CSTNALSRAMMGRRVFEKLAVGGGGVEEEEEMKKAEEFKDMVVEVMTLAGVFNIGDFVPW 246

Query: 243 LEWLDLLGVAREMKKVHKWFDDLLSKILEEHRTLLASGGEHQDLLSTLLSTRDHAEDENE 302
           L+  D+ GV R+MK+VH+ ++  L K + E R+    G    DLLS L+  R  ++    
Sbjct: 247 LKPFDIQGVVRKMKRVHRRYNVFLDKFIAECRSSAKPGA--NDLLSVLIGQRGKSDGSGG 306

Query: 303 KLNDTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDL 362
           ++ DT IKAL+LN+ TAGTDTS+ST+EWAL ELIRHP ++ +AQQE+DS VGRDR+V++ 
Sbjct: 307 EITDTAIKALVLNLLTAGTDTSSSTIEWALTELIRHPDILKKAQQEIDSAVGRDRLVTES 366

Query: 363 DLPKLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKE 422
           D+PKL YLQA+VKE FR+HP TPLSLPRM+ E C+I GYHIPK STL V++WA+ RDP  
Sbjct: 367 DVPKLPYLQAIVKENFRMHPATPLSLPRMSIEECDIGGYHIPKNSTLFVNIWAMGRDPSI 426

Query: 423 WTCPLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHS 482
           W  P+EFRP RFLPGG+  H++V+GN F ++PFGAGRRIC G SMG+R+V    A+L+H+
Sbjct: 427 WPDPMEFRPSRFLPGGQGEHLEVRGNHFELMPFGAGRRICAGTSMGIRVVHSTVATLIHA 486

Query: 483 FDWALPSGLSPGELNMDEAFGLTLRRAQPLVAHPRPRLSPGVY 515
           FDW LP GL+  +++M+EAFG++L++A PL+AHP PRL+P  Y
Sbjct: 487 FDWKLPEGLTAEKIDMEEAFGISLQKAIPLMAHPIPRLAPKAY 522

BLAST of CmaCh11G001980 vs. TAIR 10
Match: AT5G07990.1 (Cytochrome P450 superfamily protein )

HSP 1 Score: 647.9 bits (1670), Expect = 6.9e-186
Identity = 318/506 (62.85%), Postives = 399/506 (78.85%), Query Frame = 0

Query: 12  ITLATVV-LLYRLLFHQRN-SRSLPLPPGPKPWPTVGNLPQIGGAPLHSQLAAMSGEYGP 71
           I LATV+ L+ R+  H+RN S +  LPPGP PWP +GNLP +G  P H  L+AM   YGP
Sbjct: 8   ILLATVLFLILRIFSHRRNRSHNNRLPPGPNPWPIIGNLPHMGTKP-HRTLSAMVTTYGP 67

Query: 72  LMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWR 131
           +++LR G +DVVVAAS ++AEQFLKI D NF SRPPN+GAK++AYNYQD+VFAPYG RWR
Sbjct: 68  ILHLRLGFVDVVVAASKSVAEQFLKIHDANFASRPPNSGAKHMAYNYQDLVFAPYGHRWR 127

Query: 132 LLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLGSLLTVCTANALGRTM 191
           LLRKIS+VHLFS+KALED + VR+EEVG L + L R G  P  LG L+ +C  NALGR M
Sbjct: 128 LLRKISSVHLFSAKALEDFKHVRQEEVGTLTRELVRVGTKPVNLGQLVNMCVVNALGREM 187

Query: 192 IGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGVAREMKKVHK 251
           IGRR+FG      D  +D+F++MV ++M L+G FN G+F PSL+WLDL GVA +MK++HK
Sbjct: 188 IGRRLFG---ADADHKADEFRSMVTEMMALAGVFNIGDFVPSLDWLDLQGVAGKMKRLHK 247

Query: 252 WFDDLLSKILEEHRTLLASGGEHQDLLSTLLSTR-DHAEDENEKLNDTEIKALLLNMFTA 311
            FD  LS IL+EH  +     +H D+LSTL+S +    + +   L DTEIKALLLNMFTA
Sbjct: 248 RFDAFLSSILKEHE-MNGQDQKHTDMLSTLISLKGTDLDGDGGSLTDTEIKALLLNMFTA 307

Query: 312 GTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFR 371
           GTDTSASTV+WA+AELIRHP +M +AQ+ELD VVGRDR V++ D+ +L YLQAV+KE FR
Sbjct: 308 GTDTSASTVDWAIAELIRHPDIMVKAQEELDIVVGRDRPVNESDIAQLPYLQAVIKENFR 367

Query: 372 LHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPDRFLPGGE 431
           LHPPTPLSLP +A+ESCEINGYHIPKGSTLL ++WAI+RDP +W+ PL F+P+RFLPGGE
Sbjct: 368 LHPPTPLSLPHIASESCEINGYHIPKGSTLLTNIWAIARDPDQWSDPLAFKPERFLPGGE 427

Query: 432 KAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMD 491
           K+ VDVKG+DF +IPFGAGRRIC G+S+GLR +Q LTA+LV  FDW L  G++P +LNM+
Sbjct: 428 KSGVDVKGSDFELIPFGAGRRICAGLSLGLRTIQFLTATLVQGFDWELAGGVTPEKLNME 487

Query: 492 EAFGLTLRRAQPLVAHPRPRLSPGVY 515
           E++GLTL+RA PLV HP+PRL+P VY
Sbjct: 488 ESYGLTLQRAVPLVVHPKPRLAPNVY 508

BLAST of CmaCh11G001980 vs. TAIR 10
Match: AT4G22710.1 (cytochrome P450, family 706, subfamily A, polypeptide 2 )

HSP 1 Score: 349.4 bits (895), Expect = 5.1e-96
Identity = 196/515 (38.06%), Postives = 292/515 (56.70%), Query Frame = 0

Query: 5   SLFFSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQLAAMS 64
           S+  + A  LA V  L+ +    ++ +  PLPPGP P P VGNLP +    LH+Q  A++
Sbjct: 24  SVMLAVAALLAVVCYLW-IQGKSKSKKEPPLPPGPWPLPIVGNLPFLNSDVLHTQFQALT 83

Query: 65  GEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPY 124
            +YGPLM +  GS   +V +S  +A + LK  D  F +       K   Y  +D++++PY
Sbjct: 84  LKYGPLMKIHLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWSPY 143

Query: 125 GPRWRLLRKISAVHLFSSKALEDNRCVREEEVG---VLVQALARPGEAPATLGSLLTVCT 184
           G  WR LRK+  + +F++ +LE +   R EE     V +  +AR G +P  LG  + +  
Sbjct: 144 GTHWRRLRKLCVMKMFTTPSLEASYSTRREETRQTIVHMSEMAREG-SPVNLGEQIFLSI 203

Query: 185 ANALGRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGVA 244
            N + R M G  V GD       + ++ K ++  +  + G  N  +FFP     D  G+ 
Sbjct: 204 FNVVTRMMWGATVEGDER---TSLGNELKTLISDISDIEGIQNYSDFFPLFSRFDFQGLV 263

Query: 245 REMKKVHKWFDDLLSKILEEHRTLLA-SGGEHQDLLSTLLSTRDHAEDENEKLNDTEIKA 304
           ++MK   K  D L  +++E H  ++     E +D L  LL  +D  +DE   L+ T +K+
Sbjct: 264 KQMKGHVKKLDLLFDRVMESHVKMVGKKSEEEEDFLQYLLRVKD--DDEKAPLSMTHVKS 323

Query: 305 LLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQ 364
           LL++M   G DTS +  E+A+AE++  P+++ + + ELD VVG+D +V +  LPKL YLQ
Sbjct: 324 LLMDMVLGGVDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEESHLPKLPYLQ 383

Query: 365 AVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRP 424
           AV+KET RLHP  PL +P   +E+  + GY +PK S + ++VWAI RDPK W  P EF+P
Sbjct: 384 AVMKETLRLHPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKNWDEPNEFKP 443

Query: 425 DRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGL 484
           +RFL       +D  G DF  +PFG+GRRIC  ++M  R+V    ASL+HSFDW  P G 
Sbjct: 444 ERFLENS----LDFNGGDFKYLPFGSGRRICAAINMAERLVLFNIASLLHSFDWKAPQG- 503

Query: 485 SPGELNMDEAFGLTLRRAQPLVAHPRPRLS-PGVY 515
              +  ++E FGL L+   PLVA P PRLS P +Y
Sbjct: 504 --QKFEVEEKFGLVLKLKSPLVAIPVPRLSDPKLY 524

BLAST of CmaCh11G001980 vs. TAIR 10
Match: AT4G22690.1 (cytochrome P450, family 706, subfamily A, polypeptide 1 )

HSP 1 Score: 346.3 bits (887), Expect = 4.3e-95
Identity = 192/506 (37.94%), Postives = 285/506 (56.32%), Query Frame = 0

Query: 14  LATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQLAAMSGEYGPLMYL 73
           L  VV  + +    ++    PLPPGP P P VGNLP +    LH+Q  A++ ++GPLM +
Sbjct: 63  LLAVVCYFWIQGKSKSKNGPPLPPGPWPLPIVGNLPFLNSDVLHTQFQALTLKHGPLMKI 122

Query: 74  RFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPRWRLLRK 133
             GS   +V +S  +A + LK  D  F +       K   Y  +D++++PYG  WR LRK
Sbjct: 123 HLGSKLAIVVSSPDMAREVLKTHDITFANHDLPEVGKINTYGGEDILWSPYGTHWRRLRK 182

Query: 134 ISAVHLFSSKALEDNRCVREEEVG---VLVQALARPGEAPATLGSLLTVCTANALGRTMI 193
           +  + +F++  LE +   R EE     V +  +AR G +P  LG  + +   N + R M 
Sbjct: 183 LCVMKMFTTPTLEASYSTRREETRQTIVHMSEMAREG-SPVNLGEQIFLSIFNVVTRMMW 242

Query: 194 GRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGVAREMKKVHKW 253
           G  V GD       + ++ K ++  +  + G  N  +FFP     D  G+ ++MK   K 
Sbjct: 243 GATVEGDER---TSLGNELKTLISDISDIEGIQNYSDFFPLFSRFDFQGLVKQMKGHVKK 302

Query: 254 FDDLLSKILEEHRTLLA-SGGEHQDLLSTLLSTRDHAEDENEKLNDTEIKALLLNMFTAG 313
            D L  +++E H  ++     E +D L  LL  +D  +DE   L+ T +K+LL++M   G
Sbjct: 303 LDLLFDRVMESHVKMVGKKSEEEEDFLQYLLRVKD--DDEKAPLSMTHVKSLLMDMVLGG 362

Query: 314 TDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAVVKETFRL 373
            DTS +  E+A+AE++  P+++ + + ELD VVG+D +V +  LPKL YLQAV+KET RL
Sbjct: 363 VDTSVNASEFAMAEIVSRPEVLNKIRLELDQVVGKDNIVEESHLPKLPYLQAVMKETLRL 422

Query: 374 HPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPDRFLPGGEK 433
           HP  PL +P   +E+  + GY +PK S + ++VWAI RDPK W  P EF+P+RFL     
Sbjct: 423 HPTLPLLVPHRNSETSVVAGYTVPKDSKIFINVWAIHRDPKNWDEPNEFKPERFLENS-- 482

Query: 434 AHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSPGELNMDE 493
             +D  G DF  +PFG+GRRIC  ++M  R+V    ASL+HSFDW  P G    +  ++E
Sbjct: 483 --LDFNGGDFKYLPFGSGRRICAAINMAERLVLFNIASLLHSFDWKAPQG---QKFEVEE 542

Query: 494 AFGLTLRRAQPLVAHPRPRLS-PGVY 515
            FGL L+   PLVA P PRLS P +Y
Sbjct: 543 KFGLVLKLKSPLVAIPVPRLSDPKLY 555

BLAST of CmaCh11G001980 vs. TAIR 10
Match: AT4G12310.1 (cytochrome P450, family 706, subfamily A, polypeptide 5 )

HSP 1 Score: 341.3 bits (874), Expect = 1.4e-93
Identity = 184/508 (36.22%), Postives = 290/508 (57.09%), Query Frame = 0

Query: 8   FSAAITLATVVLLYRLLFHQRNSRSLPLPPGPKPWPTVGNLPQIGGAPLHSQLAAMSGEY 67
           ++  I  AT  +L+ +    + S   PLPPGP+  P VGNLP +    LH+    ++  +
Sbjct: 20  YAVLILTATFSILWYIF---KRSPQPPLPPGPRGLPIVGNLPFL-DPDLHTYFTKLAQSH 79

Query: 68  GPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMVFAPYGPR 127
           GP+  L  GS   VV  S ++A + LK +D NF +      A+ + Y   D+V+ PYG  
Sbjct: 80  GPIFKLNLGSKLTVVVNSPSLASEILKDQDINFSNHDVPLTARAVTYGGLDLVWLPYGAE 139

Query: 128 WRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPG--EAPATLGSLLTVCTANAL 187
           WR+LRK+ A  LFS K L+    +R +E+    + L + G  ++P  +G  L +   N +
Sbjct: 140 WRMLRKVCAAKLFSRKTLDSFYELRRKEIRERTRCLYQKGLEKSPVNVGEQLFLTMMNLM 199

Query: 188 GRTMIGRRVFGDGSGGGDPMSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDLLGVAREMK 247
              + G  V  +     + +  +FK ++ ++  L G  N  +FFP L   DL G+ ++M 
Sbjct: 200 MNMLWGGSVKAEDM---ESVGTEFKGVISEITRLLGVPNVSDFFPMLARFDLQGLVKKMH 259

Query: 248 KVHKWFDDLLSKILEEHRTLLA---SGGEHQDLLSTLLSTRDHAEDENEKLNDTEIKALL 307
              +  D +L + +E+ + L +     GE +D L  L+  RD   D +  +    +KA+L
Sbjct: 260 LYARDLDAILDRAIEQMQRLRSRDGDDGECKDFLQHLMKLRDQEADSDVPITMNHVKAVL 319

Query: 308 LNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVSDLDLPKLGYLQAV 367
           ++M   GT++S +T+E+ +AELI +P++M +AQQELD VVG+D +V +  +  L Y+ AV
Sbjct: 320 MDMVVGGTESSTNTIEFVMAELISNPELMRRAQQELDEVVGKDNIVEESHITSLPYILAV 379

Query: 368 VKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDPKEWTCPLEFRPDR 427
           +KET RL+P  PL +P   +E+  + GY IPK + + ++VW+I RDP  W  P EFRP+R
Sbjct: 380 LKETLRLYPTIPLLVPHRPSETALVGGYTIPKNTKIFINVWSIQRDPNVWEYPTEFRPER 439

Query: 428 FLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLVHSFDWALPSGLSP 487
           FL   +K   D  G D+  +PFG+GRRIC G+++  RM+    A+L+HSFDW +P G   
Sbjct: 440 FL---DKKSCDFTGTDYSYLPFGSGRRICAGIALAERMILYTLATLLHSFDWTIPDG--- 499

Query: 488 GELNMDEAFGLTLRRAQPLVAHPRPRLS 511
             L+++E FG+ L+   PLVA P PRLS
Sbjct: 500 HVLDLEEKFGIVLKLKTPLVALPIPRLS 514

BLAST of CmaCh11G001980 vs. TAIR 10
Match: AT2G45570.1 (cytochrome P450, family 76, subfamily C, polypeptide 2 )

HSP 1 Score: 334.7 bits (857), Expect = 1.3e-91
Identity = 206/516 (39.92%), Postives = 294/516 (56.98%), Query Frame = 0

Query: 6   LFFSAAITLATVVLLYRLLF----HQRNSRS-LPLPPGPKPWPTVGNLPQIGGAPLHSQL 65
           +F  A   L   VL + ++F      R+SR  +P PPGP   P +GN+  +G  P HS  
Sbjct: 4   IFEQALFPLFCFVLSFFIIFFTTTRPRSSRKVVPSPPGPPRLPIIGNIHLVGRNPHHS-F 63

Query: 66  AAMSGEYGPLMYLRFGSIDVVVAASAAIAEQFLKIRDTNFLSRPPNAGAKYIAYNYQDMV 125
           A +S  YGP+M L+FGS++ VV  S   A + L+  D    SR P    + I ++   +V
Sbjct: 64  ADLSKTYGPIMSLKFGSLNTVVVTSPEAAREVLRTYDQILSSRTPTNSIRSINHDKVSVV 123

Query: 126 F-APYGPRWRLLRKISAVHLFSSKALEDNRCVREEEVGVLVQALARPGEAPATLG-SLLT 185
           +  P   RWRLLRK+SA  LFS + +E  + +RE +V  LV  ++   E    +  S  T
Sbjct: 124 WLPPSSSRWRLLRKLSATQLFSPQRIEATKTLRENKVKELVSFMSESSEREEAVDISRAT 183

Query: 186 VCTANALGRTMIGRRVFGDGSGGGDP-MSDQFKAMVEQVMILSGKFNPGNFFPSLEWLDL 245
             TA      +I   +F    G  D   S  F+  V  VM   G  +  NFFP L +LDL
Sbjct: 184 FITA----LNIISNILFSVDLGNYDSNKSGVFQDTVIGVMEAVGNPDAANFFPFLGFLDL 243

Query: 246 LGVAREMK-------KVHKWFDDLLSKILEEH-RTLLASGGEHQDLLSTLLSTRDHAEDE 305
            G  + +K       KV + F D  +K+ E+  R   +     +D +  LL   D  E +
Sbjct: 244 QGNRKTLKACSERLFKVFRGFID--AKLAEKSLRDTNSKDVRERDFVDVLL---DLTEGD 303

Query: 306 NEKLNDTEIKALLLNMFTAGTDTSASTVEWALAELIRHPKMMYQAQQELDSVVGRDRVVS 365
             +LN  +I  LLL++F AGTDT++STVEWA+AEL+R+P+ M +AQ E+D V+G+  VV 
Sbjct: 304 EAELNTNDIVHLLLDLFGAGTDTNSSTVEWAMAELLRNPETMVKAQAEIDCVIGQKGVVE 363

Query: 366 DLDLPKLGYLQAVVKETFRLHPPTPLSLPRMAAESCEINGYHIPKGSTLLVDVWAISRDP 425
           + D+  L YLQAVVKETFRLHP  PL +PR A    E+ G+ +PK + + V+VWAI RDP
Sbjct: 364 ESDISALPYLQAVVKETFRLHPAAPLLVPRKAESDVEVLGFMVPKDTQVFVNVWAIGRDP 423

Query: 426 KEWTCPLEFRPDRFLPGGEKAHVDVKGNDFGVIPFGAGRRICVGMSMGLRMVQMLTASLV 485
             W     F+P+RFL       +D++G D+ + PFGAGRRIC G+ + ++ V ++ ASL+
Sbjct: 424 NVWENSSRFKPERFL----GKDIDLRGRDYELTPFGAGRRICPGLPLAVKTVPLMLASLL 483

Query: 486 HSFDWALPSGLSPGELNMDEAFGLTLRRAQPLVAHP 506
           +SFDW LP+G+   +L+MDE FGLTL +  PL A P
Sbjct: 484 YSFDWKLPNGVGSEDLDMDETFGLTLHKTNPLHAVP 505

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9SBQ97.2e-18862.72Flavonoid 3'-monooxygenase OS=Petunia hybrida OX=4102 GN=CYP75B2 PE=2 SV=1[more]
Q9SD859.7e-18562.85Flavonoid 3'-monooxygenase OS=Arabidopsis thaliana OX=3702 GN=CYP75B1 PE=1 SV=1[more]
A0A4D6Q4153.3e-16957.70Flavonoid 3'-monooxygenase CYP75B137 OS=Crocosmia x crocosmiiflora OX=1053288 GN... [more]
Q7G6021.4e-16257.46Flavonoid 3'-monooxygenase CYP75B3 OS=Oryza sativa subsp. japonica OX=39947 GN=C... [more]
A0A4D6Q4144.4e-16154.30Flavonoid 3',5'-hydroxylase CYP75B138 OS=Crocosmia x crocosmiiflora OX=1053288 G... [more]
Match NameE-valueIdentityDescription
AT5G07990.16.9e-18662.85Cytochrome P450 superfamily protein [more]
AT4G22710.15.1e-9638.06cytochrome P450, family 706, subfamily A, polypeptide 2 [more]
AT4G22690.14.3e-9537.94cytochrome P450, family 706, subfamily A, polypeptide 1 [more]
AT4G12310.11.4e-9336.22cytochrome P450, family 706, subfamily A, polypeptide 5 [more]
AT2G45570.11.3e-9139.92cytochrome P450, family 76, subfamily C, polypeptide 2 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 276..296
NoneNo IPR availablePANTHERPTHR47956:SF3FLAVONOID 3'-MONOOXYGENASE CYP75B4coord: 10..507
NoneNo IPR availablePANTHERPTHR47956CYTOCHROME P450 71B11-RELATEDcoord: 10..507
IPR002401Cytochrome P450, E-class, group IPRINTSPR00463EP450Icoord: 297..314
score: 32.51
coord: 441..451
score: 57.81
coord: 64..83
score: 35.64
coord: 401..425
score: 37.95
coord: 317..343
score: 34.04
coord: 360..378
score: 40.21
coord: 451..474
score: 22.06
IPR001128Cytochrome P450PRINTSPR00385P450coord: 451..462
score: 23.55
coord: 361..372
score: 45.39
coord: 308..325
score: 38.69
coord: 442..451
score: 60.9
IPR001128Cytochrome P450PFAMPF00067p450coord: 36..489
e-value: 7.1E-102
score: 341.5
IPR036396Cytochrome P450 superfamilyGENE3D1.10.630.10Cytochrome P450coord: 28..510
e-value: 1.1E-123
score: 415.4
IPR036396Cytochrome P450 superfamilySUPERFAMILY48264Cytochrome P450coord: 36..508
IPR017972Cytochrome P450, conserved sitePROSITEPS00086CYTOCHROME_P450coord: 444..453

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G001980.1CmaCh11G001980.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
cellular_component GO:0016021 integral component of membrane
molecular_function GO:0020037 heme binding
molecular_function GO:0005506 iron ion binding
molecular_function GO:0016709 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen, NAD(P)H as one donor, and incorporation of one atom of oxygen
molecular_function GO:0004497 monooxygenase activity
molecular_function GO:0016705 oxidoreductase activity, acting on paired donors, with incorporation or reduction of molecular oxygen