CmaCh11G001620 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh11G001620
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionIsochorismate synthase
LocationCma_Chr11: 837375 .. 841723 (+)
RNA-Seq ExpressionCmaCh11G001620
SyntenyCmaCh11G001620
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
CCCAATCATGCTAGTTGAAGTGATGGGCATTTGAGAATGGCGAGTGGCGAGCGATAAGATTTGGTTCAGAGAAGTAAGCAAATAAAGAAAAGGGTAAGAATGAGCATTCCTAATAAGGCTATTTCTTCCACTTCTCAAACGTCACCCACCGGACCCTGTGTTGGGTTCGCCCAGCATCTGAGTCCGGGCCGCCGTATACCAACACGCGTAATTTGGCCAGGGCGGTGGCGGAGGAGATGGGGTGGGGTGGTTTGTCAGTCGCCGGCGATGGATGGGTGCAGAGCAGACCACGGAGGCGCCGTCGAGACGATGGAGACTCGAACTTTCCCGGCGGTTCCGACGGTGGGCTTGGCGACGGAGAATCTGAGGGGGTTGGTGGCAAAGTTGAAATCTGAGAAACCGGCGTTTAGTTCAGGAATTATTCGGCTCCAAGTAAGCCTCCTCCCAACTCTCCTGGACCTCTTTCAAATGCCTCGCATTACGCATTTCTTATCTCTTCCATGTACGTGGAATGAGAACCGCCACGTGCGGTAATTTTTTCTTTTATTATTGTTATTATTATTATTATCTTTTTAACATTTAATCATACACCCATTTCTTATCTCTCCTCTGTTTGGCATCTGGGATTATCCTAAACCGGAATAGACCTTTTCCTCCAAAATAAATAAATAAATAAATACCCATTTTCTTTTTTAATAATTATCAACTAAACCTAATAATTAGTAAGAAGAAAAGAAAATCTAACAAATAAAGTAATAAAGTAATAAAATTGAGTTGAAATTCTGTTGACTTTGTGCACATTCTGAGACTGAACGAGGGTTGACTATTTTGCAGCTTCCAATCCAACAGCACATTAAGGCGATTCATTGGCTTTGTTCTCAGCCTCTGCTTCTTCCTCGTATCTTCTTCTCCGGCCGGAGCAGAGCCATTGATTCTGATCCTCTCCCCTCTGTCAGCTCCAATGCCCACCACCGCCTCGCCAGCGTCGCAGGTGTGGGCTCTGCAGTCGTCTTCTGCCGCCATCATCAGTTTTCGAACTCTGATTGGGCGTCTATAAAGAGGTAAACTCATTTCGCCTCTATTTATATTCACTCCGCCATCTGTTCGATGAAATGCCTGGGCTAATATTCTGTAAATTATAGGTTCCTCTCAAAGAAATGCCCCCTGATTCGTGCTTTTGGAGGTATTCGCTTTGATTCACGTGCGAACATATCGCCTGAATGGGAGCCTTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTACAGACTTCTTTTTCCAATCTCGTTTATCGCAATTGGATAATGGACGCCAAAATCACAGGTTGAGTTTGATGAGTTCGAGGATGACTCGATTCTTGCCGCTACTGTAGCTTGGGATCATGCCTGTTCTTGGACTTGGGAAAATGCTGTTGAATCCCTTCAATCCACAATCGAACAGGTCGACTATTTGATTCACTTTGATTCTCTTTGTTCATTGCATTGAAAGGAAAGATATTCTCCCATAGATAATCACTCCCTAGTAAATTTCTTATCATTCAGGTTTCTTCTGATAATGTAAATGTACAAAGAGATCTTCCTCAGATATCCATACTTAGCCACAATCATGTTCCCACCAAAACATTCTGGGATCGTGCAATTAACAGGGCGCTGCAAGCGATTGACCAATGCGACTCAGATCTTACCAAGGCACTCACCAATTCTTCACTCTTACCAAAAATTTATTTTCGTTTCTATTCATCAGTAAAATCGGCTGTCTAATCTTATGCATTATATTTAGGTCGTACTTGCACGCAGTACCCGAATAGTGACAAGCGTAAATGTTGATCCTGTGACATGGGTTGCATGTTTACAGGTAGCTATTGCATGGGTTGCATGTTTATGCAGTAATTTTGTTTCCTGAGTCTAGTTTTGATCGTATCAGAAAGAGGGGGAAAGCTCTTATCAGTTTTGTGTCCAGCCAGCCAATGCTCCTGCCTTCATAGGAAATACGGTATGTAACAAAATTTCATACGCTTCCAGTCAATCATTTCATTTTGTTGCCTAATTTCAAAATGGGGAATATCTGTGATCCGGCTGAAGATGGTACTCATTAAAGATGTTTTCAATTAGTTTCCTACCCGTTTGGGTTTTGTAATATTTATTTTCTTACTAACTTTGGCTTTGATATGTTCGTAATAGCCTGAGCGGCTATTCCATAGAGAAGGACTTAGTATTATATGTGAGGCCTTAGCTGCAACCCGTGCTAGAGGCAAATCGGCTATTGAAGACCTTAAAATAGAACAAGATTTATTATCCAAGTTGGTCTCATCTCTAAATTCTTATTTTTTCTACTCAATGATGCTGTAACCCTGTTGCCTCAACCAATGAGCTGCGGGCGGGAGTTTTCACTAACTCTACTTTCTATGTGCAGCCCAAAGGATGACCTTGAGTTTTCGATTGTGAGGGAAGCCATCAAAAGGAAGTTAGAGGCAAGTTTGAGCTTCAGTCTTGCTCTTTTGCTATCTTGCTTTCTGTCGATGAAATGAAACTTCCCCTTAATCGAGGCTCGACTCCTTCTCTAGAGCCCTCGAACAAAGCACACTCTTTATTCAACACTTGAGTCACTTTTGACTCACCTTCGAGGTTCACAATTTTTTTGTTTGACACTTAAGGATTTTGTAGAAATGACTAAGTTAGGAACCACGTACCTCTCCACAATGGTATGATATTATCTACTTTGAGCATAAGCTCTCGTGACTTTGATTTTGGTTTACCCAAAAGGCCTCATACCAATGGAGATGGTATTCCTTGCTTATAAATTCATAATCTTCTCCTTAATTGGCCAATGTGGGACTCCTCTCCATATAAGTTTGGTCTGTGGCTGCTATGAGCTATGAAAAATGATATTTGCAGTGCTTACAAACCGTGAATTTCTTCTATTTTTTGTCATCGTTTTTCAAGCACCAAACAACATTTACATAGGTTTCTCAATAAGATGCTGAAATTTATAGGCAATTACGAAGAGGACAACATTCTGAGAATATTGCAGATTCTTAGCCAGCTTAATCCTGTTTGTAGGCTGTATGTGATGCTGTAGTCGTTGAACCACATAAATCAGTTAGAAAACTTCCCAGAATTCAACATCTGTATGCCCAGCTAGCAGGCAGATTGAGAACCAAGGATGATGAGGTAAGAATCTCCAAATATTTATGTCCCTGTAAACTAAACTTCCCTCACGTGAATATACCGCTCTAACTCTACTTTGTTGCTTGAAAAGTTTGAAATTTTATCAACGCTTCATCCAACTCCCGCGGTTTGTGGATTTCCAACAGAGGAAGCTCGAGATTTAATTGCAGAAACTGGTAAGGTTCATGAGTGCGTGACCCTTTCTATTAGCTCCCCAAGCCAATCCAAGTTATGGATGGTTTCCTTCAAAATGCTGATTATGGGTCTTGTAATTCTGTGGTCTGCCAAATCTAAGTCGTCTTCATCCTTCTGTATACATTTAAGAACTTGGGATATTTATGCAGAGTTATTTGATAGAGGGATGTACGCCGGGCCTGTTGGTTGGTTTGGAGGAGAAGAGAGTGAGTTTGCTGTGGGCATCAGATCAGCATTAGTGGAGACAGTAAGATGGCGTCTACCATATGAGCTACAAACTTAAATACTTCCATTGAATCCTTACTCGAATTTAAAATTTCTAGAGCTTAGACATCATTTCCATTACAATTCTCTGAGTTCTTACAGTCTTTGATTATAGGGTCATGGTGCCTTGGTCTATGCGGGAACAGGGATAGTAAAAGGAAGTAATCCGTCTTTGGAGTGGGATGAATTGGAGCTCAAGATATCTCAGGTACTCTGGAACTTGCATCTTTTGTCTCGAAAATGCTCTACTTAGTAGCTCAATTTTCATACCTTATACCTTTGATTTGCATATTTGCAGGTTACCAGATCGCTTAAACTGGAATTGGCTTGAGCACCCACGTTTCATTGGTGAAGCTGTAGTCTGGGAAAGTAAAGGCCTAAGCTTTTCGATCAGGTTCCTTCCGGTTGTGGCTTGAGTCAGCAAATGGTTAGTTCATTCTTCGTTTTGCTTTGCCTGTTTAGATCACAGTGCCGCATTCATAACGTATATAGATAGGTGGCTGCTTCATTTAAGTATGATCGAATGTTTTAGAATCAATTACTTGTAGAGAAATATATTTGTACATTTATTGGAGAAACCACTAGTATTTAGAGTATTAAGAACAATTTCCAAAGCTCAAATGTAAATTACCAAACATATATGACACTTGAAAACAGAAAACCACAACACACACTAAATACCATCAACTTGGTGAAATAGAGTTTTGAACTAGTGAGAAGAAGAA

mRNA sequence

CCCAATCATGCTAGTTGAAGTGATGGGCATTTGAGAATGGCGAGTGGCGAGCGATAAGATTTGGTTCAGAGAAGTAAGCAAATAAAGAAAAGGGTAAGAATGAGCATTCCTAATAAGGCTATTTCTTCCACTTCTCAAACGTCACCCACCGGACCCTGTGTTGGGTTCGCCCAGCATCTGAGTCCGGGCCGCCGTATACCAACACGCGTAATTTGGCCAGGGCGGTGGCGGAGGAGATGGGGTGGGGTGGTTTGTCAGTCGCCGGCGATGGATGGGTGCAGAGCAGACCACGGAGGCGCCGTCGAGACGATGGAGACTCGAACTTTCCCGGCGGTTCCGACGGTGGGCTTGGCGACGGAGAATCTGAGGGGGTTGGTGGCAAAGTTGAAATCTGAGAAACCGGCGTTTAGTTCAGGAATTATTCGGCTCCAACTTCCAATCCAACAGCACATTAAGGCGATTCATTGGCTTTGTTCTCAGCCTCTGCTTCTTCCTCGTATCTTCTTCTCCGGCCGGAGCAGAGCCATTGATTCTGATCCTCTCCCCTCTGTCAGCTCCAATGCCCACCACCGCCTCGCCAGCGTCGCAGGTGTGGGCTCTGCAGTCGTCTTCTGCCGCCATCATCAGTTTTCGAACTCTGATTGGGCGTCTATAAAGAGGTTCCTCTCAAAGAAATGCCCCCTGATTCGTGCTTTTGGAGGTATTCGCTTTGATTCACGTGCGAACATATCGCCTGAATGGGAGCCTTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTTGAGTTTGATGAGTTCGAGGATGACTCGATTCTTGCCGCTACTGTAGCTTGGGATCATGCCTGTTCTTGGACTTGGGAAAATGCTGTTGAATCCCTTCAATCCACAATCGAACAGGTTTCTTCTGATAATGTAAATGTACAAAGAGATCTTCCTCAGATATCCATACTTAGCCACAATCATGTTCCCACCAAAACATTCTGGGATCGTGCAATTAACAGGGCGCTGCAAGCGATTGACCAATGCGACTCAGATCTTACCAAGGTCGTACTTGCACGCAGTACCCGAATAGTGACAAGCGTAAATGTTGATCCTGTGACATGGGTTGCATGTTTACAGAAAGAGGGGGAAAGCTCTTATCAGTTTTGTGTCCAGCCAGCCAATGCTCCTGCCTTCATAGGAAATACGCCTGAGCGGCTATTCCATAGAGAAGGACTTAGTATTATATGTGAGGCCTTAGCTGCAACCCGTGCTAGAGGCAAATCGGCTATTGAAGACCTTAAAATAGAACAAGATTTATTATCCAACCCAAAGGATGACCTTGAGTTTTCGATTGTGAGGGAAGCCATCAAAAGGAAGTTAGAGGCTGTATGTGATGCTGTAGTCGTTGAACCACATAAATCAGTTAGAAAACTTCCCAGAATTCAACATCTGTATGCCCAGCTAGCAGGCAGATTGAGAACCAAGGATGATGAGTTTGAAATTTTATCAACGCTTCATCCAACTCCCGCGGTTTGTGGATTTCCAACAGAGGAAGCTCGAGATTTAATTGCAGAAACTGAGTTATTTGATAGAGGGATGTACGCCGGGCCTGTTGGTTGGTTTGGAGGAGAAGAGAGTGAGTTTGCTGTGGGCATCAGATCAGCATTAGTGGAGACAGGTCATGGTGCCTTGGTCTATGCGGGAACAGGGATAGTAAAAGGAAGTAATCCGTCTTTGGAGTGGGATGAATTGGAGCTCAAGATATCTCAGGTTACCAGATCGCTTAAACTGGAATTGGCTTGAGCACCCACGTTTCATTGGTGAAGCTGTAGTCTGGGAAAGTAAAGGCCTAAGCTTTTCGATCAGGTTCCTTCCGGTTGTGGCTTGAGTCAGCAAATGGTTAGTTCATTCTTCGTTTTGCTTTGCCTGTTTAGATCACAGTGCCGCATTCATAACGTATATAGATAGGTGGCTGCTTCATTTAAGTATGATCGAATGTTTTAGAATCAATTACTTGTAGAGAAATATATTTGTACATTTATTGGAGAAACCACTAGTATTTAGAGTATTAAGAACAATTTCCAAAGCTCAAATGTAAATTACCAAACATATATGACACTTGAAAACAGAAAACCACAACACACACTAAATACCATCAACTTGGTGAAATAGAGTTTTGAACTAGTGAGAAGAAGAA

Coding sequence (CDS)

ATGAGCATTCCTAATAAGGCTATTTCTTCCACTTCTCAAACGTCACCCACCGGACCCTGTGTTGGGTTCGCCCAGCATCTGAGTCCGGGCCGCCGTATACCAACACGCGTAATTTGGCCAGGGCGGTGGCGGAGGAGATGGGGTGGGGTGGTTTGTCAGTCGCCGGCGATGGATGGGTGCAGAGCAGACCACGGAGGCGCCGTCGAGACGATGGAGACTCGAACTTTCCCGGCGGTTCCGACGGTGGGCTTGGCGACGGAGAATCTGAGGGGGTTGGTGGCAAAGTTGAAATCTGAGAAACCGGCGTTTAGTTCAGGAATTATTCGGCTCCAACTTCCAATCCAACAGCACATTAAGGCGATTCATTGGCTTTGTTCTCAGCCTCTGCTTCTTCCTCGTATCTTCTTCTCCGGCCGGAGCAGAGCCATTGATTCTGATCCTCTCCCCTCTGTCAGCTCCAATGCCCACCACCGCCTCGCCAGCGTCGCAGGTGTGGGCTCTGCAGTCGTCTTCTGCCGCCATCATCAGTTTTCGAACTCTGATTGGGCGTCTATAAAGAGGTTCCTCTCAAAGAAATGCCCCCTGATTCGTGCTTTTGGAGGTATTCGCTTTGATTCACGTGCGAACATATCGCCTGAATGGGAGCCTTTTGGCTCGTTTTACTTCATGGTCCCTCAGGTTGAGTTTGATGAGTTCGAGGATGACTCGATTCTTGCCGCTACTGTAGCTTGGGATCATGCCTGTTCTTGGACTTGGGAAAATGCTGTTGAATCCCTTCAATCCACAATCGAACAGGTTTCTTCTGATAATGTAAATGTACAAAGAGATCTTCCTCAGATATCCATACTTAGCCACAATCATGTTCCCACCAAAACATTCTGGGATCGTGCAATTAACAGGGCGCTGCAAGCGATTGACCAATGCGACTCAGATCTTACCAAGGTCGTACTTGCACGCAGTACCCGAATAGTGACAAGCGTAAATGTTGATCCTGTGACATGGGTTGCATGTTTACAGAAAGAGGGGGAAAGCTCTTATCAGTTTTGTGTCCAGCCAGCCAATGCTCCTGCCTTCATAGGAAATACGCCTGAGCGGCTATTCCATAGAGAAGGACTTAGTATTATATGTGAGGCCTTAGCTGCAACCCGTGCTAGAGGCAAATCGGCTATTGAAGACCTTAAAATAGAACAAGATTTATTATCCAACCCAAAGGATGACCTTGAGTTTTCGATTGTGAGGGAAGCCATCAAAAGGAAGTTAGAGGCTGTATGTGATGCTGTAGTCGTTGAACCACATAAATCAGTTAGAAAACTTCCCAGAATTCAACATCTGTATGCCCAGCTAGCAGGCAGATTGAGAACCAAGGATGATGAGTTTGAAATTTTATCAACGCTTCATCCAACTCCCGCGGTTTGTGGATTTCCAACAGAGGAAGCTCGAGATTTAATTGCAGAAACTGAGTTATTTGATAGAGGGATGTACGCCGGGCCTGTTGGTTGGTTTGGAGGAGAAGAGAGTGAGTTTGCTGTGGGCATCAGATCAGCATTAGTGGAGACAGGTCATGGTGCCTTGGTCTATGCGGGAACAGGGATAGTAAAAGGAAGTAATCCGTCTTTGGAGTGGGATGAATTGGAGCTCAAGATATCTCAGGTTACCAGATCGCTTAAACTGGAATTGGCTTGA

Protein sequence

MSIPNKAISSTSQTSPTGPCVGFAQHLSPGRRIPTRVIWPGRWRRRWGGVVCQSPAMDGCRADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQHIKAIHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSSNAHHRLASVAGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTFWDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPANAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLELA
Homology
BLAST of CmaCh11G001620 vs. ExPASy Swiss-Prot
Match: Q9M9V6 (Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 PE=2 SV=2)

HSP 1 Score: 608.6 bits (1568), Expect = 7.1e-173
Identity = 310/508 (61.02%), Postives = 385/508 (75.79%), Query Frame = 0

Query: 56  AMDGCRADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQ 115
           +M+GC ADH   + T+ETRT   VP+   ATE L   V+ LKS+ P FSSGI+RLQ+PI+
Sbjct: 50  SMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIE 109

Query: 116 QHIKAIHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSS---NAHHRLASVAGVGSAVVFC 175
           Q I AI WL +Q  +LPR FFS RS +   D L   SS   ++ H   SVAG+GSAV F 
Sbjct: 110 QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFR 169

Query: 176 RHHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEF 235
               FS+ DW SI+RFLS K PLIRA+GG+RFD    I+ EWE FGSFYF VPQVEFDEF
Sbjct: 170 DLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEF 229

Query: 236 EDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKT 295
              S+LAATVAWD+  SWT ENA+E+LQ T+ QVSS  + ++R+   + ++S NHVP++ 
Sbjct: 230 GGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVPSEG 289

Query: 296 FWDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQP 355
            +  A+N AL+ I    S L+KVVLARS+RI+T  ++DP+ W+A LQ EG+ +YQFC+QP
Sbjct: 290 AYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQFCLQP 349

Query: 356 ANAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIV 415
             APAFIGNTPERLFHR+ L +  EALAATR RG S + +++IE+DLL++PKDDLEFSIV
Sbjct: 350 PGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDLEFSIV 409

Query: 416 REAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVC 475
           RE I+ KL+ +CD VVV+PHKSVRKL R+QHLY+QLAG+L+ +DDEF IL+ LHPTPAVC
Sbjct: 410 RENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHPTPAVC 469

Query: 476 GFPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIV 535
           G P EEAR LI + E FDRGMYAGP+G+FGG ESEF+VGIRSALVE G GAL+YAGTGIV
Sbjct: 470 GCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYAGTGIV 529

Query: 536 KGSNPSLEWDELELKISQVTRSLKLELA 561
            GSNPS EW+ELELKISQ T+SL+ E A
Sbjct: 530 SGSNPSSEWNELELKISQFTKSLEHESA 557

BLAST of CmaCh11G001620 vs. ExPASy Swiss-Prot
Match: Q9S7H8 (Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 PE=1 SV=2)

HSP 1 Score: 586.6 bits (1511), Expect = 2.9e-166
Identity = 300/516 (58.14%), Postives = 378/516 (73.26%), Query Frame = 0

Query: 54  SPAMDGCRADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLP 113
           S +M+GC  D    + T+ETRT  AV +   ATE L   V++LKS+ P+FSSG++RLQ+P
Sbjct: 45  SMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVP 104

Query: 114 IQQHIKAIHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSS-----------NAHHRLASV 173
           I Q I AI WL +Q  + PR FFS RS     D L  +++           ++   L SV
Sbjct: 105 IDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSV 164

Query: 174 AGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYF 233
           AG+GSAV F     FS+ DW SI+RFLS   PLIRA+GG+RFD    I+ EWEPFG+FYF
Sbjct: 165 AGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYF 224

Query: 234 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISI 293
            VPQVEF+EF   S+LAAT+AWD   SWT ENA+E+LQ T+ QVSS  + ++     +S+
Sbjct: 225 SVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSV 284

Query: 294 LSHNHVPTKTFWDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEG 353
           LS NHVPTK  +  A+ +AL+ I+Q  S L KVVLAR++RI+T  ++DP+ W+A LQ+EG
Sbjct: 285 LSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREG 344

Query: 354 ESSYQFCVQPANAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSN 413
             +YQFC+QP  APAFIGNTPERLF R  L +  EALAATR R  S+  D++IE+DLL++
Sbjct: 345 HDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTS 404

Query: 414 PKDDLEFSIVREAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEIL 473
           PKDDLEFSIVRE I+ KL  +CD VVV+P K+VRKL R+QHLY+QLAGRL  +DDE++IL
Sbjct: 405 PKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKIL 464

Query: 474 STLHPTPAVCGFPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHG 533
           + LHPTPAVCG P EEAR LI E E FDRGMYAGP+G+FGGEESEFAVGIRSALVE G G
Sbjct: 465 AALHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLG 524

Query: 534 ALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLE 559
           AL+YAGTGIV GS+PS EW+EL+LKISQ T+S++ E
Sbjct: 525 ALIYAGTGIVAGSDPSSEWNELDLKISQFTKSIEYE 560

BLAST of CmaCh11G001620 vs. ExPASy Swiss-Prot
Match: Q9ZPC0 (Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1)

HSP 1 Score: 577.4 bits (1487), Expect = 1.7e-163
Identity = 293/524 (55.92%), Postives = 388/524 (74.05%), Query Frame = 0

Query: 54  SPAMDGCRADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLP 113
           S +M+GC  D    V T+ETRT PAV T  LA E L   VA LKS  P+  SGIIRL++P
Sbjct: 52  SLSMNGCNGDPRAPVGTIETRTLPAVSTPALAMERLSSAVANLKSTLPSAQSGIIRLEVP 111

Query: 114 IQQHIKAIHWLCSQPL--LLPRIFFSGRSRAIDSD----------------PLPSVSSNA 173
           I++HI+A+ WL SQ    LLPR +FSGRS+   SD                 L  +S+++
Sbjct: 112 IEEHIEALDWLHSQDQKNLLPRCYFSGRSQVTFSDFTSNDLTNRNGSAANGHLQRISTSS 171

Query: 174 HHR-LASVAGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEW 233
             + L SVAGVGSAV+F   + FS  DW SIKRFLSK CPLIRA+G IRFD+R +I+PEW
Sbjct: 172 DDKNLVSVAGVGSAVLFRSPNPFSFDDWLSIKRFLSKNCPLIRAYGAIRFDARPHIAPEW 231

Query: 234 EPFGSFYFMVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQ 293
           + FGSFYF+VPQVEFDE    S++AATVAWD+A S T++ A+ +LQ+T+EQVSS    ++
Sbjct: 232 KAFGSFYFVVPQVEFDELHGSSMIAATVAWDNALSLTYQQAIVALQTTMEQVSSTVSKLR 291

Query: 294 RDLPQISILSHNHVPTKTFWDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTW 353
           +D+   S++S  ++P +T WD  +NR L+ I    S LTKVVLAR ++++T+ ++DP+ W
Sbjct: 292 QDVSHTSLVSKANIPDRTSWDLTLNRVLEEIGNKYSPLTKVVLARRSQVITTSDIDPLAW 351

Query: 354 VACLQKEGESSYQFCVQPANAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLK 413
           ++  + +G+ +YQFC+QP  APAFIGNTPE+LF R+ L++  EALAATRARG+S   DL+
Sbjct: 352 LSSFKADGKDAYQFCLQPHEAPAFIGNTPEQLFGRDQLTVFSEALAATRARGESDSLDLQ 411

Query: 414 IEQDLLSNPKDDLEFSIVREAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRT 473
           +  DL S+PKD+ EF+IVRE I++KL+A+C +V  EP KSVRKL RIQHLYA+ AGRLR+
Sbjct: 412 MAHDLFSSPKDNHEFAIVRENIRQKLDAICTSVETEPMKSVRKLKRIQHLYARFAGRLRS 471

Query: 474 KDDEFEILSTLHPTPAVCGFPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRS 533
           +DDEF+ILS+LHPTPAVCGFP E+AR  IAE E+FDRG+YAGPVG+FGG +S+F+VGIRS
Sbjct: 472 EDDEFKILSSLHPTPAVCGFPMEDARKFIAENEMFDRGLYAGPVGFFGGAQSDFSVGIRS 531

Query: 534 ALVETGHGALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLE 559
           AL+    GAL+YAG G+V+GS+P+LEW ELELK SQ  + +KLE
Sbjct: 532 ALIGKDAGALIYAGLGVVEGSDPALEWQELELKASQFMKLMKLE 575

BLAST of CmaCh11G001620 vs. ExPASy Swiss-Prot
Match: P23973 (Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF PE=3 SV=2)

HSP 1 Score: 158.3 bits (399), Expect = 2.5e-37
Identity = 111/363 (30.58%), Postives = 179/363 (49.31%), Query Frame = 0

Query: 199 FGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFEDDSILAATVAWDHACSWTWENAVES 258
           FGG  FD       +W+ F    F VP +         +L  T          W +  E 
Sbjct: 119 FGGFSFDPCEERGSQWDHFSEGDFFVPAL---------MLTMTAEGPFLTVNRWVSGGED 178

Query: 259 LQSTIEQVSSDNVN-VQRDLPQ--ISILSHNHVPTKTFWDRAINRALQAIDQCDSDLTKV 318
            ++ +E + +     +  D  Q   ++++      K  W +AI  A   I +   D  KV
Sbjct: 179 AEAVLEGLKAFAAEFMVPDFKQEDQAVIAAAEELDKDDWLKAIETATSQIKEKQYD--KV 238

Query: 319 VLARSTRIVTS--VNVDPVTWVACLQKEGESSYQFCVQPANAPAFIGNTPERLFHREGLS 378
           VLAR   +     + ++PV  +  L  + ++SY F ++      F+G +PERL  R+G +
Sbjct: 239 VLARELLLTFDGPIQIEPV--LKTLLDDQQTSYVFAIE-QEGKTFVGASPERLIKRDGGT 298

Query: 379 IICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVREAIKRKLEAVCDAVVVEPHK 438
           ++   LA +  RG +  +D +I  +LL++ K+ LE  IV   I     + C  V      
Sbjct: 299 VMSSCLAGSIKRGVNEEDDRRIGLELLNDEKNLLEHDIVVGMIHNAFVSSCSEVEKPDGP 358

Query: 439 SVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCGFPTEEARDLIAETELFDRGM 498
            + K   +QHL+  + G+LR     F+++  LHPTPA+ G P E+A D+I E E   RG 
Sbjct: 359 VLYKTKSVQHLFTPIVGQLRESASIFDLIEKLHPTPALGGSPQEKAVDVIREIEPMSRGW 418

Query: 499 YAGPVGWFGGEES-EFAVGIRSALVETGHGALVYAGTGIVKGSNPSLEWDELELKISQVT 556
           YA P+GW   +++ EFAV IRS L+E G  A ++AG GIV+ S P  E++E ++K+  + 
Sbjct: 419 YAAPIGWIDSQDNGEFAVAIRSGLIE-GSTARLFAGCGIVEDSEPISEYEETQIKLKPMI 466

BLAST of CmaCh11G001620 vs. ExPASy Swiss-Prot
Match: Q51508 (Salicylate biosynthesis isochorismate synthase OS=Pseudomonas aeruginosa (strain ATCC 15692 / DSM 22644 / CIP 104116 / JCM 14847 / LMG 12228 / 1C / PRS 101 / PAO1) OX=208964 GN=pchA PE=1 SV=1)

HSP 1 Score: 148.3 bits (373), Expect = 2.6e-34
Identity = 95/263 (36.12%), Postives = 140/263 (53.23%), Query Frame = 0

Query: 294 WDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPA 353
           W+  ++ A+ ++ Q      KVVLAR T+     +++P   +  L+ +   +  F  +  
Sbjct: 203 WEAKVSDAVSSVRQ--GRFGKVVLAR-TQARPLGDIEPWQVIEHLRLQHADAQLFACRRG 262

Query: 354 NAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVR 413
           NA  F+G +PERL        +  ALA T ARG  A ED ++ Q LL + KD  E  +V 
Sbjct: 263 NA-CFLGASPERLVRIRAGEALTHALAGTIARGGDAQEDARLGQALLDSAKDRHEHQLVV 322

Query: 414 EAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCG 473
           EAI+  LE   + + +     +++L R+QHL   +  RL        +L  LHPTPAV G
Sbjct: 323 EAIRTALEPFSEVLEIPDAPGLKRLARVQHLNTPIRARLADAGGILRLLQALHPTPAVGG 382

Query: 474 FPTEEARDLIAETELFDRGMYAGPVGWFGGE-ESEFAVGIRSALVETGHGALVYAGTGIV 533
           +P   A D I + E  DRG YA P+GW  GE   +F V +RSAL+  G G L +AG G+V
Sbjct: 383 YPRSAALDYIRQHEGMDRGWYAAPLGWLDGEGNGDFLVALRSALLTPGRGYL-FAGCGLV 442

Query: 534 KGSNPSLEWDELELKISQVTRSL 556
             S P+ E+ E  LK+S +  +L
Sbjct: 443 GDSEPAHEYRETCLKLSAMREAL 460

BLAST of CmaCh11G001620 vs. TAIR 10
Match: AT1G18870.1 (isochorismate synthase 2 )

HSP 1 Score: 608.6 bits (1568), Expect = 5.0e-174
Identity = 310/508 (61.02%), Postives = 385/508 (75.79%), Query Frame = 0

Query: 56  AMDGCRADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQ 115
           +M+GC ADH   + T+ETRT   VP+   ATE L   V+ LKS+ P FSSGI+RLQ+PI+
Sbjct: 50  SMNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIE 109

Query: 116 QHIKAIHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSS---NAHHRLASVAGVGSAVVFC 175
           Q I AI WL +Q  +LPR FFS RS +   D L   SS   ++ H   SVAG+GSAV F 
Sbjct: 110 QKIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFR 169

Query: 176 RHHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEF 235
               FS+ DW SI+RFLS K PLIRA+GG+RFD    I+ EWE FGSFYF VPQVEFDEF
Sbjct: 170 DLDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEF 229

Query: 236 EDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKT 295
              S+LAATVAWD+  SWT ENA+E+LQ T+ QVSS  + ++R+   + ++S NHVP++ 
Sbjct: 230 GGSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVPSEG 289

Query: 296 FWDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQP 355
            +  A+N AL+ I    S L+KVVLARS+RI+T  ++DP+ W+A LQ EG+ +YQFC+QP
Sbjct: 290 AYYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQFCLQP 349

Query: 356 ANAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIV 415
             APAFIGNTPERLFHR+ L +  EALAATR RG S + +++IE+DLL++PKDDLEFSIV
Sbjct: 350 PGAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDLEFSIV 409

Query: 416 REAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVC 475
           RE I+ KL+ +CD VVV+PHKSVRKL R+QHLY+QLAG+L+ +DDEF IL+ LHPTPAVC
Sbjct: 410 RENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHPTPAVC 469

Query: 476 GFPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIV 535
           G P EEAR LI + E FDRGMYAGP+G+FGG ESEF+VGIRSALVE G GAL+YAGTGIV
Sbjct: 470 GCPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYAGTGIV 529

Query: 536 KGSNPSLEWDELELKISQVTRSLKLELA 561
            GSNPS EW+ELELKISQ T+SL+ E A
Sbjct: 530 SGSNPSSEWNELELKISQFTKSLEHESA 557

BLAST of CmaCh11G001620 vs. TAIR 10
Match: AT1G18870.2 (isochorismate synthase 2 )

HSP 1 Score: 608.2 bits (1567), Expect = 6.6e-174
Identity = 310/507 (61.14%), Postives = 384/507 (75.74%), Query Frame = 0

Query: 57  MDGCRADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLPIQQ 116
           M+GC ADH   + T+ETRT   VP+   ATE L   V+ LKS+ P FSSGI+RLQ+PI+Q
Sbjct: 1   MNGCEADHKAPLGTVETRTLSTVPSPAAATERLITAVSDLKSQPPPFSSGIVRLQVPIEQ 60

Query: 117 HIKAIHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSS---NAHHRLASVAGVGSAVVFCR 176
            I AI WL +Q  +LPR FFS RS +   D L   SS   ++ H   SVAG+GSAV F  
Sbjct: 61  KIGAIDWLHAQNEILPRSFFSRRSDSGRPDLLQDFSSDNGSSDHNPVSVAGIGSAVFFRD 120

Query: 177 HHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYFMVPQVEFDEFE 236
              FS+ DW SI+RFLS K PLIRA+GG+RFD    I+ EWE FGSFYF VPQVEFDEF 
Sbjct: 121 LDPFSHDDWRSIRRFLSSKSPLIRAYGGLRFDPTGKIAVEWEHFGSFYFTVPQVEFDEFG 180

Query: 237 DDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISILSHNHVPTKTF 296
             S+LAATVAWD+  SWT ENA+E+LQ T+ QVSS  + ++R+   + ++S NHVP++  
Sbjct: 181 GSSMLAATVAWDNELSWTLENAIEALQETMLQVSSVIMRLRRESLGVIVVSKNHVPSEGA 240

Query: 297 WDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEGESSYQFCVQPA 356
           +  A+N AL+ I    S L+KVVLARS+RI+T  ++DP+ W+A LQ EG+ +YQFC+QP 
Sbjct: 241 YYPAVNNALEIIKDKHSPLSKVVLARSSRIITDTDIDPIAWLARLQCEGQDAYQFCLQPP 300

Query: 357 NAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSNPKDDLEFSIVR 416
            APAFIGNTPERLFHR+ L +  EALAATR RG S + +++IE+DLL++PKDDLEFSIVR
Sbjct: 301 GAPAFIGNTPERLFHRKHLGVCSEALAATRPRGDSKVREMEIERDLLTSPKDDLEFSIVR 360

Query: 417 EAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEILSTLHPTPAVCG 476
           E I+ KL+ +CD VVV+PHKSVRKL R+QHLY+QLAG+L+ +DDEF IL+ LHPTPAVCG
Sbjct: 361 ENIREKLKTICDRVVVKPHKSVRKLARVQHLYSQLAGQLKREDDEFNILTALHPTPAVCG 420

Query: 477 FPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHGALVYAGTGIVK 536
            P EEAR LI + E FDRGMYAGP+G+FGG ESEF+VGIRSALVE G GAL+YAGTGIV 
Sbjct: 421 CPVEEARLLIKQIESFDRGMYAGPIGFFGGGESEFSVGIRSALVEKGLGALIYAGTGIVS 480

Query: 537 GSNPSLEWDELELKISQVTRSLKLELA 561
           GSNPS EW+ELELKISQ T+SL+ E A
Sbjct: 481 GSNPSSEWNELELKISQFTKSLEHESA 507

BLAST of CmaCh11G001620 vs. TAIR 10
Match: AT1G74710.1 (ADC synthase superfamily protein )

HSP 1 Score: 586.6 bits (1511), Expect = 2.0e-167
Identity = 300/516 (58.14%), Postives = 378/516 (73.26%), Query Frame = 0

Query: 54  SPAMDGCRADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLP 113
           S +M+GC  D    + T+ETRT  AV +   ATE L   V++LKS+ P+FSSG++RLQ+P
Sbjct: 45  SMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVP 104

Query: 114 IQQHIKAIHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSS-----------NAHHRLASV 173
           I Q I AI WL +Q  + PR FFS RS     D L  +++           ++   L SV
Sbjct: 105 IDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSV 164

Query: 174 AGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYF 233
           AG+GSAV F     FS+ DW SI+RFLS   PLIRA+GG+RFD    I+ EWEPFG+FYF
Sbjct: 165 AGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYF 224

Query: 234 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISI 293
            VPQVEF+EF   S+LAAT+AWD   SWT ENA+E+LQ T+ QVSS  + ++     +S+
Sbjct: 225 SVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSV 284

Query: 294 LSHNHVPTKTFWDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEG 353
           LS NHVPTK  +  A+ +AL+ I+Q  S L KVVLAR++RI+T  ++DP+ W+A LQ+EG
Sbjct: 285 LSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREG 344

Query: 354 ESSYQFCVQPANAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSN 413
             +YQFC+QP  APAFIGNTPERLF R  L +  EALAATR R  S+  D++IE+DLL++
Sbjct: 345 HDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTS 404

Query: 414 PKDDLEFSIVREAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEIL 473
           PKDDLEFSIVRE I+ KL  +CD VVV+P K+VRKL R+QHLY+QLAGRL  +DDE++IL
Sbjct: 405 PKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKIL 464

Query: 474 STLHPTPAVCGFPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHG 533
           + LHPTPAVCG P EEAR LI E E FDRGMYAGP+G+FGGEESEFAVGIRSALVE G G
Sbjct: 465 AALHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLG 524

Query: 534 ALVYAGTGIVKGSNPSLEWDELELKISQVTRSLKLE 559
           AL+YAGTGIV GS+PS EW+EL+LKISQ T+S++ E
Sbjct: 525 ALIYAGTGIVAGSDPSSEWNELDLKISQFTKSIEYE 560

BLAST of CmaCh11G001620 vs. TAIR 10
Match: AT1G74710.2 (ADC synthase superfamily protein )

HSP 1 Score: 582.0 bits (1499), Expect = 5.0e-166
Identity = 298/509 (58.55%), Postives = 373/509 (73.28%), Query Frame = 0

Query: 54  SPAMDGCRADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGIIRLQLP 113
           S +M+GC  D    + T+ETRT  AV +   ATE L   V++LKS+ P+FSSG++RLQ+P
Sbjct: 45  SMSMNGCDGDFKTPLGTVETRTMTAVLSPAAATERLISAVSELKSQPPSFSSGVVRLQVP 104

Query: 114 IQQHIKAIHWLCSQPLLLPRIFFSGRSRAIDSDPLPSVSS-----------NAHHRLASV 173
           I Q I AI WL +Q  + PR FFS RS     D L  +++           ++   L SV
Sbjct: 105 IDQQIGAIDWLQAQNEIQPRCFFSRRSDVGRPDLLLDLANENGNGNGNGTVSSDRNLVSV 164

Query: 174 AGVGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAFGGIRFDSRANISPEWEPFGSFYF 233
           AG+GSAV F     FS+ DW SI+RFLS   PLIRA+GG+RFD    I+ EWEPFG+FYF
Sbjct: 165 AGIGSAVFFRDLDPFSHDDWRSIRRFLSSTSPLIRAYGGMRFDPNGKIAVEWEPFGAFYF 224

Query: 234 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSSDNVNVQRDLPQISI 293
            VPQVEF+EF   S+LAAT+AWD   SWT ENA+E+LQ T+ QVSS  + ++     +S+
Sbjct: 225 SVPQVEFNEFGGSSMLAATIAWDDELSWTLENAIEALQETMLQVSSVVMKLRNRSLGVSV 284

Query: 294 LSHNHVPTKTFWDRAINRALQAIDQCDSDLTKVVLARSTRIVTSVNVDPVTWVACLQKEG 353
           LS NHVPTK  +  A+ +AL+ I+Q  S L KVVLAR++RI+T  ++DP+ W+A LQ+EG
Sbjct: 285 LSKNHVPTKGAYFPAVEKALEMINQKSSPLNKVVLARNSRIITDTDIDPIAWLAQLQREG 344

Query: 354 ESSYQFCVQPANAPAFIGNTPERLFHREGLSIICEALAATRARGKSAIEDLKIEQDLLSN 413
             +YQFC+QP  APAFIGNTPERLF R  L +  EALAATR R  S+  D++IE+DLL++
Sbjct: 345 HDAYQFCLQPPGAPAFIGNTPERLFQRTQLGVCSEALAATRPRAASSARDMEIERDLLTS 404

Query: 414 PKDDLEFSIVREAIKRKLEAVCDAVVVEPHKSVRKLPRIQHLYAQLAGRLRTKDDEFEIL 473
           PKDDLEFSIVRE I+ KL  +CD VVV+P K+VRKL R+QHLY+QLAGRL  +DDE++IL
Sbjct: 405 PKDDLEFSIVRENIREKLNGICDRVVVKPQKTVRKLARVQHLYSQLAGRLTKEDDEYKIL 464

Query: 474 STLHPTPAVCGFPTEEARDLIAETELFDRGMYAGPVGWFGGEESEFAVGIRSALVETGHG 533
           + LHPTPAVCG P EEAR LI E E FDRGMYAGP+G+FGGEESEFAVGIRSALVE G G
Sbjct: 465 AALHPTPAVCGLPAEEARLLIKEIESFDRGMYAGPIGFFGGEESEFAVGIRSALVEKGLG 524

Query: 534 ALVYAGTGIVKGSNPSLEWDELELKISQV 552
           AL+YAGTGIV GS+PS EW+EL+LKISQV
Sbjct: 525 ALIYAGTGIVAGSDPSSEWNELDLKISQV 553

BLAST of CmaCh11G001620 vs. TAIR 10
Match: AT1G68890.1 (magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-succinyl-5-enolpyruvyl-6-hydroxy-3-cyclohexene-1-carboxylic-acid synthases )

HSP 1 Score: 117.5 bits (293), Expect = 3.5e-26
Identity = 77/226 (34.07%), Postives = 113/226 (50.00%), Query Frame = 0

Query: 49  GVVCQSPAMD-GCRADHGGAVETMETRTFPAVPTVGLATENLRGLVAKLKSEKPAFSSGI 108
           GV    P MD     D    V+   TRT P   T+ L  E+L+  + +LK+  P  SSG+
Sbjct: 56  GVQFDGPIMDRDVNLDDDLVVQVCVTRTLPPALTLELGLESLKEAIDELKTNPPKSSSGV 115

Query: 109 IRLQLPIQQHIKAIHWLCSQPL---LLPRIFFSGRSRAIDSDPLPSVSSNAHHRLASVAG 168
           +R Q+ +    KA+ W CSQP    + P  F S           PS  S        V G
Sbjct: 116 LRFQVAVPPRAKALFWFCSQPTTSDVFPVFFLS------KDTVEPSYKSLYVKEPHGVFG 175

Query: 169 VGSAVVFCRHHQFSNSDWASIKRFLSKKCPLIRAFG--GIRFDSRANISPEWEPFGSFYF 228
           +G+A  F       ++  + IK FLS +  ++ A+G   I F+  + ++ +    GS YF
Sbjct: 176 IGNAFAFVHSSSVDSNGHSMIKTFLSDESAMVTAYGFPDIEFNKYSTVNSK---DGSSYF 235

Query: 229 MVPQVEFDEFEDDSILAATVAWDHACSWTWENAVESLQSTIEQVSS 269
            VPQ+E DE E+ SILA T+AW+ + S+T E  + S + +I QVSS
Sbjct: 236 FVPQIELDEHEEVSILAVTLAWNESLSYTVEQTISSYEKSIFQVSS 272

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9M9V67.1e-17361.02Isochorismate synthase 2, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS2 ... [more]
Q9S7H82.9e-16658.14Isochorismate synthase 1, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=ICS1 ... [more]
Q9ZPC01.7e-16355.92Isochorismate synthase, chloroplastic OS=Catharanthus roseus OX=4058 PE=1 SV=1[more]
P239732.5e-3730.58Isochorismate synthase MenF OS=Bacillus subtilis (strain 168) OX=224308 GN=menF ... [more]
Q515082.6e-3436.12Salicylate biosynthesis isochorismate synthase OS=Pseudomonas aeruginosa (strain... [more]
Match NameE-valueIdentityDescription
AT1G18870.15.0e-17461.02isochorismate synthase 2 [more]
AT1G18870.26.6e-17461.14isochorismate synthase 2 [more]
AT1G74710.12.0e-16758.14ADC synthase superfamily protein [more]
AT1G74710.25.0e-16658.55ADC synthase superfamily protein [more]
AT1G68890.13.5e-2634.07magnesium ion binding;thiamin pyrophosphate binding;hydro-lyases;catalytics;2-su... [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 256..276
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..21
NoneNo IPR availableMOBIDB_LITEmobidb-litedisorder_predictioncoord: 1..26
NoneNo IPR availablePANTHERPTHR47253:SF4ISOCHORISMATE SYNTHASE 2, CHLOROPLASTICcoord: 53..558
IPR015890Chorismate-utilising enzyme, C-terminalPFAMPF00425Chorismate_bindcoord: 294..547
e-value: 5.6E-61
score: 206.3
IPR005801ADC synthaseGENE3D3.60.120.10Anthranilate synthasecoord: 86..557
e-value: 2.5E-105
score: 355.0
IPR005801ADC synthaseSUPERFAMILY56322ADC synthasecoord: 153..556
IPR004561Isochorismate synthaseTIGRFAMTIGR00543TIGR00543coord: 197..555
e-value: 9.2E-107
score: 355.2
IPR044250Isochorismate synthase MenF-likePANTHERPTHR47253FAMILY NOT NAMEDcoord: 53..558

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G001620.1CmaCh11G001620.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0042372 phylloquinone biosynthetic process
biological_process GO:0009058 biosynthetic process
cellular_component GO:0009536 plastid
molecular_function GO:0008909 isochorismate synthase activity