CmaCh11G001360 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh11G001360
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
DescriptionProtein of unknown function (DUF962)
LocationCma_Chr11: 741082 .. 742328 (-)
RNA-Seq ExpressionCmaCh11G001360
SyntenyCmaCh11G001360
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: polypeptideexonCDSthree_prime_UTR
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGGAAGACTGCATTTTTTGATCTGGAAAGGCACTACGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATCCATGTCCTGTTTGTTTGGCCGATTTTCTTCACCACTCTCATGTATTTGTATTTCACCCCTTCTTTCTATACGATTCCCAAATCCCCTTGTGGGTTTGATCATGGCCTGGTTTTGAACTTCGGATTTCTTTTCACCTTAACCTATGCTGCATCTTATGTGATTTTCGATAAGAAAGCAGGGTCCATGGCCGCTTTGCTTTGTTTCGTCTGTTGGGTTGGAGCAAGCTTCATCTCCAATAGACTTGGTTATTCCCAGACTTGGAAGGTAAATTCCCACTTTCTCTTCTTCAATTTAACTCCAGGTTCTTGTAGTAAATTTGTTTGTTTACTCTTATTGAGTGCACTGAAGTTTCTTCCTTGAAATTTCCTTGATGGGTTTTTACTTGCCTCTGCTTACAATCACTATAAGCTTGTAATCTGATCTAATCTGGTTGGATTTGGAAGATGTGTTAAAAGTTTTGACATCTAAGTGGCAGCAAGTTGGGGCCATGTGAAACGGCACTTGGGGTTGATATTGCTGCGCATGGAAGGAGCTTTTATTGCCTGAGCAGTTGGTTGATAATCATAATAGTTCTTTATTTATGACAGATCCTTAGCCTCACCAAAATTTCAAGGCCATTTAGTCCTTGCCAGTTAGAAAACAATTTCAGGACGTGAGGAATGCTCCATACTTCATACGCTAACCTAATTCATTTTTTGTTGTATATCAGGTTGTACTGGCTGCTCAGCTGTTCTGTTGGACAAACCAGATTATAGGCCATGGAGTGTTTGAGGTTAGACAGATCACTCATCTTGACAAATTGATTGCGTCTGTTCTTGAATCTAATTTAATTTGCATTTTTCAATAGAAACGTGCACCGGCTTTGTTGGACAATCTTGCACAAGCCTTTCTAATGGCTCCATTCTTTGTGTTTTTGGAGGTATTCTTTGTCTGGTTTTCTCATCTTCTGAGGGTTGTTTATTGGTGTGGATAATGAAGTAAAGTAGCTTCGTTGTTCCTGTTGGTTGATTTGCAGGTTCTTCAAACTTTATTCAAATATGAACCATATCCAGGGTTTAGTTCAAGTGTGCAAGCAAAGATAGAAGCAGATATAAAAGAGTGGAAAGAAATGAAGGAAAAGCTCTCTTAGGTTGAATCGTCTGGACTTCAATATAAAAAAAAAA

mRNA sequence

ATGGGGAAGACTGCATTTTTTGATCTGGAAAGGCACTACGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATCCATGTCCTGTTTGTTTGGCCGATTTTCTTCACCACTCTCATGTATTTGTATTTCACCCCTTCTTTCTATACGATTCCCAAATCCCCTTGTGGGTTTGATCATGGCCTGGTTTTGAACTTCGGATTTCTTTTCACCTTAACCTATGCTGCATCTTATGTGATTTTCGATAAGAAAGCAGGGTCCATGGCCGCTTTGCTTTGTTTCGTCTGTTGGGTTGGAGCAAGCTTCATCTCCAATAGACTTGGTTATTCCCAGACTTGGAAGGTTGTACTGGCTGCTCAGCTGTTCTGTTGGACAAACCAGATTATAGGCCATGGAGTGTTTGAGAAACGTGCACCGGCTTTGTTGGACAATCTTGCACAAGCCTTTCTAATGGCTCCATTCTTTGTGTTTTTGGAGGTTCTTCAAACTTTATTCAAATATGAACCATATCCAGGGTTTAGTTCAAGTGTGCAAGCAAAGATAGAAGCAGATATAAAAGAGTGGAAAGAAATGAAGGAAAAGCTCTCTTAGGTTGAATCGTCTGGACTTCAATATAAAAAAAAAA

Coding sequence (CDS)

ATGGGGAAGACTGCATTTTTTGATCTGGAAAGGCACTACGCCTTCTATGGCGCTTATCACAGCAACCCAGTCAACATTTTCATCCATGTCCTGTTTGTTTGGCCGATTTTCTTCACCACTCTCATGTATTTGTATTTCACCCCTTCTTTCTATACGATTCCCAAATCCCCTTGTGGGTTTGATCATGGCCTGGTTTTGAACTTCGGATTTCTTTTCACCTTAACCTATGCTGCATCTTATGTGATTTTCGATAAGAAAGCAGGGTCCATGGCCGCTTTGCTTTGTTTCGTCTGTTGGGTTGGAGCAAGCTTCATCTCCAATAGACTTGGTTATTCCCAGACTTGGAAGGTTGTACTGGCTGCTCAGCTGTTCTGTTGGACAAACCAGATTATAGGCCATGGAGTGTTTGAGAAACGTGCACCGGCTTTGTTGGACAATCTTGCACAAGCCTTTCTAATGGCTCCATTCTTTGTGTTTTTGGAGGTTCTTCAAACTTTATTCAAATATGAACCATATCCAGGGTTTAGTTCAAGTGTGCAAGCAAAGATAGAAGCAGATATAAAAGAGTGGAAAGAAATGAAGGAAAAGCTCTCTTAG

Protein sequence

MGKTAFFDLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGFDHGLVLNFGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLAAQLFCWTNQIIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSSSVQAKIEADIKEWKEMKEKLS
Homology
BLAST of CmaCh11G001360 vs. ExPASy Swiss-Prot
Match: O13737 (2-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 972 / ATCC 24843) OX=284812 GN=mpo1 PE=3 SV=2)

HSP 1 Score: 96.7 bits (239), Expect = 3.2e-19
Identity = 68/181 (37.57%), Postives = 90/181 (49.72%), Query Frame = 0

Query: 9   LERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGFDHGLVLNF 68
           L R Y+FY AYHSNPVNI IH + +  +  T L+ L+      T+  S       L +N 
Sbjct: 4   LSRSYSFYAAYHSNPVNIKIHQVCIPLLLLTALVLLH--NFVITLINSK------LQINV 63

Query: 69  GFLFTLTYAASYVIFDKKAGSM-AALLCFVCWVGASFISNRLGYSQTWKVVLAAQLFCWT 128
             L  L Y   YV  D   G + + +L    ++  S +      S   +      + CW 
Sbjct: 64  AHLVGLAYQIFYVTLDPLDGLLYSPVLYLFSYILPSKLFTIFSRSLVNRSAAVVHVICWI 123

Query: 129 NQIIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSSSVQAKIEADI 188
            Q IGHGVFEKR PALLDNL Q+  +AP F FLE         P+ G+  SV +KI A+I
Sbjct: 124 LQFIGHGVFEKRKPALLDNLIQSLFIAPLFAFLET-------GPFVGYYPSVVSKIRANI 169

BLAST of CmaCh11G001360 vs. ExPASy Swiss-Prot
Match: P25338 (2-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATCC 204508 / S288c) OX=559292 GN=MPO1 PE=1 SV=2)

HSP 1 Score: 81.3 bits (199), Expect = 1.4e-14
Identity = 59/181 (32.60%), Postives = 85/181 (46.96%), Query Frame = 0

Query: 8   DLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCGFDHGLVLN 67
           DL     FY  YH NP N+ IH +FV  I F+    L+    + +I          L   
Sbjct: 7   DLRSQLGFYKFYHHNPKNVLIHSIFVPTILFSGSCMLHRVKIYQSI---------SLTAV 66

Query: 68  FGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVLAAQLFCWT 127
              LF++ Y   Y+     AG +  LL        + I +R+    T+K  L      W 
Sbjct: 67  LSVLFSIFYCLLYLPTGLLAGVLLLLL------NLALIDHRV--DLTFKQELGLFTIGWI 126

Query: 128 NQIIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSSSVQAKIEADI 187
            Q +GHGVFEKR PAL+DNL Q+ ++AP+F+  E L  L       GF   ++A +E D+
Sbjct: 127 FQFVGHGVFEKRRPALIDNLVQSLVLAPYFIMFEFLFKL-------GFMPRLKATLEHDL 163

Query: 188 K 189
           +
Sbjct: 187 E 163

BLAST of CmaCh11G001360 vs. TAIR 10
Match: AT1G74440.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 243.4 bits (620), Expect = 1.5e-64
Identity = 119/200 (59.50%), Postives = 148/200 (74.00%), Query Frame = 0

Query: 3   KTAFFDLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLYFTPSFYTIPKSPCG--- 62
           +    DLE+H+AFYGAYHSNP+NI IH LFVWP  F TL++LY TP    +  S  G   
Sbjct: 4   RIGLLDLEKHFAFYGAYHSNPINIIIHTLFVWPNVFATLLFLYSTPPI--LDHSQLGFLK 63

Query: 63  ---FDHGLVLNFGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWK 122
              FD  L L+ GF  T+TYA  Y+  DKK+G +AALLCF CW+G+SF++ RLG+S T K
Sbjct: 64  SLTFDGVLRLDIGFTLTVTYAVFYICLDKKSGVLAALLCFSCWIGSSFLAARLGHSLTLK 123

Query: 123 VVLAAQLFCWTNQIIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFS 182
           V +A+QL CWT Q +GHG+FEKRAPALLDNL QAFLM PFFV LEVLQ++F YEPYPGF 
Sbjct: 124 VGVASQLLCWTGQFLGHGLFEKRAPALLDNLLQAFLMGPFFVLLEVLQSVFGYEPYPGFK 183

Query: 183 SSVQAKIEADIKEWKEMKEK 197
           + V +KIE+ IKEW+E K++
Sbjct: 184 ARVDSKIESGIKEWREKKKQ 201

BLAST of CmaCh11G001360 vs. TAIR 10
Match: AT1G18720.1 (Protein of unknown function (DUF962) )

HSP 1 Score: 242.7 bits (618), Expect = 2.6e-64
Identity = 122/197 (61.93%), Postives = 148/197 (75.13%), Query Frame = 0

Query: 7   FDLERHYAFYGAYHSNPVNIFIHVLFVWPIFFTTLMYLY-FTPSFYTIPKSPCGFDHGLV 66
           FDLE+H+AFYGAYHSNP+NI IH++FVWPIFF+ L+ L+  TP F     S  GF   L 
Sbjct: 8   FDLEKHFAFYGAYHSNPINILIHIIFVWPIFFSVLLLLHSSTPIF---DPSQLGFSQSLT 67

Query: 67  L------NFGFLFTLTYAASYVIFDKKAGSMAALLCFVCWVGASFISNRLGYSQTWKVVL 126
           L      N GF+F L YA  Y+  DKK+G +AAL+CF CWVG+SF++ RLG S   KV L
Sbjct: 68  LDGVLRFNVGFIFALIYALFYIGLDKKSGFVAALMCFSCWVGSSFLAVRLGSSLALKVGL 127

Query: 127 AAQLFCWTNQIIGHGVFEKRAPALLDNLAQAFLMAPFFVFLEVLQTLFKYEPYPGFSSSV 186
           A+QL CWT Q +GHGVFEKRAPALLDNL QAFLMAPFFV LEVLQ++F YEPYPGF + V
Sbjct: 128 ASQLLCWTGQFVGHGVFEKRAPALLDNLLQAFLMAPFFVLLEVLQSVFGYEPYPGFQARV 187

Query: 187 QAKIEADIKEWKEMKEK 197
            AK+E+DIKE++  K+K
Sbjct: 188 NAKVESDIKEYRAKKQK 201

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
O137373.2e-1937.572-hydroxy-palmitic acid dioxygenase mpo1 OS=Schizosaccharomyces pombe (strain 97... [more]
P253381.4e-1432.602-hydroxy-palmitic acid dioxygenase MPO1 OS=Saccharomyces cerevisiae (strain ATC... [more]
Match NameE-valueIdentityDescription
AT1G74440.11.5e-6459.50Protein of unknown function (DUF962) [more]
AT1G18720.12.6e-6461.93Protein of unknown function (DUF962) [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 180..198
NoneNo IPR availablePANTHERPTHR28026:SF8YGL010W-LIKE PROTEINcoord: 1..197
IPR0093052-hydroxy-palmitic acid dioxygenase Mpo1-likePFAMPF06127DUF962coord: 7..165
e-value: 1.5E-29
score: 102.0
IPR0093052-hydroxy-palmitic acid dioxygenase Mpo1-likePANTHERPTHR28026DUF962 DOMAIN PROTEIN (AFU_ORTHOLOGUE AFUA_8G05310)coord: 1..197

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh11G001360.1CmaCh11G001360.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0046521 sphingoid catabolic process
cellular_component GO:0005783 endoplasmic reticulum
cellular_component GO:0016021 integral component of membrane