CmaCh10G011330 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh10G011330
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	Pectinesterase
Location	Cma_Chr10: 7823071 .. 7825637 (+)
RNA-Seq Expression	CmaCh10G011330
Synteny	CmaCh10G011330

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonfive_prime_UTRCDSpolypeptidethree_prime_UTR

Hold the cursor over a type above to highlight its positions in the sequence below.

ATTCAAGGTAAAAAAAAAAAAAAAAGACACAACATGGCTTATGATGAAGATGATTCTGGGAACAATAAGAGATACGCCATCATCGGTGTCTCGTCGATGTTACTAGTCGCAATGGTGGTGGCAGTCACGGTGGGCGTTAACCTAAACAATGACGAAGCGGGCGGTCCGACCTCTGGAACCAAATCCAATGAAATATCTGCTTCGGTGAAAGCCATTAAATCCATTTGCCAAACCACGGATTATAAAAAGGACTGTGTTGATAGTCTTAAGTCTAATGCTAACAACTCTGCTGACCCACATGAATTGGTTAAAGCTGGGTTCAAAGCTGCCATGAAGTTACTCCAAGCCGCTGCTAAACAATCTGTCACCTTGCAACAACTTGAGCAAGACCCTAGAGCCTCCCAAGCCCTTGCTGGCTGTAAGGAGCTCATGGACTTCGCCATCGACGAGCTTAAATTCTCAATGAGTAGGTTTGGTGATTTCGACACTACGCACCTCGATGAAATGTTGATCGATCTTAAGATCTGGCTCAGTGCCACCATTACGTACCAGGTAATCTTACGTGGGCGAGTTTTGTTCGTTTAAAGTTGATGGGTTATGATAAATTTTTGAATTGGTGTGGGAAACTATTACCTGGGCGAGTGTTGTTCGTTTAAAGTTGATGGGTTATGGTAAATTTTTGAATTGGTGTGGGAAACTATTACCTGGGCTGAGTGTTGTTCGTTTAAAGTTACGGGTTATGGTAAATTTTTGAATTGGTATGGGAAACTATTACCTGGGCTGAGTGTTGTTCGTTTAAAGTTGATGGGTTATGATTTATTTTTGAATTGGTGTAGGAAACTTGCTTGGATTCGTTTGAAAACACGACGGGGGATGCGTCGGAGAAGATGAAGAAGGCATTGAAGACGTCGATGCGGTTGAGTAGCAATGGATTGGCGATGGTGACCGAGATCTCATCCATGTTGACAGATTTGAAGGTGCCAGGGATCGGCCGACGGCGACTTCTTGATGCTCCTGTTGTCGTCGGGCATGAAGATTACCCCGAGTGGGCGGACCCCGGGATACGGCGGCTACTGGCGGCGAAGGGGAATGTGAAGGCAAACTTTGTGGTGGCTGTGGATGGAAGTGGGGATTTCAAGAGCATTCAATCAGCTATTGACAAAGTTCCAAAGAGGAAGAACAACGAAACTTTTGTGATTCATATCAAGGAAGGAGTGTATAAGGAATACGTGACCATTAAAAAATCCATCACCCATTTGATGCTCATCGGCGATGGCCCGAAAAAGACGATCATCAGTGGCCATAAGAACTTCTTCGATGGCATTACCACATTCAAAACTGCAACCGTTGGTTAGTATACTAATTTCGTAGATACAATTCAACATTTTGTTCGTTCGGATTTGATTTTGTCATAAAATTAGGTTTATCTTTTCATAACTCTATAGTTTGAATTAGGTTATCTTTGGATGAGCTTGCATTGCTGATATTGACACGAATTAGGATATCTTTGGATGAGCTTGCATTGCTGATGTTGACCCGAGTTAGGATATCTTTGGATGAGCTTGCATTGCTGATGTTGACCGAGTTAGGTTATCTTTGGATGAACTTGCATTGCTGATATTGACCCGAGTTAGGTTATCTTTAGATGAACTTGCATTGCTGATATTGACCCGAGTTAGATTATCTATGGATGAACTTGCATTGCTGATATTGACCCGAGTTAGGTTCAAATAATTGCATGATATTAGTTCAAATAATTGCATGCAGCTGTGACGGCAGAGCACTTCCGGGCAAGAGACATAGGGTTCGTGAACACAGCAGGACCACACAAGCACCAAGCAGTAGCATTACGAGTCCAAGCAGACAAAGCCATATTCTTCAACTGTGAGATGCATGGCTACCAAGACACACTCTACGTCCACACCATGCGACAATTCTACAGAGACTGCATCGTCTCTGGCACCATCGACTTCATCTTCGGCGATGCAGCCGTCGTCTTCCAAAACTGCATCTTCCTAATCCGCAAGCCGCTTTCAAATCAACAAAACATCGTCACAGCACACGGCCGGAAGGAGCGCCGACAACCCTCCGCCCTAATCATCCAAAACTGCACCTTCAAGGCGGACACGGACCTCGCCCCAGTGAAAAATGTGTTCCGATCCTTCCTCGGGAGACCGTGGAAGGAATATTCAAGAACAATCATCATGGAATCATACATCGACGATGTGATTCATCCAGACGGGTGGCTGCCGTGGAGCGGCGACTGGGGGTTGAAGACATGCTTTTACACGGAGTTTCGGAACTACGGGCCAGGGTCGGACACGTCGAAGAGGGTGAAATGGAGGGGGGTGAAGGTTCTTACGCCGGCGCATGCGGTGGATTTCACGGCGAGCAGGTTTTTATTGGGAGATAGATGGATAAAGGCCACCGGAGTACCGTACGCGCCGGGGTTGACAATGAGAAGACACAGTGAGGTGTAGCTTATTGATTTTGGTGAGCAACATTTTTAATTCTTTTGAAATGGCGGCCGGCGACCAGGCTTAAGGCCGTAAGGGCCGTGTGTAGAG

mRNA sequence

ATTCAAGGTAAAAAAAAAAAAAAAAGACACAACATGGCTTATGATGAAGATGATTCTGGGAACAATAAGAGATACGCCATCATCGGTGTCTCGTCGATGTTACTAGTCGCAATGGTGGTGGCAGTCACGGTGGGCGTTAACCTAAACAATGACGAAGCGGGCGGTCCGACCTCTGGAACCAAATCCAATGAAATATCTGCTTCGGTGAAAGCCATTAAATCCATTTGCCAAACCACGGATTATAAAAAGGACTGTGTTGATAGTCTTAAGTCTAATGCTAACAACTCTGCTGACCCACATGAATTGGTTAAAGCTGGGTTCAAAGCTGCCATGAAGTTACTCCAAGCCGCTGCTAAACAATCTGTCACCTTGCAACAACTTGAGCAAGACCCTAGAGCCTCCCAAGCCCTTGCTGGCTGTAAGGAGCTCATGGACTTCGCCATCGACGAGCTTAAATTCTCAATGAGTAGGTTTGGTGATTTCGACACTACGCACCTCGATGAAATGTTGATCGATCTTAAGATCTGGCTCAGTGCCACCATTACGTACCAGGAAACTTGCTTGGATTCGTTTGAAAACACGACGGGGGATGCGTCGGAGAAGATGAAGAAGGCATTGAAGACGTCGATGCGGTTGAGTAGCAATGGATTGGCGATGGTGACCGAGATCTCATCCATGTTGACAGATTTGAAGGTGCCAGGGATCGGCCGACGGCGACTTCTTGATGCTCCTGTTGTCGTCGGGCATGAAGATTACCCCGAGTGGGCGGACCCCGGGATACGGCGGCTACTGGCGGCGAAGGGGAATGTGAAGGCAAACTTTGTGGTGGCTGTGGATGGAAGTGGGGATTTCAAGAGCATTCAATCAGCTATTGACAAAGTTCCAAAGAGGAAGAACAACGAAACTTTTGTGATTCATATCAAGGAAGGAGTGTATAAGGAATACGTGACCATTAAAAAATCCATCACCCATTTGATGCTCATCGGCGATGGCCCGAAAAAGACGATCATCAGTGGCCATAAGAACTTCTTCGATGGCATTACCACATTCAAAACTGCAACCGTTGCTGTGACGGCAGAGCACTTCCGGGCAAGAGACATAGGGTTCGTGAACACAGCAGGACCACACAAGCACCAAGCAGTAGCATTACGAGTCCAAGCAGACAAAGCCATATTCTTCAACTGTGAGATGCATGGCTACCAAGACACACTCTACGTCCACACCATGCGACAATTCTACAGAGACTGCATCGTCTCTGGCACCATCGACTTCATCTTCGGCGATGCAGCCGTCGTCTTCCAAAACTGCATCTTCCTAATCCGCAAGCCGCTTTCAAATCAACAAAACATCGTCACAGCACACGGCCGGAAGGAGCGCCGACAACCCTCCGCCCTAATCATCCAAAACTGCACCTTCAAGGCGGACACGGACCTCGCCCCAGTGAAAAATGTGTTCCGATCCTTCCTCGGGAGACCGTGGAAGGAATATTCAAGAACAATCATCATGGAATCATACATCGACGATGTGATTCATCCAGACGGGTGGCTGCCGTGGAGCGGCGACTGGGGGTTGAAGACATGCTTTTACACGGAGTTTCGGAACTACGGGCCAGGGTCGGACACGTCGAAGAGGGTGAAATGGAGGGGGGTGAAGGTTCTTACGCCGGCGCATGCGGTGGATTTCACGGCGAGCAGGTTTTTATTGGGAGATAGATGGATAAAGGCCACCGGAGTACCGTACGCGCCGGGGTTGACAATGAGAAGACACAGTGAGGTGTAGCTTATTGATTTTGGTGAGCAACATTTTTAATTCTTTTGAAATGGCGGCCGGCGACCAGGCTTAAGGCCGTAAGGGCCGTGTGTAGAG

Coding sequence (CDS)

ATGGCTTATGATGAAGATGATTCTGGGAACAATAAGAGATACGCCATCATCGGTGTCTCGTCGATGTTACTAGTCGCAATGGTGGTGGCAGTCACGGTGGGCGTTAACCTAAACAATGACGAAGCGGGCGGTCCGACCTCTGGAACCAAATCCAATGAAATATCTGCTTCGGTGAAAGCCATTAAATCCATTTGCCAAACCACGGATTATAAAAAGGACTGTGTTGATAGTCTTAAGTCTAATGCTAACAACTCTGCTGACCCACATGAATTGGTTAAAGCTGGGTTCAAAGCTGCCATGAAGTTACTCCAAGCCGCTGCTAAACAATCTGTCACCTTGCAACAACTTGAGCAAGACCCTAGAGCCTCCCAAGCCCTTGCTGGCTGTAAGGAGCTCATGGACTTCGCCATCGACGAGCTTAAATTCTCAATGAGTAGGTTTGGTGATTTCGACACTACGCACCTCGATGAAATGTTGATCGATCTTAAGATCTGGCTCAGTGCCACCATTACGTACCAGGAAACTTGCTTGGATTCGTTTGAAAACACGACGGGGGATGCGTCGGAGAAGATGAAGAAGGCATTGAAGACGTCGATGCGGTTGAGTAGCAATGGATTGGCGATGGTGACCGAGATCTCATCCATGTTGACAGATTTGAAGGTGCCAGGGATCGGCCGACGGCGACTTCTTGATGCTCCTGTTGTCGTCGGGCATGAAGATTACCCCGAGTGGGCGGACCCCGGGATACGGCGGCTACTGGCGGCGAAGGGGAATGTGAAGGCAAACTTTGTGGTGGCTGTGGATGGAAGTGGGGATTTCAAGAGCATTCAATCAGCTATTGACAAAGTTCCAAAGAGGAAGAACAACGAAACTTTTGTGATTCATATCAAGGAAGGAGTGTATAAGGAATACGTGACCATTAAAAAATCCATCACCCATTTGATGCTCATCGGCGATGGCCCGAAAAAGACGATCATCAGTGGCCATAAGAACTTCTTCGATGGCATTACCACATTCAAAACTGCAACCGTTGCTGTGACGGCAGAGCACTTCCGGGCAAGAGACATAGGGTTCGTGAACACAGCAGGACCACACAAGCACCAAGCAGTAGCATTACGAGTCCAAGCAGACAAAGCCATATTCTTCAACTGTGAGATGCATGGCTACCAAGACACACTCTACGTCCACACCATGCGACAATTCTACAGAGACTGCATCGTCTCTGGCACCATCGACTTCATCTTCGGCGATGCAGCCGTCGTCTTCCAAAACTGCATCTTCCTAATCCGCAAGCCGCTTTCAAATCAACAAAACATCGTCACAGCACACGGCCGGAAGGAGCGCCGACAACCCTCCGCCCTAATCATCCAAAACTGCACCTTCAAGGCGGACACGGACCTCGCCCCAGTGAAAAATGTGTTCCGATCCTTCCTCGGGAGACCGTGGAAGGAATATTCAAGAACAATCATCATGGAATCATACATCGACGATGTGATTCATCCAGACGGGTGGCTGCCGTGGAGCGGCGACTGGGGGTTGAAGACATGCTTTTACACGGAGTTTCGGAACTACGGGCCAGGGTCGGACACGTCGAAGAGGGTGAAATGGAGGGGGGTGAAGGTTCTTACGCCGGCGCATGCGGTGGATTTCACGGCGAGCAGGTTTTTATTGGGAGATAGATGGATAAAGGCCACCGGAGTACCGTACGCGCCGGGGTTGACAATGAGAAGACACAGTGAGGTGTAG

Protein sequence

MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKAIKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDPRASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSFENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHEDYPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGVYKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVNTAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAVVFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGRPWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVKVLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV

Homology

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BLAST of CmaCh10G011330 vs. ExPASy Swiss-Prot
Match: Q3E8Z8 (Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3702 GN=PME28 PE=2 SV=1)

HSP 1 Score: 628.6 bits (1620), Expect = 6.9e-179
Identity = 304/572 (53.15%), Postives = 411/572 (71.85%), Query Frame = 0


Query: 3   YDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKAIK 62
           YD++D+   KRY II +SS+LL++MVVAVT+GV++N  +  G        EI+ SVKAIK
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSVKAIK 64

Query: 63  SICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDPRA 122
            +C  TDYK+ C D+L+ +A +++DP ELVK  F A MK +   AK+S T+ +L++DPRA
Sbjct: 65  DVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRA 124

Query: 123 SQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSFEN 182
             AL  CKELMD+AI EL  S    G F+   +DE L+ L+IWLSATI++++TCLD F+ 
Sbjct: 125 KMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQG 184

Query: 183 TTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHEDYP 242
           T G+A E +KKALKT+++L+ NGLAMVTE+S+ L  +++P +  RRLL        +++P
Sbjct: 185 TQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS-------QEFP 244

Query: 243 EWADPGIRRLL-AAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGVY 302
            W D   RRLL A    VK + VVA DGSG +K+I  A++ VPK+KN  TFV+HIKEG+Y
Sbjct: 245 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNT-TFVVHIKEGIY 304

Query: 303 KEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVNT 362
           KEYV + +S+THL+ IGDGP KT+ISG K++ DGITT+KTATVA+  +HF A++I F NT
Sbjct: 305 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 364

Query: 363 AGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAVV 422
           AG  KHQAVA+RV AD++IF+NC+  GYQDTLY H+ RQFYRDC +SGTIDF+FGDAA V
Sbjct: 365 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 424

Query: 423 FQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGRP 482
           FQNC  L+RKPL NQ   +TAHGRK+ R+ +  ++Q CT   + D   VK   +++LGRP
Sbjct: 425 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 484

Query: 483 WKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVKV 542
           WKEYSRTIIM ++I D + P+GW PW G++GL T FY+E +N GPG+  +KRV W G+K 
Sbjct: 485 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 544

Query: 543 LTPAHAVDFTASRFLLGDRWIKATGVPYAPGL 574
           L+    + FT ++++ GD WI   GVPY  GL
Sbjct: 545 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

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BLAST of CmaCh10G011330 vs. ExPASy Swiss-Prot
Match: Q8GX86 (Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3702 GN=PME21 PE=2 SV=2)

HSP 1 Score: 620.2 bits (1598), Expect = 2.4e-176
Identity = 306/577 (53.03%), Postives = 406/577 (70.36%), Query Frame = 0


Query: 1   MAYDEDDSGNNK-RYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVK 60
           M+Y  DD    K RY +I +SS+LL++MVVAVTVGV+LN  +  G + G    E++ASVK
Sbjct: 1   MSYGYDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHD--GDSKG--KAEVNASVK 60

Query: 61  AIKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQD 120
           A+K +C  TDY+K C D+L  N  N+ DP ELVK  F   MK +  AAK+S T+ +L++D
Sbjct: 61  AVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKD 120

Query: 121 PRASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDS 180
            R   AL  CKELMD+A+DEL  S    G F+   LDE LI+L+IWLSA I+++ETCL+ 
Sbjct: 121 SRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEG 180

Query: 181 FENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHE 240
           F+ T G+A E MKKALKT++ L+ NGLA+++E+S+ +  +++PG+  RRLL        E
Sbjct: 181 FQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLL-------AE 240

Query: 241 DYPEWADPGIRRLL---AAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHI 300
            +P W D   R+LL   AA  +VK + VVA DGSG +K+I  A+  VPK K N TFV+HI
Sbjct: 241 GFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHI 300

Query: 301 KEGVYKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDI 360
           K G+YKEYV + K+++HL+ IGDGP KTIISG+KN+ DGITT++TATVA+   +F A++I
Sbjct: 301 KAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNI 360

Query: 361 GFVNTAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFG 420
           GF NTAG  KHQAVA+RVQ+D++IFFNC   GYQDTLY H+ RQF+RDC +SGTIDF+FG
Sbjct: 361 GFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFG 420

Query: 421 DAAVVFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRS 480
           DAA VFQNC  L+RKPL NQ   +TAHGRK+ R+ +  + Q CT   + D   VK   ++
Sbjct: 421 DAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKA 480

Query: 481 FLGRPWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKW 540
           +LGRPWKEYSRTIIM ++I D + P GW PW GD+GLKT FY+E +N GPGS  + RV W
Sbjct: 481 YLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTW 540

Query: 541 RGVKVLTPAHAVDFTASRFLLGDRWIKATGVPYAPGL 574
            G+K L+    + FT ++++ GD WI   GVPY  GL
Sbjct: 541 AGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

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BLAST of CmaCh10G011330 vs. ExPASy Swiss-Prot
Match: Q9FJ21 (Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3702 GN=PME58 PE=2 SV=1)

HSP 1 Score: 525.8 bits (1353), Expect = 6.3e-148
Identity = 269/575 (46.78%), Postives = 377/575 (65.57%), Query Frame = 0


Query: 6   DDSGNNKRYAIIGVSSMLLVAMVVAV--TVGVNLNNDEAGGPTSGTKSNEISASVKAIKS 65
           D     K+  I GV + LLV MVVAV  T   N ++ E   P       +I  +  A+++
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQ-SVTL-QQLEQDPR 125
           +C  TDYK+ CV+SL   + +S  P +L+K GF   ++ ++ + K+ SV L  +   D  
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 ASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSFE 185
              AL  C++LM+ A D+LK  +  F  F    +++ + DL++WLS +I YQ+TC+D+FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIG------RRRLLDAPVV 245
            T    S+ M+K  KTS  L+SNGLAM+T IS++L +  V G+        R+LL A   
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA--- 243

Query: 246 VGHEDYPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIH 305
              +  P W  P  RRL+A KG VKAN VVA DGSG +K+I  A++ VPK  N + FVI+
Sbjct: 244 --EDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIY 303

Query: 306 IKEGVYKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDG-ITTFKTATVAVTAEHFRAR 365
           IK+GVY E V + K +TH+  IGDGP KT I+G  N++ G + T+ TATVA+  ++F A+
Sbjct: 304 IKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAK 363

Query: 366 DIGFVNTAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFI 425
           +IGF NTAGP  HQAVALRV AD A+F+NC++ GYQDTLYVH+ RQF+RDC VSGT+DFI
Sbjct: 364 NIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFI 423

Query: 426 FGDAAVVFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVF 485
           FGD  VV QNC  ++RKP+ +Q  ++TA GR ++R+ + L++QNC    +    PVK++ 
Sbjct: 424 FGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSIN 483

Query: 486 RSFLGRPWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRV 545
           +++LGRPWKE+SRTIIM + IDDVI P GWLPW+GD+ L T +Y E+ N GPGS+ ++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRV 543

Query: 546 KWRGVKVLTPAHAVDFTASRFLLGDRWIKATGVPY 570
           KW G+K L+P  A+ FT +RFL G+ WI    VPY
Sbjct: 544 KWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of CmaCh10G011330 vs. ExPASy Swiss-Prot
Match: Q8GXA1 (Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3702 GN=PME23 PE=2 SV=3)

HSP 1 Score: 496.5 bits (1277), Expect = 4.1e-139
Identity = 263/571 (46.06%), Postives = 364/571 (63.75%), Query Frame = 0


Query: 7   DSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKAIKSICQ 66
           D    K++ + G  S  LV MVV+V V  + ++ +          N I  + KA++++C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPK-------DDENHIRKTTKAVQAVCA 63

Query: 67  TTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLL-QAAAKQSVTLQ-QLEQDPRASQ 126
            TD+K  CV+SL   + +S DP +L+K GFK  +K + ++  K S  ++ + +++P A  
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSFENTT 186
           A   C++LM  AID+LK  M     F    ++  + DL++WLS +I +Q+TC+DSF    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED-YPE 246
            +  + M K  KTS  LSSN LAMVT IS+++ +  + G+       A  ++  ED  P 
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPT 243

Query: 247 WADPGIRRLLAAKGN----VKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEG 306
           W  P  RRL+AA+G     VKAN VVA DG+G FK+I  A++ VPK  N   F+IHIKEG
Sbjct: 244 WVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIHIKEG 303

Query: 307 VYKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDG-ITTFKTATVAVTAEHFRARDIGF 366
           +YKE VT+ K + H+  IGDGP KT+I+G  NF  G + TF TAT+ +  +HF A++IG 
Sbjct: 304 IYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGI 363

Query: 367 VNTAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDA 426
            NTAGP   QAVALRV AD A+F +C++ G+QDTLYVH+ RQFYRDC VSGT+DFIFGDA
Sbjct: 364 ENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDA 423

Query: 427 AVVFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFL 486
             + QNC  ++RKP   Q  +VTA GR   R+ + L++  C    D    P+K+V +++L
Sbjct: 424 KCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYL 483

Query: 487 GRPWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRG 546
           GRPWKE+SRTIIM++ IDDVI P GWLPWSGD+ LKT +Y E  N GPGS+ ++RVKW G
Sbjct: 484 GRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPG 543

Query: 547 VKVLTPAHAVDFTASRFLLGDRWIKATGVPY 570
           +K LTP  A+ +T  RFL GD WI  T VPY
Sbjct: 544 IKKLTPQDALLYTGDRFLRGDTWIPQTQVPY 564

[Close]

BLAST of CmaCh10G011330 vs. ExPASy Swiss-Prot
Match: O23447 (Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3702 GN=PME43 PE=2 SV=1)

HSP 1 Score: 440.7 bits (1132), Expect = 2.7e-122
Identity = 261/702 (37.18%), Postives = 367/702 (52.28%), Query Frame = 0


Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKAIKSICQTTDYKK 72
           +Y ++GV++ L++AMV+ V    N  N  +       + +++  +   +  IC +TDYK+
Sbjct: 3   KYVLLGVTA-LIMAMVICVE--ANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQ 62

Query: 73  DCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSV--TLQQLEQDPRASQALAGCK 132
           DC  SL +    S DP  L+++ F  A+  +++   + +     + + D    +AL  C+
Sbjct: 63  DCTTSLAT--VRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCR 122

Query: 133 ELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSFENTTGDASEK 192
           ELMD AID+L+ +  +F  F  T L + + DL +WLS +ITYQ+TC+D FE    +A+  
Sbjct: 123 ELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVM 182

Query: 193 MKKALKTSMRLSSNGLAMVTEISSMLTDLKVP------GIGRRRLL-------------- 252
           M++ ++    L+SNGLA+   +  +L   ++P        GR  +L              
Sbjct: 183 MERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQ 242

Query: 253 ----------DAPVVV-------------------------------------------- 312
                     D+P  V                                            
Sbjct: 243 DSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSA 302

Query: 313 --GHE------------------------------------------------------- 372
             G++                                                       
Sbjct: 303 FSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLK 362

Query: 373 --------DYPEWADPGIRRLLAAK---GNVKANFVVAVDGSGDFKSIQSAIDKVPKRKN 432
                   ++P W  P  RRLLA +     +KAN VVA DGSG  K+I  A+  VP  KN
Sbjct: 363 DRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-MKN 422

Query: 433 NETFVIHIKEGVYKEYVTIKKSITHLMLIGDGPKKTIISGHKNFF-DGITTFKTATVAVT 492
            + FVIHIKEGVYKE V + K + H+M +GDGP KT+I+G   F  D + T++TA+VAV 
Sbjct: 423 TKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVN 482

Query: 493 AEHFRARDIGFVNTAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIV 552
            ++F A+DIGF NTAG  +HQAVALRV AD A+FFNC M+GYQDTLYVHT RQFYR+C V
Sbjct: 483 GDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRV 542

Query: 553 SGTIDFIFGDAAVVFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDL 570
           SGTIDF+FGDA  VFQNC F+IR+P+ +QQ IVTA GRK+RR+ + ++I N     D   
Sbjct: 543 SGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASY 602

[Close]

BLAST of CmaCh10G011330 vs. ExPASy TrEMBL
Match: A0A6J1HU87 (Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467859 PE=3 SV=1)

HSP 1 Score: 1171.8 bits (3030), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0


Query: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60
           MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA
Sbjct: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60

Query: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120
           IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP
Sbjct: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120

Query: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180
           RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF
Sbjct: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180

Query: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240
           ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED
Sbjct: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240

Query: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300
           YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV
Sbjct: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300

Query: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360
           YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN
Sbjct: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360

Query: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420
           TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV
Sbjct: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420

Query: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480
           VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR
Sbjct: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540
           PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK
Sbjct: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540

Query: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV 582
           VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV
Sbjct: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV 581

[Close]

BLAST of CmaCh10G011330 vs. ExPASy TrEMBL
Match: A0A6J1HEC9 (Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111462093 PE=3 SV=1)

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 559/581 (96.21%), Postives = 570/581 (98.11%), Query Frame = 0


Query: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60
           MAYDEDDSG+NKRYAIIGVSSMLL+AMVVAVTVGVNLNN EAGGPT+GTKSNEISASVKA
Sbjct: 1   MAYDEDDSGSNKRYAIIGVSSMLLIAMVVAVTVGVNLNNGEAGGPTTGTKSNEISASVKA 60

Query: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120
           IKSICQ+TDYKKDCVDSLKSNANNS+DPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP
Sbjct: 61  IKSICQSTDYKKDCVDSLKSNANNSSDPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120

Query: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180
           RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDE+LIDLKIWLSATITYQETCLDSF
Sbjct: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEILIDLKIWLSATITYQETCLDSF 180

Query: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240
           ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGH+D
Sbjct: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHDD 240

Query: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300
           YPEWADPGIRRLLAAKG VKANFVVAVDGSGDF +IQSAIDKVPKRKNNETFVIHIKEGV
Sbjct: 241 YPEWADPGIRRLLAAKGKVKANFVVAVDGSGDFNNIQSAIDKVPKRKNNETFVIHIKEGV 300

Query: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360
           YKEYV+IKKSITHLMLIGDGPKKTIISG KNF DGITTFKTAT A TAEHF ARDIGFVN
Sbjct: 301 YKEYVSIKKSITHLMLIGDGPKKTIISGQKNFVDGITTFKTATFAATAEHFLARDIGFVN 360

Query: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420
           TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHT+RQFYRDCIVSGTIDFIFGDAAV
Sbjct: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTLRQFYRDCIVSGTIDFIFGDAAV 420

Query: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480
           VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKN FRSFLGR
Sbjct: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNEFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540
           PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK
Sbjct: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540

Query: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV 582
           VLTPAHAVDFTASRFLLGDRWIKATG PYAPGLTM+ HSEV
Sbjct: 541 VLTPAHAVDFTASRFLLGDRWIKATGTPYAPGLTMKGHSEV 581

[Close]

BLAST of CmaCh10G011330 vs. ExPASy TrEMBL
Match: A0A5D3CGC3 (Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 1.1e-267
Identity = 445/574 (77.53%), Postives = 512/574 (89.20%), Query Frame = 0


Query: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60
           M Y +DDS N KRYAIIGVSSMLLVAMVVAVTVGVNLN DE   P +G KS+EIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120
           IK+ICQ TDYK++C+ SLK+  NNS+DP ELV+AGFKAAMKL+QAAA +SV L+QLE+DP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180
           R S+ALAGCKELMDFAIDELK+SM++ G+FD T LDEMLID++IWLSATITYQETCLD F
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240
           +NTTG+A+EKMKKALKTSM+LSSNGLAMV++ SSMLT+L++PGI RRRLL+ P V+GH+D
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIP-VLGHDD 240

Query: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300
           YP+WA+PG+RRLLAA   VK N VVA DGSG F++IQ+A+D+VPKRKNN T+VIHIK GV
Sbjct: 241 YPDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGV 300

Query: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360
           Y+EY+ IKK++THLMLIGDGPKKTII+G+KNF DG  TFKTATVAVTAEHF ARDIGF N
Sbjct: 301 YQEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420
           TAGP KHQAVALRVQADKA+F+NCEMHGYQDTLYVHTMRQFYRDC VSGTIDFIFGDAA 
Sbjct: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420

Query: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480
           +FQ C FL+RKPL NQQ IVTAHGRKERRQPSALIIQNC+ KAD DLAPV+  FRSFLGR
Sbjct: 421 IFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540
           PWKEYSRTIIMESYI D+I P+GWLPW+GDWGLKTCFYTE+ NYGPGS+ SKRVKWRG+K
Sbjct: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIK 540

Query: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLT 575
            +TP HA+DFTA RF++GDRWIK TGVPY  G+T
Sbjct: 541 TITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMT 573

[Close]

BLAST of CmaCh10G011330 vs. ExPASy TrEMBL
Match: A0A1S3BPI8 (Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1)

HSP 1 Score: 932.2 bits (2408), Expect = 1.1e-267
Identity = 445/574 (77.53%), Postives = 512/574 (89.20%), Query Frame = 0


Query: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60
           M Y +DDS N KRYAIIGVSSMLLVAMVVAVTVGVNLN DE   P +G KS+EIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120
           IK+ICQ TDYK++C+ SLK+  NNS+DP ELV+AGFKAAMKL+QAAA +SV L+QLE+DP
Sbjct: 61  IKAICQPTDYKQECIASLKATGNNSSDPQELVQAGFKAAMKLIQAAANKSVDLKQLEKDP 120

Query: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180
           R S+ALAGCKELMDFAIDELK+SM++ G+FD T LDEMLID++IWLSATITYQETCLD F
Sbjct: 121 RTSKALAGCKELMDFAIDELKYSMNKLGEFDITKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240
           +NTTG+A+EKMKKALKTSM+LSSNGLAMV++ SSMLT+L++PGI RRRLL+ P V+GH+D
Sbjct: 181 QNTTGNAAEKMKKALKTSMKLSSNGLAMVSQFSSMLTELQIPGISRRRLLEIP-VLGHDD 240

Query: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300
           YP+WA+PG+RRLLAA   VK N VVA DGSG F++IQ+A+D+VPKRKNN T+VIHIK GV
Sbjct: 241 YPDWANPGMRRLLAAGSRVKPNVVVAKDGSGQFRTIQAALDQVPKRKNNATYVIHIKAGV 300

Query: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360
           Y+EY+ IKK++THLMLIGDGPKKTII+G+KNF DG  TFKTATVAVTAEHF ARDIGF N
Sbjct: 301 YQEYLQIKKTVTHLMLIGDGPKKTIITGNKNFVDGTPTFKTATVAVTAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420
           TAGP KHQAVALRVQADKA+F+NCEMHGYQDTLYVHTMRQFYRDC VSGTIDFIFGDAA 
Sbjct: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420

Query: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480
           +FQ C FL+RKPL NQQ IVTAHGRKERRQPSALIIQNC+ KAD DLAPV+  FRSFLGR
Sbjct: 421 IFQGCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSIKADKDLAPVQKQFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540
           PWKEYSRTIIMESYI D+I P+GWLPW+GDWGLKTCFYTE+ NYGPGS+ SKRVKWRG+K
Sbjct: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSNKSKRVKWRGIK 540

Query: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLT 575
            +TP HA+DFTA RF++GDRWIK TGVPY  G+T
Sbjct: 541 TITPQHALDFTAGRFVMGDRWIKPTGVPYVSGMT 573

[Close]

BLAST of CmaCh10G011330 vs. ExPASy TrEMBL
Match: A0A0A0LYF5 (Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1)

HSP 1 Score: 929.5 bits (2401), Expect = 6.9e-267
Identity = 448/574 (78.05%), Postives = 508/574 (88.50%), Query Frame = 0


Query: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60
           M Y +DDS N KRYAIIGVSSMLLVAMVVAVTVGVNLN DE   P +G KS+EIS+S+KA
Sbjct: 1   MGYKDDDSQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQDETSDPATGNKSHEISSSMKA 60

Query: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120
           IK+ICQ TDYK++CV SLK+  NNS+DP ELV+AGFKAAMKL+QAAA +SV L QLE+DP
Sbjct: 61  IKAICQPTDYKQECVASLKATGNNSSDPKELVQAGFKAAMKLIQAAANKSVALNQLEKDP 120

Query: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180
           RAS+ALAGCKELMDFAIDELK+SM++ G+FD + LDEMLID++IWLSATITYQETCLD F
Sbjct: 121 RASKALAGCKELMDFAIDELKYSMNKLGEFDISKLDEMLIDIRIWLSATITYQETCLDGF 180

Query: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240
            NTTG+A+EKMKKALKTSM+LSSNGLAMV++ISSML++L++PGI RRRLL+ P V+GH+D
Sbjct: 181 ANTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLSELQIPGISRRRLLEIP-VLGHDD 240

Query: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300
           YP+WA+PG+RRLLAA   VK N VVA DGSG FK+IQ AID+VPKRKNN T+VIHIK GV
Sbjct: 241 YPDWANPGMRRLLAAGSKVKPNVVVAKDGSGQFKTIQEAIDQVPKRKNNATYVIHIKAGV 300

Query: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360
           Y+EYV +KK++THLMLIGDGPKKTII+G+KNF DG  TFKTATVAVTAEHF ARDIGF N
Sbjct: 301 YQEYVLVKKTLTHLMLIGDGPKKTIITGNKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420
           TAGP KHQAVALRVQADKA+F+NCEMHGYQDTLYVHTMRQFYRDC VSGTIDFIFGDAA 
Sbjct: 361 TAGPQKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTVSGTIDFIFGDAAA 420

Query: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480
           +FQ+C FL+RKPL NQQ IVTAHGRKERRQPSALIIQNC+FK   DL PV+  FRSFLGR
Sbjct: 421 IFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNCSFKPHADLVPVQKQFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540
           PWKEYSRTIIMESYI D+I P+GWLPW+GDWGL+TCFYTE+ NYGPGSD SKRVKWRG+K
Sbjct: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLRTCFYTEYNNYGPGSDKSKRVKWRGIK 540

Query: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLT 575
            +TP HAVDFT  RFL GDRWIK TGVPY  GLT
Sbjct: 541 NITPQHAVDFTPGRFLKGDRWIKPTGVPYVSGLT 573

[Close]

BLAST of CmaCh10G011330 vs. NCBI nr
Match: XP_022968697.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima])

HSP 1 Score: 1171.8 bits (3030), Expect = 0.0e+00
Identity = 581/581 (100.00%), Postives = 581/581 (100.00%), Query Frame = 0


Query: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60
           MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA
Sbjct: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60

Query: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120
           IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP
Sbjct: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120

Query: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180
           RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF
Sbjct: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180

Query: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240
           ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED
Sbjct: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240

Query: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300
           YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV
Sbjct: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300

Query: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360
           YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN
Sbjct: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360

Query: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420
           TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV
Sbjct: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420

Query: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480
           VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR
Sbjct: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540
           PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK
Sbjct: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540

Query: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV 582
           VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV
Sbjct: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV 581

[Close]

BLAST of CmaCh10G011330 vs. NCBI nr
Match: XP_022961544.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata])

HSP 1 Score: 1132.5 bits (2928), Expect = 0.0e+00
Identity = 559/581 (96.21%), Postives = 570/581 (98.11%), Query Frame = 0


Query: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60
           MAYDEDDSG+NKRYAIIGVSSMLL+AMVVAVTVGVNLNN EAGGPT+GTKSNEISASVKA
Sbjct: 1   MAYDEDDSGSNKRYAIIGVSSMLLIAMVVAVTVGVNLNNGEAGGPTTGTKSNEISASVKA 60

Query: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120
           IKSICQ+TDYKKDCVDSLKSNANNS+DPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP
Sbjct: 61  IKSICQSTDYKKDCVDSLKSNANNSSDPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120

Query: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180
           RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDE+LIDLKIWLSATITYQETCLDSF
Sbjct: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEILIDLKIWLSATITYQETCLDSF 180

Query: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240
           ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGH+D
Sbjct: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHDD 240

Query: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300
           YPEWADPGIRRLLAAKG VKANFVVAVDGSGDF +IQSAIDKVPKRKNNETFVIHIKEGV
Sbjct: 241 YPEWADPGIRRLLAAKGKVKANFVVAVDGSGDFNNIQSAIDKVPKRKNNETFVIHIKEGV 300

Query: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360
           YKEYV+IKKSITHLMLIGDGPKKTIISG KNF DGITTFKTAT A TAEHF ARDIGFVN
Sbjct: 301 YKEYVSIKKSITHLMLIGDGPKKTIISGQKNFVDGITTFKTATFAATAEHFLARDIGFVN 360

Query: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420
           TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHT+RQFYRDCIVSGTIDFIFGDAAV
Sbjct: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTLRQFYRDCIVSGTIDFIFGDAAV 420

Query: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480
           VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKN FRSFLGR
Sbjct: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNEFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540
           PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK
Sbjct: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540

Query: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV 582
           VLTPAHAVDFTASRFLLGDRWIKATG PYAPGLTM+ HSEV
Sbjct: 541 VLTPAHAVDFTASRFLLGDRWIKATGTPYAPGLTMKGHSEV 581

[Close]

BLAST of CmaCh10G011330 vs. NCBI nr
Match: KAG6590456.1 (putative pectinesterase/pectinesterase inhibitor 28, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1132.1 bits (2927), Expect = 0.0e+00
Identity = 560/581 (96.39%), Postives = 570/581 (98.11%), Query Frame = 0


Query: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60
           MAYDEDDSG+NKRYAIIGVSSMLLVAMVVAVTVGVNLNN EAGGPT+GTKSNEISASVKA
Sbjct: 1   MAYDEDDSGSNKRYAIIGVSSMLLVAMVVAVTVGVNLNNGEAGGPTTGTKSNEISASVKA 60

Query: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120
           IKSICQ+TDYKKDCVDSLKSNANNS DPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP
Sbjct: 61  IKSICQSTDYKKDCVDSLKSNANNSTDPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120

Query: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180
           RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDE+LIDLKIWLSATITYQETCLDSF
Sbjct: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEILIDLKIWLSATITYQETCLDSF 180

Query: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240
           ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGH+D
Sbjct: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHDD 240

Query: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300
           YPEWADPGIRRLLAAKG VKANFVVAVDGSGDF +IQSAIDKVPKRKNNETFVIHIKEGV
Sbjct: 241 YPEWADPGIRRLLAAKGKVKANFVVAVDGSGDFNNIQSAIDKVPKRKNNETFVIHIKEGV 300

Query: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360
           YKEYV+IKKSITHLMLIGDGPKKTIISG KNF DGITTFKTAT AVTAEHF ARDIGFVN
Sbjct: 301 YKEYVSIKKSITHLMLIGDGPKKTIISGQKNFVDGITTFKTATFAVTAEHFLARDIGFVN 360

Query: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420
           TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHT+RQFYRDCIVSGTIDFIFGDAAV
Sbjct: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTLRQFYRDCIVSGTIDFIFGDAAV 420

Query: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480
           VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKN FRSFLGR
Sbjct: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNEFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540
           PWKEYSRTIIMESYID+VIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK
Sbjct: 481 PWKEYSRTIIMESYIDNVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540

Query: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV 582
           VLTPAHAVDFTASRFLLGD+WIKATG PYAPGLTMR HSEV
Sbjct: 541 VLTPAHAVDFTASRFLLGDKWIKATGTPYAPGLTMRGHSEV 581

[Close]

BLAST of CmaCh10G011330 vs. NCBI nr
Match: XP_023515941.1 (putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1131.3 bits (2925), Expect = 0.0e+00
Identity = 559/581 (96.21%), Postives = 568/581 (97.76%), Query Frame = 0


Query: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60
           MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLN++EAGGPT+GTKSNEISASVKA
Sbjct: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNSEEAGGPTTGTKSNEISASVKA 60

Query: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120
           IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP
Sbjct: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120

Query: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180
           RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDE+LIDLKIWLSATITYQETCLDSF
Sbjct: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEVLIDLKIWLSATITYQETCLDSF 180

Query: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240
           ENTTGDAS+KMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPV VGH+D
Sbjct: 181 ENTTGDASKKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVFVGHDD 240

Query: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300
           YPEWADPGIRRLLAAKG VKANFVVAVDGSGDF SIQSAIDKVPKRKNNETFVIHIKEGV
Sbjct: 241 YPEWADPGIRRLLAAKGKVKANFVVAVDGSGDFNSIQSAIDKVPKRKNNETFVIHIKEGV 300

Query: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360
           YKEYV IKKSITHLML+GDGPKKTIISG KNF DGITTFKTAT A TAEHF ARDIGFVN
Sbjct: 301 YKEYVNIKKSITHLMLVGDGPKKTIISGQKNFVDGITTFKTATFAATAEHFLARDIGFVN 360

Query: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420
           TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHT+RQFYRDCIVSGTIDFIFGDAAV
Sbjct: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTLRQFYRDCIVSGTIDFIFGDAAV 420

Query: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480
           VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKN FRSFLGR
Sbjct: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNEFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540
           PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSD SKRVKWRGVK
Sbjct: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDKSKRVKWRGVK 540

Query: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRRHSEV 582
           VLTPAHAVDFTASRFLLGDRWIKATG PYAPGLTM+ HSEV
Sbjct: 541 VLTPAHAVDFTASRFLLGDRWIKATGTPYAPGLTMKGHSEV 581

[Close]

BLAST of CmaCh10G011330 vs. NCBI nr
Match: XP_038880188.1 (probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida])

HSP 1 Score: 939.5 bits (2427), Expect = 1.4e-269
Identity = 452/577 (78.34%), Postives = 515/577 (89.25%), Query Frame = 0


Query: 1   MAYDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKA 60
           M+Y +DD+ N KRYAIIGVSSMLLVAMVVAVTVGVNLN  E   P  G+KS+EISASVKA
Sbjct: 1   MSYGDDDAQNKKRYAIIGVSSMLLVAMVVAVTVGVNLNQGETSDPAIGSKSHEISASVKA 60

Query: 61  IKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDP 120
           IK+ICQ TDYK++CV SLK+  NNS+DP ELV+AGFKAAMKL+QAAA +SVTL+++E+DP
Sbjct: 61  IKAICQPTDYKQECVASLKAAGNNSSDPKELVQAGFKAAMKLIQAAANKSVTLKEIEKDP 120

Query: 121 RASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSF 180
           R S+ALAGCKELMDFA+DELK+SM++ G+FD + LDEMLID+KIWLSATITYQETCLD F
Sbjct: 121 RTSKALAGCKELMDFAVDELKYSMNKLGEFDISKLDEMLIDIKIWLSATITYQETCLDGF 180

Query: 181 ENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED 240
           ENTTG+A+EKMKKALKTSM+LSSNGLAMV++ISSMLT+L++PGI RRRLL+ P V+GH+D
Sbjct: 181 ENTTGNAAEKMKKALKTSMKLSSNGLAMVSQISSMLTELQIPGISRRRLLEIP-VLGHDD 240

Query: 241 YPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGV 300
           YP+WA+PG+RRLLAA   VK N VVA DGSG F++IQ A+DKVPKRKNN T+VIHIK+GV
Sbjct: 241 YPDWANPGMRRLLAAGTRVKPNVVVAKDGSGQFRTIQEALDKVPKRKNNATYVIHIKQGV 300

Query: 301 YKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVN 360
           YKEY+ IKKS+THLMLIGDGPKKTII+G KNF DG  TFKTATVAVTAEHF ARDIGF N
Sbjct: 301 YKEYLMIKKSLTHLMLIGDGPKKTIITGSKNFIDGTPTFKTATVAVTAEHFMARDIGFEN 360

Query: 361 TAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAV 420
           TAGPHKHQAVALRVQADKA+F+NCEMHGYQDTLYVHTMRQFYRDC +SGTIDF+FGDAA 
Sbjct: 361 TAGPHKHQAVALRVQADKAVFYNCEMHGYQDTLYVHTMRQFYRDCTISGTIDFVFGDAAA 420

Query: 421 VFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGR 480
           VFQ+C FL+RKPL NQQ IVTAHGRKERRQPSALIIQN +FKAD DLAPV+  FRSFLGR
Sbjct: 421 VFQSCTFLVRKPLPNQQCIVTAHGRKERRQPSALIIQNSSFKADADLAPVQKQFRSFLGR 480

Query: 481 PWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVK 540
           PWKEYSRTIIMESYI D+I P+GWLPW+GDWGLKTCFYTE+ NYGPGSD SKRVKWRG+K
Sbjct: 481 PWKEYSRTIIMESYIGDLIQPEGWLPWAGDWGLKTCFYTEYNNYGPGSDKSKRVKWRGIK 540

Query: 541 VLTPAHAVDFTASRFLLGDRWIKATGVPYAPGLTMRR 578
            +TP HAVDFTA RFL GD WIK+TGVPY  G+T  R
Sbjct: 541 TITPQHAVDFTAGRFLRGDSWIKSTGVPYVSGMTRTR 576

[Close]

BLAST of CmaCh10G011330 vs. TAIR 10
Match: AT5G27870.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 628.6 bits (1620), Expect = 4.9e-180
Identity = 304/572 (53.15%), Postives = 411/572 (71.85%), Query Frame = 0


Query: 3   YDEDDSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKAIK 62
           YD++D+   KRY II +SS+LL++MVVAVT+GV++N  +  G        EI+ SVKAIK
Sbjct: 5   YDDEDAKRKKRYVIISISSVLLISMVVAVTIGVSVNKSDNAG------DEEITTSVKAIK 64

Query: 63  SICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQDPRA 122
            +C  TDYK+ C D+L+ +A +++DP ELVK  F A MK +   AK+S T+ +L++DPRA
Sbjct: 65  DVCAPTDYKETCEDTLRKDAKDTSDPLELVKTAFNATMKQISDVAKKSQTMIELQKDPRA 124

Query: 123 SQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSFEN 182
             AL  CKELMD+AI EL  S    G F+   +DE L+ L+IWLSATI++++TCLD F+ 
Sbjct: 125 KMALDQCKELMDYAIGELSKSFEELGKFEFHKVDEALVKLRIWLSATISHEQTCLDGFQG 184

Query: 183 TTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHEDYP 242
           T G+A E +KKALKT+++L+ NGLAMVTE+S+ L  +++P +  RRLL        +++P
Sbjct: 185 TQGNAGETIKKALKTAVQLTHNGLAMVTEMSNYLGQMQIPEMNSRRLLS-------QEFP 244

Query: 243 EWADPGIRRLL-AAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEGVY 302
            W D   RRLL A    VK + VVA DGSG +K+I  A++ VPK+KN  TFV+HIKEG+Y
Sbjct: 245 SWMDARARRLLNAPMSEVKPDIVVAQDGSGQYKTINEALNFVPKKKNT-TFVVHIKEGIY 304

Query: 303 KEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDIGFVNT 362
           KEYV + +S+THL+ IGDGP KT+ISG K++ DGITT+KTATVA+  +HF A++I F NT
Sbjct: 305 KEYVQVNRSMTHLVFIGDGPDKTVISGSKSYKDGITTYKTATVAIVGDHFIAKNIAFENT 364

Query: 363 AGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDAAVV 422
           AG  KHQAVA+RV AD++IF+NC+  GYQDTLY H+ RQFYRDC +SGTIDF+FGDAA V
Sbjct: 365 AGAIKHQAVAIRVLADESIFYNCKFDGYQDTLYAHSHRQFYRDCTISGTIDFLFGDAAAV 424

Query: 423 FQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFLGRP 482
           FQNC  L+RKPL NQ   +TAHGRK+ R+ +  ++Q CT   + D   VK   +++LGRP
Sbjct: 425 FQNCTLLVRKPLLNQACPITAHGRKDPRESTGFVLQGCTIVGEPDYLAVKEQSKTYLGRP 484

Query: 483 WKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRGVKV 542
           WKEYSRTIIM ++I D + P+GW PW G++GL T FY+E +N GPG+  +KRV W G+K 
Sbjct: 485 WKEYSRTIIMNTFIPDFVPPEGWQPWLGEFGLNTLFYSEVQNTGPGAAITKRVTWPGIKK 544

Query: 543 LTPAHAVDFTASRFLLGDRWIKATGVPYAPGL 574
           L+    + FT ++++ GD WI   GVPY  GL
Sbjct: 545 LSDEEILKFTPAQYIQGDAWIPGKGVPYILGL 562

[Close]

BLAST of CmaCh10G011330 vs. TAIR 10
Match: AT3G05610.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 620.2 bits (1598), Expect = 1.7e-177
Identity = 306/577 (53.03%), Postives = 406/577 (70.36%), Query Frame = 0


Query: 1   MAYDEDDSGNNK-RYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVK 60
           M+Y  DD    K RY +I +SS+LL++MVVAVTVGV+LN  +  G + G    E++ASVK
Sbjct: 1   MSYGYDDESKRKRRYIVITISSVLLISMVVAVTVGVSLNKHD--GDSKG--KAEVNASVK 60

Query: 61  AIKSICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSVTLQQLEQD 120
           A+K +C  TDY+K C D+L  N  N+ DP ELVK  F   MK +  AAK+S T+ +L++D
Sbjct: 61  AVKDVCAPTDYRKTCEDTLIKNGKNTTDPMELVKTAFNVTMKQITDAAKKSQTIMELQKD 120

Query: 121 PRASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDS 180
            R   AL  CKELMD+A+DEL  S    G F+   LDE LI+L+IWLSA I+++ETCL+ 
Sbjct: 121 SRTRMALDQCKELMDYALDELSNSFEELGKFEFHLLDEALINLRIWLSAAISHEETCLEG 180

Query: 181 FENTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHE 240
           F+ T G+A E MKKALKT++ L+ NGLA+++E+S+ +  +++PG+  RRLL        E
Sbjct: 181 FQGTQGNAGETMKKALKTAIELTHNGLAIISEMSNFVGQMQIPGLNSRRLL-------AE 240

Query: 241 DYPEWADPGIRRLL---AAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHI 300
            +P W D   R+LL   AA  +VK + VVA DGSG +K+I  A+  VPK K N TFV+HI
Sbjct: 241 GFPSWVDQRGRKLLQAAAAYSDVKPDIVVAQDGSGQYKTINEALQFVPK-KRNTTFVVHI 300

Query: 301 KEGVYKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDGITTFKTATVAVTAEHFRARDI 360
           K G+YKEYV + K+++HL+ IGDGP KTIISG+KN+ DGITT++TATVA+   +F A++I
Sbjct: 301 KAGLYKEYVQVNKTMSHLVFIGDGPDKTIISGNKNYKDGITTYRTATVAIVGNYFIAKNI 360

Query: 361 GFVNTAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFG 420
           GF NTAG  KHQAVA+RVQ+D++IFFNC   GYQDTLY H+ RQF+RDC +SGTIDF+FG
Sbjct: 361 GFENTAGAIKHQAVAVRVQSDESIFFNCRFDGYQDTLYTHSHRQFFRDCTISGTIDFLFG 420

Query: 421 DAAVVFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRS 480
           DAA VFQNC  L+RKPL NQ   +TAHGRK+ R+ +  + Q CT   + D   VK   ++
Sbjct: 421 DAAAVFQNCTLLVRKPLPNQACPITAHGRKDPRESTGFVFQGCTIAGEPDYLAVKETSKA 480

Query: 481 FLGRPWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKW 540
           +LGRPWKEYSRTIIM ++I D + P GW PW GD+GLKT FY+E +N GPGS  + RV W
Sbjct: 481 YLGRPWKEYSRTIIMNTFIPDFVQPQGWQPWLGDFGLKTLFYSEVQNTGPGSALANRVTW 540

Query: 541 RGVKVLTPAHAVDFTASRFLLGDRWIKATGVPYAPGL 574
            G+K L+    + FT ++++ GD WI   GVPY  GL
Sbjct: 541 AGIKTLSEEDILKFTPAQYIQGDDWIPGKGVPYTTGL 565

[Close]

BLAST of CmaCh10G011330 vs. TAIR 10
Match: AT5G49180.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 525.8 bits (1353), Expect = 4.5e-149
Identity = 269/575 (46.78%), Postives = 377/575 (65.57%), Query Frame = 0


Query: 6   DDSGNNKRYAIIGVSSMLLVAMVVAV--TVGVNLNNDEAGGPTSGTKSNEISASVKAIKS 65
           D     K+  I GV + LLV MVVAV  T   N ++ E   P       +I  +  A+++
Sbjct: 4   DGELKKKKCIIAGVITALLVLMVVAVGITTSRNTSHSEKIVPV------QIKTATTAVEA 63

Query: 66  ICQTTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQ-SVTL-QQLEQDPR 125
           +C  TDYK+ CV+SL   + +S  P +L+K GF   ++ ++ + K+ SV L  +   D  
Sbjct: 64  VCAPTDYKETCVNSLMKASPDSTQPLDLIKLGFNVTIRSIEDSIKKASVELTAKAANDKD 123

Query: 126 ASQALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSFE 185
              AL  C++LM+ A D+LK  +  F  F    +++ + DL++WLS +I YQ+TC+D+FE
Sbjct: 124 TKGALELCEKLMNDATDDLKKCLDNFDGFSIPQIEDFVEDLRVWLSGSIAYQQTCMDTFE 183

Query: 186 NTTGDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIG------RRRLLDAPVV 245
            T    S+ M+K  KTS  L+SNGLAM+T IS++L +  V G+        R+LL A   
Sbjct: 184 ETNSKLSQDMQKIFKTSRELTSNGLAMITNISNLLGEFNVTGVTGDLGKYARKLLSA--- 243

Query: 246 VGHEDYPEWADPGIRRLLAAKGNVKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIH 305
              +  P W  P  RRL+A KG VKAN VVA DGSG +K+I  A++ VPK  N + FVI+
Sbjct: 244 --EDGIPSWVGPNTRRLMATKGGVKANVVVAHDGSGQYKTINEALNAVPK-ANQKPFVIY 303

Query: 306 IKEGVYKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDG-ITTFKTATVAVTAEHFRAR 365
           IK+GVY E V + K +TH+  IGDGP KT I+G  N++ G + T+ TATVA+  ++F A+
Sbjct: 304 IKQGVYNEKVDVTKKMTHVTFIGDGPTKTKITGSLNYYIGKVKTYLTATVAINGDNFTAK 363

Query: 366 DIGFVNTAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFI 425
           +IGF NTAGP  HQAVALRV AD A+F+NC++ GYQDTLYVH+ RQF+RDC VSGT+DFI
Sbjct: 364 NIGFENTAGPEGHQAVALRVSADLAVFYNCQIDGYQDTLYVHSHRQFFRDCTVSGTVDFI 423

Query: 426 FGDAAVVFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVF 485
           FGD  VV QNC  ++RKP+ +Q  ++TA GR ++R+ + L++QNC    +    PVK++ 
Sbjct: 424 FGDGIVVLQNCNIVVRKPMKSQSCMITAQGRSDKRESTGLVLQNCHITGEPAYIPVKSIN 483

Query: 486 RSFLGRPWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRV 545
           +++LGRPWKE+SRTIIM + IDDVI P GWLPW+GD+ L T +Y E+ N GPGS+ ++RV
Sbjct: 484 KAYLGRPWKEFSRTIIMGTTIDDVIDPAGWLPWNGDFALNTLYYAEYENNGPGSNQAQRV 543

Query: 546 KWRGVKVLTPAHAVDFTASRFLLGDRWIKATGVPY 570
           KW G+K L+P  A+ FT +RFL G+ WI    VPY
Sbjct: 544 KWPGIKKLSPKQALRFTPARFLRGNLWIPPNRVPY 566

[Close]

BLAST of CmaCh10G011330 vs. TAIR 10
Match: AT3G06830.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 496.5 bits (1277), Expect = 2.9e-140
Identity = 263/571 (46.06%), Postives = 364/571 (63.75%), Query Frame = 0


Query: 7   DSGNNKRYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKAIKSICQ 66
           D    K++ + G  S  LV MVV+V V  + ++ +          N I  + KA++++C 
Sbjct: 4   DGDKKKKFIVAGSVSGFLVIMVVSVAVVTSKHSPK-------DDENHIRKTTKAVQAVCA 63

Query: 67  TTDYKKDCVDSLKSNANNSADPHELVKAGFKAAMKLL-QAAAKQSVTLQ-QLEQDPRASQ 126
            TD+K  CV+SL   + +S DP +L+K GFK  +K + ++  K S  ++ + +++P A  
Sbjct: 64  PTDFKDTCVNSLMGASPDSDDPVDLIKLGFKVTIKSINESLEKASGDIKAKADKNPEAKG 123

Query: 127 ALAGCKELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSFENTT 186
           A   C++LM  AID+LK  M     F    ++  + DL++WLS +I +Q+TC+DSF    
Sbjct: 124 AFELCEKLMIDAIDDLKKCMDH--GFSVDQIEVFVEDLRVWLSGSIAFQQTCMDSFGEIK 183

Query: 187 GDASEKMKKALKTSMRLSSNGLAMVTEISSMLTDLKVPGIGRRRLLDAPVVVGHED-YPE 246
            +  + M K  KTS  LSSN LAMVT IS+++ +  + G+       A  ++  ED  P 
Sbjct: 184 SNLMQDMLKIFKTSRELSSNSLAMVTRISTLIPNSNLTGLTGALAKYARKLLSTEDSIPT 243

Query: 247 WADPGIRRLLAAKGN----VKANFVVAVDGSGDFKSIQSAIDKVPKRKNNETFVIHIKEG 306
           W  P  RRL+AA+G     VKAN VVA DG+G FK+I  A++ VPK  N   F+IHIKEG
Sbjct: 244 WVGPEARRLMAAQGGGPGPVKANAVVAQDGTGQFKTITDALNAVPK-GNKVPFIIHIKEG 303

Query: 307 VYKEYVTIKKSITHLMLIGDGPKKTIISGHKNFFDG-ITTFKTATVAVTAEHFRARDIGF 366
           +YKE VT+ K + H+  IGDGP KT+I+G  NF  G + TF TAT+ +  +HF A++IG 
Sbjct: 304 IYKEKVTVTKKMPHVTFIGDGPNKTLITGSLNFGIGKVKTFLTATITIEGDHFTAKNIGI 363

Query: 367 VNTAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIVSGTIDFIFGDA 426
            NTAGP   QAVALRV AD A+F +C++ G+QDTLYVH+ RQFYRDC VSGT+DFIFGDA
Sbjct: 364 ENTAGPEGGQAVALRVSADYAVFHSCQIDGHQDTLYVHSHRQFYRDCTVSGTVDFIFGDA 423

Query: 427 AVVFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDLAPVKNVFRSFL 486
             + QNC  ++RKP   Q  +VTA GR   R+ + L++  C    D    P+K+V +++L
Sbjct: 424 KCILQNCKIVVRKPNKGQTCMVTAQGRSNVRESTGLVLHGCHITGDPAYIPMKSVNKAYL 483

Query: 487 GRPWKEYSRTIIMESYIDDVIHPDGWLPWSGDWGLKTCFYTEFRNYGPGSDTSKRVKWRG 546
           GRPWKE+SRTIIM++ IDDVI P GWLPWSGD+ LKT +Y E  N GPGS+ ++RVKW G
Sbjct: 484 GRPWKEFSRTIIMKTTIDDVIDPAGWLPWSGDFALKTLYYAEHMNTGPGSNQAQRVKWPG 543

Query: 547 VKVLTPAHAVDFTASRFLLGDRWIKATGVPY 570
           +K LTP  A+ +T  RFL GD WI  T VPY
Sbjct: 544 IKKLTPQDALLYTGDRFLRGDTWIPQTQVPY 564

[Close]

BLAST of CmaCh10G011330 vs. TAIR 10
Match: AT4G15980.1 (Plant invertase/pectin methylesterase inhibitor superfamily )

HSP 1 Score: 440.7 bits (1132), Expect = 1.9e-123
Identity = 261/702 (37.18%), Postives = 367/702 (52.28%), Query Frame = 0


Query: 13  RYAIIGVSSMLLVAMVVAVTVGVNLNNDEAGGPTSGTKSNEISASVKAIKSICQTTDYKK 72
           +Y ++GV++ L++AMV+ V    N  N  +       + +++  +   +  IC +TDYK+
Sbjct: 3   KYVLLGVTA-LIMAMVICVE--ANDGNSPSRKMEEVHRESKLMITKTTVSIICASTDYKQ 62

Query: 73  DCVDSLKSNANNSADPHELVKAGFKAAMKLLQAAAKQSV--TLQQLEQDPRASQALAGCK 132
           DC  SL +    S DP  L+++ F  A+  +++   + +     + + D    +AL  C+
Sbjct: 63  DCTTSLAT--VRSPDPRNLIRSAFDLAIISIRSGIDRGMIDLKSRADADMHTREALNTCR 122

Query: 133 ELMDFAIDELKFSMSRFGDFDTTHLDEMLIDLKIWLSATITYQETCLDSFENTTGDASEK 192
           ELMD AID+L+ +  +F  F  T L + + DL +WLS +ITYQ+TC+D FE    +A+  
Sbjct: 123 ELMDDAIDDLRKTRDKFRGFLFTRLSDFVEDLCVWLSGSITYQQTCIDGFEGIDSEAAVM 182

Query: 193 MKKALKTSMRLSSNGLAMVTEISSMLTDLKVP------GIGRRRLL-------------- 252
           M++ ++    L+SNGLA+   +  +L   ++P        GR  +L              
Sbjct: 183 MERVMRKGQHLTSNGLAIAANLDKLLKAFRIPFPFLRSRRGRLGILGSGSSRDESVGSSQ 242

Query: 253 ----------DAPVVV-------------------------------------------- 312
                     D+P  V                                            
Sbjct: 243 DSPPNEDSSDDSPSTVDSSENQPVDSSSENQSSDSSNNRPLDSSKNQQMESSEDTPKKSA 302

Query: 313 --GHE------------------------------------------------------- 372
             G++                                                       
Sbjct: 303 FSGNQPLDDSSDKLPQKSTSSENQPLDSSENPPQKSTSSENRPLDPLRKLNPLNKLDSLK 362

Query: 373 --------DYPEWADPGIRRLLAAK---GNVKANFVVAVDGSGDFKSIQSAIDKVPKRKN 432
                   ++P W  P  RRLLA +     +KAN VVA DGSG  K+I  A+  VP  KN
Sbjct: 363 DRHLSEEGEFPPWVTPHSRRLLARRPRNNGIKANVVVAKDGSGKCKTIAQALAMVP-MKN 422

Query: 433 NETFVIHIKEGVYKEYVTIKKSITHLMLIGDGPKKTIISGHKNFF-DGITTFKTATVAVT 492
            + FVIHIKEGVYKE V + K + H+M +GDGP KT+I+G   F  D + T++TA+VAV 
Sbjct: 423 TKKFVIHIKEGVYKEKVEVTKKMLHVMFVGDGPTKTVITGDIAFLPDQVGTYRTASVAVN 482

Query: 493 AEHFRARDIGFVNTAGPHKHQAVALRVQADKAIFFNCEMHGYQDTLYVHTMRQFYRDCIV 552
            ++F A+DIGF NTAG  +HQAVALRV AD A+FFNC M+GYQDTLYVHT RQFYR+C V
Sbjct: 483 GDYFMAKDIGFENTAGAARHQAVALRVSADFAVFFNCHMNGYQDTLYVHTHRQFYRNCRV 542

Query: 553 SGTIDFIFGDAAVVFQNCIFLIRKPLSNQQNIVTAHGRKERRQPSALIIQNCTFKADTDL 570
           SGTIDF+FGDA  VFQNC F+IR+P+ +QQ IVTA GRK+RR+ + ++I N     D   
Sbjct: 543 SGTIDFVFGDAKAVFQNCEFVIRRPMEHQQCIVTAQGRKDRRETTGIVIHNSRITGDASY 602

The following BLAST results are available for this feature:

BLAST of CmaCh10G011330 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q3E8Z8	6.9e-179	53.15	Putative pectinesterase/pectinesterase inhibitor 28 OS=Arabidopsis thaliana OX=3...	[more]
Q8GX86	2.4e-176	53.03	Probable pectinesterase/pectinesterase inhibitor 21 OS=Arabidopsis thaliana OX=3...	[more]
Q9FJ21	6.3e-148	46.78	Probable pectinesterase/pectinesterase inhibitor 58 OS=Arabidopsis thaliana OX=3...	[more]
Q8GXA1	4.1e-139	46.06	Probable pectinesterase/pectinesterase inhibitor 23 OS=Arabidopsis thaliana OX=3...	[more]
O23447	2.7e-122	37.18	Putative pectinesterase/pectinesterase inhibitor 43 OS=Arabidopsis thaliana OX=3...	[more]

BLAST of CmaCh10G011330 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1HU87	0.0e+00	100.00	Pectinesterase OS=Cucurbita maxima OX=3661 GN=LOC111467859 PE=3 SV=1	[more]
A0A6J1HEC9	0.0e+00	96.21	Pectinesterase OS=Cucurbita moschata OX=3662 GN=LOC111462093 PE=3 SV=1	[more]
A0A5D3CGC3	1.1e-267	77.53	Pectinesterase OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G004180...	[more]
A0A1S3BPI8	1.1e-267	77.53	Pectinesterase OS=Cucumis melo OX=3656 GN=LOC103492141 PE=3 SV=1	[more]
A0A0A0LYF5	6.9e-267	78.05	Pectinesterase OS=Cucumis sativus OX=3659 GN=Csa_1G569220 PE=3 SV=1	[more]

BLAST of CmaCh10G011330 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022968697.1	0.0e+00	100.00	putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita maxima]	[more]
XP_022961544.1	0.0e+00	96.21	putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita moschata]	[more]
KAG6590456.1	0.0e+00	96.39	putative pectinesterase/pectinesterase inhibitor 28, partial [Cucurbita argyrosp...	[more]
XP_023515941.1	0.0e+00	96.21	putative pectinesterase/pectinesterase inhibitor 28 [Cucurbita pepo subsp. pepo]	[more]
XP_038880188.1	1.4e-269	78.34	probable pectinesterase/pectinesterase inhibitor 21 [Benincasa hispida]	[more]

BLAST of CmaCh10G011330 vs. TAIR 10


Match Name	E-value	Identity	Description
AT5G27870.1	4.9e-180	53.15	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G05610.1	1.7e-177	53.03	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT5G49180.1	4.5e-149	46.78	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT3G06830.1	2.9e-140	46.06	Plant invertase/pectin methylesterase inhibitor superfamily	[more]
AT4G15980.1	1.9e-123	37.18	Plant invertase/pectin methylesterase inhibitor superfamily	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR006501	Pectinesterase inhibitor domain	SMART	SM00856	PMEI_2	coord: 56..208 e-value: 8.3E-33 score: 125.0
IPR006501	Pectinesterase inhibitor domain	TIGRFAM	TIGR01614	TIGR01614	coord: 54..211 e-value: 5.1E-24 score: 82.5
IPR006501	Pectinesterase inhibitor domain	PFAM	PF04043	PMEI	coord: 60..208 e-value: 2.5E-25 score: 89.6
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	GENE3D	1.20.140.40	Invertase/pectin methylesterase inhibitor family protein	coord: 56..213 e-value: 1.7E-32 score: 114.5
IPR035513	Invertase/pectin methylesterase inhibitor domain superfamily	SUPERFAMILY	101148	Plant invertase/pectin methylesterase inhibitor	coord: 59..213
IPR000070	Pectinesterase, catalytic	PFAM	PF01095	Pectinesterase	coord: 263..559 e-value: 3.5E-136 score: 452.8
IPR012334	Pectin lyase fold	GENE3D	2.160.20.10		coord: 256..573 e-value: 8.1E-118 score: 395.4
None	No IPR available	PANTHER	PTHR31707:SF131	PECTINESTERASE	coord: 16..574
None	No IPR available	PANTHER	PTHR31707	PECTINESTERASE	coord: 16..574
None	No IPR available	CDD	cd15798	PMEI-like_3	coord: 63..214 e-value: 1.45343E-41 score: 145.274
IPR033131	Pectinesterase, Asp active site	PROSITE	PS00503	PECTINESTERASE_2	coord: 407..416
IPR018040	Pectinesterase, Tyr active site	PROSITE	PS00800	PECTINESTERASE_1	coord: 288..307
IPR011050	Pectin lyase fold/virulence factor	SUPERFAMILY	51126	Pectin lyase-like	coord: 260..572

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh10G011330.1	CmaCh10G011330.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0042545	cell wall modification
biological_process	GO:0043086	negative regulation of catalytic activity
biological_process	GO:0045490	pectin catabolic process
cellular_component	GO:0005576	extracellular region
cellular_component	GO:0016021	integral component of membrane
molecular_function	GO:0045330	aspartyl esterase activity
molecular_function	GO:0030599	pectinesterase activity
molecular_function	GO:0046910	pectinesterase inhibitor activity
molecular_function	GO:0004857	enzyme inhibitor activity