CmaCh10G008610 (gene) Cucurbita maxima (Rimu) v1.1

Overview


Name	CmaCh10G008610
Type	gene
Organism	Cucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Description	RING-type E3 ubiquitin transferase
Location	Cma_Chr10: 4205810 .. 4207604 (+)
RNA-Seq Expression	CmaCh10G008610
Synteny	CmaCh10G008610

Sequences

The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide

Hold the cursor over a type above to highlight its positions in the sequence below.

ATGGCCGAAATCGAGGTTCCTTCCGACTTTCTATGCCCGATTTCTCTACAAATCATGAGCGACCCAGTGACGATATCGACCGGGATCACATACGATAGAGAGAGCATAGAGAAATGGCTCTTGTTTTGCAAGAAATGCCCGGTGACAAAGCAGCCACTGATCGGTATAGATTTGACGCCGAACCATACGCTCCGTCGGGTGATTCAAGGGTGGAGCTGCGTCAACACCGAGCACGGGGTGGAGCGGGTCCGAAGCCCTAAGGGTGAGGGTGATGGAGGGCATGTTGTTAAGCTGATCCTTAAGGAGGCAATGAGAGGTAGAAGCTCAAGGCTTGAGTGCCTGAACAAATTGGAAGCTTTCTTGGCTGAGACTGAGAGGAACAAAATCTACTTGCAGGCAAGCATTTATACTATTTAATTTTTTTTTTTTTTTTTTAAATTAAAAAATCTTATTTTTCTCCGATAATATTTAAAGGATTAGGGTTAGATCCACGGGATTGGAGAGGGAAACGAAACATCTTTATAAGAGTGTGCTAACCTTTCAGATTTTAAAACCGTGAGACTGACGATGATACATAACGTGCCAAACTGAACAATATTTGTTGGCTGTGGGCTTGAGTTGTTATAGTTAGCCTAATTTCAAAAATAAAAACCATTCTCGATTTCAAAAAAACTTATTTTCCGACCATATATTTTAATTATATTTTATTTTTGAAATAAGATGGTATAATTATTTTATTGTATTAAATAAAAGTTTATATAATTTTATTAAAATAATTTATTTGAGGATAATAATTAAGATTTATTACTAGTTGTATAACTTGACTCGTGCTATCTACTTGAATAGTATTCGTTTGTTTATCGAGTTTGAGCTACAAATGGTCTATGTCTTCTTGTACGTTTAACAGTTTAACACACAATTACTGTTTACACAACAATCATCTACTATCGACTCGGTAATGAAACATTATGATCGATAATATGTTTAGGATGCTCGATCCATGGAGTTCTTAACTTCAATGTTGAACAATCCCGAGGACGAGAATGAGGAAGTCATTGTAGAAAAAGCTATAGAAATCATTTTGAACATCAATTCACCCACTACTCTCCTCGAACATCTCTTAATCCAAAACGCTAACCTCATTGATGCCGTAATCTCCATCGTTGCCGTAACCAAAACCTCGAGATCTCGAGGGGCCGCCATAGCCTTCCTAAGCTCCCTCTACTCCATTTCCGACCAAACCCACAAAATCTTCACAAAAGACAACCTCTTCATCCAACTCTCAAGAGCCTTAAACGACCAAATCGCCATAAAACCCGCCCTTAAAATCCTCCTCCACTTGGCCCCATTTGGTCGGAACCGTATCAAAGCCGTGAAACACGGTGTCGTTTTCGACCTCGTCGAGCTGCTTTTGAACTCCACCGTTGGAAGAGAATGCGAGCTGGCAATTATGGCCATGGACCATCTTTGTGAATGTGCCGAAGGCCGGGCTGAGCTGCTCCGACATGGAGGCGGGATGGCGGTCGTGGCGAGGAAGATTCTTCGAGTGTCGAATTTTAGCAACGAGAAGGCGGTTAGGATTTTGTACCATGTTTGTAAGAACAACGTTGGGAATTTCGGAGTTGCTGAGGAAATGGTGGAGGTTGGGGCTGTTGGGAAGCTTTGTTTGATGCTTCAAATTGGTGGAAGTTTGAAAACTAATGAAAGAATTAAGGAGATTCTTCATTTTCTTCGATGTATTTTCAAGGGTACGAAATGTGTTCTTGTTCTTCCGTCCGGGTTTGATCGGTTTTAG

mRNA sequence

ATGGCCGAAATCGAGGTTCCTTCCGACTTTCTATGCCCGATTTCTCTACAAATCATGAGCGACCCAGTGACGATATCGACCGGGATCACATACGATAGAGAGAGCATAGAGAAATGGCTCTTGTTTTGCAAGAAATGCCCGGTGACAAAGCAGCCACTGATCGGTATAGATTTGACGCCGAACCATACGCTCCGTCGGGTGATTCAAGGGTGGAGCTGCGTCAACACCGAGCACGGGGTGGAGCGGGTCCGAAGCCCTAAGGGTGAGGGTGATGGAGGGCATGTTGTTAAGCTGATCCTTAAGGAGGCAATGAGAGGTAGAAGCTCAAGGCTTGAGTGCCTGAACAAATTGGAAGCTTTCTTGGCTGAGACTGAGAGGAACAAAATCTACTTGCAGGATGCTCGATCCATGGAGTTCTTAACTTCAATGTTGAACAATCCCGAGGACGAGAATGAGGAAGTCATTGTAGAAAAAGCTATAGAAATCATTTTGAACATCAATTCACCCACTACTCTCCTCGAACATCTCTTAATCCAAAACGCTAACCTCATTGATGCCGTAATCTCCATCGTTGCCGTAACCAAAACCTCGAGATCTCGAGGGGCCGCCATAGCCTTCCTAAGCTCCCTCTACTCCATTTCCGACCAAACCCACAAAATCTTCACAAAAGACAACCTCTTCATCCAACTCTCAAGAGCCTTAAACGACCAAATCGCCATAAAACCCGCCCTTAAAATCCTCCTCCACTTGGCCCCATTTGGTCGGAACCGTATCAAAGCCGTGAAACACGGTGTCGTTTTCGACCTCGTCGAGCTGCTTTTGAACTCCACCGTTGGAAGAGAATGCGAGCTGGCAATTATGGCCATGGACCATCTTTGTGAATGTGCCGAAGGCCGGGCTGAGCTGCTCCGACATGGAGGCGGGATGGCGGTCGTGGCGAGGAAGATTCTTCGAGTGTCGAATTTTAGCAACGAGAAGGCGGTTAGGATTTTGTACCATGTTTGTAAGAACAACGTTGGGAATTTCGGAGTTGCTGAGGAAATGGTGGAGGTTGGGGCTGTTGGGAAGCTTTGTTTGATGCTTCAAATTGGTGGAAGTTTGAAAACTAATGAAAGAATTAAGGAGATTCTTCATTTTCTTCGATGTATTTTCAAGGGTACGAAATGTGTTCTTGTTCTTCCGTCCGGGTTTGATCGGTTTTAG

Coding sequence (CDS)

ATGGCCGAAATCGAGGTTCCTTCCGACTTTCTATGCCCGATTTCTCTACAAATCATGAGCGACCCAGTGACGATATCGACCGGGATCACATACGATAGAGAGAGCATAGAGAAATGGCTCTTGTTTTGCAAGAAATGCCCGGTGACAAAGCAGCCACTGATCGGTATAGATTTGACGCCGAACCATACGCTCCGTCGGGTGATTCAAGGGTGGAGCTGCGTCAACACCGAGCACGGGGTGGAGCGGGTCCGAAGCCCTAAGGGTGAGGGTGATGGAGGGCATGTTGTTAAGCTGATCCTTAAGGAGGCAATGAGAGGTAGAAGCTCAAGGCTTGAGTGCCTGAACAAATTGGAAGCTTTCTTGGCTGAGACTGAGAGGAACAAAATCTACTTGCAGGATGCTCGATCCATGGAGTTCTTAACTTCAATGTTGAACAATCCCGAGGACGAGAATGAGGAAGTCATTGTAGAAAAAGCTATAGAAATCATTTTGAACATCAATTCACCCACTACTCTCCTCGAACATCTCTTAATCCAAAACGCTAACCTCATTGATGCCGTAATCTCCATCGTTGCCGTAACCAAAACCTCGAGATCTCGAGGGGCCGCCATAGCCTTCCTAAGCTCCCTCTACTCCATTTCCGACCAAACCCACAAAATCTTCACAAAAGACAACCTCTTCATCCAACTCTCAAGAGCCTTAAACGACCAAATCGCCATAAAACCCGCCCTTAAAATCCTCCTCCACTTGGCCCCATTTGGTCGGAACCGTATCAAAGCCGTGAAACACGGTGTCGTTTTCGACCTCGTCGAGCTGCTTTTGAACTCCACCGTTGGAAGAGAATGCGAGCTGGCAATTATGGCCATGGACCATCTTTGTGAATGTGCCGAAGGCCGGGCTGAGCTGCTCCGACATGGAGGCGGGATGGCGGTCGTGGCGAGGAAGATTCTTCGAGTGTCGAATTTTAGCAACGAGAAGGCGGTTAGGATTTTGTACCATGTTTGTAAGAACAACGTTGGGAATTTCGGAGTTGCTGAGGAAATGGTGGAGGTTGGGGCTGTTGGGAAGCTTTGTTTGATGCTTCAAATTGGTGGAAGTTTGAAAACTAATGAAAGAATTAAGGAGATTCTTCATTTTCTTCGATGTATTTTCAAGGGTACGAAATGTGTTCTTGTTCTTCCGTCCGGGTTTGATCGGTTTTAG

Protein sequence

MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTPNHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAFLAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF

Homology

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BLAST of CmaCh10G008610 vs. ExPASy Swiss-Prot
Match: Q9SVC6 (E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 SV=1)

HSP 1 Score: 271.6 bits (693), Expect = 1.5e-71
Identity = 166/429 (38.69%), Postives = 251/429 (58.51%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKK--CPVTKQPLIGIDLTP 62
           EIE+PS FLCPISL IM DPV +STGITYDRESIEKWL   KK  CPVTKQ +   DLTP
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 63  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 122
           NHTLRR+IQ W  +N  +G+ER+ +PK       + KLI KE+     ++++CL +L   
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLI-KESSSSHLNQVKCLKRLRQI 123

Query: 123 LAETERNKIYLQDARSMEFLTSMLNNPED---------------------------ENEE 182
           ++E   NK  L+ A   EFL ++++N  D                           ++  
Sbjct: 124 VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 183

Query: 183 VIVEKAIEIILNINSPTTLLEHLL--IQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLY 242
            ++++A+ ++ ++++  T L+ LL   +  NL+  +  I+       SR  A   L  L 
Sbjct: 184 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQ-RGIYESRAYAALLLKKLL 243

Query: 243 SISDQTHKIFTKDNLFIQLSRALNDQI---AIKPALKILLHLAPFGRNRIKAVKHGVVFD 302
            ++D    I  +  LF ++ + L+DQI   A + A++IL+   P+GRNR KAV+ G +  
Sbjct: 244 EVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISM 303

Query: 303 LVELLLNSTVG---RECELAIMAMDHLCECAEGRAELLRHGGGMAVVARKILRVSNFSNE 362
           ++ELL++ T     R  E+A++ +D LC+CAEGRAE L HG  +AVV++KILRVS  ++E
Sbjct: 304 IIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSE 363

Query: 363 KAVRILYHVCKNNVGNF----GVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEILHFLR 391
           +AVR+L      +VG F     + +EM+++G V KLCL+LQ+    KT E+ KE+L    
Sbjct: 364 RAVRVLL-----SVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHA 423

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BLAST of CmaCh10G008610 vs. ExPASy Swiss-Prot
Match: Q84TG3 (E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 SV=1)

HSP 1 Score: 271.2 bits (692), Expect = 1.9e-71
Identity = 158/402 (39.30%), Postives = 252/402 (62.69%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKK--CPVTKQPLIGIDLTP 62
           EIE+P  FLCPISL+IM DPV +STGITYDR+SIEKWL   KK  CPVTKQ +   DLTP
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 63  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 122
           NHTLRR+IQ W  +N  +GVER+ +P+       + KLI +++     ++++CL +L   
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLI-RDSASSHENQVKCLKRLRQI 128

Query: 123 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLL--I 182
           ++E   NK  L+ A   EFL ++++N  D     + ++A+ ++ ++ +  T+L++LL   
Sbjct: 129 VSENATNKRCLEAAGVPEFLANIVSN--DSENGSLTDEALNLLYHLETSETVLKNLLNNK 188

Query: 183 QNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQI 242
           ++ N++ ++  I+       SR  A   L ++  ++D    +  K  +F ++ + L+D+I
Sbjct: 189 KDNNIVKSLTKIMQ-RGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRI 248

Query: 243 ---AIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRE---CELAIMAMDHL 302
              A K A+ IL+++ P+GRNR KAV+ GV+  ++ELL++ +   E    E+A++ +D L
Sbjct: 249 SQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLL 308

Query: 303 CECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNF----GVAEEM 362
           C+CAEGRAE L HG  +AVV +KILRVS  ++++AVR+L      +VG F     +  EM
Sbjct: 309 CQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLL-----SVGRFCATPALLHEM 368

Query: 363 VEVGAVGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCV 391
           +++G V KLCL+LQ+    KT E+ KE+L     ++K + C+
Sbjct: 369 LQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

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BLAST of CmaCh10G008610 vs. ExPASy Swiss-Prot
Match: Q9SF15 (E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 SV=1)

HSP 1 Score: 184.9 bits (468), Expect = 1.8e-45
Identity = 145/425 (34.12%), Postives = 220/425 (51.76%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPL-IGIDLTPN 62
           EIE+P+ F+CPISL+IM DPVT  +GITYDR++I KWL     CPVTKQPL +  DLTPN
Sbjct: 7   EIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKVPSCPVTKQPLPLDSDLTPN 66

Query: 63  HTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLI--LKEAMRGRSSRLECLNKLEA 122
           H LRR+IQ W   N   GV R+ +P+      +VV+ I  LK+  +    R E L KLE 
Sbjct: 67  HMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLEV 126

Query: 123 FLAETERNKIYLQ---DARSMEFLTSMLNNPEDENEEVI-VEKAIEIILNINSPTTLLEH 182
              +    ++  +       + F+    +  ED    +  +++++ ++  I  P+   + 
Sbjct: 127 LAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSNDAKT 186

Query: 183 LLIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSL--YSISDQTHKI----------FTK 242
           +L++N  ++++ ++ V   +   S+   I  L +L  Y+ S    ++          F K
Sbjct: 187 ILMENDRVMES-LTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKGIIGFLK 246

Query: 243 D--NLFIQLSRALNDQI------------------------AIKPALKILLHLAPFGRNR 302
           D  N   + S  + + +                        A+  AL ILL  + + RNR
Sbjct: 247 DVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSWSRNR 306

Query: 303 IKAVKHGVVFDLVELLLNST-VGRECELAIMAMDHLCECAEGRAELLRHGGGMAVVARKI 362
              V  G V +L+EL ++ T   R  EL +  +  LC CA GRAE+L H GG+AVV +++
Sbjct: 307 SLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVVTKRL 366

Query: 363 LRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEI 380
           LRVS  ++++A+ IL  V K +  N  V EEMV VG V KLC +L +   L   E+ KEI
Sbjct: 367 LRVSPAADDRAISILTTVSKFSPENM-VVEEMVNVGTVEKLCSVLGMDCGLNLKEKAKEI 426

[Close]

BLAST of CmaCh10G008610 vs. ExPASy Swiss-Prot
Match: Q9LT79 (U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2 SV=1)

HSP 1 Score: 145.6 bits (366), Expect = 1.2e-33
Identity = 115/391 (29.41%), Postives = 188/391 (48.08%), Query Frame = 0


Query: 4   IEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKK--CPVTKQPLIGIDLTPN 63
           I++P  F CPISL++M DPVT+ TG TYDR SIE W+       CPVT+ PL    L PN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 64  HTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILK-EAMRGR----SSRLECLNK 123
           HTLRR+IQ W   N  +GVER+ +PK   D   V  L+ +  A+ G      SR   L +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 124 LEAFLAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHL 183
           L  F  ++++N++ +    + E L  +L +    +E V    A+ ++L I  P   +   
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFVS-- 191

Query: 184 LIQNANLIDAVISIV--AVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRAL 243
           +  +   ++ +  ++  +  +T  +  A I  +S+    +D    I   +++F  +   L
Sbjct: 192 ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLDLL 251

Query: 244 NDQIAIKPALKI----LLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMD 303
            + I+ + ALKI    L  L      R  A+  G    L++ L       + E A+  ++
Sbjct: 252 RNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATVE 311

Query: 304 HLCECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVE 363
            LC   EG A    H   + ++ + ILRVS+ + E A   L  +C          EE   
Sbjct: 312 LLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC---TAEERWREEAAG 371

Query: 364 VGAVGKLCLMLQIGGSLKTNERIKEILHFLR 382
            G V +L LM+Q   + +  ++ +++L  LR
Sbjct: 372 AGVVVQLLLMVQSECTERAKKKAQKLLKLLR 397

[Close]

BLAST of CmaCh10G008610 vs. ExPASy Swiss-Prot
Match: Q9C8D1 (U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1 SV=1)

HSP 1 Score: 141.4 bits (355), Expect = 2.3e-32
Identity = 109/394 (27.66%), Postives = 196/394 (49.75%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWL-LFCKKCPVTKQPLIGIDLTPN 62
           EI +PS F CPIS ++M DPV I++GITYDRE+IEKW     + CPVT   L  ++  PN
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPN 89

Query: 63  HTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGR----SSRLECLNKL 122
           HT+RR+IQGW   +   G+ER+ +P+       V ++  + +   R    ++ +E + K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 123 EAFLAETERN-KIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHL 182
                E+ERN K   ++   +          E+ N  +++E+ + ++       T +  +
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASLLLEETVSVL-------TWMLPI 209

Query: 183 LIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTH-KIFTKDN-LFIQLSRAL 242
            ++  + +    S   + +  R+     AFL       + TH    TK N +     +++
Sbjct: 210 GLEGQSKLTTTSSFNRLVELLRNGDQNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 243 NDQIAIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRE---CELAIMAMDH 302
           N       +L  + H+    +   + V   +  DLV + +   V  E   CE A+  ++ 
Sbjct: 270 NRDSTCVNSLISIHHMILTNQ---ETVSRFLELDLVNITVEMLVDSENSVCEKALTVLNV 329

Query: 303 LCECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEV 362
           +CE  EGR ++ R+   + ++ +KIL++S    +  V +++ VCK+  G+    EE + +
Sbjct: 330 ICETKEGREKVRRNKLVIPILVKKILKIS--EKKDLVSVMWKVCKS--GDGSEVEEALRL 389

Query: 363 GAVGKLCLMLQIGGSLKTNERIKEILHFLRCIFK 386
           GA  KL +MLQ+G    T E++ E+L  +  + K
Sbjct: 390 GAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMK 409

[Close]

BLAST of CmaCh10G008610 vs. ExPASy TrEMBL
Match: A0A6J1JH48 (RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485066 PE=4 SV=1)

HSP 1 Score: 784.3 bits (2024), Expect = 2.5e-223
Identity = 400/400 (100.00%), Postives = 400/400 (100.00%), Query Frame = 0


Query: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP 60
           MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP
Sbjct: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP 60

Query: 61  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 120
           NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF
Sbjct: 61  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 120

Query: 121 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN 180
           LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN
Sbjct: 121 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN 180

Query: 181 ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI 240
           ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI
Sbjct: 181 ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI 240

Query: 241 KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA 300
           KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA
Sbjct: 241 KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA 300

Query: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360
           ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM
Sbjct: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360

Query: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF
Sbjct: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

[Close]

BLAST of CmaCh10G008610 vs. ExPASy TrEMBL
Match: A0A6J1HD28 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461732 PE=4 SV=1)

HSP 1 Score: 748.8 bits (1932), Expect = 1.2e-212
Identity = 381/400 (95.25%), Postives = 386/400 (96.50%), Query Frame = 0


Query: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP 60
           M EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK CPVTKQPLIG DLTP
Sbjct: 1   MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKNCPVTKQPLIGTDLTP 60

Query: 61  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 120
           NHTLRRVIQGW CVN EHG ERVRSPKGEGDGGHVVK+ILKEAMRGRSSRLECLNKLEAF
Sbjct: 61  NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 120

Query: 121 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN 180
           LAETERNKIYLQDARSMEFL SMLNNPED NE+VIVEKAIEII NINSPTTLLEHLLI+N
Sbjct: 121 LAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEHLLIRN 180

Query: 181 ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI 240
           +NLID VISIVAVTKTSRSR AAIAFLSSLYSISDQTHK FTKD+LFIQLSRALNDQIAI
Sbjct: 181 SNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALNDQIAI 240

Query: 241 KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA 300
           KP LKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMD LCECAEGRA
Sbjct: 241 KPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCECAEGRA 300

Query: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360
           ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM
Sbjct: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360

Query: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF
Sbjct: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

[Close]

BLAST of CmaCh10G008610 vs. ExPASy TrEMBL
Match: A0A0A0LXW1 (RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G635930 PE=4 SV=1)

HSP 1 Score: 571.6 bits (1472), Expect = 2.5e-159
Identity = 296/407 (72.73%), Postives = 341/407 (83.78%), Query Frame = 0


Query: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK----KCPVTKQPLIGI 60
           MAEIEVPSDFLCPISLQ+M DPVTISTGITYDRESI+KWL  CK     CPV+KQ L  +
Sbjct: 1   MAEIEVPSDFLCPISLQLMRDPVTISTGITYDRESIKKWLSSCKNQPLSCPVSKQALSSV 60

Query: 61  DLTPNHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRG-RSSRLECLN 120
           DLTPNHTLRRVIQGW  +N  HGVE++ +P+ E D G VVKLILKEAM+  RSS LECL 
Sbjct: 61  DLTPNHTLRRVIQGWCSLNERHGVEQIPTPEDESDRGDVVKLILKEAMKSPRSSMLECLK 120

Query: 121 KLEAFLAETERNKIYLQDARSMEFLTSML--NNPEDENEEVIVEKAIEIILNINSPTTLL 180
           +L++ +AE E NKIYLQ+  SM FLTS+L  +  EDE+E+ +VE+AIEII +INSPT +L
Sbjct: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEDEDEDEDAMVEEAIEIITDINSPTIIL 180

Query: 181 EHLLIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRA 240
            ++L QN  LID +ISI+  TK+ +SR + IAFLSSLY+ISDQ HK FTKD LFIQL+RA
Sbjct: 181 NNVLTQNPQLIDTLISIITSTKSPKSRASTIAFLSSLYTISDQMHKTFTKDILFIQLTRA 240

Query: 241 LNDQIAIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLC 300
           L DQ+A KPAL+ILL LAPFGRNRIKAVKHGVVF +VELLLNST G+ECELA+  +D LC
Sbjct: 241 LKDQVATKPALQILLRLAPFGRNRIKAVKHGVVFCVVELLLNSTNGKECELAMAVLDRLC 300

Query: 301 ECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGA 360
           ECAEGRAELL+HGGGMAVV RKILRVSN  NEKAVRILY+VCKNNVGN GVAEEMVEVG 
Sbjct: 301 ECAEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGV 360

Query: 361 VGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           VGKLCLMLQ+GG+LKT ERIKEILH L+C+FKGTKCV V+PSGFDRF
Sbjct: 361 VGKLCLMLQVGGNLKTKERIKEILHLLQCVFKGTKCV-VVPSGFDRF 406

[Close]

BLAST of CmaCh10G008610 vs. ExPASy TrEMBL
Match: A0A1S3BNV9 (RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491935 PE=4 SV=1)

HSP 1 Score: 559.7 bits (1441), Expect = 1.0e-155
Identity = 290/405 (71.60%), Postives = 336/405 (82.96%), Query Frame = 0


Query: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKK----CPVTKQPLIGI 60
           MAEIEVPSDFLCPISLQIM DPVTISTGITYDRE I+KWL  CK     CPV+KQ L  +
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRECIKKWLSSCKNQPLICPVSKQALSSV 60

Query: 61  DLTPNHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRG-RSSRLECLN 120
           DLTPNHTL RVIQGW  ++  HGVE++ +PK E D G  VK ILKEAM+  RSSRLECL 
Sbjct: 61  DLTPNHTLGRVIQGWCSLHERHGVEQIPTPKDEIDRGDAVKSILKEAMKSPRSSRLECLK 120

Query: 121 KLEAFLAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEH 180
           +L++ +AE E NKIYLQ+  SM FLTS+L   E E+E+V+VE+AIEII NINSP T+L++
Sbjct: 121 RLKSIVAENESNKIYLQNVESMVFLTSILFEDEGEDEDVMVEEAIEIITNINSPATILKN 180

Query: 181 LLIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALN 240
           LL QN  LID +ISI+  TK+ +SR + IAFL SLY+ISDQTHK FTKD LFIQL+RAL 
Sbjct: 181 LLTQNPQLIDTLISIITSTKSPKSRASTIAFLCSLYTISDQTHKTFTKDILFIQLTRALM 240

Query: 241 DQIAIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCEC 300
           DQI+ KPAL+ILL LAPFGRNRIKA+K+GVVF +VELLLNS+ GRECELA+  +D LCEC
Sbjct: 241 DQISTKPALQILLRLAPFGRNRIKAIKNGVVFHVVELLLNSSNGRECELAMAVLDRLCEC 300

Query: 301 AEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVG 360
           AEGRAELL+HGGGMAVV RKILRVSN  NEKAVRILY+VCKNNVGN GVAEEMVEVG V 
Sbjct: 301 AEGRAELLQHGGGMAVVGRKILRVSNLGNEKAVRILYNVCKNNVGNCGVAEEMVEVGVVA 360

Query: 361 KLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           KLCLMLQ+GG+LKT ER+KEILH L+C+ +GTKCV V+PSGFDRF
Sbjct: 361 KLCLMLQVGGNLKTRERVKEILHLLQCVSRGTKCV-VVPSGFDRF 404

[Close]

BLAST of CmaCh10G008610 vs. ExPASy TrEMBL
Match: A0A6J1EH89 (RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434282 PE=4 SV=1)

HSP 1 Score: 529.3 bits (1362), Expect = 1.4e-146
Identity = 276/403 (68.49%), Postives = 323/403 (80.15%), Query Frame = 0


Query: 4   IEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK-------KCPVTKQPLIGI 63
           +EVPSDFLCPISLQIM DPVTISTGITYDRESIEKWL   K        CPVTK PL  +
Sbjct: 3   VEVPSDFLCPISLQIMRDPVTISTGITYDRESIEKWLSSYKNRHVVVPSCPVTKLPLSEL 62

Query: 64  DLTPNHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNK 123
           DLTPNHTLRR+IQGW  +N   GVE + +PKGE D G VVKL+ KEAMR  SSRLECL +
Sbjct: 63  DLTPNHTLRRLIQGWCSLNAGDGVEPIPTPKGEVDRGQVVKLV-KEAMRCGSSRLECLKR 122

Query: 124 LEAFLAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHL 183
           L+A + E ERNK YLQ+  SMEFLTS+LNN    N+E ++E+AIEI+ N NSPTTLL+HL
Sbjct: 123 LKAIVVENERNKFYLQEVESMEFLTSVLNN----NDESMIEEAIEILSNTNSPTTLLKHL 182

Query: 184 LIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALND 243
           L QN NLI  +ISI+ +TK+S S  AAIAFLSSLY+ISDQTHK FT+D LFI L+RAL+D
Sbjct: 183 LTQNPNLIQTLISIITITKSSISSAAAIAFLSSLYTISDQTHKTFTQDTLFIHLTRALSD 242

Query: 244 QIAIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECA 303
           QIA  P+L+ILLHLAP GRNR KAVKHG VF L++LL  S++ RECELA+  +D LCECA
Sbjct: 243 QIATNPSLQILLHLAPHGRNRFKAVKHGAVFHLIQLLFGSSIPRECELAMATLDRLCECA 302

Query: 304 EGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGK 363
           EGR ELLRHGGG+AVVARKILRVSN +NEKAVRILY+VCKNNVGNF V EEMVEVGAVGK
Sbjct: 303 EGRVELLRHGGGVAVVARKILRVSNLTNEKAVRILYNVCKNNVGNFRVFEEMVEVGAVGK 362

Query: 364 LCLMLQIGGSLKTNERIKEILHFLRCIFKG-TKCVLVLPSGFD 399
           LC ++++GGSLK  ER+K ILH ++  F G +KCV+V+P  FD
Sbjct: 363 LCAVIEVGGSLKIIERVKVILHLIQRAFDGSSKCVIVIPPRFD 400

[Close]

BLAST of CmaCh10G008610 vs. NCBI nr
Match: XP_022987530.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima])

HSP 1 Score: 784.3 bits (2024), Expect = 5.1e-223
Identity = 400/400 (100.00%), Postives = 400/400 (100.00%), Query Frame = 0


Query: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP 60
           MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP
Sbjct: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP 60

Query: 61  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 120
           NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF
Sbjct: 61  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 120

Query: 121 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN 180
           LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN
Sbjct: 121 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN 180

Query: 181 ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI 240
           ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI
Sbjct: 181 ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI 240

Query: 241 KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA 300
           KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA
Sbjct: 241 KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA 300

Query: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360
           ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM
Sbjct: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360

Query: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF
Sbjct: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

[Close]

BLAST of CmaCh10G008610 vs. NCBI nr
Match: XP_023516896.1 (E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo])

HSP 1 Score: 755.0 bits (1948), Expect = 3.3e-214
Identity = 386/400 (96.50%), Postives = 388/400 (97.00%), Query Frame = 0


Query: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP 60
           MAEIEVPSDFLCPISLQIMSDPVTISTGITY+RESIEKWLLFCK C VTKQPLIG DLTP
Sbjct: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYNRESIEKWLLFCKNCLVTKQPLIGTDLTP 60

Query: 61  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 120
           NHTLRRVIQGW CVN EHG ERVRSPKGEGDGGHVVK+ILKEAMRGRSSRLECLNKLEAF
Sbjct: 61  NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 120

Query: 121 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN 180
           LAETERNKIYLQDARSMEFL SMLNNPEDENEEVIVEKAIEII NINSPTTLLEHLLIQN
Sbjct: 121 LAETERNKIYLQDARSMEFLASMLNNPEDENEEVIVEKAIEIISNINSPTTLLEHLLIQN 180

Query: 181 ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI 240
           ANLIDAVISIVAVTKTSRSR AAIAFLSSLYSISDQTHK FTKDNLFIQLSRALNDQIAI
Sbjct: 181 ANLIDAVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDNLFIQLSRALNDQIAI 240

Query: 241 KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA 300
           KP LKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMD LCECAEGRA
Sbjct: 241 KPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCECAEGRA 300

Query: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360
           ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM
Sbjct: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360

Query: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF
Sbjct: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

[Close]

BLAST of CmaCh10G008610 vs. NCBI nr
Match: KAG6590132.1 (E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 754.2 bits (1946), Expect = 5.7e-214
Identity = 383/400 (95.75%), Postives = 388/400 (97.00%), Query Frame = 0


Query: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP 60
           MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK CPVTKQPLIG DLTP
Sbjct: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKNCPVTKQPLIGTDLTP 60

Query: 61  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 120
           NHTLRRVIQGWSCVN EHG ERVRSPKGEGDGGHVVK+ILKEAMRGRSSRLECLNKLEAF
Sbjct: 61  NHTLRRVIQGWSCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 120

Query: 121 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN 180
           LAETERNKIYLQDARSMEFL SMLNNPED NEEV+VEKAIEII NINSPTTLLEHLLIQN
Sbjct: 121 LAETERNKIYLQDARSMEFLASMLNNPEDGNEEVLVEKAIEIISNINSPTTLLEHLLIQN 180

Query: 181 ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI 240
           +NLID VISI+AVTKTSRSR AAIAFLSSLYSISDQTHK FTKD+LFIQLSRALNDQIAI
Sbjct: 181 SNLIDVVISIIAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALNDQIAI 240

Query: 241 KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA 300
           KP LKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMD LCECAEGRA
Sbjct: 241 KPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCECAEGRA 300

Query: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360
           ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM
Sbjct: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360

Query: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF
Sbjct: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

[Close]

BLAST of CmaCh10G008610 vs. NCBI nr
Match: XP_022961129.1 (E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata])

HSP 1 Score: 748.8 bits (1932), Expect = 2.4e-212
Identity = 381/400 (95.25%), Postives = 386/400 (96.50%), Query Frame = 0


Query: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPLIGIDLTP 60
           M EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK CPVTKQPLIG DLTP
Sbjct: 1   MPEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKNCPVTKQPLIGTDLTP 60

Query: 61  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 120
           NHTLRRVIQGW CVN EHG ERVRSPKGEGDGGHVVK+ILKEAMRGRSSRLECLNKLEAF
Sbjct: 61  NHTLRRVIQGWCCVNAEHGAERVRSPKGEGDGGHVVKVILKEAMRGRSSRLECLNKLEAF 120

Query: 121 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLLIQN 180
           LAETERNKIYLQDARSMEFL SMLNNPED NE+VIVEKAIEII NINSPTTLLEHLLI+N
Sbjct: 121 LAETERNKIYLQDARSMEFLASMLNNPEDGNEDVIVEKAIEIISNINSPTTLLEHLLIRN 180

Query: 181 ANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQIAI 240
           +NLID VISIVAVTKTSRSR AAIAFLSSLYSISDQTHK FTKD+LFIQLSRALNDQIAI
Sbjct: 181 SNLIDVVISIVAVTKTSRSRAAAIAFLSSLYSISDQTHKTFTKDDLFIQLSRALNDQIAI 240

Query: 241 KPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAEGRA 300
           KP LKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMD LCECAEGRA
Sbjct: 241 KPTLKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDQLCECAEGRA 300

Query: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360
           ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM
Sbjct: 301 ELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLM 360

Query: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF
Sbjct: 361 LQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 400

[Close]

BLAST of CmaCh10G008610 vs. NCBI nr
Match: XP_038879749.1 (E3 ubiquitin-protein ligase PUB23-like isoform X1 [Benincasa hispida])

HSP 1 Score: 590.5 bits (1521), Expect = 1.1e-164
Identity = 303/403 (75.19%), Postives = 341/403 (84.62%), Query Frame = 0


Query: 1   MAEIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCK--KCPVTKQPLIGIDL 60
           MAEIEVPSDFLCPISLQIM DPVTISTGITYDRESIEKWL  CK   CPVTKQ L GIDL
Sbjct: 1   MAEIEVPSDFLCPISLQIMRDPVTISTGITYDRESIEKWLSSCKNLSCPVTKQALGGIDL 60

Query: 61  TPNHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRG-RSSRLECLNKL 120
           TPNHTLRRVIQGW  +N  HGVE++ +P  E D G VVKLILKEA++G RSSRLECL +L
Sbjct: 61  TPNHTLRRVIQGWCSLNNRHGVEQIPTPNSEDDRGDVVKLILKEAVKGPRSSRLECLKRL 120

Query: 121 EAFLAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLL 180
           +  +AE+ERNKIY Q+  SME L S+L   EDE+E  IVE+ IEII NINS T LL+H+L
Sbjct: 121 KDIVAESERNKIYFQNVESMECLASILK--EDEDEAAIVEETIEIISNINSSTALLKHIL 180

Query: 181 IQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQ 240
            QN  LID +ISI+ +TKTS+SR A IAFLSSLY+ISDQTHK FTK+ +F+QL+RAL DQ
Sbjct: 181 TQNPRLIDTLISIITITKTSKSRAATIAFLSSLYTISDQTHKTFTKEIIFVQLTRALKDQ 240

Query: 241 IAIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMDHLCECAE 300
           IA KP LKILLHLAPFGRNRIKAVK+G VF +VELLL+S+ GRECELA+  +D LCECAE
Sbjct: 241 IATKPTLKILLHLAPFGRNRIKAVKNGAVFHVVELLLHSSNGRECELAMAVLDRLCECAE 300

Query: 301 GRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKL 360
           GRAELLRHGGGMAVV RKILRVSN  NEKAVRI+Y+VCKNNVGN GV EEMVEVG VGKL
Sbjct: 301 GRAELLRHGGGMAVVGRKILRVSNLGNEKAVRIVYNVCKNNVGNLGVVEEMVEVGVVGKL 360

Query: 361 CLMLQIGGSLKTNERIKEILHFLRCIFKGTKCVLVLPSGFDRF 401
           CLMLQ+GG+LKT ERIKEILH L+C+FKGTKCVLV+PSGFDRF
Sbjct: 361 CLMLQVGGNLKTKERIKEILHLLQCVFKGTKCVLVIPSGFDRF 401

[Close]

BLAST of CmaCh10G008610 vs. TAIR 10
Match: AT3G52450.1 (plant U-box 22 )

HSP 1 Score: 271.6 bits (693), Expect = 1.0e-72
Identity = 166/429 (38.69%), Postives = 251/429 (58.51%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKK--CPVTKQPLIGIDLTP 62
           EIE+PS FLCPISL IM DPV +STGITYDRESIEKWL   KK  CPVTKQ +   DLTP
Sbjct: 4   EIEIPSFFLCPISLDIMKDPVIVSTGITYDRESIEKWLFSGKKNSCPVTKQVITETDLTP 63

Query: 63  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 122
           NHTLRR+IQ W  +N  +G+ER+ +PK       + KLI KE+     ++++CL +L   
Sbjct: 64  NHTLRRLIQSWCTLNASYGIERIPTPKPPICKSEIEKLI-KESSSSHLNQVKCLKRLRQI 123

Query: 123 LAETERNKIYLQDARSMEFLTSMLNNPED---------------------------ENEE 182
           ++E   NK  L+ A   EFL ++++N  D                           ++  
Sbjct: 124 VSENTTNKRCLEAAEVPEFLANIVSNSVDTYNSPSSSLSSSNLNDMCQSNMLENRFDSSR 183

Query: 183 VIVEKAIEIILNINSPTTLLEHLL--IQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLY 242
            ++++A+ ++ ++++  T L+ LL   +  NL+  +  I+       SR  A   L  L 
Sbjct: 184 SLMDEALSVLYHLDTSETALKSLLNNKKGTNLVKTLTKIMQ-RGIYESRAYAALLLKKLL 243

Query: 243 SISDQTHKIFTKDNLFIQLSRALNDQI---AIKPALKILLHLAPFGRNRIKAVKHGVVFD 302
            ++D    I  +  LF ++ + L+DQI   A + A++IL+   P+GRNR KAV+ G +  
Sbjct: 244 EVADPMQIILLERELFGEVIQILHDQISHKATRSAMQILVITCPWGRNRHKAVEGGTISM 303

Query: 303 LVELLLNSTVG---RECELAIMAMDHLCECAEGRAELLRHGGGMAVVARKILRVSNFSNE 362
           ++ELL++ T     R  E+A++ +D LC+CAEGRAE L HG  +AVV++KILRVS  ++E
Sbjct: 304 IIELLMDDTFSSERRNSEMAMVVLDMLCQCAEGRAEFLNHGAAIAVVSKKILRVSQITSE 363

Query: 363 KAVRILYHVCKNNVGNF----GVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEILHFLR 391
           +AVR+L      +VG F     + +EM+++G V KLCL+LQ+    KT E+ KE+L    
Sbjct: 364 RAVRVLL-----SVGRFCATPSLLQEMLQLGVVAKLCLVLQVSCGNKTKEKAKELLKLHA 423

[Close]

BLAST of CmaCh10G008610 vs. TAIR 10
Match: AT2G35930.1 (plant U-box 23 )

HSP 1 Score: 271.2 bits (692), Expect = 1.4e-72
Identity = 158/402 (39.30%), Postives = 252/402 (62.69%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKK--CPVTKQPLIGIDLTP 62
           EIE+P  FLCPISL+IM DPV +STGITYDR+SIEKWL   KK  CPVTKQ +   DLTP
Sbjct: 9   EIEIPPFFLCPISLEIMKDPVIVSTGITYDRDSIEKWLFAGKKNSCPVTKQDITDADLTP 68

Query: 63  NHTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGRSSRLECLNKLEAF 122
           NHTLRR+IQ W  +N  +GVER+ +P+       + KLI +++     ++++CL +L   
Sbjct: 69  NHTLRRLIQSWCTLNASYGVERIPTPRPPICKSEIEKLI-RDSASSHENQVKCLKRLRQI 128

Query: 123 LAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHLL--I 182
           ++E   NK  L+ A   EFL ++++N  D     + ++A+ ++ ++ +  T+L++LL   
Sbjct: 129 VSENATNKRCLEAAGVPEFLANIVSN--DSENGSLTDEALNLLYHLETSETVLKNLLNNK 188

Query: 183 QNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRALNDQI 242
           ++ N++ ++  I+       SR  A   L ++  ++D    +  K  +F ++ + L+D+I
Sbjct: 189 KDNNIVKSLTKIMQ-RGMYESRVYATLLLKNILEVADPMQSMTLKPEVFTEVVQILDDRI 248

Query: 243 ---AIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRE---CELAIMAMDHL 302
              A K A+ IL+++ P+GRNR KAV+ GV+  ++ELL++ +   E    E+A++ +D L
Sbjct: 249 SQKATKAAMHILVNICPWGRNRHKAVEAGVISVIIELLMDESFTSERRGPEMAMVVLDLL 308

Query: 303 CECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNF----GVAEEM 362
           C+CAEGRAE L HG  +AVV +KILRVS  ++++AVR+L      +VG F     +  EM
Sbjct: 309 CQCAEGRAEFLNHGAAIAVVCKKILRVSQTASDRAVRVLL-----SVGRFCATPALLHEM 368

Query: 363 VEVGAVGKLCLMLQIGGSLKTNERIKEILHFLRCIFKGTKCV 391
           +++G V KLCL+LQ+    KT E+ KE+L     ++K + C+
Sbjct: 369 LQLGVVAKLCLVLQVSCGGKTKEKAKELLKLHARVWKDSPCL 401

[Close]

BLAST of CmaCh10G008610 vs. TAIR 10
Match: AT3G11840.1 (plant U-box 24 )

HSP 1 Score: 184.9 bits (468), Expect = 1.3e-46
Identity = 145/425 (34.12%), Postives = 220/425 (51.76%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKKCPVTKQPL-IGIDLTPN 62
           EIE+P+ F+CPISL+IM DPVT  +GITYDR++I KWL     CPVTKQPL +  DLTPN
Sbjct: 21  EIEIPNYFICPISLEIMKDPVTTVSGITYDRQNIVKWLEKVPSCPVTKQPLPLDSDLTPN 80

Query: 63  HTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLI--LKEAMRGRSSRLECLNKLEA 122
           H LRR+IQ W   N   GV R+ +P+      +VV+ I  LK+  +    R E L KLE 
Sbjct: 81  HMLRRLIQHWCVENETRGVVRISTPRVPPGKLNVVEEIKNLKKFGQEALGREETLQKLEV 140

Query: 123 FLAETERNKIYLQ---DARSMEFLTSMLNNPEDENEEVI-VEKAIEIILNINSPTTLLEH 182
              +    ++  +       + F+    +  ED    +  +++++ ++  I  P+   + 
Sbjct: 141 LAMDGNNRRLMCECGVHKSLILFVVKCTSEDEDGRRRIKGLDESLRLLHLIGIPSNDAKT 200

Query: 183 LLIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSL--YSISDQTHKI----------FTK 242
           +L++N  ++++ ++ V   +   S+   I  L +L  Y+ S    ++          F K
Sbjct: 201 ILMENDRVMES-LTWVLHQEDFLSKAYTIVLLRNLTEYTSSHIVERLNPEIFKGIIGFLK 260

Query: 243 D--NLFIQLSRALNDQI------------------------AIKPALKILLHLAPFGRNR 302
           D  N   + S  + + +                        A+  AL ILL  + + RNR
Sbjct: 261 DVVNSVNRTSPTVRETVQSSSRPSLGKTEPSKLDHSLVIKQAVTAALMILLETSSWSRNR 320

Query: 303 IKAVKHGVVFDLVELLLNST-VGRECELAIMAMDHLCECAEGRAELLRHGGGMAVVARKI 362
              V  G V +L+EL ++ T   R  EL +  +  LC CA GRAE+L H GG+AVV +++
Sbjct: 321 SLLVDLGAVSELIELEISYTGEKRITELMLGVLSRLCCCANGRAEILAHRGGIAVVTKRL 380

Query: 363 LRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEVGAVGKLCLMLQIGGSLKTNERIKEI 380
           LRVS  ++++A+ IL  V K +  N  V EEMV VG V KLC +L +   L   E+ KEI
Sbjct: 381 LRVSPAADDRAISILTTVSKFSPENM-VVEEMVNVGTVEKLCSVLGMDCGLNLKEKAKEI 440

[Close]

BLAST of CmaCh10G008610 vs. TAIR 10
Match: AT3G19380.1 (plant U-box 25 )

HSP 1 Score: 145.6 bits (366), Expect = 8.6e-35
Identity = 115/391 (29.41%), Postives = 188/391 (48.08%), Query Frame = 0


Query: 4   IEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWLLFCKK--CPVTKQPLIGIDLTPN 63
           I++P  F CPISL++M DPVT+ TG TYDR SIE W+       CPVT+ PL    L PN
Sbjct: 12  IQIPYHFRCPISLELMQDPVTVCTGQTYDRASIESWVSIGNNTTCPVTRAPLSDFTLIPN 71

Query: 64  HTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILK-EAMRGR----SSRLECLNK 123
           HTLRR+IQ W   N  +GVER+ +PK   D   V  L+ +  A+ G      SR   L +
Sbjct: 72  HTLRRLIQEWCVANRSNGVERIPTPKQPADPTSVRALLSQASAITGTHVSVRSRAAALRR 131

Query: 124 LEAFLAETERNKIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHL 183
           L  F  ++++N++ +    + E L  +L +    +E V    A+ ++L I  P   +   
Sbjct: 132 LRGFARDSDKNRVLIAAHNATEILIKILFSETTSSELVSESLALLVMLPITEPNQFVS-- 191

Query: 184 LIQNANLIDAVISIV--AVTKTSRSRGAAIAFLSSLYSISDQTHKIFTKDNLFIQLSRAL 243
           +  +   ++ +  ++  +  +T  +  A I  +S+    +D    I   +++F  +   L
Sbjct: 192 ISSDPGRVEFLTRLLFDSSIETRVNAAALIEIVSTGTKSADLKGSISNSESVFEGVLDLL 251

Query: 244 NDQIAIKPALKI----LLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRECELAIMAMD 303
            + I+ + ALKI    L  L      R  A+  G    L++ L       + E A+  ++
Sbjct: 252 RNPISSRRALKIGIKTLFALCSVKSTRHIAITAGAPEILIDRLAADFDRCDTERALATVE 311

Query: 304 HLCECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVE 363
            LC   EG A    H   + ++ + ILRVS+ + E A   L  +C          EE   
Sbjct: 312 LLCRTPEGCAAFGEHALTVPLLVKTILRVSDRATEYAAGALLALC---TAEERWREEAAG 371

Query: 364 VGAVGKLCLMLQIGGSLKTNERIKEILHFLR 382
            G V +L LM+Q   + +  ++ +++L  LR
Sbjct: 372 AGVVVQLLLMVQSECTERAKKKAQKLLKLLR 397

[Close]

BLAST of CmaCh10G008610 vs. TAIR 10
Match: AT1G66160.1 (CYS, MET, PRO, and GLY protein 1 )

HSP 1 Score: 141.4 bits (355), Expect = 1.6e-33
Identity = 109/394 (27.66%), Postives = 196/394 (49.75%), Query Frame = 0


Query: 3   EIEVPSDFLCPISLQIMSDPVTISTGITYDRESIEKWL-LFCKKCPVTKQPLIGIDLTPN 62
           EI +PS F CPIS ++M DPV I++GITYDRE+IEKW     + CPVT   L  ++  PN
Sbjct: 30  EITIPSQFQCPISYELMKDPVIIASGITYDRENIEKWFESGYQTCPVTNTVLTSLEQIPN 89

Query: 63  HTLRRVIQGWSCVNTEHGVERVRSPKGEGDGGHVVKLILKEAMRGR----SSRLECLNKL 122
           HT+RR+IQGW   +   G+ER+ +P+       V ++  + +   R    ++ +E + K+
Sbjct: 90  HTIRRMIQGWCGSSLGGGIERIPTPRVPVTSHQVSEICERLSAATRRGDYAACMEMVTKM 149

Query: 123 EAFLAETERN-KIYLQDARSMEFLTSMLNNPEDENEEVIVEKAIEIILNINSPTTLLEHL 182
                E+ERN K   ++   +          E+ N  +++E+ + ++       T +  +
Sbjct: 150 TRLGKESERNRKCVKENGAGLVLCVCFDAFSENANASLLLEETVSVL-------TWMLPI 209

Query: 183 LIQNANLIDAVISIVAVTKTSRSRGAAIAFLSSLYSISDQTH-KIFTKDN-LFIQLSRAL 242
            ++  + +    S   + +  R+     AFL       + TH    TK N +     +++
Sbjct: 210 GLEGQSKLTTTSSFNRLVELLRNGDQNAAFLIKELLELNVTHVHALTKINGVQEAFMKSI 269

Query: 243 NDQIAIKPALKILLHLAPFGRNRIKAVKHGVVFDLVELLLNSTVGRE---CELAIMAMDH 302
           N       +L  + H+    +   + V   +  DLV + +   V  E   CE A+  ++ 
Sbjct: 270 NRDSTCVNSLISIHHMILTNQ---ETVSRFLELDLVNITVEMLVDSENSVCEKALTVLNV 329

Query: 303 LCECAEGRAELLRHGGGMAVVARKILRVSNFSNEKAVRILYHVCKNNVGNFGVAEEMVEV 362
           +CE  EGR ++ R+   + ++ +KIL++S    +  V +++ VCK+  G+    EE + +
Sbjct: 330 ICETKEGREKVRRNKLVIPILVKKILKIS--EKKDLVSVMWKVCKS--GDGSEVEEALRL 389

Query: 363 GAVGKLCLMLQIGGSLKTNERIKEILHFLRCIFK 386
           GA  KL +MLQ+G    T E++ E+L  +  + K
Sbjct: 390 GAFKKLVVMLQVGCGEGTKEKVTELLKMMNKVMK 409

The following BLAST results are available for this feature:

BLAST of CmaCh10G008610 vs. ExPASy Swiss-Prot


Match Name	E-value	Identity	Description
Q9SVC6	1.5e-71	38.69	E3 ubiquitin-protein ligase PUB22 OS=Arabidopsis thaliana OX=3702 GN=PUB22 PE=1 ...	[more]
Q84TG3	1.9e-71	39.30	E3 ubiquitin-protein ligase PUB23 OS=Arabidopsis thaliana OX=3702 GN=PUB23 PE=1 ...	[more]
Q9SF15	1.8e-45	34.12	E3 ubiquitin-protein ligase PUB24 OS=Arabidopsis thaliana OX=3702 GN=PUB24 PE=1 ...	[more]
Q9LT79	1.2e-33	29.41	U-box domain-containing protein 25 OS=Arabidopsis thaliana OX=3702 GN=PUB25 PE=2...	[more]
Q9C8D1	2.3e-32	27.66	U-box domain-containing protein 20 OS=Arabidopsis thaliana OX=3702 GN=PUB20 PE=1...	[more]

BLAST of CmaCh10G008610 vs. ExPASy TrEMBL


Match Name	E-value	Identity	Description
A0A6J1JH48	2.5e-223	100.00	RING-type E3 ubiquitin transferase OS=Cucurbita maxima OX=3661 GN=LOC111485066 P...	[more]
A0A6J1HD28	1.2e-212	95.25	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111461732...	[more]
A0A0A0LXW1	2.5e-159	72.73	RING-type E3 ubiquitin transferase OS=Cucumis sativus OX=3659 GN=Csa_1G635930 PE...	[more]
A0A1S3BNV9	1.0e-155	71.60	RING-type E3 ubiquitin transferase OS=Cucumis melo OX=3656 GN=LOC103491935 PE=4 ...	[more]
A0A6J1EH89	1.4e-146	68.49	RING-type E3 ubiquitin transferase OS=Cucurbita moschata OX=3662 GN=LOC111434282...	[more]

BLAST of CmaCh10G008610 vs. NCBI nr


Match Name	E-value	Identity	Description
XP_022987530.1	5.1e-223	100.00	E3 ubiquitin-protein ligase PUB23-like [Cucurbita maxima]	[more]
XP_023516896.1	3.3e-214	96.50	E3 ubiquitin-protein ligase PUB22-like [Cucurbita pepo subsp. pepo]	[more]
KAG6590132.1	5.7e-214	95.75	E3 ubiquitin-protein ligase PUB22, partial [Cucurbita argyrosperma subsp. sorori...	[more]
XP_022961129.1	2.4e-212	95.25	E3 ubiquitin-protein ligase PUB23-like [Cucurbita moschata]	[more]
XP_038879749.1	1.1e-164	75.19	E3 ubiquitin-protein ligase PUB23-like isoform X1 [Benincasa hispida]	[more]

BLAST of CmaCh10G008610 vs. TAIR 10


Match Name	E-value	Identity	Description
AT3G52450.1	1.0e-72	38.69	plant U-box 22	[more]
AT2G35930.1	1.4e-72	39.30	plant U-box 23	[more]
AT3G11840.1	1.3e-46	34.12	plant U-box 24	[more]
AT3G19380.1	8.6e-35	29.41	plant U-box 25	[more]
AT1G66160.1	1.6e-33	27.66	CYS, MET, PRO, and GLY protein 1	[more]

InterPro

Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25


IPR Term	IPR Description	Source	Source Term	Source Description	Alignment
IPR003613	U box domain	SMART	SM00504	Ubox_2	coord: 9..71 e-value: 8.5E-29 score: 111.7
IPR003613	U box domain	PFAM	PF04564	U-box	coord: 6..75 e-value: 2.9E-18 score: 65.8
IPR003613	U box domain	PROSITE	PS51698	U_BOX	coord: 5..78 score: 36.101067
IPR011989	Armadillo-like helical	GENE3D	1.25.10.10		coord: 99..391 e-value: 2.0E-12 score: 48.4
IPR013083	Zinc finger, RING/FYVE/PHD-type	GENE3D	3.30.40.10	Zinc/RING finger domain, C3HC4 (zinc finger)	coord: 1..84 e-value: 6.5E-27 score: 95.4
None	No IPR available	PANTHER	PTHR22849	WDSAM1 PROTEIN	coord: 5..392
None	No IPR available	PANTHER	PTHR22849:SF11	OS11G0573500 PROTEIN	coord: 5..392
None	No IPR available	CDD	cd16664	RING-Ubox_PUB	coord: 10..51 e-value: 6.33514E-21 score: 83.0519
None	No IPR available	SUPERFAMILY	57850	RING/U-box	coord: 4..75
IPR016024	Armadillo-type fold	SUPERFAMILY	48371	ARM repeat	coord: 111..362

Relationships

The following mRNA feature(s) are a part of this gene:


Feature Name	Unique Name	Type
CmaCh10G008610.1	CmaCh10G008610.1	mRNA

GO Annotation

GO Assignments

This gene is annotated with the following GO terms.

Category	Term Accession	Term Name
biological_process	GO:0016567	protein ubiquitination
molecular_function	GO:0004842	ubiquitin-protein transferase activity