CmaCh10G008100 (gene) Cucurbita maxima (Rimu) v1.1

Overview
NameCmaCh10G008100
Typegene
OrganismCucurbita maxima (Cucurbita maxima (Rimu) v1.1)
Descriptionpumilio homolog 12-like
LocationCma_Chr10: 3754545 .. 3757299 (+)
RNA-Seq ExpressionCmaCh10G008100
SyntenyCmaCh10G008100
Sequences
The following sequences are available for this feature:

Gene sequence (with intron)

Legend: exonCDSpolypeptide
Hold the cursor over a type above to highlight its positions in the sequence below.
ATGGGCAGTAAACTGAAGCTTGAACTTGAGAAGCTTTTCAGTGAGGATCTGATAGTTGCTTGTGACAATGAACATGCTGAAGAATCTGTTCATAAAGGAATGTCTTCAATGGTTTCAGATTCTTGTAATGGGTTGTCCTTGGATGAACTCCACAATAATGGGAGTTTGGAGAATGGAAAGGTCTTGATCGGTCGATTTGGAGACCGATCGTTGACATCTGCAGTCGAGAAACTAAGTTTAGGGGGGGAGGGGACAGGGAAGACTTGGATGAATCACCCCAATTTGATGGATGATCAATTTGAAAGCTATTTGAATAAACAATCACTCAAAACTGAACCATCAATGGTGAGTGATCCCCCTTTGAGACCTTCACATTGTGTGAACAATGGCTATTATGAGATTCGTATGCCGGGTCTTCCCCCTCAGATCTCGGTCGAAGCAAGACCTGTTTCTGATGTCCACAAGAATGGGGAGGCGGGTCAGGTTCCGCCATTGAAGACTCCTGCTGCTATGCCATTCACTCGTCATGAACAAATGTTGCACAGTGGGTTATCCCCAATTCATTTTATGCGCCCACAACAAATGAATCATGGTGAAATAGGTTCAAATTGTACCAAAGAACAGCAGCTCCTAAGCTGCAGGATGCAAGGGCAGCACCAATACCTGTATGATCTTCATAATCAGCAACGGGAATATTCGAATCTATTTGAATCTTGTGGTAAACCGTTTGGGTCGTTGCGGTTTCGGAGTCCGAATCAGGAATGTTTTATTGAGGTTCCCTTTTCTCCCTATCTTGGGCAGTTTAAACATGAAGGATTCTGCAATAGGTCTGCACATTATGCAGGTTCGGGCGTACCAAATCTTGTATTTACAAAACCGAACATAGATACGTTAGATTCCCAGGAAAAAAGTTTCAATCAAAGCTCTCCTAGGAAGGTCCCCATGAGGACACATGGACCGATTGGAGTTGATTGTATGAAGGCTCCGATCGGAGTTGATGCTGAGAAACTTAAGTATTATATCAGCCAAAATGGATTTGGTTGTCCAAGTTGTATTGTTAGGCAGAATGGTTTACCTTCAACAACCAAAGATTGCATCTGTCATGACAACTTAAGGGCATCTTCTACGTTATCGAGTTACGAAAATCATCCGATAGAGATACTGCCTTCAAAATGTAACTCTCTTGACGAAGCCACAGGTAAAATATATCTCATGGCTAAAGATCAACATGGTTGTCGCTTCTTACAACGGATGTTCACTGACGGTACTAAAGAAGATATCGAGATGATTTTCGGTGAGATCATTCATCATGTTTCTGAGCTCATGATAGACCCTTTTGGAAACTATTTGATACAGAAACTCCTTGGAGTTTGTGACGAGGATCAACGCCTTCAAATTCTTTATAAGATTAACCGACCCGGTGAACTGATTCGAATCTCGTGTAATATGCATGGGTATGCTCTATCTCTGTCTGTCTTTGATCGAGTTCTCTTCTTTAATATTCATGTTTCCAAACTACAGGACTCGTGCCGTTCAGAAGCTCATCGAGACTCTCAAAACGCAAGAGCAGTTTTCCATGGTTGTCTCTTTATTGAAGACTGGTATAGTGATACTCATGAAGAATGTTAATGGTAACCATGTAGCACAGCATTGCCTGCAGTATTTGATGCCTGATTATATTGGTGTAAGATTTGTTTCTTGAAACTTTGCTCTCAATTTAGCTTGTCATCTTGCAATAAAATGGATTCTTCTGCAGTTTCTTTTTGATGCTGCAATAAAATCTTGTGTCGAGGTTGCAACTGATCGCCACGGTTGTTGCGTGCTTCAAAAGTGCCTTTCTGTTTCTGATGCTCGATACCGAGACCGTTTGTTAAGCGAGGTCGTACGTAATGCTTTGGTCCTTTCCCAAGATCAGTACGGGTAATTTATTTTTGTTCTCTGCACTCTACATTGTCGATAAGTGCTCGACTTTACGTTGCAATGATGCAGGAACTATGTCGTGCAATTCTCCCTTGAGCTTGGGCGCTGTCCTACGAGCATTCCTTGGGTGACGCCTGGTATCTTCAAACGACTAGAGGGCCATTTTGCAGACTTGTCTATCCAGAAATATAGCAGTAATGTTGTTGAAAAATGTGCTTACACTGGAGAAGATTATCTCTTTAAGATTATCGACGAGTTGATAAACGACGAACGATTCAGTCAAATTATGCTGAACCCATATGGAAACTATGCAGTTCAAGCTGTTCTTGCTCGTTCTTGGAATTTCAAAGTGAGTATTACTCCCCCTTTGACTCCTATGAAGTTAGTACTCACTGGTTGTTGAACTGTTTGATTTATACAATCTCTACTGAATTGAATTATGAACAGAGTCCTGTTCATGCTAAACTTGTGGCTGCTATCAGACCGCACGTCCCGTTGCTCAGGACGAACATGTATGGGAAGAAAGTCCTCGCCGTGCTAACAAAAGCGAGCTAAGTGGCCGCTATCATCGACGAAGATTGCCTGCCTTCTAACCTGCATTTCTTGTTGTTTGTTAAAAGGACATGACTAGTTTTGTCTCTCTTTTTTCTTTTGTTTGTTAAGGGAGCTTAGGCCATTAACCATGGCTGCAACTGTGCAACATGGTTCGTCTCCATATCTCGAAAGAAAACCAATCGGAGTTGAAGACGATCATCCATCACAATCCGTTCCAACAAATGGTAACATGTCTGTATTTTCCTTTGTAGATTCGCTGTTGTATTGGCGACCGAGATGA

mRNA sequence

ATGGGCAGTAAACTGAAGCTTGAACTTGAGAAGCTTTTCAGTGAGGATCTGATAGTTGCTTGTGACAATGAACATGCTGAAGAATCTGTTCATAAAGGAATGTCTTCAATGGTTTCAGATTCTTGTAATGGGTTGTCCTTGGATGAACTCCACAATAATGGGAGTTTGGAGAATGGAAAGGTCTTGATCGGTCGATTTGGAGACCGATCGTTGACATCTGCAGTCGAGAAACTAAGTTTAGGGGGGGAGGGGACAGGGAAGACTTGGATGAATCACCCCAATTTGATGGATGATCAATTTGAAAGCTATTTGAATAAACAATCACTCAAAACTGAACCATCAATGGTGAGTGATCCCCCTTTGAGACCTTCACATTGTGTGAACAATGGCTATTATGAGATTCGTATGCCGGGTCTTCCCCCTCAGATCTCGGTCGAAGCAAGACCTGTTTCTGATGTCCACAAGAATGGGGAGGCGGGTCAGGTTCCGCCATTGAAGACTCCTGCTGCTATGCCATTCACTCGTCATGAACAAATGTTGCACAGTGGGTTATCCCCAATTCATTTTATGCGCCCACAACAAATGAATCATGGTGAAATAGGTTCAAATTGTACCAAAGAACAGCAGCTCCTAAGCTGCAGGATGCAAGGGCAGCACCAATACCTGTATGATCTTCATAATCAGCAACGGGAATATTCGAATCTATTTGAATCTTGTGGTAAACCGTTTGGGTCGTTGCGGTTTCGGAGTCCGAATCAGGAATGTTTTATTGAGGTTCCCTTTTCTCCCTATCTTGGGCAGTTTAAACATGAAGGATTCTGCAATAGGTCTGCACATTATGCAGGTTCGGGCGTACCAAATCTTGTATTTACAAAACCGAACATAGATACGTTAGATTCCCAGGAAAAAAGTTTCAATCAAAGCTCTCCTAGGAAGGTCCCCATGAGGACACATGGACCGATTGGAGTTGATTGTATGAAGGCTCCGATCGGAGTTGATGCTGAGAAACTTAAGTATTATATCAGCCAAAATGGATTTGGTTGTCCAAGTTGTATTGTTAGGCAGAATGGTTTACCTTCAACAACCAAAGATTGCATCTGTCATGACAACTTAAGGGCATCTTCTACGTTATCGAGTTACGAAAATCATCCGATAGAGATACTGCCTTCAAAATGTAACTCTCTTGACGAAGCCACAGGTAAAATATATCTCATGGCTAAAGATCAACATGGTTGTCGCTTCTTACAACGGATGTTCACTGACGGTACTAAAGAAGATATCGAGATGATTTTCGGTGAGATCATTCATCATGTTTCTGAGCTCATGATAGACCCTTTTGGAAACTATTTGATACAGAAACTCCTTGGAGTTTGTGACGAGGATCAACGCCTTCAAATTCTTTATAAGATTAACCGACCCGGTGAACTGATTCGAATCTCGTGTAATATGCATGGGACTCGTGCCGTTCAGAAGCTCATCGAGACTCTCAAAACGCAAGAGCAGTTTTCCATGGTTGTCTCTTTATTGAAGACTGGTATAGTGATACTCATGAAGAATGTTAATGGTAACCATGTAGCACAGCATTGCCTGCAGTATTTGATGCCTGATTATATTGGTTTTCTTTTTGATGCTGCAATAAAATCTTGTGTCGAGGTTGCAACTGATCGCCACGGTTGTTGCGTGCTTCAAAAGTGCCTTTCTGTTTCTGATGCTCGATACCGAGACCGTTTGTTAAGCGAGGTCGTACGTAATGCTTTGGTCCTTTCCCAAGATCAGTACGGGAACTATGTCGTGCAATTCTCCCTTGAGCTTGGGCGCTGTCCTACGAGCATTCCTTGGGTGACGCCTGGTATCTTCAAACGACTAGAGGGCCATTTTGCAGACTTGTCTATCCAGAAATATAGCAGTAATGTTGTTGAAAAATGTGCTTACACTGGAGAAGATTATCTCTTTAAGATTATCGACGAGTTGATAAACGACGAACGATTCAGTCAAATTATGCTGAACCCATATGGAAACTATGCAGTTCAAGCTGTTCTTGCTCGTTCTTGGAATTTCAAAAGTCCTGTTCATGCTAAACTTGTGGCTGCTATCAGACCGCACGTCCCGTTGCTCAGGACGAACATGGAGCTTAGGCCATTAACCATGGCTGCAACTGTGCAACATGGTTCGTCTCCATATCTCGAAAGAAAACCAATCGGAGTTGAAGACGATCATCCATCACAATCCGTTCCAACAAATGGTAACATGTCTGTATTTTCCTTTGTAGATTCGCTGTTGTATTGGCGACCGAGATGA

Coding sequence (CDS)

ATGGGCAGTAAACTGAAGCTTGAACTTGAGAAGCTTTTCAGTGAGGATCTGATAGTTGCTTGTGACAATGAACATGCTGAAGAATCTGTTCATAAAGGAATGTCTTCAATGGTTTCAGATTCTTGTAATGGGTTGTCCTTGGATGAACTCCACAATAATGGGAGTTTGGAGAATGGAAAGGTCTTGATCGGTCGATTTGGAGACCGATCGTTGACATCTGCAGTCGAGAAACTAAGTTTAGGGGGGGAGGGGACAGGGAAGACTTGGATGAATCACCCCAATTTGATGGATGATCAATTTGAAAGCTATTTGAATAAACAATCACTCAAAACTGAACCATCAATGGTGAGTGATCCCCCTTTGAGACCTTCACATTGTGTGAACAATGGCTATTATGAGATTCGTATGCCGGGTCTTCCCCCTCAGATCTCGGTCGAAGCAAGACCTGTTTCTGATGTCCACAAGAATGGGGAGGCGGGTCAGGTTCCGCCATTGAAGACTCCTGCTGCTATGCCATTCACTCGTCATGAACAAATGTTGCACAGTGGGTTATCCCCAATTCATTTTATGCGCCCACAACAAATGAATCATGGTGAAATAGGTTCAAATTGTACCAAAGAACAGCAGCTCCTAAGCTGCAGGATGCAAGGGCAGCACCAATACCTGTATGATCTTCATAATCAGCAACGGGAATATTCGAATCTATTTGAATCTTGTGGTAAACCGTTTGGGTCGTTGCGGTTTCGGAGTCCGAATCAGGAATGTTTTATTGAGGTTCCCTTTTCTCCCTATCTTGGGCAGTTTAAACATGAAGGATTCTGCAATAGGTCTGCACATTATGCAGGTTCGGGCGTACCAAATCTTGTATTTACAAAACCGAACATAGATACGTTAGATTCCCAGGAAAAAAGTTTCAATCAAAGCTCTCCTAGGAAGGTCCCCATGAGGACACATGGACCGATTGGAGTTGATTGTATGAAGGCTCCGATCGGAGTTGATGCTGAGAAACTTAAGTATTATATCAGCCAAAATGGATTTGGTTGTCCAAGTTGTATTGTTAGGCAGAATGGTTTACCTTCAACAACCAAAGATTGCATCTGTCATGACAACTTAAGGGCATCTTCTACGTTATCGAGTTACGAAAATCATCCGATAGAGATACTGCCTTCAAAATGTAACTCTCTTGACGAAGCCACAGGTAAAATATATCTCATGGCTAAAGATCAACATGGTTGTCGCTTCTTACAACGGATGTTCACTGACGGTACTAAAGAAGATATCGAGATGATTTTCGGTGAGATCATTCATCATGTTTCTGAGCTCATGATAGACCCTTTTGGAAACTATTTGATACAGAAACTCCTTGGAGTTTGTGACGAGGATCAACGCCTTCAAATTCTTTATAAGATTAACCGACCCGGTGAACTGATTCGAATCTCGTGTAATATGCATGGGACTCGTGCCGTTCAGAAGCTCATCGAGACTCTCAAAACGCAAGAGCAGTTTTCCATGGTTGTCTCTTTATTGAAGACTGGTATAGTGATACTCATGAAGAATGTTAATGGTAACCATGTAGCACAGCATTGCCTGCAGTATTTGATGCCTGATTATATTGGTTTTCTTTTTGATGCTGCAATAAAATCTTGTGTCGAGGTTGCAACTGATCGCCACGGTTGTTGCGTGCTTCAAAAGTGCCTTTCTGTTTCTGATGCTCGATACCGAGACCGTTTGTTAAGCGAGGTCGTACGTAATGCTTTGGTCCTTTCCCAAGATCAGTACGGGAACTATGTCGTGCAATTCTCCCTTGAGCTTGGGCGCTGTCCTACGAGCATTCCTTGGGTGACGCCTGGTATCTTCAAACGACTAGAGGGCCATTTTGCAGACTTGTCTATCCAGAAATATAGCAGTAATGTTGTTGAAAAATGTGCTTACACTGGAGAAGATTATCTCTTTAAGATTATCGACGAGTTGATAAACGACGAACGATTCAGTCAAATTATGCTGAACCCATATGGAAACTATGCAGTTCAAGCTGTTCTTGCTCGTTCTTGGAATTTCAAAAGTCCTGTTCATGCTAAACTTGTGGCTGCTATCAGACCGCACGTCCCGTTGCTCAGGACGAACATGGAGCTTAGGCCATTAACCATGGCTGCAACTGTGCAACATGGTTCGTCTCCATATCTCGAAAGAAAACCAATCGGAGTTGAAGACGATCATCCATCACAATCCGTTCCAACAAATGGTAACATGTCTGTATTTTCCTTTGTAGATTCGCTGTTGTATTGGCGACCGAGATGA

Protein sequence

MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGKVLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPPLRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQMLHSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCGKPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDSQEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPSTTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRISCNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELINDERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNMELRPLTMAATVQHGSSPYLERKPIGVEDDHPSQSVPTNGNMSVFSFVDSLLYWRPR
Homology
BLAST of CmaCh10G008100 vs. ExPASy Swiss-Prot
Match: Q9LVC3 (Pumilio homolog 12 OS=Arabidopsis thaliana OX=3702 GN=APUM12 PE=2 SV=2)

HSP 1 Score: 378.3 bits (970), Expect = 2.1e-103
Identity = 191/326 (58.59%), Postives = 244/326 (74.85%), Query Frame = 0

Query: 385 IEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMID 444
           + + P K NS+ EA GKIY +AKDQHGCRFLQR+F++    DIEMIF EII ++SELM+D
Sbjct: 265 LSLQPQKYNSIAEARGKIYYLAKDQHGCRFLQRIFSEKDGNDIEMIFNEIIDYISELMMD 324

Query: 445 PFGNYLIQKLLGVCDEDQRLQILYKINR-PGELIRISCNMHGTRAVQKLIETLKTQEQFS 504
           PFGNYL+QKLL VC+EDQR+QI++ I R PG LI+ISC+MHGTRAVQK++ET K +E+ S
Sbjct: 325 PFGNYLVQKLLEVCNEDQRMQIVHSITRKPGLLIKISCDMHGTRAVQKIVETAKREEEIS 384

Query: 505 MVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQ 564
           +++S LK GIV L+KNVNGNHV Q CLQYL+P    FLF+AAI  CVE+ATDRHGCCVLQ
Sbjct: 385 IIISALKHGIVHLIKNVNGNHVVQRCLQYLLPYCGKFLFEAAITHCVELATDRHGCCVLQ 444

Query: 565 KCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLE 624
           KCL  S+   +  L+SE+  NAL+LSQD +GNYV+Q+  EL      + W T  I ++LE
Sbjct: 445 KCLGYSEGEQKQHLVSEIASNALLLSQDPFGNYVLQYVFEL-----QLQWATFEILEQLE 504

Query: 625 GHFADLSIQKYSSNVVEKCAYTGED-YLFKIIDELINDERFSQIMLNPYGNYAVQAVLAR 684
           G++ +LS+QK SSNVVEKC    +D +  +II ELIN  R  Q+ML+PYGNY +QA L +
Sbjct: 505 GNYTELSMQKCSSNVVEKCLKLADDKHRARIIRELINYGRLDQVMLDPYGNYVIQAALKQ 564

Query: 685 SWNFKSPVHAKLVAAIRPHVPLLRTN 709
           S   K  VHA LV AI+ ++  LRTN
Sbjct: 565 S---KGNVHALLVDAIKLNISSLRTN 582

BLAST of CmaCh10G008100 vs. ExPASy Swiss-Prot
Match: Q9C9R6 (Putative pumilio homolog 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APUM7 PE=3 SV=2)

HSP 1 Score: 322.4 bits (825), Expect = 1.4e-86
Identity = 165/342 (48.25%), Postives = 233/342 (68.13%), Query Frame = 0

Query: 369 DNLRASS-TLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDI 428
           D+ R+++  L S   +P EI      ++ +  G +YLMAKDQHGCRFLQR+F +GT  D 
Sbjct: 304 DHQRSNTRALMSNNGNPTEICHPSLPNMCDIQGYVYLMAKDQHGCRFLQRIFDEGTSVDA 363

Query: 429 EMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQI-LYKINRPGELIRISCNMHGT 488
            +IF E+I HV ELM+DPFGNYL+QKLL VC E+QR QI L     PG+LIRIS N +GT
Sbjct: 364 MIIFNEVIAHVVELMMDPFGNYLMQKLLDVCTEEQRTQIVLVATEEPGQLIRISLNAYGT 423

Query: 489 RAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAI 548
           R VQ+L+ET+++ +Q S+V   L+ G + L+K++NGNHV Q CLQ L  +   F+FDAA 
Sbjct: 424 RVVQRLVETIRSGKQISLVKLALRPGFLDLIKDLNGNHVIQRCLQCLSTEDNKFIFDAAT 483

Query: 549 KSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGR 608
           K C E+AT RHGCCVLQKC++ S  + R++L++E+ RN+L+L+QD +GNY VQF +EL  
Sbjct: 484 KFCTEIATHRHGCCVLQKCIAYSMRQQREKLIAEISRNSLLLAQDPFGNYAVQFVIEL-- 543

Query: 609 CPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELINDERFSQI 668
               IP     +  +L+GH+  LS+QK+SS++VE+C     +   +I+ EL++   F Q+
Sbjct: 544 ---RIPSAVAMMLAQLKGHYVQLSMQKFSSHMVERCLMHCPESRPQIVRELVSVPHFDQL 603

Query: 669 MLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTN 709
           + +PY N+ +QA LA +   K P+HA LV  IRPH  +LR N
Sbjct: 604 LQDPYANFVIQAALAAT---KGPLHASLVEVIRPH-SILRNN 636

BLAST of CmaCh10G008100 vs. ExPASy Swiss-Prot
Match: Q9LM20 (Putative pumilio homolog 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APUM8 PE=3 SV=2)

HSP 1 Score: 308.5 bits (789), Expect = 2.1e-82
Identity = 170/379 (44.85%), Postives = 244/379 (64.38%), Query Frame = 0

Query: 331 GVDAEKLKYYISQNGFGCPSCIVRQNGLPSTTKDCICHDNLRASSTLSSYENHPIEILPS 390
           GVD +  +   S N  G  SC +    +P   K       + A        ++P E LP 
Sbjct: 142 GVDQKDHQISNSWN-LGMQSCNMNNYSVPVHGK-----SGVGALFDHQGISSNPNESLP- 201

Query: 391 KCNSLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMIDPFGNYL 450
               + E  G +Y MAKDQHGCRFLQ +F DG+  D  +IF E+I HV ELM+DPFGNYL
Sbjct: 202 ---KVSEFQGYVYFMAKDQHGCRFLQWIFEDGSALDALVIFSEVIPHVVELMMDPFGNYL 261

Query: 451 IQKLLGVCDEDQRLQILYKI-NRPGELIRISCNMHGTRAVQKLIETLKTQEQFSMVVSLL 510
           +QKLL VC+E+QR QI+  + + PG+LIRIS N +GTR VQ+L+E++KT++Q S+V S L
Sbjct: 262 MQKLLDVCNEEQRTQIILMVTSEPGQLIRISLNAYGTRVVQRLVESIKTRKQISLVKSAL 321

Query: 511 KTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQKCLSVS 570
           + G + L++++NGNHV Q CLQ L  +   F+F+ A K C+++AT RHGCCVLQKC++ S
Sbjct: 322 RPGFLNLIRDLNGNHVIQRCLQCLSTEDNEFIFEDATKFCIDIATHRHGCCVLQKCIAYS 381

Query: 571 DARYRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLEGHFADL 630
               R++L++E+ RN+L L+QD YGNY VQF LEL R  ++I      +  +L+GH+ +L
Sbjct: 382 SGLQREKLVTEISRNSLFLAQDPYGNYAVQFVLEL-RDFSAI----AAMLAQLKGHYVEL 441

Query: 631 SIQKYSSNVVEKCAYTGEDYLFKIIDELINDERFSQIMLNPYGNYAVQAVLARSWNFKSP 690
           S+QK+SS++VE+C     +   +I+ ELI+   F  ++ +PY N+ +QA LA +   K  
Sbjct: 442 SMQKFSSHMVERCLTHCPESRPQIVRELISVPHFDILIQDPYANFVIQAALAVT---KGS 501

Query: 691 VHAKLVAAIRPHVPLLRTN 709
           +HA LV  IRPH  +LR N
Sbjct: 502 LHATLVEVIRPH-SILRNN 501

BLAST of CmaCh10G008100 vs. ExPASy Swiss-Prot
Match: Q9LDW3 (Pumilio homolog 11 OS=Arabidopsis thaliana OX=3702 GN=APUM11 PE=3 SV=2)

HSP 1 Score: 270.8 bits (691), Expect = 4.8e-71
Identity = 138/315 (43.81%), Postives = 208/315 (66.03%), Query Frame = 0

Query: 394 SLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQK 453
           S+ ++ G +YLMAKDQ GCR LQ+   +G   D+ +IF E+I++V EL  DPFGNYLIQK
Sbjct: 238 SMVDSYGSVYLMAKDQLGCRLLQKFVDEGNFVDVMIIFKEVINNVIELGTDPFGNYLIQK 297

Query: 454 LLGVCDEDQRLQILYKI-NRPGELIRISCNMHGTRAVQKLIETLKTQEQFSMVVSLLKTG 513
           L+ VC+E+QR QIL ++ ++PG L++IS N +GTR VQKLIET+ T+EQ S+V S L  G
Sbjct: 298 LIEVCNEEQRTQILIRLTSKPGLLVKISINNYGTRVVQKLIETVTTKEQISLVKSALVPG 357

Query: 514 IVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQKCLSVSDAR 573
            + L + +NGNHV  +CL++  P+   F+ +AA K C+E+AT RHGCCVLQ+C+S S   
Sbjct: 358 FLSLFRELNGNHVILNCLKFFSPNDNKFILEAATKFCIEIATTRHGCCVLQRCVSYSVGE 417

Query: 574 YRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLEGHFADLSIQ 633
             ++L+ E+ RN+L+L+QD +GNY+VQ+ +E       +  V   +   L G++  L+ Q
Sbjct: 418 QHEKLVDEISRNSLLLAQDPFGNYLVQYIIE-----KKVGGV--NVLFELRGNYVKLATQ 477

Query: 634 KYSSNVVEKCAYTGEDYLFKIIDELINDERFSQIMLNPYGNYAVQAVLARSWNFKSPVHA 693
           K+ S+VVEKC     +   +I++EL++   F  ++ +PY NY +Q  L+++  F   V A
Sbjct: 478 KFGSHVVEKCLRYYPESRSQIVNELVSVLNFGYLLQDPYANYVIQCALSKTKGF---VRA 537

Query: 694 KLVAAIRPHVPLLRT 708
            LV  +R +  L  T
Sbjct: 538 SLVEKVRRYENLKMT 542

BLAST of CmaCh10G008100 vs. ExPASy Swiss-Prot
Match: Q1PFN9 (Pumilio homolog 9 OS=Arabidopsis thaliana OX=3702 GN=APUM9 PE=2 SV=1)

HSP 1 Score: 261.9 bits (668), Expect = 2.2e-68
Identity = 139/306 (45.42%), Postives = 198/306 (64.71%), Query Frame = 0

Query: 394 SLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQK 453
           S+ E  G + LMAKDQ GCR LQ++  +GT  + ++I   II HV EL +DPFGNY++QK
Sbjct: 246 SMVEIYGSVNLMAKDQIGCRVLQKLVEEGTFHEAKVILLAIIDHVVELSMDPFGNYIVQK 305

Query: 454 LLGVCDEDQRLQILYKI-NRPGELIRISCNMHGTRAVQKLIETLKTQEQFSMVVSLLKTG 513
           L  V DE+QR  I+  + + P ELIRI  N +GTR VQK+IET+KT++Q ++V S LK G
Sbjct: 306 LFDVSDEEQRTLIVSVLTSNPRELIRICLNTYGTRVVQKMIETVKTKQQIALVKSGLKPG 365

Query: 514 IVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQKCLSVSDAR 573
            + L+K++NGNHV Q CLQ L P+   F+ +AA K C E+A  RHGCCVLQ C+S S   
Sbjct: 366 FLALVKDLNGNHVIQSCLQTLGPNDNEFVLEAATKYCAEIAIHRHGCCVLQCCISNSVGL 425

Query: 574 YRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLEGHFADLSIQ 633
            R+RL++E+ RN+L LSQD +GNYVVQ+ ++              +  +   H+A+L+ Q
Sbjct: 426 QRERLVAEISRNSLHLSQDPFGNYVVQYLIDQQVSAVK-------LLVQFRMHYAELATQ 485

Query: 634 KYSSNVVEKCAYTGEDYLFKIIDELINDERFSQIMLNPYGNYAVQAVLARSWNFKSPVHA 693
           K+SS+V+EKC     +   +I+ EL+    F  ++ +PY NY +Q  L+ +   K PV A
Sbjct: 486 KFSSHVIEKCLRKYPESRAEIVRELLCVPNFEYLLQDPYANYVIQTALSVT---KGPVRA 541

Query: 694 KLVAAI 699
           KLVA +
Sbjct: 546 KLVAKV 541

BLAST of CmaCh10G008100 vs. ExPASy TrEMBL
Match: A0A6J1JGR0 (putative pumilio homolog 8, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC111484955 PE=4 SV=1)

HSP 1 Score: 1464.9 bits (3791), Expect = 0.0e+00
Identity = 709/709 (100.00%), Postives = 709/709 (100.00%), Query Frame = 0

Query: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60
           MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK
Sbjct: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60

Query: 61  VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120
           VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP
Sbjct: 61  VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120

Query: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML 180
           LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML
Sbjct: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML 180

Query: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG 240
           HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG
Sbjct: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG 240

Query: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS 300
           KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS
Sbjct: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS 300

Query: 301 QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS 360
           QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS
Sbjct: 301 QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS 360

Query: 361 TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420
           TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT
Sbjct: 361 TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420

Query: 421 DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480
           DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS
Sbjct: 421 DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480

Query: 481 CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540
           CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF
Sbjct: 481 CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540

Query: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600
           LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF
Sbjct: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600

Query: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660
           SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND
Sbjct: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660

Query: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM 710
           ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM
Sbjct: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM 709

BLAST of CmaCh10G008100 vs. ExPASy TrEMBL
Match: A0A6J1HA05 (putative pumilio homolog 8, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC111461461 PE=4 SV=1)

HSP 1 Score: 1431.0 bits (3703), Expect = 0.0e+00
Identity = 694/709 (97.88%), Postives = 699/709 (98.59%), Query Frame = 0

Query: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60
           MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELH NGSLENGK
Sbjct: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHKNGSLENGK 60

Query: 61  VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120
           V IG+FGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP
Sbjct: 61  VSIGQFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120

Query: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML 180
           LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAG VPPLKTP+AMPFT HEQML
Sbjct: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGHVPPLKTPSAMPFTHHEQML 180

Query: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG 240
           HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNL ESCG
Sbjct: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLLESCG 240

Query: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS 300
           KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNR+AHYAGSGVPNLVFTKPN+DTLDS
Sbjct: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRTAHYAGSGVPNLVFTKPNVDTLDS 300

Query: 301 QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS 360
           QEKSFNQ S RKVPMRTHGPIGVDCMKAPIGVDAEKLKYYIS NGFGCPSCIVRQNGLPS
Sbjct: 301 QEKSFNQCSLRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYIS-NGFGCPSCIVRQNGLPS 360

Query: 361 TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420
           TTKDCICHDNLRASSTLSSYENHPIEI PSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT
Sbjct: 361 TTKDCICHDNLRASSTLSSYENHPIEIPPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420

Query: 421 DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480
           +GTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS
Sbjct: 421 EGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480

Query: 481 CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540
           CNMHGTRAVQKLIETLKT EQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF
Sbjct: 481 CNMHGTRAVQKLIETLKTPEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540

Query: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600
           LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF
Sbjct: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600

Query: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660
           SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND
Sbjct: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660

Query: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM 710
           ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM
Sbjct: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM 708

BLAST of CmaCh10G008100 vs. ExPASy TrEMBL
Match: A0A0A0LZQ0 (PUM-HD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G599460 PE=4 SV=1)

HSP 1 Score: 1106.7 bits (2861), Expect = 0.0e+00
Identity = 556/741 (75.03%), Postives = 609/741 (82.19%), Query Frame = 0

Query: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60
           MGSKLK ELE+  SE+ IVAC+N  AEESV KG+ SM +DSC+GLSL++LH+NGSLENG 
Sbjct: 1   MGSKLKFELERFLSENPIVACENVFAEESVQKGVPSMGADSCSGLSLEKLHSNGSLENGP 60

Query: 61  VLIGRF----------------GDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYL 120
           VLI  F                GDRSLTSAVEKLSLGGEGTG+TWMNHPNLMDDQFESY+
Sbjct: 61  VLINEFQQSSTGQFRTGESNRIGDRSLTSAVEKLSLGGEGTGQTWMNHPNLMDDQFESYM 120

Query: 121 NKQSLKTEPSMVSDPPLRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPP 180
           NKQS+ +E S V++P LR S+ V+NGYYEI +PGL PQ+S  AR VSDV K G+A  + P
Sbjct: 121 NKQSINSEASTVANPSLRSSNRVSNGYYEIPVPGLSPQLSFSARLVSDVQKKGDAAHLTP 180

Query: 181 LKTP-AAMPFT--------------RHEQMLHSGLSPIHFMRPQQMNHGEIGSNCTKEQQ 240
            +TP +AMPFT               H+QML +GLSP+HFM PQQMNHGEIG N  K +Q
Sbjct: 181 FETPNSAMPFTHEVPARNLQFSLPSHHDQMLLNGLSPVHFMHPQQMNHGEIGPNSVKGEQ 240

Query: 241 LLSCRMQGQHQYLYDLHNQQREYSNLFESCGK-PFGSLRFRSPNQECFIEVPFSPYLGQF 300
           L SCRMQ Q QYL+DLHNQQ E SNLFESCG   FGSLRF+SP Q+ F+EVPF P   Q 
Sbjct: 241 LHSCRMQWQQQYLHDLHNQQLERSNLFESCGNAAFGSLRFQSPKQQRFVEVPFHPCREQS 300

Query: 301 KHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDSQEKSFNQSSPRKVPMRTHGPIGVDCMKA 360
           KHEGFCN +AH A SG+PN  F  P +DTLD+QEK F QSSPRK+P R HG         
Sbjct: 301 KHEGFCNGAAHCAASGIPNHAFATPFLDTLDAQEKCFKQSSPRKIPTRAHG--------- 360

Query: 361 PIGVDAEKLKYYISQNGFGCPSCIVRQNGLPSTTKDCICHDNLRASSTLSSYENHPIEIL 420
            IGVD EKLKYY SQNGF CPSC VRQ G PST KDCICHDN R SS LSS+ N   EI 
Sbjct: 361 LIGVDTEKLKYYFSQNGFLCPSCYVRQYGFPSTAKDCICHDNFRVSSMLSSHANRKTEIP 420

Query: 421 PSKCNSLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMIDPFGN 480
           P KCNSLDEATGKIYLMAKDQHGCRFLQRMF++GTKEDIEMIFGEIIHHVSELM+DPFGN
Sbjct: 421 PLKCNSLDEATGKIYLMAKDQHGCRFLQRMFSEGTKEDIEMIFGEIIHHVSELMVDPFGN 480

Query: 481 YLIQKLLGVCDEDQRLQILYKINRPGELIRISCNMHGTRAVQKLIETLKTQEQFSMVVSL 540
           YLIQKLLGVCDEDQRLQILYKINRPGELIRISCNMHGTRAVQKLIETLKT EQFS++VSL
Sbjct: 481 YLIQKLLGVCDEDQRLQILYKINRPGELIRISCNMHGTRAVQKLIETLKTPEQFSLIVSL 540

Query: 541 LKTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQKCLSV 600
           LKTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAA KSCVEVATDRHGCCVLQKCL+V
Sbjct: 541 LKTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAATKSCVEVATDRHGCCVLQKCLAV 600

Query: 601 SDARYRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLEGHFAD 660
           SDAR+RDRLLSEVVRNALVLSQDQYGNYVVQF+LEL RCP+ +PWVT GIFKRLEGHF+D
Sbjct: 601 SDARHRDRLLSEVVRNALVLSQDQYGNYVVQFALELARCPSILPWVTSGIFKRLEGHFSD 660

Query: 661 LSIQKYSSNVVEKCAYTGEDYLFKIIDELINDERFSQIMLNPYGNYAVQAVLARSWNFKS 710
           LSIQKYSSNVVE+C Y G++YL K++DELINDERFSQIMLNPYGNYAVQAVLARS   KS
Sbjct: 661 LSIQKYSSNVVERCVYAGDEYLAKVVDELINDERFSQIMLNPYGNYAVQAVLARSGICKS 720

BLAST of CmaCh10G008100 vs. ExPASy TrEMBL
Match: A0A5A7UN64 (Pumilio-like protein 12-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 GN=E5676_scaffold16G001060 PE=4 SV=1)

HSP 1 Score: 1100.9 bits (2846), Expect = 0.0e+00
Identity = 552/740 (74.59%), Postives = 605/740 (81.76%), Query Frame = 0

Query: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60
           MGSKLK ELE+  SE+ IVAC+N  AEESV KG+SSM +DSC GLSL++LH+N SLENG 
Sbjct: 1   MGSKLKFELERFLSENPIVACENVFAEESVQKGVSSMTADSCTGLSLEKLHSNRSLENGP 60

Query: 61  VLIGRF----------------GDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYL 120
           VLI  F                GDRSLTSAVEKLSLGGEGTG+TWMNHPNLM+DQFESY+
Sbjct: 61  VLINEFQQSSTGQFLTGESNRIGDRSLTSAVEKLSLGGEGTGQTWMNHPNLMNDQFESYM 120

Query: 121 NKQSLKTEPSMVSDPPLRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPP 180
           NKQS+ +EPS V++P LR S+ V+NGYYEI +PGL PQ+S  AR VSDV K GEAG + P
Sbjct: 121 NKQSINSEPSTVANPSLRSSNRVSNGYYEISVPGLSPQLSFPARFVSDVQKKGEAGHLTP 180

Query: 181 LKTP-AAMPFT--------------RHEQMLHSGLSPIHFMRPQQMNHGEIGSNCTKEQQ 240
            +TP +AMPFT               H+QML +GLSP+HFM  QQMNHGEIG NC K +Q
Sbjct: 181 FETPNSAMPFTNEVPARNLQFPLSSHHDQMLLNGLSPVHFMHSQQMNHGEIGPNCAKGEQ 240

Query: 241 LLSCRMQGQHQYLYDLHNQQREYSNLFESCGKPFGSLRFRSPNQECFIEVPFSPYLGQFK 300
           L SCRMQ Q QYL+DLHNQQ E SNLFESCG     LRF+SP Q+ F+EVPF P   Q K
Sbjct: 241 LHSCRMQWQQQYLHDLHNQQLERSNLFESCGNAAFGLRFQSPKQQRFVEVPFHPCREQSK 300

Query: 301 HEGFCNRSAHYAGSGVPNLVFTKPNIDTLDSQEKSFNQSSPRKVPMRTHGPIGVDCMKAP 360
           HEGFCN +AH A SG+PN  F  P +DTLD+QEK F QSSPRKVP R HG          
Sbjct: 301 HEGFCNGAAHCAASGIPNHAFASPYLDTLDAQEKCFKQSSPRKVPTRAHG---------L 360

Query: 361 IGVDAEKLKYYISQNGFGCPSCIVRQNGLPSTTKDCICHDNLRASSTLSSYENHPIEILP 420
           IGVD EKLKYY SQNGF CPSC VRQ G PST KDCICHDN R  S LSS  N   EI P
Sbjct: 361 IGVDTEKLKYYFSQNGFLCPSCYVRQYGFPSTAKDCICHDNFRVPSMLSSNANRNTEIPP 420

Query: 421 SKCNSLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMIDPFGNY 480
            KCNSLDEA+GKIYLMAKDQHGCRFLQRMF++GTKEDIE+IFGEIIHHVSELM+DPFGNY
Sbjct: 421 LKCNSLDEASGKIYLMAKDQHGCRFLQRMFSEGTKEDIEIIFGEIIHHVSELMVDPFGNY 480

Query: 481 LIQKLLGVCDEDQRLQILYKINRPGELIRISCNMHGTRAVQKLIETLKTQEQFSMVVSLL 540
           LIQKLLGVCDEDQRLQILYKINRPGELIRISCNMHGTRAVQKLIETLKT EQFS++VSLL
Sbjct: 481 LIQKLLGVCDEDQRLQILYKINRPGELIRISCNMHGTRAVQKLIETLKTPEQFSLIVSLL 540

Query: 541 KTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQKCLSVS 600
           KTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLF+AA KSCVEVATDRHGCCVLQKCL+VS
Sbjct: 541 KTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFEAATKSCVEVATDRHGCCVLQKCLAVS 600

Query: 601 DARYRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLEGHFADL 660
           DAR+RDRLLSEVVRNALVLSQDQYGNYVVQF+LEL RCP+ +PWVT GIFKRLEGHF+DL
Sbjct: 601 DARHRDRLLSEVVRNALVLSQDQYGNYVVQFALELARCPSMLPWVTSGIFKRLEGHFSDL 660

Query: 661 SIQKYSSNVVEKCAYTGEDYLFKIIDELINDERFSQIMLNPYGNYAVQAVLARSWNFKSP 710
           SIQKYSSNVVE+C Y G++YL K++DELINDERFSQIMLNPYGNYAVQAVLARS   KS 
Sbjct: 661 SIQKYSSNVVERCVYAGDEYLAKVVDELINDERFSQIMLNPYGNYAVQAVLARSGICKSS 720

BLAST of CmaCh10G008100 vs. ExPASy TrEMBL
Match: A0A1S4DY70 (uncharacterized protein LOC103492417 OS=Cucumis melo OX=3656 GN=LOC103492417 PE=4 SV=1)

HSP 1 Score: 1093.2 bits (2826), Expect = 0.0e+00
Identity = 549/740 (74.19%), Postives = 603/740 (81.49%), Query Frame = 0

Query: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60
           MGSKLK ELE+  SE+ IVAC+N  AE+SV KG+SSM +DSC GLSL++LH+NGSLENG 
Sbjct: 1   MGSKLKFELERFLSENPIVACENVFAEKSVQKGVSSMTADSCTGLSLEKLHSNGSLENGP 60

Query: 61  VLIGRF----------------GDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYL 120
           VLI  F                G RSLTSAVEKLSLGGEGTG+TWMNHPNLM+DQFESY+
Sbjct: 61  VLINEFQQSSTGQFLTGESNRIGVRSLTSAVEKLSLGGEGTGQTWMNHPNLMNDQFESYM 120

Query: 121 NKQSLKTEPSMVSDPPLRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPP 180
           NKQS+ +EPS V++P LR S+ V+NGYYEI +PGL PQ+S  AR VSDV K GEAG + P
Sbjct: 121 NKQSINSEPSTVANPSLRSSNRVSNGYYEISVPGLSPQLSFPARFVSDVQKKGEAGHLTP 180

Query: 181 LKTP-AAMPFT--------------RHEQMLHSGLSPIHFMRPQQMNHGEIGSNCTKEQQ 240
            +TP +AMPFT                +QML +GLSP+HFM  QQMNHGEIG NC K +Q
Sbjct: 181 FETPNSAMPFTNEVPARNLQFPLSSHRDQMLLNGLSPVHFMHSQQMNHGEIGPNCAKGEQ 240

Query: 241 LLSCRMQGQHQYLYDLHNQQREYSNLFESCGKPFGSLRFRSPNQECFIEVPFSPYLGQFK 300
           L SCRMQ Q QYL+DLHNQQ E SNLFESCG     LRF+SP Q+ F+EVPF P   Q K
Sbjct: 241 LHSCRMQWQQQYLHDLHNQQLERSNLFESCGNAAFGLRFQSPKQQRFVEVPFHPCREQSK 300

Query: 301 HEGFCNRSAHYAGSGVPNLVFTKPNIDTLDSQEKSFNQSSPRKVPMRTHGPIGVDCMKAP 360
           HEGFCN +AH A SG+PN  F  P +DTLD+QEK F QSSPRKVP R HG          
Sbjct: 301 HEGFCNGAAHCAASGIPNHAFASPYLDTLDAQEKCFKQSSPRKVPTRAHG---------L 360

Query: 361 IGVDAEKLKYYISQNGFGCPSCIVRQNGLPSTTKDCICHDNLRASSTLSSYENHPIEILP 420
           IGVD EKLKYY SQNGF CPSC VRQ G PST KDCICHDN R  S LSS  N   EI P
Sbjct: 361 IGVDTEKLKYYFSQNGFLCPSCYVRQYGFPSTAKDCICHDNFRVPSMLSSNANRNTEIPP 420

Query: 421 SKCNSLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMIDPFGNY 480
            KCNSLDEA+GKIYLMAKDQHGCRFLQRMF++GTKEDIE+IFGEIIHHVSELM+DPFGNY
Sbjct: 421 LKCNSLDEASGKIYLMAKDQHGCRFLQRMFSEGTKEDIEIIFGEIIHHVSELMVDPFGNY 480

Query: 481 LIQKLLGVCDEDQRLQILYKINRPGELIRISCNMHGTRAVQKLIETLKTQEQFSMVVSLL 540
           LIQKLLGVCDEDQRLQILYKINRPGELIRISCNMHGTRAVQKLIETLKT EQFS++VSLL
Sbjct: 481 LIQKLLGVCDEDQRLQILYKINRPGELIRISCNMHGTRAVQKLIETLKTPEQFSLIVSLL 540

Query: 541 KTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQKCLSVS 600
           KTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLF+AA KSCVEVATDRHGCCVLQKCL+VS
Sbjct: 541 KTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFEAATKSCVEVATDRHGCCVLQKCLAVS 600

Query: 601 DARYRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLEGHFADL 660
           DA +RDRLLSEVVRNALVLSQDQYGNYVVQF+LEL RCP+ +PWVT GIFKRLEGHF+DL
Sbjct: 601 DAGHRDRLLSEVVRNALVLSQDQYGNYVVQFALELARCPSMLPWVTSGIFKRLEGHFSDL 660

Query: 661 SIQKYSSNVVEKCAYTGEDYLFKIIDELINDERFSQIMLNPYGNYAVQAVLARSWNFKSP 710
           SIQKYSSNVVE+C Y G++YL K++DELINDERFSQIMLNPYGNYAVQAVLARS   KS 
Sbjct: 661 SIQKYSSNVVERCVYAGDEYLAKVVDELINDERFSQIMLNPYGNYAVQAVLARSGICKSS 720

BLAST of CmaCh10G008100 vs. NCBI nr
Match: KAG6590074.1 (Pumilio-like 12, partial [Cucurbita argyrosperma subsp. sororia])

HSP 1 Score: 1548.1 bits (4007), Expect = 0.0e+00
Identity = 750/765 (98.04%), Postives = 755/765 (98.69%), Query Frame = 0

Query: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60
           MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK
Sbjct: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60

Query: 61  VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120
           V IG+FGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP
Sbjct: 61  VSIGQFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120

Query: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML 180
           LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAG VPPLKTP+AMPFT HEQML
Sbjct: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGHVPPLKTPSAMPFTHHEQML 180

Query: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG 240
           HSGLSPIHFMRPQ MNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNL ESCG
Sbjct: 181 HSGLSPIHFMRPQHMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLLESCG 240

Query: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS 300
           KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNR+AHYAGSGVPNLVFTKPN+DTLDS
Sbjct: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRTAHYAGSGVPNLVFTKPNVDTLDS 300

Query: 301 QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS 360
           QEKSFNQ SPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYIS NGFGCPSCIVRQNGLPS
Sbjct: 301 QEKSFNQCSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYIS-NGFGCPSCIVRQNGLPS 360

Query: 361 TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420
           TTKDCICHDNLRASSTLSSYENHPIEI PSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT
Sbjct: 361 TTKDCICHDNLRASSTLSSYENHPIEIPPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420

Query: 421 DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480
           +GTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS
Sbjct: 421 EGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480

Query: 481 CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540
           CNMHGTRAVQKLIETLKT EQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF
Sbjct: 481 CNMHGTRAVQKLIETLKTPEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540

Query: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600
           LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF
Sbjct: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600

Query: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660
           SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND
Sbjct: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660

Query: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNMELRPLTMAATV 720
           ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNMELRPLTMAATV
Sbjct: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNMELRPLTMAATV 720

Query: 721 QHGSSPYLERKPIGVEDDHPSQSVPTNGNMSVFSFVDSLLYWRPR 766
           QHGSSPYLERKPIGVEDDHPSQSV TNGNMSVFSFVDSLLYWRPR
Sbjct: 721 QHGSSPYLERKPIGVEDDHPSQSVATNGNMSVFSFVDSLLYWRPR 764

BLAST of CmaCh10G008100 vs. NCBI nr
Match: XP_022987390.1 (putative pumilio homolog 8, chloroplastic [Cucurbita maxima])

HSP 1 Score: 1464.9 bits (3791), Expect = 0.0e+00
Identity = 709/709 (100.00%), Postives = 709/709 (100.00%), Query Frame = 0

Query: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60
           MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK
Sbjct: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60

Query: 61  VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120
           VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP
Sbjct: 61  VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120

Query: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML 180
           LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML
Sbjct: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML 180

Query: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG 240
           HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG
Sbjct: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG 240

Query: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS 300
           KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS
Sbjct: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS 300

Query: 301 QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS 360
           QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS
Sbjct: 301 QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS 360

Query: 361 TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420
           TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT
Sbjct: 361 TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420

Query: 421 DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480
           DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS
Sbjct: 421 DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480

Query: 481 CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540
           CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF
Sbjct: 481 CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540

Query: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600
           LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF
Sbjct: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600

Query: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660
           SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND
Sbjct: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660

Query: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM 710
           ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM
Sbjct: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM 709

BLAST of CmaCh10G008100 vs. NCBI nr
Match: XP_023515681.1 (putative pumilio homolog 8, chloroplastic [Cucurbita pepo subsp. pepo])

HSP 1 Score: 1434.9 bits (3713), Expect = 0.0e+00
Identity = 694/709 (97.88%), Postives = 701/709 (98.87%), Query Frame = 0

Query: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60
           MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMV+DSCNGLSLDELHNNGSLENGK
Sbjct: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVADSCNGLSLDELHNNGSLENGK 60

Query: 61  VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120
           V IG+FGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNK SLKTEPSMVSDPP
Sbjct: 61  VSIGQFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKHSLKTEPSMVSDPP 120

Query: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML 180
           LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAG VPPLKTP+AMPFT HEQML
Sbjct: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGHVPPLKTPSAMPFTHHEQML 180

Query: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG 240
           HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG
Sbjct: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG 240

Query: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS 300
           KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNR+AHYAGSGVPNLVFTKPN+DTLDS
Sbjct: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRTAHYAGSGVPNLVFTKPNVDTLDS 300

Query: 301 QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS 360
           QEKSFNQ SPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYIS NGFGCPSCIVRQNGLPS
Sbjct: 301 QEKSFNQCSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYIS-NGFGCPSCIVRQNGLPS 360

Query: 361 TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420
           TTKDCICHDNLRASSTLSSY+NHPIEI PSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT
Sbjct: 361 TTKDCICHDNLRASSTLSSYKNHPIEIPPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420

Query: 421 DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480
           +GTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS
Sbjct: 421 EGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480

Query: 481 CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540
           CNMHGTRAVQKLIETLKT EQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF
Sbjct: 481 CNMHGTRAVQKLIETLKTPEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540

Query: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600
           LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF
Sbjct: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600

Query: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660
           SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND
Sbjct: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660

Query: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM 710
           ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM
Sbjct: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM 708

BLAST of CmaCh10G008100 vs. NCBI nr
Match: XP_022960755.1 (putative pumilio homolog 8, chloroplastic [Cucurbita moschata])

HSP 1 Score: 1431.0 bits (3703), Expect = 0.0e+00
Identity = 694/709 (97.88%), Postives = 699/709 (98.59%), Query Frame = 0

Query: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60
           MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELH NGSLENGK
Sbjct: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHKNGSLENGK 60

Query: 61  VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120
           V IG+FGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP
Sbjct: 61  VSIGQFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120

Query: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML 180
           LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAG VPPLKTP+AMPFT HEQML
Sbjct: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGHVPPLKTPSAMPFTHHEQML 180

Query: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG 240
           HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNL ESCG
Sbjct: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLLESCG 240

Query: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS 300
           KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNR+AHYAGSGVPNLVFTKPN+DTLDS
Sbjct: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRTAHYAGSGVPNLVFTKPNVDTLDS 300

Query: 301 QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS 360
           QEKSFNQ S RKVPMRTHGPIGVDCMKAPIGVDAEKLKYYIS NGFGCPSCIVRQNGLPS
Sbjct: 301 QEKSFNQCSLRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYIS-NGFGCPSCIVRQNGLPS 360

Query: 361 TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420
           TTKDCICHDNLRASSTLSSYENHPIEI PSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT
Sbjct: 361 TTKDCICHDNLRASSTLSSYENHPIEIPPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420

Query: 421 DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480
           +GTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS
Sbjct: 421 EGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480

Query: 481 CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540
           CNMHGTRAVQKLIETLKT EQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF
Sbjct: 481 CNMHGTRAVQKLIETLKTPEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540

Query: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600
           LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF
Sbjct: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQF 600

Query: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660
           SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND
Sbjct: 601 SLELGRCPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELIND 660

Query: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM 710
           ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM
Sbjct: 661 ERFSQIMLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTNM 708

BLAST of CmaCh10G008100 vs. NCBI nr
Match: KAG7023742.1 (Pumilio-like 12, partial [Cucurbita argyrosperma subsp. argyrosperma])

HSP 1 Score: 1195.3 bits (3091), Expect = 0.0e+00
Identity = 580/594 (97.64%), Postives = 585/594 (98.48%), Query Frame = 0

Query: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60
           MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK
Sbjct: 1   MGSKLKLELEKLFSEDLIVACDNEHAEESVHKGMSSMVSDSCNGLSLDELHNNGSLENGK 60

Query: 61  VLIGRFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120
           V IG+FGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP
Sbjct: 61  VSIGQFGDRSLTSAVEKLSLGGEGTGKTWMNHPNLMDDQFESYLNKQSLKTEPSMVSDPP 120

Query: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGQVPPLKTPAAMPFTRHEQML 180
           LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAG VPPLKTP+AMPFT HEQML
Sbjct: 121 LRPSHCVNNGYYEIRMPGLPPQISVEARPVSDVHKNGEAGHVPPLKTPSAMPFTHHEQML 180

Query: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLFESCG 240
           HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNL ESCG
Sbjct: 181 HSGLSPIHFMRPQQMNHGEIGSNCTKEQQLLSCRMQGQHQYLYDLHNQQREYSNLLESCG 240

Query: 241 KPFGSLRFRSPNQECFIEVPFSPYLGQFKHEGFCNRSAHYAGSGVPNLVFTKPNIDTLDS 300
           KPFGSLRFRSPNQE FIEVPFSPYLGQFKHEGFCNR+AHYAGSGVPNLVFTKPN+DTLDS
Sbjct: 241 KPFGSLRFRSPNQEGFIEVPFSPYLGQFKHEGFCNRTAHYAGSGVPNLVFTKPNVDTLDS 300

Query: 301 QEKSFNQSSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYISQNGFGCPSCIVRQNGLPS 360
           QEKSFNQ SPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYIS NGFGCPSCIVRQNGLPS
Sbjct: 301 QEKSFNQCSPRKVPMRTHGPIGVDCMKAPIGVDAEKLKYYIS-NGFGCPSCIVRQNGLPS 360

Query: 361 TTKDCICHDNLRASSTLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420
           TTKDCICHDNLRASSTLSSYENHPIEI PSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT
Sbjct: 361 TTKDCICHDNLRASSTLSSYENHPIEIPPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFT 420

Query: 421 DGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480
           +GTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS
Sbjct: 421 EGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQILYKINRPGELIRIS 480

Query: 481 CNMHGTRAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540
           CNMHGTRAVQKLIETLKT EQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF
Sbjct: 481 CNMHGTRAVQKLIETLKTPEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGF 540

Query: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYG 595
           LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYG
Sbjct: 541 LFDAAIKSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYG 593

BLAST of CmaCh10G008100 vs. TAIR 10
Match: AT5G56510.1 (pumilio 12 )

HSP 1 Score: 378.3 bits (970), Expect = 1.5e-104
Identity = 191/326 (58.59%), Postives = 244/326 (74.85%), Query Frame = 0

Query: 385 IEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMID 444
           + + P K NS+ EA GKIY +AKDQHGCRFLQR+F++    DIEMIF EII ++SELM+D
Sbjct: 265 LSLQPQKYNSIAEARGKIYYLAKDQHGCRFLQRIFSEKDGNDIEMIFNEIIDYISELMMD 324

Query: 445 PFGNYLIQKLLGVCDEDQRLQILYKINR-PGELIRISCNMHGTRAVQKLIETLKTQEQFS 504
           PFGNYL+QKLL VC+EDQR+QI++ I R PG LI+ISC+MHGTRAVQK++ET K +E+ S
Sbjct: 325 PFGNYLVQKLLEVCNEDQRMQIVHSITRKPGLLIKISCDMHGTRAVQKIVETAKREEEIS 384

Query: 505 MVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQ 564
           +++S LK GIV L+KNVNGNHV Q CLQYL+P    FLF+AAI  CVE+ATDRHGCCVLQ
Sbjct: 385 IIISALKHGIVHLIKNVNGNHVVQRCLQYLLPYCGKFLFEAAITHCVELATDRHGCCVLQ 444

Query: 565 KCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLE 624
           KCL  S+   +  L+SE+  NAL+LSQD +GNYV+Q+  EL      + W T  I ++LE
Sbjct: 445 KCLGYSEGEQKQHLVSEIASNALLLSQDPFGNYVLQYVFEL-----QLQWATFEILEQLE 504

Query: 625 GHFADLSIQKYSSNVVEKCAYTGED-YLFKIIDELINDERFSQIMLNPYGNYAVQAVLAR 684
           G++ +LS+QK SSNVVEKC    +D +  +II ELIN  R  Q+ML+PYGNY +QA L +
Sbjct: 505 GNYTELSMQKCSSNVVEKCLKLADDKHRARIIRELINYGRLDQVMLDPYGNYVIQAALKQ 564

Query: 685 SWNFKSPVHAKLVAAIRPHVPLLRTN 709
           S   K  VHA LV AI+ ++  LRTN
Sbjct: 565 S---KGNVHALLVDAIKLNISSLRTN 582

BLAST of CmaCh10G008100 vs. TAIR 10
Match: AT1G78160.1 (pumilio 7 )

HSP 1 Score: 322.4 bits (825), Expect = 9.8e-88
Identity = 165/342 (48.25%), Postives = 233/342 (68.13%), Query Frame = 0

Query: 369 DNLRASS-TLSSYENHPIEILPSKCNSLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDI 428
           D+ R+++  L S   +P EI      ++ +  G +YLMAKDQHGCRFLQR+F +GT  D 
Sbjct: 304 DHQRSNTRALMSNNGNPTEICHPSLPNMCDIQGYVYLMAKDQHGCRFLQRIFDEGTSVDA 363

Query: 429 EMIFGEIIHHVSELMIDPFGNYLIQKLLGVCDEDQRLQI-LYKINRPGELIRISCNMHGT 488
            +IF E+I HV ELM+DPFGNYL+QKLL VC E+QR QI L     PG+LIRIS N +GT
Sbjct: 364 MIIFNEVIAHVVELMMDPFGNYLMQKLLDVCTEEQRTQIVLVATEEPGQLIRISLNAYGT 423

Query: 489 RAVQKLIETLKTQEQFSMVVSLLKTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAI 548
           R VQ+L+ET+++ +Q S+V   L+ G + L+K++NGNHV Q CLQ L  +   F+FDAA 
Sbjct: 424 RVVQRLVETIRSGKQISLVKLALRPGFLDLIKDLNGNHVIQRCLQCLSTEDNKFIFDAAT 483

Query: 549 KSCVEVATDRHGCCVLQKCLSVSDARYRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGR 608
           K C E+AT RHGCCVLQKC++ S  + R++L++E+ RN+L+L+QD +GNY VQF +EL  
Sbjct: 484 KFCTEIATHRHGCCVLQKCIAYSMRQQREKLIAEISRNSLLLAQDPFGNYAVQFVIEL-- 543

Query: 609 CPTSIPWVTPGIFKRLEGHFADLSIQKYSSNVVEKCAYTGEDYLFKIIDELINDERFSQI 668
               IP     +  +L+GH+  LS+QK+SS++VE+C     +   +I+ EL++   F Q+
Sbjct: 544 ---RIPSAVAMMLAQLKGHYVQLSMQKFSSHMVERCLMHCPESRPQIVRELVSVPHFDQL 603

Query: 669 MLNPYGNYAVQAVLARSWNFKSPVHAKLVAAIRPHVPLLRTN 709
           + +PY N+ +QA LA +   K P+HA LV  IRPH  +LR N
Sbjct: 604 LQDPYANFVIQAALAAT---KGPLHASLVEVIRPH-SILRNN 636

BLAST of CmaCh10G008100 vs. TAIR 10
Match: AT1G22240.1 (pumilio 8 )

HSP 1 Score: 308.5 bits (789), Expect = 1.5e-83
Identity = 170/379 (44.85%), Postives = 244/379 (64.38%), Query Frame = 0

Query: 331 GVDAEKLKYYISQNGFGCPSCIVRQNGLPSTTKDCICHDNLRASSTLSSYENHPIEILPS 390
           GVD +  +   S N  G  SC +    +P   K       + A        ++P E LP 
Sbjct: 142 GVDQKDHQISNSWN-LGMQSCNMNNYSVPVHGK-----SGVGALFDHQGISSNPNESLP- 201

Query: 391 KCNSLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMIDPFGNYL 450
               + E  G +Y MAKDQHGCRFLQ +F DG+  D  +IF E+I HV ELM+DPFGNYL
Sbjct: 202 ---KVSEFQGYVYFMAKDQHGCRFLQWIFEDGSALDALVIFSEVIPHVVELMMDPFGNYL 261

Query: 451 IQKLLGVCDEDQRLQILYKI-NRPGELIRISCNMHGTRAVQKLIETLKTQEQFSMVVSLL 510
           +QKLL VC+E+QR QI+  + + PG+LIRIS N +GTR VQ+L+E++KT++Q S+V S L
Sbjct: 262 MQKLLDVCNEEQRTQIILMVTSEPGQLIRISLNAYGTRVVQRLVESIKTRKQISLVKSAL 321

Query: 511 KTGIVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQKCLSVS 570
           + G + L++++NGNHV Q CLQ L  +   F+F+ A K C+++AT RHGCCVLQKC++ S
Sbjct: 322 RPGFLNLIRDLNGNHVIQRCLQCLSTEDNEFIFEDATKFCIDIATHRHGCCVLQKCIAYS 381

Query: 571 DARYRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLEGHFADL 630
               R++L++E+ RN+L L+QD YGNY VQF LEL R  ++I      +  +L+GH+ +L
Sbjct: 382 SGLQREKLVTEISRNSLFLAQDPYGNYAVQFVLEL-RDFSAI----AAMLAQLKGHYVEL 441

Query: 631 SIQKYSSNVVEKCAYTGEDYLFKIIDELINDERFSQIMLNPYGNYAVQAVLARSWNFKSP 690
           S+QK+SS++VE+C     +   +I+ ELI+   F  ++ +PY N+ +QA LA +   K  
Sbjct: 442 SMQKFSSHMVERCLTHCPESRPQIVRELISVPHFDILIQDPYANFVIQAALAVT---KGS 501

Query: 691 VHAKLVAAIRPHVPLLRTN 709
           +HA LV  IRPH  +LR N
Sbjct: 502 LHATLVEVIRPH-SILRNN 501

BLAST of CmaCh10G008100 vs. TAIR 10
Match: AT4G08840.1 (pumilio 11 )

HSP 1 Score: 270.8 bits (691), Expect = 3.4e-72
Identity = 138/315 (43.81%), Postives = 208/315 (66.03%), Query Frame = 0

Query: 394 SLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQK 453
           S+ ++ G +YLMAKDQ GCR LQ+   +G   D+ +IF E+I++V EL  DPFGNYLIQK
Sbjct: 238 SMVDSYGSVYLMAKDQLGCRLLQKFVDEGNFVDVMIIFKEVINNVIELGTDPFGNYLIQK 297

Query: 454 LLGVCDEDQRLQILYKI-NRPGELIRISCNMHGTRAVQKLIETLKTQEQFSMVVSLLKTG 513
           L+ VC+E+QR QIL ++ ++PG L++IS N +GTR VQKLIET+ T+EQ S+V S L  G
Sbjct: 298 LIEVCNEEQRTQILIRLTSKPGLLVKISINNYGTRVVQKLIETVTTKEQISLVKSALVPG 357

Query: 514 IVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQKCLSVSDAR 573
            + L + +NGNHV  +CL++  P+   F+ +AA K C+E+AT RHGCCVLQ+C+S S   
Sbjct: 358 FLSLFRELNGNHVILNCLKFFSPNDNKFILEAATKFCIEIATTRHGCCVLQRCVSYSVGE 417

Query: 574 YRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLEGHFADLSIQ 633
             ++L+ E+ RN+L+L+QD +GNY+VQ+ +E       +  V   +   L G++  L+ Q
Sbjct: 418 QHEKLVDEISRNSLLLAQDPFGNYLVQYIIE-----KKVGGV--NVLFELRGNYVKLATQ 477

Query: 634 KYSSNVVEKCAYTGEDYLFKIIDELINDERFSQIMLNPYGNYAVQAVLARSWNFKSPVHA 693
           K+ S+VVEKC     +   +I++EL++   F  ++ +PY NY +Q  L+++  F   V A
Sbjct: 478 KFGSHVVEKCLRYYPESRSQIVNELVSVLNFGYLLQDPYANYVIQCALSKTKGF---VRA 537

Query: 694 KLVAAIRPHVPLLRT 708
            LV  +R +  L  T
Sbjct: 538 SLVEKVRRYENLKMT 542

BLAST of CmaCh10G008100 vs. TAIR 10
Match: AT1G35730.1 (pumilio 9 )

HSP 1 Score: 261.9 bits (668), Expect = 1.6e-69
Identity = 139/306 (45.42%), Postives = 198/306 (64.71%), Query Frame = 0

Query: 394 SLDEATGKIYLMAKDQHGCRFLQRMFTDGTKEDIEMIFGEIIHHVSELMIDPFGNYLIQK 453
           S+ E  G + LMAKDQ GCR LQ++  +GT  + ++I   II HV EL +DPFGNY++QK
Sbjct: 246 SMVEIYGSVNLMAKDQIGCRVLQKLVEEGTFHEAKVILLAIIDHVVELSMDPFGNYIVQK 305

Query: 454 LLGVCDEDQRLQILYKI-NRPGELIRISCNMHGTRAVQKLIETLKTQEQFSMVVSLLKTG 513
           L  V DE+QR  I+  + + P ELIRI  N +GTR VQK+IET+KT++Q ++V S LK G
Sbjct: 306 LFDVSDEEQRTLIVSVLTSNPRELIRICLNTYGTRVVQKMIETVKTKQQIALVKSGLKPG 365

Query: 514 IVILMKNVNGNHVAQHCLQYLMPDYIGFLFDAAIKSCVEVATDRHGCCVLQKCLSVSDAR 573
            + L+K++NGNHV Q CLQ L P+   F+ +AA K C E+A  RHGCCVLQ C+S S   
Sbjct: 366 FLALVKDLNGNHVIQSCLQTLGPNDNEFVLEAATKYCAEIAIHRHGCCVLQCCISNSVGL 425

Query: 574 YRDRLLSEVVRNALVLSQDQYGNYVVQFSLELGRCPTSIPWVTPGIFKRLEGHFADLSIQ 633
            R+RL++E+ RN+L LSQD +GNYVVQ+ ++              +  +   H+A+L+ Q
Sbjct: 426 QRERLVAEISRNSLHLSQDPFGNYVVQYLIDQQVSAVK-------LLVQFRMHYAELATQ 485

Query: 634 KYSSNVVEKCAYTGEDYLFKIIDELINDERFSQIMLNPYGNYAVQAVLARSWNFKSPVHA 693
           K+SS+V+EKC     +   +I+ EL+    F  ++ +PY NY +Q  L+ +   K PV A
Sbjct: 486 KFSSHVIEKCLRKYPESRAEIVRELLCVPNFEYLLQDPYANYVIQTALSVT---KGPVRA 541

Query: 694 KLVAAI 699
           KLVA +
Sbjct: 546 KLVAKV 541

The following BLAST results are available for this feature:
Match NameE-valueIdentityDescription
Q9LVC32.1e-10358.59Pumilio homolog 12 OS=Arabidopsis thaliana OX=3702 GN=APUM12 PE=2 SV=2[more]
Q9C9R61.4e-8648.25Putative pumilio homolog 7, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APU... [more]
Q9LM202.1e-8244.85Putative pumilio homolog 8, chloroplastic OS=Arabidopsis thaliana OX=3702 GN=APU... [more]
Q9LDW34.8e-7143.81Pumilio homolog 11 OS=Arabidopsis thaliana OX=3702 GN=APUM11 PE=3 SV=2[more]
Q1PFN92.2e-6845.42Pumilio homolog 9 OS=Arabidopsis thaliana OX=3702 GN=APUM9 PE=2 SV=1[more]
Match NameE-valueIdentityDescription
A0A6J1JGR00.0e+00100.00putative pumilio homolog 8, chloroplastic OS=Cucurbita maxima OX=3661 GN=LOC1114... [more]
A0A6J1HA050.0e+0097.88putative pumilio homolog 8, chloroplastic OS=Cucurbita moschata OX=3662 GN=LOC11... [more]
A0A0A0LZQ00.0e+0075.03PUM-HD domain-containing protein OS=Cucumis sativus OX=3659 GN=Csa_1G599460 PE=4... [more]
A0A5A7UN640.0e+0074.59Pumilio-like protein 12-like isoform X1 OS=Cucumis melo var. makuwa OX=1194695 G... [more]
A0A1S4DY700.0e+0074.19uncharacterized protein LOC103492417 OS=Cucumis melo OX=3656 GN=LOC103492417 PE=... [more]
Match NameE-valueIdentityDescription
KAG6590074.10.0e+0098.04Pumilio-like 12, partial [Cucurbita argyrosperma subsp. sororia][more]
XP_022987390.10.0e+00100.00putative pumilio homolog 8, chloroplastic [Cucurbita maxima][more]
XP_023515681.10.0e+0097.88putative pumilio homolog 8, chloroplastic [Cucurbita pepo subsp. pepo][more]
XP_022960755.10.0e+0097.88putative pumilio homolog 8, chloroplastic [Cucurbita moschata][more]
KAG7023742.10.0e+0097.64Pumilio-like 12, partial [Cucurbita argyrosperma subsp. argyrosperma][more]
Match NameE-valueIdentityDescription
AT5G56510.11.5e-10458.59pumilio 12 [more]
AT1G78160.19.8e-8848.25pumilio 7 [more]
AT1G22240.11.5e-8344.85pumilio 8 [more]
AT4G08840.13.4e-7243.81pumilio 11 [more]
AT1G35730.11.6e-6945.42pumilio 9 [more]
InterPro
Analysis Name: InterPro Annotations of Cucurbita maxima (Rimu) v1.1
Date Performed: 2021-10-25
IPR TermIPR DescriptionSourceSource TermSource DescriptionAlignment
NoneNo IPR availableCOILSCoilCoilcoord: 765..765
NoneNo IPR availablePANTHERPTHR12537RNA BINDING PROTEIN PUMILIO-RELATEDcoord: 5..709
NoneNo IPR availablePANTHERPTHR12537:SF147PUMILIO HOMOLOG 12coord: 5..709
IPR001313Pumilio RNA-binding repeatSMARTSM00025pum_5coord: 433..468
e-value: 7.3E-7
score: 38.8
coord: 508..543
e-value: 0.0012
score: 28.1
coord: 580..616
e-value: 10.0
score: 12.8
coord: 471..507
e-value: 1.2
score: 18.1
coord: 544..579
e-value: 2.7E-5
score: 33.6
coord: 621..655
e-value: 3.4
score: 16.4
coord: 658..697
e-value: 270.0
score: 1.8
coord: 397..432
e-value: 8.4E-5
score: 32.0
IPR001313Pumilio RNA-binding repeatPFAMPF00806PUFcoord: 663..681
e-value: 0.58
score: 9.9
coord: 437..469
e-value: 3.0E-8
score: 33.2
coord: 625..644
e-value: 0.058
score: 13.1
coord: 586..603
e-value: 0.0079
score: 15.9
coord: 402..435
e-value: 4.3E-11
score: 42.3
coord: 513..543
e-value: 0.079
score: 12.7
coord: 549..581
e-value: 2.2E-11
score: 43.2
coord: 478..507
e-value: 3.0E-5
score: 23.6
IPR001313Pumilio RNA-binding repeatPROSITEPS50302PUMcoord: 580..620
score: 7.777317
IPR001313Pumilio RNA-binding repeatPROSITEPS50302PUMcoord: 544..579
score: 9.387183
IPR001313Pumilio RNA-binding repeatPROSITEPS50302PUMcoord: 433..469
score: 11.347021
IPR001313Pumilio RNA-binding repeatPROSITEPS50302PUMcoord: 397..432
score: 8.523921
IPR001313Pumilio RNA-binding repeatPROSITEPS50302PUMcoord: 471..507
score: 8.477259
IPR011989Armadillo-like helicalGENE3D1.25.10.10coord: 382..718
e-value: 2.0E-105
score: 354.5
IPR033133Pumilio homology domainPROSITEPS50303PUM_HDcoord: 372..722
score: 78.828072
IPR033712Pumilio, RNA binding domainCDDcd07920Pumiliocoord: 396..706
e-value: 1.26482E-114
score: 347.657
IPR016024Armadillo-type foldSUPERFAMILY48371ARM repeatcoord: 393..709

Relationships

The following mRNA feature(s) are a part of this gene:

Feature NameUnique NameType
CmaCh10G008100.1CmaCh10G008100.1mRNA


GO Annotation
GO Assignments
This gene is annotated with the following GO terms.
Category Term Accession Term Name
biological_process GO:0006417 regulation of translation
cellular_component GO:0005737 cytoplasm
molecular_function GO:0003729 mRNA binding
molecular_function GO:0003723 RNA binding